RLG00000016427

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010515
Physical Location & Seq
Forward (+)
6578445 .. 6580271
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016427

Sequence Viewer

Length: 1401 bp
ATGGTGGTTTCTGATGGCGGTTATGGTGGTGGATCTTGTTCGCCATTTTCATCGGCGGCCCCGATTACACAACCCAAAGAGGCTTCAGAAGAAGCTGTGAAAGTCAAGGAAGAAGAAGAGGCAGTGAATTGTAAAGGTGGTAGTTTTGATGGGTCATCTGCTTCTTCATCTTCCTCCACCGCTTCACCAAAGCTCACAGAGGAAGTGGAGGAACCAAACAAGGCCGTGGATATGGCTAAACCAGAGGTGGTCCAAATTAAAGAAGAGAGCATAGAGATTGTGGTGGATGATAATGATGGTAGAGATTGCTTCAATTGTGGTAGTGGTAGTAATATTAATGGATCATATTCTTCTTCTTCTTCAAAGGTGTTGCCCAAGCCCATGGAGTGTTTGCTCGAAACAGGGCCGCCACCTTTTCTCAACAAGACATTTCAGATGGTGGACGACCCGGAAACCGACCCGATTGTGTCGTGGAGCGAAGCTCGCCAAAGCTTCATTGTTTGGGACTGCCATGAGTTCTCCAAGACCATATTGCCTAAGTACTTCAAGCACAGCAATTTCTCAAGCTTCATTCGCCAGCTCAACACTTACGGATTCAAGAAGGTTGATCCGGATAGGTGGGAGTTTGCAAATGAAGGGTTTCAAGGAGGGAAGAAGCACTTGCTGAAAAGCATCAAGAGAAGAAGCAGATACAAGAAGCAGCCACAAGGAAGCAATGTCTCATCTAATTCAACCAAAAAAGCCAGGTTAGAAGTCCAACTCGAAACGCTGAAGAAGGATCAAGACGTACTGAAACTCGAAATCTTGAATCTCAAACAGCAACAGAAGTACTCGCAGCATCAGCTGACTGCTGTCGAAGAGAGGATTCGAAGTTCTGAGTGTAAGCAGCAGAGGATGCTGCTTTTCCTCACCAAGACAACAAGGAAACCCAATTTTGTGGAGCAGCTAATGCAGAAAAGAGTATTGAAGAGAGAGCTTGAGAGGGGCGATTTAGGAAGGAGGAGGAGGTTACTTTCAGCCCAAGGTTTTGAAGATTTTGGTGATTGTGGAGACCAAGTTCAAGTCTTTGCTGGGCAATTGCCGGAAGCAGTGGTTCCTCAACAGCAAGAAACCCCATTGCCAGCTTCCGTGGTTGATAAATCATGTAGTTCGCTTCAAAATAATAAGCCACAGGCAGAAGTGCACTCTGTTTACCATGATATGTCCGAGAAATTGCTCCAAGAGAATTCAGTTTTTGATGATGAATATGCAGTCAATGACTCAAACTTTTACCAGGAATTGGAGGATTTAATTACCGGGCCACACGACTTTGATGATTTGATTGTCAAGCCACGTGATTGGAGTGGATATGTTAGTAACTGCTTGGTTGAACAAGCTGGTTGTGTTGGATCAGTGCCTTGA

Protein Analysis

467

Amino Acids

52.18

Weight (kDa)

5.33

Isoelectric Point (pI)

