Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0013828

Physical Location & Seq

Genomic Coordinates Forward (+)

17695889 .. 17698699

Gene Structure 2811 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1218800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2445 bp

ATGCTTTTAAACCCGACAACGTACTCTTTCTCCTTCTACCTTCTTTTATCCCTTTCTGACAGTCGCAGCTCCAGAGCGACTGTACTCTCACTCTCTCTTCATCTTCTACATTTGGATATACGAAATCTACCCAGCTCAAGCTTTCTAGTCATGGCCGCCGAGGCCTTCTGGTTCGGAGTTCTGCTTCTTCTTCTCCCATGTTTCTCAGCCATGGCGAAGCAGACTTACATTGTGCAGATGAACCACCACTCCAAGCCGGAATCCTACGCCACCCACCACGACTGGTACTCCGCCAGCCTCCAATCCCTCTCCTCCGACTCAGATTCCCTCCTTTACACCTACACCGATGCCTACCATGGCTTCGCCGCCTCCTTAGATCCGGAGCAGGCCGAGCTGCTCCACCAGTCCGACTCCGTCATCGGCGTTTACGAAGACACGGTTTACAACCTTCACACCACTCGGACCCCCGAGTTTCTCGGTCTCGATACCGAATCGGGGCTATGGGAGGGTCACTCGACCCAGGATTTGAACCAGGCCTCCAATGACGTCATCGTCGGAGTTCTCGACACCGGGGTCTGGCCCGAGTCCAAGAGCTTCGACGACACCGGAATGCCCGAGATCCCTACCCGGTGGCGCGGCCAGTGCGAATCGGGGCCCGATTTCGCCGCAACGCTTTGCAACAAGAAGCTAATCGGAGCTCGGAGCTTCTCGAAAGGCTACCACATGGCCTCCGGTGGGAGCTACTTGAGAAAACCGAGGGAAGTCGAGTCCCCGCGCGACAGAGACGGTCACGGTACCCACACCTCCAGCACCGCGGCAGGGTCCCTCGTGGCCAACGCCAGCCTCCTCGGATACGCGACGGGAACGGCTCGCGGAATGGCCCCTCACGCGCGCGTCGCCACCTACAAGGTCTGCTGGAGCACCGGGTGTTTCGGGTCAGACATTCTCGCTGGCATGGATCGGGCCATCGTTGACGGCGTCGACGTCATGTCACTCTCCCTTGGCGGTGGCTCCGCTCCCTACTACCGCGACACGATCGCGATTGGCGCGTTCACGGCTATGGAAAGGGGTATTTTCGTCTCTTGCTCTGCCGGGAACAGTGGGCCCACTAGAGCCTCGTTGGCCAACACAGCCCCCTGGATCATGACCGTTGGAGCCGGGACCTTGGACCGGGATTTCCCGGCTTACGCTTTGTTAGGCAACAAACTCCCGTTCACCGGCGTTTCGCTTTACAGCGGGACCGGAATGGGAAACAAACCGGTCCAGTTGGTTTACAACAGGGGGTCCAACAGCTCTGGTAACTTGTGCCTGCCCGGTTCGCTCAACCCGGATTTGGTTCGTGGGAAAGTGGTGATGTGCGACCGAGGCATCAACGCACGTGTCGAGAAAGGTGGCGTCGTGCGCTCAGCTGGGGGGATTGGGATGATACTCGCCAACACCGCCGCCAGCGGTGAGGAGTTGGTGGCTGACAGTCATTTGCTACCTGCAGTCGCCGTCGGGAGACGAGTCGGTGACCAAATCAGGGAATATGCTCAGCACGATCCCAATCCGACGGCTGTGATTACTTTCGGCAGGACTGTGCTGAATGTACGGCCGTCACCGGTCGTGGCGGCGTTTAGTTCACGGGGGCCCAATTTGGTGAACCCCCAGATCTTGAAGCCGGACGTGATTGGTCCAGGTGTCAATATTTTGGCGGCATGGTCAGAGGCAGTCGGGCTCACGGGGCTTGAGGAGGACAAGAGGAAGAGCCAGTTCAACATCATTTCAGGTACATCAATGTCATGCCCACACATTATTGGGCTTGCAGCATTGCTTAAGGCGGCCCATCCGGAATGGAGCCCAAGTGCTGTGAAATCTGCCCTAATGACCACAGCTTACACCCATGACAACACCAAGGCCCCTCTCCGAGACGCGGCCGATGGCACGATTTCGAACCCGTGGGCTCACGGCTCCGGGCACATCGACCCCTCGAAGGCGCTGTCACCGGGCTTGGTTTACGACATTGCCACCGAGGACTACATTGCATTTTTGTGCTCATTGGAGTACACCAAGGAGCATGTCCAAGCCATTGTCAAGAGGCCCAATGTTACTTGTGCACGAAAGTATTCGGACCCGGGCCAGCTCAACTACCCTTCGTTCTCAGTCGTGTTCTGGAACAAAAAGAAAAGGGTTGTGAGTTACACCCGGGAATTGACGAATGTCGGGCCTGCCGGATCTGTATACAGAGTCGCCGTAACCAGTCCTTCGATGGTGAGGACCATCGTGAAGCCCACAAGGCTTGTGTTCAACAATGTAGGAGAGAAGCAGAAGTACAGAGTCACATTTGTGGCTTCGCGGGGTGCAGAGAAAACATCAAGGTCGGAATTCGGGTCAATCATGTGGTCAAACCCACAACACCAAGTGAAAAGCCCAGTTGCTTTTGCATGGACGCAGTTGATAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005911 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009505 GO:0009506 GO:0012505 GO:0016787 GO:0017171 GO:0019538 GO:0030054 GO:0030312 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0070011 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

815

Amino Acids

87.6

Weight (kDa)

7.93

Isoelectric Point (pI)

35.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	75 - 151	6.6e-18	Peptidase inhibitor I9
Peptidase_S8	PF00082	181 - 631	7.2e-44	Subtilase family
PA	PF02225	423 - 507	5.4e-11	PA domain
fn3_6	PF17766	707 - 806	3.2e-26	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05920
fragaria_vesca	FvH4_1g08840
malus_domestica	MD02G1093500.v1.1 MD15G1218800.v1.1
prunus_persica	Prupe.7G197100_v2.0.a1
pyrus_communis	pycom02g07370 pycom15g19400
rosa_chinensis	RchiOBHm_Chr2g0095311
rosa_laevigata	RLG00000016553
rosa_multiflora	Rmu_ssc0000183.1_g000049
rosa_roxburghii	Rroxscaffold_2G00146490
rosa_rugosa	Rorug02G0049200
rosa_samantha	Rh2AG097600 Rh2BG098800 Rh2CG100900 Rh2DG098500
rosa_wichuraiana	Rw2G007360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	551, 572
AatII	GACGTC	2 cut(s)	549, 987
Acc36I	ACCTGC	1 cut(s)	1492
Acc65I	GGTACC	1 cut(s)	794
AccB1I	GGYRCC	1 cut(s)	794
AccBSI	CCGCTC	1 cut(s)	1016
AccI	GTMKAC	2 cut(s)	981, 2220
AccIII	TCCGGA	2 cut(s)	379, 1828
AclWI	GGATC	6 cut(s)	371, 613, 966, 1148, 1535, 2221
AcoI	YGGCCR	6 cut(s)	153, 637, 831, 1122, 1592, 1914
AcsI	RAATTY	1 cut(s)	2363
AcvI	CACGTG	1 cut(s)	1379
AcyI	GRCGYC	4 cut(s)	546, 978, 984, 1395
AdeI	CACNNNGTG	2 cut(s)	927, 2401
AfaI	GTAC	8 cut(s)	23, 84, 287, 796, 1590, 1771, 2045, 2312
AflII	CTTAAG	1 cut(s)	1814
AflIII	ACRYGT	1 cut(s)	1378
AgeI	ACCGGT	2 cut(s)	1258, 1600
AgsI	TTSAA	4 cut(s)	529, 1657, 1756, 2287
AhdI	GACNNNNNGTC	1 cut(s)	988
AjiI	CACGTC	1 cut(s)	1666
AjnI	CCWGG	4 cut(s)	519, 531, 1136, 1675
