pycom15g15480

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014250
Physical Location & Seq
Reverse (-)
10776144 .. 10777698
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g15480.2

Sequence Viewer

Length: 969 bp
ATGGCTAATGCTTGGAAAAGAGACAAACCCATGAGACCCCTTTTATTCCTCTTCTCTACAACTATCGTCCTCATCTTCGTCTTCTTCTTCCTCGCCGCCCGCCCTTCCGCCACCCTCTCCACTCCCACCGCCATCGACCCCACCATCCGAACCAATCTTCCGATCTACGGAATCTCCTCATTCAATTGCGAGGAGTGCCCGCAGTCGCACCCCGTCATCGCCAACGTCGTCGAAGGCCTCCGGTACCCCTTCATCTACTCACTCGCCGATTTGGGCAGCTTGCCGGGGAAGCCCCACAAGAACATCGTCAGATTCCTCAAGAGGAAGCCGTTCCGGAAGCCCGATATCTCGGCGACGATTCAGGGGGTTCTGGAGAAATTCAAAGGAGAAGGGAGGGACAACGGGCTGGTGGTGGACGTCGGTGCCAATGTCGGCATCGCCAGCTTCGCCGCCGCCGTCATGGGTTTTCAGGTGCTTGCTTTCGAGCCGGTTTTCGAGAATTTGCAGCGGATTTGTGATGGGATTTACTTGAATCGTGTGGGGGATTTGGTGACGGTGTTCGAGGCCGCCGCCTCCGATAGCCCTGGCAATATCACCTTCCACAAGTTGGTTGGTCGGATGGACAACAGTGCTATTTCAGCCACCGGCGCAAGGCTGGCCTTCAAGAATAACGAAGAAGTGGCAGTTCAAGTAAGGACCATCCCTCTTGATGACGTGATCCAGGAATCAGAGCCTGTTCTTCTGCTCAAAATTGATGTGCAAGGTTGGGAATATCATGTGCTGAAAGGAGCTTCCAAGTTACTGTCCAGAAAGGCAGGAGAAGCACCCTATATCATCTATGAGGAAGATGAACGGCTGCTACGAGCCAGTAATAGCAGTGCAAGAGAGATACAAGAATTCCTGAAGAGTGTGGGTTACAATCACTGCACCCAGCATGGTACAGATGCACACTGCACCAAATTGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_21

Protein Analysis

323

Amino Acids

35.51

Weight (kDa)

