MD15G1222300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012977
Physical Location & Seq
Reverse (-)
18036664 .. 18037290
627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1222300.v1.1.491

Sequence Viewer

Length: 627 bp
ATGAACGTGTTGGATTCTAATCTCGAAGCTCTTGCTTTCAATTACTTGAGCTTCGGATTGTTCACTGTTGTGAACAATCTGTGGACTTGGATCGCCGTTTTGACCGCCGCCGTTAGCTTCTGGAGGCTCAGACCTAGAGCCGCCGCCACCAGTGCCATCTCTTCTTCTGCATTGAAATTCCGCTCCCAACCTGCCATTTCCGGCCTCAGCTTAAATGGGTCGTCGCCGGTTCCCGAGATAACTCAGTCAAATTCGGCGGCGAAGAAGGTGGAGCCACCATGCTTTGTTCCGGCATTGGCTAAGTTCGGTGACAGCACAGTGCAGCTGACAAAGGGTTCGAAGTTTGCGGTGTATTTTGAGGATAGTGACGTTGAGAGTGACCTGACAGCGACCTCATCACTGACGCCGGAGAAGGATGAGACGGAAGAAAGCGGCAGCGATGAGGTGGCGGCGGCTAGTTGGTGGGAGGATGTTTTGACGTTGAGAACGGGAGAGATGAGTTGGTACAGGTACCAGGACTTGACGGAGCTTAACGGTAACGTCGTTAGATTATGGGATGATTACAGTAGAATAGTTGGTGAAACCGCGTCCAGCTCAGCAAAACCAAAGCGACGTGTCGTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

22.86

Weight (kDa)

