Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010515

Physical Location & Seq

Genomic Coordinates Forward (+)

16799506 .. 16801891

Gene Structure 2386 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1209400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGGTGGTTCCCGACGGTGGTGAAAGTTCTGGCGGTGGAGACGGTGATGGGACGCCTTCCCGGCCATTGTCGTCGAAAACCCAGTTTCCAAAAACCAGCAATGGTTTGAAACAATCAGAGAACGAGCTTGATCAAGTCAAAGAAGAAGTGACCGAACTGAAAAAAGAGCTAAAGGCATTGACCTCTTCCAAAGGCGGAAACTTTGACCTCTCGTCTTCCTCGCCTTCCTCCGCCGCTCCGCCGAACCCAGCGGCGGAATTGCTCCACGTGAAGGAAGAAACAGTAGATGTTGTGGTTGTGGAGGATGATGACTTCGACGGTGGAGACCACGTCAACACCATCAACGGCTGTGGTGGTGATTTTGGGTCTTCTTCCATGGAGCTGCCTAAACCCATGGAGGGTTTGCGCAAGGTGGGCCCACCGCCGTTCTTGAACAAGACTTTTCAGATGGTGGACGACCCGGAAACCGATTCCGTCGTTTCGTGGAGTGATACTCGGCAGAGCTTCATTGTTTGGGACTTGTACGAATTCTCCAGGACTCTCCTGCCCAAATACTTCAAGCACAACAATATCTCAAGCTTTATTCGCCAGCTCAACACTTATGGATTCAAAAAGGTTGATCCGGACAGGTGGGAGTTTGCAAGTGAAGGGTTCCAGGGTGGGAAGAAGCACTTGCTGAAGAACATCAAGAGAAGAAGAAGGTATAACAAGAAGCCGACCCCAGTCGCTGCCCATTCAACCAAAGCCAGGTTGGAAGCCGAAATTGAGTCCCTAAAAAACGACCAGGACTTTTTGAGGTTGGAAATCTTGAATCTCAGACAACAGCAAAAGTACTCTCAGCATCAACTGACTGCGGTTGAACAACGAATTCGAAGTTCCGTGTGTAAGCAGCAAAGTATGCTCTTTTTCCTCACCAAAACCGCCATGAATCCCATTTTTGTGCAGCAGCTAATGCTGAAGAGAGTGATAAAGAGAGAGTTGGATGGAGGTGATCTAGGCAAGAGAAGGAGATTGTCCCCGGCCCAAGACATCGAAAGCTTTGATGAGTGGATAAATGCCAGCCTCAGTTTTGATTGTGGAGGCCAAATTGAGGAAGAACTGGTGCCTATGCAGTCTGCACTTGCTGAACAAATCTCAGAGGGAATTGCTTCCAAACAAAATGAAACCCCGTTATCATCTCCCAAAGTTGATAAATCATGTAATGCAGGTCAAGATCTAAATCCTTGTGTGATGGCCGGAACAAGCAGCTCGGAGGATGTGCCCTCTGCTTATGATGACATGTCCGATAAATTTCTGGAGGAGTATATAGTTTTCAATGATGAATGTGCACTGAACGACTCCAGTTTATACCAAGAATTGGAGGATTTGATTGTCAAGCCGTGCGATTGGAGCGCATATGTGAGTAACTCCTTGGTGGAGCAAGCTGGTTGCATTGGCTCATATAAATCTAAGAATGAAGATGCAACCACAGGTGAATCAAGAACTCCTCAATCTTCTTGGTTAAGAACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006979 GO:0006986 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010200 GO:0010243 GO:0010286 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033554 GO:0034605 GO:0034620 GO:0035966 GO:0035967 GO:0036293 GO:0036294 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071310 GO:0071453 GO:0071456 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HSF_DNA-bind

Protein Analysis

504

Amino Acids

55.99

Weight (kDa)

5.0

Isoelectric Point (pI)

