Rh2BG038000

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014250
Physical Location & Seq
Reverse (-)
2643493 .. 2645663
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG038000.1

Sequence Viewer

Length: 984 bp
ATGGCCAATGCTTGGAAACGAGAGAAACCCATCAGACCCATCTTTCTTTTCTGCTCCGCCACTCTCTTCCTCATCTTCTTCCTCTTCTTCCTCAGCTTCCGCCCCTTTCAAAACCCTAGCCTCCTAAACCCTAACCCCATAATCCAAACCCATCTAAACCCAAACCCAAACCCAATCCGCGGCCCAATCCAACCTTTCAACTGCGATCTATGCCCCCAATCCCACCCAGTAATCGCCAACGTCGTCGAGGGCCTCCAGTACCCTTTCCTCTACTCGCTCGCCGATTTGGGGAAACTGCCGGAGAAGCCCCACAAGAACATTGTGAGAATGCTGAAGGGGAAGCCTTTCCGGCGACCCGACATTTCGGAGACGATTCAGGAGGTCTTGGGGAGGTTCAGGAGTGCAGGGAAGGCCAATGGGCTTGTAGTCGACGTCGGCGGCAATGTGGGCATTGCCAGCTTCGCCGCCGCCGTGATGGGCTTTCGGGTTTTTGCTTTCGAGCCGGTTTTGGAGAATCTGCAGAGGATTTGCGATGGGATTTACTTGAATCGTGTTAGGGATTTGGTCACTGTGTTTGAGGCCGCTGCGTCGGACCGCCTCGGCAATATTACCTTCCATAAGTTGGTTGGTCGTCTGGACAATAGTGCTGTTTCAGCCACTGGTGCAAAGTTGGCTTTTAAGTCTAATGAAGAGATAGCAGTTCAAGTAAGGACTGTCCCTCTTGATGATGTAATTCCAGAATTTGAACCTGTTCTTGTGCTCAAGATTGATGTTCAAGGCTGGGAATATCATGTGCTGAAAGGAGCTAAGAAGTTACTATCAAGAGCTGCAAGTGAAGCACCATATCTCATTTATGAGGAAGACGAGCGATTGTTGGAAGCTAGTAATAGCAGTAAAGTAGAGATCAGAAAGTTCCTTTCTAGTGTGGGTTACCGTCACTGTACGCAGCATGGCACAGATGCACACTGCACCAAGGAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_21

Protein Analysis

327

Amino Acids

36.51

Weight (kDa)

