MD02G1014300.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012553
Physical Location & Seq
Reverse (-)
933360 .. 936621
3262 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1014300.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGTGCCTGATAGACGCAGAACAGCTCGGTCAAGAAATGGGGAACATGGAGAATAATAACGACATCGATTCCAACGACAATAACAACAAGCAGTGGTCTCTGCAATTAGACCACCGGACGGAGGAGGAGAATTTGGACCGGGAAACTCCTACTGTGATGAGCTCAAGTCCTCAGAAGAACATGATAAATTCGTTTCAACTTGAAAGCATAGGCGAGGATACAGTGATTGCAGACAAAAAGCAGAGCCTGTTGAACTTTGTTCCTGCCCTTCGGTCAGGAGAGTGGTCTGATATTGGTGGTCGAACCCATATGGAGGATACTCATATATGCATTGGTGACTTAGCTAAGCAGTTTGGTTATAATCTACTCGGTGGGGAAGCTATCTCCTTCTATGGTGTATTTGATGGCCATGGAGGAAAGGATGCAGCACACTTTGTTCGTGACAATTTACCAAGAGTCATTGTTGAGGATGCTGACTTTCCTTTAGAACTTGAAAAGGTGATTACAAGGTCATTTATGGAGACAGATGCTGCATTTGCAAAGACGTGTTCTCTTGAGGCTCTTGTTTCTGGCACAACTGCACTCACTGCAATGATATTTGGGAGATCTTTGCTTGTGGCAAATGCCGGAGATTGCCGGGCTGTATTATCGCGATGTGGAGTGGCTATAGAAATGTCAAGGGATCATAGGCCATGTTGTACAAAAGAAAGAACGCGGATTGAGTCCTTGGGTGGATATATTGATGATGGTTTTCTAAATGGTGAGTTAGGTGTGACCCGTGCATTAGGTAATTGGCATCTTGAAGGAATGAAGGACGTGGGTGAAAGGGGCGGACCCTTGAGCGCTGAGCCAGAACTTAAACTGATGACACTGAGCAAAGATGATGAGTTTTTGATCATTGGCAGTGATGGGATATGGGATGTGTTCACCAGCCGAAATGCTGTTGATTTCGCTCGAAGGAGACTGCAAGAGCACAATGATGTGAAGTTGTGCTGCAAGCAAATAGTAGAGGAGGCAATAAAGCGGGGAGCAACGGACAATTTGACGCTTGTCATGGTAAGTTTCCACTTGGAGCCACCTCCACATGTGGTGGTAGAGAGACCTCGAGTCAGACGAAGCATTTCTGCCGAGGGACTTCAGAATCTCAAATTCTTCTTGGACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

389

Amino Acids

43.16

Weight (kDa)

4.96

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 100 - 347 5.6e-64 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
AccII CGCG 2 cut(s) 652, 715
AciI CCGC 3 cut(s) 715, 831, 1024
AclWI GGATC 1 cut(s) 690
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 3 cut(s) 130, 187, 1148
AcuI CTGAAG 1 cut(s) 1121
AfaI GTAC 1 cut(s) 700
AfeI AGCGCT 1 cut(s) 844
AfiI CCNNNNNNNGG 3 cut(s) 118, 121, 313
AflIII ACRYGT 2 cut(s) 545, 1084
AgsI TTSAA 5 cut(s) 197, 203, 253, 494, 803
AhdI GACNNNNNGTC 1 cut(s) 1106
AjiI CACGTC 2 cut(s) 546, 817
AluBI AGCT 4 cut(s) 25, 162, 344, 380
AluI AGCT 4 cut(s) 25, 162, 344, 380
Alw21I GWGCWC 2 cut(s) 164, 975
Alw26I GTCTC 4 cut(s) 102, 515, 955, 1093
AlwI GGATC 1 cut(s) 690
AlwNI CAGNNNCTG 2 cut(s) 247, 530
Ama87I CYCGRG 1 cut(s) 1104
Aor51HI AGCGCT 1 cut(s) 844