7.66

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26680
fragaria_vesca FvH4_1g03280 FvH4_1g03280
malus_domestica MD02G1032800.v1.1
prunus_persica Prupe.7G242700_v2.0.a1
pyrus_communis pycom15g15480
rosa_chinensis RchiOBHm_Chr2g0088431
rosa_laevigata RLG00000015954 RLG00000015958
rosa_multiflora Rmu_sc0004733.1_g000021
rosa_roxburghii Rroxscaffold_2G00152670
rosa_rugosa Rorug01G0484000 Rorug01G0484100
rosa_samantha Rh2BG038000 Rh2CG039100 Rh2DG038700
rosa_wichuraiana Rw2G003070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 420
Acc65I GGTACC 1 cut(s) 243
AccB1I GGYRCC 2 cut(s) 243, 422
AccB7I CCANNNNNTGG 1 cut(s) 607
AccIII TCCGGA 1 cut(s) 333
AclWI GGATC 1 cut(s) 712
AcsI RAATTY 3 cut(s) 377, 499, 896
AcuI CTGAAG 1 cut(s) 923
AcyI GRCGYC 1 cut(s) 417
AfaI GTAC 2 cut(s) 245, 940
AfiI CCNNNNNNNGG 3 cut(s) 167, 607, 651
AgsI TTSAA 5 cut(s) 184, 382, 532, 664, 689
AjiI CACGTC 1 cut(s) 715
AjnI CCWGG 2 cut(s) 583, 720
AloI GAACNNNNNNTCC 2 cut(s) 142, 174
AluBI AGCT 3 cut(s) 279, 444, 791
AluI AGCT 3 cut(s) 279, 444, 791
Alw26I GTCTC 2 cut(s) 15, 28
AlwI GGATC 1 cut(s) 712
AlwNI CAGNNNCTG 1 cut(s) 734
Aor13HI TCCGGA 1 cut(s) 333
AoxI GGCC 3 cut(s) 235, 564, 657
ApeKI GCWGC 3 cut(s) 276, 505, 856
ApoI RAATTY 3 cut(s) 377, 499, 896
Asp700I GAANNNNTTC 1 cut(s) 329
Asp718I GGTACC 1 cut(s) 243
AspLEI GCGC 1 cut(s) 650
AspS9I GGNCC 1 cut(s) 696
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 2 cut(s) 562, 586
AvaII GGWCC 1 cut(s) 696
BaeGI GKGCMC 1 cut(s) 200
BanI GGYRCC 2 cut(s) 243, 422
BbsI GAAGAC 1 cut(s) 73
BbvI GCAGC 3 cut(s) 288, 517, 843
BccI CCATC 5 cut(s) 140, 152, 512, 613, 707
BceAI ACGGC 3 cut(s) 313, 440, 869
BciT130I CCWGG 2 cut(s) 585, 722
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 2 cut(s) 15, 28
BisI GCNGC 8 cut(s) 96, 277, 450, 453, 506, 567, 570, 857
BlsI GCNGC 8 cut(s) 97, 278, 451, 454, 507, 568, 571, 858
Bme1390I CCNGG 3 cut(s) 285, 585, 722
Bme18I GGWCC 1 cut(s) 696
BmgBI CACGTC 1 cut(s) 715
BmgT120I GGNCC 1 cut(s) 696
BmiI GGNNCC 2 cut(s) 245, 424
BmrFI CCNGG 3 cut(s) 285, 585, 722
BmsI GCATC 2 cut(s) 444, 934
BpiI GAAGAC 1 cut(s) 73
BpmI CTGGAG 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 285
BsaHI GRCGYC 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 28
BsaJI CCNNGG 2 cut(s) 284, 583
BsaWI WCCGGW 2 cut(s) 240, 333
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 607, 651
Bse118I RCCGGY 2 cut(s) 487, 644
Bse1I ACTGG 1 cut(s) 867
BseAI TCCGGA 1 cut(s) 333
BseBI CCWGG 2 cut(s) 585, 722
BseDI CCNNGG 2 cut(s) 284, 583
BseGI GGATG 3 cut(s) 144, 624, 699
BseLI CCNNNNNNNGG 3 cut(s) 167, 607, 651
BseNI ACTGG 1 cut(s) 867
BseRI GAGGAG 2 cut(s) 166, 206
BseSI GKGCMC 1 cut(s) 200
BseXI GCAGC 3 cut(s) 288, 517, 843
BseYI CCCAGC 1 cut(s) 930
BsgI GTGCAG 2 cut(s) 910, 937
BshFI GGCC 3 cut(s) 237, 566, 659
BshNI GGYRCC 2 cut(s) 243, 422
BsiSI CCGG 5 cut(s) 241, 284, 334, 488, 645
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 3 cut(s) 167, 607, 651
BsmAI GTCTC 2 cut(s) 15, 28
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 3 cut(s) 237, 566, 659
