Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008380

Physical Location & Seq

Genomic Coordinates Reverse (-)

7632542 .. 7639481

Gene Structure 6940 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1096700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3456 bp

ATGAAGGTTCCCACCAATGGGTTATTAGCAAATTCTGGGGAAGCCGGAGAACAAAAGAGAATTAATTCGGAGTTATGGCATGCTTGTGCTGGGCCATTGGTTTCTTTGCCTCCGGTTGGAAGTCTTGTGGTGTACTTCCCACAAGGCCACAGCGAGCAAGTTGCAGCATCGATGCAAAAGGAGACTGATTTAATACCGAACTACACCAATCTTCCGTCTAAATTGATATGCATGCTTCACAATGTCACATTGCATGCTGATACCGAAACAGATGAGGTCTACGCACAAATGACTCTCCAACCAGTAAACAAATATGAGAAGGAAGCATTGCTGGCATCTGACATGGGCCTCAAACAAAGTAGGCAACCTGCTGAGTTTTTCTGCAAAACTCTTACAGCTAGCGACACGAGCACTCATGGTGGATTTTCTGTACCTCGTAGAGCAGCTGAGAAGATCCTCCCACCTCTAGATTTTTCTATGCAACCACCTGCTCAGGAGCTTGTTGCAAAAGATTTGCATGATAGTGCGTGGACATTCAGACATATCTATCGAGGCCAACCAAAACGGCACCTGCTGACTACGGGTTGGAGTGTTTTTGTTAGCAACAAAAGACTCTTTGCTGGAGATTCTGTTCTTTTTATAAGGGATGAAAAATCTCAGCTTCTCTTGGGTATAAGGCGTGCTCATAGACAGCAGCCAGCTCTCTCTTCATCAGTCATTTCCAGTGATAGCATGCATATCGGCATTCTTGCGGCTGCAGCTCATGCTGCTGCAAATAACAGTCCATTTACCATATTTTACAACCCAAGGGCAAGCCCTTCTGAATTTGTAGTACCTCTAGCCAAGTACAATAAAGCGATGTATACCCAAGTTTCACTTGGCATGCGATTCAGAATGATGTTTGAGACTGAGGAGTCAGGAGTACGCAGATACATGGGCACAGTCACTGGCATCAGCGATTTGGATCCTGTTAGATGGAAAGGTTCCCAGTGGCGCAATCTTCAGGTTGGATGGGATGAATCAACAGCTGGTGACCGGCCTAGCCGAGTTTCAATTTGGGAAATTGAGCCTGTTATAACTCCTTTCTACATATGCCCTCCTCCATTTTTCAGACCCAAGTTTCCCAAGCAACCAGGGATGCCAGATGATGAATCCGACATCGATAATGCTTTCAGGAGAGCCATGCCTTGGCTTGGAGATGAATTTGGCATGAAGAACTCGCCAAGCTCGTTTTTCCCTGGTTTGAGTTTGGTGCAGTGGATGAACATGCAACAAAATAATCAGTTTTCAGCTGCTCAGTCTGGATTTTTCCCATCCATGGTCCCTCCAACCGGCCTGCAAAATAACCTTGGTACTGATGATCCATCCAAGTTATTGAGTTTTCAAGCCCCGGTGCTGTCTTCGCTCGGTCTCCAGTCAAATAAATCAGCTCCACAAAACCATGCTAGTCAAATGCAGCAGCCGAATGTGACATGGGCTCAACAGCAGCAGCTGCAGCAGTTGATGCATAATCCTATGAACCAACAACAGCAAAGTAACCCCCAACAGCAGCAGCTGCAGCAATTATTGAATTCTTCGGTGAACCAACATCAGCAAAATCACCCCCCGCAGCAGCAGCTGCAGCAATTACTGAATTCTTCGGCAAACCAACATCAGCAAAATCACCCCCAGCAGCAGCAGCAATTATTGCAGACTCCAACGAACCAACAGCTCCATAATTTCTCCCATCAGCAGCAGCAGCGGGAGCCCCAACAACAGCAAGAGCTGCAAAACTTGTTGCAGCATCAGCAGCTGCATCAACAGCAACCACAACGGCACCAGCAACAACAATTATCTCAACCGAATCCGGTAAATAATGGTTCTGTTGCTCCTAACCAGGTCCCGGGCCAAAATTCACAACAACCAGTTATGTTCTCCCAACATCAACAGCAGCAATTACTAACAGGGAATACCCAGCAAGCTGTCCCTTCTTCTAGTAAGAATTCATTTCAGTTGCCAACTGGACAACAAAATTCACAGCTCCAGCAGCAGCAACTAGAACCGCAACCAAGCCTCTTACAAAGGCAGCAGCAGGCAGCACAATTGCAATCACCCCCACATCAATTGTTGCAACAGAGTGTGTCACAGAAAGTACAGCAGCAGCCACAAGTACAGCAATCATCTCCGCAGGGCATCTCTGAGCAACAACTCCAATTACAGTTGCTGCAGAAGTTTCAGCAACAGCAGCAGCCACAATTACTCTCCTCGTCAAGCTCACTTTTGCAGCCTCAACTCTTACAGCAGCAGCTGGCCCGCCAACAAAACCAGCAGTTACAACAGTTGCCTATGACTCAGCATCATCAGCAACAGCTAAGTGGCAACGGCTTCTCGACGGACAAACTTCTCAACAACTTTGCAGCTCCTTCGATGATGCAGTCACAACATATGCCTTCCATCCAGCCCCAGAACCAGCACAAGCCACTTACAGCAATCAGAAGCCATTCCGGTCTTACAGAAGGCGATGGTCCATCATGTTCAACTTCCCCTTCTACTAACTGCCAGATGTCCCCATCAAACTTCTTGAACAGGAATCAGCAAGGAATGGCCATGTTGTTGGGGGATTCAGTGGCCGAACCGGCTCATAATTTGGTTCAGGAGCTTCAGAGCAAGTCTGATGTTTGGGTCAAACATGAGCCGCCCAGCTCAAAAGGACCAGACCATATAAAATATAAAGGTACAATCACTGATCAGTTGGAAGCTTCATCATCTGGAACATCGTATTGTCTGGATACTAGCACAATCCATCAGAACTATGCACTTCCCACCTTCTGTTTGGATGGTGATGTTCAATCGAATCCTCGAAGCAGTCTTCCATTTTCCGCTAATATTGATGGATTGGCACCGGACACTTTGTTGTCAAGGGAATATGACTCTCAAAAAGATCTTCAGAACTTACTGTCTAATTATGGTGGGACGCCAAGAGACATTGAGACAGAGTTATCCACTGCTGCAATCAGCTCTCAGTCATTTGGGGCACCAAACATACCTTTCAAAGCTGGGTGCTCAAGTGATATTCCCATAACTGATGCTGGAGTTTTAGGCAACGGATCGTGGGCAAACCAGGCTCAACGTATGAGAACATATACAAAGGTTCAAAAGCGTGGATCTGTGGGAAGATGCATTGATGTCAACCGTTATAAAGGCTATGATGAGCTCCGGCAAGCTCTTGCCCGCATGTTTGGGATTGAAGGGCAACTAGAAGAGCCACACAGAACGGACTGGAAACTTGTATACGTGGATCATGAAAATGAGATTCTTCTCGTTGGTGATGATCCATGGGAGGAGTTTGTGAGCTGTGTCCAGAGTATAAAGATATTGTCATCCGTTGAAGTACAGCAGATGAGCTTGGATGGAGACCTCGGCAACATTCCAGTCCCCAATCAAGCTTCTAGCGGAACTGCCAGTGGAAATGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009791 GO:0009889 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010311 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022622 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0046983 GO:0048364 GO:0048366 GO:0048367 GO:0048527 GO:0048528 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:1905392 GO:1905393 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

1152

Amino Acids

128.34

Weight (kDa)

6.4

Isoelectric Point (pI)

71.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	127 - 228	1.3e-20	B3 DNA binding domain
BRWD_AD	PF25313	255 - 358	3.7e-06	BRWD/PHIP ancillary domain-like domain
ARF_AD	PF06507	276 - 358	8.3e-36	Auxin response factor ancillary domain
AUX_IAA	PF02309	1034 - 1125	2.8e-09	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008380)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19220 AT5G20730 AT5G20730 AT5G20730
