MD02G1060400.v1.1

Plant family of unknown function (DUF810)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011157
Physical Location & Seq
Forward (+)
4850912 .. 4855193
4282 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1060400.v1.1.491

Sequence Viewer

Length: 2943 bp
ATGGCTCAGATTCTCAGAGACAGAGTATTCGGAAGCTCCAGACGACACTCCCAAAATTCAATTCCAATTCCAATTCCAATCCAACCCGCCATGCCCTTTCACCCAGTTGAAGCCCTCCCCAACCCCTTTGGGGAACTGGGTCCGACCCTCTCGGACTCGGAGCTCCGGGAAACCGTCTACGAGATCTTAGTCGGAGCCTGCCGGAGCTCCGGGCCCAAGCCGCTCACCTACATCCCACAGTCCGAGAAGACCGACCGGAGTGCTCTGACTTCGCTCCCGTCGTCGCTTCAGAGGTCGACGTCGTCGGCGGCGAGCCGGGTCAAGAAGGCGCTGGGTTTGAAGTCGTCGTCTGGCAGGAGACTCGGTGGCGGTGACTCAGTGAGCCTGGGGAGGAGTAAGCGGACCGGGACGGTTTGGGAGCTCGTGAGAGTTCAGATGAAGGTTTCGGAGCAGACTGATACGAGGGTCCGGCGAGCACTTTTGAGGGTTGCTGCTGGTCAGCTTGGACGGCGAATAGAATGCATGGTATTGCCCCTTGAGCTATTACAGCAGTTCAAGTCTTCGGATTTTCCTAACCAACAAGAATATGAAGCTTGGCAGAGGAGAAACTTGAGGGTTCTCGAAGCCGGACTTCTTTTGTATCCTTATTTGCCTTTAGATAAGAGAGACACTGCTTCTCAACAACTCCGGAAGATTATTCATGGGGCCTTAGATAAACCCATAGAAACTGGAAAGCACACTGAGTCGATGCAAGTTCTTCGCAATGTTGTTATGTCTCTTGCTAACAGATCAGTTGACGGTTCTGTTTCCGAGACATGCCACTGGGCTGATGGGTTTCCGTTGAATCTGAGGCTCTACCAAATTCTACTAGAATCGTGTTTTGACCCTAATGAAGAAACATCTGTTATTGAAGAGCTTGATGAGGTTTTCGACCTCATAAAGAAGACCTGGGTGGTTCTCGGAATAAATCAAATGCTGCATAATCTTTGTTTCTCGTGGGTTTTATTTCATCGTTATGTTACAACTGGCCAAGTGGATAATGACCTGCTGCTTGCGTCCAGTAACCTGTTGGCGGAAGTTGAACAAGATGCCTATGGCACAAAAGATCCATCTTATTCAAAGATCTTAAGTTCGACATTGAGTTCGATATTGGGTTGGGCTGAGAAAAGGCTTCTTGCTTACCGAGATACTTTCCACAGTGGCAATATTGAAGCAATGCAAAACATTCTCTCTCTGGGGTTATTATCAGCAAAAATACTGGTTCAAGATATTTCTCATGAGTACCGTAGGAAGAGGAAAGAAACTAATGTGGGTTATGACAGGGTTGATGCTTACATAAGATCATCAATAAGCACCGCTTTTGCACAGAAACTGGAAAAGGTAGGTTCTAGCAAGCGCTACTCGAAGAGCCAAAACAATCTTCCTGCTCTTACCGTCCTTGCACAAGAAGTTAGTGAACTGGCTTTTAGTGAGAAGGAGATATTTGGTCCAGTATTCAAGAGATGGCACCCTCTTGCAACAGGCATTGCTATGGCTACGCTGCATTCTTGCTATGGAAATGAGCTGAAGCAATTTGTGGCGGGTCTCAGTGAGTTGACACCAGACACTATACAAGTGCTGAGAGCTGCTGACAAATTAGAAAAAGATCTTGTGCAGATTGCCGTCGAAGATTCAGTGGATAGTGAGGATGGGGGGAAATCGATCATAAGGGAGATGCCTCCTTACGAGACTGAAGCTGTAATTGCCAATCTGGCAAAGACTTGGATAAGGACAAGAGTAGACAGGCTGAGGGAATGGGTTGACAGAAATCTGCATCAAGAGGTATGGAATCCAAAGGCAAACAAAGAGCGGCTTGCTCCTTCTGCTGTTGAAGTTTTACGCATTATAGATGAAACGTTGGAAGCATTCTTTACGTTGCAGATACCCATGCATTCTGTCTTGGTTCCAGAACTGATGACCAGTCTTGACAAATGTATTCAACATTATGTCTTGAAGGCCAAATCTGGATGCGGGACCCTAAGCACTTCCATACCCGCTTTACCGGCTTTGACCAGATGCTCAGCAGGATCAAAATATGCTGTGTTCAAGAAAAAGGAAAGGTTGCATATAAGCCAGAGAAGGAAATCTCAGGTTGAAAGTACAAATGGGGACAGCTCATTTGGGATTCTGCAACTTTGTGTGCGCATTAATACTTTGCAGCTTATTCGAATGGAATTGGGAGTTTTTGAGAAGAGGATAATTGCCCATCTTGGGAGCTCAGAAACCCGTCAAGGGGACAATATAGCAAACGGAGTGGGCAAAATGTTTCAGCTTTCCGCATCTGCTTGTGCTGAAGGAATCCAACAACTTTGTGAGGCAACAGCATACAAGGTCGTTTTCCATGACCTAAGTCATGTCCTTTGGGACAGCTTGTATATTTTGGGAGTTTCGTCATCTAGGATTGAGCCATTTCTTCAGGAACTTGAGCAATACTTGGAGATTATTTCATCAACTGTGCACGACAGAGTTAGAACACGAGTCATTACTGATGTAATGAGAGCTTCTTTTGACGGGTTCTTGTTGGTTTTGCTAGCTGGAGGACCTTCTCGCACTTTCACGCAGAAAGATTCTGGCTTAATCGAGGAGGATTTTAAGTTTCTGACTGATTTATTCTGGTCAAACGGGGATGGACTGCCGGCTGATTTGATTGATAAGTTATCGACCACTGTTAAAAGTATCCTCCCTCTGTACGGTATTGATACTGACAGCCTTGTTGAACAGTTCAAACGTGCAACTCTGGAGAACTACGGATCTTCAGCTAAATCCCGGCTTCCAATGCCTCCAACTACAGGGGAATGGAATTCCAACGAGCCAAACACACTTCTACGTGTCTTGTGTTATCGGAATGACGAGACTGCAGCAAAGTTCCTTAAGAAGACTTACAACTTGCCTAAGAAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF810

Protein Analysis

981

Amino Acids

109.81

Weight (kDa)

8.45

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PATROL1 PF25761 581 - 979 7.2e-168 PATROL1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20010 AT2G20010
fragaria_vesca FvH4_1g05840
malus_domestica MD02G1060400.v1.1 MD15G1193900.v1.1
prunus_persica Prupe.7G222400_v2.0.a1 Prupe.7G222400_v2.0.a1
pyrus_communis pycom02g04960 pycom15g17370
rosa_chinensis RchiOBHm_Chr2g0091501 RchiOBHm_Chr2g0091591
rosa_laevigata RLG00000016231
rosa_multiflora Rmu_sc0002407.1_g000007
rosa_roxburghii Rroxscaffold_2G00149980
rosa_rugosa Rorug02G0018300
rosa_samantha Rh2AG063500 Rh2BG062900 Rh2CG064600 Rh2DG063000
rosa_wichuraiana Rw2G005690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 302
Acc16I TGCGCA 1 cut(s) 2183
Acc36I ACCTGC 1 cut(s) 1053
AccB1I GGYRCC 1 cut(s) 1506
AccBSI CCGCTC 2 cut(s) 223, 1849
AccI GTMKAC 3 cut(s) 177, 296, 1779
AccIII TCCGGA 1 cut(s) 687
AclI AACGTT 1 cut(s) 1895
AclWI GGATC 3 cut(s) 1100, 2074, 2797
AcoI YGGCCR 1 cut(s) 1027
AcsI RAATTY 3 cut(s) 55, 861, 2839
AcuI CTGAAG 6 cut(s) 272, 1586, 1752, 2352, 2438, 2778
AcyI GRCGYC 1 cut(s) 299
AfaI GTAC 3 cut(s) 1283, 2140, 2729
AfeI AGCGCT 1 cut(s) 1397
AfiI CCNNNNNNNGG 7 cut(s) 130, 1072, 2250, 2271, 2272, 2729, 2828
AflII CTTAAG 2 cut(s) 1126, 2909
AflIII ACRYGT 1 cut(s) 2866
AjnI CCWGG 2 cut(s) 384, 947
AjuI GAANNNNNNNTTGG 4 cut(s) 45, 77, 1404, 1436