AleI	CACNNNNGTG	1 cut(s)	2324
AloI	GAACNNNNNNTCC	4 cut(s)	233, 265, 521, 553
Alw21I	GWGCWC	4 cut(s)	700, 923, 2036, 2098
Alw26I	GTCTC	5 cut(s)	485, 777, 1084, 1495, 1902
Alw44I	GTGCAC	1 cut(s)	2094
AlwI	GGATC	6 cut(s)	371, 613, 966, 1148, 1535, 2221
Ama87I	CYCGRG	5 cut(s)	467, 581, 614, 2113, 2184
Aor13HI	TCCGGA	2 cut(s)	379, 1828
ApaI	GGGCCC	3 cut(s)	657, 1107, 1632
ApaLI	GTGCAC	1 cut(s)	2094
ApeKI	GCWGC	3 cut(s)	66, 394, 1805
ApoI	RAATTY	1 cut(s)	2363
ArsI	GACNNNNNNTTYG	4 cut(s)	508, 540, 1964, 1996
AsiGI	ACCGGT	2 cut(s)	1258, 1600
Asp700I	GAANNNNTTC	1 cut(s)	2104
Asp718I	GGTACC	1 cut(s)	794
AspLEI	GCGC	7 cut(s)	636, 777, 892, 894, 1049, 1404, 1978
AsuHPI	GGTGA	8 cut(s)	1207, 1363, 1463, 1523, 1590, 1651, 1974, 2263
AsuII	TTCGAA	1 cut(s)	1931
AvaI	CYCGRG	5 cut(s)	467, 581, 614, 2113, 2184
BaeGI	GKGCMC	5 cut(s)	657, 1107, 1632, 1959, 2098
BalI	TGGCCA	2 cut(s)	833, 1124
BanI	GGYRCC	1 cut(s)	794
BanII	GRGCYC	7 cut(s)	657, 700, 1107, 1632, 1719, 1841, 1945
BarI	GAAGNNNNNNTAC	4 cut(s)	209, 241, 2294, 2326
BauI	CACGAG	1 cut(s)	827
BbrPI	CACGTG	1 cut(s)	1379
BbsI	GAAGAC	1 cut(s)	438
Bbv12I	GWGCWC	4 cut(s)	700, 923, 2036, 2098
BbvI	GCAGC	3 cut(s)	78, 381, 1817
BccI	CCATC	5 cut(s)	974, 1833, 1913, 2242, 2267
BceAI	ACGGC	9 cut(s)	882, 991, 1071, 1478, 1569, 1579, 1607, 1963, 2216
BciT130I	CCWGG	4 cut(s)	521, 533, 1138, 1677
BciVI	GTATCC	1 cut(s)	845
BcoDI	GTCTC	5 cut(s)	485, 777, 1084, 1495, 1902
BfaI	CTAG	2 cut(s)	146, 1110
BfmI	CTRYAG	1 cut(s)	1485
BfoI	RGCGCY	1 cut(s)	1979
BfrI	CTTAAG	1 cut(s)	1814
BfuAI	ACCTGC	1 cut(s)	1492
BfuI	GTATCC	1 cut(s)	845
BglI	GCCNNNNNGGC	3 cut(s)	161, 1121, 2275
BglII	AGATCT	1 cut(s)	1650
BlpI	GCTNAGC	2 cut(s)	1405, 1533
BmeRI	GACNNNNNGTC	1 cut(s)	988
BmeT110I	CYCGRG	5 cut(s)	467, 581, 614, 2113, 2184
BmgBI	CACGTC	1 cut(s)	1666
BmrI	ACTGGG	1 cut(s)	2405
BmsI	GCATC	2 cut(s)	337, 1377
BmuI	ACTGGG	1 cut(s)	2405
BoxI	GACNNNNGTC	1 cut(s)	2198
BpiI	GAAGAC	1 cut(s)	438
BplI	GAGNNNNNCTC	2 cut(s)	497, 529
BpmI	CTGGAG	3 cut(s)	55, 790, 937
Bpu1102I	GCTNAGC	2 cut(s)	1405, 1533
Bpu14I	TTCGAA	1 cut(s)	1931
BpuEI	CTTGAG	3 cut(s)	121, 766, 1748
BsaAI	YACGTR	1 cut(s)	1379
BsaBI	GATNNNNATC	1 cut(s)	1545
BsaHI	GRCGYC	4 cut(s)	546, 978, 984, 1395
BsaI	GGTCTC	1 cut(s)	485
BsaWI	WCCGGW	7 cut(s)	379, 605, 731, 1241, 1258, 1600, 1828
Bse118I	RCCGGY	3 cut(s)	1217, 1258, 1600
