MD02G1032800.v1.1

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014250
Physical Location & Seq
Reverse (-)
2569012 .. 2571452
2441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1032800.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGCTAATGCTTGGAAAAGAGACAAATCCATGAGACCCCTTGTGTTCCTCTTCTCTGCAACTATCGTCCTCATCTTCATCTTCTTCTTTTTCGCCACCCGCCCTTCCGCCACCCCCGCCGCCAACGACCCCACCATCCGAACCAATCTTCCAATCTACCGAATCCCGCCGTTCAATTGCGAGGAGTGCCCGCAGGCGTACCCCGTCATCGCCAACGTCGTTGAAGGCCTCCGCTATCCCTTCATCTACTCGCTCGCCGATTTGGGCAGCTTGCCGGAGAAGCCCCACAAGAACATCGTCCGATTCCTCAAGGGGAAGCCGTTTCGGAGGCCCGATATCTCGGCCACGATTCAGGGCGTTCTCGAGAAATTCAAAGGGGAAGGGAGGGACAACGGGCTGGTGGTGGACGTCGGTGCCAATGTCGGCATGGCCAGCTTCGCGGCCGCCGTCATGGGTTTTCAGGTGCTTGCTTTTGAGCCGGTTTTCGAGAATTTGCAGAGGATTTGTGATGGGATTTACTTGAATCGTGTGGGGGATTTGGTGACGGTGTTCGAGGCCGCCGCCTCCGACCGCCCTGGCAATATTACCTTCCACAAGTTGGTTGGTCGGATGGACAACAGTGCTATTTCAGCGACTGGTGCAAGGCTGGCTTTCAAGAATAACGAAGAAGTGGCAGTTCAAGTAAGAACCATTCCTCTCGATGATGTGATCCCGGAAACAGAGCCTGTTCTCCTGCTCAAAATTGATGTGCAAGGTTGGGAATATCATGTGCTGAAAGGAGCTTCCAAGTTACTGTCAAGAAAGGCAGGAGAAGCACCCTATGTCATCTATGAGGAAGATGAGCGGCTGCTGCGTGCCAGCAATAGCAGTGCTGGAGAGATTCGAAATTTCCTGAAGGGTGTGGGTTACAATCACTGCACGCTGCATGGTACAGATGCACACTGCACCAAATTGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_21

Protein Analysis

320

Amino Acids

35.26

Weight (kDa)

