Rh2CG039100

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0014250
Physical Location & Seq
Reverse (-)
3115714 .. 3119578
3865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG039100.1

Sequence Viewer

Length: 978 bp
ATGGCCAATGCTTGGAAACGAGAGAAACCCATCAGACCCATCTTTCTTTTCTGCTCCGCCACTCTCTTCCTCATCTTCTTCCTCTTCTTCCTCAGCTTCCGCCCCTTTCAAAACCCTAGCCTCCTAAACCCTAACCCCATAATCCAAACCCATCTAAACGCAAACCCAATCCGCGGCCCAATCCAACCTTTCAACTGCGATCTATGCCCCCAATCCCACCCAGTAATCGCCAACGTCGTCGAGGGCCTCCAGTACCCTTTCCTCTACTCGCTCGCCGATTTGGGGAAACTGCCGGAGAAGCCCCACAAGAACATTGTAAGAATGCTGAAGGGGAAGCCTTTCCGGCGACCCGACATTTCGGAGACGATTCAGGAGGTCTTGGGGAGGTTCAGGAGTGCAGGGAAGGACAATGGGCTTGTAGTCGACGTCGGCGGCAATGTGGGCATTGCCAGCTTCGCCGCCGCAGTGATGGGCTTTCGGGTTTTTGCTTTCGAGCCGGTTTTGGAGAATCTGCAGAGGATTTGCGATGGGATTTACTTGAATCGTGTTAGGGATTTGGTCACTGTGTTTGAGGCCGCTGCGTCGGACCGCCTCGGCAATATTACCTTCCATAAGTTGGTTGGTCGTCTGGACAATAGTGCTGTTTCAGCCACTGGTGCAAAGTTGGCTTTTAAGTCTAATGAAGAGATAGCAGTTCAAGTAAGGACTGTCCCTCTTGATGATGTAATTCCAGAATTTGAACCTGTTCTTGTGCTCAAGATTGATGTTCAAGGCTGGGAATATCATGTGCTGAAAGGAGCTAAGAAGTTACTATCAAGAGCTGCAAGTGAAGCACCATATCTCATTTATGAGGAAGACGAGCGTTTGTTGGAAGCCAGTAATAGCAGTAAAGTAGAGATCCGAAAGTTCCTCTCTAGTGTGGGTTACCGTCACTGCATGCAGCATGGCACAGATGCACACTGCACCAAGGAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_21

Protein Analysis

325

Amino Acids

36.35

Weight (kDa)

