RchiOBHm_Chr2g0085981

atfip1 v ,atfips5,fip1 v ,fips5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009289
Physical Location & Seq
Forward (+)
1216828 .. 1224812
7985 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46151

Sequence Viewer

Length: 3999 bp
ATGGAAGACGACGATGAATTCGGAGATCTCTACACCGACGTTCTCCAATCCTTCCAGTCATCATCCCAGCCGTCCTCCTCAGCTCCGGCGCCGCCGCAATCGTTCCACCGTCCGATCGATCTCAACCTCAAGACCGAAGACGCCGACGACGAGATCCTAGCCCCAGCTCCTCCTCCTCGCTCCCACGAGTCCAATCCCAATTCGCAAACCCTAGCTCCCGATTCGACCAGAGCCGCCGATTCGGGCGGCGGTGCTAGGGTTTTGGAGGCGAAAGACGTCAAATTGCAGAGTAAAGATTCGGATTTGACGGAAAAGGAGGTGAATTTCGATATCGAGGAGGAGGGGAACAATGGAATTTCCGGTCTGGGATTGGACGCCGTGATTCCGGGGCTTTCGGCCGCCGCGCCGGTGAACAACACGGCGGCGAATGTGGAGAACAACCCGGAAGGTTCGAGAAGGGACGGCGGCGGAGGCGGAGGCGGAGGCGATGATTGGGACAGTGATGATAGCGAGGATGATTTGCAGATTGTGTTGAATGATAACAACCCAATGGGGATGGAGAGAGGCAATGGCGAAGAGGATGACGACGACGGGTTGGTTATTGTGGCGGACAGTGAGCACAACGCGCCGGCGGAGGAGCAAGAGTGGGAAGAGGGGCAGCAGGGGGCGGCGGAGGGGGAGAGGAAGGAGATGGGGGAGGCAGGGAAAGGCGGAGGCGGCGGTCCGGTGGTGGCTCCGAAAATTGGGTATAGCAATCATGGGTATCATCCATTTCATTCCCAGTTTAAGTATGTGAGACCTGGTGCTGTGCCAATGCCTGGACCAACAACTTCTGGTCCTGGAGGAGTCCCGGGTCAAGTTCGTCCACTTGTAAACATGGGTCCAACACCTGGTCGAGGTAGAGGTGACTGGAGACCAACTGGATTAAAAAATGGTACTCCGCTGCAGAAAAATTTTCATTCGGGCTTTGGTACACCTGGTTGGGGAAACAATATGGGTGGTCGCGGTTTCGGGGGTGGACTTGAATTCACACTTCCTTCTCATAAGACCATATTTGATGTCGACATTGACGGTTTTGAGGAAAAACCTTGGAAATATCCTGGTGTTGATACTTCAGACTATTTCAACTTTGGATTGAATGAAGATAGCTGGAGAGATTATTGCAAACAGCTGGAACAGCTCCGCTTGGAGTCCACCATGCAAAGCAAAATCCGTGTTTATGAAAGTGGACGAACAGAACAGGAGTATGATCCAGATCTGCCTCCAGAATTGGCAGCAGCAACTGGTATGCATGACTTTCCTGCTGAAAATTCCAACCCTGGGAAGTCAGAGGGTGGGCAAAGTGATTTTGCAAAAGGATCTGCTCGCCTGCGTCCACCTATTCCAACTGGAAGAGCAATACAGGTGGAAAGTGGCTATGGTGAACGTTTCCCCTCAAGTGAAAACCGGCCACAGCGAATGCGTGACTCAGATGCTGTCATTGAAATTGTCTTGCAGGACTCTTTAGATGATGATTCTTCTGCAGGAAATGGTATCCCAGACGGAAGTGAAAACGATCCTTCAAAGGAGGGTGGAAGTGCTATTGGTGAGGGAGACCTTCCACAGGATGACAAGGCCTACTTCAATGGTTTTCCACAGGCTCACAATAATCGAAAGAGGGATCCACTTGAAAGAAAAAAGCCATTCAATGATAACGTGCCAGAAGATGATGAAAGCTTGCCCTTCCGTCCAGAAGGGCCAGTCCAACATGCTGGTTCTGGGGACCAGACTCCTAGTTCTGCTGGTGGTAGTTTTGGAGAAAATAGGGGGACACAGAGAAGAGCACATGACAGATCTCCTCGTTTGACTCCTAGCCGGAATACAAGAGACAAGAAATTTCCTGACGGTCAAAAGGAAGGATCAGTGGAAAGCGTGGATGGTAGACGTAGTCCGTTGATCTCATCTCCCGCCTCACATGGGGCTGCCCGCGAGTCAAGTGTCGAGCATAGAGGCGGTGACCAAGATGAGACTCTACTGGGTGATGAAAGCTCTGGAATGGAAAAGGAGGAAATGGCTGCGAATGTAAATGATGATGTCCATGATGGAGTTCCAAACTATCAAAAGTTGAGTTCACGAGTTGACCCGTCTGTTGATGAAGAACTTGATGATGGCGAAGACTCCAAGGCTGCAAGAAGTAGTGACAACAGCAAAGCAAGATCAGGAAGCAGTAGAGATTATCAGAAGTGGAGGGATGGGGTTGAGGAGGAAGTCATTCAAGGACGTTCAGCTCACTCAGGAGGCATTAAGAGTCACCTTGATGAAAAGGAACAGGGATTCCAAAGAAAAGGCCGTGATGGTAGACAGGAACTGGATAGAAGTCACATGTTAGTCAAAGGGAGGGAGGGTTCTTATCCGTACAGGGATTGGGATCCTAGCTCTGCTCATCATTTGCAATTGAAAAATGATGGGCTTAATAGGCGAAAAGAGAGGGAAAATCTTGACGGAGCATGGCAAAGGAGGGATGATGATCCTTATAGCAGAAGGATTAGAACTGAAGAACCAAGGAAGAGGGAACGTGGTGATGAAATGGGATCCAGGCACAGGAGTAAGGTTCGTGAAAGCGAGAGGAGTGACAAAGATGAATATATGCAGTCAAGAAAGCAGTTAGATAATGGCAGCTATAGGGTCTTTTATGATAAGGATGTTGGATCACGACCCAGGGAAAGAGAAGATAGTTTGAAGGGCAGGTATGAACATATAGATGATTACCATGGCAAGAGAAGGAAAGATGAGGAATACATGAGGAGGGACCAAATAGATAAAGAAGAACTTCTGCAGGGCCACAGAGATACTACCACTCGCCGAAAGCGGGAAAGGGATGAAGTTTTAGATCAGCGTAAAAGAGATGACCAGCAGAAAGTCAGGGATAATCCTGATGACCACCACTCTGTCAGGCACAAAGATGAAAGCTGGTTGCCGAGGGAGAGAGGTGACAGACAAAGAGAAAGGGAGGAGTGGCATAGGCTTAAGCAATCCCATGAAGAAAATATACCCAAGAGGGAAAGAGATGATGGACGAGTTTCTGTAAGGGGTGGGCGTGCTACAGAAGACAAAGCATGGGTAGGCCATGCTAGGGCAAAGGATGAGAACAAAGGCTCTGATAAGGAGTACCAATATAAAGAGACTGTGCGACACAGTGAGCAGTCTAAAAGAAGGGATAGGGTTGAGGAGGAAAGTTCACATCATAGGGGGCGTGAGGATGCTCATGCACGGGGAAATCAAATGAATATTGATGAGAGGAGATCTGGCAAGGAGAGATCAAGTACTCGCAGTGAACGTGGTGATAGCCAAAAAGTCCAAGATAGAAAACATAAAGAGAATTCAAGAAGGAATAAAGAAACAGAAATTGCTGATATCAACACTTCAGTCACTTCCAAGAGACATCAAGAAGACCAAAGTGGTCGTAGCAAGGAGATGGGCTTGAAAGGCACCCGTGAGCAAGGACCTGGTCACTCTTCTAAAAGGCACAGGGAAGATGCTTCCTCTGATGATGAGCAGCAGGATTTAAAAAAGGGGCGCTCCAAATTGGAACGATGGACAAGTCATAAGGAGAGGGATTTCAGTATCAACAGTAAGGTATCCTCTACCTTAAAGTTCAAAGAGCTTGATAGGGGATCCTCAGATGCCAGTAAACTTCCAGATGATTCCAAGCCAGTTGAGGCTGTTGATAACCATCACCCGTTGGCTGAGGAGAATGCTGGTGATCAGGATTTTAAGGATGCTGACACAAAACCATTAGATACAGACACGGCATTGGAGGGTCGTCACCTGGACACAGTTGAGAAGTTGAAGAAACGAAGTGAGCGCTTCAAGCATCCATTGCCAAGTGAAAAAGAACCCTCAACAATTAAAAAGACTGAGACTGAAGTGCTGCCAACCCCTAATAGTGACCCTCCTGTGGTGGAATCTGAGATTAAACCAGAACGGCCAGCTCGGAAAAGAAGATGGATCAGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0042802 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fip1

