Rh2BG085600

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010515
Physical Location & Seq
Forward (+)
6675868 .. 6678849
2982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG085600.1

Sequence Viewer

Length: 1404 bp
ATGGTGGTTTCTTACGGTGGTTATGGTGGCGGTGGATCTTGTTCGCCATTTTCATCGACGGCCCAGATTACACAACCCAAAGAGGCTTCAGAAGAAGCTGTGAAAGTCAAGGAAGAAGAAGAGGCAGTGAATTGTAAAGGTGGTAGTTTTGATGGGTCATCTGCTTCTTCATCTTCCTCCACCGCTTCACCAAAGCTCACAGAGGAAGTGGAGGAACCAAACAAGGCCGTGGAATTAGCGAAACCAGAGGTGGTCCAAATCAAAGCAGAGAGCATAGAGTTTGTGGTGGATGATTATGATGGTAGAGATTGCTTCAATTGTGGTAGTGGTAGTAATATTAATGGATCATATTCTTCTTCTTCTTCAAAGGTGTTGCCCAAGCCCATGGAGTGTTTGCTCGAAACAGGGCCGCCACCTTTTCTCAACAAGACATTTCAGATGGTGGACGACCCGGAAACCGACCTGATTGTGTCGTGGAGCGAAGCTCGCCAAAGCTTCATTGTTTGGGACTGCCATGAGTTCTCCAAGACCCTATTGCCTATGTACTTCAAGCACAGCAATTTCTCAAGCTTCATTCGCCAGCTCAACACTTATGGATTCAAGAAGGTTGATCCGGATAGGTGGGAGTTTGCAAATGAAGGGTTTCAAGGAGGGAAGAAGCACTTGCTGAAAAGCATCAAGAGAAGAAGCAGATACAACAAGCAGCCACAAGGAAGCAATGTCTCAGCTAATTCAACCAAAAAAGCCGGGTTAGAAGTCCAATTCGAAACGCTGAAGAAGGATCAAGACGTACTGAAACTCGAAATCTTGAATCTCAAACAGCAACAGAAGTACTCGCAGCATCAGCTGACTGCTGTCGAAGAGAGGATTCGAAGTTCTGAGTGTAAGCAGCAGAGGATGCTGCTTTTCCTCACCAAGACAACAAGGAAACCCAATTTTGTGGAGCAGCTAATGCAGAAAAGAGCGTTGAAGAGAGAGCTCGAGGGGGGCAATTTAGGAAAGAGAAGGAGGTTACTTTCAGCCCAAGGTTTTGAAGATTTTGGTGATTGTGGAGACCAAGTTCAAGTCTTGGCTGGGCAATTGCCGGAAGTAGTGGTTCCTCAACAGCAAGAAACCCCATTGCCAGCTTCCGTGGTTGATAAATCATGTAGTTCGCTTCAAAATAATAAGCCACAGGCAGAAGTGCACTTTGTTTACCATGATATGTCCGAGAAATTGCTCCAAGAGAATTCAGTTTTTGATGATGAATATGCAGTCAATGACTCAAACTTTTACCAGGAATTGGAGGATTTAATTACCGGGCCACACGACTTTGATGATTTGGTTGTCAAGCCACGTGATTGGAGTGGATATGTTAGTAACTGCTTGGTTGAACAAGCTGGTTGTGTTGGATCAATGCCTTGA

Protein Analysis

467

Amino Acids

52.21

Weight (kDa)

5.25

Isoelectric Point (pI)

