Rh2BG021600

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012553
Physical Location & Seq
Reverse (-)
1479340 .. 1484663
5324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG021600.1

Sequence Viewer

Length: 1143 bp
ATGTGTTTGAATGATGCAGAAATGGGAAACATGGAGAACAATAACATCAATACCAACAACAACAACAGCAAGCAGCAGTCTTTGCATTGTGAAAACGAGAAGGATAATTTGGACAGGGAATCTCCGGTGATGAGCTCCGATCAACAGAGCACCGTGTTGAATTCGTTTCAGCTCGAAAGCATATCTGAGGATTCAGTAGTTGTAGACCGAAAACAGAACATGTTACACTTTGTCCCTGACCTTCGGTCAGGAGAGTGGTCTGATATCGGAGGTCGTCCCCATATGGAAGATACTCATATATGCATCAGTGACTTGGCTAAGAAGTTTGGTTATAATGTACTCAATGATGAAGCTATCTCCTTCTATGGTGTATTTGATGGTCATGGGGGAAAGGATGCAGCACAATTTGTTCGTGACAACTTACCAAGAGTCATTGTTGAGGATTCTGACTTCCCTGTAGAGCTTGAAAAGGTGATAACAAGGTCATTTGTTGAGACAGATGCTGCATTTGCAAAAACTTGCTCACTTCGGTCTTCCCTTGCTTCTGGCACAACTGCACTCACTGCAATGATATTTGGGAGGTCTTTGCTCGTGGCGAATGCTGGAGATTGCAGGGCTATATTGTCGCGAAATGGAGCAGCTATAGAAATGTCAAAAGATCATAGACCCTGCTGCACAAAAGAAAGGACGCGAATTGAGTCCTTGGGTGGATATGTTGATGATGGTTATCTGAATGGTCAGTTAGGGGTCACCCGTGCATTAGGTGATTGGCATCTTGAAGGATTGAAGGATGAGGGTGAAAGGGGAGGACCCTTGAGTGCTGAACCGGAACTTAAATTGACAACACTGACCAAAGATGATGAATTTTTGATCATTGGTAGTGATGGAATATGGGATGTGTTCACCAACCAAAATGCTGTAGACTTTGCTCGAAGGAAACTCCAAGAGCACAATGATGTGAAGCTGTGTTGCAAGAAAATAGTAGAGGAGGCAATAAAACGAGGCGCAACCGACAATTTGACACTTGTTATGGTGAGTTTCCACTTGGAGCCACCTCCACATCTGGTGGTAGAAAGGTCTAGAGTCAGACGTAGTATTTCTGCTGAGGGACTACAAAATCTTCAGTTCTTCTTAGATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

380

Amino Acids

42.27

Weight (kDa)