Protein Analysis

1332

Amino Acids

149.35

Weight (kDa)

5.39

Isoelectric Point (pI)

58.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fip1 PF05182 353 - 395 2.6e-21 Fip1 motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 2715
AccB1I GGYRCC 2 cut(s) 88, 3467
AccB7I CCANNNNNTGG 2 cut(s) 818, 890
AccI GTMKAC 3 cut(s) 1062, 1921, 2338
AccII CGCG 4 cut(s) 404, 626, 1005, 1968
AclI AACGTT 1 cut(s) 1426
AcoI YGGCCR 3 cut(s) 396, 1448, 3965
AcsI RAATTY 8 cut(s) 17, 322, 354, 952, 1025, 1309, 1874, 3358
AcuI CTGAAG 4 cut(s) 1098, 2553, 3387, 3924
AcyI GRCGYC 4 cut(s) 89, 141, 276, 375
AfaI GTAC 5 cut(s) 937, 973, 2396, 3149, 3304
AfeI AGCGCT 1 cut(s) 3845
AflII CTTAAG 1 cut(s) 3005
AflIII ACRYGT 1 cut(s) 2361
AloI GAACNNNNNNTCC 4 cut(s) 2298, 2330, 2368, 2400
Alw21I GWGCWC 2 cut(s) 621, 1825
Alw26I GTCTC 8 cut(s) 790, 907, 1587, 1860, 2000, 3155, 3412, 3893
AlwNI CAGNNNCTG 3 cut(s) 1283, 1475, 2347
Ama87I CYCGRG 1 cut(s) 850
Aor51HI AGCGCT 1 cut(s) 3845
AoxI GGCC 8 cut(s) 396, 1448, 1614, 1736, 2326, 2818, 3103, 3965
ApoI RAATTY 8 cut(s) 17, 322, 354, 952, 1025, 1309, 1874, 3358
ArsI GACNNNNNNTTYG 1 cut(s) 34
Asp700I GAANNNNTTC 2 cut(s) 2250, 2808
AspLEI GCGC 5 cut(s) 91, 406, 628, 3558, 3846
AspS9I GGNCC 9 cut(s) 722, 821, 836, 881, 1736, 1762, 2788, 2818, 3482
AsuC2I CCSGG 4 cut(s) 387, 443, 851, 852
AvaI CYCGRG 1 cut(s) 850
AvaII GGWCC 7 cut(s) 722, 821, 836, 881, 1762, 2788, 3482
BamHI GGATCC 4 cut(s) 1660, 2407, 2570, 3653
BanI GGYRCC 2 cut(s) 88, 3467
BarI GAAGNNNNNNTAC 2 cut(s) 3012, 3044
BauI CACGAG 2 cut(s) 185, 2112
BbsI GAAGAC 5 cut(s) 12, 144, 2160, 3093, 3435
Bbv12I GWGCWC 2 cut(s) 621, 1825
BbvCI CCTCAGC 2 cut(s) 79, 3726
BceAI ACGGC 7 cut(s) 55, 362, 435, 478, 2313, 3804, 3980
BciVI GTATCC 2 cut(s) 1544, 3628
BclI TGATCA 1 cut(s) 3742
BcnI CCSGG 4 cut(s) 387, 443, 851, 852
BcoDI GTCTC 8 cut(s) 790, 907, 1587, 1860, 2000, 3155, 3412, 3893
BfaI CTAG 7 cut(s) 158, 212, 255, 1773, 1851, 2412, 3111
BfmI CTRYAG 5 cut(s) 944, 1521, 2659, 2813, 3081
BfoI RGCGCY 3 cut(s) 92, 3559, 3847
BfrI CTTAAG 1 cut(s) 3005
BfuAI ACCTGC 1 cut(s) 2715
BfuI GTATCC 2 cut(s) 1544, 3628
