MD02G1177500.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011781
Physical Location & Seq
Reverse (-)
15663260 .. 15665127
1868 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1177500.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGCCGTTGATTTCATGGGTTACAGAAACACCATCAGTAGCAGCAGCAGCTTCTCCGCCAAGTTGGAAGAGAACGCCGTGCAGGAAGCGGCTTCCGGCCTCGAGAGCGTCGAGAAGCTTATTCGCTTGCTGTCCCAGGCACAGCAGAACCAGCACCAAGGGAAATATCCGTCGACGGCTATGGACATGGACTGCAGAGCCGTCGCGGACGTCGCTGTTTCCAAGTTCAAGAAGGTCATTTCTCTTCTTGGTCGGACCCGGACCGGCCACGCCCGGTTCCGGCGAGCCCCTTTGACTTTGAGTTCCGGATCGTCTTCTCAAACCCAAAACCAGAGCCAAGAGATCCTCGTCAAGCATGTTCCGTTACCGTTAGAGTCCACTAAGGTTTACCATGCGACGCCGATCCAGCAGATCCCGCCACCTCACCACCACCACAGTACGGTGCTTGAGAGCACTAAGGACTCATCTACCACTATAAATTTCTCATATCCAACTACGACGTCGTTTATATCGTCGTTGACCGGAGACTCCGATAGCAAGCAGCCAATGTCGTCATCGTCTTTTCAAATTACCAATTTGTCCCAGGTTTCCTCGGCCGGAAAGCCGCCGCTTTCCTCCGCCTCGTTGAAGCGGAAGTGCAGCTCCGAGAACTTGGGGTCTGGGAAGTGCGGTGCTGGGTCCTCCGGCCGCTGCCATTGCAAGAAGAGAAAGCTGAGACAGAAGAGGATCGTGAGAGTTCCGGCTATAAGCTTGAAGTTGGCCGATATCCCACCTGACGATTACTCCTGGAGAAAGTACGGACAAAAACCCATCAAAGGATCTCCACATCCAAGGGGATACTACAAATGCAGCAGTGTAAGAGGATGCCCGGCTCGAAAACACGTAGAGAGAGCTCTGGACGATGCGGCAATGCTAGTGGTTACCTACGAAGGCGAGCACAATCACTCTCTCTCCGTTGCAGAGACCTCCAATCTTATTCTAGAATCTTCTTAG

Protein Analysis

331

Amino Acids

35.97

Weight (kDa)

9.65

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_zn_clust PF10533 210 - 255 6.1e-17 Plant zinc cluster domain
WRKY PF03106 259 - 315 2.3e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011781)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23320 AT2G23320
fragaria_vesca FvH4_1g16480 FvH4_1g16480
malus_domestica MD02G1177500.v1.1 MD15G1287300.v1.1
prunus_persica Prupe.6G230600_v2.0.a1
pyrus_communis pycom02g14340 pycom15g25080
rosa_chinensis RchiOBHm_Chr2g0106361
rosa_laevigata RLG00000017492
rosa_multiflora Rmu_sc0016473.1_g000001
rosa_roxburghii Rroxscaffold_2G00137250
rosa_rugosa Rorug02G0135000
rosa_samantha Rh2AG186100 Rh2BG196300 Rh2CG191100 Rh2DG192200
rosa_wichuraiana Rw2G014510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 213, 503
AccI GTMKAC 1 cut(s) 173
AccII CGCG 1 cut(s) 206
AccIII TCCGGA 1 cut(s) 305
AclWI GGATC 6 cut(s) 316, 337, 397, 406, 734, 826
AcoI YGGCCR 5 cut(s) 3, 265, 594, 685, 759
AcsI RAATTY 1 cut(s) 478
AcyI GRCGYC 3 cut(s) 210, 398, 500
AfaI GTAC 2 cut(s) 439, 797
AfiI CCNNNNNNNGG 3 cut(s) 279, 439, 815
AflIII ACRYGT 1 cut(s) 880
AgsI TTSAA 4 cut(s) 229, 566, 628, 754
AjnI CCWGG 3 cut(s) 135, 582, 785
