RchiOBHm_Chr2g0091271

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008537
Physical Location & Seq
Forward (+)
4941206 .. 4943385
2180 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46646

Sequence Viewer

Length: 642 bp
ATGAAGCTCAAGCAGCTCGAAAGCCTCCTTGGTGGTCTCCAACAGTTCTCCAACCCAAAGGTGGAGCTGGAACAATACCCAACTGGACCCCACATTGCATCTCGCATGCTCTACACTGCAGAGAATTCATTTGGGGATGTGTGTGACAAGGTAGTGGCTGATTTTGGCTGTGGTTGTGGTACATTGGGGGTTGCAGCTGGACTCTTGGGCGCAGAACATGTGATTGGCATTGACGTCGATCCAGAATCTCTTGAATTAGCTTCACTAAATGCTGAGGAACTTGAGTTGGACATAGATTATGTTCAATGCAACATCAAGAACTTAGGGTGGAGAGCTGAAGTTGTTGATACTATTGTAATGAATCCTCCATTTGGAACTCGGAAAAAGGGTGCTGACATGGATTTTCTCTCTGTGGCTTTGAAGGTTGCTTCTCAAGCTGTTTATTCCTTACATAAAACCTCCACGAGAGATCATGTGAAAAGGGCAGCCTTGCAGCAGTTCAATGCTAGCAGTGCTGAGGTTATATGTGAGCTTCGATATGATTTACCACAAACGTACAAGTTTCATAAGAAACGAGAGGTGGATATCGCTGTGGACTTGTGGCGATTTGTCCCCAAGAGTAAGCAGGGTCCAGAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS PrmA

Protein Analysis

213

Amino Acids

23.51

Weight (kDa)

5.41

Isoelectric Point (pI)