Bse1I	ACTGG	7 cut(s)	287, 403, 640, 1264, 1750, 2238, 2411
Bse3DI	GCAATG	3 cut(s)	1808, 2001, 2019
Bse8I	GATNNNNATC	1 cut(s)	1545
BseAI	TCCGGA	2 cut(s)	379, 1828
BseBI	CCWGG	4 cut(s)	521, 533, 1138, 1677
BseGI	GGATG	2 cut(s)	1428, 1825
BseJI	GATNNNNATC	1 cut(s)	1545
BseMI	GCAATG	3 cut(s)	1808, 2001, 2019
BseMII	CTCAG	5 cut(s)	219, 333, 1419, 1547, 2154
BseNI	ACTGG	7 cut(s)	287, 403, 640, 1264, 1750, 2238, 2411
BsePI	GCGCGC	1 cut(s)	890
BseRI	GAGGAG	4 cut(s)	301, 836, 1469, 1745
BseSI	GKGCMC	5 cut(s)	657, 1107, 1632, 1959, 2098
BseX3I	CGGCCG	2 cut(s)	1592, 1914
BseXI	GCAGC	3 cut(s)	78, 381, 1817
BseYI	CCCAGC	2 cut(s)	131, 1409
BsgI	GTGCAG	2 cut(s)	254, 2361
Bsh1285I	CGRYCG	5 cut(s)	1038, 1363, 1595, 1605, 1917
BshNI	GGYRCC	1 cut(s)	794
BshTI	ACCGGT	2 cut(s)	1258, 1600
BsiEI	CGRYCG	5 cut(s)	1038, 1363, 1595, 1605, 1917
BsiHKAI	GWGCWC	4 cut(s)	700, 923, 2036, 2098
BsiHKCI	CYCGRG	5 cut(s)	467, 581, 614, 2113, 2184
BslFI	GGGAC	4 cut(s)	754, 808, 1174, 1252
BsmAI	GTCTC	5 cut(s)	485, 777, 1084, 1495, 1902
BsmBI	CGTCTC	4 cut(s)	777, 1084, 1495, 1902
BsmFI	GGGAC	4 cut(s)	754, 808, 1174, 1252
BsmI	GAATGC	1 cut(s)	615
Bso31I	GGTCTC	1 cut(s)	485
BsoBI	CYCGRG	5 cut(s)	467, 581, 614, 2113, 2184
Bsp119I	TTCGAA	1 cut(s)	1931
Bsp120I	GGGCCC	3 cut(s)	653, 1103, 1628
Bsp13I	TCCGGA	2 cut(s)	379, 1828
Bsp143I	GATC	8 cut(s)	376, 618, 958, 1035, 1140, 1540, 1650, 2213
Bsp1720I	GCTNAGC	2 cut(s)	1405, 1533
Bsp19I	CCATGG	2 cut(s)	210, 355
Bsp68I	TCGCGA	1 cut(s)	1040
BspCNI	CTCAG	5 cut(s)	218, 332, 1418, 1546, 2153
BspEI	TCCGGA	2 cut(s)	379, 1828
BspHI	TCATGA	1 cut(s)	1143
BspMAI	CTGCAG	1 cut(s)	1489
BspMI	ACCTGC	1 cut(s)	1492
BspPI	GGATC	6 cut(s)	371, 613, 966, 1148, 1535, 2221
BspQI	GCTCTTC	1 cut(s)	1739
BspT104I	TTCGAA	1 cut(s)	1931
BspT107I	GGYRCC	1 cut(s)	794
BspTI	CTTAAG	1 cut(s)	1814
BspTNI	GGTCTC	1 cut(s)	485
BsrBI	CCGCTC	1 cut(s)	1016
BsrDI	GCAATG	3 cut(s)	1808, 2001, 2019
BsrFI	RCCGGY	3 cut(s)	1217, 1258, 1600
BsrI	ACTGG	7 cut(s)	287, 403, 640, 1264, 1750, 2238, 2411
BssAI	RCCGGY	3 cut(s)	1217, 1258, 1600
BssHII	GCGCGC	1 cut(s)	890
BssMI	GATC	8 cut(s)	376, 618, 958, 1035, 1140, 1540, 1650, 2213
BssNAI	GTATAC	1 cut(s)	2221
BssNI	GRCGYC	4 cut(s)	546, 978, 984, 1395
BssSI	CACGAG	1 cut(s)	827
BssT1I	CCWWGG	6 cut(s)	210, 355, 1000, 1164, 1893, 2049
