Rroxscaffold_2G00150300

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008537
Physical Location & Seq
Reverse (-)
87704654 .. 87707068
2415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00150300.1

Sequence Viewer

Length: 747 bp
ATGAGTAGAGATAGTACAAGTTGGGCCTCGATAGGCATTGGACAAAGAAGCGAGCTGAGCTACCAAGGATTCTTGACTCTGGGTGCTTCGTCAGGGACACTCGAGATGAAGCTCAAGCAGCTTGAAAGCCTCCTTGGTGGTCTCCAACAGTTCTCCAACCCAAAGGTGGAGCTGGAACAATACCCAACTGGACCCCACATTGCATCTCGCATGCTCTACACTGCAGAGAATTCATTTGGGGATGTGTGTGACAAGGTAGTGGCTGATTTTGGCTGTGGTTGTGGTACATTGGGAGTTGCAGCTGGACTCTTGGGTGCAGAACATGTCATTGGCATTGACGTTGATCCAGAATCTCTTGAATTAGCTTCACTAAATGCTGAGGAACTTGAGTTGGACATAGATTATATTCAATGCAACATCAAGAACTTAGGGTGGAGAGCTGAAGTTGTTGATACTATTGTAATGAATCCTCCATTTGGAACTCGGAAAAAGGGTGCTGACATGGATTTTCTTTCTGTGGCTTTGAAGGTTGCTTCTCAAGCTGTTTATTCCTTACATAAAACCTCCACGAGAGATCATGTGAAAAGGGCAGCCTTGCAGCAGTTCAATGCTAGCAGTGCTGAGGTTATATGTGAGCTTCGATATGATTTACCACAAACGTACAAGTTTCATAAGAAACGAGAGGTGGATATCGCTGTGGACTTGTGGCGATTTGTCCCCAAGAGTAAGCAGGGTCCAGAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS PrmA

Protein Analysis

248

Amino Acids

27.22

Weight (kDa)

