MD02G1093500.v1.1

subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013828
Physical Location & Seq
Forward (+)
7440989 .. 7443787
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1093500.v1.1.491

Sequence Viewer

Length: 2469 bp
ATGTCGCATTCGAGATCCCCGCATGCTTATAAGCCCGACATCGGTCTCTTTCTCCTTCTACCCTCCTCTATTCCTTTCAGGCAGTCGCGGCTCCAGAGCGACTGTACTCTCTCACTCTCTCTTCATCTTCCACATTTGGATATACTAAATCTACCCAGCTCAAGCTTTTTAGCCATGGCCTCCGGGGCCTTCTGGTTCGGAGCTCTGCTCCTTCTTCTTCCATGTCTCTCAGCCATGGCGAAGCAGACTTACATTGTGCACATGAACCAACACTCCAAGCCGGAATCCTACGCGACCCACCATGACTGGTACTCCGCCAGCCTCCAGTCCCTCTCCTCCGACTCAGATTCCCTCCTTTACACCTACACCGACGCCTACCACGGCTTCGCCGCCTCCTTAGATCCGGACCAGGCCGAGCTGCTCCGCCAGTCCGACTCTGTCATTGGCGTCTACGAAGACACGGTTTACAACCTCCACACCACTCGTACCCCCGAGTTCCTGGGTCTTGATACCGAATCGGGACTCTGGGCGGGTCACTCGACCCAGGATTTGAACCAGGCATCCAACGACGTTATCATTGGAGTTCTCGACACAGGGGTCTGGCCCGAGTCCAAGAGCTTCGACGATACCGGAATGCCCGAGATCCCGACCAAGTGGCGCGGCCAGTGCGAATCTGCCCCCGATTTTGCTGCTACTCTCTGCAACAAGAAGTTAATCGGAGCTCGGAGCTTCTCCAAAGGCTATCAAATGGCCTCCGGCGGAAGCAACTTGAGAAAGCCGAAGGAAGTCGTGTCCGCTCGCGACATAGACGGTCACGGCACCCACACCTCCAGCACCGCCGCCGGGTCCCTCGTGGCCAACGCCAGCCTCCTCGGATACGCGAGCGGAACGGCTCGCGGAATGGCTCCTCACGCGCGCGTCGCCGCCTACAAGGTCTGCTGGAGCACCGGATGTTTCGGTTCGGACATTATCGCGGGAATGGATCGCGCCATCATCGACGGCGTCGACGTCTTGTCGCTCTCTCTAGGCGGTGGCTCCGCTCCTTACTACCGCGACACGATCGCGATTGGCGCGTTCACGGCTATGGAGAGGGGTATTTTCGTCTCATGCTCGGCCGGGAACAGTGGACCCACTGAGGCCTCGTTGGCCAACACAGCCCCATGGATCATGACCGTTGGAGCCGGAACCTTGGATCGGGATTTCCCGGCTTACGCTTTGCTAGGAAACAAACTCCGGTTCACCGGCGTCTCTCTTTACAGCGGCACCGGAATGGGAAACAAACCGGTCCAGTTGGTTTACAATAAGGGGTCCAACGGCTCTAGTAACTTGTGCCTACCCGGTTCGCTCAACCCGGATTTGGTGCGTGGGAAAGTGGTGATGTGCGACCGAGGCGTCAACGCACGTGTCGAGAAAGGTGGCGTCGTGCGCGCAGCTGGTGGGATTGGGATGATAATCGCCAACACCGCCGCCAGCGGTGAGGAGTTGGTGGCTGACAGTCACTTGCTACCAGCTGTCGCCGTCGGAAGAAAAGTCGGTGATCAAATCAGGGCATACGCTCAGCACGATCCCAATCCTACGGCTGTTATTACCTTCGGCGGGACCGTGTTGAATGTACGGCCGTCACCGGTCGTGGCGGCGTTTAGTTCACGGGGGCCCAATACTGTGAACCCCCAGATCTTGAAGCCGGACGTGATTGGTCCAGGTGTCAACATTTTGGCAGCGTGGTCGGAGGCGATCGGGCCCACCGGGCTTGAGGAGGACAAAAGGAAGAGCCAGTTCAACATCATGTCAGGTACATCAATGTCATGCCCACACATTAGTGGGCTTGCCGCACTGCTCAAGGCGGCCCATCCAGAATGGAGCCCAAGCGCTGTGAAATCTGCCCTAATGACCACAGCTTACACCCATGACAACACCAACTCCCCTCTCCGAGACGCGGCCGACGGCACGCTTTCTAACCCGTGGGCGCACGGCTCCGGCCATGTCGACCCCTCGAAGGCGCTCTCCCCGGGCCTGGTTTACGACACCACCGCCGAGGACTACATTGCATTTTTGTGCTCATTGGAGTACACCAATGAGCATGTCCAAGCCATTGTCAAGAGGCCCAATGTTACTTGTGCGAGAAAGTATTCGGACCCCGGCCAGCTCAACTACCCTTCCTTCTCAATCGTGTTCGGGAGCAAAAACAAAAGGGTTGTGAGGTACACCCGGGAATTGACGAATGTCGGCGCAGCCGGCTCTGTATACAGAGCCTCCGTGACCAGTCCTTCGACGGTGAGGACCATCGTGAAGCCCACAAGGCTCGTGTTCAACAATGTAGGAGAGAAGCAGAAGTACACGGTCACATTTGTGGCTTTGCCGGGTGCAGATAAAACAGCAAGGTCGGAGTTTGGGTCAATTGTGTGGGCAAACCCACAACACCAAGTCAAAAGCCCGGTTACTTTTGCATGGACGCAGTTGATAGACTAA

Protein Analysis

823

Amino Acids

87.46

Weight (kDa)

7.32

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 83 - 159 2.1e-19 Peptidase inhibitor I9
Peptidase_S8 PF00082 189 - 644 8.4e-45 Subtilase family
PA PF02225 430 - 515 1.7e-12 PA domain
fn3_6 PF17766 715 - 814 4.9e-26 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05920
fragaria_vesca FvH4_1g08840
malus_domestica MD02G1093500.v1.1 MD15G1218800.v1.1
prunus_persica Prupe.7G197100_v2.0.a1
pyrus_communis pycom02g07370 pycom15g19400
rosa_chinensis RchiOBHm_Chr2g0095311
rosa_laevigata RLG00000016553
rosa_multiflora Rmu_ssc0000183.1_g000049
rosa_roxburghii Rroxscaffold_2G00146490
rosa_rugosa Rorug02G0049200
rosa_samantha Rh2AG097600 Rh2BG098800 Rh2CG100900 Rh2DG098500
rosa_wichuraiana Rw2G007360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 30
AasI GACNNNNNNGTC 2 cut(s) 596, 1391
AatII GACGTC 1 cut(s) 1011
AccB1I GGYRCC 2 cut(s) 818, 1262
AccBSI CCGCTC 3 cut(s) 797, 885, 1040
AccI GTMKAC 4 cut(s) 450, 1005, 1986, 2244
AccIII TCCGGA 1 cut(s) 403
AclWI GGATC 7 cut(s) 9, 395, 637, 990, 1172, 1200, 1559
AcoI YGGCCR 8 cut(s) 661, 855, 1113, 1146, 1616, 1938, 1978, 2140
AcvI CACGTG 1 cut(s) 1403
AcyI GRCGYC 7 cut(s) 372, 447, 1002, 1008, 1245, 1392, 1419
AfaI GTAC 8 cut(s) 106, 311, 487, 1614, 1795, 2069, 2204, 2336
AfeI AGCGCT 1 cut(s) 1870
AfiI CCNNNNNNNGG 8 cut(s) 41, 843, 1194, 1357, 1596, 1936, 1996, 2014
AflIII ACRYGT 1 cut(s) 1402
AgeI ACCGGT 2 cut(s) 1282, 1624
AgsI TTSAA 5 cut(s) 553, 1609, 1681, 1780, 2311
AhdI GACNNNNNGTC 1 cut(s) 1012
AjiI CACGTC 1 cut(s) 1690
AjnI CCWGG 6 cut(s) 408, 498, 543, 555, 1699, 2013
