Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010515

Physical Location & Seq

Genomic Coordinates Forward (+)

6421791 .. 6424140

Gene Structure 2350 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1082000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1455 bp

ATGGTGGATCCTGACGTTGGTGGCGGTTCTGGCGGGGGAGACGGCGGTGGGAGGCCTTCCCTGCCATTTCCGTCCAAAACACAGTTGACAGAACCCAGCAAAAATCTAGAAGAAGCTGAGAACGAGCTTGATCGAGCCAAAGAAGAAGTGACTGAACTGAAAAAAGAGGATAAGGCGGTGACTTTTTCCGAAGGCAGAAACTTTGACCGGTCATTCTCTTCCTCTTCCTCCACCGCTCCGCCAAACCCAGCGGCCGAATTGCTAAAGGTGAAAGAAGAAACTGTAGATGTTGTGGTTGTGGATAATGACTTAGAGGGTGGAGATCATTGCAAGACCATCAACGGCGGCGGTGGCGGTGGTGATTTTGGGTCGCTTTCTTCGTCTTCTTCCATGGAGCTGCCTAAACCCATGGAGGGTTTGCGCGAGGCGGGCCCACCACCGTTCTTGAACAAGACGTTTCAGATGGTGGACGACCCGGAAACCGATTCGATCGTTTCGTGGAGTGATGCTCAGCAGAGCTTCATTGTTTGGGACTTGTACGAATTCTCCAGGACTCTGCTGCCCAAACACTTCAAGCACAACAATTTCTCAAGCTTCATCCGCCAGCTCAACACTTATGGGTTCAGAAAGGTTGATCCGGACAGGTGGGAGTTTGCAAATGAAAGGTTTCAGGGAGGGAAGAGGCACTTGCTGAAGAACATCAAGAGAAGGCGCAGATACAACAAGAAGCCGAATATGGTCTCTGCCGATTCCCCCAAACCCGGGTTGGAAGCCGAAATTGAGTCCCTAAAGAAAGACCAGGACTTTTTGAGATTGGAAATCTTGAATCTCAGACAACAGCAAAAGTACTCACAGCATCAACTAACTGCGTTTCAACAACGAATTCATAGTTCTTTGTGTAAGCAGCAAAGGATGCTCTTTTTCCTCACCAAAACCGCTGTGAATCCCATCTCTGTGCAGCAGCTAATGCAGAAGAGAATGATAAAGAGAGAGCTGGACGGTGGTGATCTAGGCAAGAGAAGGAGATTGCCTCCGGCCCAAGGCATTGAAAGCTTTGATGAGTGGATAAATGACAGCCTCAGTTTTGATTGTAGAAACCAAGTTCAGGAAGAACCGGTGCCTATGCAAACTGCACTTGCTGAACAAATCTCAGAGGCAATCGCTTCCCAACAAAATGGAACCCCGTTATCATCTCCCATGGTTGATAAATCATGTAATGCAGGTCAAGATCTAAATCCTCGTGTCATGGCCGGAACAAGCAGCTCGGAGGATATGCCCTCTGCTTATGATGACATGTCCGATAAATTTCTGGAAGAAAATATAGTTTCCAATGATGAATGTGCACTGAACGACTCCAGTTTTTACCAAGAATTGGAGGATTTGATTGCCGAGCCGTGCGATTGGAGTGCATATGCGAGTAACTCCTTGGTGGAGCAAGCTGGCTGCATTGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006979 GO:0006986 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010200 GO:0010243 GO:0010286 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033554 GO:0034605 GO:0034620 GO:0035966 GO:0035967 GO:0036293 GO:0036294 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071310 GO:0071453 GO:0071456 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HSF_DNA-bind

Protein Analysis

485

Amino Acids

54.03

Weight (kDa)

4.92

Isoelectric Point (pI)

