Rorug02G0053000

21 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0012977
Physical Location & Seq
Forward (+)
4054697 .. 4063794
9098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0053000.1

Sequence Viewer

Length: 2694 bp
ATGCCTCTCAGGCTTGACATTAAGCGCAAGTTTGCGCAAAGATCCGAGAGAGTGAAGTCTTTGGATCTGCATCCAACTGAACCATGGATCCTTGCAAGTCTTTACTCAGGAACCGTGTATATCTTGAACTACCAGTCACAGACCATGGCAAAGTCATTTGAAGTCTCTGAATTGCCAGTTCGGTCAGCAAAATTCGTAACACGAAAGCAATGGGTAGTTGCTGCAGCCGATGACATGTGTATACGTGTTTACAATTACAATACCATGGATAAGGTTAAAGTCTTTGAAGCACACAGTGACTACATTAGATGTGTGGCTGTCCATCCAACACTTCCATATGTTTTGTCATCATCTGATGACATGCTTATCAAACTGTGGGATTGGGAAAAGGGGTGGATGTGTACTCAGATATTTGAAGGGCATTCTCACTATGTCATGCAAGTGACATTTAACCCCAAAGACACCAACACTTTTGCAAGTGCTTCCCTTGATCACACTGTAAAGATTTGGAATCTTGTCTCCCCTGACCCAAATTTTACATTGGATGCCCACCTGAAAGGGGTCAATTGTGTTGATTACTTCACTGGTGGTGATAAACCGTACTTAATCACGGGATCTGATGATCACACTGCAAAGGTATGGGACTATCAAACAAAAAGTTGTGTTCAGACACTTGAAGGACACACTCATAATGTATCTGCAGTATGCTTCCACCCTGAACTTCCAGTTATAATTACTGGTTCTGAGGATGGAACTGTTAGAATATGGCATTCAACCACTTACAGGCTTGAAAACACATTGAATTATGGGCTGGAACGTGTTTGGGCTTTTGGGTATATGAAAGGATCACGTCGGATTGTTATTGGTTATGACGAAGGAACCATTTTGGTAAAAATTGGTCGAGAGGTACCGGTTGCTAGCATGGATAGTAGTGGGAAAATAATCTGGGCCAAGCATAATGAAATCCAAACTGTGAATATCAAAAGTGTGGGATCAGATTTTGAGATTACAGATGGAGAAAGATTACCATTGGCCGTGAAGGAGTTGGGAACCTGTGATCTTTATCCTCAAAATTTGAAGCACAACCCCAATGGAAGGTTTGTTGTTGTTTGTGGTGATGGAGAGTACATAATATACACAGCTCTAGCATGGAGAAATAGATCCTTTGGCTCGGCATTGGAGTTTGTCTGGTCATCAGATGGAGAATATGCTGTTAGGGAAACAACATCAAAGATCAAAATTTTCAGCAAAACATTCCAGGAAAAGAAGAATATCCGACCAACATTTTCTGTTGAACACATTTATGGAGGGACACTATTGGCAATGTGTTCAAATGACTTTGTATGCTTCTATGACTGGGGGGAATGCAGATTGATTAGACGAATTGATGTCAATGTTAAGAATGTTTATTGGGCTGATAGCGGTGATTTGGTGGCAATTTCTAGTGATTCATCATTCTACATTCTAAAATACGATAGGGATGTAGTCTCTTCTTATTTTGACAGTGGAAGGCCAGTTGATGAACTAGGTGTTGAGGATGCTTTTGAGCTGCTTTTTGAGATAAATGAGCGTGTCCGAACTGGATTATGGGTTGGCGACTGTTTCGTTTATAATAACTCCTCCTGGCGACTCAATTACTGTGTTGGTGGTGAGGTGACCACAATGTTTCATCTGGACCGGCCCATGTACTTGTTAGGATATCTTGCAAATCAAAGTCGTCTTTTTCTTATCGATAAAGAGTTCAATGTCATGGGATACTCCTTGCTTCTCAGCTTGATTGAGTACAAAACACTTGTTATGCGTGGAGAATTAGAGCGTGCCAAACAAATATTTCCAACAATTCCCCAAGAGCAACATAACAGTGTGGCTCGGTTTTTGGAATCTCGAGGGATGTTGGAGGATGCACTGGAAGTGGCTACAGATCCTGACTACAAATTTGATCTTGCTATACAGCTGGGTAATCTGGAGATTGCAAAGGAAATTGCTACTAAAGCACAGAGTGAATCCAAATGGAAGCAGCTGGGAGATTTAGCTATGTCCTCTGGGAAGTTAGAACTTGCTGAAGATTGCCTATTATATGGAATGGATTTTTCTGGCTTGCTACTCCTGTATTCTTCTCTTGGAGATGCTGAAGGAATATCAAAACTTGCTTCAATTGCTAAAGAGCAGGGAAAGAATAATGTTGCATTCCTTTGTTTGTTCTTGCTGGGTAAATTGGAAGAATGCATCCAGCTGTTGCTAGATAGTGAGCGGATACCTGAAGCAGCTTTGATGGCACGATCTTACCTACCCAGCAAGGTGTCAGAGATAGTTTCAATTTGGAGAAATGACCTGAACAAGGTTAATAAAAGAGCTGCAGAATCTTTGGCAGATCCCCAAGAATATCCTAATTTGTTTGAGGACTGGCAGATTTCTCTTTCCCTCGAGTCTAAACGCACTGAAAATAGTGGCAATTATCAACCAGCTGAGCAGTACCCAGTCTATGCTTCTGAGAAGCCAGCCAGCAGTCTTGTTGAATTGTTCAAAAGCATGCACATTGATGAAGAAGAAGCCGCCCCAGTTGAAAATGGAGATCTGGATCATGAGGAGTTAGAGGAGAATGGAGAAGATCAAGGTGAAGTACATACCATTGAGGAGGACTCAGACTCCATGAATGGTGTTGTTCTTGTGAATGGCACCCAGGAGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0030234 GO:0043086 GO:0044092 GO:0048509 GO:0050789 GO:0050790 GO:0050793 GO:0065007 GO:0065009 GO:0098772 GO:1902183
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

897

Amino Acids

101.77

Weight (kDa)

4.93

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 10 - 155 1.6e-14 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 12 - 150 3.3e-11 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 13 - 131 6.9e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 14 - 153 2.7e-19 WDR5 beta-propeller domain
WD40_Prp19 PF24814 16 - 184 4.1e-20 Prp19 WD40 domain
WDR55 PF24796 16 - 198 2.9e-15 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 21 - 174 1.1e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 21 - 191 4.1e-23 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 35 - 193 5.1e-16 CDC20/Fizzy WD40 domain
Beta-prop_EIPR1 PF23609 53 - 170 6.2e-08 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 58 - 131 3.3e-11 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 59 - 195 6.6e-27 WDR5 beta-propeller domain
WD40_Gbeta PF25391 67 - 211 2.3e-12 G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd PF24782 68 - 172 1.4e-08 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 69 - 260 7.1e-13 TEP-1 second beta-propeller
Beta-prop_EML PF23409 76 - 265 4.3e-15 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 91 - 127 5.3e-08 WD domain, G-beta repeat
WD40 PF00400 133 - 171 1e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 135 - 295 2.5e-27 WDR3 first beta-propeller domain
Beta-prop_RIG_2nd PF23775 135 - 245 5.7e-06 RIG second beta-propeller
