MD02G1057400.v1.1

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008537
Physical Location & Seq
Forward (+)
4637397 .. 4639705
2309 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1057400.v1.1.491

Sequence Viewer

Length: 627 bp
ATGAAGCTGAAGCAGTTGGAAGGCCACCTTGGCGGTCTCCAACAGTTCTCCAATCCAAAGGTGGAGCTGGAGCAATACCCAACTGGACCCCACATTGCTTCTCGCATGCTCTTCACTGCGGAGAATTCATTCGGAGACGTGAGTGGGAAGGTAGTGGCTGATTTCGGTTGCGGTTGTGGCACATTGGGTGTTGCAGCTGCACTCTTGGGCGCAGAACAGGTGATTGGCATTGACATCGATGCTCAGTCGCTTGAAATAGCAGCGGAAAATGCCGAGGATCTTGAGTTGGACATGGATTTTATTCAGTGCAACATTAAGAACTTAGGATGGAGAGCTAGCGTTGTTGATACTGTCGTAATGAATCCTCCATTTGGAACTCGGAATAAGGGTGCTGACATGGATTTCCTCTCAATAGCTTTGAAGGTTGCTTCTCAAGCAGTTTATTCCTTGCACAAGACTTCCACGAGAGATCATGTGAAAAGGGCAGCCTTGCAGAACTTCAGTGCGAGCAGTGCTGAAGTTATATGTGAGCTTCGATACGATGTACCACAGCTGTACAAATTTCACAAGAAAAGGGAGGTGGATATTGCCGTGGACTTGTGGCGATTCGTTCCCAGGAGTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS PrmA

Protein Analysis

209

Amino Acids

22.81

Weight (kDa)

5.54

Isoelectric Point (pI)