63.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSF_DNA-bind	PF00447	148 - 237	2.6e-29	HSF-type DNA-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010515)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g07800
malus_domestica	MD02G1082000.v1.1 MD12G1184500.v1.1 MD15G1209400.v1.1
prunus_persica	Prupe.7G206900_v2.0.a1
pyrus_communis	pycom02g06490 pycom15g18570
rosa_chinensis	RchiOBHm_Chr2g0093671
rosa_laevigata	RLG00000016427
rosa_roxburghii	Rroxscaffold_2G00147910
rosa_rugosa	Rorug02G0038700 Rorug05G0356600
rosa_samantha	Rh2AG085100 Rh2BG085600 Rh2CG087900 Rh2DG083400 Rh3AG094700 Rh3CG099000 Rh3DG098400
rosa_wichuraiana	Rw0G013900 Rw3G008150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1211
AccB1I	GGYRCC	1 cut(s)	1117
AccB7I	CCANNNNNTGG	1 cut(s)	1372
AccBSI	CCGCTC	1 cut(s)	236
AccII	CGCG	1 cut(s)	423
AccIII	TCCGGA	1 cut(s)	637
AclWI	GGATC	3 cut(s)	2, 15, 629
AcoI	YGGCCR	2 cut(s)	252, 1248
AcsI	RAATTY	3 cut(s)	542, 882, 1304
AcuI	CTGAAG	1 cut(s)	713
AfaI	GTAC	2 cut(s)	539, 848
AfiI	CCNNNNNNNGG	7 cut(s)	17, 413, 761, 762, 1040, 1105, 1372
AflIII	ACRYGT	1 cut(s)	1293
AgeI	ACCGGT	2 cut(s)	207, 1114
AgsI	TTSAA	5 cut(s)	448, 574, 826, 875, 1049
AjnI	CCWGG	2 cut(s)	548, 798
Alw21I	GWGCWC	1 cut(s)	1345
Alw26I	GTCTC	2 cut(s)	33, 745
Alw44I	GTGCAC	1 cut(s)	1341
AlwI	GGATC	3 cut(s)	2, 15, 629
Ama87I	CYCGRG	1 cut(s)	761
Aor13HI	TCCGGA	1 cut(s)	637
AoxI	GGCC	5 cut(s)	53, 252, 430, 1035, 1248
ApaI	GGGCCC	1 cut(s)	434
ApaLI	GTGCAC	1 cut(s)	1341
ApeKI	GCWGC	7 cut(s)	397, 559, 904, 958, 961, 1260, 1443
ApoI	RAATTY	3 cut(s)	542, 882, 1304
AsiGI	ACCGGT	2 cut(s)	207, 1114
Asp700I	GAANNNNTTC	1 cut(s)	666
AspLEI	GCGC	2 cut(s)	423, 714
AspS9I	GGNCC	3 cut(s)	430, 431, 1036
AsuC2I	CCSGG	3 cut(s)	476, 762, 763
AsuHPI	GGTGA	5 cut(s)	190, 280, 371, 919, 1016
AvaI	CYCGRG	1 cut(s)	761
BaeGI	GKGCMC	2 cut(s)	434, 1345
BamHI	GGATCC	1 cut(s)	7
BanI	GGYRCC	1 cut(s)	1117
BanII	GRGCYC	1 cut(s)	434
BauI	CACGAG	1 cut(s)	1239
BbsI	GAAGAC	1 cut(s)	375
Bbv12I	GWGCWC	1 cut(s)	1345
BbvI	GCAGC	7 cut(s)	384, 546, 916, 970, 973, 1272, 1430
BccI	CCATC	3 cut(s)	344, 457, 956
BceAI	ACGGC	3 cut(s)	58, 358, 1378
BcgI	CGANNNNNNTGC	2 cut(s)	1388, 1422