Alw21I GWGCWC 7 cut(s) 165, 209, 265, 423, 478, 2258, 2499
Alw26I GTCTC 8 cut(s) 12, 352, 660, 780, 806, 1589, 1721, 2885
Alw44I GTGCAC 1 cut(s) 2495
AlwI GGATC 3 cut(s) 1100, 2074, 2797
AlwNI CAGNNNCTG 1 cut(s) 1372
Aor13HI TCCGGA 1 cut(s) 687
Aor51HI AGCGCT 1 cut(s) 1397
AoxI GGCC 4 cut(s) 212, 705, 1027, 1995
ApaI GGGCCC 1 cut(s) 216
ApaLI GTGCAC 1 cut(s) 2495
ApeKI GCWGC 7 cut(s) 491, 976, 1048, 1540, 1625, 2197, 2897
ApoI RAATTY 3 cut(s) 55, 861, 2839
ArsI GACNNNNNNTTYG 6 cut(s) 11, 43, 1126, 1158, 2141, 2173
AseI ATTAAT 1 cut(s) 2187
AspLEI GCGC 3 cut(s) 331, 1398, 2184
AspS9I GGNCC 9 cut(s) 140, 212, 213, 402, 466, 705, 1487, 2013, 2579
AsuC2I CCSGG 5 cut(s) 167, 211, 317, 406, 2806
AsuHPI GGTGA 3 cut(s) 92, 217, 383
AsuII TTCGAA 1 cut(s) 2206
AsuNHI GCTAGC 1 cut(s) 2569
AvaII GGWCC 6 cut(s) 140, 402, 466, 1487, 2013, 2579
BaeGI GKGCMC 2 cut(s) 216, 2499
BaeI ACNNNNGTAYC 2 cut(s) 450, 483
BalI TGGCCA 1 cut(s) 1029
BanI GGYRCC 1 cut(s) 1506
BanII GRGCYC 5 cut(s) 165, 209, 216, 423, 2258
BauI CACGAG 3 cut(s) 422, 994, 2514
BbsI GAAGAC 4 cut(s) 254, 552, 950, 2921
Bbv12I GWGCWC 7 cut(s) 165, 209, 265, 423, 478, 2258, 2499
BbvCI CCTCAGC 1 cut(s) 1787
BbvI GCAGC 7 cut(s) 478, 963, 1035, 1527, 1612, 2209, 2909
BccI CCATC 6 cut(s) 824, 1117, 1497, 1682, 2253, 2660
BceAI ACGGC 2 cut(s) 524, 1646
BciT130I CCWGG 2 cut(s) 386, 949
BciVI GTATCC 2 cut(s) 651, 2726
BcnI CCSGG 5 cut(s) 167, 211, 317, 406, 2806
BcoDI GTCTC 8 cut(s) 12, 352, 660, 780, 806, 1589, 1721, 2885
BfaI CTAG 4 cut(s) 869, 1389, 2436, 2570
BfmI CTRYAG 2 cut(s) 2826, 2895
BfoI RGCGCY 2 cut(s) 332, 1399
BfrI CTTAAG 2 cut(s) 1126, 2909
BfuAI ACCTGC 1 cut(s) 1053
BfuI GTATCC 2 cut(s) 651, 2726
BglI GCCNNNNNGGC 1 cut(s) 1751
BglII AGATCT 3 cut(s) 183, 1122, 1645
BlpI GCTNAGC 1 cut(s) 2059
Bme1390I CCNGG 7 cut(s) 167, 211, 317, 386, 406, 949, 2806
Bme18I GGWCC 6 cut(s) 140, 402, 466, 1487, 2013, 2579
BmgT120I GGNCC 9 cut(s) 140, 212, 213, 402, 466, 705, 1487, 2013, 2579
BmiI GGNNCC 9 cut(s) 141, 196, 214, 467, 706, 1508, 1944, 2014, 2015
BmrFI CCNGG 7 cut(s) 167, 211, 317, 386, 406, 949, 2806
BmrI ACTGGG 3 cut(s) 98, 146, 832
BmsI GCATC 8 cut(s) 738, 1078, 1318, 1704, 1822, 1997, 2045, 2327
BmtI GCTAGC 1 cut(s) 2573
BmuI ACTGGG 3 cut(s) 98, 146, 832
BoxI GACNNNNGTC 1 cut(s) 2388
BpiI GAAGAC 4 cut(s) 254, 552, 950, 2921
BpmI CTGGAG 3 cut(s) 22, 2595, 2798
Bpu10I CCTNAGC 2 cut(s) 1787, 2018