Bst1107I	GTATAC	1 cut(s)	2221
Bst2BI	CACGAG	1 cut(s)	827
Bst2UI	CCWGG	4 cut(s)	521, 533, 1138, 1677
Bst4CI	ACNGT	9 cut(s)	62, 82, 439, 788, 794, 1100, 1150, 1472, 1579
Bst6I	CTCTTC	2 cut(s)	102, 1739
BstACI	GRCGYC	4 cut(s)	546, 978, 984, 1395
BstAFI	CTTAAG	1 cut(s)	1814
BstBAI	YACGTR	1 cut(s)	1379
BstBI	TTCGAA	1 cut(s)	1931
BstDEI	CTNAG	6 cut(s)	205, 319, 373, 1405, 1533, 2140
BstDSI	CCRYGG	4 cut(s)	210, 355, 813, 1937
BstEII	GGTNACC	1 cut(s)	1511
BstF5I	GGATG	2 cut(s)	1428, 1825
BstH2I	RGCGCY	1 cut(s)	1979
BstHHI	GCGC	7 cut(s)	636, 777, 892, 894, 1049, 1404, 1978
BstKTI	GATC	8 cut(s)	379, 621, 961, 1038, 1143, 1543, 1653, 2216
BstMAI	GTCTC	5 cut(s)	485, 777, 1084, 1495, 1902
BstMBI	GATC	8 cut(s)	376, 618, 958, 1035, 1140, 1540, 1650, 2213
BstMCI	CGRYCG	5 cut(s)	1038, 1363, 1595, 1605, 1917
BstNI	CCWGG	4 cut(s)	521, 533, 1138, 1677
BstNSI	RCATGY	1 cut(s)	2060
BstPAI	GACNNNNGTC	1 cut(s)	2198
BstPI	GGTNACC	1 cut(s)	1511
BstSFI	CTRYAG	1 cut(s)	1485
BstSLI	GKGCMC	5 cut(s)	657, 1107, 1632, 1959, 2098
BstV1I	GCAGC	3 cut(s)	78, 381, 1817
BstV2I	GAAGAC	1 cut(s)	438
BstX2I	RGATCY	4 cut(s)	376, 618, 1650, 2213
BstYI	RGATCY	4 cut(s)	376, 618, 1650, 2213
BstZ17I	GTATAC	1 cut(s)	2221
BstZI	CGGCCG	2 cut(s)	1592, 1914
BsuI	GTATCC	1 cut(s)	845
BtgI	CCRYGG	4 cut(s)	210, 355, 813, 1937
BtrI	CACGTC	1 cut(s)	1666
BtsCI	GGATG	2 cut(s)	1428, 1825
BtsIMutI	CAGTG	2 cut(s)	647, 1105
BtuMI	TCGCGA	1 cut(s)	1040
BveI	ACCTGC	1 cut(s)	1492
CciI	TCATGA	1 cut(s)	1143
CfoI	GCGC	7 cut(s)	636, 777, 892, 894, 1049, 1404, 1978
Cfr10I	RCCGGY	3 cut(s)	1217, 1258, 1600
Cfr42I	CCGCGG	1 cut(s)	816
Cfr9I	CCCGGG	2 cut(s)	2113, 2184
CseI	GACGC	5 cut(s)	883, 967, 1384, 1919, 2437
Csp6I	GTAC	8 cut(s)	22, 83, 286, 795, 1589, 1770, 2044, 2311
CspAI	ACCGGT	2 cut(s)	1258, 1600
CspCI	CAANNNNNGTGG	2 cut(s)	2260, 2295
CviQI	GTAC	8 cut(s)	22, 83, 286, 795, 1589, 1770, 2044, 2311
DdeI	CTNAG	6 cut(s)	205, 319, 373, 1405, 1533, 2140
DpnI	GATC	8 cut(s)	378, 620, 960, 1037, 1142, 1542, 1652, 2215
DpnII	GATC	8 cut(s)	376, 618, 958, 1035, 1140, 1540, 1650, 2213
DraI	TTTAAA	1 cut(s)	9
DraIII	CACNNNGTG	2 cut(s)	927, 2401
DrdI	GACNNNNNNGTC	2 cut(s)	551, 572
DriI	GACNNNNNGTC	1 cut(s)	988
DseDI	GACNNNNNNGTC	2 cut(s)	551, 572
EaeI	YGGCCR	6 cut(s)	153, 637, 831, 1122, 1592, 1914
EagI	CGGCCG	2 cut(s)	1592, 1914
Eam1104I	CTCTTC	2 cut(s)	102, 1739
