Rw2G001750

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012553
Physical Location & Seq
Reverse (-)
1432163 .. 1435089
2927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001750.1

Sequence Viewer

Length: 1146 bp
ATGTGTTTGAATGATGCGGAAATGGGAAACATGGAGAACAGTAACATCAATACCAACAACAACAACAACAGCAAGCAGCAGTCTTTGCATTGTGAAAACGAGAAGGGTAATTTGGAGAAGGAATCTTCGGTGATGAGCTCCGATCAACAGAGCACCGTGTTGAATTCTTTTCAGCTTGAAAGCATATCTGAGGATTCAGTAGCTGTGGACCAAAAACAGAACATGTCACATTTTGTCCCTGCCCTTCGATCAGGAGAGTGGTCTGATATAGGAGATCGTCCCCATATGGAAGATACTCATATATGCATCAGTGACTTGGCTAAGAAGTTTGGTTATAATGTACTCAATGATGAAGCTATCTCCTTCTATGGTGTATTTGATGGTCATGGGGGAAAGGATGCAGCGCAATTTGTTCGTGACAACCTGCCAAGAGTCATCGTTGAGGATGCTGACTTCCCTGTAGAGCTTGAAAAGGTGATAACAAGGTCATTTGTTGAGACAGATGCTGCATTTGCAAAAACTTGCTCACTTCAGTCTTCCCTTGCTTCTGGCACAACTGCACTCACTGCAATGATATTTGGGAGGTCTTTGCTTGTGGCGAATGCTGGAGATTGCAGGGCTATATTGTCGCGAAATGGAGCAGCTATAGAAATGTCAAAAGATCATAGACCCTGCTGCACAAAAGAAAGGATGCGAATTGAGTCTTTGGGTGGATATGTTGATGATGGTTATCTGAATGGTCAGTTAGGGGTCACCCGTGCATTAGGTGATTGGCATCTTGAAGGATTGAAGGATGAGGGTGAAAGGGGAGGACCCTTGAGTGCTGAACCGGAACTTAAATTGACAACACTGACCAAAGATGATGAATTTTTGATCATTGGTAGTGATGGAATATGGGATGTGTTCACCAACCAAAATGCTGTAGATTTTGCTCGAAGGAAACTCCAAGAGCGCAATGATGTGAAGCTGTGTTGCAAGCAAATAGTAGAGGAGGCAATAAAACGAGGCGCAACCGACAATTTGACACTTGTTATGGTGAGTTTCCACTTGGAGCCACCTCCACATCTGGTGGTAGAAAGGTCTAGAGTCAGACGTAGTATTTCTGCTGAGGGACTACAAAATCTTCAGTCCTTCTTAGATCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

381

Amino Acids

42.18

Weight (kDa)