8.67

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_21 PF05050 142 - 310 6.2e-19 Methyltransferase FkbM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26680
fragaria_vesca FvH4_1g03280 FvH4_1g03280
malus_domestica MD02G1032800.v1.1
prunus_persica Prupe.7G242700_v2.0.a1
pyrus_communis pycom15g15480
rosa_chinensis RchiOBHm_Chr2g0088431
rosa_laevigata RLG00000015954 RLG00000015958
rosa_multiflora Rmu_sc0004733.1_g000021
rosa_roxburghii Rroxscaffold_2G00152670
rosa_rugosa Rorug01G0484000 Rorug01G0484100
rosa_samantha Rh2BG038000 Rh2CG039100 Rh2DG038700
rosa_wichuraiana Rw2G003070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 429
AccB7I CCANNNNNTGG 2 cut(s) 12, 616
AccI GTMKAC 1 cut(s) 423
AccII CGCG 1 cut(s) 174
AciI CCGC 9 cut(s) 57, 100, 172, 174, 432, 459, 462, 576, 589
AclWI GGATC 1 cut(s) 892
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 734
AcuI CTGAAG 1 cut(s) 347
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 4 cut(s) 12, 173, 282, 616
AgsI TTSAA 6 cut(s) 110, 193, 541, 698, 740, 770
AluBI AGCT 4 cut(s) 96, 453, 800, 821
AluI AGCT 4 cut(s) 96, 453, 800, 821
Alw21I GWGCWC 1 cut(s) 756
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 1 cut(s) 892
AlwNI CAGNNNCTG 1 cut(s) 653
AoxI GGCC 4 cut(s) 3, 175, 244, 573
ApeKI GCWGC 3 cut(s) 578, 821, 940
ApoI RAATTY 1 cut(s) 734
Asp700I GAANNNNTTC 2 cut(s) 338, 744
AspS9I GGNCC 3 cut(s) 176, 244, 586
AvaII GGWCC 1 cut(s) 586
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 861
Bbv12I GWGCWC 1 cut(s) 756
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 3 cut(s) 565, 808, 952
BccI CCATC 5 cut(s) 38, 47, 159, 463, 521
BcoDI GTCTC 1 cut(s) 356
BfaI CTAG 2 cut(s) 117, 915
BfmI CTRYAG 1 cut(s) 512
BglI GCCNNNNNGGC 1 cut(s) 343
BisI GCNGC 8 cut(s) 175, 433, 459, 462, 576, 579, 822, 941
BlsI GCNGC 8 cut(s) 176, 434, 460, 463, 577, 580, 823, 942
Bme18I GGWCC 1 cut(s) 586
BmgT120I GGNCC 3 cut(s) 176, 244, 586
BmrI ACTGGG 1 cut(s) 215
BmsI GCATC 1 cut(s) 943
BmuI ACTGGG 1 cut(s) 215
BpiI GAAGAC 1 cut(s) 861
BpmI CTGGAG 1 cut(s) 233
Bpu10I CCTNAGC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 740
BsaHI GRCGYC 1 cut(s) 426
BsaJI CCNNGG 3 cut(s) 172, 592, 966
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 173, 282, 616
Bse118I RCCGGY 1 cut(s) 496
Bse1I ACTGG 4 cut(s) 221, 250, 658, 876
Bse3DI GCAATG 2 cut(s) 442, 444
BseDI CCNNGG 3 cut(s) 172, 592, 966
BseLI CCNNNNNNNGG 4 cut(s) 12, 173, 282, 616
BseMI GCAATG 2 cut(s) 442, 444
BseMII CTCAG 1 cut(s) 106
BseNI ACTGG 4 cut(s) 221, 250, 658, 876
BseXI GCAGC 3 cut(s) 565, 808, 952
BseYI CCCAGC 1 cut(s) 774
BsgI GTGCAG 2 cut(s) 417, 946
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 4 cut(s) 5, 177, 246, 575
BsiHKAI GWGCWC 1 cut(s) 756
BsiSI CCGG 3 cut(s) 293, 343, 497
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 4 cut(s) 12, 173, 282, 616
BsmAI GTCTC 1 cut(s) 356
BsmBI CGTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 695
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 4 cut(s) 5, 177, 246, 575
Bsp1286I GDGCHC 1 cut(s) 756
Bsp143I GATC 2 cut(s) 199, 897