61.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSF_DNA-bind	PF00447	143 - 232	1.1e-28	HSF-type DNA-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010515)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g07800
malus_domestica	MD02G1082000.v1.1 MD12G1184500.v1.1 MD15G1209400.v1.1
prunus_persica	Prupe.7G206900_v2.0.a1
pyrus_communis	pycom02g06490 pycom15g18570
rosa_chinensis	RchiOBHm_Chr2g0093671
rosa_laevigata	RLG00000016427
rosa_roxburghii	Rroxscaffold_2G00147910
rosa_rugosa	Rorug02G0038700 Rorug05G0356600
rosa_samantha	Rh2AG085100 Rh2BG085600 Rh2CG087900 Rh2DG083400 Rh3AG094700 Rh3CG099000 Rh3DG098400
rosa_wichuraiana	Rw0G013900 Rw3G008150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	407
Acc36I	ACCTGC	1 cut(s)	1196
AccB1I	GGYRCC	1 cut(s)	1102
AccB7I	CCANNNNNTGG	1 cut(s)	1357
AccBSI	CCGCTC	1 cut(s)	236
AccIII	TCCGGA	1 cut(s)	622
AclWI	GGATC	1 cut(s)	614
AcoI	YGGCCR	2 cut(s)	62, 1233
AcsI	RAATTY	3 cut(s)	527, 867, 1289
AcuI	CTGAAG	2 cut(s)	698, 977
AcvI	CACGTG	1 cut(s)	268
AcyI	GRCGYC	1 cut(s)	53
AfaI	GTAC	2 cut(s)	524, 833
AfiI	CCNNNNNNNGG	7 cut(s)	17, 253, 271, 398, 747, 1090, 1357
AflIII	ACRYGT	1 cut(s)	1278
AgsI	TTSAA	8 cut(s)	109, 433, 559, 610, 738, 811, 860, 1315
AhdI	GACNNNNNGTC	1 cut(s)	211
AjiI	CACGTC	1 cut(s)	331
AjnI	CCWGG	4 cut(s)	533, 654, 746, 783
Alw21I	GWGCWC	1 cut(s)	1330
Alw26I	GTCTC	2 cut(s)	33, 318
Alw44I	GTGCAC	1 cut(s)	1326
AlwI	GGATC	1 cut(s)	614
AlwNI	CAGNNNCTG	1 cut(s)	728
Aor13HI	TCCGGA	1 cut(s)	622
AoxI	GGCC	5 cut(s)	62, 415, 1020, 1081, 1233
ApaI	GGGCCC	1 cut(s)	419
ApaLI	GTGCAC	1 cut(s)	1326
ApeKI	GCWGC	6 cut(s)	382, 728, 889, 943, 946, 1245
ApoI	RAATTY	3 cut(s)	527, 867, 1289
Asp700I	GAANNNNTTC	1 cut(s)	1147
AspLEI	GCGC	2 cut(s)	408, 1394
AspS9I	GGNCC	3 cut(s)	415, 416, 1021
AsuC2I	CCSGG	3 cut(s)	61, 461, 1019
AsuHPI	GGTGA	6 cut(s)	32, 56, 368, 904, 1001, 1484
AsuII	TTCGAA	1 cut(s)	871
BaeGI	GKGCMC	3 cut(s)	419, 1263, 1330
BanI	GGYRCC	1 cut(s)	1102
BanII	GRGCYC	1 cut(s)	419
BbrPI	CACGTG	1 cut(s)	268
BbsI	GAAGAC	2 cut(s)	207, 360
Bbv12I	GWGCWC	1 cut(s)	1330
BbvI	GCAGC	6 cut(s)	369, 715, 901, 955, 958, 1257
BccI	CCATC	5 cut(s)	41, 347, 442, 977, 1225