EIF3I PF24805 137 - 228 3.1e-06 EIF3I
WDR55 PF24796 139 - 296 6.7e-14 WDR55
Beta-prop_WDR3_2nd PF25172 140 - 260 5e-14 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 154 - 288 7e-29 WDR5 beta-propeller domain
WD40_Gbeta PF25391 154 - 259 2.4e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st PF25171 164 - 256 5.8e-06 WDR36/Utp21 first beta-propeller
WD40 PF00400 177 - 215 2.2e-06 WD domain, G-beta repeat
WD40_Prp19 PF24814 184 - 265 2.7e-15 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 186 - 295 2.6e-15 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 196 - 265 1.1e-06 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 198 - 270 1.6e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR75_1st PF23869 198 - 261 2.3e-06 WD repeat-containing protein 75 first beta-propeller
WD40_WDHD1_1st PF24817 198 - 322 3e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 199 - 296 4.1e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 202 - 258 2.7e-07 WDR90/POC16, second beta-propeller
Beta-prop_SCAP PF24017 202 - 259 4.4e-06 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 206 - 261 8e-07 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 220 - 256 2.5e-11 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 234 - 298 8.3e-06 Echinoderm microtubule-associated protein second beta-propeller
B-prop_COPA_B_2nd PF04053 319 - 578 4.6e-87 COPA/B second beta-propeller
TPR_COPA_B PF23953 595 - 775 2.5e-80 COPA/B TPR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012977)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43110 AT5G20790 AT5G20790
fragaria_vesca FvH4_1g09230
malus_domestica MD02G1099300.v1.1 MD15G1222300.v1.1
prunus_persica Prupe.7G193600_v2.0.a1
pyrus_communis pycom02g07740
rosa_chinensis RchiOBHm_Chr2g0095761
rosa_laevigata RLG00000016590
rosa_multiflora Rmu_sc0005941.1_g000003
rosa_roxburghii Rroxscaffold_2G00146120
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101400 Rh2BG103100 Rh2CG105400 Rh2DG102800
rosa_wichuraiana Rw2G007690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 731, 1611
Acc16I TGCGCA 1 cut(s) 36
Acc65I GGTACC 1 cut(s) 907
AccB1I GGYRCC 2 cut(s) 907, 2675
AccBSI CCGCTC 1 cut(s) 2251
AccI GTMKAC 1 cut(s) 241
AciI CCGC 3 cut(s) 1422, 2251, 2553
AcoI YGGCCR 1 cut(s) 1032
AcsI RAATTY 5 cut(s) 191, 532, 1072, 1239, 1934