57.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 140 - 229 6.9e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1282
AccIII TCCGGA 1 cut(s) 613
AciI CCGC 3 cut(s) 30, 183, 410
AclWI GGATC 5 cut(s) 43, 352, 605, 789, 1399
AcsI RAATTY 1 cut(s) 1228
AcuI CTGAAG 2 cut(s) 72, 794
AcvI CACGTG 1 cut(s) 1337
AfaI GTAC 3 cut(s) 545, 792, 833
AfiI CCNNNNNNNGG 1 cut(s) 1282
AjnI CCWGG 1 cut(s) 1275
AloI GAACNNNNNNTCC 4 cut(s) 859, 891, 1044, 1076
Alw21I GWGCWC 2 cut(s) 981, 1188
Alw26I GTCTC 2 cut(s) 727, 1047
Alw44I GTGCAC 1 cut(s) 1184
AlwI GGATC 5 cut(s) 43, 352, 605, 789, 1399
Ama87I CYCGRG 1 cut(s) 980
Aor13HI TCCGGA 1 cut(s) 613
AoxI GGCC 4 cut(s) 60, 225, 407, 1301
ApaLI GTGCAC 1 cut(s) 1184
ApeKI GCWGC 5 cut(s) 703, 838, 889, 901, 946
ApoI RAATTY 1 cut(s) 1228
AseI ATTAAT 1 cut(s) 339
Asp700I GAANNNNTTC 1 cut(s) 642
AspS9I GGNCC 4 cut(s) 61, 253, 407, 1301
AsuC2I CCSGG 3 cut(s) 452, 748, 1300
AsuHPI GGTGA 3 cut(s) 180, 904, 1055
AsuII TTCGAA 2 cut(s) 765, 871
AvaI CYCGRG 1 cut(s) 980
AvaII GGWCC 1 cut(s) 253
BaeGI GKGCMC 1 cut(s) 1188
BanII GRGCYC 1 cut(s) 981
BbrPI CACGTG 1 cut(s) 1337
Bbv12I GWGCWC 2 cut(s) 981, 1188
BbvI GCAGC 5 cut(s) 715, 850, 888, 901, 958
BccI CCATC 3 cut(s) 146, 293, 433
BceAI ACGGC 2 cut(s) 75, 212
BciT130I CCWGG 1 cut(s) 1277
BcnI CCSGG 3 cut(s) 452, 748, 1300
BcoDI GTCTC 2 cut(s) 727, 1047
BisI GCNGC 6 cut(s) 410, 704, 839, 890, 902, 947
BlsI GCNGC 6 cut(s) 411, 705, 840, 891, 903, 948
BmcAI AGTACT 1 cut(s) 833
Bme1390I CCNGG 4 cut(s) 452, 748, 1277, 1300
Bme18I GGWCC 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 980
BmgT120I GGNCC 4 cut(s) 61, 253, 407, 1301
BmiI GGNNCC 2 cut(s) 216, 1098
BmrFI CCNGG 4 cut(s) 452, 748, 1277, 1300
BmsI GCATC 3 cut(s) 684, 850, 888
BoxI GACNNNNGTC 1 cut(s) 854
BplI GAGNNNNNCTC 2 cut(s) 469, 501
Bpu14I TTCGAA 2 cut(s) 765, 871
BpuEI CTTGAG 1 cut(s) 550
BpuMI CCSGG 3 cut(s) 452, 748, 1300
BsaAI YACGTR 1 cut(s) 1337
BsaI GGTCTC 1 cut(s) 1047
BsaJI CCNNGG 4 cut(s) 228, 384, 1024, 1131
BsaWI WCCGGW 1 cut(s) 613
BsaXI ACNNNNNCTCC 2 cut(s) 1044, 1074
Bsc4I CCNNNNNNNGG 1 cut(s) 1282
Bse3DI GCAATG 2 cut(s) 724, 1118
BseAI TCCGGA 1 cut(s) 613
BseBI CCWGG 1 cut(s) 1277
BseDI CCNNGG 4 cut(s) 228, 384, 1024, 1131
BseGI GGATG 2 cut(s) 295, 903
BseLI CCNNNNNNNGG 1 cut(s) 1282
BseMI GCAATG 2 cut(s) 724, 1118
BseMII CTCAG 2 cut(s) 738, 870
