Rh5BG295000

Ribulose bisphosphate carboxylase, large chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006040
Physical Location & Seq
Forward (+)
37611932 .. 37618915
6984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG295000.1

Sequence Viewer

Length: 396 bp
ATGCCTGCTCTGACCGAGATCTTTGGAGATGATTCTGTACTACAATTCAGTGGAGGAACTTTAGGACACCCTTGGGGAAATGCACCAGGTGCCGTAGCTAATCGAGTAGCTCTAGAAGCATGTGTACAAGCTCGTAATGAGGGACGTGATCTCGCTCGTGAGGGTTTACCAGATTGTTTGGACAACTTCTTGGTTCTTTTAGAGAGAACAGGAGTAAATTCTCCCTCCTCTAAATCCTTCGTAGGCTCCAAATTTGGATCTAGGGACAATGGTGGATTAGACTTAGGCTCAAATGAAAGACTGTTGGTACTCGAAGAGTGTTCTATAGTCTCCATTTCGCGCCTCTGGAAGCCAGGAGGCACACTATCAGAGGAGGAAGCCGCAACCACCGACTGA

Protein Analysis

131

Amino Acids

13.83

Weight (kDa)

4.57

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuBisCO_large PF00016 1 - 54 4.8e-14 Ribulose bisphosphate carboxylase large chain, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 89
AccII CGCG 1 cut(s) 340
AciI CCGC 1 cut(s) 381
AclWI GGATC 1 cut(s) 265
AcsI RAATTY 2 cut(s) 217, 251
AdeI CACNNNGTG 1 cut(s) 89
AfaI GTAC 3 cut(s) 39, 126, 309
AjiI CACGTC 1 cut(s) 146
AjnI CCWGG 2 cut(s) 85, 352
AluBI AGCT 3 cut(s) 98, 110, 131
AluI AGCT 3 cut(s) 98, 110, 131
Alw26I GTCTC 1 cut(s) 334
AlwI GGATC 1 cut(s) 265
ApoI RAATTY 2 cut(s) 217, 251
AspLEI GCGC 1 cut(s) 342
BanI GGYRCC 1 cut(s) 89
BarI GAAGNNNNNNTAC 2 cut(s) 108, 140
BauI CACGAG 1 cut(s) 156
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 2 cut(s) 87, 354
BcoDI GTCTC 1 cut(s) 334
BfaI CTAG 2 cut(s) 113, 261
BfmI CTRYAG 1 cut(s) 324
BglII AGATCT 1 cut(s) 18
BisI GCNGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 382
Bme1390I CCNGG 2 cut(s) 87, 354
BmgBI CACGTC 1 cut(s) 146
BmiI GGNNCC 2 cut(s) 91, 247
BmrFI CCNGG 2 cut(s) 87, 354
BsaJI CCNNGG 1 cut(s) 71
BseBI CCWGG 2 cut(s) 87, 354
BseDI CCNNGG 1 cut(s) 71
BseRI GAGGAG 2 cut(s) 217, 386
Bsh1236I CGCG 1 cut(s) 340
BshNI GGYRCC 1 cut(s) 89
BslFI GGGAC 2 cut(s) 156, 278
BsmAI GTCTC 1 cut(s) 334
BsmFI GGGAC 2 cut(s) 156, 278
Bsp1407I TGTACA 1 cut(s) 124
Bsp143I GATC 3 cut(s) 18, 148, 257
BspACI CCGC 1 cut(s) 381
BspFNI CGCG 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 91, 247
BspPI GGATC 1 cut(s) 265
BspT107I GGYRCC 1 cut(s) 89
BsrGI TGTACA 1 cut(s) 124
BssECI CCNNGG 1 cut(s) 71
BssMI GATC 3 cut(s) 18, 148, 257
BssSI CACGAG 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 71
Bst2BI CACGAG 1 cut(s) 156
Bst2UI CCWGG 2 cut(s) 87, 354
Bst4CI ACNGT 1 cut(s) 303
Bst6I CTCTTC 1 cut(s) 309
BstAPI GCANNNNNTGC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 283
BstFNI CGCG 1 cut(s) 340
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 3 cut(s) 21, 151, 260
BstMAI GTCTC 1 cut(s) 334
BstMBI GATC 3 cut(s) 18, 148, 257
BstMWI GCNNNNNNNGC 2 cut(s) 89, 116
BstNI CCWGG 2 cut(s) 87, 354
BstNSI RCATGY 1 cut(s) 123
BstSCI CCNGG 2 cut(s) 85, 352
BstSFI CTRYAG 1 cut(s) 324
BstUI CGCG 1 cut(s) 340
BstX2I RGATCY 2 cut(s) 18, 257
BstYI RGATCY 2 cut(s) 18, 257
BtrI CACGTC 1 cut(s) 146
