Rh2BG014500

atfip1 v ,atfips5,fip1 v ,fips5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009289
Physical Location & Seq
Forward (+)
1080650 .. 1088660
8011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG014500.1

Sequence Viewer

Length: 3465 bp
ATGGAAGACGACGATGAATTCGGAGATCTCTACACCGACGTTCTCCAATCCTTCCAGTCATCATCCCAGCCGTCCTCCTCAGCTCCGGCGCCGCCGCAATCGCTCCACCGTCCGATCGATCTCAGCCTCAAGACCGAAGACGAGATCCTAGCCCCAGCTCCTCCTCCTCGCTCCCACGAGTCCAATCCCAATTCGCAAACCCTAGCTCCCGATTCGACCAGAGCCGCCGATTCGGGCGGAGGCTCTAGGGTTTTGGAGGCGAAAGACGTCAAATTGCAGAGTAAAGATTCGGATTTGACGGAAAAGGAGGTGAATTTCGATATCGAGGAGGAGGGGAACAATGGAATTTCCGGTCTGGGACTGGACGCCGTGATTCCGGGGCTTTCGGCCGCCGCGCCGGTGAATAATACGGTGGAGAATAGCCGGGAAGATTCGAGAAGAGACGGCGGCGGAGGCGATGATTGGGACAGTGATGATAGTGAGGATGATTTGCAGATTGTGTTGAATGATAACAACCCAATGGGGATGGAGAGAGGAGGCAATGGCGAAGAGGATGACGACGACGGGCTGGTTATTGTGGCGGACAGTGAGCACAACGGGCCGGCGGAGGAGCAAGAGTGGGAAGAGGGGCAGCAGGGGGCGGCGGAGGGGGAGAGGAAGGAGATTGGGGAGGCAGGGAAAGGCGGAGGAGGCGGTCCGGTGGTGGCGCCGAAAATTGGGTATAGCAATCATGGGTATCATCCATTTCATTCCCAGTTTAAGTATGTGAGACCTGGTGCTGTGCCAATGCCTGGACCAGCAACTTCTGGTCCTGGAGGAGTCCCGGGTCAAGTTCGTCCACTTGTAAACATGGGTCCAACACCTGGTCGAGGTAGAGGTGACTGGAGACCAACTGGATTAAAAAATGGTACTCCGCTGCAGAAAAATTTTCATTCGGGCTTTGGTACACCTGGTTGGGGAAACAATATGGGTGGTCGCGGTTTCGGGGGTGGACTTGAATTCACACTTCCTTCTCACAAGACCATATTTGATGTCGACATTGACGGTTTTGAGGAAAAACCTTGGAAATATCCTGGTGTTGATACTTCAGACTATTTCAACTTTGGATTGAATGAAGATAGCTGGAGAGATTATTGCAAACAGCTGGAACAGCTCCGCTTGGAGTCCACCATGCAAAGCAAAATCCGTGTTTATGAAAGTGGACGAACAGAACAGGAGTATGATCCAGATCTGCCTCCAGAATTGGCAGCAGCAACTGGTATGCATGACTTTCCTGCTGAAAATTCCAACCCTGGGAAGTCAGAGGGTGGGCAAAGTGATTTTGCAAAAGGATCTGCTCGCCTGCGTCCACCTATTCCAACTGGAAGAGCAATACAGGTGGAAAGTGGTTATGGTGAACGTTTCCCCTCAAGTGAAAACCGGCCACAGCGAATGCGTGACTCAGATGCTGTCATTGAAATTGTCTTGCAGGACTCTTTAGATGATGATTCTTCTGCAGGAAATGGTATCCCAGACGGAAGTGAAAACGATCCTTCAAAGGAGGGTGGAAGTGCTATTGGTGAGGGAGACGTTCCACAGGATGACAAGGCCTACTTCAATGGTTTTCCACAGGCTCACAATAATCGAAAGAGGGATCCACTTGAAAGAAAAAAGCCATTCAATGATAACGTGCCAGAAGATGATGAAAGCTTGCCCTTCCGTCCAGAAGGGCCAGTCCAACATGCTGGTTCTGGGGACCAGACTCCCAGTTCTGCTGGTGGTAGTTTTGGAGAAAATAGGGGGATACAGAGAAGAGCACGTGACAGATCTCCTCGTTTGACTCCTAGCCGGAATACAAGAGACAAGAAATTTCCTGACAGTCAAAAGGAAGGATCAGTGGAAAGCGTGGATGGTAGACATAGTCCGTTGATCTCATCTCCCGTCTCACAGGGGGCTGCCCGCGAGTCAAGTGTCGAGCATAGAGGCGGTGACCAAGATGAGACTCTGCTAGGTGATGAAAGCTCTGGAATGGAAAAGGAGGAAATGGCTGCGAATGTAACTGATGATGTCCATGATGGAGTTCCAAACTATCAAAAGTTGAGTTCACGAGTTGACCAGTCTGCTGATGAAGAACTTGATGATGGCGAAGACTCCAAGGCTGCAAGAAGTAGTGACAACAGCAAAGCAAGATCAGGAAGCAGTAGAGATTATCAGAAGTGGAGGGATGGGGTTGAGGAGGAAGTCATTCAAGGACGTTCAGCTCACTCGGGAGGCATCAAGAGTCACCTTGATGAAAAGGAACAGGGATTCCAAAGAAAAGGCCGTGATGGTAGACAGGAACTGGATAGAAGTCACATGTTAGTCAAAGGGAGGGAGGGTTCTTATCCGTACAGGGATTGGGATCCTAGCTCTGCTCATCATTTGCAATTGAAAAATGATGGGCTTAATAGGCGAAAAGAGAGGGAAAATCTTGACGGAGCATGGCAAAGGAGGGATGATGATCCTTATAGCAGAAGGATTAGAACTGAAGAACCAAGGAAGAGGGAACGTGGTGATGAAATGGGATCCAGGCACAGGAGTAAGGTTCGTGAAAGCGAGAGGAGTGACAAAGATGAATATATGCAGTCAAGAAAGCAGTTAGATAATGGTAGCTATAGGGTCTTTTATGATAAGGATGTTGGATCACGACCCAGGGAAAGAGAAGATAGTTTGAAGGGCAGGTATGAACATATAGATGATTACCATGGCAAGAGAAGGAAAGATGAGGAATACATGAGGAGGGACCAAATAGATAAAGAAGAACTTCTGCAGGGCCACAGAGATACTACCTCTCGCCGAAAGCGGGAAAGGGATGAAGTTTTAGATCAGCGTAAAAGAGATGACCAGCAGAAAGTCAGGGATAATCCTGATGACCACCACTCTGTCAGGCACAAAGATGAAAGCTGGTTGCCGAGGGAGAGAGGTGACAGACAAAGAGAAAGGGAGGAGTGGCATAGGCTTAAGCAATCCCATGAAGAAAATATACCCAAGAGGGAAAGAGATGATGGACGAGTTTCTGTAAGGGGTGGGCGTGCTGCAGAAGACAAAGCATGGGTAGGCCATGCTAGGGCAAAGGATGAGAACAAAGGCTCTGATAAGGAGTACCAATATAAAGAGACTGTGCGACACAGTGAGCAGTCTAAAAGAAGGGATAGGGTTGAGGAGGAAAGTTCACATCATAGGGGACGTGAGGATGCTCATGCACGGGGAAATCAAATGAATATTGATGAGAGGAGATCTGGCAAGGAGAGATCAAGTACTCGCAGTGAACGTGGTGATAGCCAAAAAGTCCAAGATAGAAAACATAAAGAGAATTCAAGAAGGAATAAAGAAACTGAAATTGCTGATATCAACACTTCAGTCACTTCCAAGAGACATCAAGAAGACCAAAGTGGTCGTAGCAAGGAGATGGTATGTACTGTTCTTGACATCAGGTCTCGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0042802 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fip1

Protein Analysis

1154

Amino Acids

129.42

Weight (kDa)

5.29

Isoelectric Point (pI)

61.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fip1 PF05182 344 - 386 2.2e-21 Fip1 motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
Acc36I ACCTGC 1 cut(s) 2688
AccB1I GGYRCC 2 cut(s) 88, 706
AccB7I CCANNNNNTGG 2 cut(s) 791, 863
AccI GTMKAC 3 cut(s) 1035, 1894, 2311
AccII CGCG 3 cut(s) 395, 978, 1941
AclI AACGTT 1 cut(s) 1399
AcoI YGGCCR 2 cut(s) 387, 1421
AcsI RAATTY 8 cut(s) 17, 313, 345, 925, 998, 1282, 1847, 3331
AcuI CTGAAG 3 cut(s) 1071, 2526, 3360
AcvI CACGTG 1 cut(s) 1799
AcyI GRCGYC 4 cut(s) 89, 267, 366, 707
AfaI GTAC 6 cut(s) 910, 946, 2369, 3122, 3277, 3436
