MD02G1099300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012977
Physical Location & Seq
Reverse (-)
7873920 .. 7874492
573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1099300.v1.1.491

Sequence Viewer

Length: 573 bp
ATGAACGTGTTGGATTCTAATCTCGAGGCTCTTGCTGTCAATTACTTGAGCTTCGGATTGTTCACGACAACTTGGGTCGCCGTTTTGACCGCCGCCATTAGCTTCTGGAGGCTCAGACCCAGAGCCGCCGCCGCCGCCACCAGCAGTGCCATCTCTTCTTCTTCATCTTCTTCATTGAGTTTCCGCTCACAACCTGCCGTTTCCGACCTCAGCTTGCATGGGTCGTCGCCGGTCTCCGAGATAACGCAGTCAGATGCGGCGGCGAAAAAGGTGGACCCGCCATGCTCTGTTCCATCATTGGCTAATTTCGATGACGGCAGAGTGCAGCTGACAAAGGGTTCGAAGTTTGCGGTGTACTTTGAGGATAGTAGCGTTGAGAGTGACGTTGACCTGGCCGCGACCTCATCGCTTATGGCGGAGGAGGATGAGACGGAATGGAGCGGCAGCGATGAGGTGGTGGTGGCTAATTGGTGGGAGGATGTGTTGAGGTTGAGAACGGGGGAGATGGGTTGGTACAGGTTCCAGGACTTGACGGAGCTAAACGGTAACGTCGTTAGATTATGGGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.66

Weight (kDa)

