Rroxscaffold_2G00149980

Plant family of unknown function (DUF810)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011157
Physical Location & Seq
Reverse (-)
87465963 .. 87470529
4567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149980.1

Sequence Viewer

Length: 2964 bp
ATGGCCCAGATTATCAGAGACAGAGTTTTCGGCTCCTTCGGTGGTTCCAGACGACACAGCAGAGTCCCTATTCCTATTCCTATTCCAGAACCCGAACCCGAACTCGTCGTCATGCCCGTTTACCCGGTCGAAGACCTGCCCAACCCGTTTGGGGACTTGGGCCCGCATCTCTCCGACTCGGACCTCCGAGAAACCGTCTACGAGATCCTAGTCGGAGCCTGCCGGAGCTCCGGCCCGAAACCCCTCACCTACACCCCGCAGTCGGAGAAGATCACCGACCGGAATTCCCAGTCTTCTTCTTCTTCTTCTTCTTCCTCGCTGTCGTCACTTCAGAGGTCGCTGACGTCATCGGCGAAGAAGGCTCTGGGTTTGAAGCAAACGGCGTCGTCTAAGACGCTCGGTGGGAGTAGTAAACGGTCCGGGTCGGTTTTTGAGCTCATGCGGGTCCAGATGAGGGTTTCGGAGCAGACCGATACCCGAATCAGGAGAGCCCTTCTCAGAGTTGCTGCTGGTCAGCTCGGACGAAAGATAGAGAGCATGGTTTTGCCTCTGGAGCTGTTACAGCATTTCAGGTCGTTAGATTTTGCGAATCAGCAGGAGTATGAAGCTTGGCAGAGGAGGAATTTGAAGGTTCTTGAAGTGGGACTTCTGGTGTATCCTCATATGCCGTCGGATAGAAAAGACACCGCTCCTCAACAGCTCCGGAAGATTATACGTGGGGCCTTGGAGAAACCCATGGAAACTGGAAAGAACACCGAGACAATGCAAGTTCTTCGCAGTCTTGTTATGTCGCTTGCCTGCAGGTCGTTTGATGGTACTGTTTCCGACACATGCCACTGGGCAGATGGGTTTCCGTTGAACCTCAGGCTCTACCAAAAGCTCCTGGAATCGTGTTTTGACCTGAATGAAGAAACTTCTATTATTGAAGAGCTTGATGAGGTTTTAGAAGTCATGAAGAAGACCTGGGTAGTCCTTGGAATAAACCAAATTCTGCATAATCTGTGTTTCGCATGGGTTTTATTTCATCGATATGTTACAACTGGCCAAGTGGATAATGACCTGCTGTTTGCAGCCAATAACCTCTTGGCGGAAGTTCAGCAAGATGCCAATGCTACAAAAGATCCATCTTATTTGAAGATCGTAAGTTCAACATTGAATGCAATACTGGGTTGGACTGAGAAAAGGCTTCTTGCCTACCGTGATGTGTTCAACACTGGCAATATTGAGTCGATGCAAAACATTGTCTCCCTGGGGGTATTATCAGCAAAAGTACTAGTAGAGGATATCTCTCACGAGTATCGTAGGAAGAGAAAAGAAGTTGATGTGCCGCGTGACAGGGTTGACACCTACATAAGATCATCGATACGCACCGCTTTTGCTCAGAAACTGGAAAAAGTGGGCTCCAACAAGCGCCTATCAAAGAGCCAAAAGAATCGTATTCCTACCTTGTCCGTCCTTGCAACAGAAGTTAGCGAACTGGCCTTTAGTGAGAAAGATATATTTGGTCCAGTGTTGAAGAGATGGCACCCTTTTGCAACGGGTGTTGCTATGGCTACGCTGCATTCTTGCTATGGAGACAAGCTCAAGCAATTTGTGGATGGTATCAGTGAGTTGACTCCTGATACTGTACAAGTACTAAAAGCTGCTGACAAATTGGAGAAAGATCTTGTGCAGATTGCTGTCGAAGATTCAGTAGATAGTGAGGATGGCGGGAAATCCATCATACAGGAGATGCCTCCTTACGAGGCTGAAGCTGTAATGGCTGAGCTTGCGAAGGCTTGGATAAGGACAAGATTGGACAGGCTGAAGGAATGGGTTGACAGAAATCTGCAACAAGAGGTCTGGAATCCACAAGCAAACAAAGAACGCTTTGCTCCTTCTGCCATTGAAGTTTTACGCATTGTAGATGAAACATTGGAAGCATTCTTTATGTTGCCTATACCAATGCATCCCGGCTTGATTCCAGAATTGAAGACTGGTCTTGATAGATGTCTTCAATACTATGTATCCAAGGCCAAGACTGGCTGTGGAACCCGAATTACCTTCATTCCCACTATGCCTGCTTTGACTAGATGTTCAGCGGGATCAAAATTTCATGGTGTATTCAAGAAGAAGGAAAGGTCACATATAAACCAGAGGAGGAAATCCCAGGTTGGAAGTACAAATGGGGATGGTTCGTTTGGGATTCCGCAACTTTGTGTTCGCATTAATACTCTGCAGCGTATTCGAATGGAGTTGGGAGTTTTTGAGAAGAGGATTGTTGCTCATCTTGGGAACTTGGAATCCACTCAAAAGGGAGATTTTGCGAATGGAATGGGCAAAATGTTTGAGCTCTCAGCATCTGCTTGTTTAGAAGGGATCCAACAACTTTGTGAGGCAACAGCTTACAAGGTTGTTTTCCATGAACTAAGTCATGTTCTTTGGGATGGCTTGTATAATGTGGATGCTCCCTCATGTAGAATTGAGCCTTTTCTTCAGGAACTCGAGCAATTTTTGGAGATTATCTCGTCAACAGTTCACGACAGAGTTAGAACGCGTCTTATTACGGATGTCATGAAAGCTTCTTTTGACGGGTTCTTGTTGGTTTTGCTAGCTGGAGGCCCTTCCTGCAGTTTCACACTGAGAGATTCTAACATAATAGAGGAGGATTTCAAATTTCTGACTGATTTGTTCTGGTCAAACGGGGATGGATTACCAGCTGATTTAATAGCAAAGTTGTCAACCACTGTTAAGGACATCCTTCCTCTCTACCGTACTGAGACTGATAGCCTTACTGAAAAGTTCAAGCGCGCGACTCTGGAGAACTATGGCTCTTCTGCGAAGTCCCACCTGCCATTGCCTCCAACTTCCGATGAGTGGAATTCCAATGACCCAAATACACTTCTACGTGTCTTATGTCATAGGAATGATGAGACTGCAGCCAAGTTTCTGAAGAAGACTTACAATTTGCCTACAAAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF810

Protein Analysis

987

Amino Acids

110.92

Weight (kDa)

8.02

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PATROL1 PF25761 588 - 986 5.2e-165 PATROL1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20010 AT2G20010
fragaria_vesca FvH4_1g05840
malus_domestica MD02G1060400.v1.1 MD15G1193900.v1.1
prunus_persica Prupe.7G222400_v2.0.a1 Prupe.7G222400_v2.0.a1
pyrus_communis pycom02g04960 pycom15g17370
rosa_chinensis RchiOBHm_Chr2g0091501 RchiOBHm_Chr2g0091591
rosa_laevigata RLG00000016231
rosa_multiflora Rmu_sc0002407.1_g000007
rosa_roxburghii Rroxscaffold_2G00149980
rosa_rugosa Rorug02G0018300
rosa_samantha Rh2AG063500 Rh2BG062900 Rh2CG064600 Rh2DG063000
rosa_wichuraiana Rw2G005690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2838
AatII GACGTC 1 cut(s) 347
Acc36I ACCTGC 4 cut(s) 144, 792, 1068, 2838
AccB1I GGYRCC 1 cut(s) 1524
AccBSI CCGCTC 1 cut(s) 689
AccI GTMKAC 1 cut(s) 198
AccII CGCG 4 cut(s) 1330, 2536, 2790, 2792
AccIII TCCGGA 1 cut(s) 702
AclWI GGATC 5 cut(s) 199, 1115, 2092, 2353, 2366
AcoI YGGCCR 1 cut(s) 1042
AcsI RAATTY 6 cut(s) 283, 622, 987, 2090, 2654, 2860
AcuI CTGAAG 5 cut(s) 314, 1770, 1826, 2459, 2951
AcyI GRCGYC 2 cut(s) 344, 383
AfaI GTAC 6 cut(s) 817, 1272, 1629, 1635, 2161, 2755
AfiI CCNNNNNNNGG 6 cut(s) 151, 262, 454, 483, 1087, 2856
AflIII ACRYGT 2 cut(s) 2534, 2887
AhlI ACTAGT 1 cut(s) 1273
AjnI CCWGG 4 cut(s) 882, 962, 1248, 2148
AloI GAACNNNNNNTCC 2 cut(s) 2267, 2299
Alw21I GWGCWC 3 cut(s) 230, 438, 2334
Alw26I GTCTC 6 cut(s) 12, 752, 1249, 1569, 2753, 2906
AlwI GGATC 5 cut(s) 199, 1115, 2092, 2353, 2366