AfiI CCNNNNNNNGG 9 cut(s) 716, 791, 863, 869, 956, 1293, 2553, 2821, 3085
AflII CTTAAG 1 cut(s) 2978
AflIII ACRYGT 1 cut(s) 2334
AhdI GACNNNNNGTC 1 cut(s) 3451
AjiI CACGTC 1 cut(s) 3206
AjnI CCWGG 9 cut(s) 772, 790, 811, 862, 949, 1072, 1291, 2546, 2669
AloI GAACNNNNNNTCC 4 cut(s) 2271, 2303, 2341, 2373
Alw21I GWGCWC 2 cut(s) 594, 1798
AlwNI CAGNNNCTG 3 cut(s) 1256, 1448, 2320
Ama87I CYCGRG 2 cut(s) 823, 2245
AoxI GGCC 8 cut(s) 387, 599, 1421, 1587, 1709, 2299, 2791, 3076
ApeKI GCWGC 8 cut(s) 631, 916, 1247, 1250, 1934, 2027, 2138, 3053
ApoI RAATTY 8 cut(s) 17, 313, 345, 925, 998, 1282, 1847, 3331
ArsI GACNNNNNNTTYG 1 cut(s) 34
Asp700I GAANNNNTTC 2 cut(s) 2223, 2781
AspLEI GCGC 3 cut(s) 91, 397, 709
AspS9I GGNCC 9 cut(s) 599, 695, 794, 809, 854, 1709, 1735, 2761, 2791
AsuC2I CCSGG 4 cut(s) 378, 425, 824, 825
AvaI CYCGRG 2 cut(s) 823, 2245
AvaII GGWCC 6 cut(s) 695, 794, 809, 854, 1735, 2761
BamHI GGATCC 3 cut(s) 1633, 2380, 2543
BanI GGYRCC 2 cut(s) 88, 706
BarI GAAGNNNNNNTAC 2 cut(s) 2985, 3017
BauI CACGAG 3 cut(s) 176, 2085, 3456
BbrPI CACGTG 1 cut(s) 1799
BbsI GAAGAC 5 cut(s) 12, 144, 2133, 3066, 3408
Bbv12I GWGCWC 2 cut(s) 594, 1798
BbvCI CCTCAGC 1 cut(s) 79
BbvI GCAGC 8 cut(s) 643, 903, 1259, 1262, 1921, 2014, 2125, 3040
BccI CCATC 9 cut(s) 520, 1883, 2048, 2114, 2198, 2300, 2411, 3017, 3421
BceAI ACGGC 4 cut(s) 55, 353, 460, 2286
BciT130I CCWGG 9 cut(s) 774, 792, 813, 864, 951, 1074, 1293, 2548, 2671
BciVI GTATCC 2 cut(s) 1517, 1776
BcnI CCSGG 4 cut(s) 378, 425, 824, 825
BfaI CTAG 7 cut(s) 149, 203, 246, 1824, 1988, 2385, 3084
BfmI CTRYAG 5 cut(s) 917, 1494, 2632, 2786, 3054
BfoI RGCGCY 2 cut(s) 92, 710
BfrI CTTAAG 1 cut(s) 2978
BfuAI ACCTGC 1 cut(s) 2688
BfuI GTATCC 2 cut(s) 1517, 1776
BglII AGATCT 4 cut(s) 25, 1228, 1805, 3254
BmcAI AGTACT 1 cut(s) 3277
Bme18I GGWCC 6 cut(s) 695, 794, 809, 854, 1735, 2761
BmeRI GACNNNNNGTC 1 cut(s) 3451
BmeT110I CYCGRG 2 cut(s) 823, 2245
BmgBI CACGTC 1 cut(s) 3206
BmgT120I GGNCC 9 cut(s) 599, 695, 794, 809, 854, 1709, 1735, 2761, 2791
BmiI GGNNCC 8 cut(s) 90, 708, 855, 1635, 1736, 2382, 2545, 2762
BmrI ACTGGG 2 cut(s) 748, 1740
BmsI GCATC 3 cut(s) 1435, 2262, 3202
BmuI ACTGGG 2 cut(s) 748, 1740
BpiI GAAGAC 5 cut(s) 12, 144, 2133, 3066, 3408
BpmI CTGGAG 4 cut(s) 834, 904, 1144, 1221
Bpu10I CCTNAGC 1 cut(s) 79
BpuEI CTTGAG 2 cut(s) 113, 1393