BseSI GKGCMC 1 cut(s) 1188
BseXI GCAGC 5 cut(s) 715, 850, 888, 901, 958
BseYI CCCAGC 1 cut(s) 1073
BshFI GGCC 4 cut(s) 62, 227, 409, 1303
BsiHKAI GWGCWC 2 cut(s) 981, 1188
BsiHKCI CYCGRG 1 cut(s) 980
BsiSI CCGG 5 cut(s) 452, 614, 747, 1085, 1299
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 1 cut(s) 1282
BsmAI GTCTC 2 cut(s) 727, 1047
BsmFI GGGAC 1 cut(s) 521
BsnI GGCC 4 cut(s) 62, 227, 409, 1303
Bso31I GGTCTC 1 cut(s) 1047
BsoBI CYCGRG 1 cut(s) 980
Bsp119I TTCGAA 2 cut(s) 765, 871
Bsp1286I GDGCHC 2 cut(s) 981, 1188
Bsp13I TCCGGA 1 cut(s) 613
Bsp143I GATC 5 cut(s) 35, 344, 610, 781, 1391
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 3 cut(s) 30, 183, 410
BspANI GGCC 4 cut(s) 62, 227, 409, 1303
BspCNI CTCAG 2 cut(s) 737, 871
BspEI TCCGGA 1 cut(s) 613
BspLI GGNNCC 2 cut(s) 216, 1098
BspPI GGATC 5 cut(s) 43, 352, 605, 789, 1399
BspT104I TTCGAA 2 cut(s) 765, 871
BspTNI GGTCTC 1 cut(s) 1047
BsrDI GCAATG 2 cut(s) 724, 1118
BssECI CCNNGG 4 cut(s) 228, 384, 1024, 1131
BssMI GATC 5 cut(s) 35, 344, 610, 781, 1391
BssT1I CCWWGG 2 cut(s) 384, 1024
Bst2UI CCWGG 1 cut(s) 1277
Bst4CI ACNGT 1 cut(s) 17
Bst6I CTCTTC 3 cut(s) 114, 855, 965
BstAPI GCANNNNNTGC 2 cut(s) 898, 952
BstBAI YACGTR 1 cut(s) 1337
BstBI TTCGAA 2 cut(s) 765, 871
BstC8I GCNNGC 3 cut(s) 487, 581, 1125
BstDEI CTNAG 2 cut(s) 724, 879
BstDSI CCRYGG 3 cut(s) 228, 384, 1131
BstF5I GGATG 2 cut(s) 295, 903
BstKTI GATC 5 cut(s) 38, 347, 613, 784, 1394
BstMAI GTCTC 2 cut(s) 727, 1047
BstMBI GATC 5 cut(s) 35, 344, 610, 781, 1391
BstMWI GCNNNNNNNGC 5 cut(s) 486, 576, 844, 898, 952
BstNI CCWGG 1 cut(s) 1277
BstPAI GACNNNNGTC 1 cut(s) 854
BstSCI CCNGG 4 cut(s) 450, 746, 1275, 1298
BstSLI GKGCMC 1 cut(s) 1188
BstV1I GCAGC 5 cut(s) 715, 850, 888, 901, 958
BstX2I RGATCY 1 cut(s) 35
BstXI CCANNNNNNTGG 3 cut(s) 385, 940, 1341
BstYI RGATCY 1 cut(s) 35
BsuRI GGCC 4 cut(s) 62, 227, 409, 1303
BtgI CCRYGG 3 cut(s) 228, 384, 1131
BtsCI GGATG 2 cut(s) 295, 903
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 3 cut(s) 487, 581, 1125
Cfr13I GGNCC 4 cut(s) 61, 253, 407, 1301
Csp6I GTAC 3 cut(s) 544, 791, 832
CviAII CATG 4 cut(s) 385, 515, 1146, 1199
CviQI GTAC 3 cut(s) 544, 791, 832
DdeI CTNAG 2 cut(s) 724, 879
DpnI GATC 5 cut(s) 37, 346, 612, 783, 1393
DpnII GATC 5 cut(s) 35, 344, 610, 781, 1391
Eam1104I CTCTTC 3 cut(s) 114, 855, 965
EarI CTCTTC 3 cut(s) 114, 855, 965