4.98

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 92 - 339 2.2e-69 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 333
AccI GTMKAC 2 cut(s) 204, 921
AccII CGCG 2 cut(s) 628, 691
AcsI RAATTY 2 cut(s) 160, 863
AcuI CTGAAG 1 cut(s) 1106
AfaI GTAC 1 cut(s) 339
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 5 cut(s) 10, 160, 467, 779, 787
AhdI GACNNNNNGTC 1 cut(s) 244
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 6 cut(s) 135, 172, 353, 463, 641, 964
AluI AGCT 6 cut(s) 135, 172, 353, 463, 641, 964
Alw21I GWGCWC 3 cut(s) 137, 152, 951
Alw26I GTCTC 1 cut(s) 488
AlwNI CAGNNNCTG 1 cut(s) 503
ApeKI GCWGC 5 cut(s) 73, 398, 503, 638, 672
ApoI RAATTY 2 cut(s) 160, 863
AspLEI GCGC 1 cut(s) 1007
AspS9I GGNCC 1 cut(s) 809
AsuHPI GGTGA 7 cut(s) 139, 484, 742, 776, 809, 895, 1045
AvaII GGWCC 1 cut(s) 809
BanII GRGCYC 1 cut(s) 137
BauI CACGAG 1 cut(s) 590
BbsI GAAGAC 1 cut(s) 525
Bbv12I GWGCWC 3 cut(s) 137, 152, 951
BbvCI CCTCAGC 1 cut(s) 1104
BbvI GCAGC 5 cut(s) 85, 410, 490, 650, 659
BccI CCATC 3 cut(s) 371, 716, 878
BclI TGATCA 1 cut(s) 870
BcoDI GTCTC 1 cut(s) 488
BfaI CTAG 1 cut(s) 1080
BfmI CTRYAG 3 cut(s) 456, 642, 918
BisI GCNGC 5 cut(s) 74, 399, 504, 639, 673
BlsI GCNGC 5 cut(s) 75, 400, 505, 640, 674
Bme18I GGWCC 1 cut(s) 809
BmeRI GACNNNNNGTC 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 809
BmiI GGNNCC 2 cut(s) 811, 1050
BmsI GCATC 5 cut(s) 4, 312, 385, 490, 781
BpiI GAAGAC 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 624
Bpu10I CCTNAGC 1 cut(s) 1104
BpuEI CTTGAG 1 cut(s) 835
BsaBI GATNNNNATC 2 cut(s) 726, 771
BsaJI CCNNGG 1 cut(s) 702
BsaWI WCCGGW 2 cut(s) 124, 826
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 2 cut(s) 726, 771
BseDI CCNNGG 1 cut(s) 702
BseGI GGATG 3 cut(s) 400, 796, 901
BseJI GATNNNNATC 2 cut(s) 726, 771
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 2 cut(s) 177, 1095
BseRI GAGGAG 1 cut(s) 1001
BseXI GCAGC 5 cut(s) 85, 410, 490, 650, 659
BsgI GTGCAG 2 cut(s) 540, 658
Bsh1236I CGCG 2 cut(s) 628, 691
BsiHKAI GWGCWC 3 cut(s) 137, 152, 951
BsiSI CCGG 2 cut(s) 125, 827
BslFI GGGAC 3 cut(s) 218, 261, 1122
BsmAI GTCTC 1 cut(s) 488
BsmFI GGGAC 3 cut(s) 218, 261, 1122
BsmI GAATGC 1 cut(s) 604
Bsp1286I GDGCHC 3 cut(s) 137, 152, 951
Bsp143I GATC 4 cut(s) 139, 658, 870, 1135
Bsp68I TCGCGA 1 cut(s) 628
BspCNI CTCAG 2 cut(s) 178, 1096
BspFNI CGCG 2 cut(s) 628, 691
BspLI GGNNCC 2 cut(s) 811, 1050
BsrDI GCAATG 1 cut(s) 573
BssECI CCNNGG 1 cut(s) 702
BssMI GATC 4 cut(s) 139, 658, 870, 1135
BssSI CACGAG 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 702
Bst2BI CACGAG 1 cut(s) 590
Bst4CI ACNGT 1 cut(s) 154
BstAPI GCANNNNNTGC 2 cut(s) 82, 563
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 4 cut(s) 186, 318, 1104, 1132
BstEII GGTNACC 1 cut(s) 748
BstF5I GGATG 3 cut(s) 400, 796, 901
