RLG00000015958

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014250
Physical Location & Seq
Reverse (-)
2752382 .. 2753732
1351 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015958

Sequence Viewer

Length: 657 bp
ATGCTGAAGGGGAAGCCTTTCCGGCGACCCGACATTTCGAAGACGATTCAGGAGGTTTTGGGGAGGTTCAGGAGTGCAGGGAAGAACAATGGACTTGTAGTTTACGTTGGCGGCAATGTGGGCATTGCCAGCTTCGCCGCTGCCGTGATGGGCTTTCGGGTTTTTGCTTTCGAGCCGGTTTTGGAGAATCTGCAGAGGATTTGCGATGGGATTTACCTGAATCGTGTCAGGGATTTGGTCACTGTGTTTGAGGCCGCTACGTCGGACCGCCTCGGCAATATTACCTTCCATAAGTTGGTTGGTCGTCTGGACAATAGTGCTGTTTCAGCCACTGGTGCAAAGTTGGCTTTTAAGTCTAATGAAGAGATAGCAGTTCAAGTAAGGACCGTCCCTCTTGATGATGTGATTCCAGAATCTGAACCTGTTCTTGTGCTCAAAATTGATGTTCAAGGCTGGGAATATCATGTGCTGAAAGGAGATAAGAAGTTGCCATCAAGAGCTGCAAGTGAAGCACCACATCTCATTTATGAGGAAGACGGGTGTTTGTTGGAAGCCAGTAATAGCAGTAAAGTAGAGATCCGAAAGTTCCTCTCTAGTGTGGGTTATCGTCACTGCACGCAGCATGGCACAGATGCACACTGCACCAAGGAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_21

Protein Analysis

219

Amino Acids

23.93

Weight (kDa)

8.32

Isoelectric Point (pI)