BciT130I	CCWGG	2 cut(s)	550, 800
BcnI	CCSGG	3 cut(s)	476, 762, 763
BcoDI	GTCTC	2 cut(s)	33, 745
BfaI	CTAG	2 cut(s)	107, 1010
BfmI	CTRYAG	1 cut(s)	282
BfuAI	ACCTGC	1 cut(s)	1211
BglII	AGATCT	1 cut(s)	1228
BisI	GCNGC	9 cut(s)	252, 346, 398, 560, 905, 959, 962, 1261, 1444
BlpI	GCTNAGC	1 cut(s)	510
BlsI	GCNGC	9 cut(s)	253, 347, 399, 561, 906, 960, 963, 1262, 1445
BmcAI	AGTACT	1 cut(s)	848
Bme1390I	CCNGG	5 cut(s)	476, 550, 762, 763, 800
BmeT110I	CYCGRG	1 cut(s)	761
BmgT120I	GGNCC	3 cut(s)	430, 431, 1036
BmiI	GGNNCC	4 cut(s)	9, 432, 1119, 1180
BmrFI	CCNGG	5 cut(s)	476, 550, 762, 763, 800
BmsI	GCATC	3 cut(s)	496, 865, 903
BpiI	GAAGAC	1 cut(s)	375
BplI	GAGNNNNNCTC	4 cut(s)	493, 525, 1015, 1047
BpmI	CTGGAG	2 cut(s)	532, 1339
Bpu1102I	GCTNAGC	1 cut(s)	510
BpuEI	CTTGAG	1 cut(s)	574
BpuMI	CCSGG	3 cut(s)	476, 762, 763
BsaBI	GATNNNNATC	1 cut(s)	1233
BsaI	GGTCTC	1 cut(s)	745
BsaJI	CCNNGG	6 cut(s)	390, 408, 761, 1039, 1197, 1425
BsaWI	WCCGGW	3 cut(s)	207, 637, 1114
BsaXI	ACNNNNNCTCC	2 cut(s)	530, 560
Bsc4I	CCNNNNNNNGG	7 cut(s)	17, 413, 761, 762, 1040, 1105, 1372
Bse118I	RCCGGY	2 cut(s)	207, 1114
Bse1I	ACTGG	1 cut(s)	1356
Bse3DI	GCAATG	1 cut(s)	325
Bse8I	GATNNNNATC	1 cut(s)	1233
BseAI	TCCGGA	1 cut(s)	637
BseBI	CCWGG	2 cut(s)	550, 800
BseDI	CCNNGG	6 cut(s)	390, 408, 761, 1039, 1197, 1425
BseGI	GGATG	2 cut(s)	597, 918
BseJI	GATNNNNATC	1 cut(s)	1233
BseLI	CCNNNNNNNGG	7 cut(s)	17, 413, 761, 762, 1040, 1105, 1372
BseMI	GCAATG	1 cut(s)	325
BseMII	CTCAG	5 cut(s)	108, 524, 844, 1093, 1164
BseNI	ACTGG	1 cut(s)	1356
BseSI	GKGCMC	2 cut(s)	434, 1345
BseX3I	CGGCCG	1 cut(s)	252
BseXI	GCAGC	7 cut(s)	384, 546, 916, 970, 973, 1272, 1430
BseYI	CCCAGC	2 cut(s)	95, 247
BsgI	GTGCAG	2 cut(s)	977, 1116
Bsh1236I	CGCG	1 cut(s)	423
Bsh1285I	CGRYCG	2 cut(s)	255, 492
BshFI	GGCC	5 cut(s)	55, 254, 432, 1037, 1250
BshNI	GGYRCC	1 cut(s)	1117
BshTI	ACCGGT	2 cut(s)	207, 1114
BsiEI	CGRYCG	2 cut(s)	255, 492
BsiHKAI	GWGCWC	1 cut(s)	1345
BsiHKCI	CYCGRG	1 cut(s)	761
BsiSI	CCGG	7 cut(s)	208, 476, 638, 762, 1034, 1115, 1251
BslFI	GGGAC	2 cut(s)	545, 769
BslI	CCNNNNNNNGG	7 cut(s)	17, 413, 761, 762, 1040, 1105, 1372
BsmAI	GTCTC	2 cut(s)	33, 745