BceAI	ACGGC	3 cut(s)	361, 409, 1363
BciT130I	CCWGG	4 cut(s)	535, 656, 748, 785
BclI	TGATCA	1 cut(s)	130
BcnI	CCSGG	3 cut(s)	61, 461, 1019
BcoDI	GTCTC	2 cut(s)	33, 318
BfaI	CTAG	1 cut(s)	995
BfuAI	ACCTGC	1 cut(s)	1196
BglI	GCCNNNNNGGC	1 cut(s)	61
BglII	AGATCT	1 cut(s)	1213
BisI	GCNGC	8 cut(s)	234, 252, 383, 729, 890, 944, 947, 1246
BlsI	GCNGC	8 cut(s)	235, 253, 384, 730, 891, 945, 948, 1247
BmcAI	AGTACT	1 cut(s)	833
Bme1390I	CCNGG	7 cut(s)	61, 461, 535, 656, 748, 785, 1019
BmeRI	GACNNNNNGTC	1 cut(s)	211
BmgBI	CACGTC	1 cut(s)	331
BmgT120I	GGNCC	3 cut(s)	415, 416, 1021
BmiI	GGNNCC	4 cut(s)	9, 417, 653, 1104
BmrFI	CCNGG	7 cut(s)	61, 461, 535, 656, 748, 785, 1019
BmrI	ACTGGG	2 cut(s)	76, 716
BmsI	GCATC	2 cut(s)	850, 1450
BmuI	ACTGGG	2 cut(s)	76, 716
BoxI	GACNNNNGTC	1 cut(s)	722
BpiI	GAAGAC	2 cut(s)	207, 360
BplI	GAGNNNNNCTC	2 cut(s)	478, 510
BpmI	CTGGAG	3 cut(s)	517, 1316, 1324
Bpu14I	TTCGAA	1 cut(s)	871
BpuEI	CTTGAG	1 cut(s)	559
BpuMI	CCSGG	3 cut(s)	61, 461, 1019
BsaAI	YACGTR	1 cut(s)	268
BsaBI	GATNNNNATC	1 cut(s)	1218
BsaHI	GRCGYC	1 cut(s)	53
BsaI	GGTCTC	1 cut(s)	318
BsaJI	CCNNGG	5 cut(s)	375, 393, 655, 1017, 1410
BsaWI	WCCGGW	1 cut(s)	622
BsaXI	ACNNNNNCTCC	4 cut(s)	315, 345, 515, 545
Bsc4I	CCNNNNNNNGG	7 cut(s)	17, 253, 271, 398, 747, 1090, 1357
Bse1I	ACTGG	4 cut(s)	82, 722, 1104, 1341
Bse3DI	GCAATG	1 cut(s)	106
Bse8I	GATNNNNATC	1 cut(s)	1218
BseAI	TCCGGA	1 cut(s)	622
BseBI	CCWGG	4 cut(s)	535, 656, 748, 785
BseDI	CCNNGG	5 cut(s)	375, 393, 655, 1017, 1410
BseGI	GGATG	3 cut(s)	310, 988, 1261
BseJI	GATNNNNATC	1 cut(s)	1218
BseLI	CCNNNNNNNGG	7 cut(s)	17, 253, 271, 398, 747, 1090, 1357
BseMI	GCAATG	1 cut(s)	106
BseMII	CTCAG	4 cut(s)	829, 851, 1078, 1149
BseNI	ACTGG	4 cut(s)	82, 722, 1104, 1341
BseRI	GAGGAG	2 cut(s)	1313, 1476
BseSI	GKGCMC	3 cut(s)	419, 1263, 1330
BseXI	GCAGC	6 cut(s)	369, 715, 901, 955, 958, 1257
BseYI	CCCAGC	1 cut(s)	247
BsgI	GTGCAG	2 cut(s)	962, 1101
BshFI	GGCC	5 cut(s)	64, 417, 1022, 1083, 1235
BshNI	GGYRCC	1 cut(s)	1102
BsiHKAI	GWGCWC	1 cut(s)	1330
BsiSI	CCGG	5 cut(s)	61, 461, 623, 1019, 1236
BslFI	GGGAC	4 cut(s)	64, 530, 754, 1000
