MD15G1160600.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012553
Physical Location & Seq
Reverse (-)
12017518 .. 12020146
2629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1160600.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGTGCCTGATAGACGCAGAACAGCTCGGTCAAGAAATGGGCAACAGGGAGAATAACAAGAACATCGATTCCAACGACAATGACAACGAGCAGTGGTCTCTGCAAGTCGACCGGAAGGAGGAGAATTCGGACCGGGAAACTCCTGCTGTGGTGAGCTCAAGTACCCAGAAAAACATGCTCAATTCGTTTCAACTTGAAAGCATAGGTGAGGATACAGTGGTTGCAGACAGAAAGCAAAGCCTGTTCAACTTTGTTCCTGCCCTCAGGTCAGGAGAGTGGTCTGATATTGGGGGTCGAACCCATATGGAGGATACTCATTTATGCATTGGTGACTTAGCTAAGAAGTTTGGTTATAATCTACTCAGTGAGGAAGCTATCTCTTTCTATGGCGTATTTGATGGTCACGGAGGAAAGGATGCAGCACACTTTGTTCGTGACAATTTACCAAGAGTCATTGTTGAGGATGCTGACTTTCCTTTAGAACTTGAAAAGGTGATAACAAGGTCATTTATGGAGACAGATGCTGCGTTTGCAAACAAGTGTTCTCGTGAGGCTTCCCTTGCTTCCGGCACAACAGCACTCACTGCAATGATATTTGGGAGGTCTTTGCTTGTGGCAAATGCCGGAGATTGCCGGGCTGTATTATCACGATGTGGAGTGGCTATAGAAATGTCGAAGGATCATAGGCCCTGTTGCACAAAAGAAAGAATGCGTATTGAGTCCTTGGGTGGATATATTGATGATGGGTATCTAAATGGTCAGTTAGGCGTAACCCGTGCATTAGGTAATTGGCATCTTGAAGGAATGAAGGATGCGGGTGAAAGGGGCGGACCCTTGAGCGCTAAACCAGAACTTAAACTGATGACACTGAGCAAAGACGATGAGTTTTTGATCATTGGCACTGATGGGATATGGGAGATGTTCACCAGCCAAAACGCTGTTGATTTCGCTCGAAGGAGACTGCAAGAGCACAATGATGTGAAGTTGTGCTGCAAGCAAATAGTAGAGGAGGCAATAAAGCGGGGAGCAACAGACAATTTGACGCTTGTTATGGTGAGTTTCCACTTGGAGCCACCCCCACATGTGCTGGTAGAGAGACCTAGATTCAGACGAAGCATTTCTGCCGAGGGACTTCAGAATCTCAGATTCTTCTTAGATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

388

Amino Acids

43.14

Weight (kDa)

5.11

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 98 - 346 1.9e-65 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 354
AccI GTMKAC 1 cut(s) 108
AciI CCGC 3 cut(s) 815, 828, 1021
AclWI GGATC 1 cut(s) 687
AcsI RAATTY 1 cut(s) 124
AcuI CTGAAG 1 cut(s) 1118
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 1 cut(s) 163
AfeI AGCGCT 1 cut(s) 841
AfiI CCNNNNNNNGG 2 cut(s) 118, 307
AflIII ACRYGT 1 cut(s) 1081
AgsI TTSAA 5 cut(s) 191, 197, 247, 488, 800
AloI GAACNNNNNNTCC 2 cut(s) 53, 85
AluBI AGCT 4 cut(s) 25, 156, 338, 374
AluI AGCT 4 cut(s) 25, 156, 338, 374
Alw21I GWGCWC 2 cut(s) 158, 972
Alw26I GTCTC 4 cut(s) 102, 509, 952, 1090
AlwI GGATC 1 cut(s) 687
AlwNI CAGNNNCTG 1 cut(s) 524
Aor51HI AGCGCT 1 cut(s) 841
AoxI GGCC 1 cut(s) 686
ApeKI GCWGC 3 cut(s) 419, 524, 990
ApoI RAATTY 1 cut(s) 124
Asp700I GAANNNNTTC 1 cut(s) 1117
AspLEI GCGC 1 cut(s) 842
AspS9I GGNCC 3 cut(s) 130, 687, 830
AsuC2I CCSGG 2 cut(s) 134, 635
AsuHPI GGTGA 7 cut(s) 163, 218, 341, 505, 830, 916, 1066
AvaII GGWCC 2 cut(s) 130, 830
AxyI CCTNAGG 1 cut(s) 263
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BanII GRGCYC 1 cut(s) 158
BauI CACGAG 1 cut(s) 546
Bbv12I GWGCWC 2 cut(s) 158, 972