8.83

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_21 PF05050 144 - 312 6.3e-19 Methyltransferase FkbM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26680
fragaria_vesca FvH4_1g03280 FvH4_1g03280
malus_domestica MD02G1032800.v1.1
prunus_persica Prupe.7G242700_v2.0.a1
pyrus_communis pycom15g15480
rosa_chinensis RchiOBHm_Chr2g0088431
rosa_laevigata RLG00000015954 RLG00000015958
rosa_multiflora Rmu_sc0004733.1_g000021
rosa_roxburghii Rroxscaffold_2G00152670
rosa_rugosa Rorug01G0484000 Rorug01G0484100
rosa_samantha Rh2BG038000 Rh2CG039100 Rh2DG038700
rosa_wichuraiana Rw2G003070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 435
AccB7I CCANNNNNTGG 2 cut(s) 12, 622
AccI GTMKAC 1 cut(s) 429
AccII CGCG 1 cut(s) 180
AciI CCGC 9 cut(s) 57, 100, 178, 180, 438, 465, 468, 582, 595
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 740
AcuI CTGAAG 1 cut(s) 353
AcyI GRCGYC 1 cut(s) 432
AfaI GTAC 2 cut(s) 260, 943
AfiI CCNNNNNNNGG 4 cut(s) 12, 179, 288, 622
AgsI TTSAA 6 cut(s) 110, 199, 547, 704, 746, 776
AluBI AGCT 5 cut(s) 96, 459, 806, 827, 881
AluI AGCT 5 cut(s) 96, 459, 806, 827, 881
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 1 cut(s) 362
AlwNI CAGNNNCTG 1 cut(s) 659
AoxI GGCC 5 cut(s) 3, 181, 250, 411, 579
ApeKI GCWGC 3 cut(s) 584, 827, 946
ApoI RAATTY 1 cut(s) 740
Asp700I GAANNNNTTC 2 cut(s) 344, 750
AspS9I GGNCC 3 cut(s) 182, 250, 592
AvaII GGWCC 1 cut(s) 592
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 867
Bbv12I GWGCWC 1 cut(s) 762
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 3 cut(s) 571, 814, 958
BccI CCATC 5 cut(s) 38, 47, 159, 469, 527
BceAI ACGGC 1 cut(s) 455
BcoDI GTCTC 1 cut(s) 362
BfaI CTAG 3 cut(s) 117, 882, 921
BfmI CTRYAG 1 cut(s) 518
BglI GCCNNNNNGGC 1 cut(s) 349
BisI GCNGC 8 cut(s) 181, 439, 465, 468, 582, 585, 828, 947
BlsI GCNGC 8 cut(s) 182, 440, 466, 469, 583, 586, 829, 948
Bme18I GGWCC 1 cut(s) 592
BmgT120I GGNCC 3 cut(s) 182, 250, 592
BmrI ACTGGG 1 cut(s) 221
BmsI GCATC 1 cut(s) 949
BmuI ACTGGG 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 867
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 746
BsaHI GRCGYC 1 cut(s) 432
BsaJI CCNNGG 3 cut(s) 178, 598, 972
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 179, 288, 622
Bse118I RCCGGY 1 cut(s) 502
Bse1I ACTGG 3 cut(s) 227, 256, 664
Bse3DI GCAATG 2 cut(s) 448, 450
BseDI CCNNGG 3 cut(s) 178, 598, 972
BseLI CCNNNNNNNGG 4 cut(s) 12, 179, 288, 622
BseMI GCAATG 2 cut(s) 448, 450
BseMII CTCAG 1 cut(s) 106
BseNI ACTGG 3 cut(s) 227, 256, 664
BseXI GCAGC 3 cut(s) 571, 814, 958
BseYI CCCAGC 1 cut(s) 780
BsgI GTGCAG 2 cut(s) 423, 952
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 5 cut(s) 5, 183, 252, 413, 581
BsiHKAI GWGCWC 1 cut(s) 762
BsiSI CCGG 3 cut(s) 299, 349, 503
BslFI GGGAC 1 cut(s) 701
BslI CCNNNNNNNGG 4 cut(s) 12, 179, 288, 622
BsmAI GTCTC 1 cut(s) 362
BsmBI CGTCTC 1 cut(s) 362
BsmFI GGGAC 1 cut(s) 701
BsmI GAATGC 1 cut(s) 333
BsnI GGCC 5 cut(s) 5, 183, 252, 413, 581
