RLG00000016231

Plant family of unknown function (DUF810)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011157
Physical Location & Seq
Forward (+)
4916278 .. 4920635
4358 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016231

Sequence Viewer

Length: 2958 bp
ATGGCCCAGATTATCAGAGACAGAGTTTTCGGCTCCTTTGGTGGTTCCAGACGACACAACAGAGTCCCTATTCCAATTCCAGAACCCGAACCCGGACCCAAGCGCGTCGTCATGCCCGTTTACCCGGTCGAAGACCTGCCCAACCCGTTTGGGGACTTGGGCCCGCATCTCTCCGACTCGGACCTCCGAGAAACCGTCTACGAGATCCTAGTCGGAGCCTGCCGGAGCTCAGGCCCGAAACCGCTCACCTACACCCCGCAGTCGGAGAAGATCACCGACCGGAATTCCCAGTCTTCTTCTTCTTCCTCTTCCTCGCTGTCGTCACTTCAGAGGTCGCTGACGTCATCGGCGAAGAAGGCTCTGAGTTTGAAGCAAACGGCGTCGTCTAAGAGGCTCGGAGGGAGTAGTAAACGGTCCGGGTCGGTTTTTGAGCTCATGCGGGTCCAGATGAGGGTTTCGGAGCAGACCGATACCCGAATCAGGAGAGCCCTTCTCAGAGTTGCTGCTGGTCAGCTCGGACGAAAGATAGAGAGCATGGTTTTGCCTCTGGAGCTGTTACAGCATTTCAGGTCGTTAGATTTTGCGAATCAGCAGGAGTATGAAGCTTGGCAGAGGAGGAATTTGAAGGTTCTTGAAGTGGGACTTCTGGTGTATCCTCATCTGCCGTCAGATAGAAAAGACACCGCTCCTCAACAGCTCCGGAAGATTATACGTGGGGCCTTGGAGAAACCCATGGAAACTGGAAAGAACACCGAGACAATGCAAGTTCTTCGCAGTCTTGTTATGTCACTTGCCTGCAGGTCGTTTGATGGTACTGTTTCCGACACATGCCACTGGGCAGATGGGTTTCCGTTGAACCTCAGGCTCTACCAAAAGCTCCTGGAATCGTGTTTTGACCTGAATGAAGAAACCTCTATTATTGAAGAGCTTGATGAGGTTTTAGAAGTCATGAAGAAGACCTGGGTAGTCCTTGGAATAAACCAAATTCTGCATAATCTGTGTTTTGCATGGGTTTTATTTCATCGATATGTTACAACTGGCCAAGTGGATAATGACCTGCTGTTTGCAGCCAATAACCTCTTGGCGGAAGTTCAGCAAGATGCCAATGCCACAAAAGATCCATCTTATTTGAAGATCGTAAGTTCAACATTGAATGCAATACTGGGTTGGACTGAGAAAAGGCTTCTTGCCTACCGTGATGTTTTCAACACTGGCAATATTGAGTCGATGCAAAACACAGTCTCCCTGGGGGTATTATCAGCAAAAGTACTAGTAGAGGATATCTCTCACGAGTATCGTAGGAAGAGAAAAGAAGTTGATGTGGCACGTGACAGGGTTGACACCTACATAAGATCGTCAATACGCACCGCTTTTGCTCAGAAACTGGAAAAAGTGGGCAACAAGCGCCTATCAAAGAGCCAAAAGAATCGTATTCCTACCTTGTCCGTCCTTGCAACAGAAGTTAGCGAACTGGCCTTTACTGAGAAAGATATATTTGGTCCAGTGTTGAAGACATGGCACCCTTTTGCAACGGGTGTTGCTATGGCTACGCTGCATTCTTGCTATGGAGACGAGCTCAAGCAATTTGTGGATGGTATCAGTGAGTTGACTCCTGATACTGTGCAAATACTAAAAGCTGCTGACAAATTGGAGAAAGATCTTGTGCAGATTGCTGTCGAAGATTCAGTAGATAGTGAGGATGGCGGGAAATCCATCATACAGGAGATGCCTCCTTACGAGGCTGAAGCTGTAATGGCTGAGCTCGCAAAGGCTTGGATAAGGACAAGATTGGACAGGCTGAAGGAATGGGTTGACAGAAATCTGCAACAAGAGGTCTGGAATCCACAAGCGAACAAAGAACGCTTTGCTCCTTCTGCCATTGAAGTTTTACGCATTGTAGATGAAACATTGGAAGCATTCTTTATGTTGCCTATACCAATGCATCCTGGCTTGATTCCAGAATTGAAGACTGGTCTTGATAGATGTCTTCAATACTATGTATCGAAGGCCAAGACTGGCTGTGGAACTCGAATTACCTTCATTCCCACTATGCCTGCTTTGACTAGATGTTCAGCAGGATCAAAATTTCATGGTGTATTCAAGAAGAAGGAAAGGTCACATATAAACCAGAGGAGGAAATCTCAGGTTGGAAGTACAAATGGGGATGGTTCGTTTCGGATTCCGCAACTTTGTGTTCGCATTAATACTCTGCAGCGTATTCGAATGGAGTTGGGAGTTTTTGAGAAGAGGATTGTCGCTCATCTTGGGAACTTGGAATCCACTCAAAAGGGAGATTTTGTGAGTGGGATGGGCAAAATGTTTGAGCTCTCAGCATCTGCTTGTTTAGAAGGGATCCAACAACTTTGTGAGGCAACAGCTTACAAGGTTGTTTTCCATGAACTAAGTCATGTTCTTTGGGATGGCTTGTATAATGTGGATGCTCCCTCATGTAGAATTGAGCCGTTTCTTCAGGAACTCGAGCAATTTTTGGAGATTATCTCGTCAACAGTTCACGACAGAGTTAGAACGCGTCTTATTACGGATGTCATGAAAGCTTCTTTTGACGGGTTCTTGTTGGTTTTGCTAGCTGGAGGCCCTTCCCGCAGTTTCACACTGAGAGATTCTAACATAATAGAGGAGGATTTTAAATTTCTGACTGATTTGTTCTGGTCAAACGGGGATGGATTACCAGCTGATTTAATAGCTAAGTTGTCAACCACTGTTAAGGACATCCTTCCTCTCTACCGTACTGATACTGATAGCCTTATTGAAAAGTTCAAGCGCGCGACTCTGGAGAACTATGGCTCTTCTGCGAAGTCCCACCTGCCATTGCCTCCAACTTCCGATGAGTGGAATTCCAATGACCCAAATACACTTCTACGTGTCTTATGTCATAGGAATGATGAGACTGCAGCCAAGTTTCTGAAGAAGACTTACAATTTGCCTACAAAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF810

Protein Analysis

986

Amino Acids

110.92

Weight (kDa)

8.43

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PATROL1 PF25761 586 - 984 5.4e-167 PATROL1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20010 AT2G20010
fragaria_vesca FvH4_1g05840
malus_domestica MD02G1060400.v1.1 MD15G1193900.v1.1
prunus_persica Prupe.7G222400_v2.0.a1 Prupe.7G222400_v2.0.a1
pyrus_communis pycom02g04960 pycom15g17370
rosa_chinensis RchiOBHm_Chr2g0091501 RchiOBHm_Chr2g0091591
rosa_laevigata RLG00000016231
rosa_multiflora Rmu_sc0002407.1_g000007
rosa_roxburghii Rroxscaffold_2G00149980
rosa_rugosa Rorug02G0018300
rosa_samantha Rh2AG063500 Rh2BG062900 Rh2CG064600 Rh2DG063000
rosa_wichuraiana Rw2G005690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2832
AatII GACGTC 1 cut(s) 344
Acc36I ACCTGC 4 cut(s) 144, 789, 1065, 2832
AccB1I GGYRCC 1 cut(s) 1518
AccBSI CCGCTC 2 cut(s) 244, 686
AccI GTMKAC 1 cut(s) 198
AccII CGCG 4 cut(s) 105, 2530, 2784, 2786
AccIII TCCGGA 1 cut(s) 699
AclWI GGATC 5 cut(s) 199, 1112, 2086, 2347, 2360
AcoI YGGCCR 1 cut(s) 1039
AcsI RAATTY 6 cut(s) 283, 619, 984, 2084, 2648, 2854
AcuI CTGAAG 5 cut(s) 311, 1764, 1820, 2453, 2945
AcvI CACGTG 1 cut(s) 1328
AcyI GRCGYC 2 cut(s) 341, 380
AfaI GTAC 4 cut(s) 814, 1269, 2155, 2749
AfiI CCNNNNNNNGG 7 cut(s) 92, 151, 262, 451, 480, 1084, 2850
AflIII ACRYGT 2 cut(s) 2528, 2881
AhlI ACTAGT 1 cut(s) 1270
AjnI CCWGG 4 cut(s) 879, 959, 1245, 1945
AloI GAACNNNNNNTCC 2 cut(s) 2261, 2293
Alw21I GWGCWC 5 cut(s) 230, 435, 1578, 1764, 2328
Alw26I GTCTC 5 cut(s) 12, 749, 1246, 1563, 2900