BsmBI	CGTCTC	1 cut(s)	33
BsmFI	GGGAC	2 cut(s)	545, 769
BsnI	GGCC	5 cut(s)	55, 254, 432, 1037, 1250
Bso31I	GGTCTC	1 cut(s)	745
BsoBI	CYCGRG	1 cut(s)	761
Bsp120I	GGGCCC	1 cut(s)	430
Bsp1286I	GDGCHC	2 cut(s)	434, 1345
Bsp13I	TCCGGA	1 cut(s)	637
Bsp143I	GATC	7 cut(s)	7, 130, 322, 489, 634, 1006, 1228
Bsp1720I	GCTNAGC	1 cut(s)	510
Bsp19I	CCATGG	3 cut(s)	390, 408, 1197
BspANI	GGCC	5 cut(s)	55, 254, 432, 1037, 1250
BspCNI	CTCAG	5 cut(s)	109, 523, 843, 1092, 1163
BspEI	TCCGGA	1 cut(s)	637
BspFNI	CGCG	1 cut(s)	423
BspLI	GGNNCC	4 cut(s)	9, 432, 1119, 1180
BspMI	ACCTGC	1 cut(s)	1211
BspPI	GGATC	3 cut(s)	2, 15, 629
BspT107I	GGYRCC	1 cut(s)	1117
BspTNI	GGTCTC	1 cut(s)	745
BsrBI	CCGCTC	1 cut(s)	236
BsrDI	GCAATG	1 cut(s)	325
BsrFI	RCCGGY	2 cut(s)	207, 1114
BsrI	ACTGG	1 cut(s)	1356
BssAI	RCCGGY	2 cut(s)	207, 1114
BssECI	CCNNGG	6 cut(s)	390, 408, 761, 1039, 1197, 1425
BssMI	GATC	7 cut(s)	7, 130, 322, 489, 634, 1006, 1228
BssSI	CACGAG	1 cut(s)	1239
BssT1I	CCWWGG	5 cut(s)	390, 408, 1039, 1197, 1425
Bst2BI	CACGAG	1 cut(s)	1239
Bst2UI	CCWGG	2 cut(s)	550, 800
Bst4CI	ACNGT	4 cut(s)	84, 283, 441, 1001
Bst6I	CTCTTC	4 cut(s)	223, 229, 674, 968
BstAPI	GCANNNNNTGC	2 cut(s)	913, 967
BstC8I	GCNNGC	4 cut(s)	430, 605, 1437, 1441
BstDEI	CTNAG	6 cut(s)	117, 310, 510, 830, 1079, 1150
BstDSI	CCRYGG	3 cut(s)	390, 408, 1197
BstF5I	GGATG	2 cut(s)	597, 918
BstFNI	CGCG	1 cut(s)	423
BstHHI	GCGC	2 cut(s)	423, 714
BstKTI	GATC	7 cut(s)	10, 133, 325, 492, 637, 1009, 1231
BstMAI	GTCTC	2 cut(s)	33, 745
BstMBI	GATC	7 cut(s)	7, 130, 322, 489, 634, 1006, 1228
BstMCI	CGRYCG	2 cut(s)	255, 492
BstMWI	GCNNNNNNNGC	9 cut(s)	30, 61, 351, 429, 600, 913, 967, 1050, 1449
BstNI	CCWGG	2 cut(s)	550, 800
BstNSI	RCATGY	1 cut(s)	1297
BstSCI	CCNGG	5 cut(s)	474, 548, 760, 761, 798
BstSFI	CTRYAG	1 cut(s)	282
BstSLI	GKGCMC	2 cut(s)	434, 1345
BstUI	CGCG	1 cut(s)	423
BstV1I	GCAGC	7 cut(s)	384, 546, 916, 970, 973, 1272, 1430
BstV2I	GAAGAC	1 cut(s)	375
BstX2I	RGATCY	2 cut(s)	7, 1228
BstXI	CCANNNNNNTGG	1 cut(s)	1175
BstYI	RGATCY	2 cut(s)	7, 1228
BstZI	CGGCCG	1 cut(s)	252
BsuRI	GGCC	5 cut(s)	55, 254, 432, 1037, 1250
BtgI	CCRYGG	3 cut(s)	390, 408, 1197
BtsCI	GGATG	2 cut(s)	597, 918