5.3

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PrmA PF06325 63 - 156 7.3e-11 Ribosomal protein L11 methyltransferase (PrmA)
MTS PF05175 83 - 164 5.4e-12 Methyltransferase small domain
Methyltransf_31 PF13847 85 - 159 6.7e-09 Methyltransferase domain
UPF0020 PF01170 85 - 164 3.3e-07 RMKL-like, methyltransferase domain
Methyltransf_25 PF13649 87 - 156 8.8e-10 Methyltransferase domain
Methyltransf_11 PF08241 89 - 156 3.5e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 338
AcsI RAATTY 1 cut(s) 229
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 3 cut(s) 16, 286, 662
AfiI CCNNNNNNNGG 2 cut(s) 166, 476
AflIII ACRYGT 1 cut(s) 322
AgsI TTSAA 5 cut(s) 125, 359, 410, 526, 607
AjuI GAANNNNNNNTTGG 4 cut(s) 117, 149, 312, 344
Alw26I GTCTC 1 cut(s) 146
AlwI GGATC 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 101
AoxI GGCC 1 cut(s) 24
ApeKI GCWGC 4 cut(s) 118, 299, 590, 598
ApoI RAATTY 1 cut(s) 229
AspS9I GGNCC 3 cut(s) 24, 191, 734
AsuNHI GCTAGC 1 cut(s) 611
AvaI CYCGRG 1 cut(s) 101
AvaII GGWCC 2 cut(s) 191, 734
BaeI ACNNNNGTAYC 2 cut(s) 444, 477
BauI CACGAG 1 cut(s) 568
BbvCI CCTCAGC 2 cut(s) 378, 621
BbvI GCAGC 4 cut(s) 130, 311, 602, 610
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 2 cut(s) 612, 745
BfmI CTRYAG 1 cut(s) 222
BglII AGATCT 1 cut(s) 741
BisI GCNGC 4 cut(s) 119, 300, 591, 599
BlpI GCTNAGC 1 cut(s) 56
BlsI GCNGC 4 cut(s) 120, 301, 592, 600
Bme18I GGWCC 2 cut(s) 191, 734
BmeT110I CYCGRG 1 cut(s) 101
BmgT120I GGNCC 3 cut(s) 24, 191, 734
BmiI GGNNCC 2 cut(s) 193, 735
BmsI GCATC 1 cut(s) 212
BmtI GCTAGC 1 cut(s) 615
Bpu10I CCTNAGC 2 cut(s) 378, 621
Bpu1102I GCTNAGC 1 cut(s) 56
BpuEI CTTGAG 3 cut(s) 98, 407, 522
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 64, 133
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 476
Bse1I ACTGG 1 cut(s) 193
Bse3DI GCAATG 1 cut(s) 198
BseDI CCNNGG 2 cut(s) 64, 133
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 2 cut(s) 166, 476
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 3 cut(s) 47, 369, 612
BseNI ACTGG 1 cut(s) 193
BseXI GCAGC 4 cut(s) 130, 311, 602, 610
BsgI GTGCAG 1 cut(s) 336
BshFI GGCC 1 cut(s) 26
BsiHKCI CYCGRG 1 cut(s) 101
BslFI GGGAC 2 cut(s) 109, 701
BslI CCNNNNNNNGG 2 cut(s) 166, 476
BsmAI GTCTC 1 cut(s) 146
BsmFI GGGAC 2 cut(s) 109, 701
BsnI GGCC 1 cut(s) 26
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 101
Bsp143I GATC 3 cut(s) 343, 574, 741
Bsp1720I GCTNAGC 1 cut(s) 56
BspANI GGCC 1 cut(s) 26
BspCNI CTCAG 3 cut(s) 48, 370, 613
BspLI GGNNCC 2 cut(s) 193, 735
BspMAI CTGCAG 1 cut(s) 226
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 198
BsrI ACTGG 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 64, 133
BssMI GATC 3 cut(s) 343, 574, 741
BssSI CACGAG 1 cut(s) 568
BssT1I CCWWGG 2 cut(s) 64, 133
Bst2BI CACGAG 1 cut(s) 568
Bst4CI ACNGT 1 cut(s) 150
BstC8I GCNNGC 3 cut(s) 53, 212, 613
BstDEI CTNAG 4 cut(s) 56, 378, 427, 621
BstF5I GGATG 1 cut(s) 247
BstKTI GATC 3 cut(s) 346, 577, 744
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 3 cut(s) 343, 574, 741
BstMWI GCNNNNNNNGC 4 cut(s) 57, 118, 539, 617
BstNSI RCATGY 2 cut(s) 214, 326
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 4 cut(s) 130, 311, 602, 610
BstX2I RGATCY 1 cut(s) 741
BstYI RGATCY 1 cut(s) 741
BsuRI GGCC 1 cut(s) 26
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 2 cut(s) 219, 622
BtsIMutI CAGTG 2 cut(s) 219, 622
Cac8I GCNNGC 3 cut(s) 53, 212, 613
Cfr13I GGNCC 3 cut(s) 24, 191, 734
Csp6I GTAC 3 cut(s) 15, 285, 661
CviAII CATG 4 cut(s) 211, 323, 502, 578
CviQI GTAC 3 cut(s) 15, 285, 661