BpuMI CCSGG 4 cut(s) 378, 425, 824, 825
Bsa29I ATCGAT 1 cut(s) 117
BsaAI YACGTR 1 cut(s) 1799
BsaBI GATNNNNATC 3 cut(s) 1227, 2379, 2478
BsaHI GRCGYC 4 cut(s) 89, 267, 366, 707
BsaI GGTCTC 3 cut(s) 763, 880, 3459
BsaWI WCCGGW 2 cut(s) 350, 697
BsaXI ACNNNNNCTCC 6 cut(s) 190, 220, 602, 632, 2341, 2371
Bsc4I CCNNNNNNNGG 9 cut(s) 716, 791, 863, 869, 956, 1293, 2553, 2821, 3085
Bse118I RCCGGY 3 cut(s) 397, 601, 1419
Bse3DI GCAATG 1 cut(s) 547
Bse8I GATNNNNATC 3 cut(s) 1227, 2379, 2478
BseBI CCWGG 9 cut(s) 774, 792, 813, 864, 951, 1074, 1293, 2548, 2671
BseCI ATCGAT 1 cut(s) 117
BseJI GATNNNNATC 3 cut(s) 1227, 2379, 2478
BseLI CCNNNNNNNGG 9 cut(s) 716, 791, 863, 869, 956, 1293, 2553, 2821, 3085
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 3 cut(s) 93, 136, 1455
BseX3I CGGCCG 1 cut(s) 387
BseXI GCAGC 8 cut(s) 643, 903, 1259, 1262, 1921, 2014, 2125, 3040
BseYI CCCAGC 2 cut(s) 66, 154
Bsh1236I CGCG 3 cut(s) 395, 978, 1941
Bsh1285I CGRYCG 2 cut(s) 117, 390
BshFI GGCC 8 cut(s) 389, 601, 1423, 1589, 1711, 2301, 2793, 3078
BshNI GGYRCC 2 cut(s) 88, 706
BshVI ATCGAT 1 cut(s) 117
BsiEI CGRYCG 2 cut(s) 117, 390
BsiHKAI GWGCWC 2 cut(s) 594, 1798
BsiHKCI CYCGRG 2 cut(s) 823, 2245
BslFI GGGAC 6 cut(s) 372, 479, 806, 1748, 2774, 3216
BslI CCNNNNNNNGG 9 cut(s) 716, 791, 863, 869, 956, 1293, 2553, 2821, 3085
BsmBI CGTCTC 3 cut(s) 435, 1560, 1927
BsmFI GGGAC 6 cut(s) 372, 479, 806, 1748, 2774, 3216
BsmI GAATGC 1 cut(s) 1437
BsnI GGCC 8 cut(s) 389, 601, 1423, 1589, 1711, 2301, 2793, 3078
Bso31I GGTCTC 3 cut(s) 763, 880, 3459
BsoBI CYCGRG 2 cut(s) 823, 2245
Bsp1286I GDGCHC 2 cut(s) 594, 1798
Bsp19I CCATGG 1 cut(s) 2722
BspANI GGCC 8 cut(s) 389, 601, 1423, 1589, 1711, 2301, 2793, 3078
BspCNI CTCAG 3 cut(s) 92, 135, 1454
BspDI ATCGAT 1 cut(s) 117
BspFNI CGCG 3 cut(s) 395, 978, 1941
BspLI GGNNCC 8 cut(s) 90, 708, 855, 1635, 1736, 2382, 2545, 2762
BspMAI CTGCAG 4 cut(s) 921, 1498, 2790, 3058
BspMI ACCTGC 1 cut(s) 2688
BspQI GCTCTTC 2 cut(s) 1360, 1786
BspT107I GGYRCC 2 cut(s) 88, 706
BspTI CTTAAG 1 cut(s) 2978
BspTNI GGTCTC 3 cut(s) 763, 880, 3459
BsrDI GCAATG 1 cut(s) 547
BsrFI RCCGGY 3 cut(s) 397, 601, 1419
BssAI RCCGGY 3 cut(s) 397, 601, 1419
BssNI GRCGYC 4 cut(s) 89, 267, 366, 707
BssSI CACGAG 3 cut(s) 176, 2085, 3456
BssT1I CCWWGG 4 cut(s) 1061, 2133, 2513, 2722