AoxI GGCC 2 cut(s) 406, 689
ApeKI GCWGC 3 cut(s) 425, 530, 993
ApoI RAATTY 3 cut(s) 130, 187, 1148
Asp700I GAANNNNTTC 1 cut(s) 1120
AspLEI GCGC 1 cut(s) 845
AspS9I GGNCC 2 cut(s) 136, 833
AsuC2I CCSGG 2 cut(s) 140, 638
AsuHPI GGTGA 5 cut(s) 347, 511, 773, 833, 919
AvaI CYCGRG 1 cut(s) 1104
AvaII GGWCC 2 cut(s) 136, 833
BalI TGGCCA 1 cut(s) 408
BanII GRGCYC 1 cut(s) 164
Bbv12I GWGCWC 2 cut(s) 164, 975
BbvI GCAGC 3 cut(s) 437, 517, 980
BccI CCATC 3 cut(s) 398, 740, 902
BciVI GTATCC 2 cut(s) 211, 310
BclI TGATCA 1 cut(s) 894
BcnI CCSGG 2 cut(s) 140, 638
BcoDI GTCTC 4 cut(s) 102, 515, 955, 1093
BfmI CTRYAG 1 cut(s) 666
BfoI RGCGCY 1 cut(s) 846
BfuI GTATCC 2 cut(s) 211, 310
BglII AGATCT 1 cut(s) 605
BisI GCNGC 3 cut(s) 426, 531, 994
BlpI GCTNAGC 2 cut(s) 345, 846
BlsI GCNGC 3 cut(s) 427, 532, 995
Bme1390I CCNGG 2 cut(s) 140, 638
Bme18I GGWCC 2 cut(s) 136, 833
BmeRI GACNNNNNGTC 1 cut(s) 1106
BmeT110I CYCGRG 1 cut(s) 1104
BmgBI CACGTC 2 cut(s) 546, 817
BmgT120I GGNCC 2 cut(s) 136, 833
BmiI GGNNCC 2 cut(s) 835, 1074
BmrFI CCNGG 2 cut(s) 140, 638
BmsI GCATC 4 cut(s) 412, 460, 517, 805
BoxI GACNNNNGTC 1 cut(s) 1049
Bpu1102I GCTNAGC 2 cut(s) 345, 846
BpuEI CTTGAG 3 cut(s) 148, 575, 859
BpuMI CCSGG 2 cut(s) 140, 638
Bsa29I ATCGAT 1 cut(s) 66
BsaI GGTCTC 2 cut(s) 102, 1093
BsaJI CCNNGG 3 cut(s) 409, 726, 1128
BsaWI WCCGGW 1 cut(s) 114
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 121, 313
Bse3DI GCAATG 1 cut(s) 597
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 3 cut(s) 409, 726, 1128
BseGI GGATG 3 cut(s) 427, 475, 925
BseLI CCNNNNNNNGG 3 cut(s) 118, 121, 313
BseMI GCAATG 1 cut(s) 597
BseMII CTCAG 3 cut(s) 185, 837, 863
BseRI GAGGAG 3 cut(s) 137, 140, 1025
BseXI GCAGC 3 cut(s) 437, 517, 980
BsgI GTGCAG 1 cut(s) 564
Bsh1236I CGCG 2 cut(s) 652, 715
BshFI GGCC 2 cut(s) 408, 691
BshVI ATCGAT 1 cut(s) 66
BsiHKAI GWGCWC 2 cut(s) 164, 975
BsiHKCI CYCGRG 1 cut(s) 1104
BsiSI CCGG 4 cut(s) 115, 139, 627, 637
BslFI GGGAC 1 cut(s) 1146
BslI CCNNNNNNNGG 3 cut(s) 118, 121, 313
BsmAI GTCTC 4 cut(s) 102, 515, 955, 1093
BsmFI GGGAC 1 cut(s) 1146
BsnI GGCC 2 cut(s) 408, 691
Bso31I GGTCTC 2 cut(s) 102, 1093
BsoBI CYCGRG 1 cut(s) 1104
Bsp1286I GDGCHC 2 cut(s) 164, 975
Bsp1407I TGTACA 1 cut(s) 698
Bsp143I GATC 3 cut(s) 605, 682, 894
Bsp1720I GCTNAGC 2 cut(s) 345, 846
Bsp19I CCATGG 1 cut(s) 409
Bsp68I TCGCGA 1 cut(s) 652