Eam1105I	GACNNNNNGTC	1 cut(s)	988
EarI	CTCTTC	2 cut(s)	102, 1739
EciI	GGCGGA	1 cut(s)	280
Ecl136II	GAGCTC	1 cut(s)	698
EclXI	CGGCCG	2 cut(s)	1592, 1914
Eco130I	CCWWGG	6 cut(s)	210, 355, 1000, 1164, 1893, 2049
Eco147I	AGGCCT	2 cut(s)	164, 536
Eco24I	GRGCYC	7 cut(s)	657, 700, 1107, 1632, 1719, 1841, 1945
Eco31I	GGTCTC	1 cut(s)	485
Eco52I	CGGCCG	2 cut(s)	1592, 1914
Eco53kI	GAGCTC	1 cut(s)	698
Eco72I	CACGTG	1 cut(s)	1379
Eco88I	CYCGRG	5 cut(s)	467, 581, 614, 2113, 2184
Eco91I	GGTNACC	1 cut(s)	1511
EcoICRI	GAGCTC	1 cut(s)	698
EcoO109I	RGGNCCY	5 cut(s)	653, 822, 1161, 1628, 1897
EcoO65I	GGTNACC	1 cut(s)	1511
EcoRI	GAATTC	1 cut(s)	2363
EcoRII	CCWGG	4 cut(s)	519, 531, 1136, 1675
EcoT14I	CCWWGG	6 cut(s)	210, 355, 1000, 1164, 1893, 2049
EcoT38I	GRGCYC	7 cut(s)	657, 700, 1107, 1632, 1719, 1841, 1945
ErhI	CCWWGG	6 cut(s)	210, 355, 1000, 1164, 1893, 2049
Esp3I	CGTCTC	4 cut(s)	777, 1084, 1495, 1902
FaqI	GGGAC	4 cut(s)	754, 808, 1174, 1252
FauI	CCCGC	3 cut(s)	780, 1229, 2328
FblI	GTMKAC	2 cut(s)	981, 2220
FokI	GGATG	2 cut(s)	1435, 1812
FriOI	GRGCYC	7 cut(s)	657, 700, 1107, 1632, 1719, 1841, 1945
FspBI	CTAG	2 cut(s)	146, 1110
GlaI	GCGC	7 cut(s)	635, 776, 891, 893, 1048, 1403, 1977
GsaI	CCCAGC	2 cut(s)	135, 1413
GsuI	CTGGAG	3 cut(s)	55, 790, 937
HaeII	RGCGCY	1 cut(s)	1979
HgaI	GACGC	5 cut(s)	883, 967, 1384, 1919, 2437
HhaI	GCGC	7 cut(s)	636, 777, 892, 894, 1049, 1404, 1978
Hin1I	GRCGYC	4 cut(s)	546, 978, 984, 1395
Hin6I	GCGC	7 cut(s)	634, 775, 890, 892, 1047, 1402, 1976
HinP1I	GCGC	7 cut(s)	634, 775, 890, 892, 1047, 1402, 1976
HincII	GTYRAC	2 cut(s)	973, 982
HindII	GTYRAC	2 cut(s)	973, 982
HindIII	AAGCTT	1 cut(s)	139
HphI	GGTGA	8 cut(s)	1207, 1363, 1463, 1523, 1590, 1651, 1974, 2263
Hpy99I	CGWCG	9 cut(s)	557, 602, 862, 899, 983, 986, 1400, 1499, 1555
HpyAV	CCTTC	7 cut(s)	43, 50, 175, 458, 1966, 2142, 2253
HpyCH4III	ACNGT	9 cut(s)	62, 82, 439, 788, 794, 1100, 1150, 1472, 1579
HpyCH4IV	ACGT	5 cut(s)	20, 546, 984, 1378, 1665
HpyCH4V	TGCA	8 cut(s)	235, 678, 1487, 1805, 2024, 2096, 2342, 2423
HpyF3I	CTNAG	6 cut(s)	205, 319, 373, 1405, 1533, 2140
HpySE526I	ACGT	5 cut(s)	20, 546, 984, 1378, 1665
Hsp92I	GRCGYC	4 cut(s)	546, 978, 984, 1395
HspAI	GCGC	7 cut(s)	634, 775, 890, 892, 1047, 1402, 1976
KflI	GGGWCCC	1 cut(s)	822
Kpn2I	TCCGGA	2 cut(s)	379, 1828
KpnI	GGTACC	1 cut(s)	798
