Rh2DG063000

Plant family of unknown function (DUF810)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011157
Physical Location & Seq
Forward (+)
4644463 .. 4648210
3748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG063000.1

Sequence Viewer

Length: 2973 bp
ATGGCCCAGATTATCAGAGACAGAGTTTTCGGCTCCTTTGGTGGTTCCAGACGACACAACAGAGTCCCTATTCCAATTCCAATTCCAGAACCCGAACCCGAACCCGAACCCGAACGAGTCGTCATGCCCGTTTACCCGGTCGAAGACCTGCCCAACCCGTTCGGGGACTTGGGCCCGCATCTCTCGGACTCGGACCTCCGAGAAACCGTCTACGAGATCCTAGTCGGAGCCTGCCGGAGCTCCGGCCCGAAACCGCTCACCTACACCCCGCAGTCGGAGAAGATCACCGACCGGAATTCCCAGTCTTCTTCTTCTTCTTCTTCTTCCTCGCTGTCGTCACTTCAGAGGTCGCTGACGTCATCGGCGAAGAAGGCTCTGGGTATGCAGCAAACGGCGTCGTCTAAGAGGCTCGGTGGGAGTAGTAAACGGTCCGGGTCGGTTTTTGAGCTCATGCGGGTCCAGATGAGGGTTTCGGAGCAGACCGATACCCGAATCAGGAGAGCCCTTCTCAGAGTTGCTGCTGGTCAGCTCGGACGAAAGATAGAGAGCATGGTTTTGCCTCTGGAGCTGTTACAGCATTTCAGGTCGTTAGATTTTGCGAATCAGCAGGAGTATGAAGCTTGGCAGAGGAGGAATTTGAAGGTTCTTGAAGTGGGACTCCTGGTGTATCCTCATATGCCGTTGGATAGAAAAGACACCGCTCCTCAACAGCTCCGGAAGATTATTCGTGGGGCCTTGGAGAAACCCATGGAAACTGGAAAGAACACCGAGACAATGCAAGTTCTCCGCAGTCTTGTTATGTCGCTTGCCTGCAGATCGTTTGATGGTACTGTTTCCGACACATGCCACTGGGCAGATGGGTTTCCGTTGAATCTCAGGCTCTACCAAAAGCTCCTGGAATCGTGTTTTGACCTGAATGAAGAAACTTCTATTATTGAAGAGCTTGATGAGGTTTTAGAAGTCATGAAGAAGACCTGGGTAGTCCTTGGAATAAACCAAATTCTGCATAATCTGTGTTTCGCATGGGTTTTATTTCATCGATATGTTACAACTGGCCAAGTGGATAATGACCTGCTGTTTGCAGCCAATAACCTCTTGGCGGAAGTTCAGCAAGATGCCAATGCCACAAAAGATCCATCTTATTTGAAGATCGTAAGTTCAACATTGAATGCAATACTGGGTTGGACTGAGAGAAGGCTTCTTGCCTACCGTAATGTTTTCAACACTGGCAATATTGAGTCGATGCAAAACATTGTCTCCCTGGGGGTATTATCAGCAAAAGTACTAGTAGAGGATATCTCTCACGAATATCGTAGGAAGAGAAAAGAAGTTGATGTGGCACATGACAGGGTTGACACCTACATAAGATCATCAATACGCACCGCTTTTGCTCAGAAACTGGAAAAAGTGGGCTCCAACAAGCGCCTATCAAAGAGCCAAAAGAATCGTATTCCTACCTTGTCCGTCCTTGCAGCAGAAGTTAGCGAACTGGCCTTTAGTGAGAAAGATATATTTGGTCCAGTGTTGAAGAGATGGCACCCTTTTGCAACGGGTGTTGCTATGGCTACGCTGCATTCTTGCTATGGAGACGAGCTCAAGCAATTTGTGGATGGTATCAGTGAGTTGACTCCTGATACTGTACAAGTACTAAAAGCTGCTGACAAATTGGAGAAAGATCTTGTGCAGATTGCTGTCGAAGATTCAGTAGATAGTGAGGATGGCGGGAAATCCATCATACAGGAGATGCCTCCTTACGAGGCTGAAGCTGTAATGGCTGAGCTTGCGAAGGCTTGGATAAGGACAAGATTGGACAGGCTGAAGGAATGGGTTGACAGAAATCTGCAACAAGAGGTCTGGAATCCACAAGCAAACAAAGAATGCTTTGCTCCTTCTGCCATTGAAGTTTTACGCATTGTAGATGAAACATTGGAAGCATTTTTTATGTTGCCTATACCAATGCATCCTGGCTTGATTCCAGAATTGAAGACTGGTCTTGATAGATGTCTTCAATACTATGTATCCAAGGCCAAGACTGGCTGTGGAACCCGAATTACCTTCATTCCCACTATGCCTGCTTTGACTAGATGTTCAGCGGGATCAAAATTTCATGGTGTATTCAAGAAGAAGGAAAGGTCACATATAAACCAGAGGAGGAAATCTCAGGTTGGAAGTACAAATGGGGATGGTTCGTTTGGGATTCCGCAACTTTGTGTTCGCATTAATACTCTGCAGCATATTCGAATGGAGTTGGGAGTTTTTGAGAAGAGGATTGTTGCTCATCTTGGGAACTTGGAATCCACTCAAATGGGAGATTTTGCGAATGGGATGGGCAAAATGTTTGAGCTCTCAGCACCTGCTTGTTTAGAAGGGATCCAACAACTTTGTGAGGCAACAGCTTACAAGGTTGTTTTCCATGAACTAAGTCATGTTCTTTGGGATGGCCTGTATAATGTGGATCCCTCATGTAGAATTGAGCCTTTTCTTCAGGAACTTGAGCAATTTTTGGAGATTATCTCGTCAACAGTTCACAACAGAGTTAGAACGCGTCTTATTACGGATGTCATGAAAGCTTCTTTTGACGGGTTCTTGTTGGTTTTGCTAGCTGGAGGCCCTTCCCGCAGTTTCACAGTGAGAGATTCTAACATAATAGAGGAGGATTTTAAATTTCTGACTGATTTGTTCTGGTCAAACGGGGATGGATTACCAGCTGATTTAATAGCTAAGTTGTCAACCACTGTTAAGGACATCCTTCCTCTCTACCGTACTGAGACTGATAGCCTTATTGAAAAGTTCAAGCGCACGACTCTGGAGAACTATGGCTCTTCTGCGAAGTCCCACCTGCCATTGCCTCCAACTTCCGATGAGTGGAATTCCAATGACCCAAATACACTTCTACGTGTCTTATGTCATAGGAATGATGAGACTGCAGCCAAGTTTCTGAAGAAGACTTACAATTTGCCTAAAAAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF810

Protein Analysis

990

Amino Acids

111.48

Weight (kDa)

7.29

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PATROL1 PF25761 592 - 989 1.9e-165 PATROL1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20010 AT2G20010
fragaria_vesca FvH4_1g05840
malus_domestica MD02G1060400.v1.1 MD15G1193900.v1.1
prunus_persica Prupe.7G222400_v2.0.a1 Prupe.7G222400_v2.0.a1
pyrus_communis pycom02g04960 pycom15g17370
rosa_chinensis RchiOBHm_Chr2g0091501 RchiOBHm_Chr2g0091591
rosa_laevigata RLG00000016231
rosa_multiflora Rmu_sc0002407.1_g000007
rosa_roxburghii Rroxscaffold_2G00149980
rosa_rugosa Rorug02G0018300
rosa_samantha Rh2AG063500 Rh2BG062900 Rh2CG064600 Rh2DG063000
rosa_wichuraiana Rw2G005690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 2362, 2847
AatII GACGTC 1 cut(s) 359
Acc36I ACCTGC 4 cut(s) 156, 1080, 2362, 2847
AccB1I GGYRCC 1 cut(s) 1536
AccBSI CCGCTC 2 cut(s) 256, 701
AccI GTMKAC 1 cut(s) 210
AccII CGCG 1 cut(s) 2545
AccIII TCCGGA 1 cut(s) 714
AclWI GGATC 7 cut(s) 211, 1127, 2104, 2365, 2378, 2450, 2463
AcoI YGGCCR 1 cut(s) 1054
AcsI RAATTY 6 cut(s) 295, 634, 999, 2102, 2663, 2869
AcuI CTGAAG 5 cut(s) 326, 1782, 1838, 2468, 2960
AcyI GRCGYC 2 cut(s) 356, 395
AfaI GTAC 6 cut(s) 829, 1284, 1641, 1647, 2173, 2764
