Rorug01G0484100

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0014250
Physical Location & Seq
Forward (+)
57086733 .. 57089601
2869 bp
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UTR
Exon/CDS
Intron
Rorug01G0484100.1

Sequence Viewer

Length: 471 bp
ATGACGACGGTGGTGAAGAGCGTGTTGCAGGCCATCAGAGAGAAAGGTCTCCGCAGTTTCTTGAGGGAGGCGAGGGAAGAAGGTTACCTGAAATGTCTAGCTGATGGAAACCTTCTGCAAACTAAAATCCACAATATCGGGGCAAGGCTTGTTGGTGTTGATAAAGTTGGGAACAAATATTATGAGAAGCTTGACACTCAATATGGAAGACACAGGTGGGTTGAATATGTGGAGAAAGGTCGCTACAACGCTTCTCAAGTGCCACCAGAATGGCATGGTTGGCTACACTACATAACAGATCATACAGGAGACGAGCTTCTGATGCTAAAGCCACAGAGGTATCACCTTGAACACAAGGAAAATTTCACTGGTGAGGGTGATGAGTTTATCTACCACTCCAAAGGGCACAGCCTCAATCCAGGGCAGAGAGACTGGACCAGGTACCAGCCTTGGGAACCAACAAGACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_21

Protein Analysis

156

Amino Acids

18.44

Weight (kDa)

8.58

Isoelectric Point (pI)