Bst2BI CACGAG 3 cut(s) 176, 2085, 3456
Bst2UI CCWGG 9 cut(s) 774, 792, 813, 864, 951, 1074, 1293, 2548, 2671
Bst4CI ACNGT 9 cut(s) 110, 412, 470, 587, 1046, 1859, 3139, 3149, 3439
Bst6I CTCTTC 6 cut(s) 433, 543, 618, 1360, 1786, 2513
BstACI GRCGYC 4 cut(s) 89, 267, 366, 707
BstAFI CTTAAG 1 cut(s) 2978
BstBAI YACGTR 1 cut(s) 1799
BstC8I GCNNGC 6 cut(s) 603, 1339, 1343, 1691, 1939, 3051
BstDEI CTNAG 3 cut(s) 79, 122, 1441
BstDSI CCRYGG 1 cut(s) 2722
BstEII GGTNACC 1 cut(s) 1967
BstENI CCTNNNNNAGG 1 cut(s) 867
BstFNI CGCG 3 cut(s) 395, 978, 1941
BstH2I RGCGCY 2 cut(s) 92, 710
BstHHI GCGC 3 cut(s) 91, 397, 709
BstMCI CGRYCG 2 cut(s) 117, 390
BstMWI GCNNNNNNNGC 6 cut(s) 100, 453, 598, 690, 1150, 2428
BstNI CCWGG 9 cut(s) 774, 792, 813, 864, 951, 1074, 1293, 2548, 2671
BstNSI RCATGY 2 cut(s) 1724, 2338
BstPI GGTNACC 1 cut(s) 1967
BstSFI CTRYAG 5 cut(s) 917, 1494, 2632, 2786, 3054
BstUI CGCG 3 cut(s) 395, 978, 1941
BstV1I GCAGC 8 cut(s) 643, 903, 1259, 1262, 1921, 2014, 2125, 3040
BstV2I GAAGAC 5 cut(s) 12, 144, 2133, 3066, 3408
BstX2I RGATCY 9 cut(s) 25, 144, 1228, 1331, 1633, 1805, 2380, 2543, 3254
BstXI CCANNNNNNTGG 1 cut(s) 1724
BstYI RGATCY 9 cut(s) 25, 144, 1228, 1331, 1633, 1805, 2380, 2543, 3254
BstZI CGGCCG 1 cut(s) 387
Bsu15I ATCGAT 1 cut(s) 117
BsuI GTATCC 2 cut(s) 1517, 1776
BsuRI GGCC 8 cut(s) 389, 601, 1423, 1589, 1711, 2301, 2793, 3078
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 2722
BtgZI GCGATG 1 cut(s) 471
BtrI CACGTC 1 cut(s) 3206
BtsI GCAGTG 1 cut(s) 3289
BtsIMutI CAGTG 5 cut(s) 475, 592, 1881, 3154, 3289
BveI ACCTGC 1 cut(s) 2688
Cac8I GCNNGC 6 cut(s) 603, 1339, 1343, 1691, 1939, 3051
CaiI CAGNNNCTG 3 cut(s) 1256, 1448, 2320
CfoI GCGC 3 cut(s) 91, 397, 709
Cfr10I RCCGGY 3 cut(s) 397, 601, 1419
Cfr13I GGNCC 9 cut(s) 599, 695, 794, 809, 854, 1709, 1735, 2761, 2791
Cfr9I CCCGGG 1 cut(s) 823
ClaI ATCGAT 1 cut(s) 117
CpoI CGGWCCG 1 cut(s) 695
CseI GACGC 2 cut(s) 374, 1334
CsiI ACCWGGT 3 cut(s) 772, 862, 949
Csp6I GTAC 6 cut(s) 909, 945, 2368, 3121, 3276, 3435
CspCI CAANNNNNGTGG 4 cut(s) 952, 987, 1359, 1394
CspI CGGWCCG 1 cut(s) 695
CviQI GTAC 6 cut(s) 909, 945, 2368, 3121, 3276, 3435
DdeI CTNAG 3 cut(s) 79, 122, 1441
DinI GGCGCC 2 cut(s) 90, 708
DriI GACNNNNNGTC 1 cut(s) 3451
EaeI YGGCCR 2 cut(s) 387, 1421