30.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PrmA PF06325 28 - 121 5.6e-11 Ribosomal protein L11 methyltransferase (PrmA)
MTS PF05175 48 - 130 2.8e-12 Methyltransferase small domain
Methyltransf_31 PF13847 50 - 124 8.8e-09 Methyltransferase domain
UPF0020 PF01170 50 - 129 1.2e-07 RMKL-like, methyltransferase domain
Methyltransf_25 PF13649 52 - 121 5.3e-10 Methyltransferase domain
Methyltransf_11 PF08241 54 - 121 1.7e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 237
AclWI GGATC 1 cut(s) 233
AcsI RAATTY 1 cut(s) 124
AcuI CTGAAG 1 cut(s) 357
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 2 cut(s) 181, 557
AfiI CCNNNNNNNGG 2 cut(s) 61, 371
AflIII ACRYGT 1 cut(s) 217
AgsI TTSAA 4 cut(s) 254, 305, 421, 502
AjuI GAANNNNNNNTTGG 4 cut(s) 12, 44, 207, 239
AluBI AGCT 8 cut(s) 7, 16, 67, 197, 260, 335, 437, 532
AluI AGCT 8 cut(s) 7, 16, 67, 197, 260, 335, 437, 532
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 1 cut(s) 233
ApeKI GCWGC 4 cut(s) 13, 194, 485, 493
ApoI RAATTY 1 cut(s) 124
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 86, 629
AsuNHI GCTAGC 1 cut(s) 506
AvaII GGWCC 2 cut(s) 86, 629
BaeI ACNNNNGTAYC 2 cut(s) 339, 372
BauI CACGAG 1 cut(s) 463
BbvCI CCTCAGC 2 cut(s) 273, 516
BbvI GCAGC 4 cut(s) 25, 206, 497, 505
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 2 cut(s) 507, 640
BfmI CTRYAG 1 cut(s) 117
BisI GCNGC 4 cut(s) 14, 195, 486, 494
BlsI GCNGC 4 cut(s) 15, 196, 487, 495
Bme18I GGWCC 2 cut(s) 86, 629
BmgT120I GGNCC 2 cut(s) 86, 629
BmiI GGNNCC 2 cut(s) 88, 630
BmsI GCATC 1 cut(s) 107
BmtI GCTAGC 1 cut(s) 510
Bpu10I CCTNAGC 2 cut(s) 273, 516
BpuEI CTTGAG 2 cut(s) 302, 417
BsaHI GRCGYC 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 28
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 371
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 1 cut(s) 142
BseLI CCNNNNNNNGG 2 cut(s) 61, 371
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 2 cut(s) 264, 507
BseNI ACTGG 1 cut(s) 88
BseXI GCAGC 4 cut(s) 25, 206, 497, 505
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 2 cut(s) 61, 371
BsmAI GTCTC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 596
Bso31I GGTCTC 1 cut(s) 41
Bsp143I GATC 2 cut(s) 238, 469
BspCNI CTCAG 2 cut(s) 265, 508
BspLI GGNNCC 2 cut(s) 88, 630
BspMAI CTGCAG 1 cut(s) 121
BspOI GCTAGC 1 cut(s) 510
BspPI GGATC 1 cut(s) 233
BspTNI GGTCTC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 93
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 2 cut(s) 238, 469
BssNI GRCGYC 1 cut(s) 234
BssSI CACGAG 1 cut(s) 463
BssT1I CCWWGG 1 cut(s) 28
Bst2BI CACGAG 1 cut(s) 463
Bst4CI ACNGT 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 234
BstC8I GCNNGC 2 cut(s) 107, 508
BstDEI CTNAG 3 cut(s) 273, 322, 516
BstF5I GGATG 1 cut(s) 142
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 2 cut(s) 241, 472
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 2 cut(s) 238, 469
BstMWI GCNNNNNNNGC 3 cut(s) 13, 434, 512
BstNSI RCATGY 2 cut(s) 109, 221
BstSFI CTRYAG 1 cut(s) 117
BstV1I GCAGC 4 cut(s) 25, 206, 497, 505
BtsCI GGATG 1 cut(s) 142
BtsI GCAGTG 2 cut(s) 114, 517
BtsIMutI CAGTG 2 cut(s) 114, 517
Cac8I GCNNGC 2 cut(s) 107, 508
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 86, 629
Csp6I GTAC 2 cut(s) 180, 556
CviAII CATG 4 cut(s) 106, 218, 397, 473
CviQI GTAC 2 cut(s) 180, 556
DdeI CTNAG 3 cut(s) 273, 322, 516
DpnI GATC 2 cut(s) 240, 471
DpnII GATC 2 cut(s) 238, 469
Eco130I CCWWGG 1 cut(s) 28
Eco31I GGTCTC 1 cut(s) 41
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 2 cut(s) 86, 629
Eco57I CTGAAG 1 cut(s) 357
EcoRI GAATTC 1 cut(s) 124
EcoRV GATATC 1 cut(s) 586
EcoT14I CCWWGG 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 28
FaeI CATG 4 cut(s) 109, 221, 400, 476
FaqI GGGAC 1 cut(s) 596
FatI CATG 4 cut(s) 105, 217, 396, 472
Fnu4HI GCNGC 4 cut(s) 14, 195, 486, 494
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 4 cut(s) 14, 195, 486, 494
FspBI CTAG 2 cut(s) 507, 640
GlaI GCGC 1 cut(s) 211
GluI GCNGC 4 cut(s) 14, 195, 486, 494
HhaI GCGC 1 cut(s) 212
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 4 cut(s) 109, 221, 400, 476
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HinfI GANTC 3 cut(s) 201, 245, 361
Hpy166II GTNNAC 1 cut(s) 595
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 4 cut(s) 242, 251, 316, 632
Hpy8I GTNNAC 1 cut(s) 595
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 415
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4IV ACGT 2 cut(s) 234, 554
HpyCH4V TGCA 5 cut(s) 98, 119, 194, 309, 493
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 434, 512
HpyF3I CTNAG 3 cut(s) 273, 322, 516
HpySE526I ACGT 2 cut(s) 234, 554
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 4 cut(s) 109, 221, 400, 476
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 2 cut(s) 238, 469
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 5 cut(s) 53, 69, 183, 255, 611
Lsp1109I GCAGC 4 cut(s) 25, 206, 497, 505
LweI GCATC 1 cut(s) 107
MaeI CTAG 2 cut(s) 507, 640
MaeII ACGT 2 cut(s) 234, 554
MaeIII GTNAC 1 cut(s) 143
MalI GATC 2 cut(s) 240, 471
MboI GATC 2 cut(s) 238, 469
MluCI AATT 2 cut(s) 124, 254
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 3 cut(s) 64, 75, 267
MnlI CCTC 7 cut(s) 35, 268, 375, 469, 511, 571, 628
MspA1I CMGCKG 1 cut(s) 197
MwoI GCNNNNNNNGC 3 cut(s) 13, 434, 512
NdeII GATC 2 cut(s) 238, 469
NheI GCTAGC 1 cut(s) 506
NlaIII CATG 4 cut(s) 109, 221, 400, 476
NlaIV GGNNCC 2 cut(s) 88, 630
NmuCI GTSAC 1 cut(s) 143
NspI RCATGY 2 cut(s) 109, 221
PaeI GCATGC 1 cut(s) 109
PciI ACATGT 1 cut(s) 217
PfeI GAWTC 2 cut(s) 245, 361
PkrI GCNGC 4 cut(s) 15, 196, 487, 495
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
PscI ACATGT 1 cut(s) 217
PspN4I GGNNCC 2 cut(s) 88, 630
PspPI GGNCC 2 cut(s) 86, 629
PstI CTGCAG 1 cut(s) 121
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 2 cut(s) 181, 557
RsaNI GTAC 2 cut(s) 180, 556
SatI GCNGC 4 cut(s) 14, 195, 486, 494
Sau3AI GATC 2 cut(s) 238, 469
Sau96I GGNCC 2 cut(s) 86, 629
SchI GAGTC 1 cut(s) 195
SfaNI GCATC 1 cut(s) 107
SfcI CTRYAG 1 cut(s) 117
SinI GGWCC 2 cut(s) 86, 629
SmlI CTYRAG 3 cut(s) 8, 281, 432
SmoI CTYRAG 3 cut(s) 8, 281, 432
SphI GCATGC 1 cut(s) 109
Sse9I AATT 2 cut(s) 124, 254
SspMI CTAG 2 cut(s) 507, 640
StyI CCWWGG 1 cut(s) 28
TaaI ACNGT 1 cut(s) 45
TaiI ACGT 2 cut(s) 237, 557
TaqI TCGA 3 cut(s) 18, 237, 535
TasI AATT 2 cut(s) 124, 254
TfiI GAWTC 2 cut(s) 245, 361
TscAI CASTG 2 cut(s) 121, 517
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 4 cut(s) 13, 194, 485, 493
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 4 cut(s) 17, 117, 374, 554
TspRI CASTG 2 cut(s) 121, 517
VpaK11BI GGWCC 2 cut(s) 86, 629
XapI RAATTY 1 cut(s) 124
XceI RCATGY 2 cut(s) 109, 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 58
XspI CTAG 2 cut(s) 507, 640
ZraI GACGTC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.