BstFNI CGCG 2 cut(s) 628, 691
BstHHI GCGC 1 cut(s) 1007
BstKTI GATC 4 cut(s) 142, 661, 873, 1138
BstMAI GTCTC 1 cut(s) 488
BstMBI GATC 4 cut(s) 139, 658, 870, 1135
BstMWI GCNNNNNNNGC 3 cut(s) 82, 509, 563
BstNSI RCATGY 1 cut(s) 223
BstPI GGTNACC 1 cut(s) 748
BstSFI CTRYAG 3 cut(s) 456, 642, 918
BstUI CGCG 2 cut(s) 628, 691
BstV1I GCAGC 5 cut(s) 85, 410, 490, 650, 659
BstV2I GAAGAC 1 cut(s) 525
BtsCI GGATG 3 cut(s) 400, 796, 901
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 3 cut(s) 313, 561, 845
BtuMI TCGCGA 1 cut(s) 628
Cac8I GCNNGC 1 cut(s) 71
CaiI CAGNNNCTG 1 cut(s) 503
CfoI GCGC 1 cut(s) 1007
Cfr13I GGNCC 1 cut(s) 809
CseI GACGC 1 cut(s) 697
Csp6I GTAC 1 cut(s) 338
CviAII CATG 3 cut(s) 31, 220, 383
CviJI RGCY 9 cut(s) 135, 172, 317, 353, 463, 617, 641, 964, 1051
CviKI_1 RGCY 9 cut(s) 135, 172, 317, 353, 463, 617, 641, 964, 1051
CviQI GTAC 1 cut(s) 338
DdeI CTNAG 4 cut(s) 186, 318, 1104, 1132
DpnI GATC 4 cut(s) 141, 660, 872, 1137
DpnII GATC 4 cut(s) 139, 658, 870, 1135
DriI GACNNNNNGTC 1 cut(s) 244
Eam1105I GACNNNNNGTC 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 702
Eco24I GRGCYC 1 cut(s) 137
Eco32I GATATC 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 809
Eco53kI GAGCTC 1 cut(s) 135
Eco57I CTGAAG 1 cut(s) 1106
Eco91I GGTNACC 1 cut(s) 748
EcoICRI GAGCTC 1 cut(s) 135
EcoO109I RGGNCCY 1 cut(s) 809
EcoO65I GGTNACC 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 160
EcoRV GATATC 1 cut(s) 265
EcoT14I CCWWGG 1 cut(s) 702
EcoT22I ATGCAT 1 cut(s) 305
EcoT38I GRGCYC 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 702
FaeI CATG 3 cut(s) 34, 223, 386
FaqI GGGAC 3 cut(s) 218, 261, 1122
FatI CATG 3 cut(s) 30, 219, 382
FauNDI CATATG 1 cut(s) 282
FbaI TGATCA 1 cut(s) 870
FblI GTMKAC 2 cut(s) 204, 921
Fnu4HI GCNGC 5 cut(s) 74, 399, 504, 639, 673
FokI GGATG 3 cut(s) 407, 803, 908
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 5 cut(s) 74, 399, 504, 639, 673
FspBI CTAG 1 cut(s) 1080
GlaI GCGC 1 cut(s) 1006
GluI GCNGC 5 cut(s) 74, 399, 504, 639, 673
GsuI CTGGAG 1 cut(s) 624
HapII CCGG 2 cut(s) 125, 827
HgaI GACGC 1 cut(s) 697
HhaI GCGC 1 cut(s) 1007
Hin1II CATG 3 cut(s) 34, 223, 386
Hin6I GCGC 1 cut(s) 1005
HinP1I GCGC 1 cut(s) 1005
HinfI GANTC 6 cut(s) 119, 191, 429, 443, 698, 1083
HpaII CCGG 2 cut(s) 125, 827
HphI GGTGA 7 cut(s) 139, 484, 742, 776, 809, 895, 1045
Hpy166II GTNNAC 3 cut(s) 205, 903, 922
Hpy188I TCNGA 7 cut(s) 139, 187, 262, 269, 448, 732, 1088
Hpy188III TCNNGA 5 cut(s) 249, 413, 627, 776, 1080
Hpy8I GTNNAC 3 cut(s) 205, 903, 922
HpyAV CCTTC 6 cut(s) 94, 251, 370, 773, 781, 927
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 1 cut(s) 1090
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 509, 563
HpyF3I CTNAG 4 cut(s) 186, 318, 1104, 1132
HpySE526I ACGT 1 cut(s) 1090
Hsp92II CATG 3 cut(s) 34, 223, 386
HspAI GCGC 1 cut(s) 1005