EagI CGGCCG 1 cut(s) 387
Eam1104I CTCTTC 6 cut(s) 433, 543, 618, 1360, 1786, 2513
Eam1105I GACNNNNNGTC 1 cut(s) 3451
EarI CTCTTC 6 cut(s) 433, 543, 618, 1360, 1786, 2513
EciI GGCGGA 6 cut(s) 252, 465, 596, 620, 659, 699
EclXI CGGCCG 1 cut(s) 387
Eco130I CCWWGG 4 cut(s) 1061, 2133, 2513, 2722
Eco147I AGGCCT 1 cut(s) 1589
Eco31I GGTCTC 3 cut(s) 763, 880, 3459
Eco32I GATATC 2 cut(s) 322, 3367
Eco47I GGWCC 6 cut(s) 695, 794, 809, 854, 1735, 2761
Eco52I CGGCCG 1 cut(s) 387
Eco57I CTGAAG 3 cut(s) 1071, 2526, 3360
Eco72I CACGTG 1 cut(s) 1799
Eco88I CYCGRG 2 cut(s) 823, 2245
Eco91I GGTNACC 1 cut(s) 1967
EcoNI CCTNNNNNAGG 1 cut(s) 867
EcoO65I GGTNACC 1 cut(s) 1967
EcoRI GAATTC 3 cut(s) 17, 998, 3331
EcoRII CCWGG 9 cut(s) 772, 790, 811, 862, 949, 1072, 1291, 2546, 2669
EcoRV GATATC 2 cut(s) 322, 3367
EcoT14I CCWWGG 4 cut(s) 1061, 2133, 2513, 2722
EcoT22I ATGCAT 1 cut(s) 1266
EgeI GGCGCC 2 cut(s) 90, 708
EheI GGCGCC 2 cut(s) 90, 708
ErhI CCWWGG 4 cut(s) 1061, 2133, 2513, 2722
Esp3I CGTCTC 3 cut(s) 435, 1560, 1927
FalI AAGNNNNNCTT 4 cut(s) 1577, 1609, 2766, 2798
FaqI GGGAC 6 cut(s) 372, 479, 806, 1748, 2774, 3216
FauI CCCGC 2 cut(s) 1946, 2814
FblI GTMKAC 3 cut(s) 1035, 1894, 2311
FspBI CTAG 7 cut(s) 149, 203, 246, 1824, 1988, 2385, 3084
GlaI GCGC 3 cut(s) 90, 396, 708
GsaI CCCAGC 2 cut(s) 70, 158
GsuI CTGGAG 4 cut(s) 834, 904, 1144, 1221
HaeII RGCGCY 2 cut(s) 92, 710
HaeIII GGCC 8 cut(s) 389, 601, 1423, 1589, 1711, 2301, 2793, 3078
HgaI GACGC 2 cut(s) 374, 1334
HhaI GCGC 3 cut(s) 91, 397, 709
Hin1I GRCGYC 4 cut(s) 89, 267, 366, 707
Hin6I GCGC 3 cut(s) 89, 395, 707
HinP1I GCGC 3 cut(s) 89, 395, 707
HincII GTYRAC 2 cut(s) 1036, 2092
HindII GTYRAC 2 cut(s) 1036, 2092
HindIII AAGCTT 1 cut(s) 1687
Hpy188I TCNGA 8 cut(s) 23, 114, 292, 1090, 1303, 1444, 2193, 3112
Hpy99I CGWCG 4 cut(s) 14, 41, 563, 566
HpyCH4III ACNGT 9 cut(s) 110, 412, 470, 587, 1046, 1859, 3139, 3149, 3439
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 453, 598, 690, 1150, 2428
HpyF3I CTNAG 3 cut(s) 79, 122, 1441
Hsp92I GRCGYC 4 cut(s) 89, 267, 366, 707
HspAI GCGC 3 cut(s) 89, 395, 707
KasI GGCGCC 2 cut(s) 88, 706
KroI GCCGGC 1 cut(s) 601
KroNI GCCGGC 1 cut(s) 603
LguI GCTCTTC 2 cut(s) 1360, 1786
LmnI GCTCC 8 cut(s) 88, 108, 163, 176, 211, 610, 1158, 2456
Lsp1109I GCAGC 8 cut(s) 643, 903, 1259, 1262, 1921, 2014, 2125, 3040
LweI GCATC 3 cut(s) 1435, 2262, 3202
MabI ACCWGGT 3 cut(s) 772, 862, 949
MaeI CTAG 7 cut(s) 149, 203, 246, 1824, 1988, 2385, 3084
MfeI CAATTG 1 cut(s) 2405
MflI RGATCY 9 cut(s) 25, 144, 1228, 1331, 1633, 1805, 2380, 2543, 3254
MhlI GDGCHC 2 cut(s) 594, 1798
Mly113I GGCGCC 2 cut(s) 89, 707
MmeI TCCRAC 5 cut(s) 881, 1311, 1382, 1741, 2638
Mph1103I ATGCAT 1 cut(s) 1266
MroNI GCCGGC 1 cut(s) 601
MroXI GAANNNNTTC 2 cut(s) 2223, 2781
MseI TTAA 4 cut(s) 759, 899, 2424, 2979
MslI CAYNNNNRTG 3 cut(s) 2268, 2712, 2913
MspA1I CMGCKG 2 cut(s) 916, 1144
MspCI CTTAAG 1 cut(s) 2978
MunI CAATTG 1 cut(s) 2405
Mva1269I GAATGC 1 cut(s) 1437
MvaI CCWGG 9 cut(s) 774, 792, 813, 864, 951, 1074, 1293, 2548, 2671
MvnI CGCG 3 cut(s) 395, 978, 1941
MwoI GCNNNNNNNGC 6 cut(s) 100, 453, 598, 690, 1150, 2428
NaeI GCCGGC 1 cut(s) 603
NarI GGCGCC 2 cut(s) 89, 707
NciI CCSGG 4 cut(s) 378, 425, 824, 825
NcoI CCATGG 1 cut(s) 2722
NgoMIV GCCGGC 1 cut(s) 601
NlaIV GGNNCC 8 cut(s) 90, 708, 855, 1635, 1736, 2382, 2545, 2762
NmeAIII GCCGAG 1 cut(s) 2955
NsiI ATGCAT 1 cut(s) 1266
NspI RCATGY 2 cut(s) 1724, 2338
PasI CCCWGGG 2 cut(s) 1292, 2670
PceI AGGCCT 1 cut(s) 1589
PciI ACATGT 1 cut(s) 2334
PciSI GCTCTTC 2 cut(s) 1360, 1786
PcsI WCGNNNNNNNCGW 1 cut(s) 3286
PctI GAATGC 1 cut(s) 1437
PdiI GCCGGC 1 cut(s) 603
PdmI GAANNNNTTC 2 cut(s) 2223, 2781
PfeI GAWTC 7 cut(s) 212, 230, 287, 373, 431, 1487, 2286
PflFI GACNNNGTC 1 cut(s) 1899
PflMI CCANNNNNTGG 2 cut(s) 791, 863
PfoI TCCNGGA 1 cut(s) 811
Ple19I CGATCG 1 cut(s) 117
PluTI GGCGCC 2 cut(s) 92, 710
PmaCI CACGTG 1 cut(s) 1799
PmlI CACGTG 1 cut(s) 1799
Ppu21I YACGTR 1 cut(s) 1799
PscI ACATGT 1 cut(s) 2334
Psp1406I AACGTT 1 cut(s) 1399
Psp6I CCWGG 9 cut(s) 772, 790, 811, 862, 949, 1072, 1291, 2546, 2669
PspCI CACGTG 1 cut(s) 1799
PspEI GGTNACC 1 cut(s) 1967
PspFI CCCAGC 2 cut(s) 66, 154
PspGI CCWGG 9 cut(s) 772, 790, 811, 862, 949, 1072, 1291, 2546, 2669
PspN4I GGNNCC 8 cut(s) 90, 708, 855, 1635, 1736, 2382, 2545, 2762
PspPI GGNCC 9 cut(s) 599, 695, 794, 809, 854, 1709, 1735, 2761, 2791
PsrI GAACNNNNNNTAC 2 cut(s) 3423, 3455
PstI CTGCAG 4 cut(s) 921, 1498, 2790, 3058
PstNI CAGNNNCTG 3 cut(s) 1256, 1448, 2320
PsuI RGATCY 9 cut(s) 25, 144, 1228, 1331, 1633, 1805, 2380, 2543, 3254