AluBI AGCT 6 cut(s) 51, 118, 642, 712, 750, 893
AluI AGCT 6 cut(s) 51, 118, 642, 712, 750, 893
Alw21I GWGCWC 3 cut(s) 455, 895, 939
Alw26I GTCTC 3 cut(s) 519, 709, 956
AlwI GGATC 6 cut(s) 316, 337, 397, 406, 734, 826
Ama87I CYCGRG 1 cut(s) 101
Aor13HI TCCGGA 1 cut(s) 305
AoxI GGCC 6 cut(s) 3, 97, 265, 594, 685, 759
ApeKI GCWGC 7 cut(s) 42, 45, 48, 541, 639, 690, 849
ApoI RAATTY 1 cut(s) 478
AspS9I GGNCC 3 cut(s) 255, 261, 678
AsuC2I CCSGG 3 cut(s) 259, 274, 869
AsuHPI GGTGA 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 101
AvaII GGWCC 3 cut(s) 255, 261, 678
BanII GRGCYC 2 cut(s) 289, 895
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 3 cut(s) 455, 895, 939
BbvI GCAGC 7 cut(s) 54, 57, 60, 553, 651, 677, 861
BccI CCATC 2 cut(s) 41, 818
BceAI ACGGC 3 cut(s) 62, 185, 192
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BciT130I CCWGG 3 cut(s) 137, 584, 787
BciVI GTATCC 1 cut(s) 830
BcnI CCSGG 3 cut(s) 259, 274, 869
BcoDI GTCTC 3 cut(s) 519, 709, 956
BfaI CTAG 2 cut(s) 914, 980
BfmI CTRYAG 1 cut(s) 193
BfuI GTATCC 1 cut(s) 830
Bme1390I CCNGG 6 cut(s) 137, 259, 274, 584, 787, 869
Bme18I GGWCC 3 cut(s) 255, 261, 678
BmeT110I CYCGRG 1 cut(s) 101
BmgT120I GGNCC 3 cut(s) 255, 261, 678
BmiI GGNNCC 3 cut(s) 257, 278, 679
BmrFI CCNGG 6 cut(s) 137, 259, 274, 584, 787, 869
BmsI GCATC 2 cut(s) 854, 892
BpiI GAAGAC 1 cut(s) 306
BpmI CTGGAG 1 cut(s) 808
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 3 cut(s) 259, 274, 869
BsaAI YACGTR 1 cut(s) 883
BsaHI GRCGYC 3 cut(s) 210, 398, 500
BsaI GGTCTC 1 cut(s) 956
BsaJI CCNNGG 5 cut(s) 135, 157, 582, 591, 830
BsaWI WCCGGW 2 cut(s) 305, 521
BsaXI ACNNNNNCTCC 4 cut(s) 767, 797, 935, 965
Bsc4I CCNNNNNNNGG 3 cut(s) 279, 439, 815
Bse118I RCCGGY 1 cut(s) 263
Bse3DI GCAATG 2 cut(s) 694, 915
BseAI TCCGGA 1 cut(s) 305
BseBI CCWGG 3 cut(s) 137, 584, 787
BseDI CCNNGG 5 cut(s) 135, 157, 582, 591, 830
BseGI GGATG 2 cut(s) 826, 869
BseLI CCNNNNNNNGG 3 cut(s) 279, 439, 815
BseMI GCAATG 2 cut(s) 694, 915
BseMII CTCAG 1 cut(s) 704
BseX3I CGGCCG 2 cut(s) 594, 685
BseXI GCAGC 7 cut(s) 54, 57, 60, 553, 651, 677, 861
BseYI CCCAGC 1 cut(s) 674
BsgI GTGCAG 2 cut(s) 101, 658
Bsh1236I CGCG 1 cut(s) 206
Bsh1285I CGRYCG 2 cut(s) 597, 688
BshFI GGCC 6 cut(s) 5, 99, 267, 596, 687, 761
BsiEI CGRYCG 2 cut(s) 597, 688
BsiHKAI GWGCWC 3 cut(s) 455, 895, 939
BsiHKCI CYCGRG 1 cut(s) 101
BslFI GGGAC 2 cut(s) 118, 565
BslI CCNNNNNNNGG 3 cut(s) 279, 439, 815
BsmAI GTCTC 3 cut(s) 519, 709, 956
BsmFI GGGAC 2 cut(s) 118, 565
BsnI GGCC 6 cut(s) 5, 99, 267, 596, 687, 761