BbvI GCAGC 3 cut(s) 431, 511, 977
BccI CCATC 4 cut(s) 392, 737, 899, 1151
BciVI GTATCC 2 cut(s) 205, 304
BclI TGATCA 1 cut(s) 891
BcnI CCSGG 2 cut(s) 134, 635
BcoDI GTCTC 4 cut(s) 102, 509, 952, 1090
BfaI CTAG 1 cut(s) 1101
BfmI CTRYAG 1 cut(s) 663
BfoI RGCGCY 1 cut(s) 843
BfuI GTATCC 2 cut(s) 205, 304
BisI GCNGC 3 cut(s) 420, 525, 991
BlsI GCNGC 3 cut(s) 421, 526, 992
Bme1390I CCNGG 2 cut(s) 134, 635
Bme18I GGWCC 2 cut(s) 130, 830
BmgT120I GGNCC 3 cut(s) 130, 687, 830
BmiI GGNNCC 2 cut(s) 832, 1071
BmrFI CCNGG 2 cut(s) 134, 635
BmsI GCATC 5 cut(s) 406, 454, 511, 802, 802
BpuEI CTTGAG 2 cut(s) 142, 856
BpuMI CCSGG 2 cut(s) 134, 635
Bsa29I ATCGAT 1 cut(s) 66
BsaBI GATNNNNATC 1 cut(s) 747
BsaI GGTCTC 2 cut(s) 102, 1090
BsaJI CCNNGG 2 cut(s) 723, 1125
BsaWI WCCGGW 1 cut(s) 111
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 307
Bse21I CCTNAGG 1 cut(s) 263
Bse3DI GCAATG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 747
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 723, 1125
BseGI GGATG 3 cut(s) 421, 469, 817
BseJI GATNNNNATC 1 cut(s) 747
BseLI CCNNNNNNNGG 2 cut(s) 118, 307
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 4 cut(s) 277, 376, 860, 1156
BseRI GAGGAG 2 cut(s) 134, 1022
BseXI GCAGC 3 cut(s) 431, 511, 977
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 1 cut(s) 688
BshVI ATCGAT 1 cut(s) 66
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 2 cut(s) 158, 972
BsiSI CCGG 5 cut(s) 112, 133, 567, 624, 634
BslFI GGGAC 1 cut(s) 1143
BslI CCNNNNNNNGG 2 cut(s) 118, 307
BsmAI GTCTC 4 cut(s) 102, 509, 952, 1090
BsmFI GGGAC 1 cut(s) 1143
BsmI GAATGC 1 cut(s) 714
BsnI GGCC 1 cut(s) 688
Bso31I GGTCTC 2 cut(s) 102, 1090
Bsp1286I GDGCHC 2 cut(s) 158, 972
Bsp143I GATC 2 cut(s) 679, 891
BspACI CCGC 3 cut(s) 815, 828, 1021
BspANI GGCC 1 cut(s) 688
BspCNI CTCAG 4 cut(s) 276, 375, 861, 1155
BspDI ATCGAT 1 cut(s) 66
BspLI GGNNCC 2 cut(s) 832, 1071
BspPI GGATC 1 cut(s) 687
BspTNI GGTCTC 2 cut(s) 102, 1090
BsrDI GCAATG 1 cut(s) 594
BssECI CCNNGG 2 cut(s) 723, 1125
BssMI GATC 2 cut(s) 679, 891
BssSI CACGAG 1 cut(s) 546
BssT1I CCWWGG 1 cut(s) 723
Bst2BI CACGAG 1 cut(s) 546
Bst4CI ACNGT 1 cut(s) 217
BstAPI GCANNNNNTGC 1 cut(s) 584
BstC8I GCNNGC 1 cut(s) 995
BstDEI CTNAG 7 cut(s) 263, 334, 339, 362, 869, 1142, 1153
BstF5I GGATG 3 cut(s) 421, 469, 817
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 1 cut(s) 842
BstKTI GATC 2 cut(s) 682, 894
BstMAI GTCTC 4 cut(s) 102, 509, 952, 1090
BstMBI GATC 2 cut(s) 679, 891
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 3 cut(s) 530, 560, 584
BstNSI RCATGY 2 cut(s) 178, 1085
BstSCI CCNGG 2 cut(s) 132, 633
BstSFI CTRYAG 1 cut(s) 663
BstV1I GCAGC 3 cut(s) 431, 511, 977
Bsu15I ATCGAT 1 cut(s) 66