AcuI CTGAAG 3 cut(s) 2082, 2151, 2280
AdeI CACNNNGTG 2 cut(s) 296, 2000
AfaI GTAC 8 cut(s) 403, 602, 909, 1127, 1688, 1784, 2474, 2622
AfiI CCNNNNNNNGG 4 cut(s) 559, 783, 1095, 2338
AflIII ACRYGT 3 cut(s) 234, 244, 817
AgeI ACCGGT 1 cut(s) 910
AjiI CACGTC 1 cut(s) 851
AjnI CCWGG 3 cut(s) 1257, 1622, 2679
AloI GAACNNNNNNTCC 2 cut(s) 72, 104
Alw26I GTCTC 3 cut(s) 169, 523, 1492
AlwNI CAGNNNCTG 1 cut(s) 1925
Ama87I CYCGRG 2 cut(s) 1884, 2423
AoxI GGCC 4 cut(s) 948, 1032, 1511, 1679
ApeKI GCWGC 6 cut(s) 221, 224, 1549, 2017, 2264, 2354
ApoI RAATTY 5 cut(s) 191, 532, 1072, 1239, 1934
ArsI GACNNNNNNTTYG 2 cut(s) 1919, 1951
AsiGI ACCGGT 1 cut(s) 910
Asp718I GGTACC 1 cut(s) 907
AspLEI GCGC 2 cut(s) 27, 37
AspS9I GGNCC 3 cut(s) 948, 1675, 1680
AsuHPI GGTGA 6 cut(s) 602, 1127, 1436, 1661, 1666, 2627
AsuNHI GCTAGC 1 cut(s) 917
AvaI CYCGRG 2 cut(s) 1884, 2423
AvaII GGWCC 1 cut(s) 1675
BamHI GGATCC 1 cut(s) 87
BanI GGYRCC 2 cut(s) 907, 2675
BbvI GCAGC 6 cut(s) 208, 236, 1536, 2029, 2276, 2341
BccI CCATC 6 cut(s) 330, 743, 1007, 1112, 1193, 2266
BceAI ACGGC 1 cut(s) 1019
BciT130I CCWGG 3 cut(s) 1259, 1624, 2681
BciVI GTATCC 2 cut(s) 1748, 2247
BclI TGATCA 2 cut(s) 490, 622
BcoDI GTCTC 3 cut(s) 169, 523, 1492
BfaI CTAG 5 cut(s) 918, 1145, 1443, 1526, 2240
BfmI CTRYAG 4 cut(s) 222, 699, 1917, 2355
BfuI GTATCC 2 cut(s) 1748, 2247
BglI GCCNNNNNGGC 1 cut(s) 10
BglII AGATCT 1 cut(s) 2572
BisI GCNGC 7 cut(s) 222, 225, 1550, 2018, 2265, 2355, 2553
BlpI GCTNAGC 1 cut(s) 2466
BlsI GCNGC 7 cut(s) 223, 226, 1551, 2019, 2266, 2356, 2554
Bme1390I CCNGG 3 cut(s) 1259, 1624, 2681
Bme18I GGWCC 1 cut(s) 1675
BmeT110I CYCGRG 2 cut(s) 1884, 2423
BmgBI CACGTC 1 cut(s) 851
BmgT120I GGNCC 3 cut(s) 948, 1675, 1680
BmiI GGNNCC 6 cut(s) 89, 112, 880, 909, 1051, 2677
BmrFI CCNGG 3 cut(s) 1259, 1624, 2681
BmrI ACTGGG 3 cut(s) 1366, 2471, 2552
BmsI GCATC 6 cut(s) 79, 535, 1528, 1891, 2116, 2235
BmtI GCTAGC 1 cut(s) 921
BmuI ACTGGG 3 cut(s) 1366, 2471, 2552
BplI GAGNNNNNCTC 2 cut(s) 2624, 2656
BpmI CTGGAG 1 cut(s) 1985
Bpu1102I GCTNAGC 1 cut(s) 2466
Bsa29I ATCGAT 1 cut(s) 1731
BsaAI YACGTR 1 cut(s) 245
BsaBI GATNNNNATC 3 cut(s) 69, 1062, 2577
BsaJI CCNNGG 4 cut(s) 83, 144, 264, 2679
BsaWI WCCGGW 1 cut(s) 910
BsaXI ACNNNNNCTCC 3 cut(s) 2630, 2660, 2675
Bsc4I CCNNNNNNNGG 4 cut(s) 559, 783, 1095, 2338