PsyI GACNNNGTC 1 cut(s) 1899
PvuI CGATCG 1 cut(s) 117
PvuII CAGCTG 1 cut(s) 1144
RsaI GTAC 6 cut(s) 910, 946, 2369, 3122, 3277, 3436
RsaNI GTAC 6 cut(s) 909, 945, 2368, 3121, 3276, 3435
RseI CAYNNNNRTG 3 cut(s) 2268, 2712, 2913
Rsr2I CGGWCCG 1 cut(s) 695
RsrII CGGWCCG 1 cut(s) 695
SalI GTCGAC 1 cut(s) 1034
SapI GCTCTTC 2 cut(s) 1360, 1786
SaqAI TTAA 4 cut(s) 759, 899, 2424, 2979
Sau96I GGNCC 9 cut(s) 599, 695, 794, 809, 854, 1709, 1735, 2761, 2791
ScaI AGTACT 1 cut(s) 3277
SduI GDGCHC 2 cut(s) 594, 1798
SexAI ACCWGGT 3 cut(s) 772, 862, 949
SfaNI GCATC 3 cut(s) 1435, 2262, 3202
SfcI CTRYAG 5 cut(s) 917, 1494, 2632, 2786, 3054
SfoI GGCGCC 2 cut(s) 90, 708
SgrAI CRCCGGYG 1 cut(s) 397
SinI GGWCC 6 cut(s) 695, 794, 809, 854, 1735, 2761
SmaI CCCGGG 1 cut(s) 825
SmiMI CAYNNNNRTG 3 cut(s) 2268, 2712, 2913
SmlI CTYRAG 3 cut(s) 128, 1408, 2978
SmoI CTYRAG 3 cut(s) 128, 1408, 2978
SseBI AGGCCT 1 cut(s) 1589
SspDI GGCGCC 2 cut(s) 88, 706
SspI AATATT 1 cut(s) 3241
SspMI CTAG 7 cut(s) 149, 203, 246, 1824, 1988, 2385, 3084
StuI AGGCCT 1 cut(s) 1589
StyI CCWWGG 4 cut(s) 1061, 2133, 2513, 2722
TaaI ACNGT 9 cut(s) 110, 412, 470, 587, 1046, 1859, 3139, 3149, 3439
TaqI TCGA 9 cut(s) 117, 215, 318, 324, 434, 868, 1035, 1624, 1953
TaqII GACCGA 1 cut(s) 149
TatI WGTACW 2 cut(s) 3275, 3434
TauI GCSGC 7 cut(s) 94, 97, 227, 392, 395, 450, 644
TfiI GAWTC 7 cut(s) 212, 230, 287, 373, 431, 1487, 2286
Tru1I TTAA 4 cut(s) 759, 899, 2424, 2979
Tru9I TTAA 4 cut(s) 759, 899, 2424, 2979
TscAI CASTG 5 cut(s) 475, 592, 1881, 3154, 3289
TseI GCWGC 8 cut(s) 631, 916, 1247, 1250, 1934, 2027, 2138, 3053
TspGWI ACGGA 7 cut(s) 314, 1175, 1530, 1688, 1893, 2355, 2469
TspMI CCCGGG 1 cut(s) 823
TspRI CASTG 5 cut(s) 475, 592, 1881, 3154, 3289
Tth111I GACNNNGTC 1 cut(s) 1899
Van91I CCANNNNNTGG 2 cut(s) 791, 863
Vha464I CTTAAG 1 cut(s) 2978
VpaK11BI GGWCC 6 cut(s) 695, 794, 809, 854, 1735, 2761
XagI CCTNNNNNAGG 1 cut(s) 867
XapI RAATTY 8 cut(s) 17, 313, 345, 925, 998, 1282, 1847, 3331
XceI RCATGY 2 cut(s) 1724, 2338
XmaI CCCGGG 1 cut(s) 823
XmiI GTMKAC 3 cut(s) 1035, 1894, 2311
XmnI GAANNNNTTC 2 cut(s) 2223, 2781
XspI CTAG 7 cut(s) 149, 203, 246, 1824, 1988, 2385, 3084
ZraI GACGTC 1 cut(s) 268
ZrmI AGTACT 1 cut(s) 3277
Zsp2I ATGCAT 1 cut(s) 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.