Bsu36I CCTNAGG 1 cut(s) 263
BsuI GTATCC 2 cut(s) 205, 304
BsuRI GGCC 1 cut(s) 688
BsuTUI ATCGAT 1 cut(s) 66
BtsCI GGATG 3 cut(s) 421, 469, 817
BtsI GCAGTG 2 cut(s) 98, 582
BtsIMutI CAGTG 6 cut(s) 98, 222, 370, 582, 866, 900
Cac8I GCNNGC 1 cut(s) 995
CaiI CAGNNNCTG 1 cut(s) 524
CfoI GCGC 1 cut(s) 842
Cfr13I GGNCC 3 cut(s) 130, 687, 830
ClaI ATCGAT 1 cut(s) 66
CpoI CGGWCCG 1 cut(s) 130
CseI GACGC 2 cut(s) 23, 1051
Csp6I GTAC 1 cut(s) 162
CspI CGGWCCG 1 cut(s) 130
CviAII CATG 2 cut(s) 175, 1082
CviQI GTAC 1 cut(s) 162
DdeI CTNAG 7 cut(s) 263, 334, 339, 362, 869, 1142, 1153
DpnI GATC 2 cut(s) 681, 893
DpnII GATC 2 cut(s) 679, 891
DraIII CACNNNGTG 1 cut(s) 653
EciI GGCGGA 1 cut(s) 843
Ecl136II GAGCTC 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 723
Eco24I GRGCYC 1 cut(s) 158
Eco31I GGTCTC 2 cut(s) 102, 1090
Eco47I GGWCC 2 cut(s) 130, 830
Eco47III AGCGCT 1 cut(s) 841
Eco53kI GAGCTC 1 cut(s) 156
Eco57I CTGAAG 1 cut(s) 1118
Eco81I CCTNAGG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 156
EcoO109I RGGNCCY 1 cut(s) 687
EcoRI GAATTC 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 723
EcoT22I ATGCAT 1 cut(s) 326
EcoT38I GRGCYC 1 cut(s) 158
ErhI CCWWGG 1 cut(s) 723
FaeI CATG 2 cut(s) 178, 1085
FaqI GGGAC 1 cut(s) 1143
FatI CATG 2 cut(s) 174, 1081
FauI CCCGC 2 cut(s) 808, 1014
FauNDI CATATG 1 cut(s) 303
FbaI TGATCA 1 cut(s) 891
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 3 cut(s) 420, 525, 991
FokI GGATG 3 cut(s) 428, 476, 824
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 3 cut(s) 420, 525, 991
FspBI CTAG 1 cut(s) 1101
GlaI GCGC 1 cut(s) 841
GluI GCNGC 3 cut(s) 420, 525, 991
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 1 cut(s) 688
HapII CCGG 5 cut(s) 112, 133, 567, 624, 634
HgaI GACGC 2 cut(s) 23, 1051
HhaI GCGC 1 cut(s) 842
Hin1II CATG 2 cut(s) 178, 1085
Hin6I GCGC 1 cut(s) 840
HinP1I GCGC 1 cut(s) 840
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 6 cut(s) 68, 450, 719, 1104, 1138, 1146
HpaII CCGG 5 cut(s) 112, 133, 567, 624, 634
HphI GGTGA 7 cut(s) 163, 218, 341, 505, 830, 916, 1066
Hpy166II GTNNAC 2 cut(s) 109, 924
Hpy188I TCNGA 5 cut(s) 130, 283, 1109, 1137, 1145
Hpy188III TCNNGA 6 cut(s) 32, 270, 434, 548, 648, 797
Hpy8I GTNNAC 2 cut(s) 109, 924
HpyAV CCTTC 5 cut(s) 109, 670, 794, 802, 948
HpyCH4III ACNGT 1 cut(s) 217
HpyF10VI GCNNNNNNNGC 3 cut(s) 530, 560, 584
HpyF3I CTNAG 7 cut(s) 263, 334, 339, 362, 869, 1142, 1153
Hsp92II CATG 2 cut(s) 178, 1085
HspAI GCGC 1 cut(s) 840
Ksp22I TGATCA 1 cut(s) 891
Kzo9I GATC 2 cut(s) 679, 891
LmnI GCTCC 2 cut(s) 1025, 1069
Lsp1109I GCAGC 3 cut(s) 431, 511, 977
LweI GCATC 5 cut(s) 406, 454, 511, 802, 802
MaeI CTAG 1 cut(s) 1101
MaeIII GTNAC 4 cut(s) 329, 401, 434, 769
