RchiOBHm_Chr5g0043011

Ribulose bisphosphate carboxylase, large chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006040
Physical Location & Seq
Forward (+)
37820130 .. 37820399
270 bp
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UTR
Exon/CDS
Intron
PRQ32134

Sequence Viewer

Length: 270 bp
ATGCCTGCTCTGACCGAGATCTTTGGAGATGATTCTGTACTACAATTCAGTGGAGGAACTTTAGGACACCCTTGGGGAAATGCACCAGGTGCCGTAGCTAATCGAGTAGCTCTAGAAGCATGTGTACAAGCTCGTAATGAGGGACGTGATCTCGCTCGTGAGGGTAATGAAATTATTCGTGAGGCTAGTAAATGGAGTCCTGAACTAGCTGCCGCTTGTGAGGTATGGAAAGAGATCAAATTTGAATTCGAAGCAATGGATACTTTGTAA

Protein Analysis

89

Amino Acids

9.69

Weight (kDa)

4.54

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuBisCO_large PF00016 1 - 76 4.9e-19 Ribulose bisphosphate carboxylase large chain, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 89
AciI CCGC 1 cut(s) 213
AcsI RAATTY 2 cut(s) 239, 245
AdeI CACNNNGTG 1 cut(s) 89
AfaI GTAC 2 cut(s) 39, 126
AgsI TTSAA 1 cut(s) 245
AjiI CACGTC 1 cut(s) 146
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 4 cut(s) 98, 110, 131, 209
AluI AGCT 4 cut(s) 98, 110, 131, 209
ApeKI GCWGC 1 cut(s) 209
ApoI RAATTY 2 cut(s) 239, 245
Asp700I GAANNNNTTC 1 cut(s) 174
AsuII TTCGAA 1 cut(s) 249
BanI GGYRCC 1 cut(s) 89
BarI GAAGNNNNNNTAC 2 cut(s) 108, 140
BauI CACGAG 1 cut(s) 156
BbvI GCAGC 1 cut(s) 196
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 87
BciVI GTATCC 1 cut(s) 253
BfaI CTAG 3 cut(s) 113, 186, 206
BfuI GTATCC 1 cut(s) 253
BglII AGATCT 1 cut(s) 18
BisI GCNGC 2 cut(s) 210, 213
BlsI GCNGC 2 cut(s) 211, 214
Bme1390I CCNGG 1 cut(s) 87
BmgBI CACGTC 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 1 cut(s) 87
Bpu14I TTCGAA 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 71
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 71
BseMI GCAATG 1 cut(s) 261
BseXI GCAGC 1 cut(s) 196
BshNI GGYRCC 1 cut(s) 89
BslFI GGGAC 1 cut(s) 156
BsmFI GGGAC 1 cut(s) 156
Bsp119I TTCGAA 1 cut(s) 249
Bsp1407I TGTACA 1 cut(s) 124
Bsp143I GATC 3 cut(s) 18, 148, 234
BspACI CCGC 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 91
BspT104I TTCGAA 1 cut(s) 249
BspT107I GGYRCC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 261
BsrGI TGTACA 1 cut(s) 124
BssECI CCNNGG 1 cut(s) 71
BssMI GATC 3 cut(s) 18, 148, 234
BssSI CACGAG 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 71
Bst2BI CACGAG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 87
BstAPI GCANNNNNTGC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 124
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 6
BstKTI GATC 3 cut(s) 21, 151, 237
BstMBI GATC 3 cut(s) 18, 148, 234
BstMWI GCNNNNNNNGC 2 cut(s) 89, 116
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 196
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
BsuI GTATCC 1 cut(s) 253
BtrI CACGTC 1 cut(s) 146
BtsIMutI CAGTG 1 cut(s) 55
Cac8I GCNNGC 1 cut(s) 6
CsiI ACCWGGT 1 cut(s) 85
Csp6I GTAC 2 cut(s) 38, 125
CviAII CATG 1 cut(s) 120
CviJI RGCY 5 cut(s) 98, 110, 131, 185, 209
CviKI_1 RGCY 5 cut(s) 98, 110, 131, 185, 209
CviQI GTAC 2 cut(s) 38, 125
DpnI GATC 3 cut(s) 20, 150, 236
DpnII GATC 3 cut(s) 18, 148, 234
DraIII CACNNNGTG 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 71
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 71
FaeI CATG 1 cut(s) 123
FaiI YATR 2 cut(s) 121, 226
FaqI GGGAC 1 cut(s) 156
FatI CATG 1 cut(s) 119
Fnu4HI GCNGC 2 cut(s) 210, 213
Fsp4HI GCNGC 2 cut(s) 210, 213
FspBI CTAG 3 cut(s) 113, 186, 206
GluI GCNGC 2 cut(s) 210, 213
Hin1II CATG 1 cut(s) 123
HinfI GANTC 2 cut(s) 32, 196
Hpy166II GTNNAC 1 cut(s) 125
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 4 cut(s) 113, 158, 179, 200
Hpy8I GTNNAC 1 cut(s) 125
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 1 cut(s) 83
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 116
HpySE526I ACGT 1 cut(s) 145
Hsp92II CATG 1 cut(s) 123
Kzo9I GATC 3 cut(s) 18, 148, 234
LpnPI CCDG 4 cut(s) 18, 72, 99, 213
Lsp1109I GCAGC 1 cut(s) 196
MabI ACCWGGT 1 cut(s) 85
MaeI CTAG 3 cut(s) 113, 186, 206
MaeII ACGT 1 cut(s) 145
MalI GATC 3 cut(s) 20, 150, 236
MboI GATC 3 cut(s) 18, 148, 234
MflI RGATCY 1 cut(s) 18
MluCI AATT 4 cut(s) 44, 171, 239, 245
MlyI GAGTC 1 cut(s) 205
MnlI CCTC 5 cut(s) 47, 133, 154, 175, 214
MroXI GAANNNNTTC 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 89, 116
NdeII GATC 3 cut(s) 18, 148, 234
NlaIII CATG 1 cut(s) 123
NlaIV GGNNCC 1 cut(s) 91
NspI RCATGY 1 cut(s) 123
NspV TTCGAA 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 1 cut(s) 32
PkrI GCNGC 2 cut(s) 211, 214
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 85
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 1 cut(s) 91
PsuI RGATCY 1 cut(s) 18
RsaI GTAC 2 cut(s) 39, 126
RsaNI GTAC 2 cut(s) 38, 125
SatI GCNGC 2 cut(s) 210, 213
Sau3AI GATC 3 cut(s) 18, 148, 234
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 1 cut(s) 87
SetI ASST 7 cut(s) 91, 100, 112, 133, 148, 211, 225
SexAI ACCWGGT 1 cut(s) 85
SfuI TTCGAA 1 cut(s) 249
Sse9I AATT 4 cut(s) 44, 171, 239, 245
SsiI CCGC 1 cut(s) 213
SspMI CTAG 3 cut(s) 113, 186, 206
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 1 cut(s) 71
TaiI ACGT 1 cut(s) 148
TaqI TCGA 2 cut(s) 103, 249
TaqII GACCGA 1 cut(s) 29
TasI AATT 4 cut(s) 44, 171, 239, 245
TatI WGTACW 2 cut(s) 37, 124
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 1 cut(s) 32
TscAI CASTG 1 cut(s) 55
TseI GCWGC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 183
TspRI CASTG 1 cut(s) 55
XapI RAATTY 2 cut(s) 239, 245
XbaI TCTAGA 1 cut(s) 112
XceI RCATGY 1 cut(s) 123
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 3 cut(s) 113, 186, 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.