4.8

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012977)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43110 AT5G20790 AT5G20790
fragaria_vesca FvH4_1g09230
malus_domestica MD02G1099300.v1.1 MD15G1222300.v1.1
prunus_persica Prupe.7G193600_v2.0.a1
pyrus_communis pycom02g07740
rosa_chinensis RchiOBHm_Chr2g0095761
rosa_laevigata RLG00000016590
rosa_multiflora Rmu_sc0005941.1_g000003
rosa_roxburghii Rroxscaffold_2G00146120
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101400 Rh2BG103100 Rh2CG105400 Rh2DG102800
rosa_wichuraiana Rw2G007690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 199
Acc65I GGTACC 1 cut(s) 510
AccB1I GGYRCC 1 cut(s) 510
AccBSI CCGCTC 1 cut(s) 183
AccII CGCG 1 cut(s) 587
AclWI GGATC 1 cut(s) 98
AcsI RAATTY 2 cut(s) 176, 250
AcyI GRCGYC 1 cut(s) 404
AfaI GTAC 2 cut(s) 506, 512
AflIII ACRYGT 2 cut(s) 6, 613
AgsI TTSAA 2 cut(s) 40, 175
AjiI CACGTC 1 cut(s) 614
AjnI CCWGG 1 cut(s) 513
AleI CACNNNNGTG 1 cut(s) 68
AluBI AGCT 7 cut(s) 29, 51, 117, 210, 325, 529, 594
AluI AGCT 7 cut(s) 29, 51, 117, 210, 325, 529, 594
Alw26I GTCTC 1 cut(s) 413
AlwI GGATC 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 2 cut(s) 322, 435
ApoI RAATTY 2 cut(s) 176, 250
ArsI GACNNNNNNTTYG 3 cut(s) 319, 351, 603
Asp718I GGTACC 1 cut(s) 510
AsuHPI GGTGA 2 cut(s) 320, 590
AsuII TTCGAA 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 233
BanI GGYRCC 1 cut(s) 510
BbvCI CCTCAGC 1 cut(s) 206
BbvI GCAGC 2 cut(s) 334, 447
BccI CCATC 1 cut(s) 164
BceAI ACGGC 2 cut(s) 80, 95
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 1 cut(s) 515
BcoDI GTCTC 1 cut(s) 413
BfaI CTAG 2 cut(s) 135, 456
BfuAI ACCTGC 1 cut(s) 199
BisI GCNGC 9 cut(s) 108, 141, 144, 258, 323, 433, 436, 450, 453
BlpI GCTNAGC 1 cut(s) 595
BlsI GCNGC 9 cut(s) 109, 142, 145, 259, 324, 434, 437, 451, 454
Bme1390I CCNGG 1 cut(s) 515
BmeT110I CYCGRG 1 cut(s) 233
BmgBI CACGTC 1 cut(s) 614
BmiI GGNNCC 3 cut(s) 231, 273, 512
BmrFI CCNGG 1 cut(s) 515
BpmI CTGGAG 1 cut(s) 142
Bpu10I CCTNAGC 1 cut(s) 206
Bpu1102I GCTNAGC 1 cut(s) 595
Bpu14I TTCGAA 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 18
BsaHI GRCGYC 1 cut(s) 404
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 150
Bse8I GATNNNNATC 1 cut(s) 18
BseBI CCWGG 1 cut(s) 515
BseGI GGATG 3 cut(s) 421, 475, 562
BseJI GATNNNNATC 1 cut(s) 18
BseMII CTCAG 4 cut(s) 142, 220, 257, 609
BseNI ACTGG 1 cut(s) 150
BseXI GCAGC 2 cut(s) 334, 447
BsgI GTGCAG 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 587
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 510
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 4 cut(s) 201, 227, 290, 407
BsmAI GTCTC 1 cut(s) 413
BsmBI CGTCTC 1 cut(s) 413
BsnI GGCC 1 cut(s) 204
BsoBI CYCGRG 1 cut(s) 233
Bsp119I TTCGAA 1 cut(s) 338
Bsp143I GATC 1 cut(s) 90
Bsp1720I GCTNAGC 1 cut(s) 595
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 4 cut(s) 141, 219, 256, 608
BspFNI CGCG 1 cut(s) 587
BspLI GGNNCC 3 cut(s) 231, 273, 512
BspMI ACCTGC 1 cut(s) 199
BspPI GGATC 1 cut(s) 98
BspT104I TTCGAA 1 cut(s) 338
BspT107I GGYRCC 1 cut(s) 510
BsrBI CCGCTC 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 1 cut(s) 150
BssAI RCCGGY 1 cut(s) 226
BssMI GATC 1 cut(s) 90
BssNI GRCGYC 1 cut(s) 404
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 4 cut(s) 67, 319, 536, 566
Bst6I CTCTTC 1 cut(s) 166
BstACI GRCGYC 1 cut(s) 404
BstBI TTCGAA 1 cut(s) 338
BstDEI CTNAG 5 cut(s) 128, 206, 243, 300, 595
BstF5I GGATG 3 cut(s) 421, 475, 562
BstFNI CGCG 1 cut(s) 587
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 1 cut(s) 90
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNI CCWGG 1 cut(s) 515
BstSCI CCNGG 1 cut(s) 513
BstUI CGCG 1 cut(s) 587
BstV1I GCAGC 2 cut(s) 334, 447
BsuRI GGCC 1 cut(s) 204
