Rroxscaffold_2G00152670

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014250
Physical Location & Seq
Forward (+)
89552429 .. 89555296
2868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152670.1

Sequence Viewer

Length: 981 bp
ATGGCCAATGCTTGGAAACGAGAGAAACCCATCAGACCCATCTTTCTTTTCTGCTCCGCCACTCTCTTCCTCATCTTCTTCCTCTTCTTCCTCAGCTTCCGCCCCTTTCAAAACCCTAGCCTCCTAAACCCTAACCCCATAATCCAAACCCATCTAAACCCAAACCCAATCCGCGGCCCAATCCAACCTTTCAACTGCGATCTATGCCCCCAATCCCACCCAGTAATCGCCAACGTCGTCGAGGGCCTCCGGTACCCTTTCCTCTACTCGCTTGCCGATTTGGGGAAACTGCCGGAGAAGCCCCACAAGAACATTGTGAGAATGCTGAAGGGGAAGCCTTTCCGGCGACCCGACATTTCGGAGACGATTCAGGAGGTCTTGGGGAGGTTCAGGAGTGCAGGGAAGGACAATGGGCTTGTAGTCGACGTCGGCGGCAATGTGGGCATTGCCAGCTTCGCCGCCGCCGCCGTGATGGGCTTTCGGGTTTTTGCTTTCGAGCCGGTTTTGGAGAATCTGCAGAGGATTTGCGATGGGATTTACTTGAATCGTGTTAGGGATTTGGTCACTGTGTTTGAGGCCGCTGCGTCGGACCGCCTCGGCAATATTACCTTCCATAAGTTGGTTGGTCGTCTGGACAATAGTGCTGTTTCAGCCACTGGTGCAAAGTTGGCTTTTAAGTCTAATGAAGAGATAGCAGTTCAAGTAAGGACTGTCCCTCTTGATGATGTAATTCCAGAATTTGAACCTGTTCTTCTGCTCAAGATTGATGTCCAAGGCTGGGAATATCATGTGCTGAAAGGAGCTAAGAAGTTACTATCAAGAGCTGCAAGTGAAGCACCATATCTCATTTATGAGGAAGACGAGCGTTTGTTGGAAGCCAGTAATAGCAGTAAAGTAGAGATCCGAAAGTTCCTCTCTAGTGTGGGTTACCGTCACTGCACGCAGCATGGCACAGATGCACACTGCACCAAGGATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_21

Protein Analysis

326

Amino Acids

36.44

Weight (kDa)