32.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PrmA PF06325 28 - 121 9.7e-14 Ribosomal protein L11 methyltransferase (PrmA)
Cons_hypoth95 PF03602 47 - 137 3.2e-06 Conserved hypothetical protein 95
MTS PF05175 48 - 129 2e-13 Methyltransferase small domain
Methyltransf_31 PF13847 48 - 133 1.2e-12 Methyltransferase domain
UPF0020 PF01170 49 - 129 3.3e-07 RMKL-like, methyltransferase domain
Methyltransf_25 PF13649 52 - 121 1.9e-11 Methyltransferase domain
Methyltransf_11 PF08241 54 - 121 5.7e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 33, 119, 171, 263
AclWI GGATC 1 cut(s) 285
AcsI RAATTY 2 cut(s) 124, 560
AcuI CTGAAG 3 cut(s) 29, 484, 537
AfaI GTAC 2 cut(s) 546, 557
AfiI CCNNNNNNNGG 1 cut(s) 371
AgsI TTSAA 2 cut(s) 254, 421
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 1 cut(s) 614
AjuI GAANNNNNNNTTGG 4 cut(s) 12, 44, 207, 239
AluBI AGCT 7 cut(s) 7, 67, 197, 335, 416, 532, 553
AluI AGCT 7 cut(s) 7, 67, 197, 335, 416, 532, 553
Alw26I GTCTC 2 cut(s) 41, 129
AlwI GGATC 1 cut(s) 285
AoxI GGCC 1 cut(s) 22
ApeKI GCWGC 4 cut(s) 194, 197, 260, 485
ApoI RAATTY 2 cut(s) 124, 560
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 232
AsuNHI GCTAGC 1 cut(s) 335
AvaII GGWCC 1 cut(s) 86
BaeI ACNNNNGTAYC 2 cut(s) 339, 372
BauI CACGAG 1 cut(s) 463
BbvI GCAGC 4 cut(s) 184, 206, 272, 497
BccI CCATC 1 cut(s) 321
BceAI ACGGC 1 cut(s) 575
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 1 cut(s) 616
BcoDI GTCTC 2 cut(s) 41, 129
BfaI CTAG 2 cut(s) 336, 625
BglI GCCNNNNNGGC 1 cut(s) 30
BisI GCNGC 4 cut(s) 195, 198, 261, 486
BlsI GCNGC 4 cut(s) 196, 199, 262, 487
Bme1390I CCNGG 1 cut(s) 616
Bme18I GGWCC 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 616
BmsI GCATC 1 cut(s) 229
BmtI GCTAGC 1 cut(s) 339
BpmI CTGGAG 1 cut(s) 89
BpuEI CTTGAG 2 cut(s) 302, 417
Bsa29I ATCGAT 1 cut(s) 237
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 4 cut(s) 28, 273, 591, 614
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 93
BseBI CCWGG 1 cut(s) 616
BseCI ATCGAT 1 cut(s) 237
BseDI CCNNGG 4 cut(s) 28, 273, 591, 614
BseGI GGATG 1 cut(s) 332
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 1 cut(s) 88
BseXI GCAGC 4 cut(s) 184, 206, 272, 497
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 1 cut(s) 24
BshVI ATCGAT 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 371
BsmAI GTCTC 2 cut(s) 41, 129
BsmBI CGTCTC 1 cut(s) 129
BsnI GGCC 1 cut(s) 24
Bso31I GGTCTC 1 cut(s) 41
Bsp1407I TGTACA 1 cut(s) 555
Bsp143I GATC 2 cut(s) 277, 469
BspACI CCGC 4 cut(s) 33, 119, 171, 263
BspANI GGCC 1 cut(s) 24
BspCNI CTCAG 1 cut(s) 256
BspDI ATCGAT 1 cut(s) 237
BspLI GGNNCC 1 cut(s) 88
BspOI GCTAGC 1 cut(s) 339
BspPI GGATC 1 cut(s) 285
BspQI GCTCTTC 1 cut(s) 116
BspTNI GGTCTC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 93
BsrGI TGTACA 1 cut(s) 555
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 4 cut(s) 28, 273, 591, 614
BssMI GATC 2 cut(s) 277, 469
BssSI CACGAG 1 cut(s) 463
BssT1I CCWWGG 1 cut(s) 28
Bst2BI CACGAG 1 cut(s) 463
Bst2UI CCWGG 1 cut(s) 616
Bst4CI ACNGT 2 cut(s) 45, 352
Bst6I CTCTTC 1 cut(s) 116
BstAUI TGTACA 1 cut(s) 555
BstC8I GCNNGC 3 cut(s) 107, 337, 508
BstDEI CTNAG 2 cut(s) 243, 322
BstDSI CCRYGG 1 cut(s) 591
BstF5I GGATG 1 cut(s) 332
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 2 cut(s) 280, 472
BstMAI GTCTC 2 cut(s) 41, 129
BstMBI GATC 2 cut(s) 277, 469
BstMWI GCNNNNNNNGC 5 cut(s) 30, 177, 269, 434, 512
BstNI CCWGG 1 cut(s) 616
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 614
BstV1I GCAGC 4 cut(s) 184, 206, 272, 497
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 237
BsuRI GGCC 1 cut(s) 24
BsuTUI ATCGAT 1 cut(s) 237
BtgI CCRYGG 1 cut(s) 591
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 1 cut(s) 332
BtsI GCAGTG 2 cut(s) 114, 517
BtsIMutI CAGTG 4 cut(s) 114, 311, 508, 517
Cac8I GCNNGC 3 cut(s) 107, 337, 508
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 86
ClaI ATCGAT 1 cut(s) 237
Csp6I GTAC 2 cut(s) 545, 556