BspACI CCGC 3 cut(s) 715, 831, 1024
BspANI GGCC 2 cut(s) 408, 691
BspCNI CTCAG 3 cut(s) 184, 838, 864
BspDI ATCGAT 1 cut(s) 66
BspFNI CGCG 2 cut(s) 652, 715
BspLI GGNNCC 2 cut(s) 835, 1074
BspPI GGATC 1 cut(s) 690
BspTNI GGTCTC 2 cut(s) 102, 1093
BsrDI GCAATG 1 cut(s) 597
BsrGI TGTACA 1 cut(s) 698
BssECI CCNNGG 3 cut(s) 409, 726, 1128
BssMI GATC 3 cut(s) 605, 682, 894
BssT1I CCWWGG 2 cut(s) 409, 726
Bst4CI ACNGT 2 cut(s) 154, 223
BstAPI GCANNNNNTGC 1 cut(s) 587
BstAUI TGTACA 1 cut(s) 698
BstC8I GCNNGC 1 cut(s) 998
BstDEI CTNAG 5 cut(s) 171, 340, 345, 846, 872
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 3 cut(s) 427, 475, 925
BstFNI CGCG 2 cut(s) 652, 715
BstH2I RGCGCY 1 cut(s) 846
BstHHI GCGC 1 cut(s) 845
BstKTI GATC 3 cut(s) 608, 685, 897
BstMAI GTCTC 4 cut(s) 102, 515, 955, 1093
BstMBI GATC 3 cut(s) 605, 682, 894
BstMWI GCNNNNNNNGC 2 cut(s) 536, 587
BstNSI RCATGY 1 cut(s) 1088
BstPAI GACNNNNGTC 1 cut(s) 1049
BstSCI CCNGG 2 cut(s) 138, 636
BstSFI CTRYAG 1 cut(s) 666
BstUI CGCG 2 cut(s) 652, 715
BstV1I GCAGC 3 cut(s) 437, 517, 980
BstX2I RGATCY 1 cut(s) 605
BstYI RGATCY 1 cut(s) 605
Bsu15I ATCGAT 1 cut(s) 66
BsuI GTATCC 2 cut(s) 211, 310
BsuRI GGCC 2 cut(s) 408, 691
BsuTUI ATCGAT 1 cut(s) 66
BtgI CCRYGG 1 cut(s) 409
BtgZI GCGATG 1 cut(s) 667
BtrI CACGTC 2 cut(s) 546, 817
BtsCI GGATG 3 cut(s) 427, 475, 925
BtsI GCAGTG 3 cut(s) 98, 585, 910
BtsIMutI CAGTG 5 cut(s) 98, 228, 585, 869, 910
BtuMI TCGCGA 1 cut(s) 652
Cac8I GCNNGC 1 cut(s) 998
CaiI CAGNNNCTG 2 cut(s) 247, 530
CfoI GCGC 1 cut(s) 845
Cfr13I GGNCC 2 cut(s) 136, 833
ClaI ATCGAT 1 cut(s) 66
CseI GACGC 2 cut(s) 23, 1054
Csp6I GTAC 1 cut(s) 699
CviAII CATG 6 cut(s) 46, 181, 410, 693, 1054, 1085
CviQI GTAC 1 cut(s) 699
DdeI CTNAG 5 cut(s) 171, 340, 345, 846, 872
DpnI GATC 3 cut(s) 607, 684, 896
DpnII GATC 3 cut(s) 605, 682, 894
DriI GACNNNNNGTC 1 cut(s) 1106
EaeI YGGCCR 1 cut(s) 406
Eam1105I GACNNNNNGTC 1 cut(s) 1106
EciI GGCGGA 1 cut(s) 846
Ecl136II GAGCTC 1 cut(s) 162
Eco130I CCWWGG 2 cut(s) 409, 726
Eco24I GRGCYC 1 cut(s) 164
Eco31I GGTCTC 2 cut(s) 102, 1093
Eco47I GGWCC 2 cut(s) 136, 833
Eco47III AGCGCT 1 cut(s) 844
Eco53kI GAGCTC 1 cut(s) 162
Eco57I CTGAAG 1 cut(s) 1121
Eco88I CYCGRG 1 cut(s) 1104
EcoICRI GAGCTC 1 cut(s) 162
EcoT14I CCWWGG 2 cut(s) 409, 726
EcoT22I ATGCAT 1 cut(s) 332