MalI GATC 2 cut(s) 681, 893
MboI GATC 2 cut(s) 679, 891
MboII GAAGA 1 cut(s) 1141
MhlI GDGCHC 2 cut(s) 158, 972
MluCI AATT 5 cut(s) 124, 181, 439, 787, 1036
MlyI GAGTC 2 cut(s) 459, 728
MmeI TCCRAC 1 cut(s) 96
Mph1103I ATGCAT 1 cut(s) 326
MroXI GAANNNNTTC 1 cut(s) 1117
MseI TTAA 1 cut(s) 855
MslI CAYNNNNRTG 2 cut(s) 587, 975
MspI CCGG 5 cut(s) 112, 133, 567, 624, 634
MspR9I CCNGG 2 cut(s) 134, 635
Mva1269I GAATGC 1 cut(s) 714
MwoI GCNNNNNNNGC 3 cut(s) 530, 560, 584
NciI CCSGG 2 cut(s) 134, 635
NdeI CATATG 1 cut(s) 303
NdeII GATC 2 cut(s) 679, 891
NlaIII CATG 2 cut(s) 178, 1085
NlaIV GGNNCC 2 cut(s) 832, 1071
NmeAIII GCCGAG 1 cut(s) 1150
NmuCI GTSAC 3 cut(s) 329, 401, 434
NsiI ATGCAT 1 cut(s) 326
NspI RCATGY 2 cut(s) 178, 1085
PciI ACATGT 1 cut(s) 1081
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 1 cut(s) 714
PdmI GAANNNNTTC 1 cut(s) 1117
PfeI GAWTC 4 cut(s) 68, 1104, 1138, 1146
PkrI GCNGC 3 cut(s) 421, 526, 992
PleI GAGTC 2 cut(s) 458, 727
PpsI GAGTC 2 cut(s) 458, 727
PscI ACATGT 1 cut(s) 1081
PsiI TTATAA 1 cut(s) 354
Psp124BI GAGCTC 1 cut(s) 158
PspN4I GGNNCC 2 cut(s) 832, 1071
PspPI GGNCC 3 cut(s) 130, 687, 830
PstNI CAGNNNCTG 1 cut(s) 524
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
RseI CAYNNNNRTG 2 cut(s) 587, 975
Rsr2I CGGWCCG 1 cut(s) 130
RsrII CGGWCCG 1 cut(s) 130
SacI GAGCTC 1 cut(s) 158
SalI GTCGAC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 855
SatI GCNGC 3 cut(s) 420, 525, 991
Sau3AI GATC 2 cut(s) 679, 891
Sau96I GGNCC 3 cut(s) 130, 687, 830
SchI GAGTC 2 cut(s) 459, 728
ScrFI CCNGG 2 cut(s) 134, 635
SduI GDGCHC 2 cut(s) 158, 972
SfaNI GCATC 5 cut(s) 406, 454, 511, 802, 802
SfcI CTRYAG 1 cut(s) 663
SinI GGWCC 2 cut(s) 130, 830
SmiMI CAYNNNNRTG 2 cut(s) 587, 975
SmlI CTYRAG 2 cut(s) 157, 835
SmoI CTYRAG 2 cut(s) 157, 835
Sse9I AATT 5 cut(s) 124, 181, 439, 787, 1036
SsiI CCGC 3 cut(s) 815, 828, 1021
SspMI CTAG 1 cut(s) 1101
SstI GAGCTC 1 cut(s) 158
StyD4I CCNGG 2 cut(s) 132, 633
StyI CCWWGG 1 cut(s) 723
TaaI ACNGT 1 cut(s) 217
TaqI TCGA 5 cut(s) 66, 108, 295, 674, 952
TaqII GACCGA 1 cut(s) 17
TasI AATT 5 cut(s) 124, 181, 439, 787, 1036
TfiI GAWTC 4 cut(s) 68, 1104, 1138, 1146
Tru1I TTAA 1 cut(s) 855
Tru9I TTAA 1 cut(s) 855
TscAI CASTG 6 cut(s) 98, 222, 370, 589, 873, 907
TseFI GTSAC 3 cut(s) 329, 401, 434
TseI GCWGC 3 cut(s) 419, 524, 990
Tsp45I GTSAC 3 cut(s) 329, 401, 434
TspDTI ATGAA 1 cut(s) 821
TspGWI ACGGA 1 cut(s) 420
TspRI CASTG 6 cut(s) 98, 222, 370, 589, 873, 907
VpaK11BI GGWCC 2 cut(s) 130, 830
XapI RAATTY 1 cut(s) 124
XceI RCATGY 2 cut(s) 178, 1085
XmiI GTMKAC 1 cut(s) 108
XmnI GAANNNNTTC 1 cut(s) 1117
XspI CTAG 1 cut(s) 1101
Zsp2I ATGCAT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.