8.21

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_21 PF05050 136 - 303 2.4e-18 Methyltransferase FkbM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26680
fragaria_vesca FvH4_1g03280 FvH4_1g03280
malus_domestica MD02G1032800.v1.1
prunus_persica Prupe.7G242700_v2.0.a1
pyrus_communis pycom15g15480
rosa_chinensis RchiOBHm_Chr2g0088431
rosa_laevigata RLG00000015954 RLG00000015958
rosa_multiflora Rmu_sc0004733.1_g000021
rosa_roxburghii Rroxscaffold_2G00152670
rosa_rugosa Rorug01G0484000 Rorug01G0484100
rosa_samantha Rh2BG038000 Rh2CG039100 Rh2DG038700
rosa_wichuraiana Rw2G003070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 413
AccB7I CCANNNNNTGG 1 cut(s) 598
AccBSI CCGCTC 1 cut(s) 844
AccII CGCG 1 cut(s) 440
AclWI GGATC 1 cut(s) 703
AcoI YGGCCR 3 cut(s) 342, 429, 441
AcsI RAATTY 3 cut(s) 368, 490, 886
AcuI CTGAAG 1 cut(s) 914
AcyI GRCGYC 1 cut(s) 408
AfaI GTAC 2 cut(s) 200, 931
AfiI CCNNNNNNNGG 1 cut(s) 598
AgsI TTSAA 6 cut(s) 175, 224, 373, 523, 655, 680
AjnI CCWGG 1 cut(s) 574
AloI GAACNNNNNNTCC 2 cut(s) 133, 165
AluBI AGCT 3 cut(s) 270, 435, 782
AluI AGCT 3 cut(s) 270, 435, 782
Alw26I GTCTC 2 cut(s) 15, 28
AlwI GGATC 1 cut(s) 703
AlwNI CAGNNNCTG 2 cut(s) 635, 725
Ama87I CYCGRG 1 cut(s) 362
AoxI GGCC 6 cut(s) 226, 329, 342, 429, 441, 555
ApeKI GCWGC 4 cut(s) 267, 847, 850, 922
ApoI RAATTY 3 cut(s) 368, 490, 886
AspS9I GGNCC 1 cut(s) 330
AsuC2I CCSGG 1 cut(s) 713
AsuHPI GGTGA 1 cut(s) 553
AsuII TTCGAA 1 cut(s) 883
AvaI CYCGRG 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 191
BalI TGGCCA 1 cut(s) 431
BanI GGYRCC 1 cut(s) 413
BbvI GCAGC 4 cut(s) 279, 834, 837, 909
BccI CCATC 3 cut(s) 143, 503, 604
BceAI ACGGC 3 cut(s) 154, 304, 431
BciT130I CCWGG 1 cut(s) 576
BcnI CCSGG 1 cut(s) 713
BcoDI GTCTC 2 cut(s) 15, 28
Bme1390I CCNGG 2 cut(s) 576, 713
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 1 cut(s) 330
BmiI GGNNCC 1 cut(s) 415
BmrFI CCNGG 2 cut(s) 576, 713
BmsI GCATC 1 cut(s) 925
BpmI CTGGAG 1 cut(s) 894
Bpu14I TTCGAA 1 cut(s) 883
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 713
BsaHI GRCGYC 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 574
Bsc4I CCNNNNNNNGG 1 cut(s) 598
Bse118I RCCGGY 1 cut(s) 478
Bse1I ACTGG 1 cut(s) 640
BseBI CCWGG 1 cut(s) 576
BseDI CCNNGG 1 cut(s) 574
BseGI GGATG 2 cut(s) 135, 615
BseLI CCNNNNNNNGG 1 cut(s) 598
BseNI ACTGG 1 cut(s) 640
BseRI GAGGAG 1 cut(s) 197
BseSI GKGCMC 1 cut(s) 191
BseX3I CGGCCG 1 cut(s) 441
BseXI GCAGC 4 cut(s) 279, 834, 837, 909
BsgI GTGCAG 2 cut(s) 901, 928
Bsh1236I CGCG 1 cut(s) 440
Bsh1285I CGRYCG 2 cut(s) 444, 571
BshFI GGCC 6 cut(s) 228, 331, 344, 431, 443, 557
BshNI GGYRCC 1 cut(s) 413
BsiEI CGRYCG 2 cut(s) 444, 571
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 3 cut(s) 275, 479, 713
BslFI GGGAC 1 cut(s) 401
BslI CCNNNNNNNGG 1 cut(s) 598