fragaria_vesca	FvH4_1g09150 FvH4_1g09150 FvH4_1g09150
malus_domestica	MD02G1096700.v1.1 MD15G1221400.v1.1
prunus_persica	Prupe.7G194200_v2.0.a1 Prupe.7G194200_v2.0.a1
pyrus_communis	pycom02g07670
rosa_chinensis	RchiOBHm_Chr2g0095551
rosa_laevigata	RLG00000016578
rosa_multiflora	Rmu_sc0005382.1_g000007 Rmu_sc0038699.1_g000002
rosa_roxburghii	Rroxscaffold_2G00146200
rosa_rugosa	Rorug02G0052300
rosa_samantha	Rh2AG100100 Rh2BG101600 Rh2CG103700 Rh2DG101000
rosa_wichuraiana	Rw2G007600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	641, 1076, 3177
AarI	CACCTGC	2 cut(s)	496, 579
AasI	GACNNNNNNGTC	1 cut(s)	913
Acc36I	ACCTGC	3 cut(s)	376, 496, 579
AccB1I	GGYRCC	4 cut(s)	567, 1815, 2878, 3013
AccB7I	CCANNNNNTGG	1 cut(s)	1188
AccI	GTMKAC	3 cut(s)	279, 863, 3270
AclWI	GGATC	8 cut(s)	448, 959, 972, 1355, 3094, 3151, 3285, 3305
AcoI	YGGCCR	2 cut(s)	2583, 2607
AcsI	RAATTY	8 cut(s)	31, 824, 1202, 1570, 1633, 1891, 1981, 2011
AcuI	CTGAAG	3 cut(s)	986, 2624, 2909
AcyI	GRCGYC	1 cut(s)	2954
AfiI	CCNNNNNNNGG	8 cut(s)	17, 18, 116, 1188, 1193, 1318, 1331, 1882
AjnI	CCWGG	4 cut(s)	1132, 1237, 1875, 3099
AloI	GAACNNNNNNTCC	2 cut(s)	615, 647
Alw21I	GWGCWC	4 cut(s)	413, 685, 3044, 3195
Alw26I	GTCTC	6 cut(s)	176, 899, 1415, 2955, 2963, 3387
AlwI	GGATC	8 cut(s)	448, 959, 972, 1355, 3094, 3151, 3285, 3305
AlwNI	CAGNNNCTG	7 cut(s)	947, 1492, 1555, 1618, 1792, 2203, 2287
Ama87I	CYCGRG	1 cut(s)	1882
ApoI	RAATTY	8 cut(s)	31, 824, 1202, 1570, 1633, 1891, 1981, 2011
AseI	ATTAAT	1 cut(s)	63
Asp700I	GAANNNNTTC	2 cut(s)	64, 2479
AspLEI	GCGC	1 cut(s)	996
AspS9I	GGNCC	8 cut(s)	92, 346, 1321, 1879, 1885, 2290, 2504, 2690
AsuC2I	CCSGG	3 cut(s)	1391, 1883, 1884
AsuHPI	GGTGA	7 cut(s)	1043, 1591, 1592, 1655, 2082, 2831, 3317
AsuNHI	GCTAGC	1 cut(s)	398
AvaI	CYCGRG	1 cut(s)	1882
AvaII	GGWCC	4 cut(s)	1321, 1879, 2504, 2690
BaeGI	GKGCMC	2 cut(s)	941, 3016
BalI	TGGCCA	1 cut(s)	2585
BamHI	GGATCC	1 cut(s)	964
BanI	GGYRCC	4 cut(s)	567, 1815, 2878, 3013
BanII	GRGCYC	3 cut(s)	1480, 1749, 3195
BauI	CACGAG	1 cut(s)	406
BbsI	GAAGAC	2 cut(s)	1392, 2840
Bbv12I	GWGCWC	4 cut(s)	413, 685, 3044, 3195
BceAI	ACGGC	3 cut(s)	581, 1829, 2377
BcgI	CGANNNNNNTGC	6 cut(s)	143, 177, 721, 755, 2385, 2419
BciT130I	CCWGG	4 cut(s)	1134, 1239, 1877, 3101
BciVI	GTATCC	1 cut(s)	2761
BclI	TGATCA	1 cut(s)	2725