AfiI CCNNNNNNNGG 6 cut(s) 163, 274, 466, 495, 1099, 2865
AflIII ACRYGT 2 cut(s) 2543, 2896
AhlI ACTAGT 1 cut(s) 1285
AjnI CCWGG 5 cut(s) 660, 894, 974, 1260, 1963
AloI GAACNNNNNNTCC 2 cut(s) 2279, 2311
Alw21I GWGCWC 4 cut(s) 242, 450, 1596, 2346
Alw26I GTCTC 6 cut(s) 12, 764, 1261, 1581, 2762, 2915
AlwI GGATC 7 cut(s) 211, 1127, 2104, 2365, 2378, 2450, 2463
AlwNI CAGNNNCTG 2 cut(s) 1399, 2354
Aor13HI TCCGGA 1 cut(s) 714
AoxI GGCC 9 cut(s) 3, 172, 244, 732, 1054, 1491, 2025, 2440, 2608
ApaI GGGCCC 1 cut(s) 176
ApeKI GCWGC 8 cut(s) 385, 518, 1082, 1472, 1570, 1655, 2230, 2927
ApoI RAATTY 6 cut(s) 295, 634, 999, 2102, 2663, 2869
ArsI GACNNNNNNTTYG 8 cut(s) 11, 43, 105, 137, 1061, 1093, 2938, 2970
AseI ATTAAT 1 cut(s) 2220
AspLEI GCGC 2 cut(s) 1425, 2799
AsuC2I CCSGG 2 cut(s) 137, 433
AsuHPI GGTGA 2 cut(s) 250, 277
AsuII TTCGAA 1 cut(s) 2239
AsuNHI GCTAGC 1 cut(s) 2599
AvaII GGWCC 4 cut(s) 193, 429, 457, 1517
BaeGI GKGCMC 1 cut(s) 176
BalI TGGCCA 1 cut(s) 1056
BamHI GGATCC 2 cut(s) 2370, 2455
BanI GGYRCC 1 cut(s) 1536
BanII GRGCYC 7 cut(s) 176, 242, 450, 505, 1415, 1596, 2346
BarI GAAGNNNNNNTAC 2 cut(s) 2933, 2965
BbsI GAAGAC 6 cut(s) 150, 297, 977, 1991, 1997, 2951
Bbv12I GWGCWC 4 cut(s) 242, 450, 1596, 2346
BbvI GCAGC 8 cut(s) 397, 505, 1094, 1484, 1557, 1642, 2242, 2939
BceAI ACGGC 2 cut(s) 408, 664
BcgI CGANNNNNNTGC 2 cut(s) 2219, 2253
BciT130I CCWGG 5 cut(s) 662, 896, 976, 1262, 1965
BciVI GTATCC 2 cut(s) 678, 2029
BcnI CCSGG 2 cut(s) 137, 433
BcoDI GTCTC 6 cut(s) 12, 764, 1261, 1581, 2762, 2915
BcuI ACTAGT 1 cut(s) 1285
BfaI CTAG 4 cut(s) 221, 1286, 2082, 2600
BfmI CTRYAG 3 cut(s) 811, 2228, 2925
BfoI RGCGCY 1 cut(s) 1426
BfuAI ACCTGC 4 cut(s) 156, 1080, 2362, 2847
BfuI GTATCC 2 cut(s) 678, 2029
BglII AGATCT 1 cut(s) 1675
BisI GCNGC 8 cut(s) 386, 519, 1083, 1473, 1571, 1656, 2231, 2928
BlpI GCTNAGC 1 cut(s) 1776
BlsI GCNGC 8 cut(s) 387, 520, 1084, 1474, 1572, 1657, 2232, 2929
BmcAI AGTACT 2 cut(s) 1284, 1647
Bme1390I CCNGG 7 cut(s) 137, 433, 662, 896, 976, 1262, 1965
Bme18I GGWCC 4 cut(s) 193, 429, 457, 1517
BmrFI CCNGG 7 cut(s) 137, 433, 662, 896, 976, 1262, 1965
BmrI ACTGGG 3 cut(s) 295, 859, 1187
BmsI GCATC 5 cut(s) 187, 1105, 1233, 1734, 1969
BmtI GCTAGC 1 cut(s) 2603
BmuI ACTGGG 3 cut(s) 295, 859, 1187