8.83

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_21 PF05050 142 - 311 1.4e-19 Methyltransferase FkbM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26680
fragaria_vesca FvH4_1g03280 FvH4_1g03280
malus_domestica MD02G1032800.v1.1
prunus_persica Prupe.7G242700_v2.0.a1
pyrus_communis pycom15g15480
rosa_chinensis RchiOBHm_Chr2g0088431
rosa_laevigata RLG00000015954 RLG00000015958
rosa_multiflora Rmu_sc0004733.1_g000021
rosa_roxburghii Rroxscaffold_2G00152670
rosa_rugosa Rorug01G0484000 Rorug01G0484100
rosa_samantha Rh2BG038000 Rh2CG039100 Rh2DG038700
rosa_wichuraiana Rw2G003070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 429
Acc65I GGTACC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 252
AccB7I CCANNNNNTGG 2 cut(s) 12, 619
AccI GTMKAC 1 cut(s) 423
AccII CGCG 1 cut(s) 174
AclWI GGATC 1 cut(s) 895
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 737
AcuI CTGAAG 1 cut(s) 347
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 5 cut(s) 12, 173, 282, 619, 778
AgsI TTSAA 5 cut(s) 110, 193, 544, 701, 743
AluBI AGCT 4 cut(s) 96, 453, 803, 824
AluI AGCT 4 cut(s) 96, 453, 803, 824
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 1 cut(s) 895
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 4 cut(s) 3, 175, 244, 576
ApeKI GCWGC 3 cut(s) 581, 824, 943
ApoI RAATTY 1 cut(s) 737
Asp700I GAANNNNTTC 2 cut(s) 338, 747
Asp718I GGTACC 1 cut(s) 252
AspS9I GGNCC 3 cut(s) 176, 244, 589
AvaII GGWCC 1 cut(s) 589
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 864
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 3 cut(s) 568, 811, 955
BccI CCATC 6 cut(s) 38, 47, 159, 466, 524, 968
BceAI ACGGC 1 cut(s) 452
BcoDI GTCTC 1 cut(s) 356
BfaI CTAG 2 cut(s) 117, 918
BfmI CTRYAG 1 cut(s) 515
BglI GCCNNNNNGGC 1 cut(s) 343
BisI GCNGC 9 cut(s) 175, 433, 459, 462, 465, 579, 582, 825, 944
BlsI GCNGC 9 cut(s) 176, 434, 460, 463, 466, 580, 583, 826, 945
Bme18I GGWCC 1 cut(s) 589
BmgT120I GGNCC 3 cut(s) 176, 244, 589
BmiI GGNNCC 1 cut(s) 254
BmrI ACTGGG 1 cut(s) 215
BmsI GCATC 1 cut(s) 946
BmuI ACTGGG 1 cut(s) 215
BpiI GAAGAC 1 cut(s) 864
Bpu10I CCTNAGC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 743
BsaHI GRCGYC 1 cut(s) 426
BsaJI CCNNGG 4 cut(s) 172, 595, 772, 969
BsaWI WCCGGW 1 cut(s) 249
Bsc4I CCNNNNNNNGG 5 cut(s) 12, 173, 282, 619, 778
Bse118I RCCGGY 1 cut(s) 499
Bse1I ACTGG 3 cut(s) 221, 661, 879
Bse3DI GCAATG 2 cut(s) 442, 444
BseDI CCNNGG 4 cut(s) 172, 595, 772, 969
BseGI GGATG 1 cut(s) 979
BseLI CCNNNNNNNGG 5 cut(s) 12, 173, 282, 619, 778
BseMI GCAATG 2 cut(s) 442, 444
BseMII CTCAG 1 cut(s) 106
BseNI ACTGG 3 cut(s) 221, 661, 879
BseXI GCAGC 3 cut(s) 568, 811, 955
BseYI CCCAGC 1 cut(s) 777
BsgI GTGCAG 3 cut(s) 417, 922, 949
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 4 cut(s) 5, 177, 246, 578
BshNI GGYRCC 1 cut(s) 252
BsiSI CCGG 4 cut(s) 250, 293, 343, 500
BslFI GGGAC 1 cut(s) 698
BslI CCNNNNNNNGG 5 cut(s) 12, 173, 282, 619, 778
BsmAI GTCTC 1 cut(s) 356
BsmBI CGTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 698
