Rh2BG059900

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008537
Physical Location & Seq
Forward (+)
4497174 .. 4499604
2431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG059900.1

Sequence Viewer

Length: 534 bp
ATGAAGCTCAAGCAGCTCGAAAGCCTCCTTGGTGGTCTCCAACAGTTCTCCAACCCAAAGGTGGAGCTGGAACAATACCCAACTGGACCCCACATTGCATCTCGCATGCTCTACACCGCAGAGAATTCATTTGGGGATGTGTGTGACAAGGTAGTGGCTGATTTTGGCTGTGGTTGTGGTACATTGGGGGTTGCAGCTGGACTCTTGGGCGCAGAACATGTGATTGGCATTGACGTCGATCCAGAATCTCTTGAATTAGCTTCACTAAATGCTGAGGAACTTGAGTTGGACATAGATTATGTTCAATGCAACATCAAGAACTTAGGGTGGAGAGCTGAAGTTGTTGATACTATTGTAATGAATCCTCCATTTGGAACTCGGAAAAAGGGTGCTGACATGGATTTTCTCTCTGTGGCTTTGAAGGTTGCTTCTCAAGCTGTTTATTCCTTACATAAAACCTCCACGAGAGATGTAAGTCTTTTGCTGCAGAATATTTTGTTACTTAGAAGGATTATTTACTGCGAGTACATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS PrmA

Protein Analysis

177

Amino Acids

19.39

Weight (kDa)

4.82

Isoelectric Point (pI)