58.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 139 - 228 2.9e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1279
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 4 cut(s) 18, 56, 180, 407
AclWI GGATC 5 cut(s) 40, 349, 602, 786, 1396
AcsI RAATTY 1 cut(s) 1225
AcuI CTGAAG 2 cut(s) 69, 791
AcvI CACGTG 1 cut(s) 1334
AfaI GTAC 3 cut(s) 542, 789, 830
AfiI CCNNNNNNNGG 1 cut(s) 1279
AjnI CCWGG 2 cut(s) 743, 1272
AloI GAACNNNNNNTCC 4 cut(s) 856, 888, 1041, 1073
Alw21I GWGCWC 1 cut(s) 1185
Alw26I GTCTC 2 cut(s) 724, 1044
Alw44I GTGCAC 1 cut(s) 1181
AlwI GGATC 5 cut(s) 40, 349, 602, 786, 1396
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 4 cut(s) 57, 222, 404, 1298
ApaLI GTGCAC 1 cut(s) 1181
ApeKI GCWGC 5 cut(s) 700, 835, 886, 898, 943
ApoI RAATTY 1 cut(s) 1225
AseI ATTAAT 1 cut(s) 336
Asp700I GAANNNNTTC 1 cut(s) 639
AspS9I GGNCC 4 cut(s) 58, 250, 404, 1298
AsuC2I CCSGG 2 cut(s) 449, 1297
AsuHPI GGTGA 3 cut(s) 177, 901, 1052
AsuII TTCGAA 1 cut(s) 868
AvaII GGWCC 1 cut(s) 250
BaeGI GKGCMC 1 cut(s) 1185
BbrPI CACGTG 1 cut(s) 1334
Bbv12I GWGCWC 1 cut(s) 1185
BbvI GCAGC 5 cut(s) 712, 847, 885, 898, 955
BccI CCATC 4 cut(s) 8, 143, 290, 430
BceAI ACGGC 1 cut(s) 209
BciT130I CCWGG 2 cut(s) 745, 1274
BcnI CCSGG 2 cut(s) 449, 1297
BcoDI GTCTC 2 cut(s) 724, 1044
BisI GCNGC 7 cut(s) 57, 407, 701, 836, 887, 899, 944
BlsI GCNGC 7 cut(s) 58, 408, 702, 837, 888, 900, 945
BmcAI AGTACT 2 cut(s) 542, 830
Bme1390I CCNGG 4 cut(s) 449, 745, 1274, 1297
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 4 cut(s) 58, 250, 404, 1298
BmiI GGNNCC 3 cut(s) 60, 213, 1095
BmrFI CCNGG 4 cut(s) 449, 745, 1274, 1297
BmsI GCATC 3 cut(s) 681, 847, 885
BoxI GACNNNNGTC 1 cut(s) 851
BplI GAGNNNNNCTC 2 cut(s) 466, 498
Bpu14I TTCGAA 1 cut(s) 868
BpuEI CTTGAG 2 cut(s) 547, 998
BpuMI CCSGG 2 cut(s) 449, 1297
BsaAI YACGTR 1 cut(s) 1334
BsaI GGTCTC 1 cut(s) 1044
BsaJI CCNNGG 4 cut(s) 225, 381, 1021, 1128
BsaWI WCCGGW 1 cut(s) 610
BsaXI ACNNNNNCTCC 2 cut(s) 1041, 1071
Bsc4I CCNNNNNNNGG 1 cut(s) 1279
Bse3DI GCAATG 2 cut(s) 721, 1115
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 2 cut(s) 745, 1274
BseDI CCNNGG 4 cut(s) 225, 381, 1021, 1128
BseGI GGATG 2 cut(s) 292, 900
BseLI CCNNNNNNNGG 1 cut(s) 1279
BseMI GCAATG 2 cut(s) 721, 1115
BseMII CTCAG 1 cut(s) 867
BseRI GAGGAG 2 cut(s) 1015, 1018
BseSI GKGCMC 1 cut(s) 1185
BseXI GCAGC 5 cut(s) 712, 847, 885, 898, 955
BseYI CCCAGC 1 cut(s) 1070
BshFI GGCC 4 cut(s) 59, 224, 406, 1300
BsiHKAI GWGCWC 1 cut(s) 1185
BsiSI CCGG 4 cut(s) 449, 611, 1082, 1296
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 1 cut(s) 1279
BsmAI GTCTC 2 cut(s) 724, 1044
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 4 cut(s) 59, 224, 406, 1300
Bso31I GGTCTC 1 cut(s) 1044
Bsp119I TTCGAA 1 cut(s) 868
Bsp1286I GDGCHC 1 cut(s) 1185
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 5 cut(s) 32, 341, 607, 778, 1388
Bsp19I CCATGG 1 cut(s) 381
BspACI CCGC 4 cut(s) 18, 56, 180, 407
BspANI GGCC 4 cut(s) 59, 224, 406, 1300
BspCNI CTCAG 1 cut(s) 868
BspEI TCCGGA 1 cut(s) 610
BspLI GGNNCC 3 cut(s) 60, 213, 1095
BspPI GGATC 5 cut(s) 40, 349, 602, 786, 1396
BspT104I TTCGAA 1 cut(s) 868
BspTNI GGTCTC 1 cut(s) 1044
BsrDI GCAATG 2 cut(s) 721, 1115
BssECI CCNNGG 4 cut(s) 225, 381, 1021, 1128
BssMI GATC 5 cut(s) 32, 341, 607, 778, 1388
BssT1I CCWWGG 2 cut(s) 381, 1021
Bst2UI CCWGG 2 cut(s) 745, 1274
Bst6I CTCTTC 4 cut(s) 111, 258, 852, 962
BstAPI GCANNNNNTGC 2 cut(s) 895, 949
BstBAI YACGTR 1 cut(s) 1334
BstBI TTCGAA 1 cut(s) 868
BstC8I GCNNGC 3 cut(s) 484, 578, 1122
BstDEI CTNAG 2 cut(s) 537, 876
BstDSI CCRYGG 3 cut(s) 225, 381, 1128
BstF5I GGATG 2 cut(s) 292, 900
BstKTI GATC 5 cut(s) 35, 344, 610, 781, 1391
BstMAI GTCTC 2 cut(s) 724, 1044
BstMBI GATC 5 cut(s) 32, 341, 607, 778, 1388
BstMWI GCNNNNNNNGC 5 cut(s) 483, 573, 841, 895, 949
BstNI CCWGG 2 cut(s) 745, 1274
BstPAI GACNNNNGTC 1 cut(s) 851
BstSCI CCNGG 4 cut(s) 447, 743, 1272, 1295
BstSLI GKGCMC 1 cut(s) 1185
BstV1I GCAGC 5 cut(s) 712, 847, 885, 898, 955
BstX2I RGATCY 1 cut(s) 32
BstXI CCANNNNNNTGG 3 cut(s) 382, 937, 1338
BstYI RGATCY 1 cut(s) 32
BsuRI GGCC 4 cut(s) 59, 224, 406, 1300
BtgI CCRYGG 3 cut(s) 225, 381, 1128
BtsCI GGATG 2 cut(s) 292, 900
BtsI GCAGTG 2 cut(s) 129, 1095
BtsIMutI CAGTG 3 cut(s) 129, 1095, 1398
Cac8I GCNNGC 3 cut(s) 484, 578, 1122
Cfr13I GGNCC 4 cut(s) 58, 250, 404, 1298
Csp6I GTAC 3 cut(s) 541, 788, 829
CviAII CATG 4 cut(s) 382, 512, 1143, 1196
CviQI GTAC 3 cut(s) 541, 788, 829
DdeI CTNAG 2 cut(s) 537, 876
