Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0006040

Physical Location & Seq

Genomic Coordinates Reverse (-)

3871079 .. 3872473

Gene Structure 1395 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1049600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 804 bp

ATGCACTTTCGTGTACTAGCTAAAGCGTTACGTATGTCTGGTGGAGATCATATACACGTTGGTACCGTAGTAGGTAAACTTAAGGGGGAAAGAGAGATTACTTTAGGCTTTGTTGATTTATTACGTGATGATTTTGTTGAAAAAGATCGAAGTCGCGGTATTTATTTCACTCAAGATTGGGTCTCTCTGCCAGGTGTTTTGCTTGTGGCTTCAGGGGGTATTCACGTTTGGCATATGCCTGCTCTGACCAAGATCCTTGCAGATGATTCTGTACTACAATTTGGTGGCGAAACTTTAGGACACCCTTGGGGAAATGCACCTGGTGCCGTAGCTAATCGAGTAGCTCTAGAAGCATGTGTACAAGCTCGTAATGAGGGACGTGATCTTGCTCGTGAGGGTAATGAAATTATTCGTGAGGCTAGTAAATGGAGTCCTGAACTAGCTGCTGCTTGTGAAATATGGAAGGAGATCAATTCGAATTCGAAGCAATGGATACTTTGTAGTCCAGGGCAAGAAAGTTCTATGGAAAAATGGTGGTTCGATTCGATGTTGTTTAAAGGGGAGTTAGAATACATGTGTAGGCTAAGTAAATCAATGGACAGTCTTGGTCCTTTTGAAAATACCAGTGTAAGTGAAGATCCAATTTTAAATGATATGGATAAAGACATTTTTAATTTTAGCGACAAGTCTAATTACAGTAATGTTGATCATTTAGTCGGTGACAGATACATTCGGAATTTCATATCTGATGACACTTTTTTCGTTAGGGATAGTAATAGGGACAATTATTCCATATATTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00061 ko00620 ko00630 ko00640 ko00710 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00630 map00640 map00710 map00720 map01100 map01110 map01120 map01130 map01200 map01212

Pfam Domains

Protein Families

RuBisCO_large RuBisCO_large_N

Protein Analysis

268

Amino Acids

30.22

Weight (kDa)

5.13

Isoelectric Point (pI)

38.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RuBisCO_large	PF00016	1 - 154	9.7e-52	Ribulose bisphosphate carboxylase large chain, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006040)