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 2 cut(s) 409, 726
FaeI CATG 6 cut(s) 49, 184, 413, 696, 1057, 1088
FaqI GGGAC 1 cut(s) 1146
FatI CATG 6 cut(s) 45, 180, 409, 692, 1053, 1084
FauI CCCGC 1 cut(s) 1017
FauNDI CATATG 1 cut(s) 309
FbaI TGATCA 1 cut(s) 894
Fnu4HI GCNGC 3 cut(s) 426, 531, 994
FokI GGATG 3 cut(s) 434, 482, 932
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 3 cut(s) 426, 531, 994
GlaI GCGC 1 cut(s) 844
GluI GCNGC 3 cut(s) 426, 531, 994
HaeII RGCGCY 1 cut(s) 846
HaeIII GGCC 2 cut(s) 408, 691
HapII CCGG 4 cut(s) 115, 139, 627, 637
HgaI GACGC 2 cut(s) 23, 1054
HhaI GCGC 1 cut(s) 845
Hin1II CATG 6 cut(s) 49, 184, 413, 696, 1057, 1088
Hin6I GCGC 1 cut(s) 843
HinP1I GCGC 1 cut(s) 843
HinfI GANTC 5 cut(s) 68, 456, 722, 1107, 1141
HpaII CCGG 4 cut(s) 115, 139, 627, 637
HphI GGTGA 5 cut(s) 347, 511, 773, 833, 919
Hpy166II GTNNAC 1 cut(s) 927
Hpy188I TCNGA 4 cut(s) 174, 289, 1112, 1140
Hpy188III TCNNGA 6 cut(s) 32, 276, 440, 554, 651, 800
Hpy8I GTNNAC 1 cut(s) 927
HpyAV CCTTC 5 cut(s) 278, 397, 797, 805, 951
HpyCH4III ACNGT 2 cut(s) 154, 223
HpyCH4IV ACGT 2 cut(s) 545, 816
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 587
HpyF3I CTNAG 5 cut(s) 171, 340, 345, 846, 872
HpySE526I ACGT 2 cut(s) 545, 816
Hsp92II CATG 6 cut(s) 49, 184, 413, 696, 1057, 1088
HspAI GCGC 1 cut(s) 843
Ksp22I TGATCA 1 cut(s) 894
Kzo9I GATC 3 cut(s) 605, 682, 894
LmnI GCTCC 2 cut(s) 1028, 1072
Lsp1109I GCAGC 3 cut(s) 437, 517, 980
LweI GCATC 4 cut(s) 412, 460, 517, 805
MaeII ACGT 2 cut(s) 545, 816
MaeIII GTNAC 3 cut(s) 335, 440, 772
MalI GATC 3 cut(s) 607, 684, 896
MboI GATC 3 cut(s) 605, 682, 894
MboII GAAGA 2 cut(s) 187, 1144
MflI RGATCY 1 cut(s) 605
MhlI GDGCHC 2 cut(s) 164, 975
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 7 cut(s) 104, 130, 187, 445, 790, 1039, 1148
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 3 cut(s) 465, 731, 1116
MmeI TCCRAC 1 cut(s) 96
Mox20I TGGCCA 1 cut(s) 408
Mph1103I ATGCAT 1 cut(s) 332
MroXI GAANNNNTTC 1 cut(s) 1120
MscI TGGCCA 1 cut(s) 408
MseI TTAA 1 cut(s) 858
MslI CAYNNNNRTG 2 cut(s) 590, 978
Msp20I TGGCCA 1 cut(s) 408
MspI CCGG 4 cut(s) 115, 139, 627, 637
MspR9I CCNGG 2 cut(s) 140, 638
MvnI CGCG 2 cut(s) 652, 715
MwoI GCNNNNNNNGC 2 cut(s) 536, 587
NciI CCSGG 2 cut(s) 140, 638
NcoI CCATGG 1 cut(s) 409
NdeI CATATG 1 cut(s) 309
NdeII GATC 3 cut(s) 605, 682, 894
