MD02G1197400.v1.1
MADS Family

MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006997
Physical Location & Seq
Forward (+)
19095306 .. 19100985
5680 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1197400.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGTGAGAGGGAAAACTCAGATGAGGCGCATAGAGAACACGACGAGTCGTCAGGTGACATTCTCAAAGAGGAGAAGTGGTCTGCTAAAGAAGGCCTTTGAGCTCTCAGTTCTTTGCGATGCTGAGGTTTCCCTCATCATCTTCTCTCCCAGAGGAAAACTCTACGAATTTGCAAGTTCCAGCATGCAGGGAACCATAGAGCGTTATCAGAAGCATGCGAAAGACAATCAAACCAACGACAAATCCAGTTCCAGTGAACAAAATCATCTGAAGCAAGAAGCAACTAGCATGATGAAGCAGATAGAGCTTCTTGAAGTATCAAAACGGAAACTCTTGGGAGAGGGTCTAGGATCATGCACTCTTGCAGAATTACAAGAAATAGAAGACCAGTTGGAGAAGAGCGTCTACAACGTCCGAGCCCGAAAGAGTCAGGTTTTCAAGGAACAGATTGAGCAACTGAGAGAAAAGGAAAAACTCCTCACAGCTGAAAATACAAGACTGGTTGAGAAGTATGGTAGTTTCAAGAAAACATTGCACGAGCGAAGAGAAAAGACACCTTACAATGAAAGTAGTACAAGCTCGGATGTTGAGACTGAATTGTTCATTGGACTGCCGGAAAGTAGGGCGAGGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000060 GO:0001101 GO:0003006 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0007275 GO:0008104 GO:0008134 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009739 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009909 GO:0009911 GO:0010033 GO:0010073 GO:0010074 GO:0010077 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015031 GO:0015833 GO:0017038 GO:0019219 GO:0019222 GO:0019827 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0033993 GO:0034504 GO:0034613 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045184 GO:0045893 GO:0045935 GO:0046907 GO:0048367 GO:0048507 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0060255 GO:0061458 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0080090 GO:0090567 GO:0098727 GO:0140110 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04013 ko04022 ko04371 ko05418 map04013 map04022 map04371 map05418
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

212

Amino Acids

24.37

Weight (kDa)

9.43

Isoelectric Point (pI)