Ecl136II GAGCTC 1 cut(s) 979
Eco130I CCWWGG 2 cut(s) 384, 1024
Eco24I GRGCYC 1 cut(s) 981
Eco31I GGTCTC 1 cut(s) 1047
Eco47I GGWCC 1 cut(s) 253
Eco53kI GAGCTC 1 cut(s) 979
Eco57I CTGAAG 2 cut(s) 72, 794
Eco72I CACGTG 1 cut(s) 1337
Eco88I CYCGRG 1 cut(s) 980
EcoICRI GAGCTC 1 cut(s) 979
EcoRI GAATTC 1 cut(s) 1228
EcoRII CCWGG 1 cut(s) 1275
EcoT14I CCWWGG 2 cut(s) 384, 1024
EcoT38I GRGCYC 1 cut(s) 981
ErhI CCWWGG 2 cut(s) 384, 1024
FaeI CATG 4 cut(s) 388, 518, 1149, 1202
FalI AAGNNNNNCTT 2 cut(s) 647, 679
FaqI GGGAC 1 cut(s) 521
FatI CATG 4 cut(s) 384, 514, 1145, 1198
Fnu4HI GCNGC 6 cut(s) 410, 704, 839, 890, 902, 947
FokI GGATG 2 cut(s) 302, 910
FriOI GRGCYC 1 cut(s) 981
Fsp4HI GCNGC 6 cut(s) 410, 704, 839, 890, 902, 947
GluI GCNGC 6 cut(s) 410, 704, 839, 890, 902, 947
GsaI CCCAGC 1 cut(s) 1077
HaeIII GGCC 4 cut(s) 62, 227, 409, 1303
HapII CCGG 5 cut(s) 452, 614, 747, 1085, 1299
Hin1II CATG 4 cut(s) 388, 518, 1149, 1202
HindIII AAGCTT 2 cut(s) 493, 568
HinfI GANTC 4 cut(s) 597, 811, 868, 1262
HpaII CCGG 5 cut(s) 452, 614, 747, 1085, 1299
HphI GGTGA 3 cut(s) 180, 904, 1055
Hpy166II GTNNAC 3 cut(s) 445, 1186, 1195
Hpy188I TCNGA 4 cut(s) 91, 438, 880, 1210
Hpy188III TCNNGA 5 cut(s) 601, 614, 679, 785, 808
Hpy8I GTNNAC 3 cut(s) 445, 1186, 1195
Hpy99I CGWCG 1 cut(s) 61
HpyAV CCTTC 4 cut(s) 598, 632, 772, 999
HpyCH4III ACNGT 1 cut(s) 17
HpyCH4IV ACGT 2 cut(s) 789, 1336
HpyCH4V TGCA 4 cut(s) 632, 955, 1186, 1253
HpyF10VI GCNNNNNNNGC 5 cut(s) 486, 576, 844, 898, 952
HpyF3I CTNAG 2 cut(s) 724, 879
HpySE526I ACGT 2 cut(s) 789, 1336
Hsp92II CATG 4 cut(s) 388, 518, 1149, 1202
Kpn2I TCCGGA 1 cut(s) 613
Kzo9I GATC 5 cut(s) 35, 344, 610, 781, 1391
LmnI GCTCC 3 cut(s) 477, 943, 1224
Lsp1109I GCAGC 5 cut(s) 715, 850, 888, 901, 958
LweI GCATC 3 cut(s) 684, 850, 888
MaeII ACGT 2 cut(s) 789, 1336
MaeIII GTNAC 2 cut(s) 1011, 1358
MalI GATC 5 cut(s) 37, 346, 612, 783, 1393
MboI GATC 5 cut(s) 35, 344, 610, 781, 1391
MfeI CAATTG 2 cut(s) 316, 1079
MflI RGATCY 1 cut(s) 35
MhlI GDGCHC 2 cut(s) 981, 1188
MlyI GAGTC 1 cut(s) 1256
MmeI TCCRAC 1 cut(s) 1369
MroI TCCGGA 1 cut(s) 613
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 2 cut(s) 339, 1292
MspA1I CMGCKG 1 cut(s) 847
MspI CCGG 5 cut(s) 452, 614, 747, 1085, 1299
MspR9I CCNGG 4 cut(s) 452, 748, 1277, 1300
MunI CAATTG 2 cut(s) 316, 1079
MvaI CCWGG 1 cut(s) 1277
