RLG00000015791

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012553
Physical Location & Seq
Reverse (-)
1459243 .. 1461535
2293 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015791

Sequence Viewer

Length: 1149 bp
ATGTGTTTGAATGATGCAGAAATGGGAAACATGGAGAACAATAACATCAATACCAACAACAACAACAACAACAGCAAGCAGCAGTCTTTGCATTGTGAAAACGAGAAGGATAATTTGGACAGGGAATCTCCGGTGATGAGCTCCAATCAACAGAGCACCGTGTTGAATTCGTTTCAGCTCGAAAGCATATCTGAGGATTCAGTAGTTGTAGACCGAAAACAGAACATGTTACACTTTGTCCCTGACCTTCGGTCAGGAGAGTGGTCTGATATAGGAGGTCGTCCCCATATGGAAGATACTCATATATGCATCAGTGACTTGGCTAAGAAGTTCGGTTACAATGTACTCAATGATGAAGCTATCTCCTTCTATGGTGTATTTGATGGTCATGGGGGAAAGGATGCAGCGCAATTTGTTCGTGACAACTTGCCAAGAGTCATCGTTGAGGATTCTGACTTCCCTGTAGAGCTTGAAAAGGTGATAACAAGGTCATTTGTTGAGACAGATGCTGCATTTGCAAAAACTTGCTCACTTCGGTCTTCCCTTGCTTCTGGCACAACTGCACTCACTGCAATGATATTTGGGAGGTCTTTGCTCGTGGCGAATGCTGGAGATTGCAGGGCTATATTGTCGCGAAATGGAGCAGCTATAGAAATGTCAAAAGATCATAGACCCTGCTGCACAAAAGAAAGGATGCGAATTGAGTCTTTGGGTGGATATGTTGATGATGGTTATCTGAATGGTCAGTTAGGGGTCACCCGTGCATTAGGTGATTGGCATCTTGAAGGATTGAAGGATGAGGGTGAAAGGGGAGGACCCTTGAGTGCTGAACCGGAACTTAAATTGACAACACTGACTAAAGATGATGAATTTTTGATCATTGGTAGTGATGGAATATGGGATGTGTTCACCAACCAAAATGCTGTAGACTTTGCTCGAAGGAAACTCCAAGTGCACAATGATGTGAAGCTGTGTTGCAAGCAAATAGTAGAGGAGGCAATAAAACGAGGCGCAACCGACAATTTGACACTTGTTATGGTGAGTTTCCACTTGGAGCCACCTCCACATCTGGTGGTAGAAAGGTCTAAAGTCAGACGTAGTATTTCTGCTGAGGGACTACAAAATCTTCAGTTTTTCTTAGATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

383

Amino Acids

42.47

Weight (kDa)