4.3

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012977)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43110 AT5G20790 AT5G20790
fragaria_vesca FvH4_1g09230
malus_domestica MD02G1099300.v1.1 MD15G1222300.v1.1
prunus_persica Prupe.7G193600_v2.0.a1
pyrus_communis pycom02g07740
rosa_chinensis RchiOBHm_Chr2g0095761
rosa_laevigata RLG00000016590
rosa_multiflora Rmu_sc0005941.1_g000003
rosa_roxburghii Rroxscaffold_2G00146120
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101400 Rh2BG103100 Rh2CG105400 Rh2DG102800
rosa_wichuraiana Rw2G007690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 202
AccBSI CCGCTC 2 cut(s) 186, 441
AccII CGCG 1 cut(s) 398
AcoI YGGCCR 1 cut(s) 393
AfaI GTAC 2 cut(s) 356, 515
AflIII ACRYGT 1 cut(s) 6
AjnI CCWGG 2 cut(s) 390, 522
AluBI AGCT 5 cut(s) 51, 102, 213, 328, 538
AluI AGCT 5 cut(s) 51, 102, 213, 328, 538
Alw26I GTCTC 2 cut(s) 238, 422
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 2 cut(s) 325, 444
ArsI GACNNNNNNTTYG 2 cut(s) 322, 354
AspS9I GGNCC 1 cut(s) 274
AsuII TTCGAA 1 cut(s) 341
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 1 cut(s) 274
BbvCI CCTCAGC 1 cut(s) 209
BbvI GCAGC 2 cut(s) 337, 456
BccI CCATC 3 cut(s) 158, 301, 499
BceAI ACGGC 3 cut(s) 65, 182, 331
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 2 cut(s) 392, 524
BcoDI GTCTC 2 cut(s) 238, 422
BfuAI ACCTGC 1 cut(s) 202
Bme1390I CCNGG 2 cut(s) 392, 524
Bme18I GGWCC 1 cut(s) 274
BmeT110I CYCGRG 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 2 cut(s) 276, 521
BmrFI CCNGG 2 cut(s) 392, 524
BmsI GCATC 1 cut(s) 244
BpmI CTGGAG 1 cut(s) 127
Bpu10I CCTNAGC 1 cut(s) 209
Bpu14I TTCGAA 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 238
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bse118I RCCGGY 1 cut(s) 229
Bse8I GATNNNNATC 1 cut(s) 18
BseBI CCWGG 2 cut(s) 392, 524
BseGI GGATG 3 cut(s) 430, 484, 571
BseJI GATNNNNATC 1 cut(s) 18
BseMII CTCAG 2 cut(s) 127, 223
BseRI GAGGAG 1 cut(s) 434
BseXI GCAGC 2 cut(s) 337, 456
BsgI GTGCAG 1 cut(s) 344
Bsh1236I CGCG 1 cut(s) 398
BshFI GGCC 1 cut(s) 395
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 1 cut(s) 230
BsmAI GTCTC 2 cut(s) 238, 422
BsmBI CGTCTC 1 cut(s) 422
BsnI GGCC 1 cut(s) 395
Bso31I GGTCTC 1 cut(s) 238
BsoBI CYCGRG 1 cut(s) 23
Bsp119I TTCGAA 1 cut(s) 341
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 2 cut(s) 126, 222
BspFNI CGCG 1 cut(s) 398
BspLI GGNNCC 2 cut(s) 276, 521
BspMI ACCTGC 1 cut(s) 202
BspT104I TTCGAA 1 cut(s) 341
BspTNI GGTCTC 1 cut(s) 238
BsrBI CCGCTC 2 cut(s) 186, 441
BsrFI RCCGGY 1 cut(s) 229
BssAI RCCGGY 1 cut(s) 229
Bst2UI CCWGG 2 cut(s) 392, 524
Bst4CI ACNGT 1 cut(s) 545
Bst6I CTCTTC 1 cut(s) 160
BstBI TTCGAA 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 2 cut(s) 113, 209
BstF5I GGATG 3 cut(s) 430, 484, 571
BstFNI CGCG 1 cut(s) 398
BstMAI GTCTC 2 cut(s) 238, 422
BstMWI GCNNNNNNNGC 2 cut(s) 131, 134
BstNI CCWGG 2 cut(s) 392, 524
BstSCI CCNGG 2 cut(s) 390, 522
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 2 cut(s) 337, 456
BsuRI GGCC 1 cut(s) 395
BtgZI GCGATG 2 cut(s) 390, 462
BtsCI GGATG 3 cut(s) 430, 484, 571
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 151
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 1 cut(s) 215
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 355, 514
CviAII CATG 2 cut(s) 218, 282
CviQI GTAC 2 cut(s) 355, 514
DdeI CTNAG 2 cut(s) 113, 209
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 160
EarI CTCTTC 1 cut(s) 160
EciI GGCGGA 1 cut(s) 431
Eco31I GGTCTC 1 cut(s) 238
Eco47I GGWCC 1 cut(s) 274
Eco88I CYCGRG 1 cut(s) 23
EcoRII CCWGG 2 cut(s) 390, 522
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 2 cut(s) 221, 285
FaiI YATR 4 cut(s) 219, 283, 413, 562
FatI CATG 2 cut(s) 217, 281
FauI CCCGC 1 cut(s) 285
FokI GGATG 2 cut(s) 437, 491
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 1 cut(s) 395
HapII CCGG 1 cut(s) 230
Hin1II CATG 2 cut(s) 221, 285
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 1 cut(s) 14
HpaII CCGG 1 cut(s) 230
Hpy166II GTNNAC 4 cut(s) 63, 274, 355, 388
Hpy188I TCNGA 5 cut(s) 56, 116, 205, 238, 253
Hpy188III TCNNGA 3 cut(s) 23, 64, 106
Hpy8I GTNNAC 4 cut(s) 63, 274, 355, 388
Hpy99I CGWCG 2 cut(s) 229, 554
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4IV ACGT 3 cut(s) 6, 384, 549
HpyCH4V TGCA 2 cut(s) 217, 325
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 134
HpyF3I CTNAG 2 cut(s) 113, 209
HpySE526I ACGT 3 cut(s) 6, 384, 549
Hsp92II CATG 2 cut(s) 221, 285
LmnI GCTCC 2 cut(s) 438, 535
Lsp1109I GCAGC 2 cut(s) 337, 456
LweI GCATC 1 cut(s) 244
MaeII ACGT 3 cut(s) 6, 384, 549
MaeIII GTNAC 2 cut(s) 380, 545
MbiI CCGCTC 2 cut(s) 186, 441
MboII GAAGA 5 cut(s) 147, 150, 153, 159, 162
MluCI AATT 3 cut(s) 40, 304, 466
MmeI TCCRAC 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 328
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 2 cut(s) 392, 524
MvaI CCWGG 2 cut(s) 392, 524
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 2 cut(s) 131, 134
NlaIII CATG 2 cut(s) 221, 285
NlaIV GGNNCC 2 cut(s) 276, 521
NmuCI GTSAC 1 cut(s) 380
NspV TTCGAA 1 cut(s) 341
PaeR7I CTCGAG 1 cut(s) 23
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PfeI GAWTC 1 cut(s) 14
PfoI TCCNGGA 1 cut(s) 522
Psp6I CCWGG 2 cut(s) 390, 522
PspGI CCWGG 2 cut(s) 390, 522
PspN4I GGNNCC 2 cut(s) 276, 521
PspPI GGNCC 1 cut(s) 274
PvuII CAGCTG 1 cut(s) 328
RsaI GTAC 2 cut(s) 356, 515
RsaNI GTAC 2 cut(s) 355, 514
Sau96I GGNCC 1 cut(s) 274
ScrFI CCNGG 2 cut(s) 392, 524
SfaNI GCATC 1 cut(s) 244
Sfr274I CTCGAG 1 cut(s) 23
SfuI TTCGAA 1 cut(s) 341
SinI GGWCC 1 cut(s) 274
SlaI CTCGAG 1 cut(s) 23
SmlI CTYRAG 2 cut(s) 23, 46
SmoI CTYRAG 2 cut(s) 23, 46
Sse9I AATT 3 cut(s) 40, 304, 466
StyD4I CCNGG 2 cut(s) 390, 522
TaaI ACNGT 1 cut(s) 545
TaiI ACGT 3 cut(s) 9, 387, 552
TaqI TCGA 3 cut(s) 24, 309, 341
TasI AATT 3 cut(s) 40, 304, 466
TatI WGTACW 1 cut(s) 354
TauI GCSGC 9 cut(s) 95, 128, 131, 134, 137, 260, 263, 398, 444
TfiI GAWTC 1 cut(s) 14
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 2 cut(s) 325, 444
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 3 cut(s) 17, 153, 162
TspGWI ACGGA 2 cut(s) 446, 548
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 274
XhoI CTCGAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.