RLG00000017492
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011781
Physical Location & Seq
Reverse (-)
17485795 .. 17487441
1647 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017492

Sequence Viewer

Length: 879 bp
ATGGCCGTGGATTTCATGGGTTATAGGAATTTCTCGGCCAAGCTAGAAGAAAACGCCGTCGTCCAGGAAGCCGCCTCCGGCCTTGAGAGCGTCGAGAAGCTCATCAGATTGCTGTCCTCCCAGCAGAAGCAGGAGAAGTACCACTCCATGGACATGGACATGGACTGCACCGCCGTCGCCGACGTCGCCGTTTCCAAGTTCAAGAAAGTTATTTCTCTTCTGGGCCGGACCCGGACCGGCCACGCCCGCTTCCGGAGAGCCCCTCTCGCCTTCACTACTCCTCCACAAGACTCCGTCAAGCCCATAGATTCTTCGTCGACTAGGGTCTACCATGCGACGCCGATCCAGCAGATCGCTCCGCCGGTGGTTATGCACCACCAGGGCGGCGGGATGGAGGGGACTAAAGACTCGTCCACCACTATAAATTTCTCATCGTACTCTTCTGCTGCGACGACATCGTTTATGTCGTCGTTGACTGGGGACTCCGACAGCAAGCAGCCCCTGTCGTCTTCCGCGTTTCAAATTACCAATGTGTCCCAGGTCTCTTCGGCCGGAAAGCCGCCGCTTTCTTCTGCTTCCATGAAGCGGAAGTGCAGCTCCGACACCTTGGGCTCCGGCAAATGTGGTGCAAGCTCCTCTGGTCGCTGCCATTGCTCAAAGAAAAGAAAGCTGAGATTGAAGAGGGTAGTTAGAGTTCCGGCTATAAGCTTAAAGATGGCTGATATTCCACCGGATGATTACTCTTGGAGAAAGTACGGACAGAAACCCATCAAGGGTTCTCCACATCCAAGGTATAAAATTTTGACCATTTCGATAGAAATCGATGATCTTTCTGCTCAATTACTTCAACTTTTTCTGTGTCCCTTTTTTAATTTGTGA

Protein Analysis

293

Amino Acids

31.88

Weight (kDa)