AleI CACNNNNGTG 2 cut(s) 1818, 2348
AloI GAACNNNNNNTCC 4 cut(s) 257, 289, 545, 577
Alw21I GWGCWC 5 cut(s) 205, 261, 724, 947, 2060
Alw26I GTCTC 5 cut(s) 50, 230, 1108, 1252, 1926
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 7 cut(s) 9, 395, 637, 990, 1172, 1200, 1559
Ama87I CYCGRG 5 cut(s) 491, 605, 638, 2008, 2208
Aor13HI TCCGGA 1 cut(s) 403
Aor51HI AGCGCT 1 cut(s) 1870
ApaI GGGCCC 2 cut(s) 1656, 1743
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 5 cut(s) 418, 689, 1430, 1718, 2231
ArsI GACNNNNNNTTYG 4 cut(s) 532, 564, 2251, 2283
AsiGI ACCGGT 2 cut(s) 1282, 1624
Asp700I GAANNNNTTC 1 cut(s) 2128
AsuHPI GGTGA 6 cut(s) 1231, 1387, 1487, 1547, 1614, 2287
AvaI CYCGRG 5 cut(s) 491, 605, 638, 2008, 2208
AvaII GGWCC 9 cut(s) 406, 846, 1127, 1285, 1308, 1599, 1697, 2134, 2280
BaeGI GKGCMC 3 cut(s) 261, 1656, 1743
BalI TGGCCA 2 cut(s) 857, 1148
BanI GGYRCC 2 cut(s) 818, 1262
BanII GRGCYC 5 cut(s) 205, 724, 1656, 1743, 1865
BarI GAAGNNNNNNTAC 4 cut(s) 233, 265, 2318, 2350
BauI CACGAG 2 cut(s) 851, 2303
BbrPI CACGTG 1 cut(s) 1403
BbsI GAAGAC 1 cut(s) 462
Bbv12I GWGCWC 5 cut(s) 205, 261, 724, 947, 2060
BbvI GCAGC 5 cut(s) 405, 676, 1442, 1730, 2243
BccI CCATC 3 cut(s) 998, 1857, 2291
BcgI CGANNNNNNTGC 4 cut(s) 671, 705, 1707, 1741
BciT130I CCWGG 6 cut(s) 410, 500, 545, 557, 1701, 2015
BciVI GTATCC 1 cut(s) 869
BclI TGATCA 1 cut(s) 1537
BcoDI GTCTC 5 cut(s) 50, 230, 1108, 1252, 1926
BfaI CTAG 3 cut(s) 1025, 1220, 1320
BfoI RGCGCY 2 cut(s) 1872, 2003
BfuI GTATCC 1 cut(s) 869
BglI GCCNNNNNGGC 4 cut(s) 185, 1145, 1747, 2299
BglII AGATCT 1 cut(s) 1674
BlpI GCTNAGC 1 cut(s) 1557
Bme18I GGWCC 9 cut(s) 406, 846, 1127, 1285, 1308, 1599, 1697, 2134, 2280
BmeRI GACNNNNNGTC 1 cut(s) 1012
BmeT110I CYCGRG 5 cut(s) 491, 605, 638, 2008, 2208
BmgBI CACGTC 1 cut(s) 1690
BmsI GCATC 1 cut(s) 569
BoxI GACNNNNGTC 2 cut(s) 42, 2222
BpiI GAAGAC 1 cut(s) 462
BplI GAGNNNNNCTC 2 cut(s) 192, 224
BpmI CTGGAG 4 cut(s) 77, 308, 814, 961
Bpu1102I GCTNAGC 1 cut(s) 1557
BpuEI CTTGAG 4 cut(s) 145, 790, 1772, 1823
BsaAI YACGTR 1 cut(s) 1403
BsaBI GATNNNNATC 2 cut(s) 1451, 1569
BsaHI GRCGYC 7 cut(s) 372, 447, 1002, 1008, 1245, 1392, 1419
BsaI GGTCTC 1 cut(s) 50