Bse118I RCCGGY 2 cut(s) 910, 1677
Bse3DI GCAATG 2 cut(s) 215, 1329
Bse8I GATNNNNATC 3 cut(s) 69, 1062, 2577
BseBI CCWGG 3 cut(s) 1259, 1624, 2681
BseCI ATCGAT 1 cut(s) 1731
BseDI CCNNGG 4 cut(s) 83, 144, 264, 2679
BseJI GATNNNNATC 3 cut(s) 69, 1062, 2577
BseLI CCNNNNNNNGG 4 cut(s) 559, 783, 1095, 2338
BseMI GCAATG 2 cut(s) 215, 1329
BseMII CTCAG 8 cut(s) 22, 120, 419, 735, 1783, 2457, 2481, 2655
BseRI GAGGAG 4 cut(s) 1609, 2600, 2609, 2648
BseXI GCAGC 6 cut(s) 208, 236, 1536, 2029, 2276, 2341
BseYI CCCAGC 4 cut(s) 1954, 2020, 2206, 2291
BshFI GGCC 4 cut(s) 950, 1034, 1513, 1681
BshNI GGYRCC 2 cut(s) 907, 2675
BshTI ACCGGT 1 cut(s) 910
BshVI ATCGAT 1 cut(s) 1731
BsiHKCI CYCGRG 2 cut(s) 1884, 2423
BsiSI CCGG 2 cut(s) 911, 1678
BslFI GGGAC 2 cut(s) 656, 1324
BslI CCNNNNNNNGG 4 cut(s) 559, 783, 1095, 2338
BsmAI GTCTC 3 cut(s) 169, 523, 1492
BsmFI GGGAC 2 cut(s) 656, 1324
BsmI GAATGC 5 cut(s) 421, 769, 1370, 2186, 2228
BsnI GGCC 4 cut(s) 950, 1034, 1513, 1681
BsoBI CYCGRG 2 cut(s) 1884, 2423
Bsp1720I GCTNAGC 1 cut(s) 2466
Bsp19I CCATGG 3 cut(s) 83, 144, 264
BspACI CCGC 3 cut(s) 1422, 2251, 2553
BspANI GGCC 4 cut(s) 950, 1034, 1513, 1681
BspCNI CTCAG 8 cut(s) 21, 119, 418, 736, 1782, 2458, 2482, 2654
BspDI ATCGAT 1 cut(s) 1731
BspHI TCATGA 1 cut(s) 2581
BspLI GGNNCC 6 cut(s) 89, 112, 880, 909, 1051, 2677
BspMAI CTGCAG 3 cut(s) 226, 703, 2359
BspOI GCTAGC 1 cut(s) 921
BspT107I GGYRCC 2 cut(s) 907, 2675
BsrBI CCGCTC 1 cut(s) 2251
BsrDI GCAATG 2 cut(s) 215, 1329
BsrFI RCCGGY 2 cut(s) 910, 1677
BssAI RCCGGY 2 cut(s) 910, 1677
BssECI CCNNGG 4 cut(s) 83, 144, 264, 2679
BssNAI GTATAC 1 cut(s) 242
BssT1I CCWWGG 3 cut(s) 83, 144, 264
Bst1107I GTATAC 1 cut(s) 242
Bst2UI CCWGG 3 cut(s) 1259, 1624, 2681
Bst6I CTCTTC 2 cut(s) 1495, 2682
BstBAI YACGTR 1 cut(s) 245
BstC8I GCNNGC 6 cut(s) 919, 1818, 2099, 2499, 2503, 2531
BstDEI CTNAG 8 cut(s) 8, 106, 405, 744, 1769, 2466, 2490, 2641
BstDSI CCRYGG 3 cut(s) 83, 144, 264
BstEII GGTNACC 1 cut(s) 1654
BstENI CCTNNNNNAGG 1 cut(s) 2336
BstHHI GCGC 2 cut(s) 27, 37
BstMAI GTCTC 3 cut(s) 169, 523, 1492
BstMWI GCNNNNNNNGC 4 cut(s) 10, 1991, 2156, 2273
BstNI CCWGG 3 cut(s) 1259, 1624, 2681
BstNSI RCATGY 3 cut(s) 238, 364, 2533
BstPI GGTNACC 1 cut(s) 1654
BstSCI CCNGG 3 cut(s) 1257, 1622, 2679
BstSFI CTRYAG 4 cut(s) 222, 699, 1917, 2355