9.64

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_zn_clust PF10533 195 - 241 5.9e-19 Plant zinc cluster domain
WRKY PF03106 245 - 265 2.1e-06 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011781)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23320 AT2G23320
fragaria_vesca FvH4_1g16480 FvH4_1g16480
malus_domestica MD02G1177500.v1.1 MD15G1287300.v1.1
prunus_persica Prupe.6G230600_v2.0.a1
pyrus_communis pycom02g14340 pycom15g25080
rosa_chinensis RchiOBHm_Chr2g0106361
rosa_laevigata RLG00000017492
rosa_multiflora Rmu_sc0016473.1_g000001
rosa_roxburghii Rroxscaffold_2G00137250
rosa_rugosa Rorug02G0135000
rosa_samantha Rh2AG186100 Rh2BG196300 Rh2CG191100 Rh2DG192200
rosa_wichuraiana Rw2G014510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
AccB7I CCANNNNNTGG 1 cut(s) 148
AccI GTMKAC 2 cut(s) 317, 327
AccII CGCG 1 cut(s) 515
AccIII TCCGGA 1 cut(s) 252
AclWI GGATC 1 cut(s) 337
AcoI YGGCCR 4 cut(s) 3, 36, 238, 549
AcsI RAATTY 3 cut(s) 28, 424, 798
AcyI GRCGYC 2 cut(s) 183, 338
AfaI GTAC 3 cut(s) 140, 437, 755
AfiI CCNNNNNNNGG 4 cut(s) 148, 252, 585, 773
AgsI TTSAA 4 cut(s) 202, 521, 679, 848
AjnI CCWGG 3 cut(s) 63, 378, 537
AluBI AGCT 6 cut(s) 43, 100, 597, 633, 670, 708
AluI AGCT 6 cut(s) 43, 100, 597, 633, 670, 708
Alw26I GTCTC 1 cut(s) 547
AlwI GGATC 1 cut(s) 337
AlwNI CAGNNNCTG 1 cut(s) 502
Aor13HI TCCGGA 1 cut(s) 252
AoxI GGCC 6 cut(s) 3, 36, 79, 223, 238, 549
ApeKI GCWGC 4 cut(s) 446, 496, 594, 645
ApoI RAATTY 3 cut(s) 28, 424, 798
AspS9I GGNCC 3 cut(s) 223, 228, 234
AsuC2I CCSGG 1 cut(s) 232
AvaII GGWCC 2 cut(s) 228, 234
BanII GRGCYC 2 cut(s) 262, 614
BbsI GAAGAC 1 cut(s) 501
BbvI GCAGC 4 cut(s) 433, 508, 606, 632
BccI CCATC 3 cut(s) 385, 709, 776
BceAI ACGGC 3 cut(s) 41, 158, 173
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 3 cut(s) 65, 380, 539
BcnI CCSGG 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 547
BfaI CTAG 2 cut(s) 44, 321
BisI GCNGC 8 cut(s) 72, 385, 447, 497, 560, 563, 595, 646
BlsI GCNGC 8 cut(s) 73, 386, 448, 498, 561, 564, 596, 647
Bme1390I CCNGG 4 cut(s) 65, 232, 380, 539
Bme18I GGWCC 2 cut(s) 228, 234
BmgT120I GGNCC 3 cut(s) 223, 228, 234
BmiI GGNNCC 2 cut(s) 230, 613
BmrFI CCNGG 4 cut(s) 65, 232, 380, 539
BmrI ACTGGG 1 cut(s) 486
BmuI ACTGGG 1 cut(s) 486
BoxI GACNNNNGTC 1 cut(s) 323
BpiI GAAGAC 1 cut(s) 501
BplI GAGNNNNNCTC 4 cut(s) 247, 279, 249, 281
BpuEI CTTGAG 1 cut(s) 104
BpuMI CCSGG 1 cut(s) 232
Bsa29I ATCGAT 1 cut(s) 822
BsaBI GATNNNNATC 1 cut(s) 818
BsaHI GRCGYC 2 cut(s) 183, 338
BsaI GGTCTC 1 cut(s) 547
BsaJI CCNNGG 6 cut(s) 6, 147, 379, 537, 606, 788