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 4 cut(s) 5, 177, 246, 578
Bsp143I GATC 2 cut(s) 199, 900
BspANI GGCC 4 cut(s) 5, 177, 246, 578
BspCNI CTCAG 1 cut(s) 105
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 1 cut(s) 254
BspMAI CTGCAG 1 cut(s) 519
BspPI GGATC 1 cut(s) 895
BspT107I GGYRCC 1 cut(s) 252
BsrDI GCAATG 2 cut(s) 442, 444
BsrFI RCCGGY 1 cut(s) 499
BsrI ACTGG 3 cut(s) 221, 661, 879
BssAI RCCGGY 1 cut(s) 499
BssECI CCNNGG 4 cut(s) 172, 595, 772, 969
BssMI GATC 2 cut(s) 199, 900
BssNI GRCGYC 1 cut(s) 426
BssT1I CCWWGG 2 cut(s) 772, 969
Bst4CI ACNGT 3 cut(s) 568, 712, 932
Bst6I CTCTTC 3 cut(s) 71, 89, 681
BstACI GRCGYC 1 cut(s) 426
BstC8I GCNNGC 3 cut(s) 273, 451, 941
BstDEI CTNAG 2 cut(s) 92, 804
BstDSI CCRYGG 1 cut(s) 172
BstEII GGTNACC 1 cut(s) 926
BstF5I GGATG 1 cut(s) 979
BstFNI CGCG 1 cut(s) 174
BstKTI GATC 2 cut(s) 202, 903
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 2 cut(s) 199, 900
BstPI GGTNACC 1 cut(s) 926
BstSFI CTRYAG 1 cut(s) 515
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 3 cut(s) 568, 811, 955
BstV2I GAAGAC 1 cut(s) 864
BstX2I RGATCY 1 cut(s) 900
BstYI RGATCY 1 cut(s) 900
BsuRI GGCC 4 cut(s) 5, 177, 246, 578
BtgI CCRYGG 1 cut(s) 172
BtgZI GCGATG 1 cut(s) 543
BtsCI GGATG 1 cut(s) 979
BtsI GCAGTG 2 cut(s) 934, 961
BtsIMutI CAGTG 4 cut(s) 564, 654, 934, 961
Cac8I GCNNGC 3 cut(s) 273, 451, 941
CaiI CAGNNNCTG 1 cut(s) 656
Cfr10I RCCGGY 1 cut(s) 499
Cfr13I GGNCC 3 cut(s) 176, 244, 589
Cfr42I CCGCGG 1 cut(s) 175
CpoI CGGWCCG 1 cut(s) 589
CseI GACGC 1 cut(s) 573
Csp6I GTAC 1 cut(s) 253
CspI CGGWCCG 1 cut(s) 589
CviAII CATG 2 cut(s) 788, 947
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 2 cut(s) 92, 804
DpnI GATC 2 cut(s) 201, 902
DpnII GATC 2 cut(s) 199, 900
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 71, 89, 681
EarI CTCTTC 3 cut(s) 71, 89, 681
EciI GGCGGA 2 cut(s) 46, 89
Eco130I CCWWGG 2 cut(s) 772, 969
Eco47I GGWCC 1 cut(s) 589
Eco57I CTGAAG 1 cut(s) 347
Eco91I GGTNACC 1 cut(s) 926
EcoO109I RGGNCCY 1 cut(s) 244
EcoO65I GGTNACC 1 cut(s) 926
EcoT14I CCWWGG 2 cut(s) 772, 969
ErhI CCWWGG 2 cut(s) 772, 969
Esp3I CGTCTC 1 cut(s) 356
FaeI CATG 2 cut(s) 791, 950
FaiI YATR 7 cut(s) 140, 205, 615, 789, 841, 852, 948
FaqI GGGAC 1 cut(s) 698
FatI CATG 2 cut(s) 787, 946
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 9 cut(s) 175, 433, 459, 462, 465, 579, 582, 825, 944
Fsp4HI GCNGC 9 cut(s) 175, 433, 459, 462, 465, 579, 582, 825, 944
FspBI CTAG 2 cut(s) 117, 918
GluI GCNGC 9 cut(s) 175, 433, 459, 462, 465, 579, 582, 825, 944
GsaI CCCAGC 1 cut(s) 781
HaeIII GGCC 4 cut(s) 5, 177, 246, 578
HapII CCGG 4 cut(s) 250, 293, 343, 500
HgaI GACGC 1 cut(s) 573
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 2 cut(s) 791, 950
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 3 cut(s) 367, 511, 544
HpaII CCGG 4 cut(s) 250, 293, 343, 500
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 4 cut(s) 35, 361, 589, 905
Hpy188III TCNNGA 7 cut(s) 371, 391, 632, 719, 734, 760, 819
Hpy8I GTNNAC 1 cut(s) 424
Hpy99I CGWCG 5 cut(s) 239, 242, 428, 431, 589