Bsp1286I GDGCHC 1 cut(s) 762
Bsp143I GATC 2 cut(s) 205, 903
BspACI CCGC 9 cut(s) 57, 100, 178, 180, 438, 465, 468, 582, 595
BspANI GGCC 5 cut(s) 5, 183, 252, 413, 581
BspCNI CTCAG 1 cut(s) 105
BspFNI CGCG 1 cut(s) 180
BspMAI CTGCAG 1 cut(s) 522
BsrDI GCAATG 2 cut(s) 448, 450
BsrFI RCCGGY 1 cut(s) 502
BsrI ACTGG 3 cut(s) 227, 256, 664
BssAI RCCGGY 1 cut(s) 502
BssECI CCNNGG 3 cut(s) 178, 598, 972
BssMI GATC 2 cut(s) 205, 903
BssNI GRCGYC 1 cut(s) 432
BssT1I CCWWGG 1 cut(s) 972
Bst4CI ACNGT 4 cut(s) 571, 715, 935, 941
Bst6I CTCTTC 3 cut(s) 71, 89, 684
BstACI GRCGYC 1 cut(s) 432
BstC8I GCNNGC 2 cut(s) 279, 457
BstDEI CTNAG 2 cut(s) 92, 807
BstDSI CCRYGG 1 cut(s) 178
BstEII GGTNACC 1 cut(s) 929
BstFNI CGCG 1 cut(s) 180
BstKTI GATC 2 cut(s) 208, 906
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 2 cut(s) 205, 903
BstPI GGTNACC 1 cut(s) 929
BstSFI CTRYAG 1 cut(s) 518
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 3 cut(s) 571, 814, 958
BstV2I GAAGAC 1 cut(s) 867
BsuRI GGCC 5 cut(s) 5, 183, 252, 413, 581
BtgI CCRYGG 1 cut(s) 178
BtgZI GCGATG 1 cut(s) 546
BtsI GCAGTG 1 cut(s) 964
BtsIMutI CAGTG 4 cut(s) 567, 657, 937, 964
Cac8I GCNNGC 2 cut(s) 279, 457
CaiI CAGNNNCTG 1 cut(s) 659
Cfr10I RCCGGY 1 cut(s) 502
Cfr13I GGNCC 3 cut(s) 182, 250, 592
Cfr42I CCGCGG 1 cut(s) 181
CpoI CGGWCCG 1 cut(s) 592
CseI GACGC 1 cut(s) 576
Csp6I GTAC 2 cut(s) 259, 942
CspI CGGWCCG 1 cut(s) 592
CviAII CATG 2 cut(s) 791, 950
CviQI GTAC 2 cut(s) 259, 942
DdeI CTNAG 2 cut(s) 92, 807
DpnI GATC 2 cut(s) 207, 905
DpnII GATC 2 cut(s) 205, 903
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 71, 89, 684
EarI CTCTTC 3 cut(s) 71, 89, 684
EciI GGCGGA 2 cut(s) 46, 89
Eco130I CCWWGG 1 cut(s) 972
Eco47I GGWCC 1 cut(s) 592
Eco57I CTGAAG 1 cut(s) 353
Eco91I GGTNACC 1 cut(s) 929
EcoO109I RGGNCCY 1 cut(s) 250
EcoO65I GGTNACC 1 cut(s) 929
EcoT14I CCWWGG 1 cut(s) 972
ErhI CCWWGG 1 cut(s) 972
Esp3I CGTCTC 1 cut(s) 362
FaeI CATG 2 cut(s) 794, 953
FaiI YATR 7 cut(s) 140, 211, 618, 792, 844, 855, 951
FaqI GGGAC 1 cut(s) 701
FatI CATG 2 cut(s) 790, 949
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 8 cut(s) 181, 439, 465, 468, 582, 585, 828, 947
Fsp4HI GCNGC 8 cut(s) 181, 439, 465, 468, 582, 585, 828, 947
FspBI CTAG 3 cut(s) 117, 882, 921
GluI GCNGC 8 cut(s) 181, 439, 465, 468, 582, 585, 828, 947
GsaI CCCAGC 1 cut(s) 784
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 5 cut(s) 5, 183, 252, 413, 581
HapII CCGG 3 cut(s) 299, 349, 503
HgaI GACGC 1 cut(s) 576
Hin1I GRCGYC 1 cut(s) 432
Hin1II CATG 2 cut(s) 794, 953
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 3 cut(s) 373, 514, 547
HpaII CCGG 3 cut(s) 299, 349, 503
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 4 cut(s) 35, 367, 592, 908
Hpy188III TCNNGA 7 cut(s) 377, 397, 635, 722, 737, 763, 822
Hpy8I GTNNAC 1 cut(s) 430
Hpy99I CGWCG 5 cut(s) 245, 248, 434, 437, 592
HpyAV CCTTC 3 cut(s) 328, 403, 622
HpyCH4III ACNGT 4 cut(s) 571, 715, 935, 941