BsaWI WCCGGW 2 cut(s) 252, 730
BsaXI ACNNNNNCTCC 2 cut(s) 265, 295
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 252, 585, 773
Bse118I RCCGGY 2 cut(s) 236, 361
Bse1I ACTGG 1 cut(s) 481
Bse3DI GCAATG 1 cut(s) 649
Bse8I GATNNNNATC 1 cut(s) 818
BseAI TCCGGA 1 cut(s) 252
BseBI CCWGG 3 cut(s) 65, 380, 539
BseCI ATCGAT 1 cut(s) 822
BseDI CCNNGG 6 cut(s) 6, 147, 379, 537, 606, 788
BseGI GGATG 3 cut(s) 396, 739, 784
BseJI GATNNNNATC 1 cut(s) 818
BseLI CCNNNNNNNGG 4 cut(s) 148, 252, 585, 773
BseMI GCAATG 1 cut(s) 649
BseMII CTCAG 1 cut(s) 662
BseNI ACTGG 1 cut(s) 481
BseRI GAGGAG 2 cut(s) 270, 625
BseX3I CGGCCG 1 cut(s) 549
BseXI GCAGC 4 cut(s) 433, 508, 606, 632
BseYI CCCAGC 1 cut(s) 120
BsgI GTGCAG 2 cut(s) 151, 613
Bsh1236I CGCG 1 cut(s) 515
Bsh1285I CGRYCG 1 cut(s) 552
BshFI GGCC 6 cut(s) 5, 38, 81, 225, 240, 551
BshVI ATCGAT 1 cut(s) 822
BsiEI CGRYCG 1 cut(s) 552
BslFI GGGAC 4 cut(s) 412, 494, 520, 846
BslI CCNNNNNNNGG 4 cut(s) 148, 252, 585, 773
BsmAI GTCTC 1 cut(s) 547
BsmFI GGGAC 4 cut(s) 412, 494, 520, 846
BsnI GGCC 6 cut(s) 5, 38, 81, 225, 240, 551
Bso31I GGTCTC 1 cut(s) 547
Bsp1286I GDGCHC 2 cut(s) 262, 614
Bsp13I TCCGGA 1 cut(s) 252
Bsp143I GATC 3 cut(s) 342, 351, 826
Bsp19I CCATGG 1 cut(s) 147
BspANI GGCC 6 cut(s) 5, 38, 81, 225, 240, 551
BspCNI CTCAG 1 cut(s) 663
BspDI ATCGAT 1 cut(s) 822
BspEI TCCGGA 1 cut(s) 252
BspFNI CGCG 1 cut(s) 515
BspLI GGNNCC 2 cut(s) 230, 613
BspPI GGATC 1 cut(s) 337
BspTNI GGTCTC 1 cut(s) 547
BsrDI GCAATG 1 cut(s) 649
BsrFI RCCGGY 2 cut(s) 236, 361
BsrI ACTGG 1 cut(s) 481
BssAI RCCGGY 2 cut(s) 236, 361
BssECI CCNNGG 6 cut(s) 6, 147, 379, 537, 606, 788
BssMI GATC 3 cut(s) 342, 351, 826
BssNI GRCGYC 2 cut(s) 183, 338
BssT1I CCWWGG 3 cut(s) 147, 606, 788
Bst2UI CCWGG 3 cut(s) 65, 380, 539
Bst6I CTCTTC 4 cut(s) 222, 445, 550, 674
BstACI GRCGYC 2 cut(s) 183, 338
BstC8I GCNNGC 3 cut(s) 247, 494, 631
BstDEI CTNAG 1 cut(s) 671
BstDSI CCRYGG 2 cut(s) 6, 147
BstF5I GGATG 3 cut(s) 396, 739, 784
BstFNI CGCG 1 cut(s) 515
BstKTI GATC 3 cut(s) 345, 354, 829
BstMAI GTCTC 1 cut(s) 547
BstMBI GATC 3 cut(s) 342, 351, 826
BstMCI CGRYCG 1 cut(s) 552
BstMWI GCNNNNNNNGC 6 cut(s) 87, 185, 246, 266, 346, 651
BstNI CCWGG 3 cut(s) 65, 380, 539
BstPAI GACNNNNGTC 1 cut(s) 323
BstSCI CCNGG 4 cut(s) 63, 230, 378, 537
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 4 cut(s) 433, 508, 606, 632
BstV2I GAAGAC 1 cut(s) 501
BstXI CCANNNNNNTGG 1 cut(s) 154
BstZI CGGCCG 1 cut(s) 549
Bsu15I ATCGAT 1 cut(s) 822
BsuRI GGCC 6 cut(s) 5, 38, 81, 225, 240, 551
BsuTUI ATCGAT 1 cut(s) 822
BtgI CCRYGG 2 cut(s) 6, 147