Bso31I GGTCTC 1 cut(s) 956
BsoBI CYCGRG 1 cut(s) 101
Bsp1286I GDGCHC 4 cut(s) 289, 455, 895, 939
Bsp13I TCCGGA 1 cut(s) 305
Bsp143I GATC 6 cut(s) 308, 342, 402, 411, 726, 818
BspANI GGCC 6 cut(s) 5, 99, 267, 596, 687, 761
BspCNI CTCAG 1 cut(s) 705
BspEI TCCGGA 1 cut(s) 305
BspFNI CGCG 1 cut(s) 206
BspLI GGNNCC 3 cut(s) 257, 278, 679
BspMAI CTGCAG 1 cut(s) 197
BspPI GGATC 6 cut(s) 316, 337, 397, 406, 734, 826
BspTNI GGTCTC 1 cut(s) 956
BsrDI GCAATG 2 cut(s) 694, 915
BsrFI RCCGGY 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 5 cut(s) 135, 157, 582, 591, 830
BssMI GATC 6 cut(s) 308, 342, 402, 411, 726, 818
BssNI GRCGYC 3 cut(s) 210, 398, 500
BssT1I CCWWGG 2 cut(s) 157, 830
Bst2UI CCWGG 3 cut(s) 137, 584, 787
Bst4CI ACNGT 3 cut(s) 369, 437, 442
Bst6I CTCTTC 4 cut(s) 63, 249, 698, 716
BstACI GRCGYC 3 cut(s) 210, 398, 500
BstBAI YACGTR 1 cut(s) 883
BstC8I GCNNGC 4 cut(s) 128, 285, 539, 935
BstDEI CTNAG 4 cut(s) 381, 456, 713, 990
BstEII GGTNACC 1 cut(s) 919
BstF5I GGATG 2 cut(s) 826, 869
BstFNI CGCG 1 cut(s) 206
BstKTI GATC 6 cut(s) 311, 345, 405, 414, 729, 821
BstMAI GTCTC 3 cut(s) 519, 709, 956
BstMBI GATC 6 cut(s) 308, 342, 402, 411, 726, 818
BstMCI CGRYCG 2 cut(s) 597, 688
BstMWI GCNNNNNNNGC 7 cut(s) 48, 105, 151, 212, 406, 415, 696
BstNI CCWGG 3 cut(s) 137, 584, 787
BstNSI RCATGY 1 cut(s) 359
BstPI GGTNACC 1 cut(s) 919
BstSCI CCNGG 6 cut(s) 135, 257, 272, 582, 785, 867
BstSFI CTRYAG 1 cut(s) 193
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 7 cut(s) 54, 57, 60, 553, 651, 677, 861
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 3 cut(s) 342, 411, 818
BstYI RGATCY 3 cut(s) 342, 411, 818
BstZI CGGCCG 2 cut(s) 594, 685
BsuI GTATCC 1 cut(s) 830
BsuRI GGCC 6 cut(s) 5, 99, 267, 596, 687, 761
BtsCI GGATG 2 cut(s) 826, 869
BtsI GCAGTG 1 cut(s) 859
BtsIMutI CAGTG 1 cut(s) 859
Cac8I GCNNGC 4 cut(s) 128, 285, 539, 935
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 3 cut(s) 255, 261, 678
CpoI CGGWCCG 1 cut(s) 261
CseI GACGC 2 cut(s) 97, 406
Csp6I GTAC 2 cut(s) 438, 796
CspCI CAANNNNNGTGG 2 cut(s) 897, 932
CspI CGGWCCG 1 cut(s) 261
CviAII CATG 4 cut(s) 16, 187, 356, 392
CviQI GTAC 2 cut(s) 438, 796
DdeI CTNAG 4 cut(s) 381, 456, 713, 990
DpnI GATC 6 cut(s) 310, 344, 404, 413, 728, 820
DpnII GATC 6 cut(s) 308, 342, 402, 411, 726, 818
EaeI YGGCCR 5 cut(s) 3, 265, 594, 685, 759
EagI CGGCCG 2 cut(s) 594, 685
Eam1104I CTCTTC 4 cut(s) 63, 249, 698, 716
EarI CTCTTC 4 cut(s) 63, 249, 698, 716
EciI GGCGGA 2 cut(s) 46, 607