KspI	CCGCGG	1 cut(s)	816
Kzo9I	GATC	8 cut(s)	376, 618, 958, 1035, 1140, 1540, 1650, 2213
LguI	GCTCTTC	1 cut(s)	1739
Lsp1109I	GCAGC	3 cut(s)	78, 381, 1817
LweI	GCATC	2 cut(s)	337, 1377
MaeI	CTAG	2 cut(s)	146, 1110
MaeII	ACGT	5 cut(s)	20, 546, 984, 1378, 1665
MalI	GATC	8 cut(s)	378, 620, 960, 1037, 1142, 1542, 1652, 2215
MauBI	CGCGCGCG	1 cut(s)	890
MbiI	CCGCTC	1 cut(s)	1016
MboI	GATC	8 cut(s)	376, 618, 958, 1035, 1140, 1540, 1650, 2213
MboII	GAAGA	6 cut(s)	89, 95, 179, 182, 443, 1756
MflI	RGATCY	4 cut(s)	376, 618, 1650, 2213
MlsI	TGGCCA	2 cut(s)	833, 1124
MluCI	AATT	3 cut(s)	1633, 2189, 2363
MluNI	TGGCCA	2 cut(s)	833, 1124
MlyI	GAGTC	7 cut(s)	311, 404, 593, 776, 1515, 2235, 2325
MmeI	TCCRAC	7 cut(s)	339, 432, 535, 1132, 1311, 1574, 2340
Mox20I	TGGCCA	2 cut(s)	833, 1124
MroI	TCCGGA	2 cut(s)	379, 1828
MroXI	GAANNNNTTC	1 cut(s)	2104
MscI	TGGCCA	2 cut(s)	833, 1124
MseI	TTAA	2 cut(s)	8, 1815
MslI	CAYNNNNRTG	4 cut(s)	608, 1058, 2029, 2324
Msp20I	TGGCCA	2 cut(s)	833, 1124
MspA1I	CMGCKG	4 cut(s)	815, 1236, 1409, 1449
MspCI	CTTAAG	1 cut(s)	1814
Mva1269I	GAATGC	1 cut(s)	615
MvaI	CCWGG	4 cut(s)	521, 533, 1138, 1677
NcoI	CCATGG	2 cut(s)	210, 355
NdeII	GATC	8 cut(s)	376, 618, 958, 1035, 1140, 1540, 1650, 2213
NmeAIII	GCCGAG	2 cut(s)	184, 415
NmuCI	GTSAC	7 cut(s)	509, 788, 990, 1511, 1596, 1980, 2317
NruI	TCGCGA	1 cut(s)	1040
NspI	RCATGY	1 cut(s)	2060
NspV	TTCGAA	1 cut(s)	1931
OliI	CACNNNNGTG	1 cut(s)	2324
PagI	TCATGA	1 cut(s)	1143
PauI	GCGCGC	1 cut(s)	890
PceI	AGGCCT	2 cut(s)	164, 536
PciSI	GCTCTTC	1 cut(s)	1739
PcsI	WCGNNNNNNNCGW	5 cut(s)	426, 561, 975, 981, 1380
PctI	GAATGC	1 cut(s)	615
PdmI	GAANNNNTTC	1 cut(s)	2104
PfeI	GAWTC	4 cut(s)	260, 323, 491, 647
PflFI	GACNNNGTC	2 cut(s)	413, 977
PinAI	ACCGGT	2 cut(s)	1258, 1600
Ple19I	CGATCG	1 cut(s)	1038
PleI	GAGTC	7 cut(s)	311, 404, 592, 775, 1514, 2234, 2324
PmaCI	CACGTG	1 cut(s)	1379
PmlI	CACGTG	1 cut(s)	1379
PpsI	GAGTC	7 cut(s)	311, 404, 592, 775, 1514, 2234, 2324
Ppu21I	YACGTR	1 cut(s)	1379
PpuMI	RGGWCCY	2 cut(s)	822, 1161
PshAI	GACNNNNGTC	1 cut(s)	2198
Psp124BI	GAGCTC	1 cut(s)	700
Psp5II	RGGWCCY	2 cut(s)	822, 1161
Psp6I	CCWGG	4 cut(s)	519, 531, 1136, 1675
PspCI	CACGTG	1 cut(s)	1379
PspEI	GGTNACC	1 cut(s)	1511
PspFI	CCCAGC	2 cut(s)	131, 1409
PspGI	CCWGG	4 cut(s)	519, 531, 1136, 1675
PspOMI	GGGCCC	3 cut(s)	653, 1103, 1628