BsaWI WCCGGW 7 cut(s) 403, 629, 947, 1233, 1265, 1282, 1624
Bsc4I CCNNNNNNNGG 8 cut(s) 41, 843, 1194, 1357, 1596, 1936, 1996, 2014
Bse118I RCCGGY 4 cut(s) 1241, 1282, 1624, 2234
Bse1I ACTGG 7 cut(s) 311, 325, 427, 664, 1288, 1774, 2262
Bse3DI GCAATG 1 cut(s) 2043
Bse8I GATNNNNATC 2 cut(s) 1451, 1569
BseAI TCCGGA 1 cut(s) 403
BseBI CCWGG 6 cut(s) 410, 500, 545, 557, 1701, 2015
BseGI GGATG 4 cut(s) 560, 956, 1452, 1849
BseJI GATNNNNATC 2 cut(s) 1451, 1569
BseLI CCNNNNNNNGG 8 cut(s) 41, 843, 1194, 1357, 1596, 1936, 1996, 2014
BseMI GCAATG 1 cut(s) 2043
BseMII CTCAG 4 cut(s) 243, 357, 1125, 1571
BseNI ACTGG 7 cut(s) 311, 325, 427, 664, 1288, 1774, 2262
BsePI GCGCGC 2 cut(s) 914, 1426
BseRI GAGGAG 6 cut(s) 55, 325, 860, 897, 1493, 1769
BseSI GKGCMC 3 cut(s) 261, 1656, 1743
BseX3I CGGCCG 3 cut(s) 1113, 1616, 1938
BseXI GCAGC 5 cut(s) 405, 676, 1442, 1730, 2243
BseYI CCCAGC 1 cut(s) 155
BsgI GTGCAG 1 cut(s) 2385
Bsh1285I CGRYCG 7 cut(s) 1062, 1116, 1387, 1619, 1629, 1737, 1941
BshNI GGYRCC 2 cut(s) 818, 1262
BshTI ACCGGT 2 cut(s) 1282, 1624
BsiEI CGRYCG 7 cut(s) 1062, 1116, 1387, 1619, 1629, 1737, 1941
BsiHKAI GWGCWC 5 cut(s) 205, 261, 724, 947, 2060
BsiHKCI CYCGRG 5 cut(s) 491, 605, 638, 2008, 2208
BslFI GGGAC 4 cut(s) 313, 534, 832, 1612
BslI CCNNNNNNNGG 8 cut(s) 41, 843, 1194, 1357, 1596, 1936, 1996, 2014
BsmAI GTCTC 5 cut(s) 50, 230, 1108, 1252, 1926
BsmBI CGTCTC 3 cut(s) 1108, 1252, 1926
BsmFI GGGAC 4 cut(s) 313, 534, 832, 1612
BsmI GAATGC 2 cut(s) 7, 639
Bso31I GGTCTC 1 cut(s) 50
BsoBI CYCGRG 5 cut(s) 491, 605, 638, 2008, 2208
Bsp120I GGGCCC 2 cut(s) 1652, 1739
Bsp1286I GDGCHC 8 cut(s) 205, 261, 724, 947, 1656, 1743, 1865, 2060
Bsp13I TCCGGA 1 cut(s) 403
Bsp1720I GCTNAGC 1 cut(s) 1557
Bsp19I CCATGG 3 cut(s) 174, 234, 1160
Bsp68I TCGCGA 2 cut(s) 801, 1064
BspCNI CTCAG 4 cut(s) 242, 356, 1126, 1570
BspEI TCCGGA 1 cut(s) 403
BspHI TCATGA 1 cut(s) 1167
BspPI GGATC 7 cut(s) 9, 395, 637, 990, 1172, 1200, 1559
BspQI GCTCTTC 1 cut(s) 1763
BspT107I GGYRCC 2 cut(s) 818, 1262
BspTNI GGTCTC 1 cut(s) 50
BsrBI CCGCTC 3 cut(s) 797, 885, 1040
BsrDI GCAATG 1 cut(s) 2043
BsrFI RCCGGY 4 cut(s) 1241, 1282, 1624, 2234
