pycom15g17370

Plant family of unknown function (DUF810)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011157
Physical Location & Seq
Forward (+)
12254630 .. 12258397
3768 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g17370.4

Sequence Viewer

Length: 2943 bp
ATGGCTCACCTTCTCAGAGACAGAGTATTCGGAAGCTCCAGACGACACTCCCAAAACGCCAATCCAATTCCTATTCCAATCCAACCCGCCATGCCCGTTCACACAGTTGAAGGCCTCCCCAACCCATTTGGGGAACTGGGTCCGACCCTCTCGGATTCGGAGCTCCGGGAAACAGTCTACGAGATCTTAGTCGGAGCCTGCCGGAGCTCCGGGCCTAAACCGCTCACCTACGTCCCGCAGTCCGAGAAGACCGACCGGAGTGCTCTGACTTCACTGCCGTCGTCGCTTCAGAGGTCGACGTCGTCGGCGGCGAGCCGGTTCAAGAAGGCGCTGGGTATGAAGTCGGCTTCTGGGAGGAGACTCGGTGGCGGGGACTCCGTGAGTCAGGGGAAGAGTAAGTGGACCGGGACGGTTTGGGAGCTTGTGAGGGTTCAGATGAAGGTTTCGGAGCAGACTGATACGAGGGTCCGGAGAGCACTTTTGAGGGTTGCTGCTGGTCAGCTTGGACGGCGAATAGAATGCATGGTATTGCCCCTTGAGCTATTACAGCAGTTCAAGTCTTCTGATTTTCCTACCCAACAAGAATATGAAGCTTGGCAGAGGAGAAACTTGAGGGTTCTCGAAGCCGGACTTCTTTTGTATCCTTATTTGCCCTTAGATAAGAGAGACACTGCTCCTCAACAACTCCGGAAGATTATTCACGGGGCCTTAGATAAACCCATAGAAACTGGAAAGCACACTGAGTCGATGCAAGTTCTCTACAATGTTGTTATGTCTCTTGCTAACAGATCAGTTGATGGTTCTGTTTCTGAGACGTGCCACTGGGCTGATGGGTTTCCGTTCAACCTTAGGCTCTACCAAATGCTACTAGAATCGTGTTTTGACCCGAATGAAGAAACATCTGTTATTGAAGAGCTTGAAGAGGTTTTTGACCTCATAAAGAAGACCTGGGTAGTCCTCGGAATAAACCAAATGCTGCATAATCTTTGTTTCTCGTGGGTTTTATTTCATCGTTATGTTACAACTGGCCAAGTAGATAACGACCTGCTGCTTGCGTCCAGTAACCTGTTGGCGGAAGTTGAGCAAGATGCCTATGGCACAAAAGATCCATCTTACTTGAAGATCTTAAGTTCGACATTGAGTTCGATATTGGGTTGGGCTGAGAAAAGGCTTCTCGCTTACCGAGATAATTTCCACAGTGGCAATATAGAAGCAATGGAAAACAGTCTCTCTCTGGGATTATTATCAGCAAAAATACTGTTTCAAGATATTTCTCATGAATACCGTAGGAAGAGGAAAGAAACTAATGTGGGTTATGACAAGGTTGATGCTTACATAAGATCTTCAATATGCTCCGCTTTTGCGCAGAAACTGGAAAAGGTAGGTTCTAGCAAGCGTTACTCGAAAAGCCAAAACAATCTTCCTGTCCTTACCGCCCTTGCACAAGATGTTAGCAAGCTGGCTTTTAGTGAGAAGGAGATATTTGGTCCAGTATTGAAGAGATGGCACCCTCTTGCAACAGGCATCGCTATGGCTACACTGCATTCTTGCTATGGAAATGAGCTAAAGCAATTTGTGGCGGGTATCAGTGATTTGACGCCGGACACTATACAAGTGCTTAGAGCTGCTGACAAATTGGAAAAAGATCTTGTGCAGATTGCCGTCGAAGATTTAGTGGATAGTGAGGATGGTGGAATGTCGATCATAAGGGAGATGCCTCCTTACGAGGCTGAAGCTGTAATTGCCAATCTGGCAAAGGCTTGGATAAGGACAAGAGTAGACAGGCCGAGGGAATGGGTTGATAGAAATCTGCATCAAGAGGTATGGAATCCAAGGGCAAACAAAGAGCGGTTTGCTCCTTCTGCTGTTGAAGTTTTACGCATTGTAGATGATACGTTGGAAGCATTCTTTATGTTGCCGATACCCATGCATTCTGTCTTGGTTCCAGAACTGTTGATCGGTCTAGACAAATGTATTCAACATTATATCTTGAAGGCCAAATCTGGTTGCGGGACCGGAAACACTTTCATTCCCACTTTACCTGCTTTGACCAGATGCTCAGCAGGGTCAAAATATGGTGTGTTCAAGAAAAAGGAAAGGTTGCATATAAGTCAGAGAAGGAAATCTCAGGTTGGAAGTACAAATGGGGACAGCTCATTTGGGATTCCGCAACTTTGCGTTCGCATTAATACTTTGCAGCTTATTCGAATGGAATTGGGAGTTTTCGAGAAGAGGATAATTTCCCATCTTGGGAGCTCTGAAACCCCTCGGGAGGACAATATCGCAAACGGAGTGGGCAAAATGTTTGAGCTTTCCACATCTGCTTGTGTTGAAGGGATCCAACAACTTTGTGAGGCAACAGCATACAAGGTCGTTTTCCATGACCTAAGTCATGTCCTTTTTGACGGCTTGTATATTGTGGGAGTTTCGTCGTCTAGGATTGAGCCATTTCTTCAGGAGCTTGAGCAATATTTGGAGATTATTTCGTCAACTGTCCATGACAGAGTTAGAACACGTGTCATTACTGATGTAATGAGGGCTTCTATTGACGGGTTCTTGTTGGTTTTGCTAGCTGGAGGACCTTCTCGCACTTTCACGCGGAAAGATTCTGACTTAATCGAGGAGGATTTTAGGTTTCTAACTGATTTATTCTGGTCGAACGGGGATGGACTGCCAGCTGATGTGATTGATAAGTTATCTACCACTATTAAAGGTATCCTCCCTCTGTACGGTATTGATACTGACAGCCTTGTTGAACAGTTCAAACGTGCAACCCTGGAGAACCATGGATCTTCTGCTAAATCTCGGCTTCCAATGCCTCCAACTACAGGCGAGTGGACCTCCAACGAACCAAACACACTTCTACGCGTCTTGTGTTATCGGAATGACGAGACTGCAGCAAAGTTCCTAAAGAAGACTTATAACTTGCCTAAGAAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF810

Protein Analysis

981

Amino Acids

109.81

Weight (kDa)

8.05

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20010 AT2G20010
fragaria_vesca FvH4_1g05840
malus_domestica MD02G1060400.v1.1 MD15G1193900.v1.1
prunus_persica Prupe.7G222400_v2.0.a1 Prupe.7G222400_v2.0.a1
pyrus_communis pycom02g04960 pycom15g17370
rosa_chinensis RchiOBHm_Chr2g0091501 RchiOBHm_Chr2g0091591
rosa_laevigata RLG00000016231
rosa_multiflora Rmu_sc0002407.1_g000007
rosa_roxburghii Rroxscaffold_2G00149980
rosa_rugosa Rorug02G0018300
rosa_samantha Rh2AG063500 Rh2BG062900 Rh2CG064600 Rh2DG063000
rosa_wichuraiana Rw2G005690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2922
AatII GACGTC 1 cut(s) 302
Acc16I TGCGCA 1 cut(s) 1365
Acc36I ACCTGC 2 cut(s) 1053, 2050
AccB1I GGYRCC 1 cut(s) 1506
AccBSI CCGCTC 2 cut(s) 223, 1849
AccI GTMKAC 3 cut(s) 177, 296, 1779
AccII CGCG 2 cut(s) 2599, 2868
AccIII TCCGGA 2 cut(s) 468, 687
AclWI GGATC 4 cut(s) 1100, 2332, 2345, 2797
AcoI YGGCCR 1 cut(s) 1027
AcuI CTGAAG 3 cut(s) 272, 1752, 2438
AcvI CACGTG 1 cut(s) 2516
AcyI GRCGYC 2 cut(s) 299, 1598
AfaI GTAC 2 cut(s) 2140, 2729
AfiI CCNNNNNNNGG 6 cut(s) 130, 1072, 2250, 2272, 2729, 2828
AflII CTTAAG 1 cut(s) 1126
AflIII ACRYGT 3 cut(s) 2513, 2515, 2866
AjiI CACGTC 1 cut(s) 816
AjnI CCWGG 2 cut(s) 947, 2775
AjuI GAANNNNNNNTTGG 2 cut(s) 1404, 1436
Alw21I GWGCWC 5 cut(s) 165, 209, 265, 478, 2258
Alw26I GTCTC 7 cut(s) 12, 352, 660, 780, 806, 1232, 2885