PspPPI	RGGWCCY	2 cut(s)	822, 1161
PstI	CTGCAG	1 cut(s)	1489
PsuI	RGATCY	4 cut(s)	376, 618, 1650, 2213
PsyI	GACNNNGTC	2 cut(s)	413, 977
PteI	GCGCGC	1 cut(s)	890
PvuI	CGATCG	1 cut(s)	1038
PvuII	CAGCTG	1 cut(s)	1409
RruI	TCGCGA	1 cut(s)	1040
RsaI	GTAC	8 cut(s)	23, 84, 287, 796, 1590, 1771, 2045, 2312
RsaNI	GTAC	8 cut(s)	22, 83, 286, 795, 1589, 1770, 2044, 2311
RseI	CAYNNNNRTG	4 cut(s)	608, 1058, 2029, 2324
SacI	GAGCTC	1 cut(s)	700
SacII	CCGCGG	1 cut(s)	816
SalI	GTCGAC	1 cut(s)	980
SapI	GCTCTTC	1 cut(s)	1739
SaqAI	TTAA	2 cut(s)	8, 1815
Sau3AI	GATC	8 cut(s)	376, 618, 958, 1035, 1140, 1540, 1650, 2213
SchI	GAGTC	7 cut(s)	311, 404, 593, 776, 1515, 2235, 2325
SfaNI	GCATC	2 cut(s)	337, 1377
SfcI	CTRYAG	1 cut(s)	1485
SfiI	GGCCNNNNNGGCC	1 cut(s)	161
Sfr303I	CCGCGG	1 cut(s)	816
SfuI	TTCGAA	1 cut(s)	1931
SgrAI	CRCCGGYG	1 cut(s)	1217
SgrBI	CCGCGG	1 cut(s)	816
SgrDI	CGTCGACG	1 cut(s)	980
SmaI	CCCGGG	2 cut(s)	2115, 2186
SmiMI	CAYNNNNRTG	4 cut(s)	608, 1058, 2029, 2324
SmlI	CTYRAG	4 cut(s)	136, 745, 1727, 1814
SmoI	CTYRAG	4 cut(s)	136, 745, 1727, 1814
Sse9I	AATT	3 cut(s)	1633, 2189, 2363
SseBI	AGGCCT	2 cut(s)	164, 536
SspI	AATATT	1 cut(s)	1687
SspMI	CTAG	2 cut(s)	146, 1110
SstI	GAGCTC	1 cut(s)	700
StuI	AGGCCT	2 cut(s)	164, 536
StyI	CCWWGG	6 cut(s)	210, 355, 1000, 1164, 1893, 2049
TaaI	ACNGT	9 cut(s)	62, 82, 439, 788, 794, 1100, 1150, 1472, 1579
TaiI	ACGT	5 cut(s)	23, 549, 987, 1381, 1668
TaqII	GACCGA	2 cut(s)	467, 1377
TasI	AATT	3 cut(s)	1633, 2189, 2363
TatI	WGTACW	3 cut(s)	82, 2043, 2310
TfiI	GAWTC	4 cut(s)	260, 323, 491, 647
Tru1I	TTAA	2 cut(s)	8, 1815
Tru9I	TTAA	2 cut(s)	8, 1815
TscAI	CASTG	2 cut(s)	647, 1105
TseFI	GTSAC	7 cut(s)	509, 788, 990, 1511, 1596, 1980, 2317
TseI	GCWGC	3 cut(s)	66, 394, 1805
Tsp45I	GTSAC	7 cut(s)	509, 788, 990, 1511, 1596, 1980, 2317
TspDTI	ATGAA	2 cut(s)	89, 254
TspGWI	ACGGA	1 cut(s)	403
TspMI	CCCGGG	2 cut(s)	2113, 2184
TspRI	CASTG	2 cut(s)	647, 1105
Tth111I	GACNNNGTC	2 cut(s)	413, 977
Vha464I	CTTAAG	1 cut(s)	1814
VneI	GTGCAC	1 cut(s)	2094
XapI	RAATTY	1 cut(s)	2363
XceI	RCATGY	1 cut(s)	2060
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2245
XmaI	CCCGGG	2 cut(s)	2113, 2184
XmiI	GTMKAC	2 cut(s)	981, 2220
XmnI	GAANNNNTTC	1 cut(s)	2104
XspI	CTAG	2 cut(s)	146, 1110
ZraI	GACGTC	2 cut(s)	547, 985

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.