Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0007347

Physical Location & Seq

Genomic Coordinates Reverse (-)

7503759 .. 7506514

Gene Structure 2756 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1094700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1149 bp

ATGGCCCCTAATTCGGAGTTCACAGACGGTACCCCAGCGCCCAACTCCCTCCAGGCCATTTGCTACAAGCGTGGCTCCCTTCAGCTACTCGATCAGACGAAGCTCCCATTGCAAAGTACGTATTTGGATATCCGGGACTCCACAGATGGATGGCACGCAATCAAAGATATGGTGGTGCGCGGCGCACCCGCCATTGCTATCGCGGCGGCTCTCTCATTGGCTGTGGAAATTTTTAACTTGGAGGATTTCAATGGAACCCCCGACGAAGCGTTCTCTTTCATTGTCATGAAATTGGAGTACCTTGTCTCCAGCCGACCAACTGCTGTCAATCTTTCGGATGCTGCAGCTAAACTGAAGCAAATTGCATCCGAAGTTGCCACTACTACTTCGGACGCAAGCAAAGTGTTTGAGGCTTACATAGAAGCTTCCGAAATCATGCTCAAGGACGATGTAGCCTCAAACAAGGCTATTGGATCTCATGGAGCAAATTTTATTCAACACCTGCTGGGAAACTCTAAGAACATTTCTATGTTGACCCATTGCAACACTGGCAGTCTAGCAACAGCTGGTTATGGTACTGCTCTTGGTGTCATCCGTTCACTTCATACCGAAGGAGTGTTAGAAAGGGCCTATTGTACAGAGACACGTCCATTCAACCAAGGATCCAGACTCACAGCATTTGAGTTGGTGCATGATAACATACCGGGCACTCTTATAGCAGATTCTGCTGCAGCTGCATTAATGAAATTTGGACGTGTGAATGCTGTAGTTGTTGGAGCAGATCGTGTGGCTACAAATGGTGACACCGCTAACAAGATTGGAACCTACAGTCTTGCCGTAAGTGCAAAGCACCATAACATTCCATTTTTCGTGGCTGCACCACTCACTTCCATCGACTTATCTCTTTCCTCCGGGGAAGAGATAATAATAGAGGAGAGATCCCCAAAGGAATTGTTGAACACACACGGAGGGCTCGGGGAACAAGTTGCTGCATCTGGAATTGCTGTCTGGAACCCAGCTTTCGATGTTACACCTGCCAATTTAATAACCGGTATCATAACCGAGAAGGGTGTTATTACCAAAAAGGGCACTGATGCTTTCAACATCAAGGGTTTTGTTCAGAAGGCAGCCGTGCAGTCCGGTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0000302 GO:0003674 GO:0003824 GO:0005575 GO:0005911 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009506 GO:0009719 GO:0009723 GO:0009725 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0019509 GO:0019752 GO:0030054 GO:0032870 GO:0033554 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0043094 GO:0043102 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0046394 GO:0046523 GO:0050896 GO:0051716 GO:0055044 GO:0070887 GO:0071241 GO:0071248 GO:0071265 GO:0071267 GO:0071281 GO:0071310 GO:0071369 GO:0071495 GO:0071704 GO:0071731 GO:0071732 GO:0097366 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 map00270 map01100

Pfam Domains

Protein Families

IF-2B

Protein Analysis

383

Amino Acids

40.32

Weight (kDa)

5.5

Isoelectric Point (pI)