BcnI	CCSGG	3 cut(s)	1391, 1883, 1884
BcoDI	GTCTC	6 cut(s)	176, 899, 1415, 2955, 2963, 3387
BfmI	CTRYAG	5 cut(s)	756, 1493, 1556, 1619, 2204
BfuAI	ACCTGC	3 cut(s)	376, 496, 579
BfuI	GTATCC	1 cut(s)	2761
BglI	GCCNNNNNGGC	1 cut(s)	2615
BglII	AGATCT	1 cut(s)	2920
Bme1390I	CCNGG	7 cut(s)	1134, 1239, 1391, 1877, 1883, 1884, 3101
Bme18I	GGWCC	4 cut(s)	1321, 1879, 2504, 2690
BmeT110I	CYCGRG	1 cut(s)	1882
BmgT120I	GGNCC	8 cut(s)	92, 346, 1321, 1879, 1885, 2290, 2504, 2690
BmrFI	CCNGG	7 cut(s)	1134, 1239, 1391, 1877, 1883, 1884, 3101
BmrI	ACTGGG	1 cut(s)	982
BmtI	GCTAGC	1 cut(s)	402
BmuI	ACTGGG	1 cut(s)	982
BpiI	GAAGAC	2 cut(s)	1392, 2840
BpmI	CTGGAG	4 cut(s)	642, 1397, 2006, 3090
Bpu10I	CCTNAGC	1 cut(s)	492
BpuEI	CTTGAG	1 cut(s)	3028
BpuMI	CCSGG	3 cut(s)	1391, 1883, 1884
Bsa29I	ATCGAT	2 cut(s)	170, 1161
BsaAI	YACGTR	1 cut(s)	3274
BsaBI	GATNNNNATC	1 cut(s)	963
BsaHI	GRCGYC	1 cut(s)	2954
BsaI	GGTCTC	2 cut(s)	1415, 3387
BsaWI	WCCGGW	4 cut(s)	112, 1846, 2483, 2881
BsaXI	ACNNNNNCTCC	2 cut(s)	615, 645
Bsc4I	CCNNNNNNNGG	8 cut(s)	17, 18, 116, 1188, 1193, 1318, 1331, 1882
Bse118I	RCCGGY	3 cut(s)	1035, 1331, 2614
Bse3DI	GCAATG	2 cut(s)	248, 326
Bse8I	GATNNNNATC	1 cut(s)	963
BseBI	CCWGG	4 cut(s)	1134, 1239, 1877, 3101
BseCI	ATCGAT	2 cut(s)	170, 1161
BseJI	GATNNNNATC	1 cut(s)	963
BseLI	CCNNNNNNNGG	8 cut(s)	17, 18, 116, 1188, 1193, 1318, 1331, 1882
BseMI	GCAATG	2 cut(s)	248, 326
BseMII	CTCAG	9 cut(s)	363, 438, 506, 671, 900, 1310, 2169, 2345, 3014
BseRI	GAGGAG	4 cut(s)	926, 1089, 2233, 3335
BseSI	GKGCMC	2 cut(s)	941, 3016
BseYI	CCCAGC	5 cut(s)	89, 1668, 1953, 2678, 3035
BsgI	GTGCAG	1 cut(s)	1274
BshNI	GGYRCC	4 cut(s)	567, 1815, 2878, 3013
BshVI	ATCGAT	2 cut(s)	170, 1161
BsiHKAI	GWGCWC	4 cut(s)	413, 685, 3044, 3195
BsiHKCI	CYCGRG	1 cut(s)	1882
BslFI	GGGAC	6 cut(s)	1307, 1865, 1949, 2530, 2965, 3398
BslI	CCNNNNNNNGG	8 cut(s)	17, 18, 116, 1188, 1193, 1318, 1331, 1882
BsmAI	GTCTC	6 cut(s)	176, 899, 1415, 2955, 2963, 3387
BsmFI	GGGAC	6 cut(s)	1307, 1865, 1949, 2530, 2965, 3398
BsmI	GAATGC	1 cut(s)	744
Bso31I	GGTCTC	2 cut(s)	1415, 3387
BsoBI	CYCGRG	1 cut(s)	1882
Bsp1286I	GDGCHC	8 cut(s)	413, 685, 941, 1480, 1749, 3016, 3044, 3195
Bsp143I	GATC	9 cut(s)	453, 964, 1360, 2725, 2920, 3086, 3143, 3277, 3310
Bsp19I	CCATGG	2 cut(s)	1317, 3314
BspCNI	CTCAG	9 cut(s)	364, 439, 505, 670, 901, 1309, 2170, 2344, 3013