28.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PrmA PF06325 28 - 121 3.6e-11 Ribosomal protein L11 methyltransferase (PrmA)
MTS PF05175 48 - 129 2.1e-12 Methyltransferase small domain
UPF0020 PF01170 49 - 130 7.4e-08 RMKL-like, methyltransferase domain
Methyltransf_31 PF13847 50 - 124 7.4e-09 Methyltransferase domain
Methyltransf_25 PF13649 52 - 121 3.5e-10 Methyltransferase domain
Methyltransf_11 PF08241 54 - 121 1.2e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 237
AciI CCGC 1 cut(s) 117
AclWI GGATC 1 cut(s) 233
AcsI RAATTY 1 cut(s) 124
AcuI CTGAAG 1 cut(s) 357
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 2 cut(s) 181, 527
AfiI CCNNNNNNNGG 2 cut(s) 61, 371
AflIII ACRYGT 1 cut(s) 217
AgsI TTSAA 3 cut(s) 254, 305, 421
AjuI GAANNNNNNNTTGG 4 cut(s) 12, 44, 207, 239
AluBI AGCT 7 cut(s) 7, 16, 67, 197, 260, 335, 437
AluI AGCT 7 cut(s) 7, 16, 67, 197, 260, 335, 437
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 1 cut(s) 233
ApeKI GCWGC 3 cut(s) 13, 194, 484
ApoI RAATTY 1 cut(s) 124
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 86
AvaII GGWCC 1 cut(s) 86
BaeI ACNNNNGTAYC 2 cut(s) 339, 372
BauI CACGAG 1 cut(s) 463
BbvCI CCTCAGC 1 cut(s) 273
BbvI GCAGC 3 cut(s) 25, 206, 471
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 1 cut(s) 532
BfmI CTRYAG 1 cut(s) 485
BisI GCNGC 3 cut(s) 14, 195, 485
BlsI GCNGC 3 cut(s) 15, 196, 486
Bme18I GGWCC 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 1 cut(s) 88
BmsI GCATC 1 cut(s) 107
Bpu10I CCTNAGC 1 cut(s) 273
BpuEI CTTGAG 2 cut(s) 302, 417
BsaHI GRCGYC 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 28
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 371
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 1 cut(s) 142
BseLI CCNNNNNNNGG 2 cut(s) 61, 371
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 88
BseXI GCAGC 3 cut(s) 25, 206, 471
BslI CCNNNNNNNGG 2 cut(s) 61, 371
BsmAI GTCTC 1 cut(s) 41
Bso31I GGTCTC 1 cut(s) 41
Bsp143I GATC 1 cut(s) 238
BspACI CCGC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 88
BspMAI CTGCAG 1 cut(s) 489
BspPI GGATC 1 cut(s) 233
BspTNI GGTCTC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 93
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 1 cut(s) 238
BssNI GRCGYC 1 cut(s) 234
BssSI CACGAG 1 cut(s) 463
BssT1I CCWWGG 1 cut(s) 28
Bst2BI CACGAG 1 cut(s) 463
Bst4CI ACNGT 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 3 cut(s) 273, 322, 503
BstF5I GGATG 1 cut(s) 142
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 1 cut(s) 241
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 1 cut(s) 238
BstMWI GCNNNNNNNGC 2 cut(s) 13, 434
BstNSI RCATGY 2 cut(s) 109, 221
BstSFI CTRYAG 1 cut(s) 485
BstV1I GCAGC 3 cut(s) 25, 206, 471
BtsCI GGATG 1 cut(s) 142
Cac8I GCNNGC 1 cut(s) 107
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 86
Csp6I GTAC 2 cut(s) 180, 526
CviAII CATG 3 cut(s) 106, 218, 397
CviQI GTAC 2 cut(s) 180, 526
DdeI CTNAG 3 cut(s) 273, 322, 503
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 28
Eco31I GGTCTC 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 357
EcoRI GAATTC 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 28
FaeI CATG 3 cut(s) 109, 221, 400
FaiI YATR 6 cut(s) 107, 219, 293, 300, 398, 453
FatI CATG 3 cut(s) 105, 217, 396
Fnu4HI GCNGC 3 cut(s) 14, 195, 485
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 3 cut(s) 14, 195, 485
FspBI CTAG 1 cut(s) 532
GlaI GCGC 1 cut(s) 211
GluI GCNGC 3 cut(s) 14, 195, 485
HhaI GCGC 1 cut(s) 212
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 3 cut(s) 109, 221, 400
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HinfI GANTC 3 cut(s) 201, 245, 361
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 3 cut(s) 242, 251, 316
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 2 cut(s) 415, 501
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 4 cut(s) 98, 194, 309, 487
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 434
HpyF3I CTNAG 3 cut(s) 273, 322, 503
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 3 cut(s) 109, 221, 400
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 1 cut(s) 238
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 4 cut(s) 53, 69, 183, 255
Lsp1109I GCAGC 3 cut(s) 25, 206, 471
LweI GCATC 1 cut(s) 107
MaeI CTAG 1 cut(s) 532
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 2 cut(s) 143, 498
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MluCI AATT 2 cut(s) 124, 254
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 3 cut(s) 64, 75, 267
MnlI CCTC 4 cut(s) 35, 268, 375, 469
MspA1I CMGCKG 1 cut(s) 197
MwoI GCNNNNNNNGC 2 cut(s) 13, 434
NdeII GATC 1 cut(s) 238
NlaIII CATG 3 cut(s) 109, 221, 400
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 143
NspI RCATGY 2 cut(s) 109, 221
PaeI GCATGC 1 cut(s) 109
PciI ACATGT 1 cut(s) 217
PfeI GAWTC 2 cut(s) 245, 361
PkrI GCNGC 3 cut(s) 15, 196, 486
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
PscI ACATGT 1 cut(s) 217
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 1 cut(s) 86
PstI CTGCAG 1 cut(s) 489
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 2 cut(s) 181, 527
RsaNI GTAC 2 cut(s) 180, 526
SatI GCNGC 3 cut(s) 14, 195, 485
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 1 cut(s) 86
SchI GAGTC 1 cut(s) 195
SfaNI GCATC 1 cut(s) 107
SfcI CTRYAG 1 cut(s) 485
SinI GGWCC 1 cut(s) 86
SmlI CTYRAG 3 cut(s) 8, 281, 432
SmoI CTYRAG 3 cut(s) 8, 281, 432
SphI GCATGC 1 cut(s) 109
Sse9I AATT 2 cut(s) 124, 254
SsiI CCGC 1 cut(s) 117
SspI AATATT 1 cut(s) 493
SspMI CTAG 1 cut(s) 532
StyI CCWWGG 1 cut(s) 28
TaaI ACNGT 1 cut(s) 45
TaiI ACGT 1 cut(s) 237
TaqI TCGA 2 cut(s) 18, 237
TasI AATT 2 cut(s) 124, 254
TatI WGTACW 1 cut(s) 525
TfiI GAWTC 2 cut(s) 245, 361
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 3 cut(s) 13, 194, 484
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 3 cut(s) 17, 117, 374
VpaK11BI GGWCC 1 cut(s) 86
XapI RAATTY 1 cut(s) 124
XceI RCATGY 2 cut(s) 109, 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 58
XspI CTAG 1 cut(s) 532
ZraI GACGTC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.