BglII AGATCT 4 cut(s) 25, 1255, 1832, 3281
BmcAI AGTACT 1 cut(s) 3304
Bme18I GGWCC 7 cut(s) 722, 821, 836, 881, 1762, 2788, 3482
BmeT110I CYCGRG 1 cut(s) 850
BmgT120I GGNCC 9 cut(s) 722, 821, 836, 881, 1736, 1762, 2788, 2818, 3482
BmrI ACTGGG 2 cut(s) 775, 2024
BmsI GCATC 6 cut(s) 1462, 3229, 3505, 3652, 3748, 3862
BmuI ACTGGG 2 cut(s) 775, 2024
BpiI GAAGAC 5 cut(s) 12, 144, 2160, 3093, 3435
BpmI CTGGAG 4 cut(s) 861, 931, 1171, 1248
Bpu10I CCTNAGC 2 cut(s) 79, 3726
BpuEI CTTGAG 2 cut(s) 113, 1420
BpuMI CCSGG 4 cut(s) 387, 443, 851, 852
Bsa29I ATCGAT 1 cut(s) 117
BsaBI GATNNNNATC 4 cut(s) 1254, 2406, 2505, 3711
BsaHI GRCGYC 4 cut(s) 89, 141, 276, 375
BsaI GGTCTC 3 cut(s) 790, 907, 1587
BsaWI WCCGGW 2 cut(s) 359, 724
BsaXI ACNNNNNCTCC 6 cut(s) 199, 229, 629, 659, 2368, 2398
Bse118I RCCGGY 3 cut(s) 406, 628, 1446
Bse3DI GCAATG 2 cut(s) 574, 3857
Bse8I GATNNNNATC 4 cut(s) 1254, 2406, 2505, 3711
BseCI ATCGAT 1 cut(s) 117
BseJI GATNNNNATC 4 cut(s) 1254, 2406, 2505, 3711
BseMI GCAATG 2 cut(s) 574, 3857
BseMII CTCAG 7 cut(s) 93, 1482, 2286, 3672, 3717, 3888, 3939
BseX3I CGGCCG 1 cut(s) 396
BseYI CCCAGC 2 cut(s) 66, 163
Bsh1236I CGCG 4 cut(s) 404, 626, 1005, 1968
Bsh1285I CGRYCG 2 cut(s) 117, 399
BshFI GGCC 8 cut(s) 398, 1450, 1616, 1738, 2328, 2820, 3105, 3967
BshNI GGYRCC 2 cut(s) 88, 3467
BshVI ATCGAT 1 cut(s) 117
BsiEI CGRYCG 2 cut(s) 117, 399
BsiHKAI GWGCWC 2 cut(s) 621, 1825
BsiHKCI CYCGRG 1 cut(s) 850
BslFI GGGAC 6 cut(s) 473, 509, 833, 1775, 1822, 2801
BsmAI GTCTC 8 cut(s) 790, 907, 1587, 1860, 2000, 3155, 3412, 3893
BsmFI GGGAC 6 cut(s) 473, 509, 833, 1775, 1822, 2801
BsmI GAATGC 2 cut(s) 1464, 3739
BsnI GGCC 8 cut(s) 398, 1450, 1616, 1738, 2328, 2820, 3105, 3967
Bso31I GGTCTC 3 cut(s) 790, 907, 1587
BsoBI CYCGRG 1 cut(s) 850
Bsp1286I GDGCHC 2 cut(s) 621, 1825
Bsp19I CCATGG 1 cut(s) 2749
BspANI GGCC 8 cut(s) 398, 1450, 1616, 1738, 2328, 2820, 3105, 3967
BspCNI CTCAG 7 cut(s) 92, 1481, 2285, 3671, 3718, 3889, 3940
BspDI ATCGAT 1 cut(s) 117
BspFNI CGCG 4 cut(s) 404, 626, 1005, 1968
BspMAI CTGCAG 3 cut(s) 948, 1525, 2817
BspMI ACCTGC 1 cut(s) 2715
BspQI GCTCTTC 2 cut(s) 1387, 1813
BspT107I GGYRCC 2 cut(s) 88, 3467
BspTI CTTAAG 1 cut(s) 3005