DdeI CTNAG 4 cut(s) 56, 378, 427, 621
DpnI GATC 3 cut(s) 345, 576, 743
DpnII GATC 3 cut(s) 343, 574, 741
Eco130I CCWWGG 2 cut(s) 64, 133
Eco31I GGTCTC 1 cut(s) 146
Eco32I GATATC 1 cut(s) 691
Eco47I GGWCC 2 cut(s) 191, 734
Eco57I CTGAAG 1 cut(s) 462
Eco88I CYCGRG 1 cut(s) 101
EcoRI GAATTC 1 cut(s) 229
EcoRV GATATC 1 cut(s) 691
EcoT14I CCWWGG 2 cut(s) 64, 133
ErhI CCWWGG 2 cut(s) 64, 133
FaeI CATG 4 cut(s) 214, 326, 505, 581
FaqI GGGAC 2 cut(s) 109, 701
FatI CATG 4 cut(s) 210, 322, 501, 577
Fnu4HI GCNGC 4 cut(s) 119, 300, 591, 599
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 4 cut(s) 119, 300, 591, 599
FspBI CTAG 2 cut(s) 612, 745
GluI GCNGC 4 cut(s) 119, 300, 591, 599
HaeIII GGCC 1 cut(s) 26
Hin1II CATG 4 cut(s) 214, 326, 505, 581
HinfI GANTC 5 cut(s) 69, 76, 306, 350, 466
Hpy166II GTNNAC 1 cut(s) 700
Hpy188I TCNGA 1 cut(s) 486
Hpy188III TCNNGA 6 cut(s) 73, 103, 347, 356, 421, 737
Hpy8I GTNNAC 1 cut(s) 700
HpyAV CCTTC 1 cut(s) 520
HpyCH4III ACNGT 1 cut(s) 150
HpyCH4IV ACGT 2 cut(s) 339, 659
HpyCH4V TGCA 6 cut(s) 203, 224, 299, 317, 414, 598
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 118, 539, 617
HpyF3I CTNAG 4 cut(s) 56, 378, 427, 621
HpySE526I ACGT 2 cut(s) 339, 659
Hsp92II CATG 4 cut(s) 214, 326, 505, 581
Kzo9I GATC 3 cut(s) 343, 574, 741
LmnI GCTCC 1 cut(s) 169
LpnPI CCDG 7 cut(s) 65, 78, 158, 174, 288, 360, 716
Lsp1109I GCAGC 4 cut(s) 130, 311, 602, 610
LweI GCATC 1 cut(s) 212
MaeI CTAG 2 cut(s) 612, 745
MaeII ACGT 2 cut(s) 339, 659
MaeIII GTNAC 1 cut(s) 248
MalI GATC 3 cut(s) 345, 576, 743
MboI GATC 3 cut(s) 343, 574, 741
MflI RGATCY 1 cut(s) 741
MluCI AATT 2 cut(s) 229, 359
MlyI GAGTC 2 cut(s) 70, 300
MmeI TCCRAC 3 cut(s) 169, 180, 372
MnlI CCTC 7 cut(s) 37, 140, 373, 480, 574, 616, 676
MspA1I CMGCKG 1 cut(s) 302
MwoI GCNNNNNNNGC 4 cut(s) 57, 118, 539, 617
NdeII GATC 3 cut(s) 343, 574, 741
NheI GCTAGC 1 cut(s) 611
NlaIII CATG 4 cut(s) 214, 326, 505, 581
NlaIV GGNNCC 2 cut(s) 193, 735
NmuCI GTSAC 1 cut(s) 248
NspI RCATGY 2 cut(s) 214, 326
PaeI GCATGC 1 cut(s) 214
PaeR7I CTCGAG 1 cut(s) 101
PciI ACATGT 1 cut(s) 322
PfeI GAWTC 3 cut(s) 69, 350, 466
PkrI GCNGC 4 cut(s) 120, 301, 592, 600
PleI GAGTC 2 cut(s) 70, 300
PpsI GAGTC 2 cut(s) 70, 300
PscI ACATGT 1 cut(s) 322
PspN4I GGNNCC 2 cut(s) 193, 735
PspPI GGNCC 3 cut(s) 24, 191, 734
PstI CTGCAG 1 cut(s) 226
PsuI RGATCY 1 cut(s) 741
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 3 cut(s) 16, 286, 662
RsaNI GTAC 3 cut(s) 15, 285, 661
SatI GCNGC 4 cut(s) 119, 300, 591, 599
Sau3AI GATC 3 cut(s) 343, 574, 741
Sau96I GGNCC 3 cut(s) 24, 191, 734
SchI GAGTC 2 cut(s) 70, 300
SfaNI GCATC 1 cut(s) 212
SfcI CTRYAG 1 cut(s) 222
Sfr274I CTCGAG 1 cut(s) 101
SinI GGWCC 2 cut(s) 191, 734
SlaI CTCGAG 1 cut(s) 101
SmlI CTYRAG 4 cut(s) 101, 113, 386, 537
SmoI CTYRAG 4 cut(s) 101, 113, 386, 537
SphI GCATGC 1 cut(s) 214
Sse9I AATT 2 cut(s) 229, 359
SspMI CTAG 2 cut(s) 612, 745
StyI CCWWGG 2 cut(s) 64, 133
TaaI ACNGT 1 cut(s) 150
TaiI ACGT 2 cut(s) 342, 662
TaqI TCGA 3 cut(s) 29, 102, 640
TasI AATT 2 cut(s) 229, 359
TatI WGTACW 1 cut(s) 14
TfiI GAWTC 3 cut(s) 69, 350, 466
TscAI CASTG 2 cut(s) 226, 622
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 4 cut(s) 118, 299, 590, 598
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 4 cut(s) 122, 222, 479, 659
TspRI CASTG 2 cut(s) 226, 622
VpaK11BI GGWCC 2 cut(s) 191, 734
XapI RAATTY 1 cut(s) 229
XceI RCATGY 2 cut(s) 214, 326
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XhoI CTCGAG 1 cut(s) 101
XspI CTAG 2 cut(s) 612, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.