BstV1I GCAGC 6 cut(s) 208, 236, 1536, 2029, 2276, 2341
BstX2I RGATCY 8 cut(s) 41, 64, 87, 614, 1160, 1921, 2371, 2572
BstYI RGATCY 8 cut(s) 41, 64, 87, 614, 1160, 1921, 2371, 2572
BstZ17I GTATAC 1 cut(s) 242
Bsu15I ATCGAT 1 cut(s) 1731
BsuI GTATCC 2 cut(s) 1748, 2247
BsuRI GGCC 4 cut(s) 950, 1034, 1513, 1681
BsuTUI ATCGAT 1 cut(s) 1731
BtgI CCRYGG 3 cut(s) 83, 144, 264
BtrI CACGTC 1 cut(s) 851
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 8 cut(s) 301, 495, 582, 627, 1510, 1867, 1904, 2436
Cac8I GCNNGC 6 cut(s) 919, 1818, 2099, 2499, 2503, 2531
CaiI CAGNNNCTG 1 cut(s) 1925
CciI TCATGA 1 cut(s) 2581
CfoI GCGC 2 cut(s) 27, 37
Cfr10I RCCGGY 2 cut(s) 910, 1677
Cfr13I GGNCC 3 cut(s) 948, 1675, 1680
ClaI ATCGAT 1 cut(s) 1731
Csp6I GTAC 8 cut(s) 402, 601, 908, 1126, 1687, 1783, 2473, 2621
CspAI ACCGGT 1 cut(s) 910
CviQI GTAC 8 cut(s) 402, 601, 908, 1126, 1687, 1783, 2473, 2621
DdeI CTNAG 8 cut(s) 8, 106, 405, 744, 1769, 2466, 2490, 2641
DraIII CACNNNGTG 2 cut(s) 296, 2000
EaeI YGGCCR 1 cut(s) 1032
Eam1104I CTCTTC 2 cut(s) 1495, 2682
EarI CTCTTC 2 cut(s) 1495, 2682
Eco130I CCWWGG 3 cut(s) 83, 144, 264
Eco32I GATATC 1 cut(s) 1700
Eco47I GGWCC 1 cut(s) 1675
Eco57I CTGAAG 3 cut(s) 2082, 2151, 2280
Eco88I CYCGRG 2 cut(s) 1884, 2423
Eco91I GGTNACC 1 cut(s) 1654
EcoNI CCTNNNNNAGG 1 cut(s) 2336
EcoO65I GGTNACC 1 cut(s) 1654
EcoRII CCWGG 3 cut(s) 1257, 1622, 2679
EcoRV GATATC 1 cut(s) 1700
EcoT14I CCWWGG 3 cut(s) 83, 144, 264
EcoT22I ATGCAT 1 cut(s) 2228
ErhI CCWWGG 3 cut(s) 83, 144, 264
FaqI GGGAC 2 cut(s) 656, 1324
FauNDI CATATG 1 cut(s) 337
FbaI TGATCA 2 cut(s) 490, 622
FblI GTMKAC 1 cut(s) 241
Fnu4HI GCNGC 7 cut(s) 222, 225, 1550, 2018, 2265, 2355, 2553
Fsp4HI GCNGC 7 cut(s) 222, 225, 1550, 2018, 2265, 2355, 2553
FspBI CTAG 5 cut(s) 918, 1145, 1443, 1526, 2240
FspI TGCGCA 1 cut(s) 36
GlaI GCGC 2 cut(s) 26, 36
GluI GCNGC 7 cut(s) 222, 225, 1550, 2018, 2265, 2355, 2553
GsaI CCCAGC 4 cut(s) 1958, 2024, 2210, 2295
GsuI CTGGAG 1 cut(s) 1985
HaeIII GGCC 4 cut(s) 950, 1034, 1513, 1681
HapII CCGG 2 cut(s) 911, 1678
HhaI GCGC 2 cut(s) 27, 37
Hin6I GCGC 2 cut(s) 25, 35
HinP1I GCGC 2 cut(s) 25, 35
HinfI GANTC 9 cut(s) 511, 1448, 1629, 1880, 2003, 2360, 2426, 2639, 2645
HpaII CCGG 2 cut(s) 911, 1678
HphI GGTGA 6 cut(s) 602, 1127, 1436, 1661, 1666, 2627