BsmAI GTCTC 2 cut(s) 15, 28
BsmFI GGGAC 1 cut(s) 401
BsnI GGCC 6 cut(s) 228, 331, 344, 431, 443, 557
Bso31I GGTCTC 1 cut(s) 28
BsoBI CYCGRG 1 cut(s) 362
Bsp119I TTCGAA 1 cut(s) 883
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 1 cut(s) 708
BspANI GGCC 6 cut(s) 228, 331, 344, 431, 443, 557
BspFNI CGCG 1 cut(s) 440
BspLI GGNNCC 1 cut(s) 415
BspPI GGATC 1 cut(s) 703
BspT104I TTCGAA 1 cut(s) 883
BspT107I GGYRCC 1 cut(s) 413
BspTNI GGTCTC 1 cut(s) 28
BsrBI CCGCTC 1 cut(s) 844
BsrFI RCCGGY 1 cut(s) 478
BsrI ACTGG 1 cut(s) 640
BssAI RCCGGY 1 cut(s) 478
BssECI CCNNGG 1 cut(s) 574
BssMI GATC 1 cut(s) 708
BssNI GRCGYC 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 3 cut(s) 547, 620, 795
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 408
BstBI TTCGAA 1 cut(s) 883
BstF5I GGATG 2 cut(s) 135, 615
BstFNI CGCG 1 cut(s) 440
BstKTI GATC 1 cut(s) 711
BstMAI GTCTC 2 cut(s) 15, 28
BstMBI GATC 1 cut(s) 708
BstMCI CGRYCG 2 cut(s) 444, 571
BstNI CCWGG 1 cut(s) 576
BstSCI CCNGG 2 cut(s) 574, 711
BstSLI GKGCMC 1 cut(s) 191
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 4 cut(s) 279, 834, 837, 909
BstZI CGGCCG 1 cut(s) 441
BsuRI GGCC 6 cut(s) 228, 331, 344, 431, 443, 557
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 2 cut(s) 135, 615
BtsI GCAGTG 3 cut(s) 874, 913, 940
BtsIMutI CAGTG 4 cut(s) 625, 874, 913, 940
CaiI CAGNNNCTG 2 cut(s) 635, 725
CciNI GCGGCCGC 1 cut(s) 441
Cfr10I RCCGGY 1 cut(s) 478
Cfr13I GGNCC 1 cut(s) 330
Csp6I GTAC 2 cut(s) 199, 930
CviAII CATG 5 cut(s) 31, 427, 451, 767, 926
CviQI GTAC 2 cut(s) 199, 930
DpnI GATC 1 cut(s) 710
DpnII GATC 1 cut(s) 708
EaeI YGGCCR 3 cut(s) 342, 429, 441
EagI CGGCCG 1 cut(s) 441
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EciI GGCGGA 1 cut(s) 97
EclXI CGGCCG 1 cut(s) 441
Eco147I AGGCCT 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 28
Eco32I GATATC 1 cut(s) 337
Eco52I CGGCCG 1 cut(s) 441
Eco57I CTGAAG 1 cut(s) 914
Eco88I CYCGRG 1 cut(s) 362
EcoRII CCWGG 1 cut(s) 574
EcoRV GATATC 1 cut(s) 337
FaeI CATG 5 cut(s) 34, 430, 454, 770, 929
FaiI YATR 7 cut(s) 32, 428, 452, 768, 822, 831, 927
FalI AAGNNNNNCTT 2 cut(s) 634, 666
FaqI GGGAC 1 cut(s) 401
FatI CATG 5 cut(s) 30, 426, 450, 766, 925
FauI CCCGC 4 cut(s) 107, 124, 174, 198
FokI GGATG 2 cut(s) 122, 622
GsuI CTGGAG 1 cut(s) 894
HaeIII GGCC 6 cut(s) 228, 331, 344, 431, 443, 557
HapII CCGG 3 cut(s) 275, 479, 713
Hin1I GRCGYC 1 cut(s) 408
Hin1II CATG 5 cut(s) 34, 430, 454, 770, 929
HinfI GANTC 5 cut(s) 162, 303, 349, 523, 880
HpaII CCGG 3 cut(s) 275, 479, 713
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 1 cut(s) 406
Hpy188I TCNGA 5 cut(s) 140, 302, 327, 568, 609
Hpy188III TCNNGA 7 cut(s) 362, 364, 487, 655, 698, 798, 892
Hpy8I GTNNAC 1 cut(s) 406
Hpy99I CGWCG 2 cut(s) 221, 413
HpyAV CCTTC 6 cut(s) 114, 218, 250, 374, 598, 889