BtsCI GGATG 3 cut(s) 396, 739, 784
Cac8I GCNNGC 3 cut(s) 247, 494, 631
CaiI CAGNNNCTG 1 cut(s) 502
Cfr10I RCCGGY 2 cut(s) 236, 361
Cfr13I GGNCC 3 cut(s) 223, 228, 234
ClaI ATCGAT 1 cut(s) 822
CpoI CGGWCCG 1 cut(s) 234
CseI GACGC 2 cut(s) 79, 346
Csp6I GTAC 3 cut(s) 139, 436, 754
CspI CGGWCCG 1 cut(s) 234
CviAII CATG 6 cut(s) 16, 148, 154, 160, 332, 580
CviQI GTAC 3 cut(s) 139, 436, 754
DdeI CTNAG 1 cut(s) 671
DpnI GATC 3 cut(s) 344, 353, 828
DpnII GATC 3 cut(s) 342, 351, 826
EaeI YGGCCR 4 cut(s) 3, 36, 238, 549
EagI CGGCCG 1 cut(s) 549
Eam1104I CTCTTC 4 cut(s) 222, 445, 550, 674
EarI CTCTTC 4 cut(s) 222, 445, 550, 674
EciI GGCGGA 1 cut(s) 348
EclXI CGGCCG 1 cut(s) 549
Eco130I CCWWGG 3 cut(s) 147, 606, 788
Eco24I GRGCYC 2 cut(s) 262, 614
Eco31I GGTCTC 1 cut(s) 547
Eco47I GGWCC 2 cut(s) 228, 234
Eco52I CGGCCG 1 cut(s) 549
EcoRII CCWGG 3 cut(s) 63, 378, 537
EcoT14I CCWWGG 3 cut(s) 147, 606, 788
EcoT38I GRGCYC 2 cut(s) 262, 614
ErhI CCWWGG 3 cut(s) 147, 606, 788
FaeI CATG 6 cut(s) 19, 151, 157, 163, 335, 583
FaqI GGGAC 4 cut(s) 412, 494, 520, 846
FatI CATG 6 cut(s) 15, 147, 153, 159, 331, 579
FauI CCCGC 2 cut(s) 254, 380
FblI GTMKAC 2 cut(s) 317, 327
Fnu4HI GCNGC 8 cut(s) 72, 385, 447, 497, 560, 563, 595, 646
FokI GGATG 3 cut(s) 403, 746, 771
FriOI GRGCYC 2 cut(s) 262, 614
Fsp4HI GCNGC 8 cut(s) 72, 385, 447, 497, 560, 563, 595, 646
FspBI CTAG 2 cut(s) 44, 321
GluI GCNGC 8 cut(s) 72, 385, 447, 497, 560, 563, 595, 646
GsaI CCCAGC 1 cut(s) 124
HaeIII GGCC 6 cut(s) 5, 38, 81, 225, 240, 551
HgaI GACGC 2 cut(s) 79, 346
Hin1I GRCGYC 2 cut(s) 183, 338
Hin1II CATG 6 cut(s) 19, 151, 157, 163, 335, 583
HincII GTYRAC 2 cut(s) 318, 474
HindII GTYRAC 2 cut(s) 318, 474
HindIII AAGCTT 1 cut(s) 706
HinfI GANTC 4 cut(s) 290, 308, 407, 482
Hpy166II GTNNAC 4 cut(s) 318, 328, 414, 474
Hpy188I TCNGA 3 cut(s) 107, 487, 601
Hpy188III TCNNGA 3 cut(s) 94, 202, 253
Hpy8I GTNNAC 4 cut(s) 318, 328, 414, 474
Hpy99I CGWCG 9 cut(s) 62, 95, 179, 185, 188, 319, 340, 454, 472
HpyAV CCTTC 1 cut(s) 280
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 4 cut(s) 168, 373, 594, 629
HpyF10VI GCNNNNNNNGC 6 cut(s) 87, 185, 246, 266, 346, 651
HpyF3I CTNAG 1 cut(s) 671
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 2 cut(s) 183, 338
Hsp92II CATG 6 cut(s) 19, 151, 157, 163, 335, 583
Kpn2I TCCGGA 1 cut(s) 252
Kzo9I GATC 3 cut(s) 342, 351, 826
LmnI GCTCC 4 cut(s) 361, 602, 617, 638
Lsp1109I GCAGC 4 cut(s) 433, 508, 606, 632
MaeI CTAG 2 cut(s) 44, 321
MaeII ACGT 1 cut(s) 183
MalI GATC 3 cut(s) 344, 353, 828
MboI GATC 3 cut(s) 342, 351, 826
