Rorug02G0049200

subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013828
Physical Location & Seq
Reverse (-)
3836121 .. 3837466
1346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0049200.1

Sequence Viewer

Length: 1098 bp
ATGATAACGAACACATCACACGGAGGAGAGCAGCAGCACCGCCGGTTGGTTCAGAATCCGAGAGCTTCTTCTTCTTCTTCTTCTTCAGAAAAGCCCATGGAGGACAACGGGAACGTCATCGCGCCGTTCGTTATGAAGACTTATCAGATGGTCAGCGATCCTATAACGGACAGGTTAATCGCTTGGGGGAGGGCTAACAACAGCTTCATCGTCGTCGAGCCTTTGGAGTTCTCGCAGCGGCTGTTGCCGGCTTATTTCAAGCATAATAACTTCTCGAGCTTCGTCCGGCAGCTCAATACTTATGGATTCAGGAAGGTTGATCCGGACAGGTGGGAGTTTGCGAACGAGTGGTTCCTGAGGGGGCAGACGCATTTGTTGAAGAACGTGGTGAGGAAAAAACATAATCACAACAGGACGGTGACGTCGACGGCGTATGGGAAGTCGGAGGAGCTTGACGACGAAGAAATAGTTACGGAGATAGCGAGGCTGAGGCAGGAGCAGAGGAGCTTAGAGGAAGAGGTGGAGAACATGAGCAAGAGATTGGAGGCGACGGAGAGAAGACCGCAGCAGCTGATGGCGTTTCTTCACAGAGTCGCTGAGGATCCCGAGATTTTGCCACGTATGATGCTGGAGAAGGGCCGGAGACTCGCGGAGGGGTTGGGGGACAAGAAGCGTCGGCTGGTGATAGCGTCCACCTCGTCCACGTCATCATCGGGGATGGGGGCGACGGCTTCCGTGAAGACTGAGGATGAAGTTGACGGGGCGATGGGCGTGATTTCGTCGCCGGAGACGGGTTTTGAGATGGAGAGCTTTTCTCGGGGGTCGACGTCGCCGGAGGCGTCGACGGCCCAGGGGTGGTGGAGCCAGAACCGGGTTGTGGGTCTGAATCGGAGTGATAACGAGCTGTATGGTAATTCTACGGGGTTAGCGACGCAGTCGGGTCATGGAAATGGGAATAGTGGTAATTCCGGGTACGGGTTTGGCCCGTTTGGGTATAGTGGAAATAGGAGTGGTAGTGGAGAAGTGGGTTATTTTGCGGAGCTGGAAGAGAGTCCACCACCGCCTTATCCATTCTCACTGTTAGAAGGTGGGTTTTAG

Protein Analysis

365

Amino Acids

40.65

Weight (kDa)