Bpu1102I GCTNAGC 1 cut(s) 2059
Bpu14I TTCGAA 1 cut(s) 2206
BpuEI CTTGAG 3 cut(s) 557, 631, 2483
BpuMI CCSGG 5 cut(s) 167, 211, 317, 406, 2806
Bsa29I ATCGAT 1 cut(s) 1700
BsaAI YACGTR 1 cut(s) 2867
BsaHI GRCGYC 1 cut(s) 299
BsaI GGTCTC 1 cut(s) 1589
BsaJI CCNNGG 2 cut(s) 385, 948
BsaWI WCCGGW 2 cut(s) 255, 687
BsaXI ACNNNNNCTCC 2 cut(s) 349, 379
Bsc4I CCNNNNNNNGG 7 cut(s) 130, 1072, 2250, 2271, 2272, 2729, 2828
Bse118I RCCGGY 2 cut(s) 2041, 2674
Bse3DI GCAATG 3 cut(s) 769, 1221, 1524
BseAI TCCGGA 1 cut(s) 687
BseBI CCWGG 2 cut(s) 386, 949
BseCI ATCGAT 1 cut(s) 1700
BseDI CCNNGG 2 cut(s) 385, 948
BseGI GGATG 4 cut(s) 231, 1693, 2012, 2671
BseLI CCNNNNNNNGG 7 cut(s) 130, 1072, 2250, 2271, 2272, 2729, 2828
BseMI GCAATG 3 cut(s) 769, 1221, 1524
BseRI GAGGAG 3 cut(s) 406, 616, 2636
BseSI GKGCMC 2 cut(s) 216, 2499
BseXI GCAGC 7 cut(s) 478, 963, 1035, 1527, 1612, 2209, 2909
BseYI CCCAGC 1 cut(s) 331
BsgI GTGCAG 1 cut(s) 1673
Bsh1285I CGRYCG 1 cut(s) 256
BshFI GGCC 4 cut(s) 214, 707, 1029, 1997
BshNI GGYRCC 1 cut(s) 1506
BshVI ATCGAT 1 cut(s) 1700
BsiEI CGRYCG 1 cut(s) 256
BsiHKAI GWGCWC 7 cut(s) 165, 209, 265, 423, 478, 2258, 2499
BslFI GGGAC 5 cut(s) 421, 2026, 2162, 2288, 2417
BslI CCNNNNNNNGG 7 cut(s) 130, 1072, 2250, 2271, 2272, 2729, 2828
BsmAI GTCTC 8 cut(s) 12, 352, 660, 780, 806, 1589, 1721, 2885
BsmFI GGGAC 5 cut(s) 421, 2026, 2162, 2288, 2417
BsmI GAATGC 4 cut(s) 524, 1543, 1904, 1930
BsnI GGCC 4 cut(s) 214, 707, 1029, 1997
Bso31I GGTCTC 1 cut(s) 1589
Bsp119I TTCGAA 1 cut(s) 2206
Bsp120I GGGCCC 1 cut(s) 212
Bsp1286I GDGCHC 8 cut(s) 165, 209, 216, 265, 423, 478, 2258, 2499
Bsp13I TCCGGA 1 cut(s) 687
Bsp143I GATC 9 cut(s) 183, 788, 1105, 1122, 1340, 1645, 1701, 2066, 2789
Bsp1720I GCTNAGC 1 cut(s) 2059
BspANI GGCC 4 cut(s) 214, 707, 1029, 1997
BspDI ATCGAT 1 cut(s) 1700
BspEI TCCGGA 1 cut(s) 687
BspHI TCATGA 1 cut(s) 1276
BspLI GGNNCC 9 cut(s) 141, 196, 214, 467, 706, 1508, 1944, 2014, 2015
BspMAI CTGCAG 1 cut(s) 2899
BspMI ACCTGC 1 cut(s) 1053
BspOI GCTAGC 1 cut(s) 2573
BspPI GGATC 3 cut(s) 1100, 2074, 2797
BspQI GCTCTTC 2 cut(s) 906, 1400
BspT104I TTCGAA 1 cut(s) 2206
BspT107I GGYRCC 1 cut(s) 1506
BspTI CTTAAG 2 cut(s) 1126, 2909
BspTNI GGTCTC 1 cut(s) 1589
BsrBI CCGCTC 2 cut(s) 223, 1849
BsrDI GCAATG 3 cut(s) 769, 1221, 1524
BsrFI RCCGGY 2 cut(s) 2041, 2674
BssAI RCCGGY 2 cut(s) 2041, 2674