AlwI GGATC 5 cut(s) 199, 1112, 2086, 2347, 2360
AlwNI CAGNNNCTG 2 cut(s) 1384, 2336
Ama87I CYCGRG 1 cut(s) 2477
Aor13HI TCCGGA 1 cut(s) 699
AoxI GGCC 8 cut(s) 3, 160, 232, 717, 1039, 1473, 2007, 2593
ApaI GGGCCC 1 cut(s) 164
ApeKI GCWGC 6 cut(s) 503, 1067, 1552, 1637, 2212, 2912
ApoI RAATTY 6 cut(s) 283, 619, 984, 2084, 2648, 2854
ArsI GACNNNNNNTTYG 6 cut(s) 11, 43, 1046, 1078, 2923, 2955
AseI ATTAAT 1 cut(s) 2202
AspLEI GCGC 4 cut(s) 105, 1407, 2784, 2786
AsuC2I CCSGG 3 cut(s) 93, 125, 418
AsuHPI GGTGA 2 cut(s) 238, 265
AsuII TTCGAA 1 cut(s) 2221
AsuNHI GCTAGC 1 cut(s) 2584
AvaI CYCGRG 1 cut(s) 2477
AvaII GGWCC 5 cut(s) 95, 181, 414, 442, 1499
AxyI CCTNAGG 1 cut(s) 860
BaeGI GKGCMC 1 cut(s) 164
BalI TGGCCA 1 cut(s) 1041
BamHI GGATCC 1 cut(s) 2352
BanI GGYRCC 1 cut(s) 1518
BanII GRGCYC 7 cut(s) 164, 230, 435, 490, 1578, 1764, 2328
BarI GAAGNNNNNNTAC 2 cut(s) 2918, 2950
BauI CACGAG 1 cut(s) 1289
BbrPI CACGTG 1 cut(s) 1328
BbsI GAAGAC 7 cut(s) 138, 285, 962, 1517, 1973, 1979, 2936
Bbv12I GWGCWC 5 cut(s) 230, 435, 1578, 1764, 2328
BbvI GCAGC 6 cut(s) 490, 1079, 1539, 1624, 2224, 2924
BceAI ACGGC 3 cut(s) 393, 649, 2446
BcgI CGANNNNNNTGC 4 cut(s) 752, 786, 2201, 2235
BciT130I CCWGG 4 cut(s) 881, 961, 1247, 1947
BciVI GTATCC 1 cut(s) 663
BcnI CCSGG 3 cut(s) 93, 125, 418
BcoDI GTCTC 5 cut(s) 12, 749, 1246, 1563, 2900
BcuI ACTAGT 1 cut(s) 1270
BfaI CTAG 4 cut(s) 209, 1271, 2064, 2585
BfmI CTRYAG 4 cut(s) 796, 2210, 2910, 2954
BfoI RGCGCY 1 cut(s) 1408
BfuAI ACCTGC 4 cut(s) 144, 789, 1065, 2832
BfuI GTATCC 1 cut(s) 663
BglII AGATCT 1 cut(s) 1657
BisI GCNGC 6 cut(s) 504, 1068, 1553, 1638, 2213, 2913
BlpI GCTNAGC 1 cut(s) 1758
BlsI GCNGC 6 cut(s) 505, 1069, 1554, 1639, 2214, 2914
BmcAI AGTACT 1 cut(s) 1269
Bme1390I CCNGG 7 cut(s) 93, 125, 418, 881, 961, 1247, 1947
Bme18I GGWCC 5 cut(s) 95, 181, 414, 442, 1499
BmeT110I CYCGRG 1 cut(s) 2477
BmiI GGNNCC 9 cut(s) 34, 46, 97, 162, 217, 443, 718, 1520, 2354
BmrFI CCNGG 7 cut(s) 93, 125, 418, 881, 961, 1247, 1947
BmrI ACTGGG 3 cut(s) 283, 844, 1172
BmsI GCATC 7 cut(s) 175, 1090, 1218, 1716, 1951, 2342, 2428
BmtI GCTAGC 1 cut(s) 2588
BmuI ACTGGG 3 cut(s) 283, 844, 1172
BpiI GAAGAC 7 cut(s) 138, 285, 962, 1517, 1973, 1979, 2936
BpmI CTGGAG 3 cut(s) 569, 2610, 2813
Bpu10I CCTNAGC 1 cut(s) 229
Bpu1102I GCTNAGC 1 cut(s) 1758
Bpu14I TTCGAA 1 cut(s) 2221
BpuEI CTTGAG 1 cut(s) 1562
BpuMI CCSGG 3 cut(s) 93, 125, 418
Bsa29I ATCGAT 1 cut(s) 1024
BsaAI YACGTR 3 cut(s) 713, 1328, 2882