AlwNI CAGNNNCTG 2 cut(s) 1387, 2342
Ama87I CYCGRG 1 cut(s) 2483
Aor13HI TCCGGA 1 cut(s) 702
AoxI GGCC 8 cut(s) 3, 160, 232, 720, 1042, 1479, 2013, 2599
ApaI GGGCCC 1 cut(s) 164
ApeKI GCWGC 6 cut(s) 506, 1070, 1558, 1643, 2218, 2918
ApoI RAATTY 6 cut(s) 283, 622, 987, 2090, 2654, 2860
ArsI GACNNNNNNTTYG 8 cut(s) 11, 43, 93, 125, 1049, 1081, 2929, 2961
AseI ATTAAT 1 cut(s) 2208
AspLEI GCGC 3 cut(s) 1413, 2790, 2792
AsuC2I CCSGG 3 cut(s) 125, 421, 1953
AsuHPI GGTGA 2 cut(s) 238, 265
AsuII TTCGAA 1 cut(s) 2227
AsuNHI GCTAGC 1 cut(s) 2590
AvaI CYCGRG 1 cut(s) 2483
AvaII GGWCC 4 cut(s) 181, 417, 445, 1505
AxyI CCTNAGG 1 cut(s) 863
BaeGI GKGCMC 1 cut(s) 164
BalI TGGCCA 1 cut(s) 1044
BamHI GGATCC 1 cut(s) 2358
BanI GGYRCC 1 cut(s) 1524
BanII GRGCYC 6 cut(s) 164, 230, 438, 493, 1403, 2334
BarI GAAGNNNNNNTAC 2 cut(s) 2924, 2956
BauI CACGAG 1 cut(s) 1292
BbsI GAAGAC 6 cut(s) 138, 285, 965, 1979, 1985, 2942
Bbv12I GWGCWC 3 cut(s) 230, 438, 2334
BbvI GCAGC 6 cut(s) 493, 1082, 1545, 1630, 2230, 2930
BceAI ACGGC 2 cut(s) 396, 652
BcgI CGANNNNNNTGC 4 cut(s) 755, 789, 2207, 2241
BciT130I CCWGG 4 cut(s) 884, 964, 1250, 2150
BciVI GTATCC 2 cut(s) 666, 2017
BcnI CCSGG 3 cut(s) 125, 421, 1953
BcoDI GTCTC 6 cut(s) 12, 752, 1249, 1569, 2753, 2906
BcuI ACTAGT 1 cut(s) 1273
BfaI CTAG 4 cut(s) 209, 1274, 2070, 2591
BfmI CTRYAG 4 cut(s) 799, 2216, 2608, 2916
BfoI RGCGCY 1 cut(s) 1414
BfuAI ACCTGC 4 cut(s) 144, 792, 1068, 2838
BfuI GTATCC 2 cut(s) 666, 2017
BglII AGATCT 1 cut(s) 1663
BisI GCNGC 7 cut(s) 507, 1071, 1328, 1559, 1644, 2219, 2919
BlpI GCTNAGC 1 cut(s) 1764
BlsI GCNGC 7 cut(s) 508, 1072, 1329, 1560, 1645, 2220, 2920
BmcAI AGTACT 2 cut(s) 1272, 1635
Bme1390I CCNGG 7 cut(s) 125, 421, 884, 964, 1250, 1953, 2150
Bme18I GGWCC 4 cut(s) 181, 417, 445, 1505
BmeT110I CYCGRG 1 cut(s) 2483
BmrFI CCNGG 7 cut(s) 125, 421, 884, 964, 1250, 1953, 2150
BmrI ACTGGG 3 cut(s) 283, 847, 1175
BmsI GCATC 7 cut(s) 175, 1093, 1221, 1722, 1957, 2348, 2434
BmtI GCTAGC 1 cut(s) 2594
BmuI ACTGGG 3 cut(s) 283, 847, 1175
BpiI GAAGAC 6 cut(s) 138, 285, 965, 1979, 1985, 2942
BplI GAGNNNNNCTC 8 cut(s) 480, 512, 1271, 1303, 1566, 1598, 2489, 2521
BpmI CTGGAG 3 cut(s) 572, 2616, 2819
Bpu1102I GCTNAGC 1 cut(s) 1764
Bpu14I TTCGAA 1 cut(s) 2227
BpuEI CTTGAG 1 cut(s) 1568
BpuMI CCSGG 3 cut(s) 125, 421, 1953
Bsa29I ATCGAT 2 cut(s) 1027, 1361
BsaAI YACGTR 2 cut(s) 716, 2888
BsaHI GRCGYC 2 cut(s) 344, 383
BsaJI CCNNGG 8 cut(s) 723, 735, 963, 973, 1248, 1249, 2010, 2148