AlwI GGATC 4 cut(s) 1100, 2332, 2345, 2797
AlwNI CAGNNNCTG 1 cut(s) 1372
Ama87I CYCGRG 1 cut(s) 2268
Aor13HI TCCGGA 2 cut(s) 468, 687
AoxI GGCC 6 cut(s) 112, 212, 705, 1027, 1784, 1995
ApeKI GCWGC 6 cut(s) 491, 976, 1048, 1625, 2197, 2897
ArsI GACNNNNNNTTYG 6 cut(s) 11, 43, 1126, 1158, 2141, 2173
AseI ATTAAT 1 cut(s) 2187
AspLEI GCGC 2 cut(s) 331, 1366
AspS9I GGNCC 9 cut(s) 140, 212, 402, 466, 705, 1487, 2013, 2579, 2838
AsuC2I CCSGG 3 cut(s) 167, 211, 406
AsuHPI GGTGA 1 cut(s) 217
AsuII TTCGAA 1 cut(s) 2206
AsuNHI GCTAGC 1 cut(s) 2569
AvaI CYCGRG 1 cut(s) 2268
AvaII GGWCC 7 cut(s) 140, 402, 466, 1487, 2013, 2579, 2838
AxyI CCTNAGG 1 cut(s) 848
BaeI ACNNNNGTAYC 2 cut(s) 450, 483
BalI TGGCCA 1 cut(s) 1029
BamHI GGATCC 1 cut(s) 2337
BanI GGYRCC 1 cut(s) 1506
BanII GRGCYC 3 cut(s) 165, 209, 2258
BauI CACGAG 1 cut(s) 994
BbrPI CACGTG 1 cut(s) 2516
BbsI GAAGAC 4 cut(s) 254, 552, 950, 2921
Bbv12I GWGCWC 5 cut(s) 165, 209, 265, 478, 2258
BbvI GCAGC 6 cut(s) 478, 963, 1035, 1612, 2209, 2909
BccI CCATC 7 cut(s) 791, 824, 1117, 1497, 1682, 2253, 2660
BceAI ACGGC 4 cut(s) 262, 524, 1646, 2422
BcgI CGANNNNNNTGC 8 cut(s) 242, 276, 501, 535, 1909, 1943, 2186, 2220
BciT130I CCWGG 2 cut(s) 949, 2777
BciVI GTATCC 2 cut(s) 651, 2726
BcnI CCSGG 3 cut(s) 167, 211, 406
BcoDI GTCTC 7 cut(s) 12, 352, 660, 780, 806, 1232, 2885
BfaI CTAG 5 cut(s) 869, 1389, 1964, 2436, 2570
BfmI CTRYAG 3 cut(s) 2826, 2895, 2939
BfoI RGCGCY 1 cut(s) 332
BfrI CTTAAG 1 cut(s) 1126
BfuAI ACCTGC 2 cut(s) 1053, 2050
BfuI GTATCC 2 cut(s) 651, 2726
BglI GCCNNNNNGGC 1 cut(s) 1751
BglII AGATCT 4 cut(s) 183, 1122, 1340, 1645
BisI GCNGC 7 cut(s) 309, 492, 977, 1049, 1626, 2198, 2898
BlpI GCTNAGC 1 cut(s) 2059
BlsI GCNGC 7 cut(s) 310, 493, 978, 1050, 1627, 2199, 2899
Bme1390I CCNGG 5 cut(s) 167, 211, 406, 949, 2777
Bme18I GGWCC 7 cut(s) 140, 402, 466, 1487, 2013, 2579, 2838
BmeT110I CYCGRG 1 cut(s) 2268
BmgBI CACGTC 1 cut(s) 816
BmgT120I GGNCC 9 cut(s) 140, 212, 402, 466, 705, 1487, 2013, 2579, 2838
BmiI GGNNCC 8 cut(s) 141, 196, 467, 706, 1508, 1944, 2014, 2339
BmrFI CCNGG 5 cut(s) 167, 211, 406, 949, 2777
BmrI ACTGGG 2 cut(s) 146, 832
BmsI GCATC 7 cut(s) 738, 1078, 1318, 1533, 1704, 1822, 2045
BmtI GCTAGC 1 cut(s) 2573
BmuI ACTGGG 2 cut(s) 146, 832
BoxI GACNNNNGTC 1 cut(s) 2388
BpiI GAAGAC 4 cut(s) 254, 552, 950, 2921