35.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IF-2B	PF01008	59 - 360	6.9e-78	Initiation factor 2 subunit family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007347)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05830 AT2G05830 AT2G05830 AT2G05830
fragaria_vesca	FvH4_1g08940
malus_domestica	MD02G1094700.v1.1 MD15G1219600.v1.1
prunus_persica	Prupe.7G196300_v2.0.a1 Prupe.7G196300_v2.0.a1
pyrus_communis	pycom02g07490 pycom15g19470
rosa_chinensis	RchiOBHm_Chr2g0095391
rosa_laevigata	RLG00000016561
rosa_multiflora	Rmu_ssc0000183.1_g000040 Rmu_ssc0000183.1_g000041 Rmu_ssc0000183.1_g000042
rosa_roxburghii	Rroxscaffold_2G00146410
rosa_rugosa	Rorug02G0050400
rosa_samantha	Rh2AG098600 Rh2BG099800 Rh2CG101800 Rh2CG105700 Rh2DG099400
rosa_wichuraiana	Rw2G007440 Rw2G007730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	510, 1042
Acc36I	ACCTGC	2 cut(s)	510, 1042
Acc65I	GGTACC	1 cut(s)	29
AccB1I	GGYRCC	1 cut(s)	29
AccII	CGCG	2 cut(s)	180, 203
AciI	CCGC	5 cut(s)	180, 189, 203, 206, 807
AclWI	GGATC	4 cut(s)	481, 657, 670, 933
AcsI	RAATTY	3 cut(s)	228, 487, 746
AcuI	CTGAAG	2 cut(s)	65, 374
AfaI	GTAC	5 cut(s)	31, 118, 299, 577, 637
AfiI	CCNNNNNNNGG	1 cut(s)	13
AflIII	ACRYGT	2 cut(s)	644, 754
AgeI	ACCGGT	1 cut(s)	1049
AgsI	TTSAA	5 cut(s)	250, 497, 655, 958, 1102
AjiI	CACGTC	2 cut(s)	647, 755
AjnI	CCWGG	1 cut(s)	51
AluBI	AGCT	7 cut(s)	85, 103, 347, 425, 566, 734, 1019
AluI	AGCT	7 cut(s)	85, 103, 347, 425, 566, 734, 1019
Alw26I	GTCTC	2 cut(s)	310, 635
AlwI	GGATC	4 cut(s)	481, 657, 670, 933
AlwNI	CAGNNNCTG	1 cut(s)	725
Ama87I	CYCGRG	1 cut(s)	974
AoxI	GGCC	3 cut(s)	3, 54, 627
ApeKI	GCWGC	8 cut(s)	341, 344, 728, 731, 734, 875, 989, 1127
ApoI	RAATTY	3 cut(s)	228, 487, 746
AseI	ATTAAT	1 cut(s)	740
AsiGI	ACCGGT	1 cut(s)	1049
Asp718I	GGTACC	1 cut(s)	29
AspLEI	GCGC	3 cut(s)	40, 180, 185
AspS9I	GGNCC	2 cut(s)	4, 627
AsuC2I	CCSGG	3 cut(s)	134, 705, 913
AsuHPI	GGTGA	1 cut(s)	812
AvaI	CYCGRG	1 cut(s)	974
BaeGI	GKGCMC	2 cut(s)	710, 1091
BamHI	GGATCC	1 cut(s)	662
BanI	GGYRCC	1 cut(s)	29
BanII	GRGCYC	1 cut(s)	975
BbvI	GCAGC	8 cut(s)	328, 356, 715, 721, 743, 862, 976, 1139
BccI	CCATC	3 cut(s)	140, 144, 899