BspACI CCGC 9 cut(s) 57, 100, 172, 174, 432, 459, 462, 576, 589
BspANI GGCC 4 cut(s) 5, 177, 246, 575
BspCNI CTCAG 1 cut(s) 105
BspFNI CGCG 1 cut(s) 174
BspMAI CTGCAG 1 cut(s) 516
BspPI GGATC 1 cut(s) 892
BsrDI GCAATG 2 cut(s) 442, 444
BsrFI RCCGGY 1 cut(s) 496
BsrI ACTGG 4 cut(s) 221, 250, 658, 876
BssAI RCCGGY 1 cut(s) 496
BssECI CCNNGG 3 cut(s) 172, 592, 966
BssMI GATC 2 cut(s) 199, 897
BssNI GRCGYC 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 966
Bst4CI ACNGT 3 cut(s) 565, 709, 929
Bst6I CTCTTC 3 cut(s) 71, 89, 678
BstACI GRCGYC 1 cut(s) 426
BstC8I GCNNGC 3 cut(s) 273, 451, 938
BstDEI CTNAG 2 cut(s) 92, 801
BstDSI CCRYGG 1 cut(s) 172
BstEII GGTNACC 1 cut(s) 923
BstFNI CGCG 1 cut(s) 174
BstKTI GATC 2 cut(s) 202, 900
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 2 cut(s) 199, 897
BstNSI RCATGY 1 cut(s) 940
BstPI GGTNACC 1 cut(s) 923
BstSFI CTRYAG 1 cut(s) 512
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 3 cut(s) 565, 808, 952
BstV2I GAAGAC 1 cut(s) 861
BstX2I RGATCY 1 cut(s) 897
BstYI RGATCY 1 cut(s) 897
BsuRI GGCC 4 cut(s) 5, 177, 246, 575
BtgI CCRYGG 1 cut(s) 172
BtgZI GCGATG 1 cut(s) 540
BtsI GCAGTG 3 cut(s) 471, 931, 958
BtsIMutI CAGTG 5 cut(s) 471, 561, 651, 931, 958
Cac8I GCNNGC 3 cut(s) 273, 451, 938
CaiI CAGNNNCTG 1 cut(s) 653
Cfr10I RCCGGY 1 cut(s) 496
Cfr13I GGNCC 3 cut(s) 176, 244, 586
Cfr42I CCGCGG 1 cut(s) 175
CpoI CGGWCCG 1 cut(s) 586
CseI GACGC 1 cut(s) 570
Csp6I GTAC 1 cut(s) 253
CspI CGGWCCG 1 cut(s) 586
CviAII CATG 3 cut(s) 785, 937, 944
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 2 cut(s) 92, 801
DpnI GATC 2 cut(s) 201, 899
DpnII GATC 2 cut(s) 199, 897
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 71, 89, 678
EarI CTCTTC 3 cut(s) 71, 89, 678
EciI GGCGGA 2 cut(s) 46, 89
Eco130I CCWWGG 1 cut(s) 966
Eco47I GGWCC 1 cut(s) 586
Eco57I CTGAAG 1 cut(s) 347
Eco91I GGTNACC 1 cut(s) 923
EcoO109I RGGNCCY 1 cut(s) 244
EcoO65I GGTNACC 1 cut(s) 923
EcoT14I CCWWGG 1 cut(s) 966
ErhI CCWWGG 1 cut(s) 966
Esp3I CGTCTC 1 cut(s) 356
FaeI CATG 3 cut(s) 788, 940, 947
FaiI YATR 8 cut(s) 140, 205, 612, 786, 838, 849, 938, 945
FaqI GGGAC 1 cut(s) 695
FatI CATG 3 cut(s) 784, 936, 943
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 8 cut(s) 175, 433, 459, 462, 576, 579, 822, 941
Fsp4HI GCNGC 8 cut(s) 175, 433, 459, 462, 576, 579, 822, 941
FspBI CTAG 2 cut(s) 117, 915
GluI GCNGC 8 cut(s) 175, 433, 459, 462, 576, 579, 822, 941
GsaI CCCAGC 1 cut(s) 778
GsuI CTGGAG 1 cut(s) 233
HaeIII GGCC 4 cut(s) 5, 177, 246, 575
HapII CCGG 3 cut(s) 293, 343, 497
HgaI GACGC 1 cut(s) 570
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 3 cut(s) 788, 940, 947
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 3 cut(s) 367, 508, 541
HpaII CCGG 3 cut(s) 293, 343, 497
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 4 cut(s) 35, 361, 586, 902
Hpy188III TCNNGA 7 cut(s) 371, 391, 629, 716, 731, 757, 816
Hpy8I GTNNAC 1 cut(s) 424
Hpy99I CGWCG 5 cut(s) 239, 242, 428, 431, 586
HpyAV CCTTC 3 cut(s) 322, 397, 616
HpyCH4III ACNGT 3 cut(s) 565, 709, 929
HpyCH4IV ACGT 2 cut(s) 234, 426