DpnI GATC 5 cut(s) 34, 343, 609, 780, 1390
DpnII GATC 5 cut(s) 32, 341, 607, 778, 1388
Eam1104I CTCTTC 4 cut(s) 111, 258, 852, 962
EarI CTCTTC 4 cut(s) 111, 258, 852, 962
Eco130I CCWWGG 2 cut(s) 381, 1021
Eco31I GGTCTC 1 cut(s) 1044
Eco47I GGWCC 1 cut(s) 250
Eco57I CTGAAG 2 cut(s) 69, 791
Eco72I CACGTG 1 cut(s) 1334
EcoRI GAATTC 1 cut(s) 1225
EcoRII CCWGG 2 cut(s) 743, 1272
EcoT14I CCWWGG 2 cut(s) 381, 1021
ErhI CCWWGG 2 cut(s) 381, 1021
FaeI CATG 4 cut(s) 385, 515, 1146, 1199
FalI AAGNNNNNCTT 2 cut(s) 644, 676
FaqI GGGAC 1 cut(s) 518
FatI CATG 4 cut(s) 381, 511, 1142, 1195
Fnu4HI GCNGC 7 cut(s) 57, 407, 701, 836, 887, 899, 944
FokI GGATG 2 cut(s) 299, 907
Fsp4HI GCNGC 7 cut(s) 57, 407, 701, 836, 887, 899, 944
GluI GCNGC 7 cut(s) 57, 407, 701, 836, 887, 899, 944
GsaI CCCAGC 1 cut(s) 1074
HaeIII GGCC 4 cut(s) 59, 224, 406, 1300
HapII CCGG 4 cut(s) 449, 611, 1082, 1296
Hin1II CATG 4 cut(s) 385, 515, 1146, 1199
HindIII AAGCTT 2 cut(s) 490, 565
HinfI GANTC 4 cut(s) 594, 808, 865, 1259
HpaII CCGG 4 cut(s) 449, 611, 1082, 1296
HphI GGTGA 3 cut(s) 177, 901, 1052
Hpy166II GTNNAC 3 cut(s) 442, 1183, 1192
Hpy188I TCNGA 5 cut(s) 13, 88, 435, 877, 1207
Hpy188III TCNNGA 5 cut(s) 598, 611, 676, 782, 805
Hpy8I GTNNAC 3 cut(s) 442, 1183, 1192
HpyAV CCTTC 4 cut(s) 595, 629, 769, 990
HpyCH4IV ACGT 2 cut(s) 786, 1333
HpyCH4V TGCA 4 cut(s) 629, 952, 1183, 1250
HpyF10VI GCNNNNNNNGC 5 cut(s) 483, 573, 841, 895, 949
HpyF3I CTNAG 2 cut(s) 537, 876
HpySE526I ACGT 2 cut(s) 786, 1333
Hsp92II CATG 4 cut(s) 385, 515, 1146, 1199
Kpn2I TCCGGA 1 cut(s) 610
Kzo9I GATC 5 cut(s) 32, 341, 607, 778, 1388
LmnI GCTCC 3 cut(s) 474, 940, 1221
Lsp1109I GCAGC 5 cut(s) 712, 847, 885, 898, 955
LweI GCATC 3 cut(s) 681, 847, 885
MaeII ACGT 2 cut(s) 786, 1333
MaeIII GTNAC 2 cut(s) 1008, 1355
MalI GATC 5 cut(s) 34, 343, 609, 780, 1390
MboI GATC 5 cut(s) 32, 341, 607, 778, 1388
MfeI CAATTG 2 cut(s) 313, 1076
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 1 cut(s) 1185
MlyI GAGTC 1 cut(s) 1253
MmeI TCCRAC 2 cut(s) 781, 1366
MroI TCCGGA 1 cut(s) 610
MroXI GAANNNNTTC 1 cut(s) 639
MseI TTAA 3 cut(s) 258, 336, 1289
MspA1I CMGCKG 1 cut(s) 844
MspI CCGG 4 cut(s) 449, 611, 1082, 1296
MspR9I CCNGG 4 cut(s) 449, 745, 1274, 1297
MunI CAATTG 2 cut(s) 313, 1076