BsrI ACTGG 7 cut(s) 311, 325, 427, 664, 1288, 1774, 2262
BssAI RCCGGY 4 cut(s) 1241, 1282, 1624, 2234
BssHII GCGCGC 2 cut(s) 914, 1426
BssNAI GTATAC 1 cut(s) 2245
BssNI GRCGYC 7 cut(s) 372, 447, 1002, 1008, 1245, 1392, 1419
BssSI CACGAG 2 cut(s) 851, 2303
BssT1I CCWWGG 4 cut(s) 174, 234, 1160, 1188
Bst1107I GTATAC 1 cut(s) 2245
Bst2BI CACGAG 2 cut(s) 851, 2303
Bst2UI CCWGG 6 cut(s) 410, 500, 545, 557, 1701, 2015
Bst6I CTCTTC 2 cut(s) 126, 1763
BstACI GRCGYC 7 cut(s) 372, 447, 1002, 1008, 1245, 1392, 1419
BstBAI YACGTR 1 cut(s) 1403
BstDEI CTNAG 5 cut(s) 229, 343, 397, 1134, 1557
BstDSI CCRYGG 5 cut(s) 174, 234, 379, 1160, 1961
BstF5I GGATG 4 cut(s) 560, 956, 1452, 1849
BstH2I RGCGCY 2 cut(s) 1872, 2003
BstMAI GTCTC 5 cut(s) 50, 230, 1108, 1252, 1926
BstMCI CGRYCG 7 cut(s) 1062, 1116, 1387, 1619, 1629, 1737, 1941
BstNI CCWGG 6 cut(s) 410, 500, 545, 557, 1701, 2015
BstNSI RCATGY 2 cut(s) 26, 2084
BstPAI GACNNNNGTC 2 cut(s) 42, 2222
BstSLI GKGCMC 3 cut(s) 261, 1656, 1743
BstV1I GCAGC 5 cut(s) 405, 676, 1442, 1730, 2243
BstV2I GAAGAC 1 cut(s) 462
BstX2I RGATCY 4 cut(s) 14, 400, 642, 1674
BstYI RGATCY 4 cut(s) 14, 400, 642, 1674
BstZ17I GTATAC 1 cut(s) 2245
BstZI CGGCCG 3 cut(s) 1113, 1616, 1938
BsuI GTATCC 1 cut(s) 869
BtgI CCRYGG 5 cut(s) 174, 234, 379, 1160, 1961
BtrI CACGTC 1 cut(s) 1690
BtsCI GGATG 4 cut(s) 560, 956, 1452, 1849
BtsI GCAGTG 1 cut(s) 1832
BtsIMutI CAGTG 4 cut(s) 671, 1129, 1131, 1832
BtuMI TCGCGA 2 cut(s) 801, 1064
CciI TCATGA 1 cut(s) 1167
Cfr10I RCCGGY 4 cut(s) 1241, 1282, 1624, 2234
Cfr9I CCCGGG 2 cut(s) 2008, 2208
CseI GACGC 9 cut(s) 380, 436, 907, 991, 1234, 1381, 1408, 1943, 2461
Csp6I GTAC 8 cut(s) 105, 310, 486, 1613, 1794, 2068, 2203, 2335
CspAI ACCGGT 2 cut(s) 1282, 1624
CspCI CAANNNNNGTGG 2 cut(s) 1732, 1767
CviQI GTAC 8 cut(s) 105, 310, 486, 1613, 1794, 2068, 2203, 2335
DdeI CTNAG 5 cut(s) 229, 343, 397, 1134, 1557
DrdI GACNNNNNNGTC 2 cut(s) 596, 1391
DriI GACNNNNNGTC 1 cut(s) 1012
DseDI GACNNNNNNGTC 2 cut(s) 596, 1391
EaeI YGGCCR 8 cut(s) 661, 855, 1113, 1146, 1616, 1938, 1978, 2140
EagI CGGCCG 3 cut(s) 1113, 1616, 1938
Eam1104I CTCTTC 2 cut(s) 126, 1763
Eam1105I GACNNNNNGTC 1 cut(s) 1012
EarI CTCTTC 2 cut(s) 126, 1763