BslI	CCNNNNNNNGG	7 cut(s)	17, 253, 271, 398, 747, 1090, 1357
BsmAI	GTCTC	2 cut(s)	33, 318
BsmBI	CGTCTC	1 cut(s)	33
BsmFI	GGGAC	4 cut(s)	64, 530, 754, 1000
BsnI	GGCC	5 cut(s)	64, 417, 1022, 1083, 1235
Bso31I	GGTCTC	1 cut(s)	318
Bsp119I	TTCGAA	1 cut(s)	871
Bsp120I	GGGCCC	1 cut(s)	415
Bsp1286I	GDGCHC	3 cut(s)	419, 1263, 1330
Bsp13I	TCCGGA	1 cut(s)	622
Bsp143I	GATC	4 cut(s)	130, 619, 991, 1213
Bsp19I	CCATGG	2 cut(s)	375, 393
BspANI	GGCC	5 cut(s)	64, 417, 1022, 1083, 1235
BspCNI	CTCAG	4 cut(s)	828, 850, 1077, 1148
BspEI	TCCGGA	1 cut(s)	622
BspLI	GGNNCC	4 cut(s)	9, 417, 653, 1104
BspMI	ACCTGC	1 cut(s)	1196
BspPI	GGATC	1 cut(s)	614
BspT104I	TTCGAA	1 cut(s)	871
BspT107I	GGYRCC	1 cut(s)	1102
BspTNI	GGTCTC	1 cut(s)	318
BsrBI	CCGCTC	1 cut(s)	236
BsrDI	GCAATG	1 cut(s)	106
BsrI	ACTGG	4 cut(s)	82, 722, 1104, 1341
BssECI	CCNNGG	5 cut(s)	375, 393, 655, 1017, 1410
BssMI	GATC	4 cut(s)	130, 619, 991, 1213
BssNI	GRCGYC	1 cut(s)	53
BssT1I	CCWWGG	3 cut(s)	375, 393, 1410
Bst2UI	CCWGG	4 cut(s)	535, 656, 748, 785
Bst4CI	ACNGT	4 cut(s)	17, 44, 283, 320
Bst6I	CTCTTC	2 cut(s)	190, 953
BstACI	GRCGYC	1 cut(s)	53
BstAPI	GCANNNNNTGC	2 cut(s)	898, 952
BstBAI	YACGTR	1 cut(s)	268
BstBI	TTCGAA	1 cut(s)	871
BstC8I	GCNNGC	3 cut(s)	590, 1060, 1422
BstDEI	CTNAG	5 cut(s)	815, 837, 1064, 1135, 1449
BstDSI	CCRYGG	2 cut(s)	375, 393
BstF5I	GGATG	3 cut(s)	310, 988, 1261
BstHHI	GCGC	2 cut(s)	408, 1394
BstKTI	GATC	4 cut(s)	133, 622, 994, 1216
BstMAI	GTCTC	2 cut(s)	33, 318
BstMBI	GATC	4 cut(s)	130, 619, 991, 1213
BstMWI	GCNNNNNNNGC	6 cut(s)	61, 414, 585, 898, 952, 1389
BstNI	CCWGG	4 cut(s)	535, 656, 748, 785
BstNSI	RCATGY	1 cut(s)	1282
BstPAI	GACNNNNGTC	1 cut(s)	722
BstSCI	CCNGG	7 cut(s)	59, 459, 533, 654, 746, 783, 1017
BstSLI	GKGCMC	3 cut(s)	419, 1263, 1330
BstV1I	GCAGC	6 cut(s)	369, 715, 901, 955, 958, 1257
BstV2I	GAAGAC	2 cut(s)	207, 360
BstX2I	RGATCY	1 cut(s)	1213
BstYI	RGATCY	1 cut(s)	1213
BsuRI	GGCC	5 cut(s)	64, 417, 1022, 1083, 1235
BtgI	CCRYGG	2 cut(s)	375, 393
BtrI	CACGTC	1 cut(s)	331
BtsCI	GGATG	3 cut(s)	310, 988, 1261
BtsIMutI	CAGTG	1 cut(s)	1328
BveI	ACCTGC	1 cut(s)	1196
Cac8I	GCNNGC	3 cut(s)	590, 1060, 1422
CaiI	CAGNNNCTG	1 cut(s)	728