Hpy166II GTNNAC 3 cut(s) 242, 250, 402
Hpy188III TCNNGA 9 cut(s) 108, 124, 902, 1673, 1884, 1925, 1964, 2576, 2582
Hpy8I GTNNAC 3 cut(s) 242, 250, 402
Hpy99I CGWCG 1 cut(s) 855
HpyAV CCTTC 7 cut(s) 410, 671, 869, 1033, 1089, 1503, 2126
HpyCH4IV ACGT 3 cut(s) 244, 817, 850
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 1991, 2156, 2273
HpyF3I CTNAG 8 cut(s) 8, 106, 405, 744, 1769, 2466, 2490, 2641
HpySE526I ACGT 3 cut(s) 244, 817, 850
HspAI GCGC 2 cut(s) 25, 35
KpnI GGTACC 1 cut(s) 911
Ksp22I TGATCA 2 cut(s) 490, 622
Lsp1109I GCAGC 6 cut(s) 208, 236, 1536, 2029, 2276, 2341
LweI GCATC 6 cut(s) 79, 535, 1528, 1891, 2116, 2235
MaeI CTAG 5 cut(s) 918, 1145, 1443, 1526, 2240
MaeII ACGT 3 cut(s) 244, 817, 850
MaeIII GTNAC 5 cut(s) 135, 196, 296, 442, 1654
MbiI CCGCTC 1 cut(s) 2251
MboII GAAGA 8 cut(s) 1279, 1482, 2075, 2106, 2231, 2555, 2558, 2618
MfeI CAATTG 2 cut(s) 565, 2154
MflI RGATCY 8 cut(s) 41, 64, 87, 614, 1160, 1921, 2371, 2572
MlyI GAGTC 4 cut(s) 1623, 2435, 2633, 2639
MmeI TCCRAC 6 cut(s) 98, 350, 833, 1300, 1859, 1875
Mph1103I ATGCAT 1 cut(s) 2228
MseI TTAA 6 cut(s) 21, 276, 450, 605, 1398, 2343
MslI CAYNNNNRTG 4 cut(s) 440, 1302, 1860, 2538
MspA1I CMGCKG 4 cut(s) 1954, 2020, 2233, 2465
MspI CCGG 2 cut(s) 911, 1678
MspR9I CCNGG 3 cut(s) 1259, 1624, 2681
MunI CAATTG 2 cut(s) 565, 2154
Mva1269I GAATGC 5 cut(s) 421, 769, 1370, 2186, 2228
MvaI CCWGG 3 cut(s) 1259, 1624, 2681
MwoI GCNNNNNNNGC 4 cut(s) 10, 1991, 2156, 2273
NcoI CCATGG 3 cut(s) 83, 144, 264
NdeI CATATG 1 cut(s) 337
NheI GCTAGC 1 cut(s) 917
NlaIV GGNNCC 6 cut(s) 89, 112, 880, 909, 1051, 2677
NmeAIII GCCGAG 1 cut(s) 1151
NmuCI GTSAC 4 cut(s) 135, 296, 442, 1654
NsbI TGCGCA 1 cut(s) 36
NsiI ATGCAT 1 cut(s) 2228
NspI RCATGY 3 cut(s) 238, 364, 2533
PaeI GCATGC 1 cut(s) 2533
PaeR7I CTCGAG 2 cut(s) 1884, 2423
PagI TCATGA 1 cut(s) 2581
PciI ACATGT 1 cut(s) 234
PctI GAATGC 5 cut(s) 421, 769, 1370, 2186, 2228
PfeI GAWTC 5 cut(s) 511, 1448, 1880, 2003, 2360
PfoI TCCNGGA 1 cut(s) 1257
PinAI ACCGGT 1 cut(s) 910
PkrI GCNGC 7 cut(s) 223, 226, 1551, 2019, 2266, 2356, 2554
PleI GAGTC 4 cut(s) 1623, 2434, 2633, 2639
PpsI GAGTC 4 cut(s) 1623, 2434, 2633, 2639
Ppu21I YACGTR 1 cut(s) 245
PscI ACATGT 1 cut(s) 234
PsiI TTATAA 2 cut(s) 731, 1611
Psp6I CCWGG 3 cut(s) 1257, 1622, 2679
PspEI GGTNACC 1 cut(s) 1654