BspDI	ATCGAT	2 cut(s)	170, 1161
BspHI	TCATGA	1 cut(s)	3280
BspMAI	CTGCAG	5 cut(s)	760, 1497, 1560, 1623, 2208
BspMI	ACCTGC	3 cut(s)	376, 496, 579
BspOI	GCTAGC	1 cut(s)	402
BspPI	GGATC	8 cut(s)	448, 959, 972, 1355, 3094, 3151, 3285, 3305
BspQI	GCTCTTC	1 cut(s)	3234
BspT107I	GGYRCC	4 cut(s)	567, 1815, 2878, 3013
BspTNI	GGTCTC	2 cut(s)	1415, 3387
BsrDI	GCAATG	2 cut(s)	248, 326
BsrFI	RCCGGY	3 cut(s)	1035, 1331, 2614
BssAI	RCCGGY	3 cut(s)	1035, 1331, 2614
BssMI	GATC	9 cut(s)	453, 964, 1360, 2725, 2920, 3086, 3143, 3277, 3310
BssNAI	GTATAC	2 cut(s)	864, 3271
BssNI	GRCGYC	1 cut(s)	2954
BssSI	CACGAG	1 cut(s)	406
BssT1I	CCWWGG	5 cut(s)	806, 1187, 1317, 1348, 3314
Bst1107I	GTATAC	2 cut(s)	864, 3271
Bst2BI	CACGAG	1 cut(s)	406
Bst2UI	CCWGG	4 cut(s)	1134, 1239, 1877, 3101
Bst4CI	ACNGT	6 cut(s)	782, 943, 2199, 2319, 2937, 3173
Bst6I	CTCTTC	2 cut(s)	712, 3234
BstACI	GRCGYC	1 cut(s)	2954
BstAPI	GCANNNNNTGC	7 cut(s)	764, 1492, 1504, 1555, 1618, 1687, 1765
BstBAI	YACGTR	1 cut(s)	3274
BstDSI	CCRYGG	2 cut(s)	1317, 3314
BstEII	GGTNACC	1 cut(s)	1031
BstHHI	GCGC	1 cut(s)	996
BstKTI	GATC	9 cut(s)	456, 967, 1363, 2728, 2923, 3089, 3146, 3280, 3313
BstMAI	GTCTC	6 cut(s)	176, 899, 1415, 2955, 2963, 3387
BstMBI	GATC	9 cut(s)	453, 964, 1360, 2725, 2920, 3086, 3143, 3277, 3310
BstNI	CCWGG	4 cut(s)	1134, 1239, 1877, 3101
BstNSI	RCATGY	7 cut(s)	83, 235, 257, 736, 886, 1270, 3217
BstPI	GGTNACC	1 cut(s)	1031
BstSCI	CCNGG	7 cut(s)	1132, 1237, 1389, 1875, 1881, 1882, 3099
BstSFI	CTRYAG	5 cut(s)	756, 1493, 1556, 1619, 2204
BstSLI	GKGCMC	2 cut(s)	941, 3016
BstV2I	GAAGAC	2 cut(s)	1392, 2840
BstX2I	RGATCY	4 cut(s)	453, 964, 2920, 3143
BstXI	CCANNNNNNTGG	1 cut(s)	2593
BstYI	RGATCY	4 cut(s)	453, 964, 2920, 3143
BstZ17I	GTATAC	2 cut(s)	864, 3271
Bsu15I	ATCGAT	2 cut(s)	170, 1161
BsuI	GTATCC	1 cut(s)	2761
BsuTUI	ATCGAT	2 cut(s)	170, 1161
BtgI	CCRYGG	2 cut(s)	1317, 3314
BtgZI	GCGATG	2 cut(s)	872, 2514
BtsI	GCAGTG	2 cut(s)	1262, 2982
BtsIMutI	CAGTG	8 cut(s)	730, 945, 995, 1262, 2610, 2721, 2982, 3448
BveI	ACCTGC	3 cut(s)	376, 496, 579
CaiI	CAGNNNCTG	7 cut(s)	947, 1492, 1555, 1618, 1792, 2203, 2287
CciI	TCATGA	1 cut(s)	3280
CfoI	GCGC	1 cut(s)	996
Cfr10I	RCCGGY	3 cut(s)	1035, 1331, 2614
Cfr13I	GGNCC	8 cut(s)	92, 346, 1321, 1879, 1885, 2290, 2504, 2690
Cfr9I	CCCGGG	1 cut(s)	1882
ClaI	ATCGAT	2 cut(s)	170, 1161