BceAI	ACGGC	2 cut(s)	821, 1115
BciT130I	CCWGG	1 cut(s)	53
BcnI	CCSGG	3 cut(s)	134, 705, 913
BcoDI	GTCTC	2 cut(s)	310, 635
BfaI	CTAG	1 cut(s)	557
BfmI	CTRYAG	4 cut(s)	342, 729, 765, 826
BfoI	RGCGCY	1 cut(s)	41
BfuAI	ACCTGC	2 cut(s)	510, 1042
Bme1390I	CCNGG	4 cut(s)	53, 134, 705, 913
BmeT110I	CYCGRG	1 cut(s)	974
BmgBI	CACGTC	2 cut(s)	647, 755
BmgT120I	GGNCC	2 cut(s)	4, 627
BmiI	GGNNCC	7 cut(s)	6, 31, 76, 256, 664, 823, 1013
BmrFI	CCNGG	4 cut(s)	53, 134, 705, 913
BmsI	GCATC	4 cut(s)	328, 374, 1001, 1084
BpmI	CTGGAG	2 cut(s)	35, 292
BpuEI	CTTGAG	1 cut(s)	425
BpuMI	CCSGG	3 cut(s)	134, 705, 913
BsaAI	YACGTR	1 cut(s)	120
BsaJI	CCNNGG	2 cut(s)	658, 912
BsaWI	WCCGGW	2 cut(s)	1049, 1139
BsaXI	ACNNNNNCTCC	2 cut(s)	290, 320
Bsc4I	CCNNNNNNNGG	1 cut(s)	13
Bse118I	RCCGGY	1 cut(s)	1049
Bse1I	ACTGG	1 cut(s)	553
Bse3DI	GCAATG	3 cut(s)	107, 192, 538
BseBI	CCWGG	1 cut(s)	53
BseDI	CCNNGG	2 cut(s)	658, 912
BseGI	GGATG	4 cut(s)	155, 343, 365, 591
BseLI	CCNNNNNNNGG	1 cut(s)	13
BseMI	GCAATG	3 cut(s)	107, 192, 538
BseNI	ACTGG	1 cut(s)	553
BseRI	GAGGAG	1 cut(s)	947
BseSI	GKGCMC	2 cut(s)	710, 1091
BseXI	GCAGC	8 cut(s)	328, 356, 715, 721, 743, 862, 976, 1139
BseYI	CCCAGC	3 cut(s)	34, 505, 1015
BsgI	GTGCAG	1 cut(s)	861
Bsh1236I	CGCG	2 cut(s)	180, 203
BshFI	GGCC	3 cut(s)	5, 56, 629
BshNI	GGYRCC	1 cut(s)	29
BshTI	ACCGGT	1 cut(s)	1049
BsiHKCI	CYCGRG	1 cut(s)	974
BsiSI	CCGG	5 cut(s)	133, 704, 912, 1050, 1140
BslFI	GGGAC	1 cut(s)	149
BslI	CCNNNNNNNGG	1 cut(s)	13
BsmAI	GTCTC	2 cut(s)	310, 635
BsmFI	GGGAC	1 cut(s)	149
BsmI	GAATGC	1 cut(s)	766
BsnI	GGCC	3 cut(s)	5, 56, 629
BsoBI	CYCGRG	1 cut(s)	974
Bsp1286I	GDGCHC	3 cut(s)	710, 975, 1091
Bsp1407I	TGTACA	1 cut(s)	635
Bsp143I	GATC	5 cut(s)	91, 473, 662, 781, 938
BspACI	CCGC	5 cut(s)	180, 189, 203, 206, 807
BspANI	GGCC	3 cut(s)	5, 56, 629
BspFNI	CGCG	2 cut(s)	180, 203
BspHI	TCATGA	1 cut(s)	285
BspLI	GGNNCC	7 cut(s)	6, 31, 76, 256, 664, 823, 1013
BspMAI	CTGCAG	2 cut(s)	346, 733
BspMI	ACCTGC	2 cut(s)	510, 1042
BspPI	GGATC	4 cut(s)	481, 657, 670, 933
BspT107I	GGYRCC	1 cut(s)	29
BsrDI	GCAATG	3 cut(s)	107, 192, 538
BsrFI	RCCGGY	1 cut(s)	1049
BsrGI	TGTACA	1 cut(s)	635
BsrI	ACTGG	1 cut(s)	553
BssAI	RCCGGY	1 cut(s)	1049