CfoI	GCGC	2 cut(s)	408, 1394
Cfr13I	GGNCC	3 cut(s)	415, 416, 1021
CseI	GACGC	1 cut(s)	61
Csp6I	GTAC	2 cut(s)	523, 832
CspCI	CAANNNNNGTGG	2 cut(s)	331, 366
CviAII	CATG	5 cut(s)	376, 394, 925, 1197, 1279
CviQI	GTAC	2 cut(s)	523, 832
DdeI	CTNAG	5 cut(s)	815, 837, 1064, 1135, 1449
DpnI	GATC	4 cut(s)	132, 621, 993, 1215
DpnII	GATC	4 cut(s)	130, 619, 991, 1213
DriI	GACNNNNNGTC	1 cut(s)	211
EaeI	YGGCCR	2 cut(s)	62, 1233
Eam1104I	CTCTTC	2 cut(s)	190, 953
Eam1105I	GACNNNNNGTC	1 cut(s)	211
EarI	CTCTTC	2 cut(s)	190, 953
EciI	GGCGGA	4 cut(s)	210, 220, 228, 269
Eco130I	CCWWGG	3 cut(s)	375, 393, 1410
Eco24I	GRGCYC	1 cut(s)	419
Eco31I	GGTCTC	1 cut(s)	318
Eco57I	CTGAAG	2 cut(s)	698, 977
Eco72I	CACGTG	1 cut(s)	268
EcoRI	GAATTC	2 cut(s)	527, 867
EcoRII	CCWGG	4 cut(s)	533, 654, 746, 783
EcoT14I	CCWWGG	3 cut(s)	375, 393, 1410
EcoT38I	GRGCYC	1 cut(s)	419
ErhI	CCWWGG	3 cut(s)	375, 393, 1410
Esp3I	CGTCTC	1 cut(s)	33
FaeI	CATG	5 cut(s)	379, 397, 928, 1200, 1282
FalI	AAGNNNNNCTT	2 cut(s)	656, 688
FaqI	GGGAC	4 cut(s)	64, 530, 754, 1000
FatI	CATG	5 cut(s)	375, 393, 924, 1196, 1278
FauNDI	CATATG	1 cut(s)	1396
FbaI	TGATCA	1 cut(s)	130
Fnu4HI	GCNGC	8 cut(s)	234, 252, 383, 729, 890, 944, 947, 1246
FokI	GGATG	3 cut(s)	317, 995, 1268
FriOI	GRGCYC	1 cut(s)	419
Fsp4HI	GCNGC	8 cut(s)	234, 252, 383, 729, 890, 944, 947, 1246
FspBI	CTAG	1 cut(s)	995
FspI	TGCGCA	1 cut(s)	407
GlaI	GCGC	2 cut(s)	407, 1393
GluI	GCNGC	8 cut(s)	234, 252, 383, 729, 890, 944, 947, 1246
GsaI	CCCAGC	1 cut(s)	251
GsuI	CTGGAG	3 cut(s)	517, 1316, 1324
HaeIII	GGCC	5 cut(s)	64, 417, 1022, 1083, 1235
HapII	CCGG	5 cut(s)	61, 461, 623, 1019, 1236
HgaI	GACGC	1 cut(s)	61
HhaI	GCGC	2 cut(s)	408, 1394
Hin1I	GRCGYC	1 cut(s)	53
Hin1II	CATG	5 cut(s)	379, 397, 928, 1200, 1282
Hin6I	GCGC	2 cut(s)	406, 1392
HinP1I	GCGC	2 cut(s)	406, 1392
HincII	GTYRAC	1 cut(s)	334
HindII	GTYRAC	1 cut(s)	334
HindIII	AAGCTT	2 cut(s)	577, 1036
HinfI	GANTC	8 cut(s)	470, 538, 606, 767, 811, 928, 1337, 1475
HpaII	CCGG	5 cut(s)	61, 461, 623, 1019, 1236
HphI	GGTGA	6 cut(s)	32, 56, 368, 904, 1001, 1484
Hpy166II	GTNNAC	3 cut(s)	334, 454, 1328
Hpy188I	TCNGA	6 cut(s)	118, 447, 818, 1138, 1252, 1285