BplI GAGNNNNNCTC 2 cut(s) 2825, 2857
BpmI CTGGAG 3 cut(s) 22, 2595, 2798
Bpu1102I GCTNAGC 1 cut(s) 2059
Bpu14I TTCGAA 1 cut(s) 2206
BpuEI CTTGAG 3 cut(s) 557, 631, 2483
BpuMI CCSGG 3 cut(s) 167, 211, 406
BsaAI YACGTR 1 cut(s) 2516
BsaBI GATNNNNATC 2 cut(s) 1243, 1806
BsaHI GRCGYC 2 cut(s) 299, 1598
BsaJI CCNNGG 7 cut(s) 948, 958, 1787, 1832, 2267, 2775, 2785
BsaWI WCCGGW 4 cut(s) 255, 468, 687, 2015
BsaXI ACNNNNNCTCC 2 cut(s) 349, 379
Bsc4I CCNNNNNNNGG 6 cut(s) 130, 1072, 2250, 2272, 2729, 2828
Bse118I RCCGGY 1 cut(s) 315
Bse1I ACTGG 7 cut(s) 141, 733, 827, 1030, 1059, 1377, 1490
Bse21I CCTNAGG 1 cut(s) 848
Bse3DI GCAATG 1 cut(s) 1221
Bse8I GATNNNNATC 2 cut(s) 1243, 1806
BseAI TCCGGA 2 cut(s) 468, 687
BseBI CCWGG 2 cut(s) 949, 2777
BseDI CCNNGG 7 cut(s) 948, 958, 1787, 1832, 2267, 2775, 2785
BseGI GGATG 2 cut(s) 1693, 2671
BseJI GATNNNNATC 2 cut(s) 1243, 1806
BseLI CCNNNNNNNGG 6 cut(s) 130, 1072, 2250, 2272, 2729, 2828
BseMI GCAATG 1 cut(s) 1221
BseMII CTCAG 6 cut(s) 28, 732, 801, 1152, 2073, 2141
BseNI ACTGG 7 cut(s) 141, 733, 827, 1030, 1059, 1377, 1490
BseRI GAGGAG 4 cut(s) 370, 616, 666, 2636
BseXI GCAGC 6 cut(s) 478, 963, 1035, 1612, 2209, 2909
BseYI CCCAGC 1 cut(s) 331
BsgI GTGCAG 1 cut(s) 1673
Bsh1236I CGCG 2 cut(s) 2599, 2868
Bsh1285I CGRYCG 1 cut(s) 256
BshFI GGCC 6 cut(s) 114, 214, 707, 1029, 1786, 1997
BshNI GGYRCC 1 cut(s) 1506
BsiEI CGRYCG 1 cut(s) 256
BsiHKAI GWGCWC 5 cut(s) 165, 209, 265, 478, 2258
BsiHKCI CYCGRG 1 cut(s) 2268
BslFI GGGAC 5 cut(s) 218, 386, 421, 2026, 2162
BslI CCNNNNNNNGG 6 cut(s) 130, 1072, 2250, 2272, 2729, 2828
BsmAI GTCTC 7 cut(s) 12, 352, 660, 780, 806, 1232, 2885
BsmBI CGTCTC 1 cut(s) 806
BsmFI GGGAC 5 cut(s) 218, 386, 421, 2026, 2162
BsmI GAATGC 4 cut(s) 524, 1543, 1904, 1930
BsnI GGCC 6 cut(s) 114, 214, 707, 1029, 1786, 1997
BsoBI CYCGRG 1 cut(s) 2268
Bsp119I TTCGAA 1 cut(s) 2206
Bsp1286I GDGCHC 5 cut(s) 165, 209, 265, 478, 2258
Bsp13I TCCGGA 2 cut(s) 468, 687
Bsp1720I GCTNAGC 1 cut(s) 2059
Bsp19I CCATGG 1 cut(s) 2785
BspANI GGCC 6 cut(s) 114, 214, 707, 1029, 1786, 1997
BspCNI CTCAG 6 cut(s) 27, 733, 802, 1153, 2072, 2140
BspEI TCCGGA 2 cut(s) 468, 687
BspFNI CGCG 2 cut(s) 2599, 2868
BspHI TCATGA 1 cut(s) 1276
BspLI GGNNCC 8 cut(s) 141, 196, 467, 706, 1508, 1944, 2014, 2339
BspMAI CTGCAG 1 cut(s) 2899
BspMI ACCTGC 2 cut(s) 1053, 2050
BspOI GCTAGC 1 cut(s) 2573