5.86

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 43 - 132 5.1e-28 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05920
fragaria_vesca FvH4_1g08840
malus_domestica MD02G1093500.v1.1 MD15G1218800.v1.1
prunus_persica Prupe.7G197100_v2.0.a1
pyrus_communis pycom02g07370 pycom15g19400
rosa_chinensis RchiOBHm_Chr2g0095311
rosa_laevigata RLG00000016553
rosa_multiflora Rmu_ssc0000183.1_g000049
rosa_roxburghii Rroxscaffold_2G00146490
rosa_rugosa Rorug02G0049200
rosa_samantha Rh2AG097600 Rh2BG098800 Rh2CG100900 Rh2DG098500
rosa_wichuraiana Rw2G007360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 421
AatII GACGTC 2 cut(s) 425, 830
AccI GTMKAC 3 cut(s) 425, 824, 842
AccII CGCG 2 cut(s) 122, 650
AccIII TCCGGA 1 cut(s) 322
AciI CCGC 6 cut(s) 40, 238, 563, 650, 1037, 1061
AclWI GGATC 4 cut(s) 152, 314, 596, 609
AcuI CTGAAG 1 cut(s) 69
AcyI GRCGYC 3 cut(s) 422, 827, 839
AfaI GTAC 1 cut(s) 974
AfiI CCNNNNNNNGG 6 cut(s) 46, 791, 855, 871, 877, 975
AgsI TTSAA 2 cut(s) 259, 379
AjiI CACGTC 1 cut(s) 705
AjnI CCWGG 1 cut(s) 849
Alw26I GTCTC 2 cut(s) 637, 782
AlwI GGATC 4 cut(s) 152, 314, 596, 609
AlwNI CAGNNNCTG 2 cut(s) 241, 571
Ama87I CYCGRG 3 cut(s) 274, 605, 816
Aor13HI TCCGGA 1 cut(s) 322
AoxI GGCC 3 cut(s) 637, 846, 982
ApeKI GCWGC 6 cut(s) 31, 34, 235, 289, 565, 568
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 3 cut(s) 637, 847, 983
AsuC2I CCSGG 2 cut(s) 872, 970
AsuHPI GGTGA 3 cut(s) 400, 430, 694
AvaI CYCGRG 3 cut(s) 274, 605, 816
AxyI CCTNAGG 1 cut(s) 356
BamHI GGATCC 1 cut(s) 601
BbsI GAAGAC 3 cut(s) 143, 565, 746
BbvCI CCTCAGC 2 cut(s) 488, 597
BbvI GCAGC 6 cut(s) 43, 46, 247, 301, 577, 580
BccI CCATC 5 cut(s) 142, 568, 712, 760, 796
BceAI ACGGC 4 cut(s) 109, 444, 744, 861
BciT130I CCWGG 1 cut(s) 851
BcnI CCSGG 2 cut(s) 872, 970
BcoDI GTCTC 2 cut(s) 637, 782
BisI GCNGC 7 cut(s) 32, 35, 236, 239, 290, 566, 569
BlsI GCNGC 7 cut(s) 33, 36, 237, 240, 291, 567, 570
Bme1390I CCNGG 3 cut(s) 851, 872, 970
BmeT110I CYCGRG 3 cut(s) 274, 605, 816
BmgBI CACGTC 1 cut(s) 705
BmgT120I GGNCC 3 cut(s) 637, 847, 983
BmiI GGNNCC 3 cut(s) 353, 603, 863
BmrFI CCNGG 3 cut(s) 851, 872, 970
BmsI GCATC 1 cut(s) 615
BpiI GAAGAC 3 cut(s) 143, 565, 746
BplI GAGNNNNNCTC 2 cut(s) 799, 831
BpmI CTGGAG 1 cut(s) 650
Bpu10I CCTNAGC 2 cut(s) 488, 597
BpuMI CCSGG 2 cut(s) 872, 970
BsaAI YACGTR 1 cut(s) 620
BsaHI GRCGYC 3 cut(s) 422, 827, 839
BsaJI CCNNGG 3 cut(s) 96, 849, 850
BsaWI WCCGGW 1 cut(s) 322
BsaXI ACNNNNNCTCC 2 cut(s) 1000, 1030