Hpy188III	TCNNGA	8 cut(s)	11, 430, 623, 688, 808, 1211, 1295, 1479
Hpy8I	GTNNAC	3 cut(s)	334, 454, 1328
Hpy99I	CGWCG	4 cut(s)	17, 76, 320, 479
HpyAV	CCTTC	6 cut(s)	66, 234, 265, 641, 693, 999
HpyCH4III	ACNGT	4 cut(s)	17, 44, 283, 320
HpyCH4IV	ACGT	3 cut(s)	267, 330, 1508
HpyCH4V	TGCA	8 cut(s)	641, 943, 1111, 1118, 1205, 1328, 1431, 1463
HpyF10VI	GCNNNNNNNGC	6 cut(s)	61, 414, 585, 898, 952, 1389
HpyF3I	CTNAG	5 cut(s)	815, 837, 1064, 1135, 1449
HpySE526I	ACGT	3 cut(s)	267, 330, 1508
Hsp92I	GRCGYC	1 cut(s)	53
Hsp92II	CATG	5 cut(s)	379, 397, 928, 1200, 1282
HspAI	GCGC	2 cut(s)	406, 1392
Kpn2I	TCCGGA	1 cut(s)	622
Ksp22I	TGATCA	1 cut(s)	130
Kzo9I	GATC	4 cut(s)	130, 619, 991, 1213
LmnI	GCTCC	5 cut(s)	241, 267, 379, 1389, 1417
Lsp1109I	GCAGC	6 cut(s)	369, 715, 901, 955, 958, 1257
LweI	GCATC	2 cut(s)	850, 1450
MaeI	CTAG	1 cut(s)	995
MaeII	ACGT	3 cut(s)	267, 330, 1508
MaeIII	GTNAC	2 cut(s)	148, 1403
MalI	GATC	4 cut(s)	132, 621, 993, 1215
MbiI	CCGCTC	1 cut(s)	236
MboI	GATC	4 cut(s)	130, 619, 991, 1213
MflI	RGATCY	1 cut(s)	1213
MhlI	GDGCHC	3 cut(s)	419, 1263, 1330
MluCI	AATT	8 cut(s)	257, 527, 762, 867, 1086, 1143, 1289, 1355
MlyI	GAGTC	3 cut(s)	532, 776, 1331
MmeI	TCCRAC	3 cut(s)	732, 780, 960
MroI	TCCGGA	1 cut(s)	622
MroXI	GAANNNNTTC	1 cut(s)	1147
MseI	TTAA	1 cut(s)	1502
MslI	CAYNNNNRTG	1 cut(s)	938
MspA1I	CMGCKG	1 cut(s)	251
MspI	CCGG	5 cut(s)	61, 461, 623, 1019, 1236
MspR9I	CCNGG	7 cut(s)	61, 461, 535, 656, 748, 785, 1019
MvaI	CCWGG	4 cut(s)	535, 656, 748, 785
MwoI	GCNNNNNNNGC	6 cut(s)	61, 414, 585, 898, 952, 1389
NciI	CCSGG	3 cut(s)	61, 461, 1019
NcoI	CCATGG	2 cut(s)	375, 393
NdeI	CATATG	1 cut(s)	1396
NdeII	GATC	4 cut(s)	130, 619, 991, 1213
NlaIII	CATG	5 cut(s)	379, 397, 928, 1200, 1282
NlaIV	GGNNCC	4 cut(s)	9, 417, 653, 1104
NmeAIII	GCCGAG	1 cut(s)	475
NmuCI	GTSAC	1 cut(s)	148
NsbI	TGCGCA	1 cut(s)	407
NspI	RCATGY	1 cut(s)	1282
NspV	TTCGAA	1 cut(s)	871
PciI	ACATGT	1 cut(s)	1278
PdmI	GAANNNNTTC	1 cut(s)	1147
PfeI	GAWTC	5 cut(s)	470, 606, 811, 928, 1475
PflFI	GACNNNGTC	1 cut(s)	329
PflMI	CCANNNNNTGG	1 cut(s)	1357
PfoI	TCCNGGA	1 cut(s)	533
PkrI	GCNGC	8 cut(s)	235, 253, 384, 730, 891, 945, 948, 1247