BssECI	CCNNGG	2 cut(s)	658, 912
BssMI	GATC	5 cut(s)	91, 473, 662, 781, 938
BssT1I	CCWWGG	1 cut(s)	658
Bst2UI	CCWGG	1 cut(s)	53
Bst4CI	ACNGT	2 cut(s)	29, 830
Bst6I	CTCTTC	1 cut(s)	912
BstAPI	GCANNNNNTGC	1 cut(s)	725
BstAUI	TGTACA	1 cut(s)	635
BstBAI	YACGTR	1 cut(s)	120
BstC8I	GCNNGC	2 cut(s)	156, 397
BstDEI	CTNAG	1 cut(s)	516
BstF5I	GGATG	4 cut(s)	155, 343, 365, 591
BstFNI	CGCG	2 cut(s)	180, 203
BstH2I	RGCGCY	1 cut(s)	41
BstHHI	GCGC	3 cut(s)	40, 180, 185
BstKTI	GATC	5 cut(s)	94, 476, 665, 784, 941
BstMAI	GTCTC	2 cut(s)	310, 635
BstMBI	GATC	5 cut(s)	91, 473, 662, 781, 938
BstMWI	GCNNNNNNNGC	6 cut(s)	109, 203, 549, 725, 734, 842
BstNI	CCWGG	1 cut(s)	53
BstSCI	CCNGG	4 cut(s)	51, 132, 703, 911
BstSFI	CTRYAG	4 cut(s)	342, 729, 765, 826
BstSLI	GKGCMC	2 cut(s)	710, 1091
BstSNI	TACGTA	1 cut(s)	120
BstUI	CGCG	2 cut(s)	180, 203
BstV1I	GCAGC	8 cut(s)	328, 356, 715, 721, 743, 862, 976, 1139
BstX2I	RGATCY	3 cut(s)	473, 662, 938
BstYI	RGATCY	3 cut(s)	473, 662, 938
BsuRI	GGCC	3 cut(s)	5, 56, 629
BtrI	CACGTC	2 cut(s)	647, 755
BtsCI	GGATG	4 cut(s)	155, 343, 365, 591
BtsIMutI	CAGTG	2 cut(s)	546, 1089
BveI	ACCTGC	2 cut(s)	510, 1042
Cac8I	GCNNGC	2 cut(s)	156, 397
CaiI	CAGNNNCTG	1 cut(s)	725
CciI	TCATGA	1 cut(s)	285
CfoI	GCGC	3 cut(s)	40, 180, 185
Cfr10I	RCCGGY	1 cut(s)	1049
Cfr13I	GGNCC	2 cut(s)	4, 627
CseI	GACGC	1 cut(s)	401
Csp6I	GTAC	5 cut(s)	30, 117, 298, 576, 636
CspAI	ACCGGT	1 cut(s)	1049
CviAII	CATG	4 cut(s)	286, 436, 479, 692
CviQI	GTAC	5 cut(s)	30, 117, 298, 576, 636
DdeI	CTNAG	1 cut(s)	516
DpnI	GATC	5 cut(s)	93, 475, 664, 783, 940
DpnII	GATC	5 cut(s)	91, 473, 662, 781, 938
Eam1104I	CTCTTC	1 cut(s)	912
EarI	CTCTTC	1 cut(s)	912
Eco105I	TACGTA	1 cut(s)	120
Eco130I	CCWWGG	1 cut(s)	658
Eco24I	GRGCYC	1 cut(s)	975
Eco32I	GATATC	1 cut(s)	130
Eco57I	CTGAAG	2 cut(s)	65, 374
Eco88I	CYCGRG	1 cut(s)	974
EcoO109I	RGGNCCY	1 cut(s)	627
EcoRII	CCWGG	1 cut(s)	51
EcoRV	GATATC	1 cut(s)	130
EcoT14I	CCWWGG	1 cut(s)	658
EcoT38I	GRGCYC	1 cut(s)	975
ErhI	CCWWGG	1 cut(s)	658
FaeI	CATG	4 cut(s)	289, 439, 482, 695
FaqI	GGGAC	1 cut(s)	149
FatI	CATG	4 cut(s)	285, 435, 478, 691
FauI	CCCGC	1 cut(s)	196
FokI	GGATG	4 cut(s)	162, 350, 352, 578
FriOI	GRGCYC	1 cut(s)	975