32.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_21 PF05050 36 - 203 2.4e-17 Methyltransferase FkbM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26680
fragaria_vesca FvH4_1g03280 FvH4_1g03280
malus_domestica MD02G1032800.v1.1
prunus_persica Prupe.7G242700_v2.0.a1
pyrus_communis pycom15g15480
rosa_chinensis RchiOBHm_Chr2g0088431
rosa_laevigata RLG00000015954 RLG00000015958
rosa_multiflora Rmu_sc0004733.1_g000021
rosa_roxburghii Rroxscaffold_2G00152670
rosa_rugosa Rorug01G0484000 Rorug01G0484100
rosa_samantha Rh2BG038000 Rh2CG039100 Rh2DG038700
rosa_wichuraiana Rw2G003070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 295
AciI CCGC 4 cut(s) 111, 138, 255, 268
AclWI GGATC 1 cut(s) 571
AcuI CTGAAG 1 cut(s) 26
AfiI CCNNNNNNNGG 1 cut(s) 295
AgsI TTSAA 2 cut(s) 377, 449
AluBI AGCT 2 cut(s) 132, 500
AluI AGCT 2 cut(s) 132, 500
Alw21I GWGCWC 1 cut(s) 435
AlwI GGATC 1 cut(s) 571
AlwNI CAGNNNCTG 2 cut(s) 332, 416
AoxI GGCC 1 cut(s) 252
ApeKI GCWGC 3 cut(s) 140, 500, 619
Asp700I GAANNNNTTC 2 cut(s) 17, 423
AspS9I GGNCC 2 cut(s) 265, 384
AsuII TTCGAA 1 cut(s) 38
AvaII GGWCC 2 cut(s) 265, 384
BbsI GAAGAC 2 cut(s) 47, 540
Bbv12I GWGCWC 1 cut(s) 435
BbvI GCAGC 3 cut(s) 127, 487, 631
BccI CCATC 3 cut(s) 142, 200, 499
BceAI ACGGC 1 cut(s) 128
BfaI CTAG 1 cut(s) 594
BfmI CTRYAG 1 cut(s) 191
BglI GCCNNNNNGGC 1 cut(s) 22
BisI GCNGC 6 cut(s) 112, 138, 141, 255, 501, 620
BlsI GCNGC 6 cut(s) 113, 139, 142, 256, 502, 621
Bme18I GGWCC 2 cut(s) 265, 384
BmgT120I GGNCC 2 cut(s) 265, 384
BmsI GCATC 1 cut(s) 622
BpiI GAAGAC 2 cut(s) 47, 540
Bpu14I TTCGAA 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 271, 645
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse118I RCCGGY 1 cut(s) 175
Bse1I ACTGG 2 cut(s) 337, 555
Bse3DI GCAATG 2 cut(s) 121, 123
BseDI CCNNGG 2 cut(s) 271, 645
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMI GCAATG 2 cut(s) 121, 123
BseNI ACTGG 2 cut(s) 337, 555
BseXI GCAGC 3 cut(s) 127, 487, 631
BseYI CCCAGC 1 cut(s) 453
BsgI GTGCAG 3 cut(s) 96, 598, 625
BshFI GGCC 1 cut(s) 254
BsiHKAI GWGCWC 1 cut(s) 435
BsiSI CCGG 2 cut(s) 22, 176
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 374
BsnI GGCC 1 cut(s) 254
Bsp119I TTCGAA 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 1 cut(s) 576
BspACI CCGC 4 cut(s) 111, 138, 255, 268
BspANI GGCC 1 cut(s) 254
BspMAI CTGCAG 1 cut(s) 195
BspPI GGATC 1 cut(s) 571
BspT104I TTCGAA 1 cut(s) 38
BsrDI GCAATG 2 cut(s) 121, 123
BsrFI RCCGGY 1 cut(s) 175
BsrI ACTGG 2 cut(s) 337, 555
BssAI RCCGGY 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 271, 645
BssMI GATC 1 cut(s) 576
BssT1I CCWWGG 1 cut(s) 645
Bst4CI ACNGT 2 cut(s) 244, 388
Bst6I CTCTTC 1 cut(s) 357
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 2 cut(s) 130, 617
BstKTI GATC 1 cut(s) 579
BstMBI GATC 1 cut(s) 576
BstMWI GCNNNNNNNGC 8 cut(s) 22, 120, 129, 134, 326, 335, 344, 509
BstSFI CTRYAG 1 cut(s) 191
BstV1I GCAGC 3 cut(s) 127, 487, 631
BstV2I GAAGAC 2 cut(s) 47, 540
BstX2I RGATCY 1 cut(s) 576
BstYI RGATCY 1 cut(s) 576
BsuRI GGCC 1 cut(s) 254
BtgZI GCGATG 1 cut(s) 219
BtsI GCAGTG 2 cut(s) 610, 637
BtsIMutI CAGTG 4 cut(s) 240, 330, 610, 637
Cac8I GCNNGC 2 cut(s) 130, 617
CaiI CAGNNNCTG 2 cut(s) 332, 416
Cfr10I RCCGGY 1 cut(s) 175
Cfr13I GGNCC 2 cut(s) 265, 384
CpoI CGGWCCG 1 cut(s) 265
CspI CGGWCCG 1 cut(s) 265
CviAII CATG 2 cut(s) 464, 623
DpnI GATC 1 cut(s) 578
DpnII GATC 1 cut(s) 576