PspFI CCCAGC 4 cut(s) 1954, 2020, 2206, 2291
PspGI CCWGG 3 cut(s) 1257, 1622, 2679
PspN4I GGNNCC 6 cut(s) 89, 112, 880, 909, 1051, 2677
PspPI GGNCC 3 cut(s) 948, 1675, 1680
PspXI VCTCGAGB 1 cut(s) 2423
PstI CTGCAG 3 cut(s) 226, 703, 2359
PstNI CAGNNNCTG 1 cut(s) 1925
PsuI RGATCY 8 cut(s) 41, 64, 87, 614, 1160, 1921, 2371, 2572
PvuII CAGCTG 4 cut(s) 1954, 2020, 2233, 2465
RsaI GTAC 8 cut(s) 403, 602, 909, 1127, 1688, 1784, 2474, 2622
RsaNI GTAC 8 cut(s) 402, 601, 908, 1126, 1687, 1783, 2473, 2621
RseI CAYNNNNRTG 4 cut(s) 440, 1302, 1860, 2538
SaqAI TTAA 6 cut(s) 21, 276, 450, 605, 1398, 2343
SatI GCNGC 7 cut(s) 222, 225, 1550, 2018, 2265, 2355, 2553
Sau96I GGNCC 3 cut(s) 948, 1675, 1680
SchI GAGTC 4 cut(s) 1623, 2435, 2633, 2639
ScrFI CCNGG 3 cut(s) 1259, 1624, 2681
SfaNI GCATC 6 cut(s) 79, 535, 1528, 1891, 2116, 2235
SfcI CTRYAG 4 cut(s) 222, 699, 1917, 2355
Sfr274I CTCGAG 2 cut(s) 1884, 2423
SinI GGWCC 1 cut(s) 1675
SlaI CTCGAG 2 cut(s) 1884, 2423
SmiMI CAYNNNNRTG 4 cut(s) 440, 1302, 1860, 2538
SmlI CTYRAG 2 cut(s) 1884, 2423
SmoI CTYRAG 2 cut(s) 1884, 2423
SphI GCATGC 1 cut(s) 2533
SsiI CCGC 3 cut(s) 1422, 2251, 2553
SspI AATATT 1 cut(s) 1830
SspMI CTAG 5 cut(s) 918, 1145, 1443, 1526, 2240
StyD4I CCNGG 3 cut(s) 1257, 1622, 2679
StyI CCWWGG 3 cut(s) 83, 144, 264
TaiI ACGT 3 cut(s) 247, 820, 853
TaqI TCGA 4 cut(s) 901, 1731, 1885, 2424
TaqII GACCGA 1 cut(s) 171
TatI WGTACW 5 cut(s) 401, 1125, 1686, 1782, 2620
TauI GCSGC 1 cut(s) 2555
TfiI GAWTC 5 cut(s) 511, 1448, 1880, 2003, 2360
Tru1I TTAA 6 cut(s) 21, 276, 450, 605, 1398, 2343
Tru9I TTAA 6 cut(s) 21, 276, 450, 605, 1398, 2343
TscAI CASTG 8 cut(s) 301, 502, 589, 634, 1510, 1867, 1911, 2443
TseFI GTSAC 4 cut(s) 135, 296, 442, 1654
TseI GCWGC 6 cut(s) 221, 224, 1549, 2017, 2264, 2354
Tsp45I GTSAC 4 cut(s) 135, 296, 442, 1654
TspDTI ATGAA 7 cut(s) 854, 975, 1440, 1536, 1658, 2556, 2666
TspRI CASTG 8 cut(s) 301, 502, 589, 634, 1510, 1867, 1911, 2443
VpaK11BI GGWCC 1 cut(s) 1675
XagI CCTNNNNNAGG 1 cut(s) 2336
XapI RAATTY 5 cut(s) 191, 532, 1072, 1239, 1934
XceI RCATGY 3 cut(s) 238, 364, 2533
XcmI CCANNNNNNNNNTGG 1 cut(s) 81
XhoI CTCGAG 2 cut(s) 1884, 2423
XmiI GTMKAC 1 cut(s) 241
XspI CTAG 5 cut(s) 918, 1145, 1443, 1526, 2240
Zsp2I ATGCAT 1 cut(s) 2228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.