BssECI CCNNGG 2 cut(s) 385, 948
BssMI GATC 9 cut(s) 183, 788, 1105, 1122, 1340, 1645, 1701, 2066, 2789
BssNI GRCGYC 1 cut(s) 299
BssSI CACGAG 3 cut(s) 422, 994, 2514
Bst2BI CACGAG 3 cut(s) 422, 994, 2514
Bst2UI CCWGG 2 cut(s) 386, 949
Bst6I CTCTTC 4 cut(s) 906, 1286, 1400, 2225
BstACI GRCGYC 1 cut(s) 299
BstAFI CTTAAG 2 cut(s) 1126, 2909
BstBAI YACGTR 1 cut(s) 2867
BstBI TTCGAA 1 cut(s) 2206
BstC8I GCNNGC 8 cut(s) 199, 313, 474, 1053, 1394, 1854, 2571, 2676
BstF5I GGATG 4 cut(s) 231, 1693, 2012, 2671
BstH2I RGCGCY 2 cut(s) 332, 1399
BstHHI GCGC 3 cut(s) 331, 1398, 2184
BstKTI GATC 9 cut(s) 186, 791, 1108, 1125, 1343, 1648, 1704, 2069, 2792
BstMAI GTCTC 8 cut(s) 12, 352, 660, 780, 806, 1589, 1721, 2885
BstMBI GATC 9 cut(s) 183, 788, 1105, 1122, 1340, 1645, 1701, 2066, 2789
BstMCI CGRYCG 1 cut(s) 256
BstNI CCWGG 2 cut(s) 386, 949
BstNSI RCATGY 1 cut(s) 819
BstPAI GACNNNNGTC 1 cut(s) 2388
BstSCI CCNGG 7 cut(s) 165, 209, 315, 384, 404, 947, 2804
BstSFI CTRYAG 2 cut(s) 2826, 2895
BstSLI GKGCMC 2 cut(s) 216, 2499
BstV1I GCAGC 7 cut(s) 478, 963, 1035, 1527, 1612, 2209, 2909
BstV2I GAAGAC 4 cut(s) 254, 552, 950, 2921
BstX2I RGATCY 5 cut(s) 183, 1105, 1122, 1645, 2789
BstYI RGATCY 5 cut(s) 183, 1105, 1122, 1645, 2789
Bsu15I ATCGAT 1 cut(s) 1700
BsuI GTATCC 2 cut(s) 651, 2726
BsuRI GGCC 4 cut(s) 214, 707, 1029, 1997
BsuTUI ATCGAT 1 cut(s) 1700
BtsCI GGATG 4 cut(s) 231, 1693, 2012, 2671
BtsI GCAGTG 1 cut(s) 669
BtsIMutI CAGTG 8 cut(s) 384, 669, 738, 820, 1204, 1594, 1680, 2703
BveI ACCTGC 1 cut(s) 1053
Cac8I GCNNGC 8 cut(s) 199, 313, 474, 1053, 1394, 1854, 2571, 2676
CaiI CAGNNNCTG 1 cut(s) 1372
CciI TCATGA 1 cut(s) 1276
CfoI GCGC 3 cut(s) 331, 1398, 2184
Cfr10I RCCGGY 2 cut(s) 2041, 2674
Cfr13I GGNCC 9 cut(s) 140, 212, 213, 402, 466, 705, 1487, 2013, 2579
ClaI ATCGAT 1 cut(s) 1700
CpoI CGGWCCG 1 cut(s) 402
CseI GACGC 1 cut(s) 1044
Csp6I GTAC 3 cut(s) 1282, 2139, 2728
CspCI CAANNNNNGTGG 2 cut(s) 2274, 2309
CspI CGGWCCG 1 cut(s) 402
CviAII CATG 8 cut(s) 91, 523, 701, 816, 1277, 1927, 2381, 2393
CviQI GTAC 3 cut(s) 1282, 2139, 2728
DpnI GATC 9 cut(s) 185, 790, 1107, 1124, 1342, 1647, 1703, 2068, 2791
DpnII GATC 9 cut(s) 183, 788, 1105, 1122, 1340, 1645, 1701, 2066, 2789
EaeI YGGCCR 1 cut(s) 1027
Eam1104I CTCTTC 4 cut(s) 906, 1286, 1400, 2225
EarI CTCTTC 4 cut(s) 906, 1286, 1400, 2225
EciI GGCGGA 1 cut(s) 1088
Ecl136II GAGCTC 4 cut(s) 163, 207, 421, 2256