HpyCH4III ACNGT 3 cut(s) 547, 620, 795
HpyCH4IV ACGT 2 cut(s) 216, 408
HpyCH4V TGCA 8 cut(s) 59, 496, 641, 751, 918, 925, 938, 945
HpySE526I ACGT 2 cut(s) 216, 408
Hsp92I GRCGYC 1 cut(s) 408
Hsp92II CATG 5 cut(s) 34, 430, 454, 770, 929
Kzo9I GATC 1 cut(s) 708
LmnI GCTCC 1 cut(s) 779
Lsp1109I GCAGC 4 cut(s) 279, 834, 837, 909
LweI GCATC 1 cut(s) 925
MaeII ACGT 2 cut(s) 216, 408
MaeIII GTNAC 3 cut(s) 541, 789, 905
MalI GATC 1 cut(s) 710
MbiI CCGCTC 1 cut(s) 844
MboI GATC 1 cut(s) 708
MboII GAAGA 7 cut(s) 43, 67, 73, 76, 140, 677, 848
MfeI CAATTG 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 191
MlsI TGGCCA 1 cut(s) 431
MluCI AATT 6 cut(s) 175, 368, 490, 741, 886, 950
MluNI TGGCCA 1 cut(s) 431
MmeI TCCRAC 2 cut(s) 587, 591
Mox20I TGGCCA 1 cut(s) 431
MscI TGGCCA 1 cut(s) 431
Msp20I TGGCCA 1 cut(s) 431
MspI CCGG 3 cut(s) 275, 479, 713
MspR9I CCNGG 2 cut(s) 576, 713
MunI CAATTG 1 cut(s) 175
MvaI CCWGG 1 cut(s) 576
MvnI CGCG 1 cut(s) 440
NciI CCSGG 1 cut(s) 713
NdeII GATC 1 cut(s) 708
NlaIII CATG 5 cut(s) 34, 430, 454, 770, 929
NlaIV GGNNCC 1 cut(s) 415
NmeAIII GCCGAG 1 cut(s) 320
NmuCI GTSAC 1 cut(s) 541
NotI GCGGCCGC 1 cut(s) 441
NspV TTCGAA 1 cut(s) 883
PaeR7I CTCGAG 1 cut(s) 362
PceI AGGCCT 1 cut(s) 228
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 444
PfeI GAWTC 5 cut(s) 162, 303, 349, 523, 880
PflMI CCANNNNNTGG 1 cut(s) 598
PfoI TCCNGGA 1 cut(s) 711
Psp6I CCWGG 1 cut(s) 574
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 1 cut(s) 415
PspPI GGNCC 1 cut(s) 330
PstNI CAGNNNCTG 2 cut(s) 635, 725
RsaI GTAC 2 cut(s) 200, 931
RsaNI GTAC 2 cut(s) 199, 930
Sau3AI GATC 1 cut(s) 708
Sau96I GGNCC 1 cut(s) 330
ScrFI CCNGG 2 cut(s) 576, 713
SduI GDGCHC 1 cut(s) 191
SetI ASST 8 cut(s) 219, 272, 411, 437, 465, 590, 757, 784
SfaNI GCATC 1 cut(s) 925
Sfr274I CTCGAG 1 cut(s) 362
SfuI TTCGAA 1 cut(s) 883
SlaI CTCGAG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 308, 362
SmoI CTYRAG 2 cut(s) 308, 362
Sse9I AATT 6 cut(s) 175, 368, 490, 741, 886, 950
SseBI AGGCCT 1 cut(s) 228
SspI AATATT 1 cut(s) 583
StuI AGGCCT 1 cut(s) 228
StyD4I CCNGG 2 cut(s) 574, 711
TaaI ACNGT 3 cut(s) 547, 620, 795
TaiI ACGT 2 cut(s) 219, 411
TaqI TCGA 5 cut(s) 363, 486, 552, 699, 883
TasI AATT 6 cut(s) 175, 368, 490, 741, 886, 950
TauI GCSGC 6 cut(s) 122, 443, 446, 560, 563, 847
TfiI GAWTC 5 cut(s) 162, 303, 349, 523, 880
TscAI CASTG 4 cut(s) 625, 874, 920, 947
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 4 cut(s) 267, 847, 850, 922
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 2 cut(s) 67, 232
TspRI CASTG 4 cut(s) 625, 874, 920, 947
Van91I CCANNNNNTGG 1 cut(s) 598
XapI RAATTY 3 cut(s) 368, 490, 886
XcmI CCANNNNNNNNNTGG 2 cut(s) 424, 599
XhoI CTCGAG 1 cut(s) 362
ZraI GACGTC 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.