HpyCH4IV ACGT 2 cut(s) 240, 432
HpyCH4V TGCA 6 cut(s) 404, 520, 665, 830, 962, 969
HpyF3I CTNAG 2 cut(s) 92, 807
HpySE526I ACGT 2 cut(s) 240, 432
Hsp92I GRCGYC 1 cut(s) 432
Hsp92II CATG 2 cut(s) 794, 953
KspI CCGCGG 1 cut(s) 181
Kzo9I GATC 2 cut(s) 205, 903
LmnI GCTCC 2 cut(s) 59, 803
Lsp1109I GCAGC 3 cut(s) 571, 814, 958
LweI GCATC 1 cut(s) 949
MaeI CTAG 3 cut(s) 117, 882, 921
MaeII ACGT 2 cut(s) 240, 432
MaeIII GTNAC 4 cut(s) 565, 813, 929, 935
MalI GATC 2 cut(s) 207, 905
MboI GATC 2 cut(s) 205, 903
MboII GAAGA 7 cut(s) 58, 67, 70, 76, 79, 701, 872
MhlI GDGCHC 1 cut(s) 762
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 732, 740
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 214, 570, 855
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 344, 750
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 678, 982
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 180, 584
MspI CCGG 3 cut(s) 299, 349, 503
Mva1269I GAATGC 1 cut(s) 333
MvnI CGCG 1 cut(s) 180
NdeII GATC 2 cut(s) 205, 903
NlaIII CATG 2 cut(s) 794, 953
NmeAIII GCCGAG 1 cut(s) 579
NmuCI GTSAC 2 cut(s) 565, 935
PcsI WCGNNNNNNNCGW 2 cut(s) 240, 468
PctI GAATGC 1 cut(s) 333
PdmI GAANNNNTTC 2 cut(s) 344, 750
PfeI GAWTC 3 cut(s) 373, 514, 547
PflMI CCANNNNNTGG 2 cut(s) 12, 622
PkrI GCNGC 8 cut(s) 182, 440, 466, 469, 583, 586, 829, 948
PspEI GGTNACC 1 cut(s) 929
PspFI CCCAGC 1 cut(s) 780
PspPI GGNCC 3 cut(s) 182, 250, 592
PstI CTGCAG 1 cut(s) 522
PstNI CAGNNNCTG 1 cut(s) 659
RsaI GTAC 2 cut(s) 260, 943
RsaNI GTAC 2 cut(s) 259, 942
Rsr2I CGGWCCG 1 cut(s) 592
RsrII CGGWCCG 1 cut(s) 592
SacII CCGCGG 1 cut(s) 181
SalI GTCGAC 1 cut(s) 428
SaqAI TTAA 2 cut(s) 678, 982
SatI GCNGC 8 cut(s) 181, 439, 465, 468, 582, 585, 828, 947
Sau3AI GATC 2 cut(s) 205, 903
Sau96I GGNCC 3 cut(s) 182, 250, 592
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 1 cut(s) 949
SfcI CTRYAG 1 cut(s) 518
Sfr303I CCGCGG 1 cut(s) 181
SgrBI CCGCGG 1 cut(s) 181
SinI GGWCC 1 cut(s) 592
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 2 cut(s) 732, 740
SsiI CCGC 9 cut(s) 57, 100, 178, 180, 438, 465, 468, 582, 595
SspI AATATT 1 cut(s) 607
SspMI CTAG 3 cut(s) 117, 882, 921
StyI CCWWGG 1 cut(s) 972
TaaI ACNGT 4 cut(s) 571, 715, 935, 941
TaiI ACGT 2 cut(s) 243, 435
TaqI TCGA 3 cut(s) 246, 429, 498
TasI AATT 2 cut(s) 732, 740
TauI GCSGC 5 cut(s) 183, 441, 467, 470, 584
TfiI GAWTC 3 cut(s) 373, 514, 547
Tru1I TTAA 2 cut(s) 678, 982
Tru9I TTAA 2 cut(s) 678, 982
TscAI CASTG 4 cut(s) 574, 664, 944, 971
TseFI GTSAC 2 cut(s) 565, 935
TseI GCWGC 3 cut(s) 584, 827, 946
Tsp45I GTSAC 2 cut(s) 565, 935
TspDTI ATGAA 1 cut(s) 702
TspRI CASTG 4 cut(s) 574, 664, 944, 971
Van91I CCANNNNNTGG 2 cut(s) 12, 622
VpaK11BI GGWCC 1 cut(s) 592
XapI RAATTY 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 623
XmiI GTMKAC 1 cut(s) 429
XmnI GAANNNNTTC 2 cut(s) 344, 750
XspI CTAG 3 cut(s) 117, 882, 921
ZraI GACGTC 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.