BsaWI WCCGGW 2 cut(s) 279, 702
BsaXI ACNNNNNCTCC 2 cut(s) 1229, 1259
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 262, 454, 483, 1087, 2856
Bse21I CCTNAGG 1 cut(s) 863
Bse3DI GCAATG 1 cut(s) 2834
BseAI TCCGGA 1 cut(s) 702
BseBI CCWGG 4 cut(s) 884, 964, 1250, 2150
BseCI ATCGAT 2 cut(s) 1027, 1361
BseDI CCNNGG 8 cut(s) 723, 735, 963, 973, 1248, 1249, 2010, 2148
BseGI GGATG 9 cut(s) 1603, 1711, 1948, 2176, 2431, 2449, 2554, 2692, 2736
BseLI CCNNNNNNNGG 6 cut(s) 151, 262, 454, 483, 1087, 2856
BseMI GCAATG 1 cut(s) 2834
BseMII CTCAG 8 cut(s) 511, 877, 1167, 1394, 1755, 2349, 2612, 2748
BsePI GCGCGC 1 cut(s) 2788
BseRI GAGGAG 4 cut(s) 631, 681, 2152, 2657
BseSI GKGCMC 1 cut(s) 164
BseXI GCAGC 6 cut(s) 493, 1082, 1545, 1630, 2230, 2930
BsgI GTGCAG 1 cut(s) 1691
Bsh1236I CGCG 4 cut(s) 1330, 2536, 2790, 2792
Bsh1285I CGRYCG 2 cut(s) 129, 280
BshFI GGCC 8 cut(s) 5, 162, 234, 722, 1044, 1481, 2015, 2601
BshNI GGYRCC 1 cut(s) 1524
BshVI ATCGAT 2 cut(s) 1027, 1361
BsiEI CGRYCG 2 cut(s) 129, 280
BsiHKAI GWGCWC 3 cut(s) 230, 438, 2334
BsiHKCI CYCGRG 1 cut(s) 2483
BsiSI CCGG 7 cut(s) 125, 223, 231, 280, 420, 703, 1953
BslFI GGGAC 4 cut(s) 50, 167, 657, 2809
BslI CCNNNNNNNGG 6 cut(s) 151, 262, 454, 483, 1087, 2856
BsmAI GTCTC 6 cut(s) 12, 752, 1249, 1569, 2753, 2906
BsmFI GGGAC 4 cut(s) 50, 167, 657, 2809
BsmI GAATGC 3 cut(s) 1162, 1561, 1922
BsnI GGCC 8 cut(s) 5, 162, 234, 722, 1044, 1481, 2015, 2601
BsoBI CYCGRG 1 cut(s) 2483
Bsp119I TTCGAA 1 cut(s) 2227
Bsp120I GGGCCC 1 cut(s) 160
Bsp1286I GDGCHC 6 cut(s) 164, 230, 438, 493, 1403, 2334
Bsp13I TCCGGA 1 cut(s) 702
Bsp1407I TGTACA 1 cut(s) 1627
Bsp143I GATC 8 cut(s) 204, 270, 1120, 1137, 1355, 1663, 2084, 2358
Bsp1720I GCTNAGC 1 cut(s) 1764
Bsp19I CCATGG 1 cut(s) 735
BspANI GGCC 8 cut(s) 5, 162, 234, 722, 1044, 1481, 2015, 2601
BspCNI CTCAG 8 cut(s) 510, 876, 1168, 1393, 1756, 2348, 2613, 2749
BspDI ATCGAT 2 cut(s) 1027, 1361
BspEI TCCGGA 1 cut(s) 702
BspFNI CGCG 4 cut(s) 1330, 2536, 2790, 2792
BspHI TCATGA 2 cut(s) 951, 2553
BspMAI CTGCAG 4 cut(s) 803, 2220, 2612, 2920
BspMI ACCTGC 4 cut(s) 144, 792, 1068, 2838
BspOI GCTAGC 1 cut(s) 2594
BspPI GGATC 5 cut(s) 199, 1115, 2092, 2353, 2366
BspQI GCTCTTC 2 cut(s) 921, 2818
BspT104I TTCGAA 1 cut(s) 2227
BspT107I GGYRCC 1 cut(s) 1524
BsrBI CCGCTC 1 cut(s) 689
BsrDI GCAATG 1 cut(s) 2834
BsrGI TGTACA 1 cut(s) 1627
BssECI CCNNGG 8 cut(s) 723, 735, 963, 973, 1248, 1249, 2010, 2148