BpiI GAAGAC 6 cut(s) 150, 297, 977, 1991, 1997, 2951
BpmI CTGGAG 3 cut(s) 584, 2625, 2828
Bpu1102I GCTNAGC 1 cut(s) 1776
Bpu14I TTCGAA 1 cut(s) 2239
BpuEI CTTGAG 2 cut(s) 1580, 2513
BpuMI CCSGG 2 cut(s) 137, 433
Bsa29I ATCGAT 1 cut(s) 1039
BsaAI YACGTR 1 cut(s) 2897
BsaHI GRCGYC 2 cut(s) 356, 395
BsaJI CCNNGG 7 cut(s) 735, 747, 975, 985, 1260, 1261, 2022
BsaWI WCCGGW 2 cut(s) 291, 714
BsaXI ACNNNNNCTCC 2 cut(s) 1241, 1271
Bsc4I CCNNNNNNNGG 6 cut(s) 163, 274, 466, 495, 1099, 2865
Bse3DI GCAATG 1 cut(s) 2843
BseAI TCCGGA 1 cut(s) 714
BseBI CCWGG 5 cut(s) 662, 896, 976, 1262, 1965
BseCI ATCGAT 1 cut(s) 1039
BseDI CCNNGG 7 cut(s) 735, 747, 975, 985, 1260, 1261, 2022
BseGI GGATG 9 cut(s) 1615, 1723, 1960, 2188, 2331, 2443, 2563, 2701, 2745
BseLI CCNNNNNNNGG 6 cut(s) 163, 274, 466, 495, 1099, 2865
BseMI GCAATG 1 cut(s) 2843
BseMII CTCAG 8 cut(s) 523, 889, 1179, 1406, 1767, 2174, 2361, 2757
BseRI GAGGAG 4 cut(s) 643, 693, 2164, 2666
BseSI GKGCMC 1 cut(s) 176
BseXI GCAGC 8 cut(s) 397, 505, 1094, 1484, 1557, 1642, 2242, 2939
BsgI GTGCAG 1 cut(s) 1703
Bsh1236I CGCG 1 cut(s) 2545
Bsh1285I CGRYCG 2 cut(s) 141, 292
BshFI GGCC 9 cut(s) 5, 174, 246, 734, 1056, 1493, 2027, 2442, 2610
BshNI GGYRCC 1 cut(s) 1536
BshVI ATCGAT 1 cut(s) 1039
BsiEI CGRYCG 2 cut(s) 141, 292
BsiHKAI GWGCWC 4 cut(s) 242, 450, 1596, 2346
BsiSI CCGG 6 cut(s) 137, 235, 243, 292, 432, 715
BslFI GGGAC 4 cut(s) 50, 179, 669, 2818
BslI CCNNNNNNNGG 6 cut(s) 163, 274, 466, 495, 1099, 2865
BsmAI GTCTC 6 cut(s) 12, 764, 1261, 1581, 2762, 2915
BsmBI CGTCTC 1 cut(s) 1581
BsmFI GGGAC 4 cut(s) 50, 179, 669, 2818
BsmI GAATGC 3 cut(s) 1174, 1573, 1883
BsnI GGCC 9 cut(s) 5, 174, 246, 734, 1056, 1493, 2027, 2442, 2610
Bsp119I TTCGAA 1 cut(s) 2239
Bsp120I GGGCCC 1 cut(s) 172
Bsp1286I GDGCHC 7 cut(s) 176, 242, 450, 505, 1415, 1596, 2346
Bsp13I TCCGGA 1 cut(s) 714
Bsp1407I TGTACA 1 cut(s) 1639
Bsp1720I GCTNAGC 1 cut(s) 1776
Bsp19I CCATGG 1 cut(s) 747
BspANI GGCC 9 cut(s) 5, 174, 246, 734, 1056, 1493, 2027, 2442, 2610
BspCNI CTCAG 8 cut(s) 522, 888, 1180, 1405, 1768, 2173, 2360, 2758
BspDI ATCGAT 1 cut(s) 1039
BspEI TCCGGA 1 cut(s) 714
BspFNI CGCG 1 cut(s) 2545
BspHI TCATGA 2 cut(s) 963, 2562
BspMAI CTGCAG 3 cut(s) 815, 2232, 2929
BspMI ACCTGC 4 cut(s) 156, 1080, 2362, 2847
BspOI GCTAGC 1 cut(s) 2603