EciI GGCGGA 3 cut(s) 304, 413, 774
Ecl136II GAGCTC 2 cut(s) 203, 722
EclXI CGGCCG 3 cut(s) 1113, 1616, 1938
Eco130I CCWWGG 4 cut(s) 174, 234, 1160, 1188
Eco147I AGGCCT 1 cut(s) 1139
Eco24I GRGCYC 5 cut(s) 205, 724, 1656, 1743, 1865
Eco31I GGTCTC 1 cut(s) 50
Eco47I GGWCC 9 cut(s) 406, 846, 1127, 1285, 1308, 1599, 1697, 2134, 2280
Eco47III AGCGCT 1 cut(s) 1870
Eco52I CGGCCG 3 cut(s) 1113, 1616, 1938
Eco53kI GAGCTC 2 cut(s) 203, 722
Eco72I CACGTG 1 cut(s) 1403
Eco88I CYCGRG 5 cut(s) 491, 605, 638, 2008, 2208
EcoICRI GAGCTC 2 cut(s) 203, 722
EcoO109I RGGNCCY 3 cut(s) 186, 846, 1652
EcoRII CCWGG 6 cut(s) 408, 498, 543, 555, 1699, 2013
EcoT14I CCWWGG 4 cut(s) 174, 234, 1160, 1188
EcoT38I GRGCYC 5 cut(s) 205, 724, 1656, 1743, 1865
ErhI CCWWGG 4 cut(s) 174, 234, 1160, 1188
Esp3I CGTCTC 3 cut(s) 1108, 1252, 1926
FaqI GGGAC 4 cut(s) 313, 534, 832, 1612
FauI CCCGC 4 cut(s) 27, 523, 967, 1589
FbaI TGATCA 1 cut(s) 1537
FblI GTMKAC 4 cut(s) 450, 1005, 1986, 2244
FokI GGATG 4 cut(s) 547, 963, 1459, 1836
FriOI GRGCYC 5 cut(s) 205, 724, 1656, 1743, 1865
FspBI CTAG 3 cut(s) 1025, 1220, 1320
GsaI CCCAGC 1 cut(s) 159
GsuI CTGGAG 4 cut(s) 77, 308, 814, 961
HaeII RGCGCY 2 cut(s) 1872, 2003
HgaI GACGC 9 cut(s) 380, 436, 907, 991, 1234, 1381, 1408, 1943, 2461
Hin1I GRCGYC 7 cut(s) 372, 447, 1002, 1008, 1245, 1392, 1419
HincII GTYRAC 4 cut(s) 1006, 1396, 1708, 1987
HindII GTYRAC 4 cut(s) 1006, 1396, 1708, 1987
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 8 cut(s) 284, 341, 347, 434, 515, 522, 608, 671
HphI GGTGA 6 cut(s) 1231, 1387, 1487, 1547, 1614, 2287
HpyAV CCTTC 9 cut(s) 65, 199, 221, 775, 1600, 1990, 2166, 2170, 2277
HpyCH4IV ACGT 4 cut(s) 570, 1008, 1402, 1689
HpyCH4V TGCA 5 cut(s) 259, 702, 2048, 2366, 2447
HpyF3I CTNAG 5 cut(s) 229, 343, 397, 1134, 1557
HpySE526I ACGT 4 cut(s) 570, 1008, 1402, 1689
Hsp92I GRCGYC 7 cut(s) 372, 447, 1002, 1008, 1245, 1392, 1419
KflI GGGWCCC 1 cut(s) 846
Kpn2I TCCGGA 1 cut(s) 403
KroI GCCGGC 1 cut(s) 2234
KroNI GCCGGC 1 cut(s) 2236
Ksp22I TGATCA 1 cut(s) 1537
LguI GCTCTTC 1 cut(s) 1763
Lsp1109I GCAGC 5 cut(s) 405, 676, 1442, 1730, 2243
LweI GCATC 1 cut(s) 569
MaeI CTAG 3 cut(s) 1025, 1220, 1320