BssHII GCGCGC 1 cut(s) 2788
BssMI GATC 8 cut(s) 204, 270, 1120, 1137, 1355, 1663, 2084, 2358
BssNI GRCGYC 2 cut(s) 344, 383
BssSI CACGAG 1 cut(s) 1292
BssT1I CCWWGG 4 cut(s) 723, 735, 973, 2010
Bst2BI CACGAG 1 cut(s) 1292
Bst2UI CCWGG 4 cut(s) 884, 964, 1250, 2150
Bst4CI ACNGT 8 cut(s) 196, 417, 820, 1199, 1627, 2515, 2728, 2753
Bst6I CTCTTC 5 cut(s) 921, 1301, 1511, 2246, 2818
BstACI GRCGYC 2 cut(s) 344, 383
BstAUI TGTACA 1 cut(s) 1627
BstBAI YACGTR 2 cut(s) 716, 2888
BstBI TTCGAA 1 cut(s) 2227
BstC8I GCNNGC 8 cut(s) 164, 220, 795, 799, 1770, 2061, 2592, 2790
BstDSI CCRYGG 1 cut(s) 735
BstF5I GGATG 9 cut(s) 1603, 1711, 1948, 2176, 2431, 2449, 2554, 2692, 2736
BstFNI CGCG 4 cut(s) 1330, 2536, 2790, 2792
BstH2I RGCGCY 1 cut(s) 1414
BstHHI GCGC 3 cut(s) 1413, 2790, 2792
BstKTI GATC 8 cut(s) 207, 273, 1123, 1140, 1358, 1666, 2087, 2361
BstMAI GTCTC 6 cut(s) 12, 752, 1249, 1569, 2753, 2906
BstMBI GATC 8 cut(s) 204, 270, 1120, 1137, 1355, 1663, 2084, 2358
BstMCI CGRYCG 2 cut(s) 129, 280
BstMWI GCNNNNNNNGC 8 cut(s) 359, 553, 562, 1760, 1769, 1880, 2607, 2956
BstNI CCWGG 4 cut(s) 884, 964, 1250, 2150
BstNSI RCATGY 1 cut(s) 834
BstSCI CCNGG 7 cut(s) 123, 419, 882, 962, 1248, 1951, 2148
BstSFI CTRYAG 4 cut(s) 799, 2216, 2608, 2916
BstSLI GKGCMC 1 cut(s) 164
BstUI CGCG 4 cut(s) 1330, 2536, 2790, 2792
BstV1I GCAGC 6 cut(s) 493, 1082, 1545, 1630, 2230, 2930
BstV2I GAAGAC 6 cut(s) 138, 285, 965, 1979, 1985, 2942
BstX2I RGATCY 4 cut(s) 204, 1120, 1663, 2358
BstYI RGATCY 4 cut(s) 204, 1120, 1663, 2358
Bsu15I ATCGAT 2 cut(s) 1027, 1361
Bsu36I CCTNAGG 1 cut(s) 863
BsuI GTATCC 2 cut(s) 666, 2017
BsuRI GGCC 8 cut(s) 5, 162, 234, 722, 1044, 1481, 2015, 2601
BsuTUI ATCGAT 2 cut(s) 1027, 1361
BtgI CCRYGG 1 cut(s) 735
BtsCI GGATG 9 cut(s) 1603, 1711, 1948, 2176, 2431, 2449, 2554, 2692, 2736
BtsIMutI CAGTG 6 cut(s) 835, 1212, 1515, 1612, 2618, 2724
BveI ACCTGC 4 cut(s) 144, 792, 1068, 2838
Cac8I GCNNGC 8 cut(s) 164, 220, 795, 799, 1770, 2061, 2592, 2790
CaiI CAGNNNCTG 2 cut(s) 1387, 2342
CciI TCATGA 2 cut(s) 951, 2553
CfoI GCGC 3 cut(s) 1413, 2790, 2792
ClaI ATCGAT 2 cut(s) 1027, 1361
CpoI CGGWCCG 1 cut(s) 417
CseI GACGC 3 cut(s) 372, 403, 2525
Csp6I GTAC 6 cut(s) 816, 1271, 1628, 1634, 2160, 2754
CspI CGGWCCG 1 cut(s) 417
CviQI GTAC 6 cut(s) 816, 1271, 1628, 1634, 2160, 2754
DpnI GATC 8 cut(s) 206, 272, 1122, 1139, 1357, 1665, 2086, 2360
DpnII GATC 8 cut(s) 204, 270, 1120, 1137, 1355, 1663, 2084, 2358
EaeI YGGCCR 1 cut(s) 1042