FspBI	CTAG	1 cut(s)	557
GlaI	GCGC	3 cut(s)	39, 179, 184
GsaI	CCCAGC	3 cut(s)	38, 509, 1019
GsuI	CTGGAG	2 cut(s)	35, 292
HaeII	RGCGCY	1 cut(s)	41
HaeIII	GGCC	3 cut(s)	5, 56, 629
HapII	CCGG	5 cut(s)	133, 704, 912, 1050, 1140
HgaI	GACGC	1 cut(s)	401
HhaI	GCGC	3 cut(s)	40, 180, 185
Hin1II	CATG	4 cut(s)	289, 439, 482, 695
Hin6I	GCGC	3 cut(s)	38, 178, 183
HinP1I	GCGC	3 cut(s)	38, 178, 183
HincII	GTYRAC	1 cut(s)	534
HindII	GTYRAC	1 cut(s)	534
HindIII	AAGCTT	1 cut(s)	423
HinfI	GANTC	3 cut(s)	137, 669, 722
HpaII	CCGG	5 cut(s)	133, 704, 912, 1050, 1140
HphI	GGTGA	1 cut(s)	812
Hpy166II	GTNNAC	3 cut(s)	21, 534, 599
Hpy188I	TCNGA	7 cut(s)	16, 96, 337, 370, 391, 430, 1122
Hpy188III	TCNNGA	4 cut(s)	286, 666, 996, 1009
Hpy8I	GTNNAC	3 cut(s)	21, 534, 599
Hpy99I	CGWCG	1 cut(s)	266
HpyAV	CCTTC	4 cut(s)	89, 605, 1060, 1117
HpyCH4III	ACNGT	2 cut(s)	29, 830
HpyCH4IV	ACGT	3 cut(s)	119, 646, 754
HpyF10VI	GCNNNNNNNGC	6 cut(s)	109, 203, 549, 725, 734, 842
HpyF3I	CTNAG	1 cut(s)	516
HpySE526I	ACGT	3 cut(s)	119, 646, 754
Hsp92II	CATG	4 cut(s)	289, 439, 482, 695
HspAI	GCGC	3 cut(s)	38, 178, 183
KpnI	GGTACC	1 cut(s)	33
Kzo9I	GATC	5 cut(s)	91, 473, 662, 781, 938
LmnI	GCTCC	4 cut(s)	80, 108, 482, 776
Lsp1109I	GCAGC	8 cut(s)	328, 356, 715, 721, 743, 862, 976, 1139
LweI	GCATC	4 cut(s)	328, 374, 1001, 1084
MaeI	CTAG	1 cut(s)	557
MaeII	ACGT	3 cut(s)	119, 646, 754
MaeIII	GTNAC	2 cut(s)	800, 1027
MalI	GATC	5 cut(s)	93, 475, 664, 783, 940
MboI	GATC	5 cut(s)	91, 473, 662, 781, 938
MboII	GAAGA	1 cut(s)	929
MflI	RGATCY	3 cut(s)	473, 662, 938
MhlI	GDGCHC	3 cut(s)	710, 975, 1091
MluCI	AATT	9 cut(s)	10, 228, 290, 360, 487, 746, 950, 999, 1039
MlyI	GAGTC	2 cut(s)	131, 663
MmeI	TCCRAC	1 cut(s)	754
MnlI	CCTC	7 cut(s)	59, 235, 403, 466, 919, 925, 962
MseI	TTAA	3 cut(s)	234, 740, 1043
MslI	CAYNNNNRTG	2 cut(s)	284, 527
MspA1I	CMGCKG	2 cut(s)	566, 734
MspI	CCGG	5 cut(s)	133, 704, 912, 1050, 1140
MspR9I	CCNGG	4 cut(s)	53, 134, 705, 913
MteI	GCGCNGCGC	1 cut(s)	181
Mva1269I	GAATGC	1 cut(s)	766
MvaI	CCWGG	1 cut(s)	53
MvnI	CGCG	2 cut(s)	180, 203
MwoI	GCNNNNNNNGC	6 cut(s)	109, 203, 549, 725, 734, 842
NciI	CCSGG	3 cut(s)	134, 705, 913
NdeII	GATC	5 cut(s)	91, 473, 662, 781, 938