5.01

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 94 - 341 1.8e-68 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 210, 927
AccII CGCG 1 cut(s) 634
AcsI RAATTY 2 cut(s) 166, 869
AcuI CTGAAG 1 cut(s) 1112
AfaI GTAC 1 cut(s) 345
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 5 cut(s) 10, 166, 473, 785, 793
AhdI GACNNNNNGTC 1 cut(s) 250
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AluBI AGCT 6 cut(s) 141, 178, 359, 469, 647, 970
AluI AGCT 6 cut(s) 141, 178, 359, 469, 647, 970
Alw21I GWGCWC 3 cut(s) 143, 158, 957
Alw26I GTCTC 1 cut(s) 494
Alw44I GTGCAC 1 cut(s) 953
AlwNI CAGNNNCTG 1 cut(s) 509
ApaLI GTGCAC 1 cut(s) 953
ApeKI GCWGC 5 cut(s) 79, 404, 509, 644, 678
ApoI RAATTY 2 cut(s) 166, 869
AspLEI GCGC 2 cut(s) 409, 1013
AspS9I GGNCC 1 cut(s) 815
AsuHPI GGTGA 7 cut(s) 145, 490, 748, 782, 815, 901, 1051
AvaII GGWCC 1 cut(s) 815
BaeGI GKGCMC 1 cut(s) 957
BanII GRGCYC 1 cut(s) 143
BarI GAAGNNNNNNTAC 2 cut(s) 320, 352
BauI CACGAG 1 cut(s) 596
BbsI GAAGAC 1 cut(s) 531
Bbv12I GWGCWC 3 cut(s) 143, 158, 957
BbvCI CCTCAGC 1 cut(s) 1110
BbvI GCAGC 5 cut(s) 91, 416, 496, 656, 665
BccI CCATC 3 cut(s) 377, 722, 884
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BclI TGATCA 1 cut(s) 876
BcoDI GTCTC 1 cut(s) 494
BfmI CTRYAG 3 cut(s) 462, 648, 924
BisI GCNGC 5 cut(s) 80, 405, 510, 645, 679
BlsI GCNGC 5 cut(s) 81, 406, 511, 646, 680
Bme18I GGWCC 1 cut(s) 815
BmeRI GACNNNNNGTC 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 815
BmiI GGNNCC 2 cut(s) 817, 1056
BmsI GCATC 6 cut(s) 4, 318, 391, 496, 684, 787
BpiI GAAGAC 1 cut(s) 531
BpmI CTGGAG 1 cut(s) 630
Bpu10I CCTNAGC 1 cut(s) 1110
BpuEI CTTGAG 1 cut(s) 841
BsaBI GATNNNNATC 2 cut(s) 732, 777
BsaWI WCCGGW 2 cut(s) 130, 832
Bse3DI GCAATG 1 cut(s) 579
Bse8I GATNNNNATC 2 cut(s) 732, 777
BseGI GGATG 4 cut(s) 406, 699, 802, 907
BseJI GATNNNNATC 2 cut(s) 732, 777
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 2 cut(s) 183, 1101
BseRI GAGGAG 1 cut(s) 1007
BseSI GKGCMC 1 cut(s) 957
BseXI GCAGC 5 cut(s) 91, 416, 496, 656, 665
BsgI GTGCAG 2 cut(s) 546, 664
Bsh1236I CGCG 1 cut(s) 634
BsiHKAI GWGCWC 3 cut(s) 143, 158, 957
BsiSI CCGG 2 cut(s) 131, 833
BslFI GGGAC 3 cut(s) 224, 267, 1128
BsmAI GTCTC 1 cut(s) 494
BsmFI GGGAC 3 cut(s) 224, 267, 1128
BsmI GAATGC 1 cut(s) 610
Bsp1286I GDGCHC 3 cut(s) 143, 158, 957
Bsp143I GATC 3 cut(s) 664, 876, 1141
Bsp68I TCGCGA 1 cut(s) 634
BspCNI CTCAG 2 cut(s) 184, 1102
BspFNI CGCG 1 cut(s) 634
BspLI GGNNCC 2 cut(s) 817, 1056
BsrDI GCAATG 1 cut(s) 579
BssMI GATC 3 cut(s) 664, 876, 1141
BssSI CACGAG 1 cut(s) 596
Bst2BI CACGAG 1 cut(s) 596
Bst4CI ACNGT 1 cut(s) 160
BstAPI GCANNNNNTGC 2 cut(s) 88, 569
BstC8I GCNNGC 2 cut(s) 77, 980
BstDEI CTNAG 4 cut(s) 192, 324, 1110, 1138