Bsc4I CCNNNNNNNGG 6 cut(s) 46, 791, 855, 871, 877, 975
Bse118I RCCGGY 2 cut(s) 42, 247
Bse21I CCTNAGG 1 cut(s) 356
BseAI TCCGGA 1 cut(s) 322
BseBI CCWGG 1 cut(s) 851
BseDI CCNNGG 3 cut(s) 96, 849, 850
BseGI GGATG 2 cut(s) 723, 754
BseLI CCNNNNNNNGG 6 cut(s) 46, 791, 855, 871, 877, 975
BseMII CTCAG 4 cut(s) 347, 479, 588, 735
BseRI GAGGAG 3 cut(s) 39, 461, 517
BseXI GCAGC 6 cut(s) 43, 46, 247, 301, 577, 580
Bsh1236I CGCG 2 cut(s) 122, 650
BshFI GGCC 3 cut(s) 639, 848, 984
BsiHKCI CYCGRG 3 cut(s) 274, 605, 816
BsiSI CCGG 9 cut(s) 43, 248, 286, 323, 640, 785, 833, 871, 969
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 6 cut(s) 46, 791, 855, 871, 877, 975
BsmAI GTCTC 2 cut(s) 637, 782
BsmBI CGTCTC 1 cut(s) 782
BsmFI GGGAC 1 cut(s) 677
BsnI GGCC 3 cut(s) 639, 848, 984
BsoBI CYCGRG 3 cut(s) 274, 605, 816
Bsp13I TCCGGA 1 cut(s) 322
Bsp143I GATC 3 cut(s) 157, 319, 601
Bsp19I CCATGG 1 cut(s) 96
BspACI CCGC 6 cut(s) 40, 238, 563, 650, 1037, 1061
BspANI GGCC 3 cut(s) 639, 848, 984
BspCNI CTCAG 4 cut(s) 348, 480, 589, 736
BspEI TCCGGA 1 cut(s) 322
BspFNI CGCG 2 cut(s) 122, 650
BspLI GGNNCC 3 cut(s) 353, 603, 863
BspPI GGATC 4 cut(s) 152, 314, 596, 609
BsrFI RCCGGY 2 cut(s) 42, 247
BssAI RCCGGY 2 cut(s) 42, 247
BssECI CCNNGG 3 cut(s) 96, 849, 850
BssMI GATC 3 cut(s) 157, 319, 601
BssNI GRCGYC 3 cut(s) 422, 827, 839
BssT1I CCWWGG 1 cut(s) 96
Bst2UI CCWGG 1 cut(s) 851
Bst4CI ACNGT 2 cut(s) 418, 1080
Bst6I CTCTTC 2 cut(s) 510, 1041
BstACI GRCGYC 3 cut(s) 422, 827, 839
BstBAI YACGTR 1 cut(s) 620
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 5 cut(s) 356, 488, 508, 597, 744
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 2 cut(s) 723, 754
BstFNI CGCG 2 cut(s) 122, 650
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 3 cut(s) 160, 322, 604
BstMAI GTCTC 2 cut(s) 637, 782
BstMBI GATC 3 cut(s) 157, 319, 601
BstMWI GCNNNNNNNGC 2 cut(s) 244, 845
BstNI CCWGG 1 cut(s) 851
BstSCI CCNGG 3 cut(s) 849, 870, 968
BstUI CGCG 2 cut(s) 122, 650
BstV1I GCAGC 6 cut(s) 43, 46, 247, 301, 577, 580
BstV2I GAAGAC 3 cut(s) 143, 565, 746
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
Bsu36I CCTNAGG 1 cut(s) 356
BsuRI GGCC 3 cut(s) 639, 848, 984
BtgI CCRYGG 1 cut(s) 96
BtgZI GCGATG 2 cut(s) 103, 779
BtrI CACGTC 1 cut(s) 705
BtsCI GGATG 2 cut(s) 723, 754
BtsIMutI CAGTG 1 cut(s) 1076
Cac8I GCNNGC 1 cut(s) 249
CaiI CAGNNNCTG 2 cut(s) 241, 571
CfoI GCGC 1 cut(s) 124
Cfr10I RCCGGY 2 cut(s) 42, 247
Cfr13I GGNCC 3 cut(s) 637, 847, 983
CseI GACGC 5 cut(s) 376, 662, 678, 828, 940