Ksp22I TGATCA 1 cut(s) 870
Kzo9I GATC 4 cut(s) 139, 658, 870, 1135
LmnI GCTCC 3 cut(s) 140, 635, 1048
Lsp1109I GCAGC 5 cut(s) 85, 410, 490, 650, 659
LweI GCATC 5 cut(s) 4, 312, 385, 490, 781
MaeI CTAG 1 cut(s) 1080
MaeII ACGT 1 cut(s) 1090
MaeIII GTNAC 4 cut(s) 222, 308, 413, 748
MalI GATC 4 cut(s) 141, 660, 872, 1137
MboI GATC 4 cut(s) 139, 658, 870, 1135
MboII GAAGA 4 cut(s) 299, 525, 1112, 1120
MhlI GDGCHC 3 cut(s) 137, 152, 951
MluCI AATT 7 cut(s) 106, 160, 404, 693, 836, 863, 1015
MlyI GAGTC 3 cut(s) 438, 707, 1092
Mph1103I ATGCAT 1 cut(s) 305
MseI TTAA 1 cut(s) 834
MslI CAYNNNNRTG 2 cut(s) 566, 954
MspI CCGG 2 cut(s) 125, 827
Mva1269I GAATGC 1 cut(s) 604
MvnI CGCG 2 cut(s) 628, 691
MwoI GCNNNNNNNGC 3 cut(s) 82, 509, 563
NdeI CATATG 1 cut(s) 282
NdeII GATC 4 cut(s) 139, 658, 870, 1135
NlaIII CATG 3 cut(s) 34, 223, 386
NlaIV GGNNCC 2 cut(s) 811, 1050
NmuCI GTSAC 3 cut(s) 308, 413, 748
NruI TCGCGA 1 cut(s) 628
NsiI ATGCAT 1 cut(s) 305
NspI RCATGY 1 cut(s) 223
PciI ACATGT 1 cut(s) 219
PctI GAATGC 1 cut(s) 604
PfeI GAWTC 3 cut(s) 119, 191, 443
PkrI GCNGC 5 cut(s) 75, 400, 505, 640, 674
PleI GAGTC 3 cut(s) 437, 706, 1091
PpsI GAGTC 3 cut(s) 437, 706, 1091
PpuMI RGGWCCY 1 cut(s) 809
PscI ACATGT 1 cut(s) 219
PsiI TTATAA 1 cut(s) 333
Psp124BI GAGCTC 1 cut(s) 137
Psp5II RGGWCCY 1 cut(s) 809
PspEI GGTNACC 1 cut(s) 748
PspN4I GGNNCC 2 cut(s) 811, 1050
PspPI GGNCC 1 cut(s) 809
PspPPI RGGWCCY 1 cut(s) 809
PstNI CAGNNNCTG 1 cut(s) 503
RruI TCGCGA 1 cut(s) 628
RsaI GTAC 1 cut(s) 339
RsaNI GTAC 1 cut(s) 338
RseI CAYNNNNRTG 2 cut(s) 566, 954
SacI GAGCTC 1 cut(s) 137
SaqAI TTAA 1 cut(s) 834
SatI GCNGC 5 cut(s) 74, 399, 504, 639, 673
Sau3AI GATC 4 cut(s) 139, 658, 870, 1135
Sau96I GGNCC 1 cut(s) 809
SchI GAGTC 3 cut(s) 438, 707, 1092
SduI GDGCHC 3 cut(s) 137, 152, 951
SfaNI GCATC 5 cut(s) 4, 312, 385, 490, 781
SfcI CTRYAG 3 cut(s) 456, 642, 918
SinI GGWCC 1 cut(s) 809
SmiMI CAYNNNNRTG 2 cut(s) 566, 954
SmlI CTYRAG 1 cut(s) 814
SmoI CTYRAG 1 cut(s) 814
Sse9I AATT 7 cut(s) 106, 160, 404, 693, 836, 863, 1015
SspMI CTAG 1 cut(s) 1080
SstI GAGCTC 1 cut(s) 137
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 1 cut(s) 1093
TaqI TCGA 2 cut(s) 174, 931
TaqII GACCGA 3 cut(s) 222, 234, 519
TasI AATT 7 cut(s) 106, 160, 404, 693, 836, 863, 1015
TatI WGTACW 1 cut(s) 337
TfiI GAWTC 3 cut(s) 119, 191, 443
Tru1I TTAA 1 cut(s) 834
Tru9I TTAA 1 cut(s) 834
TscAI CASTG 3 cut(s) 313, 568, 852
TseFI GTSAC 3 cut(s) 308, 413, 748
TseI GCWGC 5 cut(s) 73, 398, 503, 638, 672
Tsp45I GTSAC 3 cut(s) 308, 413, 748
TspDTI ATGAA 2 cut(s) 363, 876
TspRI CASTG 3 cut(s) 313, 568, 852
VpaK11BI GGWCC 1 cut(s) 809
XapI RAATTY 2 cut(s) 160, 863
XbaI TCTAGA 1 cut(s) 1079
XceI RCATGY 1 cut(s) 223
XmiI GTMKAC 2 cut(s) 204, 921
XspI CTAG 1 cut(s) 1080
Zsp2I ATGCAT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.