BspPI GGATC 4 cut(s) 1100, 2332, 2345, 2797
BspQI GCTCTTC 1 cut(s) 906
BspT104I TTCGAA 1 cut(s) 2206
BspT107I GGYRCC 1 cut(s) 1506
BspTI CTTAAG 1 cut(s) 1126
BsrBI CCGCTC 2 cut(s) 223, 1849
BsrDI GCAATG 1 cut(s) 1221
BsrFI RCCGGY 1 cut(s) 315
BsrI ACTGG 7 cut(s) 141, 733, 827, 1030, 1059, 1377, 1490
BssAI RCCGGY 1 cut(s) 315
BssECI CCNNGG 7 cut(s) 948, 958, 1787, 1832, 2267, 2775, 2785
BssNI GRCGYC 2 cut(s) 299, 1598
BssSI CACGAG 1 cut(s) 994
BssT1I CCWWGG 2 cut(s) 1832, 2785
Bst2BI CACGAG 1 cut(s) 994
Bst2UI CCWGG 2 cut(s) 949, 2777
Bst6I CTCTTC 6 cut(s) 386, 906, 915, 1286, 1493, 2225
BstACI GRCGYC 2 cut(s) 299, 1598
BstAFI CTTAAG 1 cut(s) 1126
BstBAI YACGTR 1 cut(s) 2516
BstBI TTCGAA 1 cut(s) 2206
BstC8I GCNNGC 8 cut(s) 199, 313, 1053, 1394, 1457, 1461, 2571, 2676
BstDSI CCRYGG 1 cut(s) 2785
BstF5I GGATG 2 cut(s) 1693, 2671
BstFNI CGCG 2 cut(s) 2599, 2868
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 2 cut(s) 331, 1366
BstMAI GTCTC 7 cut(s) 12, 352, 660, 780, 806, 1232, 2885
BstMCI CGRYCG 1 cut(s) 256
BstNI CCWGG 2 cut(s) 949, 2777
BstPAI GACNNNNGTC 1 cut(s) 2388
BstSCI CCNGG 5 cut(s) 165, 209, 404, 947, 2775
BstSFI CTRYAG 3 cut(s) 2826, 2895, 2939
BstUI CGCG 2 cut(s) 2599, 2868
BstV1I GCAGC 6 cut(s) 478, 963, 1035, 1612, 2209, 2909
BstV2I GAAGAC 4 cut(s) 254, 552, 950, 2921
BstX2I RGATCY 7 cut(s) 183, 1105, 1122, 1340, 1645, 2337, 2789
BstYI RGATCY 7 cut(s) 183, 1105, 1122, 1340, 1645, 2337, 2789
Bsu36I CCTNAGG 1 cut(s) 848
BsuI GTATCC 2 cut(s) 651, 2726
BsuRI GGCC 6 cut(s) 114, 214, 707, 1029, 1786, 1997
BtgI CCRYGG 1 cut(s) 2785
BtgZI GCGATG 1 cut(s) 1510
BtrI CACGTC 1 cut(s) 816
BtsCI GGATG 2 cut(s) 1693, 2671
BtsI GCAGTG 3 cut(s) 272, 669, 1538
BtsIMutI CAGTG 7 cut(s) 272, 669, 738, 820, 1204, 1538, 1594
BveI ACCTGC 2 cut(s) 1053, 2050
Cac8I GCNNGC 8 cut(s) 199, 313, 1053, 1394, 1457, 1461, 2571, 2676
CaiI CAGNNNCTG 1 cut(s) 1372
CciI TCATGA 1 cut(s) 1276
CfoI GCGC 2 cut(s) 331, 1366
Cfr10I RCCGGY 1 cut(s) 315
Cfr13I GGNCC 9 cut(s) 140, 212, 402, 466, 705, 1487, 2013, 2579, 2838
CseI GACGC 3 cut(s) 1044, 1606, 2857
Csp6I GTAC 2 cut(s) 2139, 2728
CspCI CAANNNNNGTGG 2 cut(s) 2274, 2309
CviAII CATG 8 cut(s) 91, 523, 1277, 1927, 2381, 2393, 2498, 2786
CviQI GTAC 2 cut(s) 2139, 2728
EaeI YGGCCR 1 cut(s) 1027
Eam1104I CTCTTC 6 cut(s) 386, 906, 915, 1286, 1493, 2225