BspTNI GGTCTC 3 cut(s) 790, 907, 1587
BsrDI GCAATG 2 cut(s) 574, 3857
BsrFI RCCGGY 3 cut(s) 406, 628, 1446
BssAI RCCGGY 3 cut(s) 406, 628, 1446
BssNI GRCGYC 4 cut(s) 89, 141, 276, 375
BssSI CACGAG 2 cut(s) 185, 2112
BssT1I CCWWGG 4 cut(s) 1088, 2160, 2540, 2749
Bst2BI CACGAG 2 cut(s) 185, 2112
Bst4CI ACNGT 9 cut(s) 110, 500, 614, 1073, 1886, 3166, 3176, 3611, 3817
Bst6I CTCTTC 6 cut(s) 570, 645, 1387, 1813, 2540, 3499
BstACI GRCGYC 4 cut(s) 89, 141, 276, 375
BstAFI CTTAAG 1 cut(s) 3005
BstAPI GCANNNNNTGC 1 cut(s) 3859
BstC8I GCNNGC 7 cut(s) 630, 1366, 1370, 1718, 1966, 3078, 3969
BstDEI CTNAG 7 cut(s) 79, 1468, 2272, 3658, 3726, 3897, 3948
BstDSI CCRYGG 1 cut(s) 2749
BstEII GGTNACC 1 cut(s) 1994
BstENI CCTNNNNNAGG 2 cut(s) 894, 1601
BstFNI CGCG 4 cut(s) 404, 626, 1005, 1968
BstH2I RGCGCY 3 cut(s) 92, 3559, 3847
BstHHI GCGC 5 cut(s) 91, 406, 628, 3558, 3846
BstMAI GTCTC 8 cut(s) 790, 907, 1587, 1860, 2000, 3155, 3412, 3893
BstMCI CGRYCG 2 cut(s) 117, 399
BstMWI GCNNNNNNNGC 8 cut(s) 471, 625, 717, 1177, 2455, 3465, 3850, 3859
BstNSI RCATGY 2 cut(s) 1751, 2365
BstPI GGTNACC 1 cut(s) 1994
BstSFI CTRYAG 5 cut(s) 944, 1521, 2659, 2813, 3081
BstUI CGCG 4 cut(s) 404, 626, 1005, 1968
BstV2I GAAGAC 5 cut(s) 12, 144, 2160, 3093, 3435
BstXI CCANNNNNNTGG 1 cut(s) 1751
BstZI CGGCCG 1 cut(s) 396
Bsu15I ATCGAT 1 cut(s) 117
BsuI GTATCC 2 cut(s) 1544, 3628
BsuRI GGCC 8 cut(s) 398, 1450, 1616, 1738, 2328, 2820, 3105, 3967
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 2749
BtgZI GCGATG 1 cut(s) 501
BtsI GCAGTG 1 cut(s) 3316
BtsIMutI CAGTG 5 cut(s) 505, 619, 1908, 3181, 3316
BveI ACCTGC 1 cut(s) 2715
Cac8I GCNNGC 7 cut(s) 630, 1366, 1370, 1718, 1966, 3078, 3969
CaiI CAGNNNCTG 3 cut(s) 1283, 1475, 2347
CfoI GCGC 5 cut(s) 91, 406, 628, 3558, 3846
Cfr10I RCCGGY 3 cut(s) 406, 628, 1446
Cfr13I GGNCC 9 cut(s) 722, 821, 836, 881, 1736, 1762, 2788, 2818, 3482
Cfr9I CCCGGG 1 cut(s) 850
ClaI ATCGAT 1 cut(s) 117
CpoI CGGWCCG 1 cut(s) 722
CseI GACGC 3 cut(s) 149, 383, 1361
CsiI ACCWGGT 4 cut(s) 799, 889, 976, 3484
Csp6I GTAC 5 cut(s) 936, 972, 2395, 3148, 3303
CspCI CAANNNNNGTGG 4 cut(s) 979, 1014, 1386, 1421
CspI CGGWCCG 1 cut(s) 722
CviQI GTAC 5 cut(s) 936, 972, 2395, 3148, 3303