CseI	GACGC	1 cut(s)	2962
CsiI	ACCWGGT	1 cut(s)	1875
CspCI	CAANNNNNGTGG	2 cut(s)	2085, 2120
DpnI	GATC	9 cut(s)	455, 966, 1362, 2727, 2922, 3088, 3145, 3279, 3312
DpnII	GATC	9 cut(s)	453, 964, 1360, 2725, 2920, 3086, 3143, 3277, 3310
DrdI	GACNNNNNNGTC	1 cut(s)	913
DseDI	GACNNNNNNGTC	1 cut(s)	913
EaeI	YGGCCR	2 cut(s)	2583, 2607
Eam1104I	CTCTTC	2 cut(s)	712, 3234
EarI	CTCTTC	2 cut(s)	712, 3234
Ecl136II	GAGCTC	1 cut(s)	3193
Eco130I	CCWWGG	5 cut(s)	806, 1187, 1317, 1348, 3314
Eco24I	GRGCYC	3 cut(s)	1480, 1749, 3195
Eco31I	GGTCTC	2 cut(s)	1415, 3387
Eco47I	GGWCC	4 cut(s)	1321, 1879, 2504, 2690
Eco53kI	GAGCTC	1 cut(s)	3193
Eco57I	CTGAAG	3 cut(s)	986, 2624, 2909
Eco88I	CYCGRG	1 cut(s)	1882
Eco91I	GGTNACC	1 cut(s)	1031
EcoICRI	GAGCTC	1 cut(s)	3193
EcoO109I	RGGNCCY	1 cut(s)	1879
EcoO65I	GGTNACC	1 cut(s)	1031
EcoRI	GAATTC	3 cut(s)	1570, 1633, 1981
EcoRII	CCWGG	4 cut(s)	1132, 1237, 1875, 3099
EcoT14I	CCWWGG	5 cut(s)	806, 1187, 1317, 1348, 3314
EcoT22I	ATGCAT	5 cut(s)	233, 738, 1509, 3161, 3454
EcoT38I	GRGCYC	3 cut(s)	1480, 1749, 3195
ErhI	CCWWGG	5 cut(s)	806, 1187, 1317, 1348, 3314
FalI	AAGNNNNNCTT	2 cut(s)	861, 893
FaqI	GGGAC	6 cut(s)	1307, 1865, 1949, 2530, 2965, 3398
FauI	CCCGC	4 cut(s)	1614, 1734, 2300, 3218
FauNDI	CATATG	2 cut(s)	1091, 2424
FbaI	TGATCA	1 cut(s)	2725
FblI	GTMKAC	3 cut(s)	279, 863, 3270
FriOI	GRGCYC	3 cut(s)	1480, 1749, 3195
GlaI	GCGC	1 cut(s)	995
GsaI	CCCAGC	5 cut(s)	93, 1672, 1957, 2682, 3039
GsuI	CTGGAG	4 cut(s)	642, 1397, 2006, 3090
HgaI	GACGC	1 cut(s)	2962
HhaI	GCGC	1 cut(s)	996
Hin1I	GRCGYC	1 cut(s)	2954
Hin6I	GCGC	1 cut(s)	994
HinP1I	GCGC	1 cut(s)	994
HincII	GTYRAC	1 cut(s)	3169
HindII	GTYRAC	1 cut(s)	3169
HindIII	AAGCTT	2 cut(s)	2736, 3423
HphI	GGTGA	7 cut(s)	1043, 1591, 1592, 1655, 2082, 2831, 3317
Hpy166II	GTNNAC	8 cut(s)	133, 280, 307, 531, 864, 1582, 3169, 3271
Hpy8I	GTNNAC	8 cut(s)	133, 280, 307, 531, 864, 1582, 3169, 3271
Hpy99I	CGWCG	1 cut(s)	2374
HpyAV	CCTTC	9 cut(s)	313, 828, 1977, 2412, 2439, 2489, 2535, 2815, 3221
HpyCH4III	ACNGT	6 cut(s)	782, 943, 2199, 2319, 2937, 3173
HpyCH4IV	ACGT	2 cut(s)	3109, 3273
HpySE526I	ACGT	2 cut(s)	3109, 3273
Hsp92I	GRCGYC	1 cut(s)	2954
HspAI	GCGC	1 cut(s)	994
Ksp22I	TGATCA	1 cut(s)	2725
Kzo9I	GATC	9 cut(s)	453, 964, 1360, 2725, 2920, 3086, 3143, 3277, 3310
LguI	GCTCTTC	1 cut(s)	3234
LmnI	GCTCC	9 cut(s)	496, 1435, 1716, 1744, 1873, 2025, 2404, 2635, 3198