MaeII ACGT 4 cut(s) 570, 1008, 1402, 1689
MaeIII GTNAC 9 cut(s) 533, 812, 1322, 1496, 1620, 2110, 2257, 2341, 2437
MauBI CGCGCGCG 1 cut(s) 914
MbiI CCGCTC 3 cut(s) 797, 885, 1040
MboII GAAGA 7 cut(s) 113, 119, 206, 209, 467, 1536, 1780
MfeI CAATTG 1 cut(s) 2397
MflI RGATCY 4 cut(s) 14, 400, 642, 1674
MhlI GDGCHC 8 cut(s) 205, 261, 724, 947, 1656, 1743, 1865, 2060
MlsI TGGCCA 2 cut(s) 857, 1148
MluCI AATT 2 cut(s) 2213, 2397
MluNI TGGCCA 2 cut(s) 857, 1148
MlyI GAGTC 4 cut(s) 335, 428, 516, 617
MmeI TCCRAC 8 cut(s) 363, 456, 588, 1156, 1335, 1501, 1707, 2364
Mox20I TGGCCA 2 cut(s) 857, 1148
MroI TCCGGA 1 cut(s) 403
MroNI GCCGGC 1 cut(s) 2234
MroXI GAANNNNTTC 1 cut(s) 2128
MscI TGGCCA 2 cut(s) 857, 1148
MseI TTAA 1 cut(s) 713
MslI CAYNNNNRTG 5 cut(s) 1082, 1268, 1818, 2053, 2348
Msp20I TGGCCA 2 cut(s) 857, 1148
MspA1I CMGCKG 4 cut(s) 1260, 1433, 1473, 1511
MunI CAATTG 1 cut(s) 2397
Mva1269I GAATGC 2 cut(s) 7, 639
MvaI CCWGG 6 cut(s) 410, 500, 545, 557, 1701, 2015
NaeI GCCGGC 1 cut(s) 2236
NcoI CCATGG 3 cut(s) 174, 234, 1160
NgoMIV GCCGGC 1 cut(s) 2234
NmeAIII GCCGAG 3 cut(s) 439, 1091, 2059
NmuCI GTSAC 6 cut(s) 533, 812, 1496, 1620, 2257, 2341
NruI TCGCGA 2 cut(s) 801, 1064
NspI RCATGY 2 cut(s) 26, 2084
OliI CACNNNNGTG 2 cut(s) 1818, 2348
PaeI GCATGC 1 cut(s) 26
PagI TCATGA 1 cut(s) 1167
PauI GCGCGC 2 cut(s) 914, 1426
PceI AGGCCT 1 cut(s) 1139
PciSI GCTCTTC 1 cut(s) 1763
PcsI WCGNNNNNNNCGW 5 cut(s) 1002, 1005, 1404, 1560, 1599
PctI GAATGC 2 cut(s) 7, 639
PdiI GCCGGC 1 cut(s) 2236
PdmI GAANNNNTTC 1 cut(s) 2128
PfeI GAWTC 4 cut(s) 284, 347, 515, 671
PflFI GACNNNGTC 2 cut(s) 437, 1001
PinAI ACCGGT 2 cut(s) 1282, 1624
Ple19I CGATCG 2 cut(s) 1062, 1737
PleI GAGTC 4 cut(s) 335, 428, 516, 616
PmaCI CACGTG 1 cut(s) 1403
PmlI CACGTG 1 cut(s) 1403
PpsI GAGTC 4 cut(s) 335, 428, 516, 616
Ppu21I YACGTR 1 cut(s) 1403
PpuMI RGGWCCY 1 cut(s) 846
PshAI GACNNNNGTC 2 cut(s) 42, 2222
PsiI TTATAA 1 cut(s) 30
Psp124BI GAGCTC 2 cut(s) 205, 724
Psp5II RGGWCCY 1 cut(s) 846
Psp6I CCWGG 6 cut(s) 408, 498, 543, 555, 1699, 2013
PspCI CACGTG 1 cut(s) 1403
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 6 cut(s) 408, 498, 543, 555, 1699, 2013