DdeI CTNAG 7 cut(s) 79, 1468, 2272, 3658, 3726, 3897, 3948
DinI GGCGCC 1 cut(s) 90
DraI TTTAAA 1 cut(s) 3546
EaeI YGGCCR 3 cut(s) 396, 1448, 3965
EagI CGGCCG 1 cut(s) 396
Eam1104I CTCTTC 6 cut(s) 570, 645, 1387, 1813, 2540, 3499
EarI CTCTTC 6 cut(s) 570, 645, 1387, 1813, 2540, 3499
EciI GGCGGA 7 cut(s) 483, 489, 495, 623, 647, 686, 726
EclXI CGGCCG 1 cut(s) 396
Eco130I CCWWGG 4 cut(s) 1088, 2160, 2540, 2749
Eco147I AGGCCT 1 cut(s) 1616
Eco31I GGTCTC 3 cut(s) 790, 907, 1587
Eco32I GATATC 2 cut(s) 331, 3394
Eco47I GGWCC 7 cut(s) 722, 821, 836, 881, 1762, 2788, 3482
Eco47III AGCGCT 1 cut(s) 3845
Eco52I CGGCCG 1 cut(s) 396
Eco57I CTGAAG 4 cut(s) 1098, 2553, 3387, 3924
Eco88I CYCGRG 1 cut(s) 850
Eco91I GGTNACC 1 cut(s) 1994
EcoNI CCTNNNNNAGG 2 cut(s) 894, 1601
EcoO109I RGGNCCY 1 cut(s) 3482
EcoO65I GGTNACC 1 cut(s) 1994
EcoRI GAATTC 3 cut(s) 17, 1025, 3358
EcoRV GATATC 2 cut(s) 331, 3394
EcoT14I CCWWGG 4 cut(s) 1088, 2160, 2540, 2749
EcoT22I ATGCAT 1 cut(s) 1293
EgeI GGCGCC 1 cut(s) 90
EheI GGCGCC 1 cut(s) 90
ErhI CCWWGG 4 cut(s) 1088, 2160, 2540, 2749
FalI AAGNNNNNCTT 4 cut(s) 1604, 1636, 2793, 2825
FaqI GGGAC 6 cut(s) 473, 509, 833, 1775, 1822, 2801
FauI CCCGC 3 cut(s) 1954, 1973, 2841
FbaI TGATCA 1 cut(s) 3742
FblI GTMKAC 3 cut(s) 1062, 1921, 2338
FspBI CTAG 7 cut(s) 158, 212, 255, 1773, 1851, 2412, 3111
GlaI GCGC 5 cut(s) 90, 405, 627, 3557, 3845
GsaI CCCAGC 2 cut(s) 70, 167
GsuI CTGGAG 4 cut(s) 861, 931, 1171, 1248
HaeII RGCGCY 3 cut(s) 92, 3559, 3847
HaeIII GGCC 8 cut(s) 398, 1450, 1616, 1738, 2328, 2820, 3105, 3967
HgaI GACGC 3 cut(s) 149, 383, 1361
HhaI GCGC 5 cut(s) 91, 406, 628, 3558, 3846
Hin1I GRCGYC 4 cut(s) 89, 141, 276, 375
Hin6I GCGC 5 cut(s) 89, 404, 626, 3556, 3844
HinP1I GCGC 5 cut(s) 89, 404, 626, 3556, 3844
HincII GTYRAC 2 cut(s) 1063, 2119
HindII GTYRAC 2 cut(s) 1063, 2119
HindIII AAGCTT 1 cut(s) 1714
Hpy99I CGWCG 6 cut(s) 14, 41, 149, 152, 590, 593
HpyCH4III ACNGT 9 cut(s) 110, 500, 614, 1073, 1886, 3166, 3176, 3611, 3817
HpyCH4IV ACGT 8 cut(s) 39, 276, 1426, 1695, 1924, 2260, 2554, 3316
HpyF10VI GCNNNNNNNGC 8 cut(s) 471, 625, 717, 1177, 2455, 3465, 3850, 3859
HpyF3I CTNAG 7 cut(s) 79, 1468, 2272, 3658, 3726, 3897, 3948