57.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 2.4e-26 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 82 - 170 8.2e-26 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 405
AclWI GGATC 1 cut(s) 358
AcsI RAATTY 1 cut(s) 167
AcuI CTGAAG 1 cut(s) 290
AfaI GTAC 1 cut(s) 574
AgsI TTSAA 3 cut(s) 314, 439, 523
AhdI GACNNNNNGTC 1 cut(s) 48
AjuI GAANNNNNNNTTGG 2 cut(s) 588, 620
AluBI AGCT 4 cut(s) 103, 307, 485, 579
AluI AGCT 4 cut(s) 103, 307, 485, 579
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 584
AlwI GGATC 1 cut(s) 358
AoxI GGCC 1 cut(s) 93
ApoI RAATTY 1 cut(s) 167
AspLEI GCGC 2 cut(s) 30, 633
AsuHPI GGTGA 2 cut(s) 16, 67
BanII GRGCYC 2 cut(s) 105, 421
BarI GAAGNNNNNNTAC 2 cut(s) 389, 421
BauI CACGAG 1 cut(s) 536
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 105
BbvCI CCTCAGC 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 584
BfaI CTAG 3 cut(s) 285, 347, 634
BfoI RGCGCY 1 cut(s) 634
BmeRI GACNNNNNGTC 1 cut(s) 48
BmiI GGNNCC 1 cut(s) 193
BmsI GCATC 1 cut(s) 109
BpiI GAAGAC 1 cut(s) 390
BplI GAGNNNNNCTC 2 cut(s) 144, 176
Bpu10I CCTNAGC 1 cut(s) 123
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bse1I ACTGG 4 cut(s) 246, 252, 388, 504
Bse3DI GCAATG 1 cut(s) 530
BseGI GGATG 1 cut(s) 589
BseMI GCAATG 1 cut(s) 530
BseMII CTCAG 4 cut(s) 32, 114, 120, 449
BseNI ACTGG 4 cut(s) 246, 252, 388, 504
BseRI GAGGAG 2 cut(s) 85, 467
BshFI GGCC 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 105
BsiSI CCGG 1 cut(s) 614
BsmAI GTCTC 1 cut(s) 584
BsnI GGCC 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 105, 421
Bsp143I GATC 1 cut(s) 350
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 4 cut(s) 31, 115, 119, 450
BspLI GGNNCC 1 cut(s) 193
BspPI GGATC 1 cut(s) 358
BspQI GCTCTTC 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 530
BsrI ACTGG 4 cut(s) 246, 252, 388, 504
BssMI GATC 1 cut(s) 350
BssSI CACGAG 1 cut(s) 536
Bst2BI CACGAG 1 cut(s) 536
Bst6I CTCTTC 2 cut(s) 392, 538
BstC8I GCNNGC 2 cut(s) 185, 216
BstDEI CTNAG 4 cut(s) 18, 106, 123, 458
BstF5I GGATG 1 cut(s) 589
BstH2I RGCGCY 1 cut(s) 634
BstHHI GCGC 2 cut(s) 30, 633
BstKTI GATC 1 cut(s) 353
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 1 cut(s) 350
BstMWI GCNNNNNNNGC 1 cut(s) 304
BstNSI RCATGY 2 cut(s) 187, 218
BstV2I GAAGAC 1 cut(s) 390
BsuRI GGCC 1 cut(s) 95
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 1 cut(s) 589
BtsIMutI CAGTG 1 cut(s) 259
Cac8I GCNNGC 2 cut(s) 185, 216
CfoI GCGC 2 cut(s) 30, 633
CseI GACGC 1 cut(s) 391
Csp6I GTAC 1 cut(s) 573
CviAII CATG 4 cut(s) 184, 215, 289, 354
CviJI RGCY 6 cut(s) 95, 103, 307, 419, 485, 579
CviKI_1 RGCY 6 cut(s) 95, 103, 307, 419, 485, 579
CviQI GTAC 1 cut(s) 573
DdeI CTNAG 4 cut(s) 18, 106, 123, 458
DpnI GATC 1 cut(s) 352
DpnII GATC 1 cut(s) 350
DriI GACNNNNNGTC 1 cut(s) 48
Eam1104I CTCTTC 2 cut(s) 392, 538
Eam1105I GACNNNNNGTC 1 cut(s) 48
EarI CTCTTC 2 cut(s) 392, 538
Ecl136II GAGCTC 1 cut(s) 103
Eco147I AGGCCT 1 cut(s) 95
Eco24I GRGCYC 2 cut(s) 105, 421
Eco53kI GAGCTC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 103
EcoT38I GRGCYC 2 cut(s) 105, 421
FaeI CATG 4 cut(s) 187, 218, 292, 357
FaiI YATR 7 cut(s) 32, 185, 197, 216, 290, 355, 513
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FatI CATG 4 cut(s) 183, 214, 288, 353
FblI GTMKAC 1 cut(s) 405
FokI GGATG 1 cut(s) 596
FriOI GRGCYC 2 cut(s) 105, 421
FspBI CTAG 3 cut(s) 285, 347, 634
GlaI GCGC 2 cut(s) 29, 632
HaeII RGCGCY 1 cut(s) 634
HaeIII GGCC 1 cut(s) 95
HapII CCGG 1 cut(s) 614
HgaI GACGC 1 cut(s) 391
HhaI GCGC 2 cut(s) 30, 633
Hin1II CATG 4 cut(s) 187, 218, 292, 357
Hin6I GCGC 2 cut(s) 28, 631
HinP1I GCGC 2 cut(s) 28, 631
HinfI GANTC 2 cut(s) 46, 427
HpaII CCGG 1 cut(s) 614
HphI GGTGA 2 cut(s) 16, 67
Hpy166II GTNNAC 2 cut(s) 257, 406
Hpy188I TCNGA 5 cut(s) 21, 210, 270, 416, 583
Hpy188III TCNNGA 2 cut(s) 311, 523
Hpy8I GTNNAC 2 cut(s) 257, 406
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 1 cut(s) 85
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 5 cut(s) 173, 187, 357, 365, 535
HpyF10VI GCNNNNNNNGC 1 cut(s) 304
HpyF3I CTNAG 4 cut(s) 18, 106, 123, 458
HpySE526I ACGT 1 cut(s) 411
Hsp92II CATG 4 cut(s) 187, 218, 292, 357
HspAI GCGC 2 cut(s) 28, 631
Kzo9I GATC 1 cut(s) 350
LguI GCTCTTC 1 cut(s) 392
LweI GCATC 1 cut(s) 109
MaeI CTAG 3 cut(s) 285, 347, 634
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 1 cut(s) 55
MalI GATC 1 cut(s) 352
MboI GATC 1 cut(s) 350
MboII GAAGA 4 cut(s) 133, 395, 409, 555
MhlI GDGCHC 2 cut(s) 105, 421
MluCI AATT 3 cut(s) 167, 368, 596
MlyI GAGTC 2 cut(s) 55, 436
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 8 cut(s) 18, 63, 118, 143, 146, 334, 488, 621
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 1 cut(s) 614
MwoI GCNNNNNNNGC 1 cut(s) 304
NdeII GATC 1 cut(s) 350
NlaIII CATG 4 cut(s) 187, 218, 292, 357
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 1 cut(s) 55
NspI RCATGY 2 cut(s) 187, 218
PaeI GCATGC 2 cut(s) 187, 218
PceI AGGCCT 1 cut(s) 95
PciSI GCTCTTC 1 cut(s) 392
PleI GAGTC 2 cut(s) 54, 435
PpsI GAGTC 2 cut(s) 54, 435
Psp124BI GAGCTC 1 cut(s) 105
PspN4I GGNNCC 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 1 cut(s) 574
RsaNI GTAC 1 cut(s) 573
SacI GAGCTC 1 cut(s) 105
SapI GCTCTTC 1 cut(s) 392
Sau3AI GATC 1 cut(s) 350
SchI GAGTC 2 cut(s) 55, 436
SduI GDGCHC 2 cut(s) 105, 421
SetI ASST 9 cut(s) 57, 105, 129, 309, 414, 435, 487, 559, 581
SfaNI GCATC 1 cut(s) 109
SphI GCATGC 2 cut(s) 187, 218
Sse9I AATT 3 cut(s) 167, 368, 596
SseBI AGGCCT 1 cut(s) 95
SspMI CTAG 3 cut(s) 285, 347, 634
SstI GAGCTC 1 cut(s) 105
StuI AGGCCT 1 cut(s) 95
TaiI ACGT 1 cut(s) 414
TasI AATT 3 cut(s) 167, 368, 596
TatI WGTACW 1 cut(s) 572
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 1 cut(s) 55
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 3 cut(s) 308, 579, 592
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 1 cut(s) 259
XapI RAATTY 1 cut(s) 167
XceI RCATGY 2 cut(s) 187, 218
XmiI GTMKAC 1 cut(s) 405
XspI CTAG 3 cut(s) 285, 347, 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.