Csp6I GTAC 1 cut(s) 973
CviAII CATG 3 cut(s) 97, 529, 944
CviQI GTAC 1 cut(s) 973
DdeI CTNAG 5 cut(s) 356, 488, 508, 597, 744
DpnI GATC 3 cut(s) 159, 321, 603
DpnII GATC 3 cut(s) 157, 319, 601
DrdI GACNNNNNNGTC 1 cut(s) 421
DseDI GACNNNNNNGTC 1 cut(s) 421
Eam1104I CTCTTC 2 cut(s) 510, 1041
EarI CTCTTC 2 cut(s) 510, 1041
Eco130I CCWWGG 1 cut(s) 96
Eco57I CTGAAG 1 cut(s) 69
Eco81I CCTNAGG 1 cut(s) 356
Eco88I CYCGRG 3 cut(s) 274, 605, 816
EcoRII CCWGG 1 cut(s) 849
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
Esp3I CGTCTC 1 cut(s) 782
FaeI CATG 3 cut(s) 100, 532, 947
FaqI GGGAC 1 cut(s) 677
FatI CATG 3 cut(s) 96, 528, 943
FblI GTMKAC 3 cut(s) 425, 824, 842
Fnu4HI GCNGC 7 cut(s) 32, 35, 236, 239, 290, 566, 569
FokI GGATG 2 cut(s) 730, 761
Fsp4HI GCNGC 7 cut(s) 32, 35, 236, 239, 290, 566, 569
GlaI GCGC 1 cut(s) 123
GluI GCNGC 7 cut(s) 32, 35, 236, 239, 290, 566, 569
GsuI CTGGAG 1 cut(s) 650
HaeIII GGCC 3 cut(s) 639, 848, 984
HapII CCGG 9 cut(s) 43, 248, 286, 323, 640, 785, 833, 871, 969
HgaI GACGC 5 cut(s) 376, 662, 678, 828, 940
HhaI GCGC 1 cut(s) 124
Hin1I GRCGYC 3 cut(s) 422, 827, 839
Hin1II CATG 3 cut(s) 100, 532, 947
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HincII GTYRAC 4 cut(s) 426, 757, 825, 843
HindII GTYRAC 4 cut(s) 426, 757, 825, 843
HinfI GANTC 6 cut(s) 55, 306, 591, 645, 886, 1051
HpaII CCGG 9 cut(s) 43, 248, 286, 323, 640, 785, 833, 871, 969
HphI GGTGA 3 cut(s) 400, 430, 694
Hpy166II GTNNAC 7 cut(s) 426, 693, 702, 757, 825, 843, 1055
Hpy188I TCNGA 7 cut(s) 54, 60, 88, 147, 445, 885, 891
Hpy188III TCNNGA 5 cut(s) 274, 310, 323, 355, 605
Hpy8I GTNNAC 7 cut(s) 426, 693, 702, 757, 825, 843, 1055
HpyAV CCTTC 3 cut(s) 307, 628, 1079
HpyCH4III ACNGT 2 cut(s) 418, 1080
HpyCH4IV ACGT 6 cut(s) 114, 384, 422, 619, 704, 827
HpyF10VI GCNNNNNNNGC 2 cut(s) 244, 845
HpyF3I CTNAG 5 cut(s) 356, 488, 508, 597, 744
HpySE526I ACGT 6 cut(s) 114, 384, 422, 619, 704, 827
Hsp92I GRCGYC 3 cut(s) 422, 827, 839
Hsp92II CATG 3 cut(s) 100, 532, 947
HspAI GCGC 1 cut(s) 122
Kpn2I TCCGGA 1 cut(s) 322
KroI GCCGGC 1 cut(s) 247
KroNI GCCGGC 1 cut(s) 249
Kzo9I GATC 3 cut(s) 157, 319, 601
LmnI GCTCC 5 cut(s) 448, 496, 504, 861, 1039
Lsp1109I GCAGC 6 cut(s) 43, 46, 247, 301, 577, 580
LweI GCATC 1 cut(s) 615
MaeII ACGT 6 cut(s) 114, 384, 422, 619, 704, 827
MaeIII GTNAC 2 cut(s) 418, 469
MalI GATC 3 cut(s) 159, 321, 603
MboI GATC 3 cut(s) 157, 319, 601
MflI RGATCY 1 cut(s) 601
MluCI AATT 2 cut(s) 913, 964
MlyI GAGTC 3 cut(s) 600, 639, 1060
MmeI TCCRAC 1 cut(s) 423