BspPI GGATC 7 cut(s) 211, 1127, 2104, 2365, 2378, 2450, 2463
BspQI GCTCTTC 2 cut(s) 933, 2827
BspT104I TTCGAA 1 cut(s) 2239
BspT107I GGYRCC 1 cut(s) 1536
BsrBI CCGCTC 2 cut(s) 256, 701
BsrDI GCAATG 1 cut(s) 2843
BsrGI TGTACA 1 cut(s) 1639
BssECI CCNNGG 7 cut(s) 735, 747, 975, 985, 1260, 1261, 2022
BssNI GRCGYC 2 cut(s) 356, 395
BssT1I CCWWGG 4 cut(s) 735, 747, 985, 2022
Bst2UI CCWGG 5 cut(s) 662, 896, 976, 1262, 1965
Bst4CI ACNGT 9 cut(s) 208, 429, 832, 1211, 1639, 2524, 2629, 2737, 2762
Bst6I CTCTTC 5 cut(s) 933, 1313, 1523, 2258, 2827
BstACI GRCGYC 2 cut(s) 356, 395
BstAUI TGTACA 1 cut(s) 1639
BstBAI YACGTR 1 cut(s) 2897
BstBI TTCGAA 1 cut(s) 2239
BstC8I GCNNGC 7 cut(s) 176, 232, 807, 811, 1782, 2073, 2601
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 9 cut(s) 1615, 1723, 1960, 2188, 2331, 2443, 2563, 2701, 2745
BstFNI CGCG 1 cut(s) 2545
BstH2I RGCGCY 1 cut(s) 1426
BstHHI GCGC 2 cut(s) 1425, 2799
BstMAI GTCTC 6 cut(s) 12, 764, 1261, 1581, 2762, 2915
BstMCI CGRYCG 2 cut(s) 141, 292
BstMWI GCNNNNNNNGC 8 cut(s) 371, 565, 574, 1772, 1781, 1892, 2616, 2965
BstNI CCWGG 5 cut(s) 662, 896, 976, 1262, 1965
BstNSI RCATGY 1 cut(s) 846
BstSCI CCNGG 7 cut(s) 135, 431, 660, 894, 974, 1260, 1963
BstSFI CTRYAG 3 cut(s) 811, 2228, 2925
BstSLI GKGCMC 1 cut(s) 176
BstUI CGCG 1 cut(s) 2545
BstV1I GCAGC 8 cut(s) 397, 505, 1094, 1484, 1557, 1642, 2242, 2939
BstV2I GAAGAC 6 cut(s) 150, 297, 977, 1991, 1997, 2951
BstX2I RGATCY 5 cut(s) 216, 1132, 1675, 2370, 2455
BstXI CCANNNNNNTGG 1 cut(s) 2305
BstYI RGATCY 5 cut(s) 216, 1132, 1675, 2370, 2455
Bsu15I ATCGAT 1 cut(s) 1039
BsuI GTATCC 2 cut(s) 678, 2029
BsuRI GGCC 9 cut(s) 5, 174, 246, 734, 1056, 1493, 2027, 2442, 2610
BsuTUI ATCGAT 1 cut(s) 1039
BtgI CCRYGG 1 cut(s) 747
BtsCI GGATG 9 cut(s) 1615, 1723, 1960, 2188, 2331, 2443, 2563, 2701, 2745
BtsIMutI CAGTG 6 cut(s) 847, 1224, 1527, 1624, 2634, 2733
BveI ACCTGC 4 cut(s) 156, 1080, 2362, 2847
Cac8I GCNNGC 7 cut(s) 176, 232, 807, 811, 1782, 2073, 2601
CaiI CAGNNNCTG 2 cut(s) 1399, 2354
CciI TCATGA 2 cut(s) 963, 2562
CfoI GCGC 2 cut(s) 1425, 2799
ClaI ATCGAT 1 cut(s) 1039
CpoI CGGWCCG 1 cut(s) 429
CseI GACGC 2 cut(s) 384, 2534
Csp6I GTAC 6 cut(s) 828, 1283, 1640, 1646, 2172, 2763
CspI CGGWCCG 1 cut(s) 429
CviQI GTAC 6 cut(s) 828, 1283, 1640, 1646, 2172, 2763