NlaIII CATG 6 cut(s) 49, 184, 413, 696, 1057, 1088
NlaIV GGNNCC 2 cut(s) 835, 1074
NmeAIII GCCGAG 1 cut(s) 1153
NmuCI GTSAC 3 cut(s) 335, 440, 772
NruI TCGCGA 1 cut(s) 652
NsiI ATGCAT 1 cut(s) 332
NspI RCATGY 1 cut(s) 1088
PaeR7I CTCGAG 1 cut(s) 1104
PciI ACATGT 1 cut(s) 1084
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 1111
PdmI GAANNNNTTC 1 cut(s) 1120
PfeI GAWTC 2 cut(s) 68, 1141
PkrI GCNGC 3 cut(s) 427, 532, 995
PleI GAGTC 3 cut(s) 464, 730, 1115
PpsI GAGTC 3 cut(s) 464, 730, 1115
PscI ACATGT 1 cut(s) 1084
PshAI GACNNNNGTC 1 cut(s) 1049
PsiI TTATAA 1 cut(s) 360
Psp124BI GAGCTC 1 cut(s) 164
PspN4I GGNNCC 2 cut(s) 835, 1074
PspPI GGNCC 2 cut(s) 136, 833
PspXI VCTCGAGB 1 cut(s) 1104
PstNI CAGNNNCTG 2 cut(s) 247, 530
PsuI RGATCY 1 cut(s) 605
RruI TCGCGA 1 cut(s) 652
RsaI GTAC 1 cut(s) 700
RsaNI GTAC 1 cut(s) 699
RseI CAYNNNNRTG 2 cut(s) 590, 978
SacI GAGCTC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 858
SatI GCNGC 3 cut(s) 426, 531, 994
Sau3AI GATC 3 cut(s) 605, 682, 894
Sau96I GGNCC 2 cut(s) 136, 833
SchI GAGTC 3 cut(s) 465, 731, 1116
ScrFI CCNGG 2 cut(s) 140, 638
SduI GDGCHC 2 cut(s) 164, 975
SfaNI GCATC 4 cut(s) 412, 460, 517, 805
SfcI CTRYAG 1 cut(s) 666
Sfr274I CTCGAG 1 cut(s) 1104
SinI GGWCC 2 cut(s) 136, 833
SlaI CTCGAG 1 cut(s) 1104
SmiMI CAYNNNNRTG 2 cut(s) 590, 978
SmlI CTYRAG 4 cut(s) 163, 554, 838, 1104
SmoI CTYRAG 4 cut(s) 163, 554, 838, 1104
Sse9I AATT 7 cut(s) 104, 130, 187, 445, 790, 1039, 1148
SsiI CCGC 3 cut(s) 715, 831, 1024
SstI GAGCTC 1 cut(s) 164
StyD4I CCNGG 2 cut(s) 138, 636
StyI CCWWGG 2 cut(s) 409, 726
TaaI ACNGT 2 cut(s) 154, 223
TaiI ACGT 2 cut(s) 548, 819
TaqI TCGA 4 cut(s) 66, 301, 955, 1105
TaqII GACCGA 2 cut(s) 17, 261
TasI AATT 7 cut(s) 104, 130, 187, 445, 790, 1039, 1148
TatI WGTACW 1 cut(s) 698
TfiI GAWTC 2 cut(s) 68, 1141
Tru1I TTAA 1 cut(s) 858
Tru9I TTAA 1 cut(s) 858
TscAI CASTG 5 cut(s) 98, 228, 592, 876, 910
TseFI GTSAC 3 cut(s) 335, 440, 772
TseI GCWGC 3 cut(s) 425, 530, 993
Tsp45I GTSAC 3 cut(s) 335, 440, 772
TspDTI ATGAA 1 cut(s) 824
TspGWI ACGGA 2 cut(s) 134, 1049
TspRI CASTG 5 cut(s) 98, 228, 592, 876, 910
VpaK11BI GGWCC 2 cut(s) 136, 833
XapI RAATTY 3 cut(s) 130, 187, 1148
XceI RCATGY 1 cut(s) 1088
XhoI CTCGAG 1 cut(s) 1104
XmnI GAANNNNTTC 1 cut(s) 1120
Zsp2I ATGCAT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.