BtgZI GCGATG 1 cut(s) 453
BtrI CACGTC 1 cut(s) 614
BtsCI GGATG 3 cut(s) 421, 475, 562
BtsIMutI CAGTG 4 cut(s) 63, 157, 324, 398
BveI ACCTGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 226
CseI GACGC 2 cut(s) 412, 576
Csp6I GTAC 2 cut(s) 505, 511
CviAII CATG 1 cut(s) 279
CviQI GTAC 2 cut(s) 505, 511
DdeI CTNAG 5 cut(s) 128, 206, 243, 300, 595
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 166
Eco88I CYCGRG 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 513
Esp3I CGTCTC 1 cut(s) 413
FaeI CATG 1 cut(s) 282
FaiI YATR 2 cut(s) 280, 553
FatI CATG 1 cut(s) 278
Fnu4HI GCNGC 9 cut(s) 108, 141, 144, 258, 323, 433, 436, 450, 453
FokI GGATG 3 cut(s) 428, 482, 569
Fsp4HI GCNGC 9 cut(s) 108, 141, 144, 258, 323, 433, 436, 450, 453
FspBI CTAG 2 cut(s) 135, 456
GluI GCNGC 9 cut(s) 108, 141, 144, 258, 323, 433, 436, 450, 453
GsuI CTGGAG 1 cut(s) 142
HaeIII GGCC 1 cut(s) 204
HapII CCGG 4 cut(s) 201, 227, 290, 407
HgaI GACGC 2 cut(s) 412, 576
Hin1I GRCGYC 1 cut(s) 404
Hin1II CATG 1 cut(s) 282
HinfI GANTC 1 cut(s) 14
HpaII CCGG 4 cut(s) 201, 227, 290, 407
HphI GGTGA 2 cut(s) 320, 590
Hpy166II GTNNAC 3 cut(s) 63, 73, 84
Hpy188I TCNGA 2 cut(s) 56, 131
Hpy188III TCNNGA 3 cut(s) 23, 121, 233
Hpy8I GTNNAC 3 cut(s) 63, 73, 84
Hpy99I CGWCG 3 cut(s) 226, 545, 615
HpyAV CCTTC 2 cut(s) 259, 406
HpyCH4III ACNGT 4 cut(s) 67, 319, 536, 566
HpyCH4IV ACGT 5 cut(s) 6, 369, 479, 540, 613
HpyCH4V TGCA 2 cut(s) 170, 322
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 5 cut(s) 128, 206, 243, 300, 595
HpySE526I ACGT 5 cut(s) 6, 369, 479, 540, 613
Hsp92I GRCGYC 1 cut(s) 404
Hsp92II CATG 1 cut(s) 282
KpnI GGTACC 1 cut(s) 514
Kzo9I GATC 1 cut(s) 90
LmnI GCTCC 3 cut(s) 188, 271, 526
Lsp1109I GCAGC 2 cut(s) 334, 447
MaeI CTAG 2 cut(s) 135, 456
MaeII ACGT 5 cut(s) 6, 369, 479, 540, 613
MaeIII GTNAC 4 cut(s) 308, 365, 377, 536
MalI GATC 1 cut(s) 92
MbiI CCGCTC 1 cut(s) 183
MboI GATC 1 cut(s) 90
MboII GAAGA 4 cut(s) 153, 156, 274, 437
MluCI AATT 3 cut(s) 40, 176, 250
MnlI CCTC 6 cut(s) 117, 215, 352, 403, 436, 460
MseI TTAA 2 cut(s) 212, 531
MslI CAYNNNNRTG 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 325
MspI CCGG 4 cut(s) 201, 227, 290, 407
MspR9I CCNGG 1 cut(s) 515
MvaI CCWGG 1 cut(s) 515
MvnI CGCG 1 cut(s) 587
MwoI GCNNNNNNNGC 1 cut(s) 152
NdeII GATC 1 cut(s) 90
NlaIII CATG 1 cut(s) 282
NlaIV GGNNCC 3 cut(s) 231, 273, 512
NmuCI GTSAC 3 cut(s) 308, 365, 377
NspV TTCGAA 1 cut(s) 338
OliI CACNNNNGTG 1 cut(s) 68
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 9 cut(s) 109, 142, 145, 259, 324, 434, 437, 451, 454
Psp6I CCWGG 1 cut(s) 513
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 3 cut(s) 231, 273, 512
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 2 cut(s) 506, 512
RsaNI GTAC 2 cut(s) 505, 511
RseI CAYNNNNRTG 1 cut(s) 68
SaqAI TTAA 2 cut(s) 212, 531
SatI GCNGC 9 cut(s) 108, 141, 144, 258, 323, 433, 436, 450, 453
Sau3AI GATC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 515
SfuI TTCGAA 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 3 cut(s) 40, 176, 250
SspMI CTAG 2 cut(s) 135, 456
StyD4I CCNGG 1 cut(s) 513
TaaI ACNGT 4 cut(s) 67, 319, 536, 566
TaiI ACGT 5 cut(s) 9, 372, 482, 543, 616
TaqI TCGA 2 cut(s) 24, 338
TasI AATT 3 cut(s) 40, 176, 250
TauI GCSGC 7 cut(s) 110, 143, 146, 260, 435, 452, 455
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 2 cut(s) 212, 531
Tru9I TTAA 2 cut(s) 212, 531
TscAI CASTG 4 cut(s) 70, 157, 324, 405
TseFI GTSAC 3 cut(s) 308, 365, 377
TseI GCWGC 2 cut(s) 322, 435
Tsp45I GTSAC 3 cut(s) 308, 365, 377
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 437, 539
TspRI CASTG 4 cut(s) 70, 157, 324, 405
XapI RAATTY 2 cut(s) 176, 250
XspI CTAG 2 cut(s) 135, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.