HpySE526I ACGT 8 cut(s) 39, 276, 1426, 1695, 1924, 2260, 2554, 3316
Hsp92I GRCGYC 4 cut(s) 89, 141, 276, 375
HspAI GCGC 5 cut(s) 89, 404, 626, 3556, 3844
KasI GGCGCC 1 cut(s) 88
KroI GCCGGC 1 cut(s) 628
KroNI GCCGGC 1 cut(s) 630
Ksp22I TGATCA 1 cut(s) 3742
LguI GCTCTTC 2 cut(s) 1387, 1813
LmnI GCTCC 9 cut(s) 88, 172, 185, 220, 637, 739, 1185, 2483, 3563
LweI GCATC 6 cut(s) 1462, 3229, 3505, 3652, 3748, 3862
MabI ACCWGGT 4 cut(s) 799, 889, 976, 3484
MaeI CTAG 7 cut(s) 158, 212, 255, 1773, 1851, 2412, 3111
MaeII ACGT 8 cut(s) 39, 276, 1426, 1695, 1924, 2260, 2554, 3316
MfeI CAATTG 1 cut(s) 2432
MhlI GDGCHC 2 cut(s) 621, 1825
Mly113I GGCGCC 1 cut(s) 89
MmeI TCCRAC 5 cut(s) 908, 1338, 1409, 1768, 2665
Mph1103I ATGCAT 1 cut(s) 1293
MreI CGCCGGCG 1 cut(s) 628
MroNI GCCGGC 1 cut(s) 628
MroXI GAANNNNTTC 2 cut(s) 2250, 2808
MslI CAYNNNNRTG 3 cut(s) 2295, 2739, 2940
MspA1I CMGCKG 2 cut(s) 943, 1171
MspCI CTTAAG 1 cut(s) 3005
MunI CAATTG 1 cut(s) 2432
Mva1269I GAATGC 2 cut(s) 1464, 3739
MvnI CGCG 4 cut(s) 404, 626, 1005, 1968
MwoI GCNNNNNNNGC 8 cut(s) 471, 625, 717, 1177, 2455, 3465, 3850, 3859
NaeI GCCGGC 1 cut(s) 630
NarI GGCGCC 1 cut(s) 89
NciI CCSGG 4 cut(s) 387, 443, 851, 852
NcoI CCATGG 1 cut(s) 2749
NgoMIV GCCGGC 1 cut(s) 628
NmeAIII GCCGAG 1 cut(s) 2982
NsiI ATGCAT 1 cut(s) 1293
NspI RCATGY 2 cut(s) 1751, 2365
PasI CCCWGGG 2 cut(s) 1319, 2697
PceI AGGCCT 1 cut(s) 1616
PciI ACATGT 1 cut(s) 2361
PciSI GCTCTTC 2 cut(s) 1387, 1813
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 3313
PctI GAATGC 2 cut(s) 1464, 3739
PdiI GCCGGC 1 cut(s) 630
PdmI GAANNNNTTC 2 cut(s) 2250, 2808
PfeI GAWTC 8 cut(s) 221, 239, 296, 382, 1514, 2313, 3683, 3944
PflFI GACNNNGTC 2 cut(s) 1926, 3486
PflMI CCANNNNNTGG 2 cut(s) 818, 890
PfoI TCCNGGA 1 cut(s) 838
Ple19I CGATCG 1 cut(s) 117
PluTI GGCGCC 1 cut(s) 92
PpuMI RGGWCCY 1 cut(s) 3482
PscI ACATGT 1 cut(s) 2361
Psp1406I AACGTT 1 cut(s) 1426
Psp5II RGGWCCY 1 cut(s) 3482
PspEI GGTNACC 1 cut(s) 1994
PspFI CCCAGC 2 cut(s) 66, 163
PspPI GGNCC 9 cut(s) 722, 821, 836, 881, 1736, 1762, 2788, 2818, 3482
PspPPI RGGWCCY 1 cut(s) 3482
PstI CTGCAG 3 cut(s) 948, 1525, 2817
PstNI CAGNNNCTG 3 cut(s) 1283, 1475, 2347