Eco24I GRGCYC 5 cut(s) 165, 209, 216, 423, 2258
Eco31I GGTCTC 1 cut(s) 1589
Eco47I GGWCC 6 cut(s) 140, 402, 466, 1487, 2013, 2579
Eco47III AGCGCT 1 cut(s) 1397
Eco53kI GAGCTC 4 cut(s) 163, 207, 421, 2256
Eco57I CTGAAG 6 cut(s) 272, 1586, 1752, 2352, 2438, 2778
EcoICRI GAGCTC 4 cut(s) 163, 207, 421, 2256
EcoO109I RGGNCCY 3 cut(s) 705, 2013, 2579
EcoRI GAATTC 1 cut(s) 2839
EcoRII CCWGG 2 cut(s) 384, 947
EcoT22I ATGCAT 2 cut(s) 524, 1932
EcoT38I GRGCYC 5 cut(s) 165, 209, 216, 423, 2258
FaeI CATG 8 cut(s) 94, 526, 704, 819, 1280, 1930, 2384, 2396
FalI AAGNNNNNCTT 6 cut(s) 615, 647, 1342, 1374, 1836, 1868
FaqI GGGAC 5 cut(s) 421, 2026, 2162, 2288, 2417
FatI CATG 8 cut(s) 90, 522, 700, 815, 1276, 1926, 2380, 2392
FauI CCCGC 4 cut(s) 94, 1573, 2003, 2041
FblI GTMKAC 3 cut(s) 177, 296, 1779
FokI GGATG 4 cut(s) 218, 1700, 2019, 2678
FriOI GRGCYC 5 cut(s) 165, 209, 216, 423, 2258
FspAI RTGCGCAY 1 cut(s) 2183
FspBI CTAG 4 cut(s) 869, 1389, 2436, 2570
FspI TGCGCA 1 cut(s) 2183
GlaI GCGC 3 cut(s) 330, 1397, 2183
GsaI CCCAGC 1 cut(s) 335
GsuI CTGGAG 3 cut(s) 22, 2595, 2798
HaeII RGCGCY 2 cut(s) 332, 1399
HaeIII GGCC 4 cut(s) 214, 707, 1029, 1997
HgaI GACGC 1 cut(s) 1044
HhaI GCGC 3 cut(s) 331, 1398, 2184
Hin1I GRCGYC 1 cut(s) 299
Hin1II CATG 8 cut(s) 94, 526, 704, 819, 1280, 1930, 2384, 2396
Hin6I GCGC 3 cut(s) 329, 1396, 2182
HinP1I GCGC 3 cut(s) 329, 1396, 2182
HincII GTYRAC 4 cut(s) 297, 796, 1596, 1801
HindII GTYRAC 4 cut(s) 297, 796, 1596, 1801
HindIII AAGCTT 1 cut(s) 591
HphI GGTGA 3 cut(s) 92, 217, 383
Hpy166II GTNNAC 8 cut(s) 178, 297, 796, 1457, 1596, 1780, 1801, 2497
Hpy8I GTNNAC 8 cut(s) 178, 297, 796, 1457, 1596, 1780, 1801, 2497
Hpy99I CGWCG 7 cut(s) 283, 286, 301, 304, 307, 349, 1667
HpyAV CCTTC 8 cut(s) 319, 433, 1468, 1869, 1987, 2112, 2327, 2592
HpyCH4IV ACGT 5 cut(s) 299, 1895, 1913, 2767, 2866
HpySE526I ACGT 5 cut(s) 299, 1895, 1913, 2767, 2866
Hsp92I GRCGYC 1 cut(s) 299
Hsp92II CATG 8 cut(s) 94, 526, 704, 819, 1280, 1930, 2384, 2396
HspAI GCGC 3 cut(s) 329, 1396, 2182
KflI GGGWCCC 1 cut(s) 2013
Kpn2I TCCGGA 1 cut(s) 687
KroI GCCGGC 1 cut(s) 2674
KroNI GCCGGC 1 cut(s) 2676
Kzo9I GATC 9 cut(s) 183, 788, 1105, 1122, 1340, 1645, 1701, 2066, 2789
LguI GCTCTTC 2 cut(s) 906, 1400
Lsp1109I GCAGC 7 cut(s) 478, 963, 1035, 1527, 1612, 2209, 2909
LweI GCATC 8 cut(s) 738, 1078, 1318, 1704, 1822, 1997, 2045, 2327