31.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUFA12 PF05071 49 - 153 2.8e-26 NADH ubiquinone oxidoreductase subunit NDUFA12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26680
fragaria_vesca FvH4_1g03280 FvH4_1g03280
malus_domestica MD02G1032800.v1.1
prunus_persica Prupe.7G242700_v2.0.a1
pyrus_communis pycom15g15480
rosa_chinensis RchiOBHm_Chr2g0088431
rosa_laevigata RLG00000015954 RLG00000015958
rosa_multiflora Rmu_sc0004733.1_g000021
rosa_roxburghii Rroxscaffold_2G00152670
rosa_rugosa Rorug01G0484000 Rorug01G0484100
rosa_samantha Rh2BG038000 Rh2CG039100 Rh2DG038700
rosa_wichuraiana Rw2G003070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 441
AciI CCGC 1 cut(s) 52
AcsI RAATTY 1 cut(s) 361
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 451
AgsI TTSAA 2 cut(s) 224, 350
AjnI CCWGG 2 cut(s) 418, 437
AluBI AGCT 3 cut(s) 101, 190, 316
AluI AGCT 3 cut(s) 101, 190, 316
Alw26I GTCTC 3 cut(s) 53, 303, 423
AoxI GGCC 1 cut(s) 30
ApoI RAATTY 1 cut(s) 361
Asp718I GGTACC 1 cut(s) 441
AspS9I GGNCC 1 cut(s) 435
AsuHPI GGTGA 4 cut(s) 25, 335, 383, 389
AvaII GGWCC 1 cut(s) 435
BaeGI GKGCMC 1 cut(s) 408
BaeI ACNNNNGTAYC 2 cut(s) 323, 356
BanI GGYRCC 1 cut(s) 441
BbsI GAAGAC 1 cut(s) 214
BccI CCATC 2 cut(s) 41, 98
BciT130I CCWGG 2 cut(s) 420, 439
BcoDI GTCTC 3 cut(s) 53, 303, 423
BfaI CTAG 1 cut(s) 98
Bme1390I CCNGG 2 cut(s) 420, 439
Bme18I GGWCC 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 2 cut(s) 443, 456
BmrFI CCNGG 2 cut(s) 420, 439
BmsI GCATC 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 214
BpuEI CTTGAG 2 cut(s) 82, 240
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse1I ACTGG 2 cut(s) 373, 437
BseBI CCWGG 2 cut(s) 420, 439
BseDI CCNNGG 2 cut(s) 419, 449
BseLI CCNNNNNNNGG 1 cut(s) 451
BseNI ACTGG 2 cut(s) 373, 437
BseSI GKGCMC 1 cut(s) 408
BshFI GGCC 1 cut(s) 32
BshNI GGYRCC 1 cut(s) 441
BslI CCNNNNNNNGG 1 cut(s) 451
BsmAI GTCTC 3 cut(s) 53, 303, 423
BsmBI CGTCTC 1 cut(s) 303
BsnI GGCC 1 cut(s) 32
Bso31I GGTCTC 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 408
Bsp143I GATC 1 cut(s) 298
BspACI CCGC 1 cut(s) 52
BspANI GGCC 1 cut(s) 32
BspLI GGNNCC 2 cut(s) 443, 456
BspQI GCTCTTC 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 441
BspTNI GGTCTC 1 cut(s) 53
BsrI ACTGG 2 cut(s) 373, 437
BssECI CCNNGG 2 cut(s) 419, 449
BssMI GATC 1 cut(s) 298
BssT1I CCWWGG 1 cut(s) 449
Bst2UI CCWGG 2 cut(s) 420, 439
Bst4CI ACNGT 1 cut(s) 10
Bst6I CTCTTC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 30
BstEII GGTNACC 1 cut(s) 83
BstKTI GATC 1 cut(s) 301
BstMAI GTCTC 3 cut(s) 53, 303, 423
BstMBI GATC 1 cut(s) 298
BstMWI GCNNNNNNNGC 2 cut(s) 280, 322
BstNI CCWGG 2 cut(s) 420, 439
BstPI GGTNACC 1 cut(s) 83
BstSCI CCNGG 2 cut(s) 418, 437
BstSLI GKGCMC 1 cut(s) 408
BstV2I GAAGAC 1 cut(s) 214
BstXI CCANNNNNNTGG 1 cut(s) 270
BsuRI GGCC 1 cut(s) 32
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 435
CsiI ACCWGGT 1 cut(s) 437
Csp6I GTAC 1 cut(s) 442
CviAII CATG 1 cut(s) 275
CviJI RGCY 9 cut(s) 32, 101, 148, 190, 283, 316, 331, 411, 448
CviKI_1 RGCY 9 cut(s) 32, 101, 148, 190, 283, 316, 331, 411, 448
CviQI GTAC 1 cut(s) 442
DpnI GATC 1 cut(s) 300
DpnII GATC 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 449
Eco31I GGTCTC 1 cut(s) 53
Eco47I GGWCC 1 cut(s) 435
Eco91I GGTNACC 1 cut(s) 83
EcoO65I GGTNACC 1 cut(s) 83
EcoRII CCWGG 2 cut(s) 418, 437
EcoT14I CCWWGG 1 cut(s) 449
ErhI CCWWGG 1 cut(s) 449
Esp3I CGTCTC 1 cut(s) 303
FaeI CATG 1 cut(s) 278
FaiI YATR 6 cut(s) 183, 204, 228, 276, 293, 303
FatI CATG 1 cut(s) 274
FspBI CTAG 1 cut(s) 98
HaeIII GGCC 1 cut(s) 32
Hin1II CATG 1 cut(s) 278
HindIII AAGCTT 1 cut(s) 188
HphI GGTGA 4 cut(s) 25, 335, 383, 389
Hpy188I TCNGA 2 cut(s) 38, 321
Hpy188III TCNNGA 1 cut(s) 61
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 74, 122
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4V TGCA 2 cut(s) 28, 118
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 322
Hsp92II CATG 1 cut(s) 278
KpnI GGTACC 1 cut(s) 445
Kzo9I GATC 1 cut(s) 298
LguI GCTCTTC 1 cut(s) 11
LweI GCATC 1 cut(s) 312
MabI ACCWGGT 1 cut(s) 437
MaeI CTAG 1 cut(s) 98
MaeIII GTNAC 1 cut(s) 83
MalI GATC 1 cut(s) 300
MboI GATC 1 cut(s) 298
MboII GAAGA 3 cut(s) 28, 89, 219
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 1 cut(s) 361
MnlI CCTC 6 cut(s) 57, 61, 66, 330, 367, 422
MslI CAYNNNNRTG 1 cut(s) 268
MspR9I CCNGG 2 cut(s) 420, 439
MvaI CCWGG 2 cut(s) 420, 439
MwoI GCNNNNNNNGC 2 cut(s) 280, 322
NdeII GATC 1 cut(s) 298
NlaIII CATG 1 cut(s) 278
NlaIV GGNNCC 2 cut(s) 443, 456
PciSI GCTCTTC 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 418, 437
PspEI GGTNACC 1 cut(s) 83
PspGI CCWGG 2 cut(s) 418, 437
PspN4I GGNNCC 2 cut(s) 443, 456
PspPI GGNCC 1 cut(s) 435
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
RseI CAYNNNNRTG 1 cut(s) 268
SapI GCTCTTC 1 cut(s) 11
Sau3AI GATC 1 cut(s) 298
Sau96I GGNCC 1 cut(s) 435
ScrFI CCNGG 2 cut(s) 420, 439
SduI GDGCHC 1 cut(s) 408
SexAI ACCWGGT 1 cut(s) 437
SfaNI GCATC 1 cut(s) 312
SinI GGWCC 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 268
SmlI CTYRAG 2 cut(s) 61, 255
SmoI CTYRAG 2 cut(s) 61, 255
Sse9I AATT 1 cut(s) 361
SsiI CCGC 1 cut(s) 52
SspI AATATT 1 cut(s) 179
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 2 cut(s) 418, 437
StyI CCWWGG 1 cut(s) 449
TaaI ACNGT 1 cut(s) 10
TasI AATT 1 cut(s) 361
TscAI CASTG 1 cut(s) 373
TspRI CASTG 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 435
XapI RAATTY 1 cut(s) 361
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.