Eam1104I CTCTTC 5 cut(s) 921, 1301, 1511, 2246, 2818
EarI CTCTTC 5 cut(s) 921, 1301, 1511, 2246, 2818
EciI GGCGGA 1 cut(s) 1103
Ecl136II GAGCTC 3 cut(s) 228, 436, 2332
Eco130I CCWWGG 4 cut(s) 723, 735, 973, 2010
Eco24I GRGCYC 6 cut(s) 164, 230, 438, 493, 1403, 2334
Eco32I GATATC 1 cut(s) 1285
Eco47I GGWCC 4 cut(s) 181, 417, 445, 1505
Eco53kI GAGCTC 3 cut(s) 228, 436, 2332
Eco57I CTGAAG 5 cut(s) 314, 1770, 1826, 2459, 2951
Eco81I CCTNAGG 1 cut(s) 863
Eco88I CYCGRG 1 cut(s) 2483
EcoICRI GAGCTC 3 cut(s) 228, 436, 2332
EcoO109I RGGNCCY 2 cut(s) 720, 2600
EcoRI GAATTC 2 cut(s) 283, 2860
EcoRII CCWGG 4 cut(s) 882, 962, 1248, 2148
EcoRV GATATC 1 cut(s) 1285
EcoT14I CCWWGG 4 cut(s) 723, 735, 973, 2010
EcoT22I ATGCAT 1 cut(s) 1950
EcoT38I GRGCYC 6 cut(s) 164, 230, 438, 493, 1403, 2334
ErhI CCWWGG 4 cut(s) 723, 735, 973, 2010
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 4 cut(s) 50, 167, 657, 2809
FauI CCCGC 5 cut(s) 171, 264, 435, 1703, 2074
FauNDI CATATG 1 cut(s) 663
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 7 cut(s) 507, 1071, 1328, 1559, 1644, 2219, 2919
FokI GGATG 9 cut(s) 1610, 1718, 1935, 2183, 2438, 2456, 2561, 2699, 2723
FriOI GRGCYC 6 cut(s) 164, 230, 438, 493, 1403, 2334
Fsp4HI GCNGC 7 cut(s) 507, 1071, 1328, 1559, 1644, 2219, 2919
FspBI CTAG 4 cut(s) 209, 1274, 2070, 2591
GlaI GCGC 3 cut(s) 1412, 2789, 2791
GluI GCNGC 7 cut(s) 507, 1071, 1328, 1559, 1644, 2219, 2919
GsuI CTGGAG 3 cut(s) 572, 2616, 2819
HaeII RGCGCY 1 cut(s) 1414
HaeIII GGCC 8 cut(s) 5, 162, 234, 722, 1044, 1481, 2015, 2601
HapII CCGG 7 cut(s) 125, 223, 231, 280, 420, 703, 1953
HgaI GACGC 3 cut(s) 372, 403, 2525
HhaI GCGC 3 cut(s) 1413, 2790, 2792
Hin1I GRCGYC 2 cut(s) 344, 383
Hin6I GCGC 3 cut(s) 1411, 2788, 2790
HinP1I GCGC 3 cut(s) 1411, 2788, 2790
HincII GTYRAC 5 cut(s) 1342, 1614, 1819, 2511, 2721
HindII GTYRAC 5 cut(s) 1342, 1614, 1819, 2511, 2721
HindIII AAGCTT 2 cut(s) 606, 2559
HpaII CCGG 7 cut(s) 125, 223, 231, 280, 420, 703, 1953
HphI GGTGA 2 cut(s) 238, 265
Hpy166II GTNNAC 9 cut(s) 121, 199, 413, 1342, 1614, 1819, 2511, 2518, 2721
Hpy8I GTNNAC 9 cut(s) 121, 199, 413, 1342, 1614, 1819, 2511, 2518, 2721
Hpy99I CGWCG 3 cut(s) 110, 388, 673
HpyCH4III ACNGT 8 cut(s) 196, 417, 820, 1199, 1627, 2515, 2728, 2753
HpyCH4IV ACGT 3 cut(s) 344, 715, 2887
HpyF10VI GCNNNNNNNGC 8 cut(s) 359, 553, 562, 1760, 1769, 1880, 2607, 2956
HpySE526I ACGT 3 cut(s) 344, 715, 2887
Hsp92I GRCGYC 2 cut(s) 344, 383
