pycom02g07740

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012977
Physical Location & Seq
Reverse (-)
5451977 .. 5452549
573 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g07740.1

Sequence Viewer

Length: 573 bp
ATGAACGTGTTGGATTCTAATCTCGAGGCTCTTGCTGTCAATTACTTGAGCTTTGGATTGTTCACGACAACTTGGGTCGCCGTTTTGACCGCCGCCATTAGCTTCTGGAGGCTCAGACCCAGAGCCGCCGCTTCCACCAGCAGTGCCATCACTTCTCCTTCATCTTCTTCATTGAGTTTCCGCTCACAACCTGCCGTTTCCGACCTCAGCTTGCATGGGTCGTCGCCGGTTTCCGAGATAACGCAGTCAGATGCGGCGGCGAAAAAGGTGGACCCGCCATGCTCTGTTCCATCATTGGCCAAGTTCAATGACAGCAGAGTGCAGCTGACAAAGGGTTCGAAGTTTGCGGTGTACTTTGAGGATAGTAACGTTGAGAGTGACATTGACCTGGCCGCGACCTCATCGCTGATGGCGGAGGAGGATGAGATGGAAGGGAGCGGCAGCGATGAGGTGGTGGTGGTGGCTAATTGGTGGGATGATGTGTTGAGGTTGAGAACGGGGGAGATGGTTTGGTACAGGTTCCAGGACTTGACGGAGCTAAACGGAAACGTCGTTAGATTATGGGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.74

Weight (kDa)