MaeI CTAG 4 cut(s) 869, 1389, 2436, 2570
MaeII ACGT 5 cut(s) 299, 1895, 1913, 2767, 2866
MaeIII GTNAC 3 cut(s) 371, 1018, 1061
MalI GATC 9 cut(s) 185, 790, 1107, 1124, 1342, 1647, 1703, 2068, 2791
MbiI CCGCTC 2 cut(s) 223, 1849
MboI GATC 9 cut(s) 183, 788, 1105, 1122, 1340, 1645, 1701, 2066, 2789
MflI RGATCY 5 cut(s) 183, 1105, 1122, 1645, 2789
MhlI GDGCHC 8 cut(s) 165, 209, 216, 265, 423, 478, 2258, 2499
MlsI TGGCCA 1 cut(s) 1029
MluNI TGGCCA 1 cut(s) 1029
MlyI GAGTC 5 cut(s) 149, 354, 368, 752, 2526
MmeI TCCRAC 7 cut(s) 106, 167, 172, 1878, 2365, 2846, 2868
Mox20I TGGCCA 1 cut(s) 1029
Mph1103I ATGCAT 2 cut(s) 524, 1932
MroI TCCGGA 1 cut(s) 687
MroNI GCCGGC 1 cut(s) 2674
MscI TGGCCA 1 cut(s) 1029
MseI TTAA 6 cut(s) 1127, 2187, 2615, 2631, 2709, 2910
MslI CAYNNNNRTG 2 cut(s) 1014, 1529
Msp20I TGGCCA 1 cut(s) 1029
MspCI CTTAAG 2 cut(s) 1126, 2909
MspR9I CCNGG 7 cut(s) 167, 211, 317, 386, 406, 949, 2806
Mva1269I GAATGC 4 cut(s) 524, 1543, 1904, 1930
MvaI CCWGG 2 cut(s) 386, 949
NaeI GCCGGC 1 cut(s) 2676
NciI CCSGG 5 cut(s) 167, 211, 317, 406, 2806
NdeII GATC 9 cut(s) 183, 788, 1105, 1122, 1340, 1645, 1701, 2066, 2789
NgoMIV GCCGGC 1 cut(s) 2674
NheI GCTAGC 1 cut(s) 2569
NlaIII CATG 8 cut(s) 94, 526, 704, 819, 1280, 1930, 2384, 2396
NlaIV GGNNCC 9 cut(s) 141, 196, 214, 467, 706, 1508, 1944, 2014, 2015
NmuCI GTSAC 1 cut(s) 371
NsbI TGCGCA 1 cut(s) 2183
NsiI ATGCAT 2 cut(s) 524, 1932
NspI RCATGY 1 cut(s) 819
NspV TTCGAA 1 cut(s) 2206
PagI TCATGA 1 cut(s) 1276
PciSI GCTCTTC 2 cut(s) 906, 1400
PcsI WCGNNNNNNNCGW 2 cut(s) 278, 308
PctI GAATGC 4 cut(s) 524, 1543, 1904, 1930
PdiI GCCGGC 1 cut(s) 2676
PfeI GAWTC 8 cut(s) 10, 844, 872, 1670, 1828, 2164, 2337, 2606
PflFI GACNNNGTC 1 cut(s) 301
PfoI TCCNGGA 1 cut(s) 165
PleI GAGTC 5 cut(s) 149, 354, 368, 751, 2525
PpsI GAGTC 5 cut(s) 149, 354, 368, 751, 2525
Ppu21I YACGTR 1 cut(s) 2867
PpuMI RGGWCCY 2 cut(s) 2013, 2579
PshAI GACNNNNGTC 1 cut(s) 2388
PshBI ATTAAT 1 cut(s) 2187
Psp124BI GAGCTC 4 cut(s) 165, 209, 423, 2258
Psp1406I AACGTT 1 cut(s) 1895
Psp5II RGGWCCY 2 cut(s) 2013, 2579
Psp6I CCWGG 2 cut(s) 384, 947
PspFI CCCAGC 1 cut(s) 331
PspGI CCWGG 2 cut(s) 384, 947
PspN4I GGNNCC 9 cut(s) 141, 196, 214, 467, 706, 1508, 1944, 2014, 2015
PspOMI GGGCCC 1 cut(s) 212
PspPI GGNCC 9 cut(s) 140, 212, 213, 402, 466, 705, 1487, 2013, 2579
PspPPI RGGWCCY 2 cut(s) 2013, 2579
