MD02G1049300.v1.1

Mitochondrial domain of unknown function (DUF1713)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008619
Physical Location & Seq
Reverse (-)
3866850 .. 3869105
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1049300.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGTGAGAAATGTTTTGCAATATCCCCTGAATTCAATTCACCATCAGCTCTTTCTTGTTCCCTGCGGTCGCCATTCTCCTCTTCGAAACGAAACCCTTTTCGGCATAGCCAAAAACGCCATAGCAAATCAATCTTTTCAAATCCTCCAAACCGTGCGTTGCTCTGCGACGGAGCAACCTGTGATTCATCAATTTCTCCTCCTCTTGCCGCCCCAGATGGCGAGTTTTGTCCACAAACTTGCTAAAAACGCACCGGCGTTAAGAACAGCGGCGTCCCTCAAATCCCATCTCTTCCCCAAACCCACAGCCTCCCTTATTTCTCACCATAGCCATCTGGGCGCTCCCAAACCCGAATTCTCAGAAGACCCGGTTGCCCAATTAGGTCCCGCAAGCTTCCACAATGCTGAATCTCCCAATCCCTTCCCTATTTTCCCCAATTTCTGCTTAAATCCGATTTCTCCAGCTGGGTTCGATCGGTTGGGTGTTTTAGAGGCGGTCGAGGTGGAGTCCGATGATGTGAGGACGATTTGGGCTGACAGTGTGAAGAAGAAGAGGAAGAAGAAGATGAACAAGCATAAGTTAAAGAAGCTAAGGAAGAGTCTTTGCCGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1713

Protein Analysis

205

Amino Acids

22.8

Weight (kDa)

10.02

Isoelectric Point (pI)