MboII GAAGA 8 cut(s) 59, 209, 303, 432, 501, 537, 561, 691
MhlI GDGCHC 2 cut(s) 262, 614
MluCI AATT 6 cut(s) 28, 424, 522, 798, 839, 871
MlyI GAGTC 3 cut(s) 284, 401, 476
MmeI TCCRAC 2 cut(s) 510, 624
MnlI CCTC 7 cut(s) 85, 127, 273, 291, 388, 646, 675
MroI TCCGGA 1 cut(s) 252
MseI TTAA 2 cut(s) 710, 870
MslI CAYNNNNRTG 2 cut(s) 152, 158
MspR9I CCNGG 4 cut(s) 65, 232, 380, 539
MvaI CCWGG 3 cut(s) 65, 380, 539
MvnI CGCG 1 cut(s) 515
MwoI GCNNNNNNNGC 6 cut(s) 87, 185, 246, 266, 346, 651
NciI CCSGG 1 cut(s) 232
NcoI CCATGG 1 cut(s) 147
NdeII GATC 3 cut(s) 342, 351, 826
NlaIII CATG 6 cut(s) 19, 151, 157, 163, 335, 583
NlaIV GGNNCC 2 cut(s) 230, 613
NmeAIII GCCGAG 1 cut(s) 14
PcsI WCGNNNNNNNCGW 2 cut(s) 464, 512
PfeI GAWTC 1 cut(s) 308
PflFI GACNNNGTC 1 cut(s) 293
PflMI CCANNNNNTGG 1 cut(s) 148
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 8 cut(s) 73, 386, 448, 498, 561, 564, 596, 647
PleI GAGTC 3 cut(s) 284, 401, 476
PpsI GAGTC 3 cut(s) 284, 401, 476
PshAI GACNNNNGTC 1 cut(s) 323
Psp6I CCWGG 3 cut(s) 63, 378, 537
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 3 cut(s) 63, 378, 537
PspN4I GGNNCC 2 cut(s) 230, 613
PspPI GGNCC 3 cut(s) 223, 228, 234
PsrI GAACNNNNNNTAC 2 cut(s) 678, 710
PstNI CAGNNNCTG 1 cut(s) 502
PsyI GACNNNGTC 1 cut(s) 293
RsaI GTAC 3 cut(s) 140, 437, 755
RsaNI GTAC 3 cut(s) 139, 436, 754
RseI CAYNNNNRTG 2 cut(s) 152, 158
Rsr2I CGGWCCG 1 cut(s) 234
RsrII CGGWCCG 1 cut(s) 234
SalI GTCGAC 1 cut(s) 316
SaqAI TTAA 2 cut(s) 710, 870
SatI GCNGC 8 cut(s) 72, 385, 447, 497, 560, 563, 595, 646
Sau3AI GATC 3 cut(s) 342, 351, 826
Sau96I GGNCC 3 cut(s) 223, 228, 234
SchI GAGTC 3 cut(s) 284, 401, 476
ScrFI CCNGG 4 cut(s) 65, 232, 380, 539
SduI GDGCHC 2 cut(s) 262, 614
SgrAI CRCCGGYG 1 cut(s) 361
SinI GGWCC 2 cut(s) 228, 234
SmiMI CAYNNNNRTG 2 cut(s) 152, 158
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 6 cut(s) 28, 424, 522, 798, 839, 871
SspMI CTAG 2 cut(s) 44, 321
StyD4I CCNGG 4 cut(s) 63, 230, 378, 537
StyI CCWWGG 3 cut(s) 147, 606, 788
TaiI ACGT 1 cut(s) 186
TaqI TCGA 4 cut(s) 93, 317, 812, 822
TasI AATT 6 cut(s) 28, 424, 522, 798, 839, 871
TauI GCSGC 4 cut(s) 74, 387, 562, 565
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 710, 870
Tru9I TTAA 2 cut(s) 710, 870
TseI GCWGC 4 cut(s) 446, 496, 594, 645
TspDTI ATGAA 2 cut(s) 4, 596
TspGWI ACGGA 2 cut(s) 283, 771
Tth111I GACNNNGTC 1 cut(s) 293
Van91I CCANNNNNTGG 1 cut(s) 148
VpaK11BI GGWCC 2 cut(s) 228, 234
XapI RAATTY 3 cut(s) 28, 424, 798
XmiI GTMKAC 2 cut(s) 317, 327
XspI CTAG 2 cut(s) 44, 321
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.