BsaHI GRCGYC 2 cut(s) 341, 380
BsaJI CCNNGG 6 cut(s) 720, 732, 960, 970, 1245, 1246
BsaWI WCCGGW 2 cut(s) 279, 699
BsaXI ACNNNNNCTCC 4 cut(s) 1226, 1256, 2283, 2313
Bsc4I CCNNNNNNNGG 7 cut(s) 92, 151, 262, 451, 480, 1084, 2850
Bse21I CCTNAGG 1 cut(s) 860
Bse3DI GCAATG 1 cut(s) 2828
BseAI TCCGGA 1 cut(s) 699
BseBI CCWGG 4 cut(s) 881, 961, 1247, 1947
BseCI ATCGAT 1 cut(s) 1024
BseDI CCNNGG 6 cut(s) 720, 732, 960, 970, 1245, 1246
BseLI CCNNNNNNNGG 7 cut(s) 92, 151, 262, 451, 480, 1084, 2850
BseMI GCAATG 1 cut(s) 2828
BsePI GCGCGC 1 cut(s) 2782
BseRI GAGGAG 4 cut(s) 628, 678, 2146, 2651
BseSI GKGCMC 1 cut(s) 164
BseXI GCAGC 6 cut(s) 490, 1079, 1539, 1624, 2224, 2924
BsgI GTGCAG 1 cut(s) 1685
Bsh1236I CGCG 4 cut(s) 105, 2530, 2784, 2786
Bsh1285I CGRYCG 2 cut(s) 129, 280
BshFI GGCC 8 cut(s) 5, 162, 234, 719, 1041, 1475, 2009, 2595
BshNI GGYRCC 1 cut(s) 1518
BshVI ATCGAT 1 cut(s) 1024
BsiEI CGRYCG 2 cut(s) 129, 280
BsiHKAI GWGCWC 5 cut(s) 230, 435, 1578, 1764, 2328
BsiHKCI CYCGRG 1 cut(s) 2477
BsiSI CCGG 6 cut(s) 93, 125, 223, 280, 417, 700
BslFI GGGAC 4 cut(s) 50, 167, 654, 2803
BslI CCNNNNNNNGG 7 cut(s) 92, 151, 262, 451, 480, 1084, 2850
BsmAI GTCTC 5 cut(s) 12, 749, 1246, 1563, 2900
BsmBI CGTCTC 1 cut(s) 1563
BsmFI GGGAC 4 cut(s) 50, 167, 654, 2803
BsmI GAATGC 3 cut(s) 1159, 1555, 1916
BsnI GGCC 8 cut(s) 5, 162, 234, 719, 1041, 1475, 2009, 2595
BsoBI CYCGRG 1 cut(s) 2477
Bsp119I TTCGAA 1 cut(s) 2221
Bsp120I GGGCCC 1 cut(s) 160
Bsp1286I GDGCHC 7 cut(s) 164, 230, 435, 490, 1578, 1764, 2328
Bsp13I TCCGGA 1 cut(s) 699
Bsp143I GATC 8 cut(s) 204, 270, 1117, 1134, 1352, 1657, 2078, 2352
Bsp1720I GCTNAGC 1 cut(s) 1758
Bsp19I CCATGG 1 cut(s) 732
BspANI GGCC 8 cut(s) 5, 162, 234, 719, 1041, 1475, 2009, 2595
BspDI ATCGAT 1 cut(s) 1024
BspEI TCCGGA 1 cut(s) 699
BspFNI CGCG 4 cut(s) 105, 2530, 2784, 2786
BspHI TCATGA 2 cut(s) 948, 2547
BspLI GGNNCC 9 cut(s) 34, 46, 97, 162, 217, 443, 718, 1520, 2354
BspMAI CTGCAG 3 cut(s) 800, 2214, 2914
BspMI ACCTGC 4 cut(s) 144, 789, 1065, 2832
BspOI GCTAGC 1 cut(s) 2588
BspPI GGATC 5 cut(s) 199, 1112, 2086, 2347, 2360
BspQI GCTCTTC 2 cut(s) 918, 2812
BspT104I TTCGAA 1 cut(s) 2221
BspT107I GGYRCC 1 cut(s) 1518
BsrBI CCGCTC 2 cut(s) 244, 686
BsrDI GCAATG 1 cut(s) 2828
BssECI CCNNGG 6 cut(s) 720, 732, 960, 970, 1245, 1246
BssHII GCGCGC 1 cut(s) 2782
BssMI GATC 8 cut(s) 204, 270, 1117, 1134, 1352, 1657, 2078, 2352
BssNI GRCGYC 2 cut(s) 341, 380