54.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COX24_C PF08213 178 - 199 1.4e-07 Mitochondrial mRNA-processing protein COX24, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 66, 209, 269, 387, 494, 607
AcsI RAATTY 2 cut(s) 31, 353
AcyI GRCGYC 1 cut(s) 272
AgsI TTSAA 2 cut(s) 36, 140
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AluBI AGCT 4 cut(s) 49, 393, 464, 589
AluI AGCT 4 cut(s) 49, 393, 464, 589
ApoI RAATTY 2 cut(s) 31, 353
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 383
AsuC2I CCSGG 1 cut(s) 368
AsuHPI GGTGA 3 cut(s) 16, 32, 314
AsuII TTCGAA 1 cut(s) 85
AvaII GGWCC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 369
BccI CCATC 4 cut(s) 51, 211, 294, 339
BcnI CCSGG 1 cut(s) 368
BfoI RGCGCY 1 cut(s) 342
BglI GCCNNNNNGGC 1 cut(s) 336
BisI GCNGC 3 cut(s) 209, 270, 607
BlsI GCNGC 3 cut(s) 210, 271, 608
Bme1390I CCNGG 1 cut(s) 368
Bme18I GGWCC 1 cut(s) 383
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 444
Bpu10I CCTNAGC 1 cut(s) 590
Bpu14I TTCGAA 1 cut(s) 85
BpuMI CCSGG 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 272
Bse118I RCCGGY 1 cut(s) 253
BseMII CTCAG 1 cut(s) 372
BseRI GAGGAG 3 cut(s) 69, 188, 191
BseYI CCCAGC 1 cut(s) 464
Bsh1285I CGRYCG 3 cut(s) 70, 475, 498
BsiEI CGRYCG 3 cut(s) 70, 475, 498
BsiSI CCGG 2 cut(s) 254, 368
BslFI GGGAC 2 cut(s) 259, 369
BsmFI GGGAC 2 cut(s) 259, 369
Bsp119I TTCGAA 1 cut(s) 85
Bsp143I GATC 1 cut(s) 472
BspACI CCGC 6 cut(s) 66, 209, 269, 387, 494, 607
BspCNI CTCAG 1 cut(s) 371
BspLI GGNNCC 1 cut(s) 385
BspT104I TTCGAA 1 cut(s) 85
BsrFI RCCGGY 1 cut(s) 253
BssAI RCCGGY 1 cut(s) 253
BssMI GATC 1 cut(s) 472
BssNI GRCGYC 1 cut(s) 272
Bst4CI ACNGT 2 cut(s) 154, 539
Bst6I CTCTTC 4 cut(s) 87, 296, 545, 590
BstACI GRCGYC 1 cut(s) 272
BstBI TTCGAA 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 391
BstDEI CTNAG 2 cut(s) 358, 590
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 1 cut(s) 475
BstMBI GATC 1 cut(s) 472
BstMCI CGRYCG 3 cut(s) 70, 475, 498
BstMWI GCNNNNNNNGC 3 cut(s) 116, 248, 336
BstSCI CCNGG 1 cut(s) 366
BstV2I GAAGAC 1 cut(s) 369
BtsIMutI CAGTG 1 cut(s) 544
Cac8I GCNNGC 1 cut(s) 391
CfoI GCGC 1 cut(s) 341
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 383
CseI GACGC 1 cut(s) 261
CviJI RGCY 8 cut(s) 49, 110, 308, 330, 393, 464, 533, 589
CviKI_1 RGCY 8 cut(s) 49, 110, 308, 330, 393, 464, 533, 589
DdeI CTNAG 2 cut(s) 358, 590
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
Eam1104I CTCTTC 4 cut(s) 87, 296, 545, 590
EarI CTCTTC 4 cut(s) 87, 296, 545, 590
Eco47I GGWCC 1 cut(s) 383
EcoO109I RGGNCCY 1 cut(s) 383
EcoRI GAATTC 2 cut(s) 31, 353
FaiI YATR 4 cut(s) 107, 122, 327, 576
FaqI GGGAC 2 cut(s) 259, 369
FauI CCCGC 1 cut(s) 394
Fnu4HI GCNGC 3 cut(s) 209, 270, 607
Fsp4HI GCNGC 3 cut(s) 209, 270, 607
GlaI GCGC 1 cut(s) 340
GluI GCNGC 3 cut(s) 209, 270, 607
GsaI CCCAGC 1 cut(s) 468
GsuI CTGGAG 1 cut(s) 444
HaeII RGCGCY 1 cut(s) 342
HapII CCGG 2 cut(s) 254, 368
HgaI GACGC 1 cut(s) 261
HhaI GCGC 1 cut(s) 341
Hin1I GRCGYC 1 cut(s) 272
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HindIII AAGCTT 1 cut(s) 391
HinfI GANTC 4 cut(s) 184, 407, 506, 598
HpaII CCGG 2 cut(s) 254, 368
HphI GGTGA 3 cut(s) 16, 32, 314
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 3 cut(s) 361, 453, 511
Hpy8I GTNNAC 1 cut(s) 232
Hpy99I CGWCG 1 cut(s) 172
HpyAV CCTTC 1 cut(s) 430
HpyCH4III ACNGT 2 cut(s) 154, 539
HpyCH4V TGCA 1 cut(s) 19
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 248, 336
HpyF3I CTNAG 2 cut(s) 358, 590
Hsp92I GRCGYC 1 cut(s) 272
HspAI GCGC 1 cut(s) 339
Kzo9I GATC 1 cut(s) 472
LmnI GCTCC 2 cut(s) 172, 346
LpnPI CCDG 9 cut(s) 41, 76, 192, 227, 267, 320, 381, 450, 474
MalI GATC 1 cut(s) 474
MboI GATC 1 cut(s) 472
MluCI AATT 7 cut(s) 31, 36, 191, 353, 377, 436, 610
MlyI GAGTC 2 cut(s) 515, 607
MseI TTAA 3 cut(s) 260, 446, 581
MspA1I CMGCKG 2 cut(s) 269, 464
MspI CCGG 2 cut(s) 254, 368
MspR9I CCNGG 1 cut(s) 368
MwoI GCNNNNNNNGC 3 cut(s) 116, 248, 336
NciI CCSGG 1 cut(s) 368
NdeII GATC 1 cut(s) 472
NlaIV GGNNCC 1 cut(s) 385
NspV TTCGAA 1 cut(s) 85
PfeI GAWTC 2 cut(s) 184, 407
PkrI GCNGC 3 cut(s) 210, 271, 608
Ple19I CGATCG 1 cut(s) 475
PleI GAGTC 2 cut(s) 514, 606
PpsI GAGTC 2 cut(s) 514, 606
PpuMI RGGWCCY 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 383
PspFI CCCAGC 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 385
PspPI GGNCC 1 cut(s) 383
PspPPI RGGWCCY 1 cut(s) 383
PvuI CGATCG 1 cut(s) 475
PvuII CAGCTG 1 cut(s) 464
SaqAI TTAA 3 cut(s) 260, 446, 581
SatI GCNGC 3 cut(s) 209, 270, 607
Sau3AI GATC 1 cut(s) 472
Sau96I GGNCC 1 cut(s) 383
SchI GAGTC 2 cut(s) 515, 607
ScrFI CCNGG 1 cut(s) 368
SetI ASST 7 cut(s) 51, 181, 385, 395, 466, 504, 591
SfuI TTCGAA 1 cut(s) 85
SgrAI CRCCGGYG 1 cut(s) 253
SinI GGWCC 1 cut(s) 383
Sse9I AATT 7 cut(s) 31, 36, 191, 353, 377, 436, 610
SsiI CCGC 6 cut(s) 66, 209, 269, 387, 494, 607
StyD4I CCNGG 1 cut(s) 366
TaaI ACNGT 2 cut(s) 154, 539
TaqI TCGA 3 cut(s) 85, 471, 498
TasI AATT 7 cut(s) 31, 36, 191, 353, 377, 436, 610
TauI GCSGC 3 cut(s) 211, 272, 609
TfiI GAWTC 2 cut(s) 184, 407
Tru1I TTAA 3 cut(s) 260, 446, 581
Tru9I TTAA 3 cut(s) 260, 446, 581
TscAI CASTG 1 cut(s) 544
TspDTI ATGAA 2 cut(s) 176, 581
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 383
XapI RAATTY 2 cut(s) 31, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.