NlaIII	CATG	4 cut(s)	289, 439, 482, 695
NlaIV	GGNNCC	7 cut(s)	6, 31, 76, 256, 664, 823, 1013
NmuCI	GTSAC	1 cut(s)	800
PagI	TCATGA	1 cut(s)	285
PaqCI	CACCTGC	2 cut(s)	510, 1042
PctI	GAATGC	1 cut(s)	766
PfeI	GAWTC	1 cut(s)	722
PfoI	TCCNGGA	1 cut(s)	132
PinAI	ACCGGT	1 cut(s)	1049
PleI	GAGTC	2 cut(s)	131, 663
PpsI	GAGTC	2 cut(s)	131, 663
Ppu21I	YACGTR	1 cut(s)	120
PshBI	ATTAAT	1 cut(s)	740
Psp6I	CCWGG	1 cut(s)	51
PspFI	CCCAGC	3 cut(s)	34, 505, 1015
PspGI	CCWGG	1 cut(s)	51
PspN4I	GGNNCC	7 cut(s)	6, 31, 76, 256, 664, 823, 1013
PspPI	GGNCC	2 cut(s)	4, 627
PstI	CTGCAG	2 cut(s)	346, 733
PstNI	CAGNNNCTG	1 cut(s)	725
PsuI	RGATCY	3 cut(s)	473, 662, 938
PvuII	CAGCTG	2 cut(s)	566, 734
RsaI	GTAC	5 cut(s)	31, 118, 299, 577, 637
RsaNI	GTAC	5 cut(s)	30, 117, 298, 576, 636
RseI	CAYNNNNRTG	2 cut(s)	284, 527
SaqAI	TTAA	3 cut(s)	234, 740, 1043
Sau3AI	GATC	5 cut(s)	91, 473, 662, 781, 938
Sau96I	GGNCC	2 cut(s)	4, 627
SchI	GAGTC	2 cut(s)	131, 663
ScrFI	CCNGG	4 cut(s)	53, 134, 705, 913
SduI	GDGCHC	3 cut(s)	710, 975, 1091
SfaNI	GCATC	4 cut(s)	328, 374, 1001, 1084
SfcI	CTRYAG	4 cut(s)	342, 729, 765, 826
SmiMI	CAYNNNNRTG	2 cut(s)	284, 527
SmlI	CTYRAG	1 cut(s)	440
SmoI	CTYRAG	1 cut(s)	440
SnaBI	TACGTA	1 cut(s)	120
Sse9I	AATT	9 cut(s)	10, 228, 290, 360, 487, 746, 950, 999, 1039
SsiI	CCGC	5 cut(s)	180, 189, 203, 206, 807
SspMI	CTAG	1 cut(s)	557
StyD4I	CCNGG	4 cut(s)	51, 132, 703, 911
StyI	CCWWGG	1 cut(s)	658
TaaI	ACNGT	2 cut(s)	29, 830
TaiI	ACGT	3 cut(s)	122, 649, 757
TaqI	TCGA	3 cut(s)	90, 894, 1023
TasI	AATT	9 cut(s)	10, 228, 290, 360, 487, 746, 950, 999, 1039
TatI	WGTACW	1 cut(s)	635
TauI	GCSGC	3 cut(s)	183, 206, 209
TfiI	GAWTC	1 cut(s)	722
Tru1I	TTAA	3 cut(s)	234, 740, 1043
Tru9I	TTAA	3 cut(s)	234, 740, 1043
TscAI	CASTG	2 cut(s)	553, 1096
TseFI	GTSAC	1 cut(s)	800
TseI	GCWGC	8 cut(s)	341, 344, 728, 731, 734, 875, 989, 1127
Tsp45I	GTSAC	1 cut(s)	800
TspDTI	ATGAA	4 cut(s)	268, 302, 593, 758
TspGWI	ACGGA	2 cut(s)	584, 981
TspRI	CASTG	2 cut(s)	553, 1096
VspI	ATTAAT	1 cut(s)	740
XapI	RAATTY	3 cut(s)	228, 487, 746
XcmI	CCANNNNNNNNNTGG	1 cut(s)	545
XspI	CTAG	1 cut(s)	557

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.