PspOMI GGGCCC 2 cut(s) 1652, 1739
PspPPI RGGWCCY 1 cut(s) 846
PsuI RGATCY 4 cut(s) 14, 400, 642, 1674
PsyI GACNNNGTC 2 cut(s) 437, 1001
PteI GCGCGC 2 cut(s) 914, 1426
PvuI CGATCG 2 cut(s) 1062, 1737
PvuII CAGCTG 2 cut(s) 1433, 1511
RruI TCGCGA 2 cut(s) 801, 1064
RsaI GTAC 8 cut(s) 106, 311, 487, 1614, 1795, 2069, 2204, 2336
RsaNI GTAC 8 cut(s) 105, 310, 486, 1613, 1794, 2068, 2203, 2335
RseI CAYNNNNRTG 5 cut(s) 1082, 1268, 1818, 2053, 2348
SacI GAGCTC 2 cut(s) 205, 724
SalI GTCGAC 2 cut(s) 1004, 1985
SapI GCTCTTC 1 cut(s) 1763
SaqAI TTAA 1 cut(s) 713
SchI GAGTC 4 cut(s) 335, 428, 516, 617
SduI GDGCHC 8 cut(s) 205, 261, 724, 947, 1656, 1743, 1865, 2060
SfaNI GCATC 1 cut(s) 569
SfiI GGCCNNNNNGGCC 2 cut(s) 185, 1145
SgrAI CRCCGGYG 1 cut(s) 1241
SgrDI CGTCGACG 1 cut(s) 1004
SinI GGWCC 9 cut(s) 406, 846, 1127, 1285, 1308, 1599, 1697, 2134, 2280
SmaI CCCGGG 2 cut(s) 2010, 2210
SmiMI CAYNNNNRTG 5 cut(s) 1082, 1268, 1818, 2053, 2348
SmlI CTYRAG 4 cut(s) 160, 769, 1751, 1838
SmoI CTYRAG 4 cut(s) 160, 769, 1751, 1838
SphI GCATGC 1 cut(s) 26
Sse9I AATT 2 cut(s) 2213, 2397
SseBI AGGCCT 1 cut(s) 1139
SspMI CTAG 3 cut(s) 1025, 1220, 1320
SstI GAGCTC 2 cut(s) 205, 724
StuI AGGCCT 1 cut(s) 1139
StyI CCWWGG 4 cut(s) 174, 234, 1160, 1188
TaiI ACGT 4 cut(s) 573, 1011, 1405, 1692
TaqII GACCGA 2 cut(s) 32, 1401
TasI AATT 2 cut(s) 2213, 2397
TatI WGTACW 3 cut(s) 104, 2067, 2334
TfiI GAWTC 4 cut(s) 284, 347, 515, 671
Tru1I TTAA 1 cut(s) 713
Tru9I TTAA 1 cut(s) 713
TscAI CASTG 4 cut(s) 671, 1129, 1138, 1839
TseFI GTSAC 6 cut(s) 533, 812, 1496, 1620, 2257, 2341
TseI GCWGC 5 cut(s) 418, 689, 1430, 1718, 2231
Tsp45I GTSAC 6 cut(s) 533, 812, 1496, 1620, 2257, 2341
TspDTI ATGAA 2 cut(s) 113, 278
TspGWI ACGGA 1 cut(s) 2245
TspMI CCCGGG 2 cut(s) 2008, 2208
TspRI CASTG 4 cut(s) 671, 1129, 1138, 1839
Tth111I GACNNNGTC 2 cut(s) 437, 1001
VneI GTGCAC 1 cut(s) 257
VpaK11BI GGWCC 9 cut(s) 406, 846, 1127, 1285, 1308, 1599, 1697, 2134, 2280
XceI RCATGY 2 cut(s) 26, 2084
XmaI CCCGGG 2 cut(s) 2008, 2208
XmiI GTMKAC 4 cut(s) 450, 1005, 1986, 2244
XmnI GAANNNNTTC 1 cut(s) 2128
XspI CTAG 3 cut(s) 1025, 1220, 1320
ZraI GACGTC 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.