Bso31I GGTCTC 1 cut(s) 28
Bsp1286I GDGCHC 1 cut(s) 200
Bsp13I TCCGGA 1 cut(s) 333
Bsp143I GATC 2 cut(s) 162, 717
BspANI GGCC 3 cut(s) 237, 566, 659
BspEI TCCGGA 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 245, 424
BspPI GGATC 1 cut(s) 712
BspT107I GGYRCC 2 cut(s) 243, 422
BspTNI GGTCTC 1 cut(s) 28
BsrFI RCCGGY 2 cut(s) 487, 644
BsrI ACTGG 1 cut(s) 867
BssAI RCCGGY 2 cut(s) 487, 644
BssECI CCNNGG 2 cut(s) 284, 583
BssMI GATC 2 cut(s) 162, 717
BssNI GRCGYC 1 cut(s) 417
Bst2UI CCWGG 2 cut(s) 585, 722
Bst4CI ACNGT 3 cut(s) 556, 629, 804
Bst6I CTCTTC 2 cut(s) 56, 899
BstACI GRCGYC 1 cut(s) 417
BstC8I GCNNGC 6 cut(s) 100, 200, 281, 442, 477, 657
BstF5I GGATG 3 cut(s) 144, 624, 699
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 2 cut(s) 165, 720
BstMAI GTCTC 2 cut(s) 15, 28
BstMBI GATC 2 cut(s) 162, 717
BstMWI GCNNNNNNNGC 8 cut(s) 195, 289, 441, 446, 638, 647, 656, 821
BstNI CCWGG 2 cut(s) 585, 722
BstSCI CCNGG 3 cut(s) 283, 583, 720
BstSLI GKGCMC 1 cut(s) 200
BstV1I GCAGC 3 cut(s) 288, 517, 843
BstV2I GAAGAC 1 cut(s) 73
BsuRI GGCC 3 cut(s) 237, 566, 659
BtgZI GCGATG 2 cut(s) 202, 421
BtrI CACGTC 1 cut(s) 715
BtsCI GGATG 3 cut(s) 144, 624, 699
BtsI GCAGTG 3 cut(s) 883, 922, 949
BtsIMutI CAGTG 4 cut(s) 634, 883, 922, 949
Cac8I GCNNGC 6 cut(s) 100, 200, 281, 442, 477, 657
CaiI CAGNNNCTG 1 cut(s) 734
CfoI GCGC 1 cut(s) 650
Cfr10I RCCGGY 2 cut(s) 487, 644
Cfr13I GGNCC 1 cut(s) 696
Csp6I GTAC 2 cut(s) 244, 939
CviAII CATG 4 cut(s) 31, 460, 776, 935
CviQI GTAC 2 cut(s) 244, 939
DpnI GATC 2 cut(s) 164, 719
DpnII GATC 2 cut(s) 162, 717
Eam1104I CTCTTC 2 cut(s) 56, 899
EarI CTCTTC 2 cut(s) 56, 899
EciI GGCGGA 1 cut(s) 97
Eco147I AGGCCT 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 28
Eco32I GATATC 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 696
Eco57I CTGAAG 1 cut(s) 923
EcoRI GAATTC 1 cut(s) 896
EcoRII CCWGG 2 cut(s) 583, 720
EcoRV GATATC 1 cut(s) 346
FaeI CATG 4 cut(s) 34, 463, 779, 938
FaiI YATR 6 cut(s) 32, 461, 777, 831, 840, 936
FaqI GGGAC 1 cut(s) 410
FatI CATG 4 cut(s) 30, 459, 775, 934
FauI CCCGC 2 cut(s) 107, 207
Fnu4HI GCNGC 8 cut(s) 96, 277, 450, 453, 506, 567, 570, 857
FokI GGATG 3 cut(s) 131, 631, 686
Fsp4HI GCNGC 8 cut(s) 96, 277, 450, 453, 506, 567, 570, 857
GlaI GCGC 1 cut(s) 649
GluI GCNGC 8 cut(s) 96, 277, 450, 453, 506, 567, 570, 857
GsaI CCCAGC 1 cut(s) 934
GsuI CTGGAG 1 cut(s) 392
HaeIII GGCC 3 cut(s) 237, 566, 659
HapII CCGG 5 cut(s) 241, 284, 334, 488, 645
HhaI GCGC 1 cut(s) 650
Hin1I GRCGYC 1 cut(s) 417
Hin1II CATG 4 cut(s) 34, 463, 779, 938
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HinfI GANTC 5 cut(s) 171, 312, 358, 532, 725
HpaII CCGG 5 cut(s) 241, 284, 334, 488, 645
HphI GGTGA 2 cut(s) 562, 586
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 6 cut(s) 149, 162, 311, 577, 618, 730
Hpy188III TCNNGA 8 cut(s) 319, 334, 371, 496, 664, 707, 807, 901
Hpy8I GTNNAC 1 cut(s) 415
Hpy99I CGWCG 4 cut(s) 230, 233, 358, 422
HpyAV CCTTC 6 cut(s) 114, 227, 259, 383, 607, 670
HpyCH4III ACNGT 3 cut(s) 556, 629, 804
HpyCH4IV ACGT 3 cut(s) 225, 417, 714