DraI TTTAAA 1 cut(s) 2662
EaeI YGGCCR 1 cut(s) 1054
Eam1104I CTCTTC 5 cut(s) 933, 1313, 1523, 2258, 2827
EarI CTCTTC 5 cut(s) 933, 1313, 1523, 2258, 2827
EciI GGCGGA 1 cut(s) 1115
Ecl136II GAGCTC 4 cut(s) 240, 448, 1594, 2344
Eco130I CCWWGG 4 cut(s) 735, 747, 985, 2022
Eco24I GRGCYC 7 cut(s) 176, 242, 450, 505, 1415, 1596, 2346
Eco32I GATATC 1 cut(s) 1297
Eco47I GGWCC 4 cut(s) 193, 429, 457, 1517
Eco53kI GAGCTC 4 cut(s) 240, 448, 1594, 2344
Eco57I CTGAAG 5 cut(s) 326, 1782, 1838, 2468, 2960
EcoICRI GAGCTC 4 cut(s) 240, 448, 1594, 2344
EcoO109I RGGNCCY 2 cut(s) 732, 2609
EcoRI GAATTC 2 cut(s) 295, 2869
EcoRII CCWGG 5 cut(s) 660, 894, 974, 1260, 1963
EcoRV GATATC 1 cut(s) 1297
EcoT14I CCWWGG 4 cut(s) 735, 747, 985, 2022
EcoT22I ATGCAT 1 cut(s) 1962
EcoT38I GRGCYC 7 cut(s) 176, 242, 450, 505, 1415, 1596, 2346
ErhI CCWWGG 4 cut(s) 735, 747, 985, 2022
Esp3I CGTCTC 1 cut(s) 1581
FaqI GGGAC 4 cut(s) 50, 179, 669, 2818
FauI CCCGC 6 cut(s) 183, 276, 447, 1715, 2086, 2624
FauNDI CATATG 1 cut(s) 675
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 8 cut(s) 386, 519, 1083, 1473, 1571, 1656, 2231, 2928
FokI GGATG 9 cut(s) 1622, 1730, 1947, 2195, 2338, 2450, 2570, 2708, 2732
FriOI GRGCYC 7 cut(s) 176, 242, 450, 505, 1415, 1596, 2346
Fsp4HI GCNGC 8 cut(s) 386, 519, 1083, 1473, 1571, 1656, 2231, 2928
FspBI CTAG 4 cut(s) 221, 1286, 2082, 2600
GlaI GCGC 2 cut(s) 1424, 2798
GluI GCNGC 8 cut(s) 386, 519, 1083, 1473, 1571, 1656, 2231, 2928
GsuI CTGGAG 3 cut(s) 584, 2625, 2828
HaeII RGCGCY 1 cut(s) 1426
HaeIII GGCC 9 cut(s) 5, 174, 246, 734, 1056, 1493, 2027, 2442, 2610
HapII CCGG 6 cut(s) 137, 235, 243, 292, 432, 715
HgaI GACGC 2 cut(s) 384, 2534
HhaI GCGC 2 cut(s) 1425, 2799
Hin1I GRCGYC 2 cut(s) 356, 395
Hin6I GCGC 2 cut(s) 1423, 2797
HinP1I GCGC 2 cut(s) 1423, 2797
HincII GTYRAC 5 cut(s) 1354, 1626, 1831, 2520, 2730
HindII GTYRAC 5 cut(s) 1354, 1626, 1831, 2520, 2730
HindIII AAGCTT 2 cut(s) 618, 2568
HpaII CCGG 6 cut(s) 137, 235, 243, 292, 432, 715
HphI GGTGA 2 cut(s) 250, 277
Hpy166II GTNNAC 9 cut(s) 133, 211, 425, 1354, 1626, 1831, 2520, 2527, 2730
Hpy8I GTNNAC 9 cut(s) 133, 211, 425, 1354, 1626, 1831, 2520, 2527, 2730
Hpy99I CGWCG 1 cut(s) 400
HpyCH4III ACNGT 9 cut(s) 208, 429, 832, 1211, 1639, 2524, 2629, 2737, 2762