EarI CTCTTC 6 cut(s) 386, 906, 915, 1286, 1493, 2225
EciI GGCGGA 1 cut(s) 1088
Ecl136II GAGCTC 3 cut(s) 163, 207, 2256
Eco130I CCWWGG 2 cut(s) 1832, 2785
Eco147I AGGCCT 1 cut(s) 114
Eco24I GRGCYC 3 cut(s) 165, 209, 2258
Eco47I GGWCC 7 cut(s) 140, 402, 466, 1487, 2013, 2579, 2838
Eco53kI GAGCTC 3 cut(s) 163, 207, 2256
Eco57I CTGAAG 3 cut(s) 272, 1752, 2438
Eco72I CACGTG 1 cut(s) 2516
Eco81I CCTNAGG 1 cut(s) 848
Eco88I CYCGRG 1 cut(s) 2268
EcoICRI GAGCTC 3 cut(s) 163, 207, 2256
EcoO109I RGGNCCY 2 cut(s) 705, 2579
EcoRII CCWGG 2 cut(s) 947, 2775
EcoT14I CCWWGG 2 cut(s) 1832, 2785
EcoT22I ATGCAT 2 cut(s) 524, 1932
EcoT38I GRGCYC 3 cut(s) 165, 209, 2258
ErhI CCWWGG 2 cut(s) 1832, 2785
Esp3I CGTCTC 1 cut(s) 806
FaeI CATG 8 cut(s) 94, 526, 1280, 1930, 2384, 2396, 2501, 2789
FalI AAGNNNNNCTT 2 cut(s) 615, 647
FaqI GGGAC 5 cut(s) 218, 386, 421, 2026, 2162
FatI CATG 8 cut(s) 90, 522, 1276, 1926, 2380, 2392, 2497, 2785
FauI CCCGC 5 cut(s) 94, 243, 362, 1573, 2003
FblI GTMKAC 3 cut(s) 177, 296, 1779
Fnu4HI GCNGC 7 cut(s) 309, 492, 977, 1049, 1626, 2198, 2898
FokI GGATG 2 cut(s) 1700, 2678
FriOI GRGCYC 3 cut(s) 165, 209, 2258
Fsp4HI GCNGC 7 cut(s) 309, 492, 977, 1049, 1626, 2198, 2898
FspBI CTAG 5 cut(s) 869, 1389, 1964, 2436, 2570
FspI TGCGCA 1 cut(s) 1365
GlaI GCGC 2 cut(s) 330, 1365
GluI GCNGC 7 cut(s) 309, 492, 977, 1049, 1626, 2198, 2898
GsaI CCCAGC 1 cut(s) 335
GsuI CTGGAG 3 cut(s) 22, 2595, 2798
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 6 cut(s) 114, 214, 707, 1029, 1786, 1997
HgaI GACGC 3 cut(s) 1044, 1606, 2857
HhaI GCGC 2 cut(s) 331, 1366
Hin1I GRCGYC 2 cut(s) 299, 1598
Hin1II CATG 8 cut(s) 94, 526, 1280, 1930, 2384, 2396, 2501, 2789
Hin6I GCGC 2 cut(s) 329, 1364
HinP1I GCGC 2 cut(s) 329, 1364
HincII GTYRAC 2 cut(s) 297, 2490
HindII GTYRAC 2 cut(s) 297, 2490
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 9 cut(s) 155, 360, 374, 382, 743, 872, 1828, 2164, 2606
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 7 cut(s) 100, 178, 297, 402, 1780, 2490, 2838
Hpy8I GTNNAC 7 cut(s) 100, 178, 297, 402, 1780, 2490, 2838
Hpy99I CGWCG 7 cut(s) 283, 286, 301, 304, 307, 1667, 2434
HpyCH4IV ACGT 6 cut(s) 231, 299, 815, 1895, 2515, 2767
HpySE526I ACGT 6 cut(s) 231, 299, 815, 1895, 2515, 2767
Hsp92I GRCGYC 2 cut(s) 299, 1598
Hsp92II CATG 8 cut(s) 94, 526, 1280, 1930, 2384, 2396, 2501, 2789
HspAI GCGC 2 cut(s) 329, 1364