BstEII GGTNACC 1 cut(s) 754
BstF5I GGATG 4 cut(s) 406, 699, 802, 907
BstFNI CGCG 1 cut(s) 634
BstHHI GCGC 2 cut(s) 409, 1013
BstKTI GATC 3 cut(s) 667, 879, 1144
BstMAI GTCTC 1 cut(s) 494
BstMBI GATC 3 cut(s) 664, 876, 1141
BstMWI GCNNNNNNNGC 3 cut(s) 88, 515, 569
BstNSI RCATGY 1 cut(s) 229
BstPI GGTNACC 1 cut(s) 754
BstSFI CTRYAG 3 cut(s) 462, 648, 924
BstSLI GKGCMC 1 cut(s) 957
BstUI CGCG 1 cut(s) 634
BstV1I GCAGC 5 cut(s) 91, 416, 496, 656, 665
BstV2I GAAGAC 1 cut(s) 531
BtsCI GGATG 4 cut(s) 406, 699, 802, 907
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 3 cut(s) 319, 567, 851
BtuMI TCGCGA 1 cut(s) 634
Cac8I GCNNGC 2 cut(s) 77, 980
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 2 cut(s) 409, 1013
Cfr13I GGNCC 1 cut(s) 815
Csp6I GTAC 1 cut(s) 344
CviAII CATG 3 cut(s) 31, 226, 389
CviJI RGCY 9 cut(s) 141, 178, 323, 359, 469, 623, 647, 970, 1057
CviKI_1 RGCY 9 cut(s) 141, 178, 323, 359, 469, 623, 647, 970, 1057
CviQI GTAC 1 cut(s) 344
DdeI CTNAG 4 cut(s) 192, 324, 1110, 1138
DpnI GATC 3 cut(s) 666, 878, 1143
DpnII GATC 3 cut(s) 664, 876, 1141
DriI GACNNNNNGTC 1 cut(s) 250
Eam1105I GACNNNNNGTC 1 cut(s) 250
Ecl136II GAGCTC 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 815
Eco53kI GAGCTC 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 1112
Eco91I GGTNACC 1 cut(s) 754
EcoICRI GAGCTC 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 815
EcoO65I GGTNACC 1 cut(s) 754
EcoRI GAATTC 1 cut(s) 166
EcoT22I ATGCAT 1 cut(s) 311
EcoT38I GRGCYC 1 cut(s) 143
FaeI CATG 3 cut(s) 34, 229, 392
FaqI GGGAC 3 cut(s) 224, 267, 1128
FatI CATG 3 cut(s) 30, 225, 388
FauNDI CATATG 1 cut(s) 288
FbaI TGATCA 1 cut(s) 876
FblI GTMKAC 2 cut(s) 210, 927
Fnu4HI GCNGC 5 cut(s) 80, 405, 510, 645, 679
FokI GGATG 4 cut(s) 413, 706, 809, 914
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 5 cut(s) 80, 405, 510, 645, 679
GlaI GCGC 2 cut(s) 408, 1012
GluI GCNGC 5 cut(s) 80, 405, 510, 645, 679
GsuI CTGGAG 1 cut(s) 630
HapII CCGG 2 cut(s) 131, 833
HhaI GCGC 2 cut(s) 409, 1013
Hin1II CATG 3 cut(s) 34, 229, 392
Hin6I GCGC 2 cut(s) 407, 1011
HinP1I GCGC 2 cut(s) 407, 1011
HinfI GANTC 5 cut(s) 125, 197, 435, 449, 704
HpaII CCGG 2 cut(s) 131, 833
HphI GGTGA 7 cut(s) 145, 490, 748, 782, 815, 901, 1051
Hpy166II GTNNAC 4 cut(s) 211, 909, 928, 955
Hpy188I TCNGA 5 cut(s) 193, 268, 454, 738, 1094
Hpy188III TCNNGA 4 cut(s) 255, 419, 633, 782
Hpy8I GTNNAC 4 cut(s) 211, 909, 928, 955
HpyAV CCTTC 6 cut(s) 100, 257, 376, 779, 787, 933
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4IV ACGT 1 cut(s) 1096
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 515, 569
HpyF3I CTNAG 4 cut(s) 192, 324, 1110, 1138
HpySE526I ACGT 1 cut(s) 1096
Hsp92II CATG 3 cut(s) 34, 229, 392