HpyCH4IV ACGT 2 cut(s) 356, 2896
HpyF10VI GCNNNNNNNGC 8 cut(s) 371, 565, 574, 1772, 1781, 1892, 2616, 2965
HpySE526I ACGT 2 cut(s) 356, 2896
Hsp92I GRCGYC 2 cut(s) 356, 395
HspAI GCGC 2 cut(s) 1423, 2797
Kpn2I TCCGGA 1 cut(s) 714
LguI GCTCTTC 2 cut(s) 933, 2827
Lsp1109I GCAGC 8 cut(s) 397, 505, 1094, 1484, 1557, 1642, 2242, 2939
LweI GCATC 5 cut(s) 187, 1105, 1233, 1734, 1969
MaeI CTAG 4 cut(s) 221, 1286, 2082, 2600
MaeII ACGT 2 cut(s) 356, 2896
MaeIII GTNAC 4 cut(s) 336, 570, 1045, 2133
MbiI CCGCTC 2 cut(s) 256, 701
MflI RGATCY 5 cut(s) 216, 1132, 1675, 2370, 2455
MhlI GDGCHC 7 cut(s) 176, 242, 450, 505, 1415, 1596, 2346
MlsI TGGCCA 1 cut(s) 1056
MluI ACGCGT 1 cut(s) 2543
MluNI TGGCCA 1 cut(s) 1056
MlyI GAGTC 7 cut(s) 72, 126, 182, 651, 1247, 1621, 2797
MmeI TCCRAC 9 cut(s) 205, 255, 663, 861, 1163, 1440, 2146, 2398, 2876
Mox20I TGGCCA 1 cut(s) 1056
Mph1103I ATGCAT 1 cut(s) 1962
MroI TCCGGA 1 cut(s) 714
MscI TGGCCA 1 cut(s) 1056
MseI TTAA 4 cut(s) 2220, 2661, 2714, 2739
MslI CAYNNNNRTG 3 cut(s) 1041, 2303, 2913
Msp20I TGGCCA 1 cut(s) 1056
MspA1I CMGCKG 2 cut(s) 2093, 2708
MspI CCGG 6 cut(s) 137, 235, 243, 292, 432, 715
MspR9I CCNGG 7 cut(s) 137, 433, 662, 896, 976, 1262, 1965
Mva1269I GAATGC 3 cut(s) 1174, 1573, 1883
MvaI CCWGG 5 cut(s) 662, 896, 976, 1262, 1965
MvnI CGCG 1 cut(s) 2545
MwoI GCNNNNNNNGC 8 cut(s) 371, 565, 574, 1772, 1781, 1892, 2616, 2965
NciI CCSGG 2 cut(s) 137, 433
NcoI CCATGG 1 cut(s) 747
NdeI CATATG 1 cut(s) 675
NheI GCTAGC 1 cut(s) 2599
NmuCI GTSAC 2 cut(s) 336, 2133
NsiI ATGCAT 1 cut(s) 1962
NspI RCATGY 1 cut(s) 846
NspV TTCGAA 1 cut(s) 2239
PagI TCATGA 2 cut(s) 963, 2562
PaqCI CACCTGC 2 cut(s) 2362, 2847
PasI CCCWGGG 1 cut(s) 1261
PciSI GCTCTTC 2 cut(s) 933, 2827
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 362
PctI GAATGC 3 cut(s) 1174, 1573, 1883
PfoI TCCNGGA 1 cut(s) 894
PkrI GCNGC 8 cut(s) 387, 520, 1084, 1474, 1572, 1657, 2232, 2929
PleI GAGTC 7 cut(s) 71, 125, 182, 651, 1246, 1621, 2797
PpsI GAGTC 7 cut(s) 71, 125, 182, 651, 1246, 1621, 2797
Ppu21I YACGTR 1 cut(s) 2897
PshBI ATTAAT 1 cut(s) 2220
Psp124BI GAGCTC 4 cut(s) 242, 450, 1596, 2346
Psp6I CCWGG 5 cut(s) 660, 894, 974, 1260, 1963
PspGI CCWGG 5 cut(s) 660, 894, 974, 1260, 1963
PspOMI GGGCCC 1 cut(s) 172
PsrI GAACNNNNNNTAC 2 cut(s) 2035, 2067