BtsIMutI	CAGTG	1 cut(s)	1343
BveI	ACCTGC	1 cut(s)	1211
Cac8I	GCNNGC	4 cut(s)	430, 605, 1437, 1441
CfoI	GCGC	2 cut(s)	423, 714
Cfr10I	RCCGGY	2 cut(s)	207, 1114
Cfr13I	GGNCC	3 cut(s)	430, 431, 1036
Cfr9I	CCCGGG	1 cut(s)	761
Csp6I	GTAC	2 cut(s)	538, 847
CspAI	ACCGGT	2 cut(s)	207, 1114
CspCI	CAANNNNNGTGG	2 cut(s)	426, 461
CviAII	CATG	6 cut(s)	391, 409, 1198, 1212, 1246, 1294
CviQI	GTAC	2 cut(s)	538, 847
DdeI	CTNAG	6 cut(s)	117, 310, 510, 830, 1079, 1150
DpnI	GATC	7 cut(s)	9, 132, 324, 491, 636, 1008, 1230
DpnII	GATC	7 cut(s)	7, 130, 322, 489, 634, 1006, 1228
EaeI	YGGCCR	2 cut(s)	252, 1248
EagI	CGGCCG	1 cut(s)	252
Eam1104I	CTCTTC	4 cut(s)	223, 229, 674, 968
EarI	CTCTTC	4 cut(s)	223, 229, 674, 968
EciI	GGCGGA	2 cut(s)	228, 590
EclXI	CGGCCG	1 cut(s)	252
Eco130I	CCWWGG	5 cut(s)	390, 408, 1039, 1197, 1425
Eco147I	AGGCCT	1 cut(s)	55
Eco24I	GRGCYC	1 cut(s)	434
Eco31I	GGTCTC	1 cut(s)	745
Eco52I	CGGCCG	1 cut(s)	252
Eco57I	CTGAAG	1 cut(s)	713
Eco88I	CYCGRG	1 cut(s)	761
EcoRI	GAATTC	2 cut(s)	542, 882
EcoRII	CCWGG	2 cut(s)	548, 798
EcoT14I	CCWWGG	5 cut(s)	390, 408, 1039, 1197, 1425
EcoT38I	GRGCYC	1 cut(s)	434
ErhI	CCWWGG	5 cut(s)	390, 408, 1039, 1197, 1425
Esp3I	CGTCTC	1 cut(s)	33
FaeI	CATG	6 cut(s)	394, 412, 1201, 1215, 1249, 1297
FalI	AAGNNNNNCTT	2 cut(s)	671, 703
FaqI	GGGAC	2 cut(s)	545, 769
FatI	CATG	6 cut(s)	390, 408, 1197, 1211, 1245, 1293
FauI	CCCGC	2 cut(s)	26, 421
FauNDI	CATATG	1 cut(s)	1411
Fnu4HI	GCNGC	9 cut(s)	252, 346, 398, 560, 905, 959, 962, 1261, 1444
FokI	GGATG	2 cut(s)	584, 925
FriOI	GRGCYC	1 cut(s)	434
Fsp4HI	GCNGC	9 cut(s)	252, 346, 398, 560, 905, 959, 962, 1261, 1444
FspBI	CTAG	2 cut(s)	107, 1010
GlaI	GCGC	2 cut(s)	422, 713
GluI	GCNGC	9 cut(s)	252, 346, 398, 560, 905, 959, 962, 1261, 1444
GsaI	CCCAGC	2 cut(s)	99, 251
GsuI	CTGGAG	2 cut(s)	532, 1339
HaeIII	GGCC	5 cut(s)	55, 254, 432, 1037, 1250
HapII	CCGG	7 cut(s)	208, 476, 638, 762, 1034, 1115, 1251
HhaI	GCGC	2 cut(s)	423, 714
Hin1II	CATG	6 cut(s)	394, 412, 1201, 1215, 1249, 1297
Hin6I	GCGC	2 cut(s)	421, 712
HinP1I	GCGC	2 cut(s)	421, 712
HincII	GTYRAC	1 cut(s)	87
HindII	GTYRAC	1 cut(s)	87
HindIII	AAGCTT	2 cut(s)	592, 1051
HinfI	GANTC	7 cut(s)	485, 553, 749, 782, 826, 943, 1352