MabI	ACCWGGT	1 cut(s)	1875
MaeII	ACGT	2 cut(s)	3109, 3273
MaeIII	GTNAC	8 cut(s)	244, 943, 1031, 1468, 1535, 2121, 2310, 2415
MalI	GATC	9 cut(s)	455, 966, 1362, 2727, 2922, 3088, 3145, 3279, 3312
MboI	GATC	9 cut(s)	453, 964, 1360, 2725, 2920, 3086, 3143, 3277, 3310
MfeI	CAATTG	2 cut(s)	2081, 2102
MflI	RGATCY	4 cut(s)	453, 964, 2920, 3143
MhlI	GDGCHC	8 cut(s)	413, 685, 941, 1480, 1749, 3016, 3044, 3195
MlsI	TGGCCA	1 cut(s)	2585
MluNI	TGGCCA	1 cut(s)	2585
MlyI	GAGTC	6 cut(s)	286, 606, 923, 1687, 2323, 2903
MmeI	TCCRAC	8 cut(s)	97, 322, 566, 988, 1179, 1352, 1721, 2712
Mox20I	TGGCCA	1 cut(s)	2585
Mph1103I	ATGCAT	5 cut(s)	233, 738, 1509, 3161, 3454
MroXI	GAANNNNTTC	2 cut(s)	64, 2479
MscI	TGGCCA	1 cut(s)	2585
MseI	TTAA	2 cut(s)	63, 191
MslI	CAYNNNNRTG	3 cut(s)	84, 522, 3285
Msp20I	TGGCCA	1 cut(s)	2585
MspA1I	CMGCKG	9 cut(s)	446, 1028, 1292, 1492, 1555, 1618, 1741, 1792, 2287
MspR9I	CCNGG	7 cut(s)	1134, 1239, 1391, 1877, 1883, 1884, 3101
MunI	CAATTG	2 cut(s)	2081, 2102
Mva1269I	GAATGC	1 cut(s)	744
MvaI	CCWGG	4 cut(s)	1134, 1239, 1877, 3101
NciI	CCSGG	3 cut(s)	1391, 1883, 1884
NcoI	CCATGG	2 cut(s)	1317, 3314
NdeI	CATATG	2 cut(s)	1091, 2424
NdeII	GATC	9 cut(s)	453, 964, 1360, 2725, 2920, 3086, 3143, 3277, 3310
NheI	GCTAGC	1 cut(s)	398
NmeAIII	GCCGAG	2 cut(s)	1070, 3378
NmuCI	GTSAC	6 cut(s)	244, 943, 1031, 1468, 2121, 2415
NsiI	ATGCAT	5 cut(s)	233, 738, 1509, 3161, 3454
NspI	RCATGY	7 cut(s)	83, 235, 257, 736, 886, 1270, 3217
PaeI	GCATGC	5 cut(s)	83, 235, 257, 736, 886
PagI	TCATGA	1 cut(s)	3280
PaqCI	CACCTGC	2 cut(s)	496, 579
PciSI	GCTCTTC	1 cut(s)	3234
PcsI	WCGNNNNNNNCGW	1 cut(s)	1226
PctI	GAATGC	1 cut(s)	744
PdmI	GAANNNNTTC	2 cut(s)	64, 2479
PfeI	GAWTC	9 cut(s)	626, 888, 1019, 1151, 1843, 2569, 2600, 2833, 3292
PflMI	CCANNNNNTGG	1 cut(s)	1188
PleI	GAGTC	6 cut(s)	286, 606, 922, 1687, 2323, 2903
PpsI	GAGTC	6 cut(s)	286, 606, 922, 1687, 2323, 2903
Ppu21I	YACGTR	1 cut(s)	3274
PpuMI	RGGWCCY	1 cut(s)	1879
PshBI	ATTAAT	1 cut(s)	63
PsiI	TTATAA	3 cut(s)	641, 1076, 3177
Psp124BI	GAGCTC	1 cut(s)	3195
Psp5II	RGGWCCY	1 cut(s)	1879
Psp6I	CCWGG	4 cut(s)	1132, 1237, 1875, 3099
PspEI	GGTNACC	1 cut(s)	1031
PspFI	CCCAGC	5 cut(s)	89, 1668, 1953, 2678, 3035
PspGI	CCWGG	4 cut(s)	1132, 1237, 1875, 3099
PspPI	GGNCC	8 cut(s)	92, 346, 1321, 1879, 1885, 2290, 2504, 2690