HpyCH4V TGCA 8 cut(s) 398, 514, 659, 824, 936, 940, 956, 963
HpyF3I CTNAG 2 cut(s) 92, 801
HpySE526I ACGT 2 cut(s) 234, 426
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 3 cut(s) 788, 940, 947
KspI CCGCGG 1 cut(s) 175
Kzo9I GATC 2 cut(s) 199, 897
LmnI GCTCC 2 cut(s) 59, 797
Lsp1109I GCAGC 3 cut(s) 565, 808, 952
LweI GCATC 1 cut(s) 943
MaeI CTAG 2 cut(s) 117, 915
MaeII ACGT 2 cut(s) 234, 426
MaeIII GTNAC 4 cut(s) 559, 807, 923, 929
MalI GATC 2 cut(s) 201, 899
MboI GATC 2 cut(s) 199, 897
MboII GAAGA 7 cut(s) 58, 67, 70, 76, 79, 695, 866
MflI RGATCY 1 cut(s) 897
MhlI GDGCHC 1 cut(s) 756
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 726, 734
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 208, 564, 849
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 338, 744
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 672, 976
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 174, 578
MspI CCGG 3 cut(s) 293, 343, 497
Mva1269I GAATGC 1 cut(s) 327
MvnI CGCG 1 cut(s) 174
NdeII GATC 2 cut(s) 199, 897
NlaIII CATG 3 cut(s) 788, 940, 947
NmeAIII GCCGAG 1 cut(s) 573
NmuCI GTSAC 2 cut(s) 559, 929
NspI RCATGY 1 cut(s) 940
PaeI GCATGC 1 cut(s) 940
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PctI GAATGC 1 cut(s) 327
PdmI GAANNNNTTC 2 cut(s) 338, 744
PfeI GAWTC 3 cut(s) 367, 508, 541
PflMI CCANNNNNTGG 2 cut(s) 12, 616
PkrI GCNGC 8 cut(s) 176, 434, 460, 463, 577, 580, 823, 942
PspEI GGTNACC 1 cut(s) 923
PspFI CCCAGC 1 cut(s) 774
PspPI GGNCC 3 cut(s) 176, 244, 586
PstI CTGCAG 1 cut(s) 516
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 897
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
Rsr2I CGGWCCG 1 cut(s) 586
RsrII CGGWCCG 1 cut(s) 586
SacII CCGCGG 1 cut(s) 175
SalI GTCGAC 1 cut(s) 422
SaqAI TTAA 2 cut(s) 672, 976
SatI GCNGC 8 cut(s) 175, 433, 459, 462, 576, 579, 822, 941
Sau3AI GATC 2 cut(s) 199, 897
Sau96I GGNCC 3 cut(s) 176, 244, 586
SduI GDGCHC 1 cut(s) 756
SfaNI GCATC 1 cut(s) 943
SfcI CTRYAG 1 cut(s) 512
Sfr303I CCGCGG 1 cut(s) 175
SgrBI CCGCGG 1 cut(s) 175
SinI GGWCC 1 cut(s) 586
SmlI CTYRAG 1 cut(s) 755
SmoI CTYRAG 1 cut(s) 755
SphI GCATGC 1 cut(s) 940
Sse9I AATT 2 cut(s) 726, 734
SsiI CCGC 9 cut(s) 57, 100, 172, 174, 432, 459, 462, 576, 589
SspI AATATT 1 cut(s) 601
SspMI CTAG 2 cut(s) 117, 915
StyI CCWWGG 1 cut(s) 966
TaaI ACNGT 3 cut(s) 565, 709, 929
TaiI ACGT 2 cut(s) 237, 429
TaqI TCGA 3 cut(s) 240, 423, 492
TasI AATT 2 cut(s) 726, 734
TauI GCSGC 5 cut(s) 177, 435, 461, 464, 578
TfiI GAWTC 3 cut(s) 367, 508, 541
Tru1I TTAA 2 cut(s) 672, 976
Tru9I TTAA 2 cut(s) 672, 976
TscAI CASTG 5 cut(s) 471, 568, 658, 938, 965
TseFI GTSAC 2 cut(s) 559, 929
TseI GCWGC 3 cut(s) 578, 821, 940
Tsp45I GTSAC 2 cut(s) 559, 929
TspDTI ATGAA 1 cut(s) 696
TspRI CASTG 5 cut(s) 471, 568, 658, 938, 965
Van91I CCANNNNNTGG 2 cut(s) 12, 616
VpaK11BI GGWCC 1 cut(s) 586
XapI RAATTY 1 cut(s) 734
XceI RCATGY 1 cut(s) 940
XcmI CCANNNNNNNNNTGG 1 cut(s) 617
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 2 cut(s) 338, 744
XspI CTAG 2 cut(s) 117, 915
ZraI GACGTC 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.