PleI	GAGTC	3 cut(s)	532, 775, 1331
PmaCI	CACGTG	1 cut(s)	268
PmlI	CACGTG	1 cut(s)	268
PpsI	GAGTC	3 cut(s)	532, 775, 1331
Ppu21I	YACGTR	1 cut(s)	268
PscI	ACATGT	1 cut(s)	1278
PshAI	GACNNNNGTC	1 cut(s)	722
Psp6I	CCWGG	4 cut(s)	533, 654, 746, 783
PspCI	CACGTG	1 cut(s)	268
PspFI	CCCAGC	1 cut(s)	247
PspGI	CCWGG	4 cut(s)	533, 654, 746, 783
PspN4I	GGNNCC	4 cut(s)	9, 417, 653, 1104
PspOMI	GGGCCC	1 cut(s)	415
PspPI	GGNCC	3 cut(s)	415, 416, 1021
PstNI	CAGNNNCTG	1 cut(s)	728
PsuI	RGATCY	1 cut(s)	1213
PsyI	GACNNNGTC	1 cut(s)	329
RsaI	GTAC	2 cut(s)	524, 833
RsaNI	GTAC	2 cut(s)	523, 832
RseI	CAYNNNNRTG	1 cut(s)	938
SaqAI	TTAA	1 cut(s)	1502
SatI	GCNGC	8 cut(s)	234, 252, 383, 729, 890, 944, 947, 1246
Sau3AI	GATC	4 cut(s)	130, 619, 991, 1213
Sau96I	GGNCC	3 cut(s)	415, 416, 1021
ScaI	AGTACT	1 cut(s)	833
SchI	GAGTC	3 cut(s)	532, 776, 1331
ScrFI	CCNGG	7 cut(s)	61, 461, 535, 656, 748, 785, 1019
SduI	GDGCHC	3 cut(s)	419, 1263, 1330
SfaNI	GCATC	2 cut(s)	850, 1450
SfuI	TTCGAA	1 cut(s)	871
SmiMI	CAYNNNNRTG	1 cut(s)	938
SmlI	CTYRAG	1 cut(s)	574
SmoI	CTYRAG	1 cut(s)	574
Sse9I	AATT	8 cut(s)	257, 527, 762, 867, 1086, 1143, 1289, 1355
SspMI	CTAG	1 cut(s)	995
StyD4I	CCNGG	7 cut(s)	59, 459, 533, 654, 746, 783, 1017
StyI	CCWWGG	3 cut(s)	375, 393, 1410
TaaI	ACNGT	4 cut(s)	17, 44, 283, 320
TaiI	ACGT	3 cut(s)	270, 333, 1511
TaqI	TCGA	4 cut(s)	74, 315, 871, 1032
TaqII	GACCGA	1 cut(s)	167
TasI	AATT	8 cut(s)	257, 527, 762, 867, 1086, 1143, 1289, 1355
TatI	WGTACW	1 cut(s)	831
TauI	GCSGC	2 cut(s)	236, 254
TfiI	GAWTC	5 cut(s)	470, 606, 811, 928, 1475
Tru1I	TTAA	1 cut(s)	1502
Tru9I	TTAA	1 cut(s)	1502
TscAI	CASTG	1 cut(s)	1335
TseFI	GTSAC	1 cut(s)	148
TseI	GCWGC	6 cut(s)	382, 728, 889, 943, 946, 1245
Tsp45I	GTSAC	1 cut(s)	148
TspDTI	ATGAA	5 cut(s)	496, 941, 1176, 1335, 1470
TspGWI	ACGGA	2 cut(s)	463, 868
TspRI	CASTG	1 cut(s)	1335
Tth111I	GACNNNGTC	1 cut(s)	329
Van91I	CCANNNNNTGG	1 cut(s)	1357
VneI	GTGCAC	1 cut(s)	1326
XapI	RAATTY	3 cut(s)	527, 867, 1289
XceI	RCATGY	1 cut(s)	1282
XcmI	CCANNNNNNNNNTGG	1 cut(s)	748
XmnI	GAANNNNTTC	1 cut(s)	1147
XspI	CTAG	1 cut(s)	995
ZrmI	AGTACT	1 cut(s)	833

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.