4.94

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 94 - 340 3.6e-68 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
Acc36I ACCTGC 1 cut(s) 432
AccII CGCG 1 cut(s) 631
AciI CCGC 1 cut(s) 17
AcsI RAATTY 2 cut(s) 163, 866
AcuI CTGAAG 2 cut(s) 515, 1109
AfaI GTAC 1 cut(s) 342
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 6 cut(s) 10, 163, 179, 470, 782, 790
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 7 cut(s) 138, 175, 203, 356, 466, 644, 967
AluI AGCT 7 cut(s) 138, 175, 203, 356, 466, 644, 967
Alw21I GWGCWC 2 cut(s) 140, 155
Alw26I GTCTC 1 cut(s) 491
AlwNI CAGNNNCTG 2 cut(s) 203, 506
ApeKI GCWGC 5 cut(s) 76, 401, 506, 641, 675
ApoI RAATTY 2 cut(s) 163, 866
AspLEI GCGC 3 cut(s) 406, 954, 1010
AspS9I GGNCC 2 cut(s) 208, 812
AsuHPI GGTGA 7 cut(s) 142, 487, 745, 779, 812, 898, 1048
AvaII GGWCC 2 cut(s) 208, 812
BanII GRGCYC 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 2 cut(s) 140, 155
BbvCI CCTCAGC 1 cut(s) 1107
BbvI GCAGC 5 cut(s) 88, 413, 493, 653, 662
BccI CCATC 3 cut(s) 374, 719, 881
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BclI TGATCA 1 cut(s) 873
BcoDI GTCTC 1 cut(s) 491
BfaI CTAG 1 cut(s) 1083
BfmI CTRYAG 3 cut(s) 459, 645, 921
BfuAI ACCTGC 1 cut(s) 432
BisI GCNGC 5 cut(s) 77, 402, 507, 642, 676
BlsI GCNGC 5 cut(s) 78, 403, 508, 643, 677
Bme18I GGWCC 2 cut(s) 208, 812
BmgT120I GGNCC 2 cut(s) 208, 812
BmiI GGNNCC 2 cut(s) 814, 1053
BmsI GCATC 7 cut(s) 4, 315, 388, 436, 493, 681, 784
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 627
Bpu10I CCTNAGC 1 cut(s) 1107
BpuEI CTTGAG 1 cut(s) 838
Bsa29I ATCGAT 1 cut(s) 1141
BsaBI GATNNNNATC 2 cut(s) 729, 774
BsaWI WCCGGW 1 cut(s) 829
Bse3DI GCAATG 2 cut(s) 576, 961
Bse8I GATNNNNATC 2 cut(s) 729, 774
BseCI ATCGAT 1 cut(s) 1141
BseGI GGATG 5 cut(s) 403, 451, 696, 799, 904
BseJI GATNNNNATC 2 cut(s) 729, 774
BseMI GCAATG 2 cut(s) 576, 961
BseMII CTCAG 2 cut(s) 180, 1098
BseRI GAGGAG 1 cut(s) 1004
BseXI GCAGC 5 cut(s) 88, 413, 493, 653, 662
BsgI GTGCAG 2 cut(s) 543, 661
Bsh1236I CGCG 1 cut(s) 631
BshVI ATCGAT 1 cut(s) 1141
BsiHKAI GWGCWC 2 cut(s) 140, 155
BsiSI CCGG 1 cut(s) 830
BslFI GGGAC 3 cut(s) 221, 264, 1125
BsmAI GTCTC 1 cut(s) 491
BsmFI GGGAC 3 cut(s) 221, 264, 1125
BsmI GAATGC 1 cut(s) 607
Bsp1286I GDGCHC 2 cut(s) 140, 155
Bsp143I GATC 6 cut(s) 142, 248, 274, 661, 873, 1138
Bsp68I TCGCGA 1 cut(s) 631
BspACI CCGC 1 cut(s) 17
BspCNI CTCAG 2 cut(s) 181, 1099
BspDI ATCGAT 1 cut(s) 1141
BspFNI CGCG 1 cut(s) 631
BspLI GGNNCC 2 cut(s) 814, 1053
BspMI ACCTGC 1 cut(s) 432
BsrDI GCAATG 2 cut(s) 576, 961
BssMI GATC 6 cut(s) 142, 248, 274, 661, 873, 1138
Bst4CI ACNGT 2 cut(s) 41, 157
BstAPI GCANNNNNTGC 2 cut(s) 85, 566
BstC8I GCNNGC 2 cut(s) 74, 977
BstDEI CTNAG 4 cut(s) 189, 321, 1107, 1135
BstEII GGTNACC 1 cut(s) 751