HpaII	CCGG	7 cut(s)	208, 476, 638, 762, 1034, 1115, 1251
HphI	GGTGA	5 cut(s)	190, 280, 371, 919, 1016
Hpy166II	GTNNAC	3 cut(s)	87, 469, 1343
Hpy188I	TCNGA	7 cut(s)	190, 462, 626, 833, 1153, 1267, 1300
Hpy188III	TCNNGA	9 cut(s)	11, 107, 445, 638, 703, 823, 1106, 1226, 1310
Hpy8I	GTNNAC	3 cut(s)	87, 469, 1343
HpyAV	CCTTC	4 cut(s)	66, 185, 702, 1014
HpyCH4III	ACNGT	4 cut(s)	84, 283, 441, 1001
HpyCH4IV	ACGT	2 cut(s)	15, 455
HpyF10VI	GCNNNNNNNGC	9 cut(s)	30, 61, 351, 429, 600, 913, 967, 1050, 1449
HpyF3I	CTNAG	6 cut(s)	117, 310, 510, 830, 1079, 1150
HpySE526I	ACGT	2 cut(s)	15, 455
Hsp92II	CATG	6 cut(s)	394, 412, 1201, 1215, 1249, 1297
HspAI	GCGC	2 cut(s)	421, 712
Kpn2I	TCCGGA	1 cut(s)	637
Kzo9I	GATC	7 cut(s)	7, 130, 322, 489, 634, 1006, 1228
LmnI	GCTCC	3 cut(s)	241, 394, 1432
Lsp1109I	GCAGC	7 cut(s)	384, 546, 916, 970, 973, 1272, 1430
LweI	GCATC	3 cut(s)	496, 865, 903
MaeI	CTAG	2 cut(s)	107, 1010
MaeII	ACGT	2 cut(s)	15, 455
MaeIII	GTNAC	3 cut(s)	148, 178, 1418
MalI	GATC	7 cut(s)	9, 132, 324, 491, 636, 1008, 1230
MbiI	CCGCTC	1 cut(s)	236
MboI	GATC	7 cut(s)	7, 130, 322, 489, 634, 1006, 1228
MflI	RGATCY	2 cut(s)	7, 1228
MhlI	GDGCHC	2 cut(s)	434, 1345
MluCI	AATT	7 cut(s)	257, 542, 583, 777, 882, 1304, 1370
MlyI	GAGTC	3 cut(s)	547, 791, 1346
MmeI	TCCRAC	1 cut(s)	747
MroI	TCCGGA	1 cut(s)	637
MroXI	GAANNNNTTC	1 cut(s)	666
MslI	CAYNNNNRTG	1 cut(s)	953
MspA1I	CMGCKG	2 cut(s)	251, 938
MspI	CCGG	7 cut(s)	208, 476, 638, 762, 1034, 1115, 1251
MspR9I	CCNGG	5 cut(s)	476, 550, 762, 763, 800
MvaI	CCWGG	2 cut(s)	550, 800
MvnI	CGCG	1 cut(s)	423
MwoI	GCNNNNNNNGC	9 cut(s)	30, 61, 351, 429, 600, 913, 967, 1050, 1449
NciI	CCSGG	3 cut(s)	476, 762, 763
NcoI	CCATGG	3 cut(s)	390, 408, 1197
NdeI	CATATG	1 cut(s)	1411
NdeII	GATC	7 cut(s)	7, 130, 322, 489, 634, 1006, 1228
NlaIII	CATG	6 cut(s)	394, 412, 1201, 1215, 1249, 1297
NlaIV	GGNNCC	4 cut(s)	9, 432, 1119, 1180
NmeAIII	GCCGAG	1 cut(s)	1414
NmuCI	GTSAC	2 cut(s)	148, 178
NspI	RCATGY	1 cut(s)	1297
PceI	AGGCCT	1 cut(s)	55
PciI	ACATGT	1 cut(s)	1293
PcsI	WCGNNNNNNNCGW	2 cut(s)	377, 494
PdmI	GAANNNNTTC	1 cut(s)	666
PfeI	GAWTC	4 cut(s)	485, 749, 826, 943