PstI CTGCAG 1 cut(s) 2899
PstNI CAGNNNCTG 1 cut(s) 1372
PsuI RGATCY 5 cut(s) 183, 1105, 1122, 1645, 2789
PsyI GACNNNGTC 1 cut(s) 301
RsaI GTAC 3 cut(s) 1283, 2140, 2729
RsaNI GTAC 3 cut(s) 1282, 2139, 2728
RseI CAYNNNNRTG 2 cut(s) 1014, 1529
Rsr2I CGGWCCG 1 cut(s) 402
RsrII CGGWCCG 1 cut(s) 402
SacI GAGCTC 4 cut(s) 165, 209, 423, 2258
SalI GTCGAC 1 cut(s) 295
SapI GCTCTTC 2 cut(s) 906, 1400
SaqAI TTAA 6 cut(s) 1127, 2187, 2615, 2631, 2709, 2910
Sau3AI GATC 9 cut(s) 183, 788, 1105, 1122, 1340, 1645, 1701, 2066, 2789
Sau96I GGNCC 9 cut(s) 140, 212, 213, 402, 466, 705, 1487, 2013, 2579
SchI GAGTC 5 cut(s) 149, 354, 368, 752, 2526
ScrFI CCNGG 7 cut(s) 167, 211, 317, 386, 406, 949, 2806
SduI GDGCHC 8 cut(s) 165, 209, 216, 265, 423, 478, 2258, 2499
SfaNI GCATC 8 cut(s) 738, 1078, 1318, 1704, 1822, 1997, 2045, 2327
SfcI CTRYAG 2 cut(s) 2826, 2895
SfuI TTCGAA 1 cut(s) 2206
SinI GGWCC 6 cut(s) 140, 402, 466, 1487, 2013, 2579
SmiMI CAYNNNNRTG 2 cut(s) 1014, 1529
SmlI CTYRAG 5 cut(s) 536, 610, 1126, 2462, 2909
SmoI CTYRAG 5 cut(s) 536, 610, 1126, 2462, 2909
SspI AATATT 1 cut(s) 1207
SspMI CTAG 4 cut(s) 869, 1389, 2436, 2570
SstI GAGCTC 4 cut(s) 165, 209, 423, 2258
StyD4I CCNGG 7 cut(s) 165, 209, 315, 384, 404, 947, 2804
TaiI ACGT 5 cut(s) 302, 1898, 1916, 2770, 2869
TaqII GACCGA 1 cut(s) 266
TatI WGTACW 1 cut(s) 2138
TauI GCSGC 3 cut(s) 223, 311, 1852
TfiI GAWTC 8 cut(s) 10, 844, 872, 1670, 1828, 2164, 2337, 2606
Tru1I TTAA 6 cut(s) 1127, 2187, 2615, 2631, 2709, 2910
Tru9I TTAA 6 cut(s) 1127, 2187, 2615, 2631, 2709, 2910
TscAI CASTG 8 cut(s) 384, 676, 745, 827, 1204, 1594, 1680, 2710
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 7 cut(s) 491, 976, 1048, 1540, 1625, 2197, 2897
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 7 cut(s) 452, 603, 689, 906, 998, 1905, 2475
TspGWI ACGGA 3 cut(s) 828, 2304, 2802
TspRI CASTG 8 cut(s) 384, 676, 745, 827, 1204, 1594, 1680, 2710
Tth111I GACNNNGTC 1 cut(s) 301
Vha464I CTTAAG 2 cut(s) 1126, 2909
VneI GTGCAC 1 cut(s) 2495
VpaK11BI GGWCC 6 cut(s) 140, 402, 466, 1487, 2013, 2579
VspI ATTAAT 1 cut(s) 2187
XapI RAATTY 3 cut(s) 55, 861, 2839
XceI RCATGY 1 cut(s) 819
XcmI CCANNNNNNNNNTGG 2 cut(s) 827, 1066
XmiI GTMKAC 3 cut(s) 177, 296, 1779
XspI CTAG 4 cut(s) 869, 1389, 2436, 2570
ZraI GACGTC 1 cut(s) 300
Zsp2I ATGCAT 2 cut(s) 524, 1932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.