CviAII CATG 4 cut(s) 106, 292, 397, 473
CviQI GTAC 2 cut(s) 545, 556
DdeI CTNAG 2 cut(s) 243, 322
DpnI GATC 2 cut(s) 279, 471
DpnII GATC 2 cut(s) 277, 469
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 28
Eco31I GGTCTC 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 86
Eco57I CTGAAG 3 cut(s) 29, 484, 537
EcoRI GAATTC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 614
EcoT14I CCWWGG 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 28
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 4 cut(s) 109, 295, 400, 476
FaiI YATR 6 cut(s) 107, 293, 398, 474, 524, 526
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FatI CATG 4 cut(s) 105, 291, 396, 472
Fnu4HI GCNGC 4 cut(s) 195, 198, 261, 486
FokI GGATG 1 cut(s) 339
Fsp4HI GCNGC 4 cut(s) 195, 198, 261, 486
FspBI CTAG 2 cut(s) 336, 625
GlaI GCGC 1 cut(s) 211
GluI GCNGC 4 cut(s) 195, 198, 261, 486
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 1 cut(s) 24
HhaI GCGC 1 cut(s) 212
Hin1II CATG 4 cut(s) 109, 295, 400, 476
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HinfI GANTC 2 cut(s) 361, 606
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 1 cut(s) 595
Hpy188I TCNGA 2 cut(s) 134, 381
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 1 cut(s) 595
HpyAV CCTTC 3 cut(s) 14, 142, 415
HpyCH4III ACNGT 2 cut(s) 45, 352
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 5 cut(s) 194, 200, 309, 451, 493
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 177, 269, 434, 512
HpyF3I CTNAG 2 cut(s) 243, 322
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 4 cut(s) 109, 295, 400, 476
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 2 cut(s) 277, 469
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 2 cut(s) 64, 70
LpnPI CCDG 4 cut(s) 53, 69, 203, 601
Lsp1109I GCAGC 4 cut(s) 184, 206, 272, 497
LweI GCATC 1 cut(s) 229
MaeI CTAG 2 cut(s) 336, 625
MaeII ACGT 1 cut(s) 138
MalI GATC 2 cut(s) 279, 471
MboI GATC 2 cut(s) 277, 469
MboII GAAGA 1 cut(s) 103
MflI RGATCY 1 cut(s) 277
MluCI AATT 2 cut(s) 124, 560
MmeI TCCRAC 2 cut(s) 64, 267
MnlI CCTC 4 cut(s) 268, 375, 416, 571
MroXI GAANNNNTTC 1 cut(s) 128
MseI TTAA 1 cut(s) 315
MspA1I CMGCKG 3 cut(s) 197, 263, 553
MspR9I CCNGG 1 cut(s) 616
MvaI CCWGG 1 cut(s) 616
MwoI GCNNNNNNNGC 5 cut(s) 30, 177, 269, 434, 512
NdeII GATC 2 cut(s) 277, 469
NheI GCTAGC 1 cut(s) 335
NlaIII CATG 4 cut(s) 109, 295, 400, 476
NlaIV GGNNCC 1 cut(s) 88
NmeAIII GCCGAG 1 cut(s) 298
NspI RCATGY 1 cut(s) 109
PaeI GCATGC 1 cut(s) 109
PciSI GCTCTTC 1 cut(s) 116
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 361, 606
PkrI GCNGC 4 cut(s) 196, 199, 262, 487
Psp6I CCWGG 1 cut(s) 614
PspGI CCWGG 1 cut(s) 614
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 1 cut(s) 86
PsuI RGATCY 1 cut(s) 277
PvuII CAGCTG 2 cut(s) 197, 553
RsaI GTAC 2 cut(s) 546, 557
RsaNI GTAC 2 cut(s) 545, 556
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 4 cut(s) 195, 198, 261, 486
Sau3AI GATC 2 cut(s) 277, 469
Sau96I GGNCC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 616
SfaNI GCATC 1 cut(s) 229
SinI GGWCC 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 281, 432
SmoI CTYRAG 2 cut(s) 281, 432
SphI GCATGC 1 cut(s) 109
Sse9I AATT 2 cut(s) 124, 560
SsiI CCGC 4 cut(s) 33, 119, 171, 263
SspMI CTAG 2 cut(s) 336, 625
StyD4I CCNGG 1 cut(s) 614
StyI CCWWGG 1 cut(s) 28
TaaI ACNGT 2 cut(s) 45, 352
TaiI ACGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 237, 535
TasI AATT 2 cut(s) 124, 560
TatI WGTACW 1 cut(s) 555
TfiI GAWTC 2 cut(s) 361, 606
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 4 cut(s) 121, 311, 508, 517
TseI GCWGC 4 cut(s) 194, 197, 260, 485
TspDTI ATGAA 3 cut(s) 17, 117, 374
TspRI CASTG 4 cut(s) 121, 311, 508, 517
VpaK11BI GGWCC 1 cut(s) 86
XapI RAATTY 2 cut(s) 124, 560
XceI RCATGY 1 cut(s) 109
XcmI CCANNNNNNNNNTGG 1 cut(s) 58
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 2 cut(s) 336, 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.