BssSI CACGAG 1 cut(s) 1289
BssT1I CCWWGG 3 cut(s) 720, 732, 970
Bst2BI CACGAG 1 cut(s) 1289
Bst2UI CCWGG 4 cut(s) 881, 961, 1247, 1947
Bst4CI ACNGT 9 cut(s) 196, 414, 817, 1196, 1240, 1621, 2509, 2722, 2747
Bst6I CTCTTC 5 cut(s) 313, 918, 1298, 2240, 2812
BstACI GRCGYC 2 cut(s) 341, 380
BstBAI YACGTR 3 cut(s) 713, 1328, 2882
BstBI TTCGAA 1 cut(s) 2221
BstC8I GCNNGC 7 cut(s) 164, 220, 796, 1764, 2055, 2586, 2784
BstDSI CCRYGG 1 cut(s) 732
BstFNI CGCG 4 cut(s) 105, 2530, 2784, 2786
BstH2I RGCGCY 1 cut(s) 1408
BstHHI GCGC 4 cut(s) 105, 1407, 2784, 2786
BstKTI GATC 8 cut(s) 207, 273, 1120, 1137, 1355, 1660, 2081, 2355
BstMAI GTCTC 5 cut(s) 12, 749, 1246, 1563, 2900
BstMBI GATC 8 cut(s) 204, 270, 1117, 1134, 1352, 1657, 2078, 2352
BstMCI CGRYCG 2 cut(s) 129, 280
BstMWI GCNNNNNNNGC 9 cut(s) 356, 550, 559, 1404, 1754, 1763, 1874, 2601, 2950
BstNI CCWGG 4 cut(s) 881, 961, 1247, 1947
BstNSI RCATGY 1 cut(s) 831
BstSCI CCNGG 7 cut(s) 91, 123, 416, 879, 959, 1245, 1945
BstSFI CTRYAG 4 cut(s) 796, 2210, 2910, 2954
BstSLI GKGCMC 1 cut(s) 164
BstUI CGCG 4 cut(s) 105, 2530, 2784, 2786
BstV1I GCAGC 6 cut(s) 490, 1079, 1539, 1624, 2224, 2924
BstV2I GAAGAC 7 cut(s) 138, 285, 962, 1517, 1973, 1979, 2936
BstX2I RGATCY 4 cut(s) 204, 1117, 1657, 2352
BstYI RGATCY 4 cut(s) 204, 1117, 1657, 2352
Bsu15I ATCGAT 1 cut(s) 1024
Bsu36I CCTNAGG 1 cut(s) 860
BsuI GTATCC 1 cut(s) 663
BsuRI GGCC 8 cut(s) 5, 162, 234, 719, 1041, 1475, 2009, 2595
BsuTUI ATCGAT 1 cut(s) 1024
BtgI CCRYGG 1 cut(s) 732
BtsIMutI CAGTG 6 cut(s) 832, 1209, 1509, 1606, 2612, 2718
BveI ACCTGC 4 cut(s) 144, 789, 1065, 2832
Cac8I GCNNGC 7 cut(s) 164, 220, 796, 1764, 2055, 2586, 2784
CaiI CAGNNNCTG 2 cut(s) 1384, 2336
CciI TCATGA 2 cut(s) 948, 2547
CfoI GCGC 4 cut(s) 105, 1407, 2784, 2786
ClaI ATCGAT 1 cut(s) 1024
CpoI CGGWCCG 1 cut(s) 414
CseI GACGC 3 cut(s) 94, 369, 2519
Csp6I GTAC 4 cut(s) 813, 1268, 2154, 2748
CspI CGGWCCG 1 cut(s) 414
CviQI GTAC 4 cut(s) 813, 1268, 2154, 2748
DpnI GATC 8 cut(s) 206, 272, 1119, 1136, 1354, 1659, 2080, 2354
DpnII GATC 8 cut(s) 204, 270, 1117, 1134, 1352, 1657, 2078, 2352
DraI TTTAAA 1 cut(s) 2647
EaeI YGGCCR 1 cut(s) 1039
Eam1104I CTCTTC 5 cut(s) 313, 918, 1298, 2240, 2812
EarI CTCTTC 5 cut(s) 313, 918, 1298, 2240, 2812
EciI GGCGGA 1 cut(s) 1100
Ecl136II GAGCTC 5 cut(s) 228, 433, 1576, 1762, 2326
Eco130I CCWWGG 3 cut(s) 720, 732, 970
Eco24I GRGCYC 7 cut(s) 164, 230, 435, 490, 1578, 1764, 2328
Eco32I GATATC 1 cut(s) 1282