HpyAV CCTTC 3 cut(s) 322, 397, 619
HpyCH4III ACNGT 3 cut(s) 568, 712, 932
HpyCH4IV ACGT 2 cut(s) 234, 426
HpyCH4V TGCA 7 cut(s) 398, 517, 662, 827, 939, 959, 966
HpyF3I CTNAG 2 cut(s) 92, 804
HpySE526I ACGT 2 cut(s) 234, 426
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 2 cut(s) 791, 950
KpnI GGTACC 1 cut(s) 256
KspI CCGCGG 1 cut(s) 175
Kzo9I GATC 2 cut(s) 199, 900
LmnI GCTCC 2 cut(s) 59, 800
Lsp1109I GCAGC 3 cut(s) 568, 811, 955
LweI GCATC 1 cut(s) 946
MaeI CTAG 2 cut(s) 117, 918
MaeII ACGT 2 cut(s) 234, 426
MaeIII GTNAC 4 cut(s) 562, 810, 926, 932
MalI GATC 2 cut(s) 201, 902
MboI GATC 2 cut(s) 199, 900
MboII GAAGA 8 cut(s) 58, 67, 70, 76, 79, 698, 743, 869
MflI RGATCY 1 cut(s) 900
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 729, 737
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 208, 567, 852
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 338, 747
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 675, 979
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 174, 581
MspI CCGG 4 cut(s) 250, 293, 343, 500
Mva1269I GAATGC 1 cut(s) 327
MvnI CGCG 1 cut(s) 174
NdeII GATC 2 cut(s) 199, 900
NlaIII CATG 2 cut(s) 791, 950
NlaIV GGNNCC 1 cut(s) 254
NmeAIII GCCGAG 1 cut(s) 576
NmuCI GTSAC 2 cut(s) 562, 932
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PctI GAATGC 1 cut(s) 327
PdmI GAANNNNTTC 2 cut(s) 338, 747
PfeI GAWTC 3 cut(s) 367, 511, 544
PflMI CCANNNNNTGG 2 cut(s) 12, 619
PkrI GCNGC 9 cut(s) 176, 434, 460, 463, 466, 580, 583, 826, 945
PspEI GGTNACC 1 cut(s) 926
PspFI CCCAGC 1 cut(s) 777
PspN4I GGNNCC 1 cut(s) 254
PspPI GGNCC 3 cut(s) 176, 244, 589
PstI CTGCAG 1 cut(s) 519
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 900
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
Rsr2I CGGWCCG 1 cut(s) 589
RsrII CGGWCCG 1 cut(s) 589
SacII CCGCGG 1 cut(s) 175
SalI GTCGAC 1 cut(s) 422
SaqAI TTAA 2 cut(s) 675, 979
SatI GCNGC 9 cut(s) 175, 433, 459, 462, 465, 579, 582, 825, 944
Sau3AI GATC 2 cut(s) 199, 900
Sau96I GGNCC 3 cut(s) 176, 244, 589
SfaNI GCATC 1 cut(s) 946
SfcI CTRYAG 1 cut(s) 515
Sfr303I CCGCGG 1 cut(s) 175
SgrBI CCGCGG 1 cut(s) 175
SinI GGWCC 1 cut(s) 589
SmlI CTYRAG 1 cut(s) 758
SmoI CTYRAG 1 cut(s) 758
Sse9I AATT 2 cut(s) 729, 737
SspI AATATT 1 cut(s) 604
SspMI CTAG 2 cut(s) 117, 918
StyI CCWWGG 2 cut(s) 772, 969
TaaI ACNGT 3 cut(s) 568, 712, 932
TaiI ACGT 2 cut(s) 237, 429
TaqI TCGA 3 cut(s) 240, 423, 495
TasI AATT 2 cut(s) 729, 737
TauI GCSGC 6 cut(s) 177, 435, 461, 464, 467, 581
TfiI GAWTC 3 cut(s) 367, 511, 544
Tru1I TTAA 2 cut(s) 675, 979
Tru9I TTAA 2 cut(s) 675, 979
TscAI CASTG 4 cut(s) 571, 661, 941, 968
TseFI GTSAC 2 cut(s) 562, 932
TseI GCWGC 3 cut(s) 581, 824, 943
Tsp45I GTSAC 2 cut(s) 562, 932
TspDTI ATGAA 1 cut(s) 699
TspRI CASTG 4 cut(s) 571, 661, 941, 968
Van91I CCANNNNNTGG 2 cut(s) 12, 619
VpaK11BI GGWCC 1 cut(s) 589
XapI RAATTY 1 cut(s) 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 2 cut(s) 338, 747
XspI CTAG 2 cut(s) 117, 918
ZraI GACGTC 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.