BstF5I GGATG 5 cut(s) 403, 451, 696, 799, 904
BstFNI CGCG 1 cut(s) 631
BstHHI GCGC 3 cut(s) 406, 954, 1010
BstKTI GATC 6 cut(s) 145, 251, 277, 664, 876, 1141
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 6 cut(s) 142, 248, 274, 661, 873, 1138
BstMWI GCNNNNNNNGC 3 cut(s) 85, 512, 566
BstNSI RCATGY 1 cut(s) 226
BstPI GGTNACC 1 cut(s) 751
BstSFI CTRYAG 3 cut(s) 459, 645, 921
BstUI CGCG 1 cut(s) 631
BstV1I GCAGC 5 cut(s) 88, 413, 493, 653, 662
BstV2I GAAGAC 1 cut(s) 528
Bsu15I ATCGAT 1 cut(s) 1141
BsuTUI ATCGAT 1 cut(s) 1141
BtsCI GGATG 5 cut(s) 403, 451, 696, 799, 904
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 3 cut(s) 316, 564, 848
BtuMI TCGCGA 1 cut(s) 631
BveI ACCTGC 1 cut(s) 432
Cac8I GCNNGC 2 cut(s) 74, 977
CaiI CAGNNNCTG 2 cut(s) 203, 506
CfoI GCGC 3 cut(s) 406, 954, 1010
Cfr13I GGNCC 2 cut(s) 208, 812
ClaI ATCGAT 1 cut(s) 1141
Csp6I GTAC 1 cut(s) 341
CviAII CATG 3 cut(s) 31, 223, 386
CviQI GTAC 1 cut(s) 341
DdeI CTNAG 4 cut(s) 189, 321, 1107, 1135
DpnI GATC 6 cut(s) 144, 250, 276, 663, 875, 1140
DpnII GATC 6 cut(s) 142, 248, 274, 661, 873, 1138
Ecl136II GAGCTC 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 140
Eco47I GGWCC 2 cut(s) 208, 812
Eco53kI GAGCTC 1 cut(s) 138
Eco57I CTGAAG 2 cut(s) 515, 1109
Eco91I GGTNACC 1 cut(s) 751
EcoICRI GAGCTC 1 cut(s) 138
EcoO109I RGGNCCY 1 cut(s) 812
EcoO65I GGTNACC 1 cut(s) 751
EcoRI GAATTC 1 cut(s) 163
EcoT22I ATGCAT 1 cut(s) 308
EcoT38I GRGCYC 1 cut(s) 140
FaeI CATG 3 cut(s) 34, 226, 389
FaqI GGGAC 3 cut(s) 221, 264, 1125
FatI CATG 3 cut(s) 30, 222, 385
FauNDI CATATG 1 cut(s) 285
FbaI TGATCA 1 cut(s) 873
Fnu4HI GCNGC 5 cut(s) 77, 402, 507, 642, 676
FokI GGATG 5 cut(s) 410, 458, 703, 806, 911
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 5 cut(s) 77, 402, 507, 642, 676
FspBI CTAG 1 cut(s) 1083
GlaI GCGC 3 cut(s) 405, 953, 1009
GluI GCNGC 5 cut(s) 77, 402, 507, 642, 676
GsuI CTGGAG 1 cut(s) 627
HapII CCGG 1 cut(s) 830
HhaI GCGC 3 cut(s) 406, 954, 1010
Hin1II CATG 3 cut(s) 34, 226, 389
Hin6I GCGC 3 cut(s) 404, 952, 1008
HinP1I GCGC 3 cut(s) 404, 952, 1008
HinfI GANTC 5 cut(s) 122, 194, 432, 701, 1086
HpaII CCGG 1 cut(s) 830
HphI GGTGA 7 cut(s) 142, 487, 745, 779, 812, 898, 1048
Hpy166II GTNNAC 2 cut(s) 208, 906
Hpy188I TCNGA 5 cut(s) 142, 190, 265, 735, 1091
Hpy188III TCNNGA 5 cut(s) 252, 416, 630, 779, 1083
Hpy8I GTNNAC 2 cut(s) 208, 906
HpyAV CCTTC 8 cut(s) 97, 112, 254, 373, 776, 784, 930, 1141
HpyCH4III ACNGT 2 cut(s) 41, 157
HpyCH4IV ACGT 1 cut(s) 1093
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 512, 566
HpyF3I CTNAG 4 cut(s) 189, 321, 1107, 1135
HpySE526I ACGT 1 cut(s) 1093
Hsp92II CATG 3 cut(s) 34, 226, 389
HspAI GCGC 3 cut(s) 404, 952, 1008
Ksp22I TGATCA 1 cut(s) 873
Kzo9I GATC 6 cut(s) 142, 248, 274, 661, 873, 1138