PflMI	CCANNNNNTGG	1 cut(s)	1372
PfoI	TCCNGGA	1 cut(s)	548
PinAI	ACCGGT	2 cut(s)	207, 1114
PkrI	GCNGC	9 cut(s)	253, 347, 399, 561, 906, 960, 963, 1262, 1445
Ple19I	CGATCG	1 cut(s)	492
PleI	GAGTC	3 cut(s)	547, 790, 1346
PpsI	GAGTC	3 cut(s)	547, 790, 1346
PscI	ACATGT	1 cut(s)	1293
Psp6I	CCWGG	2 cut(s)	548, 798
PspFI	CCCAGC	2 cut(s)	95, 247
PspGI	CCWGG	2 cut(s)	548, 798
PspN4I	GGNNCC	4 cut(s)	9, 432, 1119, 1180
PspOMI	GGGCCC	1 cut(s)	430
PspPI	GGNCC	3 cut(s)	430, 431, 1036
PsuI	RGATCY	2 cut(s)	7, 1228
PvuI	CGATCG	1 cut(s)	492
RsaI	GTAC	2 cut(s)	539, 848
RsaNI	GTAC	2 cut(s)	538, 847
RseI	CAYNNNNRTG	1 cut(s)	953
SatI	GCNGC	9 cut(s)	252, 346, 398, 560, 905, 959, 962, 1261, 1444
Sau3AI	GATC	7 cut(s)	7, 130, 322, 489, 634, 1006, 1228
Sau96I	GGNCC	3 cut(s)	430, 431, 1036
ScaI	AGTACT	1 cut(s)	848
SchI	GAGTC	3 cut(s)	547, 791, 1346
ScrFI	CCNGG	5 cut(s)	476, 550, 762, 763, 800
SduI	GDGCHC	2 cut(s)	434, 1345
SfaNI	GCATC	3 cut(s)	496, 865, 903
SfcI	CTRYAG	1 cut(s)	282
SmaI	CCCGGG	1 cut(s)	763
SmiMI	CAYNNNNRTG	1 cut(s)	953
SmlI	CTYRAG	1 cut(s)	589
SmoI	CTYRAG	1 cut(s)	589
Sse9I	AATT	7 cut(s)	257, 542, 583, 777, 882, 1304, 1370
SseBI	AGGCCT	1 cut(s)	55
SspMI	CTAG	2 cut(s)	107, 1010
StuI	AGGCCT	1 cut(s)	55
StyD4I	CCNGG	5 cut(s)	474, 548, 760, 761, 798
StyI	CCWWGG	5 cut(s)	390, 408, 1039, 1197, 1425
TaaI	ACNGT	4 cut(s)	84, 283, 441, 1001
TaiI	ACGT	2 cut(s)	18, 458
TaqI	TCGA	2 cut(s)	133, 488
TasI	AATT	7 cut(s)	257, 542, 583, 777, 882, 1304, 1370
TatI	WGTACW	1 cut(s)	846
TauI	GCSGC	2 cut(s)	254, 348
TfiI	GAWTC	4 cut(s)	485, 749, 826, 943
TscAI	CASTG	1 cut(s)	1350
TseFI	GTSAC	2 cut(s)	148, 178
TseI	GCWGC	7 cut(s)	397, 559, 904, 958, 961, 1260, 1443
Tsp45I	GTSAC	2 cut(s)	148, 178
TspDTI	ATGAA	5 cut(s)	511, 586, 675, 875, 1350
TspGWI	ACGGA	1 cut(s)	60
TspMI	CCCGGG	1 cut(s)	761
TspRI	CASTG	1 cut(s)	1350
Van91I	CCANNNNNTGG	1 cut(s)	1372
VneI	GTGCAC	1 cut(s)	1341
XapI	RAATTY	3 cut(s)	542, 882, 1304
XbaI	TCTAGA	1 cut(s)	106
XceI	RCATGY	1 cut(s)	1297
XcmI	CCANNNNNNNNNTGG	1 cut(s)	763
XmaI	CCCGGG	1 cut(s)	761
XmnI	GAANNNNTTC	1 cut(s)	666
XspI	CTAG	2 cut(s)	107, 1010
ZrmI	AGTACT	1 cut(s)	848

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.