Species	Orthologous Gene IDs
arabidopsis_thaliana	ATCG00490
malus_domestica	MD02G1049600.v1.1 MD11G1294900.v1.1 MD11G1295000.v1.1
prunus_persica	Prupe.7G029600_v2.0.a1
pyrus_communis	pycom08g13270 pycom12397g00250
rosa_chinensis	RchiOBHm_Chr5g0043011 RchiOBHm_CPg0502111
rosa_laevigata	RLG00000000069 RLG00000000106 RLG00000000163 RLG00000000221 RLG00000000257 RLG00000000267 RLG00000000290 RLG00000000329 RLG00000000350 RLG00000037105
rosa_roxburghii	Rroxscaffold_2G00087120 Rroxscaffold_3G00220720
rosa_rugosa	RorugMtG0002400.1
rosa_samantha	Rh2AG282300 Rh2BG293600 Rh2DG288500 Rh5BG295000 Rh5DG304800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	62
AccB1I	GGYRCC	2 cut(s)	62, 323
AccII	CGCG	1 cut(s)	156
AciI	CCGC	1 cut(s)	156
AclWI	GGATC	2 cut(s)	247, 632
AcsI	RAATTY	2 cut(s)	478, 736
AcuI	CTGAAG	1 cut(s)	195
AdeI	CACNNNGTG	1 cut(s)	323
AfaI	GTAC	4 cut(s)	15, 64, 273, 360
AflII	CTTAAG	1 cut(s)	80
AflIII	ACRYGT	2 cut(s)	55, 573
AgsI	TTSAA	2 cut(s)	140, 617
AjiI	CACGTC	1 cut(s)	380
AjnI	CCWGG	3 cut(s)	190, 319, 505
AleI	CACNNNNGTG	1 cut(s)	9
AluBI	AGCT	5 cut(s)	20, 332, 344, 365, 443
AluI	AGCT	5 cut(s)	20, 332, 344, 365, 443
Alw26I	GTCTC	1 cut(s)	187
AlwI	GGATC	2 cut(s)	247, 632
ApeKI	GCWGC	2 cut(s)	443, 446
ApoI	RAATTY	2 cut(s)	478, 736
ArsI	GACNNNNNNTTYG	2 cut(s)	743, 775
Asp700I	GAANNNNTTC	1 cut(s)	408
Asp718I	GGTACC	1 cut(s)	62
AspS9I	GGNCC	1 cut(s)	608
AsuHPI	GGTGA	1 cut(s)	731
AsuII	TTCGAA	2 cut(s)	476, 482
AvaII	GGWCC	1 cut(s)	608
BaeI	ACNNNNGTAYC	4 cut(s)	46, 54, 79, 87
BanI	GGYRCC	2 cut(s)	62, 323
BarI	GAAGNNNNNNTAC	2 cut(s)	342, 374
BauI	CACGAG	1 cut(s)	390
BbvI	GCAGC	2 cut(s)	430, 433
BceAI	ACGGC	1 cut(s)	311
BciT130I	CCWGG	3 cut(s)	192, 321, 507
BciVI	GTATCC	1 cut(s)	486
BclI	TGATCA	1 cut(s)	706
BcoDI	GTCTC	1 cut(s)	187
BfaI	CTAG	4 cut(s)	17, 347, 420, 440
BfrI	CTTAAG	1 cut(s)	80
BfuI	GTATCC	1 cut(s)	486
BisI	GCNGC	2 cut(s)	444, 447
BlsI	GCNGC	2 cut(s)	445, 448
Bme1390I	CCNGG	3 cut(s)	192, 321, 507
Bme18I	GGWCC	1 cut(s)	608
BmgBI	CACGTC	1 cut(s)	380
BmgT120I	GGNCC	1 cut(s)	608
BmiI	GGNNCC	2 cut(s)	64, 325
BmrFI	CCNGG	3 cut(s)	192, 321, 507
Bpu14I	TTCGAA	2 cut(s)	476, 482
BpuEI	CTTGAG	1 cut(s)	156
BsaAI	YACGTR	2 cut(s)	32, 125
BsaI	GGTCTC	1 cut(s)	187
BsaJI	CCNNGG	2 cut(s)	305, 506
Bse1I	ACTGG	1 cut(s)	624
Bse3DI	GCAATG	1 cut(s)	494
BseBI	CCWGG	3 cut(s)	192, 321, 507
BseDI	CCNNGG	2 cut(s)	305, 506
BseMI	GCAATG	1 cut(s)	494
BseNI	ACTGG	1 cut(s)	624
BseXI	GCAGC	2 cut(s)	430, 433
Bsh1236I	CGCG	1 cut(s)	156
BshNI	GGYRCC	2 cut(s)	62, 323
BslFI	GGGAC	2 cut(s)	390, 794
BsmAI	GTCTC	1 cut(s)	187
BsmFI	GGGAC	2 cut(s)	390, 794
Bso31I	GGTCTC	1 cut(s)	187
Bsp119I	TTCGAA	2 cut(s)	476, 482
Bsp1407I	TGTACA	1 cut(s)	358
Bsp143I	GATC	7 cut(s)	46, 145, 252, 382, 468, 637, 706
BspACI	CCGC	1 cut(s)	156
BspFNI	CGCG	1 cut(s)	156
BspLI	GGNNCC	2 cut(s)	64, 325