MvaI CCWGG 2 cut(s) 745, 1274
MwoI GCNNNNNNNGC 5 cut(s) 483, 573, 841, 895, 949
NciI CCSGG 2 cut(s) 449, 1297
NcoI CCATGG 1 cut(s) 381
NdeII GATC 5 cut(s) 32, 341, 607, 778, 1388
NlaIII CATG 4 cut(s) 385, 515, 1146, 1199
NlaIV GGNNCC 3 cut(s) 60, 213, 1095
NspV TTCGAA 1 cut(s) 868
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PdmI GAANNNNTTC 1 cut(s) 639
PfeI GAWTC 3 cut(s) 594, 808, 865
PflMI CCANNNNNTGG 1 cut(s) 1279
PkrI GCNGC 7 cut(s) 58, 408, 702, 837, 888, 900, 945
PleI GAGTC 1 cut(s) 1253
PmaCI CACGTG 1 cut(s) 1334
PmlI CACGTG 1 cut(s) 1334
PpsI GAGTC 1 cut(s) 1253
Ppu21I YACGTR 1 cut(s) 1334
PshAI GACNNNNGTC 1 cut(s) 851
PshBI ATTAAT 1 cut(s) 336
Psp6I CCWGG 2 cut(s) 743, 1272
PspCI CACGTG 1 cut(s) 1334
PspFI CCCAGC 1 cut(s) 1070
PspGI CCWGG 2 cut(s) 743, 1272
PspN4I GGNNCC 3 cut(s) 60, 213, 1095
PspPI GGNCC 4 cut(s) 58, 250, 404, 1298
PsuI RGATCY 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 844
RsaI GTAC 3 cut(s) 542, 789, 830
RsaNI GTAC 3 cut(s) 541, 788, 829
SaqAI TTAA 3 cut(s) 258, 336, 1289
SatI GCNGC 7 cut(s) 57, 407, 701, 836, 887, 899, 944
Sau3AI GATC 5 cut(s) 32, 341, 607, 778, 1388
Sau96I GGNCC 4 cut(s) 58, 250, 404, 1298
ScaI AGTACT 2 cut(s) 542, 830
SchI GAGTC 1 cut(s) 1253
ScrFI CCNGG 4 cut(s) 449, 745, 1274, 1297
SduI GDGCHC 1 cut(s) 1185
SfaNI GCATC 3 cut(s) 681, 847, 885
SfuI TTCGAA 1 cut(s) 868
SinI GGWCC 1 cut(s) 250
SmlI CTYRAG 2 cut(s) 562, 977
SmoI CTYRAG 2 cut(s) 562, 977
SsiI CCGC 4 cut(s) 18, 56, 180, 407
SspI AATATT 1 cut(s) 334
StyD4I CCNGG 4 cut(s) 447, 743, 1272, 1295
StyI CCWWGG 2 cut(s) 381, 1021
TaiI ACGT 2 cut(s) 789, 1336
TaqI TCGA 5 cut(s) 396, 762, 798, 855, 868
TatI WGTACW 2 cut(s) 540, 828
TauI GCSGC 2 cut(s) 59, 409
TfiI GAWTC 3 cut(s) 594, 808, 865
Tru1I TTAA 3 cut(s) 258, 336, 1289
Tru9I TTAA 3 cut(s) 258, 336, 1289
TscAI CASTG 3 cut(s) 129, 1095, 1398
TseI GCWGC 5 cut(s) 700, 835, 886, 898, 943
TspDTI ATGAA 6 cut(s) 39, 156, 484, 559, 648, 1257
TspGWI ACGGA 2 cut(s) 606, 1117
TspRI CASTG 3 cut(s) 129, 1095, 1398
Van91I CCANNNNNTGG 1 cut(s) 1279
VneI GTGCAC 1 cut(s) 1181
VpaK11BI GGWCC 1 cut(s) 250
VspI ATTAAT 1 cut(s) 336
XapI RAATTY 1 cut(s) 1225
XmnI GAANNNNTTC 1 cut(s) 639
ZrmI AGTACT 2 cut(s) 542, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.