LmnI GCTCC 3 cut(s) 143, 638, 1051
Lsp1109I GCAGC 5 cut(s) 88, 413, 493, 653, 662
LweI GCATC 7 cut(s) 4, 315, 388, 436, 493, 681, 784
MaeI CTAG 1 cut(s) 1083
MaeII ACGT 1 cut(s) 1093
MaeIII GTNAC 5 cut(s) 41, 225, 311, 416, 751
MalI GATC 6 cut(s) 144, 250, 276, 663, 875, 1140
MboI GATC 6 cut(s) 142, 248, 274, 661, 873, 1138
MboII GAAGA 4 cut(s) 117, 302, 528, 1115
MhlI GDGCHC 2 cut(s) 140, 155
MluCI AATT 7 cut(s) 109, 163, 407, 696, 839, 866, 1018
MlyI GAGTC 3 cut(s) 441, 710, 1095
Mph1103I ATGCAT 1 cut(s) 308
MseI TTAA 1 cut(s) 837
MslI CAYNNNNRTG 1 cut(s) 569
MspI CCGG 1 cut(s) 830
Mva1269I GAATGC 1 cut(s) 607
MvnI CGCG 1 cut(s) 631
MwoI GCNNNNNNNGC 3 cut(s) 85, 512, 566
NdeI CATATG 1 cut(s) 285
NdeII GATC 6 cut(s) 142, 248, 274, 661, 873, 1138
NlaIII CATG 3 cut(s) 34, 226, 389
NlaIV GGNNCC 2 cut(s) 814, 1053
NmuCI GTSAC 4 cut(s) 225, 311, 416, 751
NruI TCGCGA 1 cut(s) 631
NsiI ATGCAT 1 cut(s) 308
NspI RCATGY 1 cut(s) 226
PciI ACATGT 1 cut(s) 222
PctI GAATGC 1 cut(s) 607
PfeI GAWTC 2 cut(s) 122, 194
PkrI GCNGC 5 cut(s) 78, 403, 508, 643, 677
PleI GAGTC 3 cut(s) 440, 709, 1094
PpsI GAGTC 3 cut(s) 440, 709, 1094
PpuMI RGGWCCY 1 cut(s) 812
PscI ACATGT 1 cut(s) 222
PsiI TTATAA 1 cut(s) 336
Psp124BI GAGCTC 1 cut(s) 140
Psp5II RGGWCCY 1 cut(s) 812
PspEI GGTNACC 1 cut(s) 751
PspN4I GGNNCC 2 cut(s) 814, 1053
PspPI GGNCC 2 cut(s) 208, 812
PspPPI RGGWCCY 1 cut(s) 812
PstNI CAGNNNCTG 2 cut(s) 203, 506
RruI TCGCGA 1 cut(s) 631
RsaI GTAC 1 cut(s) 342
RsaNI GTAC 1 cut(s) 341
RseI CAYNNNNRTG 1 cut(s) 569
SacI GAGCTC 1 cut(s) 140
SaqAI TTAA 1 cut(s) 837
SatI GCNGC 5 cut(s) 77, 402, 507, 642, 676
Sau3AI GATC 6 cut(s) 142, 248, 274, 661, 873, 1138
Sau96I GGNCC 2 cut(s) 208, 812
SchI GAGTC 3 cut(s) 441, 710, 1095
SduI GDGCHC 2 cut(s) 140, 155
SfaNI GCATC 7 cut(s) 4, 315, 388, 436, 493, 681, 784
SfcI CTRYAG 3 cut(s) 459, 645, 921
SinI GGWCC 2 cut(s) 208, 812
SmiMI CAYNNNNRTG 1 cut(s) 569
SmlI CTYRAG 1 cut(s) 817
SmoI CTYRAG 1 cut(s) 817
Sse9I AATT 7 cut(s) 109, 163, 407, 696, 839, 866, 1018
SsiI CCGC 1 cut(s) 17
SspMI CTAG 1 cut(s) 1083
SstI GAGCTC 1 cut(s) 140
TaaI ACNGT 2 cut(s) 41, 157
TaiI ACGT 1 cut(s) 1096
TaqI TCGA 3 cut(s) 247, 934, 1141
TasI AATT 7 cut(s) 109, 163, 407, 696, 839, 866, 1018
TatI WGTACW 1 cut(s) 340
TfiI GAWTC 2 cut(s) 122, 194
Tru1I TTAA 1 cut(s) 837
Tru9I TTAA 1 cut(s) 837
TscAI CASTG 3 cut(s) 316, 571, 855
TseFI GTSAC 4 cut(s) 225, 311, 416, 751
TseI GCWGC 5 cut(s) 76, 401, 506, 641, 675
Tsp45I GTSAC 4 cut(s) 225, 311, 416, 751
TspDTI ATGAA 2 cut(s) 366, 879
TspRI CASTG 3 cut(s) 316, 571, 855
VpaK11BI GGWCC 2 cut(s) 208, 812
XapI RAATTY 2 cut(s) 163, 866
XbaI TCTAGA 1 cut(s) 1082
XceI RCATGY 1 cut(s) 226
XspI CTAG 1 cut(s) 1083
Zsp2I ATGCAT 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.