HspAI GCGC 2 cut(s) 407, 1011
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 3 cut(s) 664, 876, 1141
LmnI GCTCC 3 cut(s) 146, 641, 1054
Lsp1109I GCAGC 5 cut(s) 91, 416, 496, 656, 665
LweI GCATC 6 cut(s) 4, 318, 391, 496, 684, 787
MaeII ACGT 1 cut(s) 1096
MaeIII GTNAC 5 cut(s) 228, 314, 335, 419, 754
MalI GATC 3 cut(s) 666, 878, 1143
MboI GATC 3 cut(s) 664, 876, 1141
MboII GAAGA 3 cut(s) 305, 531, 1118
MhlI GDGCHC 3 cut(s) 143, 158, 957
MluCI AATT 7 cut(s) 112, 166, 410, 699, 842, 869, 1021
MlyI GAGTC 2 cut(s) 444, 713
Mph1103I ATGCAT 1 cut(s) 311
MseI TTAA 1 cut(s) 840
MslI CAYNNNNRTG 2 cut(s) 572, 960
MspI CCGG 2 cut(s) 131, 833
Mva1269I GAATGC 1 cut(s) 610
MvnI CGCG 1 cut(s) 634
MwoI GCNNNNNNNGC 3 cut(s) 88, 515, 569
NdeI CATATG 1 cut(s) 288
NdeII GATC 3 cut(s) 664, 876, 1141
NlaIII CATG 3 cut(s) 34, 229, 392
NlaIV GGNNCC 2 cut(s) 817, 1056
NmuCI GTSAC 3 cut(s) 314, 419, 754
NruI TCGCGA 1 cut(s) 634
NsiI ATGCAT 1 cut(s) 311
NspI RCATGY 1 cut(s) 229
PciI ACATGT 1 cut(s) 225
PctI GAATGC 1 cut(s) 610
PfeI GAWTC 3 cut(s) 125, 197, 449
PkrI GCNGC 5 cut(s) 81, 406, 511, 646, 680
PleI GAGTC 2 cut(s) 443, 712
PpsI GAGTC 2 cut(s) 443, 712
PpuMI RGGWCCY 1 cut(s) 815
PscI ACATGT 1 cut(s) 225
Psp124BI GAGCTC 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 815
PspEI GGTNACC 1 cut(s) 754
PspN4I GGNNCC 2 cut(s) 817, 1056
PspPI GGNCC 1 cut(s) 815
PspPPI RGGWCCY 1 cut(s) 815
PstNI CAGNNNCTG 1 cut(s) 509
RruI TCGCGA 1 cut(s) 634
RsaI GTAC 1 cut(s) 345
RsaNI GTAC 1 cut(s) 344
RseI CAYNNNNRTG 2 cut(s) 572, 960
SacI GAGCTC 1 cut(s) 143
SaqAI TTAA 1 cut(s) 840
SatI GCNGC 5 cut(s) 80, 405, 510, 645, 679
Sau3AI GATC 3 cut(s) 664, 876, 1141
Sau96I GGNCC 1 cut(s) 815
SchI GAGTC 2 cut(s) 444, 713
SduI GDGCHC 3 cut(s) 143, 158, 957
SfaNI GCATC 6 cut(s) 4, 318, 391, 496, 684, 787
SfcI CTRYAG 3 cut(s) 462, 648, 924
SinI GGWCC 1 cut(s) 815
SmiMI CAYNNNNRTG 2 cut(s) 572, 960
SmlI CTYRAG 1 cut(s) 820
SmoI CTYRAG 1 cut(s) 820
Sse9I AATT 7 cut(s) 112, 166, 410, 699, 842, 869, 1021
SstI GAGCTC 1 cut(s) 143
TaaI ACNGT 1 cut(s) 160
TaiI ACGT 1 cut(s) 1099
TaqI TCGA 2 cut(s) 180, 937
TaqII GACCGA 3 cut(s) 228, 240, 525
TasI AATT 7 cut(s) 112, 166, 410, 699, 842, 869, 1021
TatI WGTACW 1 cut(s) 343
TfiI GAWTC 3 cut(s) 125, 197, 449
Tru1I TTAA 1 cut(s) 840
Tru9I TTAA 1 cut(s) 840
TscAI CASTG 3 cut(s) 319, 574, 858
TseFI GTSAC 3 cut(s) 314, 419, 754
TseI GCWGC 5 cut(s) 79, 404, 509, 644, 678
Tsp45I GTSAC 3 cut(s) 314, 419, 754
TspDTI ATGAA 2 cut(s) 369, 882
TspRI CASTG 3 cut(s) 319, 574, 858
VneI GTGCAC 1 cut(s) 953
VpaK11BI GGWCC 1 cut(s) 815
XapI RAATTY 2 cut(s) 166, 869
XceI RCATGY 1 cut(s) 229
XmiI GTMKAC 2 cut(s) 210, 927
Zsp2I ATGCAT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.