Kpn2I TCCGGA 2 cut(s) 468, 687
LguI GCTCTTC 1 cut(s) 906
Lsp1109I GCAGC 6 cut(s) 478, 963, 1035, 1612, 2209, 2909
LweI GCATC 7 cut(s) 738, 1078, 1318, 1533, 1704, 1822, 2045
MaeI CTAG 5 cut(s) 869, 1389, 1964, 2436, 2570
MaeII ACGT 6 cut(s) 231, 299, 815, 1895, 2515, 2767
MaeIII GTNAC 3 cut(s) 1018, 1061, 1397
MbiI CCGCTC 2 cut(s) 223, 1849
MflI RGATCY 7 cut(s) 183, 1105, 1122, 1340, 1645, 2337, 2789
MhlI GDGCHC 5 cut(s) 165, 209, 265, 478, 2258
MlsI TGGCCA 1 cut(s) 1029
MluCI AATT 7 cut(s) 66, 1189, 1571, 1634, 1740, 2213, 2238
MluI ACGCGT 1 cut(s) 2866
MluNI TGGCCA 1 cut(s) 1029
MlyI GAGTC 4 cut(s) 354, 368, 391, 752
MmeI TCCRAC 8 cut(s) 106, 167, 172, 1878, 2113, 2365, 2846, 2868
Mox20I TGGCCA 1 cut(s) 1029
Mph1103I ATGCAT 2 cut(s) 524, 1932
MroI TCCGGA 2 cut(s) 468, 687
MscI TGGCCA 1 cut(s) 1029
MseI TTAA 4 cut(s) 1127, 2187, 2615, 2709
MslI CAYNNNNRTG 2 cut(s) 1014, 1529
Msp20I TGGCCA 1 cut(s) 1029
MspA1I CMGCKG 1 cut(s) 2678
MspCI CTTAAG 1 cut(s) 1126
MspR9I CCNGG 5 cut(s) 167, 211, 406, 949, 2777
Mva1269I GAATGC 4 cut(s) 524, 1543, 1904, 1930
MvaI CCWGG 2 cut(s) 949, 2777
MvnI CGCG 2 cut(s) 2599, 2868
NciI CCSGG 3 cut(s) 167, 211, 406
NcoI CCATGG 1 cut(s) 2785
NheI GCTAGC 1 cut(s) 2569
NlaIII CATG 8 cut(s) 94, 526, 1280, 1930, 2384, 2396, 2501, 2789
NlaIV GGNNCC 8 cut(s) 141, 196, 467, 706, 1508, 1944, 2014, 2339
NmeAIII GCCGAG 2 cut(s) 1812, 2785
NsbI TGCGCA 1 cut(s) 1365
NsiI ATGCAT 2 cut(s) 524, 1932
NspV TTCGAA 1 cut(s) 2206
PagI TCATGA 1 cut(s) 1276
PceI AGGCCT 1 cut(s) 114
PciSI GCTCTTC 1 cut(s) 906
PcsI WCGNNNNNNNCGW 1 cut(s) 308
PctI GAATGC 4 cut(s) 524, 1543, 1904, 1930
PfeI GAWTC 5 cut(s) 155, 872, 1828, 2164, 2606
PflFI GACNNNGTC 1 cut(s) 301
PfoI TCCNGGA 1 cut(s) 165
PkrI GCNGC 7 cut(s) 310, 493, 978, 1050, 1627, 2199, 2899
PleI GAGTC 4 cut(s) 354, 368, 390, 751
PmaCI CACGTG 1 cut(s) 2516
PmlI CACGTG 1 cut(s) 2516
PpsI GAGTC 4 cut(s) 354, 368, 390, 751
Ppu21I YACGTR 1 cut(s) 2516
PpuMI RGGWCCY 1 cut(s) 2579
PshAI GACNNNNGTC 1 cut(s) 2388
PshBI ATTAAT 1 cut(s) 2187
PsiI TTATAA 1 cut(s) 2922
Psp124BI GAGCTC 3 cut(s) 165, 209, 2258
Psp5II RGGWCCY 1 cut(s) 2579
Psp6I CCWGG 2 cut(s) 947, 2775
PspCI CACGTG 1 cut(s) 2516
PspFI CCCAGC 1 cut(s) 331
PspGI CCWGG 2 cut(s) 947, 2775
PspN4I GGNNCC 8 cut(s) 141, 196, 467, 706, 1508, 1944, 2014, 2339
PspPI GGNCC 9 cut(s) 140, 212, 402, 466, 705, 1487, 2013, 2579, 2838