BspPI	GGATC	2 cut(s)	247, 632
BspT104I	TTCGAA	2 cut(s)	476, 482
BspT107I	GGYRCC	2 cut(s)	62, 323
BspTI	CTTAAG	1 cut(s)	80
BspTNI	GGTCTC	1 cut(s)	187
BsrDI	GCAATG	1 cut(s)	494
BsrGI	TGTACA	1 cut(s)	358
BsrI	ACTGG	1 cut(s)	624
BssECI	CCNNGG	2 cut(s)	305, 506
BssMI	GATC	7 cut(s)	46, 145, 252, 382, 468, 637, 706
BssSI	CACGAG	1 cut(s)	390
BssT1I	CCWWGG	1 cut(s)	305
Bst2BI	CACGAG	1 cut(s)	390
Bst2UI	CCWGG	3 cut(s)	192, 321, 507
Bst4CI	ACNGT	3 cut(s)	67, 602, 698
BstAFI	CTTAAG	1 cut(s)	80
BstAPI	GCANNNNNTGC	1 cut(s)	323
BstAUI	TGTACA	1 cut(s)	358
BstBAI	YACGTR	2 cut(s)	32, 125
BstBI	TTCGAA	2 cut(s)	476, 482
BstC8I	GCNNGC	1 cut(s)	240
BstDEI	CTNAG	1 cut(s)	584
BstFNI	CGCG	1 cut(s)	156
BstKTI	GATC	7 cut(s)	49, 148, 255, 385, 471, 640, 709
BstMAI	GTCTC	1 cut(s)	187
BstMBI	GATC	7 cut(s)	46, 145, 252, 382, 468, 637, 706
BstMWI	GCNNNNNNNGC	2 cut(s)	323, 350
BstNI	CCWGG	3 cut(s)	192, 321, 507
BstNSI	RCATGY	2 cut(s)	357, 577
BstSCI	CCNGG	3 cut(s)	190, 319, 505
BstSNI	TACGTA	1 cut(s)	32
BstUI	CGCG	1 cut(s)	156
BstV1I	GCAGC	2 cut(s)	430, 433
BstX2I	RGATCY	2 cut(s)	252, 637
BstYI	RGATCY	2 cut(s)	252, 637
BsuI	GTATCC	1 cut(s)	486
BtrI	CACGTC	1 cut(s)	380
BtsIMutI	CAGTG	1 cut(s)	631
Cac8I	GCNNGC	1 cut(s)	240
Cfr13I	GGNCC	1 cut(s)	608
CsiI	ACCWGGT	1 cut(s)	319
Csp6I	GTAC	4 cut(s)	14, 63, 272, 359
CviAII	CATG	2 cut(s)	354, 574
CviJI	RGCY	9 cut(s)	20, 108, 209, 332, 344, 365, 419, 443, 583
CviKI_1	RGCY	9 cut(s)	20, 108, 209, 332, 344, 365, 419, 443, 583
CviQI	GTAC	4 cut(s)	14, 63, 272, 359
DdeI	CTNAG	1 cut(s)	584
DpnI	GATC	7 cut(s)	48, 147, 254, 384, 470, 639, 708
DpnII	GATC	7 cut(s)	46, 145, 252, 382, 468, 637, 706
DraI	TTTAAA	2 cut(s)	556, 648
DraIII	CACNNNGTG	1 cut(s)	323
Eco105I	TACGTA	1 cut(s)	32
Eco130I	CCWWGG	1 cut(s)	305
Eco31I	GGTCTC	1 cut(s)	187
Eco47I	GGWCC	1 cut(s)	608
Eco57I	CTGAAG	1 cut(s)	195
EcoRI	GAATTC	1 cut(s)	478
EcoRII	CCWGG	3 cut(s)	190, 319, 505
EcoT14I	CCWWGG	1 cut(s)	305
ErhI	CCWWGG	1 cut(s)	305
FaeI	CATG	2 cut(s)	357, 577
FaqI	GGGAC	2 cut(s)	390, 794
FatI	CATG	2 cut(s)	353, 573
FauNDI	CATATG	1 cut(s)	234
FbaI	TGATCA	1 cut(s)	706
Fnu4HI	GCNGC	2 cut(s)	444, 447
Fsp4HI	GCNGC	2 cut(s)	444, 447
FspBI	CTAG	4 cut(s)	17, 347, 420, 440
GluI	GCNGC	2 cut(s)	444, 447
Hin1II	CATG	2 cut(s)	357, 577
HinfI	GANTC	3 cut(s)	266, 430, 542
HphI	GGTGA	1 cut(s)	731
Hpy166II	GTNNAC	3 cut(s)	14, 77, 359
Hpy188I	TCNGA	3 cut(s)	246, 735, 748
Hpy188III	TCNNGA	5 cut(s)	173, 347, 392, 413, 434
Hpy8I	GTNNAC	3 cut(s)	14, 77, 359
HpyAV	CCTTC	1 cut(s)	457
HpyCH4III	ACNGT	3 cut(s)	67, 602, 698
HpyCH4IV	ACGT	5 cut(s)	31, 57, 124, 225, 379
HpyCH4V	TGCA	3 cut(s)	4, 260, 317
HpyF10VI	GCNNNNNNNGC	2 cut(s)	323, 350
HpyF3I	CTNAG	1 cut(s)	584
HpySE526I	ACGT	5 cut(s)	31, 57, 124, 225, 379
Hsp92II	CATG	2 cut(s)	357, 577
KpnI	GGTACC	1 cut(s)	66
Ksp22I	TGATCA	1 cut(s)	706
Kzo9I	GATC	7 cut(s)	46, 145, 252, 382, 468, 637, 706