PsyI GACNNNGTC 2 cut(s) 1926, 3486
PvuI CGATCG 1 cut(s) 117
PvuII CAGCTG 1 cut(s) 1171
RsaI GTAC 5 cut(s) 937, 973, 2396, 3149, 3304
RsaNI GTAC 5 cut(s) 936, 972, 2395, 3148, 3303
RseI CAYNNNNRTG 3 cut(s) 2295, 2739, 2940
Rsr2I CGGWCCG 1 cut(s) 722
RsrII CGGWCCG 1 cut(s) 722
SalI GTCGAC 1 cut(s) 1061
SapI GCTCTTC 2 cut(s) 1387, 1813
Sau96I GGNCC 9 cut(s) 722, 821, 836, 881, 1736, 1762, 2788, 2818, 3482
ScaI AGTACT 1 cut(s) 3304
SduI GDGCHC 2 cut(s) 621, 1825
SexAI ACCWGGT 4 cut(s) 799, 889, 976, 3484
SfaNI GCATC 6 cut(s) 1462, 3229, 3505, 3652, 3748, 3862
SfcI CTRYAG 5 cut(s) 944, 1521, 2659, 2813, 3081
SfoI GGCGCC 1 cut(s) 90
SgrAI CRCCGGYG 2 cut(s) 406, 628
SinI GGWCC 7 cut(s) 722, 821, 836, 881, 1762, 2788, 3482
SmaI CCCGGG 1 cut(s) 852
SmiMI CAYNNNNRTG 3 cut(s) 2295, 2739, 2940
SmlI CTYRAG 3 cut(s) 128, 1435, 3005
SmoI CTYRAG 3 cut(s) 128, 1435, 3005
SseBI AGGCCT 1 cut(s) 1616
SspDI GGCGCC 1 cut(s) 88
SspI AATATT 1 cut(s) 3268
SspMI CTAG 7 cut(s) 158, 212, 255, 1773, 1851, 2412, 3111
StuI AGGCCT 1 cut(s) 1616
StyI CCWWGG 4 cut(s) 1088, 2160, 2540, 2749
TaaI ACNGT 9 cut(s) 110, 500, 614, 1073, 1886, 3166, 3176, 3611, 3817
TaiI ACGT 8 cut(s) 42, 279, 1429, 1698, 1927, 2263, 2557, 3319
TaqI TCGA 9 cut(s) 117, 224, 327, 333, 452, 895, 1062, 1651, 1980
TaqII GACCGA 1 cut(s) 149
TatI WGTACW 1 cut(s) 3302
TfiI GAWTC 8 cut(s) 221, 239, 296, 382, 1514, 2313, 3683, 3944
TscAI CASTG 5 cut(s) 505, 619, 1908, 3181, 3316
TspGWI ACGGA 7 cut(s) 323, 1202, 1557, 1715, 1920, 2382, 2496
TspMI CCCGGG 1 cut(s) 850
TspRI CASTG 5 cut(s) 505, 619, 1908, 3181, 3316
Tth111I GACNNNGTC 2 cut(s) 1926, 3486
Van91I CCANNNNNTGG 2 cut(s) 818, 890
Vha464I CTTAAG 1 cut(s) 3005
VpaK11BI GGWCC 7 cut(s) 722, 821, 836, 881, 1762, 2788, 3482
XagI CCTNNNNNAGG 2 cut(s) 894, 1601
XapI RAATTY 8 cut(s) 17, 322, 354, 952, 1025, 1309, 1874, 3358
XceI RCATGY 2 cut(s) 1751, 2365
XmaI CCCGGG 1 cut(s) 850
XmiI GTMKAC 3 cut(s) 1062, 1921, 2338
XmnI GAANNNNTTC 2 cut(s) 2250, 2808
XspI CTAG 7 cut(s) 158, 212, 255, 1773, 1851, 2412, 3111
ZraI GACGTC 1 cut(s) 277
ZrmI AGTACT 1 cut(s) 3304
Zsp2I ATGCAT 1 cut(s) 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.