PspPPI RGGWCCY 1 cut(s) 2579
PstI CTGCAG 1 cut(s) 2899
PstNI CAGNNNCTG 1 cut(s) 1372
PsuI RGATCY 7 cut(s) 183, 1105, 1122, 1340, 1645, 2337, 2789
PsyI GACNNNGTC 1 cut(s) 301
PvuII CAGCTG 1 cut(s) 2678
RsaI GTAC 2 cut(s) 2140, 2729
RsaNI GTAC 2 cut(s) 2139, 2728
RseI CAYNNNNRTG 2 cut(s) 1014, 1529
SacI GAGCTC 3 cut(s) 165, 209, 2258
SalI GTCGAC 1 cut(s) 295
SapI GCTCTTC 1 cut(s) 906
SaqAI TTAA 4 cut(s) 1127, 2187, 2615, 2709
SatI GCNGC 7 cut(s) 309, 492, 977, 1049, 1626, 2198, 2898
Sau96I GGNCC 9 cut(s) 140, 212, 402, 466, 705, 1487, 2013, 2579, 2838
SchI GAGTC 4 cut(s) 354, 368, 391, 752
ScrFI CCNGG 5 cut(s) 167, 211, 406, 949, 2777
SduI GDGCHC 5 cut(s) 165, 209, 265, 478, 2258
SfaNI GCATC 7 cut(s) 738, 1078, 1318, 1533, 1704, 1822, 2045
SfcI CTRYAG 3 cut(s) 2826, 2895, 2939
SfuI TTCGAA 1 cut(s) 2206
SinI GGWCC 7 cut(s) 140, 402, 466, 1487, 2013, 2579, 2838
SmiMI CAYNNNNRTG 2 cut(s) 1014, 1529
SmlI CTYRAG 4 cut(s) 536, 610, 1126, 2462
SmoI CTYRAG 4 cut(s) 536, 610, 1126, 2462
Sse9I AATT 7 cut(s) 66, 1189, 1571, 1634, 1740, 2213, 2238
SseBI AGGCCT 1 cut(s) 114
SspI AATATT 1 cut(s) 2471
SspMI CTAG 5 cut(s) 869, 1389, 1964, 2436, 2570
SstI GAGCTC 3 cut(s) 165, 209, 2258
StuI AGGCCT 1 cut(s) 114
StyD4I CCNGG 5 cut(s) 165, 209, 404, 947, 2775
StyI CCWWGG 2 cut(s) 1832, 2785
TaiI ACGT 6 cut(s) 234, 302, 818, 1898, 2518, 2770
TaqII GACCGA 2 cut(s) 266, 1949
TasI AATT 7 cut(s) 66, 1189, 1571, 1634, 1740, 2213, 2238
TatI WGTACW 1 cut(s) 2138
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 5 cut(s) 155, 872, 1828, 2164, 2606
Tru1I TTAA 4 cut(s) 1127, 2187, 2615, 2709
Tru9I TTAA 4 cut(s) 1127, 2187, 2615, 2709
TscAI CASTG 7 cut(s) 279, 676, 745, 827, 1204, 1545, 1594
TseI GCWGC 6 cut(s) 491, 976, 1048, 1625, 2197, 2897
TspDTI ATGAA 7 cut(s) 353, 452, 603, 906, 998, 1293, 2017
TspGWI ACGGA 3 cut(s) 367, 828, 2304
TspRI CASTG 7 cut(s) 279, 676, 745, 827, 1204, 1545, 1594
Tth111I GACNNNGTC 1 cut(s) 301
Vha464I CTTAAG 1 cut(s) 1126
VpaK11BI GGWCC 7 cut(s) 140, 402, 466, 1487, 2013, 2579, 2838
VspI ATTAAT 1 cut(s) 2187
XbaI TCTAGA 1 cut(s) 1963
XcmI CCANNNNNNNNNTGG 2 cut(s) 827, 1066
XmiI GTMKAC 3 cut(s) 177, 296, 1779
XspI CTAG 5 cut(s) 869, 1389, 1964, 2436, 2570
ZraI GACGTC 1 cut(s) 300
Zsp2I ATGCAT 2 cut(s) 524, 1932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.