Eco47I GGWCC 5 cut(s) 95, 181, 414, 442, 1499
Eco53kI GAGCTC 5 cut(s) 228, 433, 1576, 1762, 2326
Eco57I CTGAAG 5 cut(s) 311, 1764, 1820, 2453, 2945
Eco72I CACGTG 1 cut(s) 1328
Eco81I CCTNAGG 1 cut(s) 860
Eco88I CYCGRG 1 cut(s) 2477
EcoICRI GAGCTC 5 cut(s) 228, 433, 1576, 1762, 2326
EcoO109I RGGNCCY 2 cut(s) 717, 2594
EcoRI GAATTC 2 cut(s) 283, 2854
EcoRII CCWGG 4 cut(s) 879, 959, 1245, 1945
EcoRV GATATC 1 cut(s) 1282
EcoT14I CCWWGG 3 cut(s) 720, 732, 970
EcoT22I ATGCAT 1 cut(s) 1944
EcoT38I GRGCYC 7 cut(s) 164, 230, 435, 490, 1578, 1764, 2328
ErhI CCWWGG 3 cut(s) 720, 732, 970
Esp3I CGTCTC 1 cut(s) 1563
FalI AAGNNNNNCTT 2 cut(s) 627, 659
FaqI GGGAC 4 cut(s) 50, 167, 654, 2803
FauI CCCGC 5 cut(s) 171, 264, 432, 1697, 2609
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 6 cut(s) 504, 1068, 1553, 1638, 2213, 2913
FriOI GRGCYC 7 cut(s) 164, 230, 435, 490, 1578, 1764, 2328
Fsp4HI GCNGC 6 cut(s) 504, 1068, 1553, 1638, 2213, 2913
FspBI CTAG 4 cut(s) 209, 1271, 2064, 2585
GlaI GCGC 4 cut(s) 104, 1406, 2783, 2785
GluI GCNGC 6 cut(s) 504, 1068, 1553, 1638, 2213, 2913
GsuI CTGGAG 3 cut(s) 569, 2610, 2813
HaeII RGCGCY 1 cut(s) 1408
HaeIII GGCC 8 cut(s) 5, 162, 234, 719, 1041, 1475, 2009, 2595
HapII CCGG 6 cut(s) 93, 125, 223, 280, 417, 700
HgaI GACGC 3 cut(s) 94, 369, 2519
HhaI GCGC 4 cut(s) 105, 1407, 2784, 2786
Hin1I GRCGYC 2 cut(s) 341, 380
Hin6I GCGC 4 cut(s) 103, 1405, 2782, 2784
HinP1I GCGC 4 cut(s) 103, 1405, 2782, 2784
HincII GTYRAC 5 cut(s) 1339, 1608, 1813, 2505, 2715
HindII GTYRAC 5 cut(s) 1339, 1608, 1813, 2505, 2715
HindIII AAGCTT 2 cut(s) 603, 2553
HpaII CCGG 6 cut(s) 93, 125, 223, 280, 417, 700
HphI GGTGA 2 cut(s) 238, 265
Hpy166II GTNNAC 9 cut(s) 121, 199, 410, 1339, 1608, 1813, 2505, 2512, 2715
Hpy8I GTNNAC 9 cut(s) 121, 199, 410, 1339, 1608, 1813, 2505, 2512, 2715
Hpy99I CGWCG 2 cut(s) 110, 385
HpyCH4III ACNGT 9 cut(s) 196, 414, 817, 1196, 1240, 1621, 2509, 2722, 2747
HpyCH4IV ACGT 4 cut(s) 341, 712, 1327, 2881
HpyF10VI GCNNNNNNNGC 9 cut(s) 356, 550, 559, 1404, 1754, 1763, 1874, 2601, 2950
HpySE526I ACGT 4 cut(s) 341, 712, 1327, 2881
Hsp92I GRCGYC 2 cut(s) 341, 380
HspAI GCGC 4 cut(s) 103, 1405, 2782, 2784
Kpn2I TCCGGA 1 cut(s) 699
Kzo9I GATC 8 cut(s) 204, 270, 1117, 1134, 1352, 1657, 2078, 2352
LguI GCTCTTC 2 cut(s) 918, 2812
Lsp1109I GCAGC 6 cut(s) 490, 1079, 1539, 1624, 2224, 2924
LweI GCATC 7 cut(s) 175, 1090, 1218, 1716, 1951, 2342, 2428
MaeI CTAG 4 cut(s) 209, 1271, 2064, 2585