Lsp1109I	GCAGC	2 cut(s)	430, 433
MabI	ACCWGGT	1 cut(s)	319
MaeI	CTAG	4 cut(s)	17, 347, 420, 440
MaeII	ACGT	5 cut(s)	31, 57, 124, 225, 379
MaeIII	GTNAC	2 cut(s)	27, 719
MalI	GATC	7 cut(s)	48, 147, 254, 384, 470, 639, 708
MboI	GATC	7 cut(s)	46, 145, 252, 382, 468, 637, 706
MboII	GAAGA	1 cut(s)	647
MflI	RGATCY	2 cut(s)	252, 637
MluCI	AATT	9 cut(s)	278, 405, 472, 478, 642, 673, 691, 736, 784
MlyI	GAGTC	1 cut(s)	439
MnlI	CCTC	3 cut(s)	367, 388, 409
MroXI	GAANNNNTTC	1 cut(s)	408
MseI	TTAA	4 cut(s)	81, 555, 647, 672
MslI	CAYNNNNRTG	1 cut(s)	9
MspCI	CTTAAG	1 cut(s)	80
MspR9I	CCNGG	3 cut(s)	192, 321, 507
MvaI	CCWGG	3 cut(s)	192, 321, 507
MvnI	CGCG	1 cut(s)	156
MwoI	GCNNNNNNNGC	2 cut(s)	323, 350
NdeI	CATATG	1 cut(s)	234
NdeII	GATC	7 cut(s)	46, 145, 252, 382, 468, 637, 706
NlaIII	CATG	2 cut(s)	357, 577
NlaIV	GGNNCC	2 cut(s)	64, 325
NmuCI	GTSAC	1 cut(s)	719
NspI	RCATGY	2 cut(s)	357, 577
NspV	TTCGAA	2 cut(s)	476, 482
OliI	CACNNNNGTG	1 cut(s)	9
PciI	ACATGT	1 cut(s)	573
PcsI	WCGNNNNNNNCGW	1 cut(s)	63
PdmI	GAANNNNTTC	1 cut(s)	408
PfeI	GAWTC	2 cut(s)	266, 542
PkrI	GCNGC	2 cut(s)	445, 448
PleI	GAGTC	1 cut(s)	438
PpsI	GAGTC	1 cut(s)	438
Ppu21I	YACGTR	2 cut(s)	32, 125
PscI	ACATGT	1 cut(s)	573
Psp6I	CCWGG	3 cut(s)	190, 319, 505
PspGI	CCWGG	3 cut(s)	190, 319, 505
PspN4I	GGNNCC	2 cut(s)	64, 325
PspPI	GGNCC	1 cut(s)	608
PsuI	RGATCY	2 cut(s)	252, 637
RsaI	GTAC	4 cut(s)	15, 64, 273, 360
RsaNI	GTAC	4 cut(s)	14, 63, 272, 359
RseI	CAYNNNNRTG	1 cut(s)	9
SaqAI	TTAA	4 cut(s)	81, 555, 647, 672
SatI	GCNGC	2 cut(s)	444, 447
Sau3AI	GATC	7 cut(s)	46, 145, 252, 382, 468, 637, 706
Sau96I	GGNCC	1 cut(s)	608
SchI	GAGTC	1 cut(s)	439
ScrFI	CCNGG	3 cut(s)	192, 321, 507
SexAI	ACCWGGT	1 cut(s)	319
SfuI	TTCGAA	2 cut(s)	476, 482
SinI	GGWCC	1 cut(s)	608
SmiMI	CAYNNNNRTG	1 cut(s)	9
SmlI	CTYRAG	2 cut(s)	80, 171
SmoI	CTYRAG	2 cut(s)	80, 171
SnaBI	TACGTA	1 cut(s)	32
Sse9I	AATT	9 cut(s)	278, 405, 472, 478, 642, 673, 691, 736, 784
SsiI	CCGC	1 cut(s)	156
SspMI	CTAG	4 cut(s)	17, 347, 420, 440
StyD4I	CCNGG	3 cut(s)	190, 319, 505
StyI	CCWWGG	1 cut(s)	305
TaaI	ACNGT	3 cut(s)	67, 602, 698
TaiI	ACGT	5 cut(s)	34, 60, 127, 228, 382
TaqI	TCGA	6 cut(s)	148, 337, 476, 482, 540, 545
TasI	AATT	9 cut(s)	278, 405, 472, 478, 642, 673, 691, 736, 784
TatI	WGTACW	3 cut(s)	13, 271, 358
TfiI	GAWTC	2 cut(s)	266, 542
Tru1I	TTAA	4 cut(s)	81, 555, 647, 672
Tru9I	TTAA	4 cut(s)	81, 555, 647, 672
TscAI	CASTG	1 cut(s)	631
TseFI	GTSAC	1 cut(s)	719
TseI	GCWGC	2 cut(s)	443, 446
Tsp45I	GTSAC	1 cut(s)	719
TspDTI	ATGAA	2 cut(s)	417, 730
TspRI	CASTG	1 cut(s)	631
Vha464I	CTTAAG	1 cut(s)	80
VpaK11BI	GGWCC	1 cut(s)	608
XapI	RAATTY	2 cut(s)	478, 736
XbaI	TCTAGA	1 cut(s)	346
XceI	RCATGY	2 cut(s)	357, 577
XmnI	GAANNNNTTC	1 cut(s)	408
XspI	CTAG	4 cut(s)	17, 347, 420, 440

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.