PspPPI	RGGWCCY	1 cut(s)	1879
PstI	CTGCAG	5 cut(s)	760, 1497, 1560, 1623, 2208
PstNI	CAGNNNCTG	7 cut(s)	947, 1492, 1555, 1618, 1792, 2203, 2287
PsuI	RGATCY	4 cut(s)	453, 964, 2920, 3143
PvuII	CAGCTG	8 cut(s)	446, 1028, 1292, 1492, 1555, 1618, 1792, 2287
RseI	CAYNNNNRTG	3 cut(s)	84, 522, 3285
SacI	GAGCTC	1 cut(s)	3195
SapI	GCTCTTC	1 cut(s)	3234
SaqAI	TTAA	2 cut(s)	63, 191
Sau3AI	GATC	9 cut(s)	453, 964, 1360, 2725, 2920, 3086, 3143, 3277, 3310
Sau96I	GGNCC	8 cut(s)	92, 346, 1321, 1879, 1885, 2290, 2504, 2690
SchI	GAGTC	6 cut(s)	286, 606, 923, 1687, 2323, 2903
ScrFI	CCNGG	7 cut(s)	1134, 1239, 1391, 1877, 1883, 1884, 3101
SduI	GDGCHC	8 cut(s)	413, 685, 941, 1480, 1749, 3016, 3044, 3195
SexAI	ACCWGGT	1 cut(s)	1875
SfcI	CTRYAG	5 cut(s)	756, 1493, 1556, 1619, 2204
SinI	GGWCC	4 cut(s)	1321, 1879, 2504, 2690
SmaI	CCCGGG	1 cut(s)	1884
SmiMI	CAYNNNNRTG	3 cut(s)	84, 522, 3285
SmlI	CTYRAG	1 cut(s)	3043
SmoI	CTYRAG	1 cut(s)	3043
SphI	GCATGC	5 cut(s)	83, 235, 257, 736, 886
SspI	AATATT	1 cut(s)	2866
SstI	GAGCTC	1 cut(s)	3195
StyD4I	CCNGG	7 cut(s)	1132, 1237, 1389, 1875, 1881, 1882, 3099
StyI	CCWWGG	5 cut(s)	806, 1187, 1317, 1348, 3314
TaaI	ACNGT	6 cut(s)	782, 943, 2199, 2319, 2937, 3173
TaiI	ACGT	2 cut(s)	3112, 3276
TaqI	TCGA	7 cut(s)	170, 550, 1161, 2369, 2405, 2831, 2839
TaqII	GACCGA	1 cut(s)	1397
TatI	WGTACW	5 cut(s)	132, 846, 2131, 2149, 3370
TauI	GCSGC	2 cut(s)	755, 2677
TfiI	GAWTC	9 cut(s)	626, 888, 1019, 1151, 1843, 2569, 2600, 2833, 3292
Tru1I	TTAA	2 cut(s)	63, 191
Tru9I	TTAA	2 cut(s)	63, 191
TscAI	CASTG	8 cut(s)	730, 952, 995, 1262, 2610, 2728, 2989, 3448
TseFI	GTSAC	6 cut(s)	244, 943, 1031, 1468, 2121, 2415
Tsp45I	GTSAC	6 cut(s)	244, 943, 1031, 1468, 2121, 2415
TspGWI	ACGGA	5 cut(s)	204, 2387, 3099, 3269, 3352
TspMI	CCCGGG	1 cut(s)	1882
TspRI	CASTG	8 cut(s)	730, 952, 995, 1262, 2610, 2728, 2989, 3448
Van91I	CCANNNNNTGG	1 cut(s)	1188
VpaK11BI	GGWCC	4 cut(s)	1321, 1879, 2504, 2690
VspI	ATTAAT	1 cut(s)	63
XapI	RAATTY	8 cut(s)	31, 824, 1202, 1570, 1633, 1891, 1981, 2011
XbaI	TCTAGA	1 cut(s)	466
XceI	RCATGY	7 cut(s)	83, 235, 257, 736, 886, 1270, 3217
XcmI	CCANNNNNNNNNTGG	2 cut(s)	875, 2809
XmaI	CCCGGG	1 cut(s)	1882
XmiI	GTMKAC	3 cut(s)	279, 863, 3270
XmnI	GAANNNNTTC	2 cut(s)	64, 2479
Zsp2I	ATGCAT	5 cut(s)	233, 738, 1509, 3161, 3454

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.