4.4

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012977)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43110 AT5G20790 AT5G20790
fragaria_vesca FvH4_1g09230
malus_domestica MD02G1099300.v1.1 MD15G1222300.v1.1
prunus_persica Prupe.7G193600_v2.0.a1
pyrus_communis pycom02g07740
rosa_chinensis RchiOBHm_Chr2g0095761
rosa_laevigata RLG00000016590
rosa_multiflora Rmu_sc0005941.1_g000003
rosa_roxburghii Rroxscaffold_2G00146120
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101400 Rh2BG103100 Rh2CG105400 Rh2DG102800
rosa_wichuraiana Rw2G007690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 199
AccBSI CCGCTC 2 cut(s) 183, 438
AccII CGCG 1 cut(s) 395
AclI AACGTT 1 cut(s) 369
AcoI YGGCCR 2 cut(s) 297, 390
AfaI GTAC 2 cut(s) 353, 515
AflIII ACRYGT 1 cut(s) 6
AgsI TTSAA 1 cut(s) 307
AjnI CCWGG 2 cut(s) 387, 522
AluBI AGCT 5 cut(s) 51, 102, 210, 325, 538
AluI AGCT 5 cut(s) 51, 102, 210, 325, 538
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 2 cut(s) 297, 390
ApeKI GCWGC 2 cut(s) 322, 441
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
AspS9I GGNCC 1 cut(s) 271
AsuII TTCGAA 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 1 cut(s) 271
BalI TGGCCA 1 cut(s) 299
BbvCI CCTCAGC 1 cut(s) 206
BbvI GCAGC 2 cut(s) 334, 453
BccI CCATC 5 cut(s) 155, 298, 403, 421, 499
BceAI ACGGC 2 cut(s) 65, 179
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 2 cut(s) 389, 524
BfuAI ACCTGC 1 cut(s) 199
BisI GCNGC 9 cut(s) 93, 126, 129, 255, 258, 323, 393, 439, 442
BlsI GCNGC 9 cut(s) 94, 127, 130, 256, 259, 324, 394, 440, 443
Bme1390I CCNGG 2 cut(s) 389, 524
Bme18I GGWCC 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 2 cut(s) 273, 521
BmrFI CCNGG 2 cut(s) 389, 524
BmsI GCATC 1 cut(s) 241
BpmI CTGGAG 1 cut(s) 127
Bpu10I CCTNAGC 1 cut(s) 206
Bpu14I TTCGAA 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 18
Bse118I RCCGGY 1 cut(s) 226
Bse8I GATNNNNATC 1 cut(s) 18
BseBI CCWGG 2 cut(s) 389, 524
BseGI GGATG 3 cut(s) 427, 481, 571
BseJI GATNNNNATC 1 cut(s) 18
BseMII CTCAG 2 cut(s) 127, 220
BseRI GAGGAG 1 cut(s) 431
BseXI GCAGC 2 cut(s) 334, 453
BsgI GTGCAG 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 395
BshFI GGCC 2 cut(s) 299, 392
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 1 cut(s) 227
BsnI GGCC 2 cut(s) 299, 392
BsoBI CYCGRG 1 cut(s) 23
Bsp119I TTCGAA 1 cut(s) 338
BspANI GGCC 2 cut(s) 299, 392
BspCNI CTCAG 2 cut(s) 126, 219
BspFNI CGCG 1 cut(s) 395
BspLI GGNNCC 2 cut(s) 273, 521
BspMI ACCTGC 1 cut(s) 199
BspT104I TTCGAA 1 cut(s) 338
BsrBI CCGCTC 2 cut(s) 183, 438
BsrFI RCCGGY 1 cut(s) 226
BssAI RCCGGY 1 cut(s) 226
Bst2UI CCWGG 2 cut(s) 389, 524
BstBI TTCGAA 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 212
BstDEI CTNAG 2 cut(s) 113, 206
BstF5I GGATG 3 cut(s) 427, 481, 571
BstFNI CGCG 1 cut(s) 395
BstNI CCWGG 2 cut(s) 389, 524
BstSCI CCNGG 2 cut(s) 387, 522
BstUI CGCG 1 cut(s) 395
BstV1I GCAGC 2 cut(s) 334, 453
BsuRI GGCC 2 cut(s) 299, 392
BtgZI GCGATG 2 cut(s) 387, 459
BtsCI GGATG 3 cut(s) 427, 481, 571
BtsI GCAGTG 1 cut(s) 148
BtsIMutI CAGTG 1 cut(s) 148
BveI ACCTGC 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 1 cut(s) 271
Csp6I GTAC 2 cut(s) 352, 514
CviAII CATG 2 cut(s) 215, 279
CviQI GTAC 2 cut(s) 352, 514
DdeI CTNAG 2 cut(s) 113, 206
EaeI YGGCCR 2 cut(s) 297, 390
EciI GGCGGA 1 cut(s) 428
Eco47I GGWCC 1 cut(s) 271
Eco88I CYCGRG 1 cut(s) 23
EcoRII CCWGG 2 cut(s) 387, 522
FaeI CATG 2 cut(s) 218, 282
FaiI YATR 3 cut(s) 216, 280, 562
FatI CATG 2 cut(s) 214, 278
FauI CCCGC 1 cut(s) 282
Fnu4HI GCNGC 9 cut(s) 93, 126, 129, 255, 258, 323, 393, 439, 442
FokI GGATG 2 cut(s) 434, 488
Fsp4HI GCNGC 9 cut(s) 93, 126, 129, 255, 258, 323, 393, 439, 442
GluI GCNGC 9 cut(s) 93, 126, 129, 255, 258, 323, 393, 439, 442
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 2 cut(s) 299, 392
HapII CCGG 1 cut(s) 227
Hin1II CATG 2 cut(s) 218, 282
HinfI GANTC 1 cut(s) 14
HpaII CCGG 1 cut(s) 227
Hpy166II GTNNAC 3 cut(s) 63, 271, 352
Hpy188I TCNGA 4 cut(s) 116, 202, 235, 250
Hpy188III TCNNGA 3 cut(s) 23, 64, 106
Hpy8I GTNNAC 3 cut(s) 63, 271, 352
Hpy99I CGWCG 2 cut(s) 226, 554
HpyAV CCTTC 2 cut(s) 168, 425
HpyCH4IV ACGT 3 cut(s) 6, 369, 549
HpyCH4V TGCA 2 cut(s) 214, 322
HpyF3I CTNAG 2 cut(s) 113, 206
HpySE526I ACGT 3 cut(s) 6, 369, 549
Hsp92II CATG 2 cut(s) 218, 282
LmnI GCTCC 2 cut(s) 435, 535
Lsp1109I GCAGC 2 cut(s) 334, 453
LweI GCATC 1 cut(s) 241
MaeII ACGT 3 cut(s) 6, 369, 549
MaeIII GTNAC 2 cut(s) 365, 377
MbiI CCGCTC 2 cut(s) 183, 438
MboII GAAGA 2 cut(s) 156, 159
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 2 cut(s) 40, 466
MluNI TGGCCA 1 cut(s) 299
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 9 cut(s) 19, 102, 215, 352, 409, 409, 412, 442, 480
Mox20I TGGCCA 1 cut(s) 299
MscI TGGCCA 1 cut(s) 299
Msp20I TGGCCA 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 325
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 389, 524
MvaI CCWGG 2 cut(s) 389, 524
MvnI CGCG 1 cut(s) 395
NlaIII CATG 2 cut(s) 218, 282
NlaIV GGNNCC 2 cut(s) 273, 521
NmuCI GTSAC 1 cut(s) 377
NspV TTCGAA 1 cut(s) 338
PaeR7I CTCGAG 1 cut(s) 23
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PfeI GAWTC 1 cut(s) 14
PfoI TCCNGGA 1 cut(s) 522
PkrI GCNGC 9 cut(s) 94, 127, 130, 256, 259, 324, 394, 440, 443
Psp1406I AACGTT 1 cut(s) 369
Psp6I CCWGG 2 cut(s) 387, 522
PspGI CCWGG 2 cut(s) 387, 522
PspN4I GGNNCC 2 cut(s) 273, 521
PspPI GGNCC 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 2 cut(s) 353, 515
RsaNI GTAC 2 cut(s) 352, 514
SatI GCNGC 9 cut(s) 93, 126, 129, 255, 258, 323, 393, 439, 442
Sau96I GGNCC 1 cut(s) 271
ScrFI CCNGG 2 cut(s) 389, 524
SfaNI GCATC 1 cut(s) 241
Sfr274I CTCGAG 1 cut(s) 23
SfuI TTCGAA 1 cut(s) 338
SinI GGWCC 1 cut(s) 271
SlaI CTCGAG 1 cut(s) 23
SmlI CTYRAG 2 cut(s) 23, 46
SmoI CTYRAG 2 cut(s) 23, 46
Sse9I AATT 2 cut(s) 40, 466
StyD4I CCNGG 2 cut(s) 387, 522
TaiI ACGT 3 cut(s) 9, 372, 552
TaqI TCGA 2 cut(s) 24, 338
TasI AATT 2 cut(s) 40, 466
TatI WGTACW 1 cut(s) 351
TauI GCSGC 7 cut(s) 95, 128, 131, 257, 260, 395, 441
TfiI GAWTC 1 cut(s) 14
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 377
TseI GCWGC 2 cut(s) 322, 441
Tsp45I GTSAC 1 cut(s) 377
TspDTI ATGAA 3 cut(s) 17, 150, 159
TspGWI ACGGA 2 cut(s) 548, 558
TspRI CASTG 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 271
XhoI CTCGAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.