HspAI GCGC 3 cut(s) 1411, 2788, 2790
Kpn2I TCCGGA 1 cut(s) 702
Kzo9I GATC 8 cut(s) 204, 270, 1120, 1137, 1355, 1663, 2084, 2358
LguI GCTCTTC 2 cut(s) 921, 2818
Lsp1109I GCAGC 6 cut(s) 493, 1082, 1545, 1630, 2230, 2930
LweI GCATC 7 cut(s) 175, 1093, 1221, 1722, 1957, 2348, 2434
MaeI CTAG 4 cut(s) 209, 1274, 2070, 2591
MaeII ACGT 3 cut(s) 344, 715, 2887
MaeIII GTNAC 5 cut(s) 324, 558, 1033, 1331, 2121
MalI GATC 8 cut(s) 206, 272, 1122, 1139, 1357, 1665, 2086, 2360
MbiI CCGCTC 1 cut(s) 689
MboI GATC 8 cut(s) 204, 270, 1120, 1137, 1355, 1663, 2084, 2358
MflI RGATCY 4 cut(s) 204, 1120, 1663, 2358
MhlI GDGCHC 6 cut(s) 164, 230, 438, 493, 1403, 2334
MlsI TGGCCA 1 cut(s) 1044
MluI ACGCGT 1 cut(s) 2534
MluNI TGGCCA 1 cut(s) 1044
MlyI GAGTC 5 cut(s) 72, 170, 1235, 1609, 2788
Mox20I TGGCCA 1 cut(s) 1044
Mph1103I ATGCAT 1 cut(s) 1950
MroI TCCGGA 1 cut(s) 702
MscI TGGCCA 1 cut(s) 1044
MseI TTAA 3 cut(s) 2208, 2705, 2730
MslI CAYNNNNRTG 2 cut(s) 1029, 2904
Msp20I TGGCCA 1 cut(s) 1044
MspA1I CMGCKG 2 cut(s) 2081, 2699
MspI CCGG 7 cut(s) 125, 223, 231, 280, 420, 703, 1953
MspR9I CCNGG 7 cut(s) 125, 421, 884, 964, 1250, 1953, 2150
Mva1269I GAATGC 3 cut(s) 1162, 1561, 1922
MvaI CCWGG 4 cut(s) 884, 964, 1250, 2150
MvnI CGCG 4 cut(s) 1330, 2536, 2790, 2792
MwoI GCNNNNNNNGC 8 cut(s) 359, 553, 562, 1760, 1769, 1880, 2607, 2956
NciI CCSGG 3 cut(s) 125, 421, 1953
NcoI CCATGG 1 cut(s) 735
NdeI CATATG 1 cut(s) 663
NdeII GATC 8 cut(s) 204, 270, 1120, 1137, 1355, 1663, 2084, 2358
NheI GCTAGC 1 cut(s) 2590
NmuCI GTSAC 3 cut(s) 324, 1331, 2121
NsiI ATGCAT 1 cut(s) 1950
NspI RCATGY 1 cut(s) 834
NspV TTCGAA 1 cut(s) 2227
PaeR7I CTCGAG 1 cut(s) 2483
PagI TCATGA 2 cut(s) 951, 2553
PaqCI CACCTGC 1 cut(s) 2838
PasI CCCWGGG 1 cut(s) 1249
PauI GCGCGC 1 cut(s) 2788
PciSI GCTCTTC 2 cut(s) 921, 2818
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 350
PctI GAATGC 3 cut(s) 1162, 1561, 1922
PfoI TCCNGGA 1 cut(s) 882
PkrI GCNGC 7 cut(s) 508, 1072, 1329, 1560, 1645, 2220, 2920
PleI GAGTC 5 cut(s) 71, 170, 1234, 1609, 2788
PpsI GAGTC 5 cut(s) 71, 170, 1234, 1609, 2788
Ppu21I YACGTR 2 cut(s) 716, 2888
PshBI ATTAAT 1 cut(s) 2208
Psp124BI GAGCTC 3 cut(s) 230, 438, 2334
Psp6I CCWGG 4 cut(s) 882, 962, 1248, 2148
PspGI CCWGG 4 cut(s) 882, 962, 1248, 2148
PspOMI GGGCCC 1 cut(s) 160
PspXI VCTCGAGB 1 cut(s) 2483
PsrI GAACNNNNNNTAC 2 cut(s) 2023, 2055
PstI CTGCAG 4 cut(s) 803, 2220, 2612, 2920
PstNI CAGNNNCTG 2 cut(s) 1387, 2342
PsuI RGATCY 4 cut(s) 204, 1120, 1663, 2358