HpyCH4V TGCA 6 cut(s) 505, 760, 881, 927, 947, 954
HpyF10VI GCNNNNNNNGC 8 cut(s) 195, 289, 441, 446, 638, 647, 656, 821
HpySE526I ACGT 3 cut(s) 225, 417, 714
Hsp92I GRCGYC 1 cut(s) 417
Hsp92II CATG 4 cut(s) 34, 463, 779, 938
HspAI GCGC 1 cut(s) 648
Kpn2I TCCGGA 1 cut(s) 333
KpnI GGTACC 1 cut(s) 247
Kzo9I GATC 2 cut(s) 162, 717
LmnI GCTCC 1 cut(s) 788
Lsp1109I GCAGC 3 cut(s) 288, 517, 843
LweI GCATC 2 cut(s) 444, 934
MaeII ACGT 3 cut(s) 225, 417, 714
MaeIII GTNAC 3 cut(s) 550, 798, 914
MalI GATC 2 cut(s) 164, 719
MboI GATC 2 cut(s) 162, 717
MfeI CAATTG 1 cut(s) 184
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 6 cut(s) 184, 377, 499, 750, 896, 959
MmeI TCCRAC 1 cut(s) 596
MroI TCCGGA 1 cut(s) 333
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 1 cut(s) 967
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 5 cut(s) 241, 284, 334, 488, 645
MspR9I CCNGG 3 cut(s) 285, 585, 722
MunI CAATTG 1 cut(s) 184
MvaI CCWGG 2 cut(s) 585, 722
MwoI GCNNNNNNNGC 8 cut(s) 195, 289, 441, 446, 638, 647, 656, 821
NciI CCSGG 1 cut(s) 285
NdeII GATC 2 cut(s) 162, 717
NlaIII CATG 4 cut(s) 34, 463, 779, 938
NlaIV GGNNCC 2 cut(s) 245, 424
NmeAIII GCCGAG 1 cut(s) 329
NmuCI GTSAC 1 cut(s) 550
PceI AGGCCT 1 cut(s) 237
PcsI WCGNNNNNNNCGW 3 cut(s) 225, 453, 859
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 5 cut(s) 171, 312, 358, 532, 725
PflMI CCANNNNNTGG 1 cut(s) 607
PfoI TCCNGGA 1 cut(s) 720
PkrI GCNGC 8 cut(s) 97, 278, 451, 454, 507, 568, 571, 858
Psp6I CCWGG 2 cut(s) 583, 720
PspFI CCCAGC 1 cut(s) 930
PspGI CCWGG 2 cut(s) 583, 720
PspN4I GGNNCC 2 cut(s) 245, 424
PspPI GGNCC 1 cut(s) 696
PsrI GAACNNNNNNTAC 2 cut(s) 843, 875
PstNI CAGNNNCTG 1 cut(s) 734
RsaI GTAC 2 cut(s) 245, 940
RsaNI GTAC 2 cut(s) 244, 939
SaqAI TTAA 1 cut(s) 967
SatI GCNGC 8 cut(s) 96, 277, 450, 453, 506, 567, 570, 857
Sau3AI GATC 2 cut(s) 162, 717
Sau96I GGNCC 1 cut(s) 696
ScrFI CCNGG 3 cut(s) 285, 585, 722
SduI GDGCHC 1 cut(s) 200
SetI ASST 9 cut(s) 228, 281, 420, 446, 474, 599, 717, 766, 793
SfaNI GCATC 2 cut(s) 444, 934
SgrAI CRCCGGYG 1 cut(s) 644
SinI GGWCC 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 6 cut(s) 184, 377, 499, 750, 896, 959
SseBI AGGCCT 1 cut(s) 237
StuI AGGCCT 1 cut(s) 237
StyD4I CCNGG 3 cut(s) 283, 583, 720
TaaI ACNGT 3 cut(s) 556, 629, 804
TaiI ACGT 3 cut(s) 228, 420, 717
TaqI TCGA 5 cut(s) 135, 231, 483, 495, 561
TasI AATT 6 cut(s) 184, 377, 499, 750, 896, 959
TauI GCSGC 5 cut(s) 98, 452, 455, 569, 572
TfiI GAWTC 5 cut(s) 171, 312, 358, 532, 725
Tru1I TTAA 1 cut(s) 967
Tru9I TTAA 1 cut(s) 967
TscAI CASTG 4 cut(s) 634, 883, 929, 956
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 3 cut(s) 276, 505, 856
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 2 cut(s) 241, 864
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 4 cut(s) 634, 883, 929, 956
Van91I CCANNNNNTGG 1 cut(s) 607
VpaK11BI GGWCC 1 cut(s) 696
XapI RAATTY 3 cut(s) 377, 499, 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 608
XmnI GAANNNNTTC 1 cut(s) 329
ZraI GACGTC 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.