BtsIMutI CAGTG 1 cut(s) 55
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 342
CsiI ACCWGGT 1 cut(s) 85
Csp6I GTAC 3 cut(s) 38, 125, 308
CviAII CATG 1 cut(s) 120
CviJI RGCY 7 cut(s) 98, 110, 131, 246, 288, 352, 380
CviKI_1 RGCY 7 cut(s) 98, 110, 131, 246, 288, 352, 380
CviQI GTAC 3 cut(s) 38, 125, 308
DdeI CTNAG 1 cut(s) 283
DpnI GATC 3 cut(s) 20, 150, 259
DpnII GATC 3 cut(s) 18, 148, 257
DraIII CACNNNGTG 1 cut(s) 89
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 71
EcoRII CCWGG 2 cut(s) 85, 352
EcoT14I CCWWGG 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 71
FaeI CATG 1 cut(s) 123
FaiI YATR 2 cut(s) 121, 326
FaqI GGGAC 2 cut(s) 156, 278
FatI CATG 1 cut(s) 119
Fnu4HI GCNGC 1 cut(s) 381
Fsp4HI GCNGC 1 cut(s) 381
FspBI CTAG 2 cut(s) 113, 261
GlaI GCGC 1 cut(s) 341
GluI GCNGC 1 cut(s) 381
HhaI GCGC 1 cut(s) 342
Hin1II CATG 1 cut(s) 123
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HinfI GANTC 1 cut(s) 32
Hpy166II GTNNAC 2 cut(s) 125, 167
Hpy188I TCNGA 2 cut(s) 12, 370
Hpy188III TCNNGA 3 cut(s) 113, 158, 346
Hpy8I GTNNAC 2 cut(s) 125, 167
HpyAV CCTTC 1 cut(s) 247
HpyCH4III ACNGT 1 cut(s) 303
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 1 cut(s) 83
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 116
HpyF3I CTNAG 1 cut(s) 283
HpySE526I ACGT 1 cut(s) 145
Hsp92II CATG 1 cut(s) 123
HspAI GCGC 1 cut(s) 340
Kzo9I GATC 3 cut(s) 18, 148, 257
LmnI GCTCC 1 cut(s) 251
LpnPI CCDG 8 cut(s) 18, 72, 99, 183, 195, 331, 339, 366
MabI ACCWGGT 1 cut(s) 85
MaeI CTAG 2 cut(s) 113, 261
MaeII ACGT 1 cut(s) 145
MalI GATC 3 cut(s) 20, 150, 259
MboI GATC 3 cut(s) 18, 148, 257
MboII GAAGA 1 cut(s) 326
MflI RGATCY 2 cut(s) 18, 257
MluCI AATT 3 cut(s) 44, 217, 251
MnlI CCTC 9 cut(s) 47, 133, 154, 235, 238, 350, 353, 364, 367
MspR9I CCNGG 2 cut(s) 87, 354
MvaI CCWGG 2 cut(s) 87, 354
MvnI CGCG 1 cut(s) 340
MwoI GCNNNNNNNGC 2 cut(s) 89, 116
NdeII GATC 3 cut(s) 18, 148, 257
NlaIII CATG 1 cut(s) 123
NlaIV GGNNCC 2 cut(s) 91, 247
NspI RCATGY 1 cut(s) 123
PfeI GAWTC 1 cut(s) 32
PkrI GCNGC 1 cut(s) 382
Psp6I CCWGG 2 cut(s) 85, 352
PspGI CCWGG 2 cut(s) 85, 352
PspN4I GGNNCC 2 cut(s) 91, 247
PsuI RGATCY 2 cut(s) 18, 257
RsaI GTAC 3 cut(s) 39, 126, 309
RsaNI GTAC 3 cut(s) 38, 125, 308
SatI GCNGC 1 cut(s) 381
Sau3AI GATC 3 cut(s) 18, 148, 257
ScrFI CCNGG 2 cut(s) 87, 354
SetI ASST 5 cut(s) 91, 100, 112, 133, 148
SexAI ACCWGGT 1 cut(s) 85
SfcI CTRYAG 1 cut(s) 324
Sse9I AATT 3 cut(s) 44, 217, 251
SsiI CCGC 1 cut(s) 381
SspMI CTAG 2 cut(s) 113, 261
StyD4I CCNGG 2 cut(s) 85, 352
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 1 cut(s) 303
TaiI ACGT 1 cut(s) 148
TaqI TCGA 2 cut(s) 103, 312
TaqII GACCGA 1 cut(s) 29
TasI AATT 3 cut(s) 44, 217, 251
TatI WGTACW 2 cut(s) 37, 124
TauI GCSGC 1 cut(s) 383
TfiI GAWTC 1 cut(s) 32
TscAI CASTG 1 cut(s) 55
TspDTI ATGAA 1 cut(s) 309
TspRI CASTG 1 cut(s) 55
XapI RAATTY 2 cut(s) 217, 251
XbaI TCTAGA 1 cut(s) 112
XceI RCATGY 1 cut(s) 123
XspI CTAG 2 cut(s) 113, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.