MroI TCCGGA 1 cut(s) 322
MroNI GCCGGC 1 cut(s) 247
MseI TTAA 1 cut(s) 176
MslI CAYNNNNRTG 1 cut(s) 948
MspA1I CMGCKG 2 cut(s) 238, 571
MspI CCGG 9 cut(s) 43, 248, 286, 323, 640, 785, 833, 871, 969
MspR9I CCNGG 3 cut(s) 851, 872, 970
MvaI CCWGG 1 cut(s) 851
MvnI CGCG 2 cut(s) 122, 650
MwoI GCNNNNNNNGC 2 cut(s) 244, 845
NaeI GCCGGC 1 cut(s) 249
NciI CCSGG 2 cut(s) 872, 970
NcoI CCATGG 1 cut(s) 96
NdeII GATC 3 cut(s) 157, 319, 601
NgoMIV GCCGGC 1 cut(s) 247
NlaIII CATG 3 cut(s) 100, 532, 947
NlaIV GGNNCC 3 cut(s) 353, 603, 863
NmuCI GTSAC 1 cut(s) 418
PaeR7I CTCGAG 1 cut(s) 274
PasI CCCWGGG 1 cut(s) 850
PcsI WCGNNNNNNNCGW 6 cut(s) 126, 422, 428, 479, 836, 926
PdiI GCCGGC 1 cut(s) 249
PfeI GAWTC 3 cut(s) 55, 306, 886
PflFI GACNNNGTC 1 cut(s) 934
PkrI GCNGC 7 cut(s) 33, 36, 237, 240, 291, 567, 570
PleI GAGTC 3 cut(s) 599, 639, 1059
PpsI GAGTC 3 cut(s) 599, 639, 1059
Ppu21I YACGTR 1 cut(s) 620
Psp6I CCWGG 1 cut(s) 849
PspGI CCWGG 1 cut(s) 849
PspN4I GGNNCC 3 cut(s) 353, 603, 863
PspPI GGNCC 3 cut(s) 637, 847, 983
PstNI CAGNNNCTG 2 cut(s) 241, 571
PsuI RGATCY 1 cut(s) 601
PsyI GACNNNGTC 1 cut(s) 934
PvuII CAGCTG 1 cut(s) 571
RsaI GTAC 1 cut(s) 974
RsaNI GTAC 1 cut(s) 973
RseI CAYNNNNRTG 1 cut(s) 948
SalI GTCGAC 3 cut(s) 424, 823, 841
SaqAI TTAA 1 cut(s) 176
SatI GCNGC 7 cut(s) 32, 35, 236, 239, 290, 566, 569
Sau3AI GATC 3 cut(s) 157, 319, 601
Sau96I GGNCC 3 cut(s) 637, 847, 983
SchI GAGTC 3 cut(s) 600, 639, 1060
ScrFI CCNGG 3 cut(s) 851, 872, 970
SfaNI GCATC 1 cut(s) 615
Sfr274I CTCGAG 1 cut(s) 274
SgrDI CGTCGACG 2 cut(s) 424, 841
SlaI CTCGAG 1 cut(s) 274
SmiMI CAYNNNNRTG 1 cut(s) 948
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 2 cut(s) 913, 964
SsiI CCGC 6 cut(s) 40, 238, 563, 650, 1037, 1061
StyD4I CCNGG 3 cut(s) 849, 870, 968
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 2 cut(s) 418, 1080
TaiI ACGT 6 cut(s) 117, 387, 425, 622, 707, 830
TaqI TCGA 5 cut(s) 216, 275, 425, 824, 842
TasI AATT 2 cut(s) 913, 964
TauI GCSGC 1 cut(s) 241
TfiI GAWTC 3 cut(s) 55, 306, 886
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TscAI CASTG 1 cut(s) 1083
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 6 cut(s) 31, 34, 235, 289, 565, 568
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 3 cut(s) 149, 196, 765
TspGWI ACGGA 5 cut(s) 36, 182, 488, 566, 724
TspRI CASTG 1 cut(s) 1083
Tth111I GACNNNGTC 1 cut(s) 934
XhoI CTCGAG 1 cut(s) 274
XmiI GTMKAC 3 cut(s) 425, 824, 842
ZraI GACGTC 2 cut(s) 423, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.