PteI GCGCGC 1 cut(s) 2788
PvuII CAGCTG 1 cut(s) 2699
RsaI GTAC 6 cut(s) 817, 1272, 1629, 1635, 2161, 2755
RsaNI GTAC 6 cut(s) 816, 1271, 1628, 1634, 2160, 2754
RseI CAYNNNNRTG 2 cut(s) 1029, 2904
Rsr2I CGGWCCG 1 cut(s) 417
RsrII CGGWCCG 1 cut(s) 417
SacI GAGCTC 3 cut(s) 230, 438, 2334
SapI GCTCTTC 2 cut(s) 921, 2818
SaqAI TTAA 3 cut(s) 2208, 2705, 2730
SatI GCNGC 7 cut(s) 507, 1071, 1328, 1559, 1644, 2219, 2919
Sau3AI GATC 8 cut(s) 204, 270, 1120, 1137, 1355, 1663, 2084, 2358
SbfI CCTGCAGG 1 cut(s) 803
ScaI AGTACT 2 cut(s) 1272, 1635
SchI GAGTC 5 cut(s) 72, 170, 1235, 1609, 2788
ScrFI CCNGG 7 cut(s) 125, 421, 884, 964, 1250, 1953, 2150
SdaI CCTGCAGG 1 cut(s) 803
SduI GDGCHC 6 cut(s) 164, 230, 438, 493, 1403, 2334
SfaNI GCATC 7 cut(s) 175, 1093, 1221, 1722, 1957, 2348, 2434
SfcI CTRYAG 4 cut(s) 799, 2216, 2608, 2916
Sfr274I CTCGAG 1 cut(s) 2483
SfuI TTCGAA 1 cut(s) 2227
SinI GGWCC 4 cut(s) 181, 417, 445, 1505
SlaI CTCGAG 1 cut(s) 2483
SmiMI CAYNNNNRTG 2 cut(s) 1029, 2904
SmlI CTYRAG 2 cut(s) 1583, 2483
SmoI CTYRAG 2 cut(s) 1583, 2483
SpeI ACTAGT 1 cut(s) 1273
Sse8387I CCTGCAGG 1 cut(s) 803
SspI AATATT 1 cut(s) 1222
SspMI CTAG 4 cut(s) 209, 1274, 2070, 2591
SstI GAGCTC 3 cut(s) 230, 438, 2334
StyD4I CCNGG 7 cut(s) 123, 419, 882, 962, 1248, 1951, 2148
StyI CCWWGG 4 cut(s) 723, 735, 973, 2010
TaaI ACNGT 8 cut(s) 196, 417, 820, 1199, 1627, 2515, 2728, 2753
TaiI ACGT 3 cut(s) 347, 718, 2890
TaqI TCGA 7 cut(s) 129, 1027, 1229, 1361, 1683, 2227, 2484
TaqII GACCGA 1 cut(s) 485
TatI WGTACW 4 cut(s) 1270, 1627, 1633, 2159
TauI GCSGC 1 cut(s) 1330
Tru1I TTAA 3 cut(s) 2208, 2705, 2730
Tru9I TTAA 3 cut(s) 2208, 2705, 2730
TscAI CASTG 6 cut(s) 842, 1219, 1515, 1612, 2625, 2731
TseFI GTSAC 3 cut(s) 324, 1331, 2121
TseI GCWGC 6 cut(s) 506, 1070, 1558, 1643, 2218, 2918
Tsp45I GTSAC 3 cut(s) 324, 1331, 2121
TspDTI ATGAA 9 cut(s) 618, 921, 968, 1013, 1923, 2035, 2084, 2418, 2570
TspGWI ACGGA 3 cut(s) 843, 1441, 2561
TspRI CASTG 6 cut(s) 842, 1219, 1515, 1612, 2625, 2731
VpaK11BI GGWCC 4 cut(s) 181, 417, 445, 1505
VspI ATTAAT 1 cut(s) 2208
XapI RAATTY 6 cut(s) 283, 622, 987, 2090, 2654, 2860
XceI RCATGY 1 cut(s) 834
XcmI CCANNNNNNNNNTGG 2 cut(s) 842, 1081
XhoI CTCGAG 1 cut(s) 2483
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 4 cut(s) 209, 1274, 2070, 2591
ZraI GACGTC 1 cut(s) 345
ZrmI AGTACT 2 cut(s) 1272, 1635
Zsp2I ATGCAT 1 cut(s) 1950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.