PstI CTGCAG 3 cut(s) 815, 2232, 2929
PstNI CAGNNNCTG 2 cut(s) 1399, 2354
PsuI RGATCY 5 cut(s) 216, 1132, 1675, 2370, 2455
PvuII CAGCTG 1 cut(s) 2708
RsaI GTAC 6 cut(s) 829, 1284, 1641, 1647, 2173, 2764
RsaNI GTAC 6 cut(s) 828, 1283, 1640, 1646, 2172, 2763
RseI CAYNNNNRTG 3 cut(s) 1041, 2303, 2913
Rsr2I CGGWCCG 1 cut(s) 429
RsrII CGGWCCG 1 cut(s) 429
SacI GAGCTC 4 cut(s) 242, 450, 1596, 2346
SapI GCTCTTC 2 cut(s) 933, 2827
SaqAI TTAA 4 cut(s) 2220, 2661, 2714, 2739
SatI GCNGC 8 cut(s) 386, 519, 1083, 1473, 1571, 1656, 2231, 2928
ScaI AGTACT 2 cut(s) 1284, 1647
SchI GAGTC 7 cut(s) 72, 126, 182, 651, 1247, 1621, 2797
ScrFI CCNGG 7 cut(s) 137, 433, 662, 896, 976, 1262, 1965
SduI GDGCHC 7 cut(s) 176, 242, 450, 505, 1415, 1596, 2346
SfaNI GCATC 5 cut(s) 187, 1105, 1233, 1734, 1969
SfcI CTRYAG 3 cut(s) 811, 2228, 2925
SfuI TTCGAA 1 cut(s) 2239
SinI GGWCC 4 cut(s) 193, 429, 457, 1517
SmiMI CAYNNNNRTG 3 cut(s) 1041, 2303, 2913
SmlI CTYRAG 2 cut(s) 1595, 2492
SmoI CTYRAG 2 cut(s) 1595, 2492
SpeI ACTAGT 1 cut(s) 1285
SspI AATATT 1 cut(s) 1234
SspMI CTAG 4 cut(s) 221, 1286, 2082, 2600
SstI GAGCTC 4 cut(s) 242, 450, 1596, 2346
StyD4I CCNGG 7 cut(s) 135, 431, 660, 894, 974, 1260, 1963
StyI CCWWGG 4 cut(s) 735, 747, 985, 2022
TaaI ACNGT 9 cut(s) 208, 429, 832, 1211, 1639, 2524, 2629, 2737, 2762
TaiI ACGT 2 cut(s) 359, 2899
TaqI TCGA 5 cut(s) 141, 1039, 1241, 1695, 2239
TaqII GACCGA 1 cut(s) 497
TatI WGTACW 4 cut(s) 1282, 1639, 1645, 2171
Tru1I TTAA 4 cut(s) 2220, 2661, 2714, 2739
Tru9I TTAA 4 cut(s) 2220, 2661, 2714, 2739
TscAI CASTG 6 cut(s) 854, 1231, 1527, 1624, 2634, 2740
TseFI GTSAC 2 cut(s) 336, 2133
TseI GCWGC 8 cut(s) 385, 518, 1082, 1472, 1570, 1655, 2230, 2927
Tsp45I GTSAC 2 cut(s) 336, 2133
TspDTI ATGAA 9 cut(s) 630, 933, 980, 1025, 1935, 2047, 2096, 2430, 2579
TspGWI ACGGA 3 cut(s) 855, 1453, 2570
TspRI CASTG 6 cut(s) 854, 1231, 1527, 1624, 2634, 2740
VpaK11BI GGWCC 4 cut(s) 193, 429, 457, 1517
VspI ATTAAT 1 cut(s) 2220
XapI RAATTY 6 cut(s) 295, 634, 999, 2102, 2663, 2869
XceI RCATGY 1 cut(s) 846
XcmI CCANNNNNNNNNTGG 2 cut(s) 854, 1093
XmiI GTMKAC 1 cut(s) 210
XspI CTAG 4 cut(s) 221, 1286, 2082, 2600
ZraI GACGTC 1 cut(s) 357
ZrmI AGTACT 2 cut(s) 1284, 1647
Zsp2I ATGCAT 1 cut(s) 1962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.