MaeII ACGT 4 cut(s) 341, 712, 1327, 2881
MaeIII GTNAC 6 cut(s) 321, 555, 786, 1030, 1328, 2115
MalI GATC 8 cut(s) 206, 272, 1119, 1136, 1354, 1659, 2080, 2354
MbiI CCGCTC 2 cut(s) 244, 686
MboI GATC 8 cut(s) 204, 270, 1117, 1134, 1352, 1657, 2078, 2352
MflI RGATCY 4 cut(s) 204, 1117, 1657, 2352
MhlI GDGCHC 7 cut(s) 164, 230, 435, 490, 1578, 1764, 2328
MlsI TGGCCA 1 cut(s) 1041
MluI ACGCGT 1 cut(s) 2528
MluNI TGGCCA 1 cut(s) 1041
MlyI GAGTC 5 cut(s) 72, 170, 1232, 1603, 2782
MmeI TCCRAC 8 cut(s) 193, 198, 243, 846, 1148, 2128, 2380, 2861
Mox20I TGGCCA 1 cut(s) 1041
Mph1103I ATGCAT 1 cut(s) 1944
MroI TCCGGA 1 cut(s) 699
MscI TGGCCA 1 cut(s) 1041
MseI TTAA 4 cut(s) 2202, 2646, 2699, 2724
MslI CAYNNNNRTG 2 cut(s) 1026, 2898
Msp20I TGGCCA 1 cut(s) 1041
MspA1I CMGCKG 1 cut(s) 2693
MspI CCGG 6 cut(s) 93, 125, 223, 280, 417, 700
MspR9I CCNGG 7 cut(s) 93, 125, 418, 881, 961, 1247, 1947
Mva1269I GAATGC 3 cut(s) 1159, 1555, 1916
MvaI CCWGG 4 cut(s) 881, 961, 1247, 1947
MvnI CGCG 4 cut(s) 105, 2530, 2784, 2786
MwoI GCNNNNNNNGC 9 cut(s) 356, 550, 559, 1404, 1754, 1763, 1874, 2601, 2950
NciI CCSGG 3 cut(s) 93, 125, 418
NcoI CCATGG 1 cut(s) 732
NdeII GATC 8 cut(s) 204, 270, 1117, 1134, 1352, 1657, 2078, 2352
NheI GCTAGC 1 cut(s) 2584
NlaIV GGNNCC 9 cut(s) 34, 46, 97, 162, 217, 443, 718, 1520, 2354
NmuCI GTSAC 4 cut(s) 321, 786, 1328, 2115
NsiI ATGCAT 1 cut(s) 1944
NspI RCATGY 1 cut(s) 831
NspV TTCGAA 1 cut(s) 2221
PaeR7I CTCGAG 1 cut(s) 2477
PagI TCATGA 2 cut(s) 948, 2547
PaqCI CACCTGC 1 cut(s) 2832
PasI CCCWGGG 1 cut(s) 1246
PauI GCGCGC 1 cut(s) 2782
PciSI GCTCTTC 2 cut(s) 918, 2812
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 347
PctI GAATGC 3 cut(s) 1159, 1555, 1916
PfoI TCCNGGA 1 cut(s) 879
PkrI GCNGC 6 cut(s) 505, 1069, 1554, 1639, 2214, 2914
PleI GAGTC 5 cut(s) 71, 170, 1231, 1603, 2782
PmaCI CACGTG 1 cut(s) 1328
PmlI CACGTG 1 cut(s) 1328
PpsI GAGTC 5 cut(s) 71, 170, 1231, 1603, 2782
Ppu21I YACGTR 3 cut(s) 713, 1328, 2882
PshBI ATTAAT 1 cut(s) 2202
Psp124BI GAGCTC 5 cut(s) 230, 435, 1578, 1764, 2328
Psp6I CCWGG 4 cut(s) 879, 959, 1245, 1945
PspCI CACGTG 1 cut(s) 1328
PspGI CCWGG 4 cut(s) 879, 959, 1245, 1945
PspN4I GGNNCC 9 cut(s) 34, 46, 97, 162, 217, 443, 718, 1520, 2354
PspOMI GGGCCC 1 cut(s) 160
PspXI VCTCGAGB 1 cut(s) 2477
PsrI GAACNNNNNNTAC 2 cut(s) 2017, 2049
PstI CTGCAG 3 cut(s) 800, 2214, 2914
PstNI CAGNNNCTG 2 cut(s) 1384, 2336
PsuI RGATCY 4 cut(s) 204, 1117, 1657, 2352