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 645
Eco47I GGWCC 2 cut(s) 265, 384
Eco57I CTGAAG 1 cut(s) 26
EcoT14I CCWWGG 1 cut(s) 645
ErhI CCWWGG 1 cut(s) 645
FaeI CATG 2 cut(s) 467, 626
FaiI YATR 4 cut(s) 291, 465, 528, 624
FaqI GGGAC 1 cut(s) 374
FatI CATG 2 cut(s) 463, 622
Fnu4HI GCNGC 6 cut(s) 112, 138, 141, 255, 501, 620
Fsp4HI GCNGC 6 cut(s) 112, 138, 141, 255, 501, 620
FspBI CTAG 1 cut(s) 594
GluI GCNGC 6 cut(s) 112, 138, 141, 255, 501, 620
GsaI CCCAGC 1 cut(s) 457
HaeIII GGCC 1 cut(s) 254
HapII CCGG 2 cut(s) 22, 176
Hin1II CATG 2 cut(s) 467, 626
HinfI GANTC 5 cut(s) 46, 187, 220, 406, 413
HpaII CCGG 2 cut(s) 22, 176
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 3 cut(s) 265, 418, 581
Hpy188III TCNNGA 6 cut(s) 50, 70, 308, 395, 410, 495
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 1 cut(s) 265
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 2 cut(s) 244, 388
HpyCH4IV ACGT 2 cut(s) 105, 260
HpyCH4V TGCA 7 cut(s) 77, 193, 338, 503, 615, 635, 642
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 120, 129, 134, 326, 335, 344, 509
HpySE526I ACGT 2 cut(s) 105, 260
Hsp92II CATG 2 cut(s) 467, 626
Kzo9I GATC 1 cut(s) 576
Lsp1109I GCAGC 3 cut(s) 127, 487, 631
LweI GCATC 1 cut(s) 622
MaeI CTAG 1 cut(s) 594
MaeII ACGT 2 cut(s) 105, 260
MaeIII GTNAC 2 cut(s) 238, 608
MalI GATC 1 cut(s) 578
MboI GATC 1 cut(s) 576
MboII GAAGA 4 cut(s) 52, 94, 374, 545
MflI RGATCY 1 cut(s) 576
MhlI GDGCHC 1 cut(s) 435
MluCI AATT 1 cut(s) 438
MmeI TCCRAC 2 cut(s) 243, 528
MnlI CCTC 9 cut(s) 46, 57, 189, 244, 281, 402, 523, 599, 643
MroXI GAANNNNTTC 2 cut(s) 17, 423
MseI TTAA 2 cut(s) 351, 655
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 2 cut(s) 22, 176
MwoI GCNNNNNNNGC 8 cut(s) 22, 120, 129, 134, 326, 335, 344, 509
NdeII GATC 1 cut(s) 576
NlaIII CATG 2 cut(s) 467, 626
NmeAIII GCCGAG 1 cut(s) 252
NmuCI GTSAC 2 cut(s) 238, 608
NspV TTCGAA 1 cut(s) 38
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PdmI GAANNNNTTC 2 cut(s) 17, 423
PfeI GAWTC 5 cut(s) 46, 187, 220, 406, 413
PflMI CCANNNNNTGG 1 cut(s) 295
PkrI GCNGC 6 cut(s) 113, 139, 142, 256, 502, 621
PspFI CCCAGC 1 cut(s) 453
PspPI GGNCC 2 cut(s) 265, 384
PstI CTGCAG 1 cut(s) 195
PstNI CAGNNNCTG 2 cut(s) 332, 416
PsuI RGATCY 1 cut(s) 576
Rsr2I CGGWCCG 1 cut(s) 265
RsrII CGGWCCG 1 cut(s) 265
SaqAI TTAA 2 cut(s) 351, 655
SatI GCNGC 6 cut(s) 112, 138, 141, 255, 501, 620
Sau3AI GATC 1 cut(s) 576
Sau96I GGNCC 2 cut(s) 265, 384
SduI GDGCHC 1 cut(s) 435
SetI ASST 9 cut(s) 57, 68, 108, 134, 219, 263, 287, 424, 502
SfaNI GCATC 1 cut(s) 622
SfcI CTRYAG 1 cut(s) 191
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 2 cut(s) 265, 384
Sse9I AATT 1 cut(s) 438
SsiI CCGC 4 cut(s) 111, 138, 255, 268
SspI AATATT 1 cut(s) 280
SspMI CTAG 1 cut(s) 594
StyI CCWWGG 1 cut(s) 645
TaaI ACNGT 2 cut(s) 244, 388
TaiI ACGT 2 cut(s) 108, 263
TaqI TCGA 2 cut(s) 38, 171
TasI AATT 1 cut(s) 438
TauI GCSGC 3 cut(s) 114, 140, 257
TfiI GAWTC 5 cut(s) 46, 187, 220, 406, 413
Tru1I TTAA 2 cut(s) 351, 655
Tru9I TTAA 2 cut(s) 351, 655
TscAI CASTG 4 cut(s) 247, 337, 617, 644
TseFI GTSAC 2 cut(s) 238, 608
TseI GCWGC 3 cut(s) 140, 500, 619
Tsp45I GTSAC 2 cut(s) 238, 608
TspDTI ATGAA 1 cut(s) 375
TspRI CASTG 4 cut(s) 247, 337, 617, 644
Van91I CCANNNNNTGG 1 cut(s) 295
VpaK11BI GGWCC 2 cut(s) 265, 384
XcmI CCANNNNNNNNNTGG 1 cut(s) 296
XmnI GAANNNNTTC 2 cut(s) 17, 423
XspI CTAG 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.