MwoI GCNNNNNNNGC 5 cut(s) 486, 576, 844, 898, 952
NciI CCSGG 3 cut(s) 452, 748, 1300
NcoI CCATGG 1 cut(s) 384
NdeII GATC 5 cut(s) 35, 344, 610, 781, 1391
NlaIII CATG 4 cut(s) 388, 518, 1149, 1202
NlaIV GGNNCC 2 cut(s) 216, 1098
NspV TTCGAA 2 cut(s) 765, 871
PaeR7I CTCGAG 1 cut(s) 980
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 3 cut(s) 597, 811, 868
PflMI CCANNNNNTGG 1 cut(s) 1282
PkrI GCNGC 6 cut(s) 411, 705, 840, 891, 903, 948
PleI GAGTC 1 cut(s) 1256
PmaCI CACGTG 1 cut(s) 1337
PmlI CACGTG 1 cut(s) 1337
PpsI GAGTC 1 cut(s) 1256
Ppu21I YACGTR 1 cut(s) 1337
PshAI GACNNNNGTC 1 cut(s) 854
PshBI ATTAAT 1 cut(s) 339
Psp124BI GAGCTC 1 cut(s) 981
Psp6I CCWGG 1 cut(s) 1275
PspCI CACGTG 1 cut(s) 1337
PspFI CCCAGC 1 cut(s) 1073
PspGI CCWGG 1 cut(s) 1275
PspN4I GGNNCC 2 cut(s) 216, 1098
PspPI GGNCC 4 cut(s) 61, 253, 407, 1301
PspXI VCTCGAGB 1 cut(s) 980
PsuI RGATCY 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 847
RsaI GTAC 3 cut(s) 545, 792, 833
RsaNI GTAC 3 cut(s) 544, 791, 832
SacI GAGCTC 1 cut(s) 981
SaqAI TTAA 2 cut(s) 339, 1292
SatI GCNGC 6 cut(s) 410, 704, 839, 890, 902, 947
Sau3AI GATC 5 cut(s) 35, 344, 610, 781, 1391
Sau96I GGNCC 4 cut(s) 61, 253, 407, 1301
ScaI AGTACT 1 cut(s) 833
SchI GAGTC 1 cut(s) 1256
ScrFI CCNGG 4 cut(s) 452, 748, 1277, 1300
SduI GDGCHC 2 cut(s) 981, 1188
SfaNI GCATC 3 cut(s) 684, 850, 888
Sfr274I CTCGAG 1 cut(s) 980
SfuI TTCGAA 2 cut(s) 765, 871
SinI GGWCC 1 cut(s) 253
SlaI CTCGAG 1 cut(s) 980
SmlI CTYRAG 2 cut(s) 565, 980
SmoI CTYRAG 2 cut(s) 565, 980
SsiI CCGC 3 cut(s) 30, 183, 410
SspI AATATT 1 cut(s) 337
SstI GAGCTC 1 cut(s) 981
StyD4I CCNGG 4 cut(s) 450, 746, 1275, 1298
StyI CCWWGG 2 cut(s) 384, 1024
TaaI ACNGT 1 cut(s) 17
TaiI ACGT 2 cut(s) 792, 1339
TaqI TCGA 7 cut(s) 56, 399, 765, 801, 858, 871, 981
TatI WGTACW 2 cut(s) 543, 831
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 3 cut(s) 597, 811, 868
Tru1I TTAA 2 cut(s) 339, 1292
Tru9I TTAA 2 cut(s) 339, 1292
TscAI CASTG 1 cut(s) 132
TseI GCWGC 5 cut(s) 703, 838, 889, 901, 946
TspDTI ATGAA 6 cut(s) 42, 159, 487, 562, 651, 1260
TspGWI ACGGA 1 cut(s) 1120
TspRI CASTG 1 cut(s) 132
Van91I CCANNNNNTGG 1 cut(s) 1282
VneI GTGCAC 1 cut(s) 1184
VpaK11BI GGWCC 1 cut(s) 253
VspI ATTAAT 1 cut(s) 339
XapI RAATTY 1 cut(s) 1228
XhoI CTCGAG 1 cut(s) 980
XmnI GAANNNNTTC 1 cut(s) 642
ZrmI AGTACT 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.