PteI GCGCGC 1 cut(s) 2782
PvuII CAGCTG 1 cut(s) 2693
RsaI GTAC 4 cut(s) 814, 1269, 2155, 2749
RsaNI GTAC 4 cut(s) 813, 1268, 2154, 2748
RseI CAYNNNNRTG 2 cut(s) 1026, 2898
Rsr2I CGGWCCG 1 cut(s) 414
RsrII CGGWCCG 1 cut(s) 414
SacI GAGCTC 5 cut(s) 230, 435, 1578, 1764, 2328
SapI GCTCTTC 2 cut(s) 918, 2812
SaqAI TTAA 4 cut(s) 2202, 2646, 2699, 2724
SatI GCNGC 6 cut(s) 504, 1068, 1553, 1638, 2213, 2913
Sau3AI GATC 8 cut(s) 204, 270, 1117, 1134, 1352, 1657, 2078, 2352
SbfI CCTGCAGG 1 cut(s) 800
ScaI AGTACT 1 cut(s) 1269
SchI GAGTC 5 cut(s) 72, 170, 1232, 1603, 2782
ScrFI CCNGG 7 cut(s) 93, 125, 418, 881, 961, 1247, 1947
SdaI CCTGCAGG 1 cut(s) 800
SduI GDGCHC 7 cut(s) 164, 230, 435, 490, 1578, 1764, 2328
SfaNI GCATC 7 cut(s) 175, 1090, 1218, 1716, 1951, 2342, 2428
SfcI CTRYAG 4 cut(s) 796, 2210, 2910, 2954
Sfr274I CTCGAG 1 cut(s) 2477
SfuI TTCGAA 1 cut(s) 2221
SinI GGWCC 5 cut(s) 95, 181, 414, 442, 1499
SlaI CTCGAG 1 cut(s) 2477
SmiMI CAYNNNNRTG 2 cut(s) 1026, 2898
SmlI CTYRAG 2 cut(s) 1577, 2477
SmoI CTYRAG 2 cut(s) 1577, 2477
SpeI ACTAGT 1 cut(s) 1270
Sse8387I CCTGCAGG 1 cut(s) 800
SspI AATATT 1 cut(s) 1219
SspMI CTAG 4 cut(s) 209, 1271, 2064, 2585
SstI GAGCTC 5 cut(s) 230, 435, 1578, 1764, 2328
StyD4I CCNGG 7 cut(s) 91, 123, 416, 879, 959, 1245, 1945
StyI CCWWGG 3 cut(s) 720, 732, 970
TaaI ACNGT 9 cut(s) 196, 414, 817, 1196, 1240, 1621, 2509, 2722, 2747
TaiI ACGT 4 cut(s) 344, 715, 1330, 2884
TaqI TCGA 8 cut(s) 129, 1024, 1226, 1677, 2003, 2029, 2221, 2478
TaqII GACCGA 1 cut(s) 482
TatI WGTACW 2 cut(s) 1267, 2153
Tru1I TTAA 4 cut(s) 2202, 2646, 2699, 2724
Tru9I TTAA 4 cut(s) 2202, 2646, 2699, 2724
TscAI CASTG 6 cut(s) 839, 1216, 1509, 1606, 2619, 2725
TseFI GTSAC 4 cut(s) 321, 786, 1328, 2115
TseI GCWGC 6 cut(s) 503, 1067, 1552, 1637, 2212, 2912
Tsp45I GTSAC 4 cut(s) 321, 786, 1328, 2115
TspDTI ATGAA 9 cut(s) 615, 918, 965, 1010, 1917, 2029, 2078, 2412, 2564
TspGWI ACGGA 3 cut(s) 840, 1435, 2555
TspRI CASTG 6 cut(s) 839, 1216, 1509, 1606, 2619, 2725
VpaK11BI GGWCC 5 cut(s) 95, 181, 414, 442, 1499
VspI ATTAAT 1 cut(s) 2202
XapI RAATTY 6 cut(s) 283, 619, 984, 2084, 2648, 2854
XceI RCATGY 1 cut(s) 831
XcmI CCANNNNNNNNNTGG 2 cut(s) 839, 1078
XhoI CTCGAG 1 cut(s) 2477
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 4 cut(s) 209, 1271, 2064, 2585
ZraI GACGTC 1 cut(s) 342
ZrmI AGTACT 1 cut(s) 1269
Zsp2I ATGCAT 1 cut(s) 1944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.