Ecl136II GAGCTC 1 cut(s) 893
EclXI CGGCCG 2 cut(s) 594, 685
Eco130I CCWWGG 2 cut(s) 157, 830
Eco24I GRGCYC 2 cut(s) 289, 895
Eco31I GGTCTC 1 cut(s) 956
Eco32I GATATC 1 cut(s) 766
Eco47I GGWCC 3 cut(s) 255, 261, 678
Eco52I CGGCCG 2 cut(s) 594, 685
Eco53kI GAGCTC 1 cut(s) 893
Eco88I CYCGRG 1 cut(s) 101
Eco91I GGTNACC 1 cut(s) 919
EcoICRI GAGCTC 1 cut(s) 893
EcoO109I RGGNCCY 1 cut(s) 678
EcoO65I GGTNACC 1 cut(s) 919
EcoRII CCWGG 3 cut(s) 135, 582, 785
EcoRV GATATC 1 cut(s) 766
EcoT14I CCWWGG 2 cut(s) 157, 830
EcoT38I GRGCYC 2 cut(s) 289, 895
ErhI CCWWGG 2 cut(s) 157, 830
FaeI CATG 4 cut(s) 19, 190, 359, 395
FaiI YATR 9 cut(s) 17, 182, 188, 357, 393, 476, 487, 509, 746
FaqI GGGAC 2 cut(s) 118, 565
FatI CATG 4 cut(s) 15, 186, 355, 391
FauI CCCGC 1 cut(s) 423
FblI GTMKAC 1 cut(s) 173
FokI GGATG 2 cut(s) 813, 876
FriOI GRGCYC 2 cut(s) 289, 895
FspBI CTAG 2 cut(s) 914, 980
GsaI CCCAGC 1 cut(s) 678
GsuI CTGGAG 1 cut(s) 808
HaeIII GGCC 6 cut(s) 5, 99, 267, 596, 687, 761
HgaI GACGC 2 cut(s) 97, 406
Hin1I GRCGYC 3 cut(s) 210, 398, 500
Hin1II CATG 4 cut(s) 19, 190, 359, 395
HincII GTYRAC 2 cut(s) 174, 519
HindII GTYRAC 2 cut(s) 174, 519
HindIII AAGCTT 2 cut(s) 116, 748
HinfI GANTC 4 cut(s) 374, 461, 527, 983
HphI GGTGA 1 cut(s) 416
Hpy166II GTNNAC 4 cut(s) 174, 378, 388, 519
Hpy188I TCNGA 3 cut(s) 255, 532, 646
Hpy188III TCNNGA 7 cut(s) 103, 112, 229, 306, 730, 896, 980
Hpy8I GTNNAC 4 cut(s) 174, 378, 388, 519
Hpy99I CGWCG 9 cut(s) 113, 175, 178, 206, 215, 400, 502, 505, 517
HpyAV CCTTC 2 cut(s) 226, 923
HpyCH4III ACNGT 3 cut(s) 369, 437, 442
HpyCH4IV ACGT 3 cut(s) 210, 500, 882
HpyCH4V TGCA 6 cut(s) 82, 195, 639, 699, 849, 959
HpyF10VI GCNNNNNNNGC 7 cut(s) 48, 105, 151, 212, 406, 415, 696
HpyF3I CTNAG 4 cut(s) 381, 456, 713, 990
HpySE526I ACGT 3 cut(s) 210, 500, 882
Hsp92I GRCGYC 3 cut(s) 210, 398, 500
Hsp92II CATG 4 cut(s) 19, 190, 359, 395
Kpn2I TCCGGA 1 cut(s) 305
Kzo9I GATC 6 cut(s) 308, 342, 402, 411, 726, 818
LmnI GCTCC 1 cut(s) 647
Lsp1109I GCAGC 7 cut(s) 54, 57, 60, 553, 651, 677, 861
LweI GCATC 2 cut(s) 854, 892
MaeI CTAG 2 cut(s) 914, 980
MaeII ACGT 3 cut(s) 210, 500, 882
MaeIII GTNAC 3 cut(s) 20, 363, 919
MalI GATC 6 cut(s) 310, 344, 404, 413, 728, 820
MboI GATC 6 cut(s) 308, 342, 402, 411, 726, 818
MboII GAAGA 6 cut(s) 80, 236, 306, 715, 733, 978
MflI RGATCY 3 cut(s) 342, 411, 818
MhlI GDGCHC 4 cut(s) 289, 455, 895, 939
MluCI AATT 3 cut(s) 478, 567, 574
MlyI GAGTC 3 cut(s) 383, 455, 521
MmeI TCCRAC 3 cut(s) 45, 233, 515
MroI TCCGGA 1 cut(s) 305
MspA1I CMGCKG 1 cut(s) 690
MspR9I CCNGG 6 cut(s) 137, 259, 274, 584, 787, 869
MvaI CCWGG 3 cut(s) 137, 584, 787
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 7 cut(s) 48, 105, 151, 212, 406, 415, 696
NciI CCSGG 3 cut(s) 259, 274, 869
NdeII GATC 6 cut(s) 308, 342, 402, 411, 726, 818
NlaIII CATG 4 cut(s) 19, 190, 359, 395
NlaIV GGNNCC 3 cut(s) 257, 278, 679
NmeAIII GCCGAG 1 cut(s) 572
NspI RCATGY 1 cut(s) 359
PaeR7I CTCGAG 1 cut(s) 101
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 509
PfeI GAWTC 1 cut(s) 983
PfoI TCCNGGA 1 cut(s) 785
PleI GAGTC 3 cut(s) 382, 455, 521
PpsI GAGTC 3 cut(s) 382, 455, 521
Ppu21I YACGTR 1 cut(s) 883
PpuMI RGGWCCY 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 895
Psp5II RGGWCCY 1 cut(s) 678
Psp6I CCWGG 3 cut(s) 135, 582, 785
PspEI GGTNACC 1 cut(s) 919
PspFI CCCAGC 1 cut(s) 674
PspGI CCWGG 3 cut(s) 135, 582, 785
PspN4I GGNNCC 3 cut(s) 257, 278, 679
PspPI GGNCC 3 cut(s) 255, 261, 678
PspPPI RGGWCCY 1 cut(s) 678
PstI CTGCAG 1 cut(s) 197
PsuI RGATCY 3 cut(s) 342, 411, 818
RsaI GTAC 2 cut(s) 439, 797
RsaNI GTAC 2 cut(s) 438, 796
Rsr2I CGGWCCG 1 cut(s) 261
RsrII CGGWCCG 1 cut(s) 261
SacI GAGCTC 1 cut(s) 895
SalI GTCGAC 1 cut(s) 172
Sau3AI GATC 6 cut(s) 308, 342, 402, 411, 726, 818
Sau96I GGNCC 3 cut(s) 255, 261, 678
SchI GAGTC 3 cut(s) 383, 455, 521
ScrFI CCNGG 6 cut(s) 137, 259, 274, 584, 787, 869
SduI GDGCHC 4 cut(s) 289, 455, 895, 939
SfaNI GCATC 2 cut(s) 854, 892
SfcI CTRYAG 1 cut(s) 193
Sfr274I CTCGAG 1 cut(s) 101
SgrDI CGTCGACG 1 cut(s) 172
SinI GGWCC 3 cut(s) 255, 261, 678
SlaI CTCGAG 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 101, 446
SmoI CTYRAG 2 cut(s) 101, 446
Sse9I AATT 3 cut(s) 478, 567, 574
SspMI CTAG 2 cut(s) 914, 980
SstI GAGCTC 1 cut(s) 895
StyD4I CCNGG 6 cut(s) 135, 257, 272, 582, 785, 867
StyI CCWWGG 2 cut(s) 157, 830
TaaI ACNGT 3 cut(s) 369, 437, 442
TaiI ACGT 3 cut(s) 213, 503, 885
TaqI TCGA 4 cut(s) 102, 111, 173, 874
TasI AATT 3 cut(s) 478, 567, 574
TauI GCSGC 5 cut(s) 92, 607, 610, 690, 908
TfiI GAWTC 1 cut(s) 983
TscAI CASTG 1 cut(s) 859
TseI GCWGC 7 cut(s) 42, 45, 48, 541, 639, 690, 849
TspDTI ATGAA 1 cut(s) 4
TspGWI ACGGA 4 cut(s) 159, 351, 813, 943
TspRI CASTG 1 cut(s) 859
VpaK11BI GGWCC 3 cut(s) 255, 261, 678
XapI RAATTY 1 cut(s) 478
XbaI TCTAGA 1 cut(s) 979
XceI RCATGY 1 cut(s) 359
XhoI CTCGAG 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 173
XspI CTAG 2 cut(s) 914, 980
ZraI GACGTC 2 cut(s) 211, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.