Rroxscaffold_4G00303610
MADS Family

MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006997
Physical Location & Seq
Forward (+)
23986448 .. 23993941
7494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303610.1

Sequence Viewer

Length: 648 bp
ATGGTGCGAGGCAAAACCCAGATGAGGCGGATAGAGAACGCCACGAGCCGTCAAGTCACCTTCTCGAAGCGGCGAAGCGGTCTTCTGAAGAAGGCTTTCGAGCTCTCCATTCTCTGCGATGCTGAGGTTGCGCTCATAATCTTCTCTCCGAGAGGAAAGCTCTACGAATTCGCAAGCTCCAGCATGCAGACAACTATAGAACGTTATGAGAAGCATACAAAAGACAACCAAGCCAACAACAAATCTGTTGCCAGTGAACAAAATGTGCAGCAGCTCAAGCATGAAGCAACTAGCATGATGAAGCAGATAGAGCATCTTGAAGTTTCGAAACGGAAACTATTGGGAGAGAGTCTAGGACTATGCAGTATTGACGAACTGCAAGAAATAGAGCAACAGTTGGAGAGGAGTGTGAACAGCATTCGAGCAAGGAAGGCACAGGTTTTCAAGGAACAGATTGAGCAACTGAAAGAAAAGGAAAGAGTCCTCACAGCTGAAAATCAGAGGCTAAGTGAGAAGTGTGAGGCTATGCAACCGATGCAACCAGTAAGTGAGCAGAGAGAAAACTTAGCCTGCCCTGAAAGTAGTCCAAGTTCAGATGTTGAGACTGAATTGTTCATTGGACTGCCAGAAAGAAGATCGAAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000060 GO:0001101 GO:0003006 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0007275 GO:0008104 GO:0008134 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009739 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009909 GO:0009911 GO:0010033 GO:0010073 GO:0010074 GO:0010077 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015031 GO:0015833 GO:0017038 GO:0019219 GO:0019222 GO:0019827 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0033993 GO:0034504 GO:0034613 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045184 GO:0045893 GO:0045935 GO:0046907 GO:0048367 GO:0048507 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0060255 GO:0061458 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0080090 GO:0090567 GO:0098727 GO:0140110 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04013 ko04022 ko04371 ko05418 map04013 map04022 map04371 map05418
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

215

Amino Acids

24.72

Weight (kDa)

8.63

Isoelectric Point (pI)

64.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 6.4e-27 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 85 - 173 1.3e-25 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 28, 70, 78
AclI AACGTT 1 cut(s) 202
AcsI RAATTY 1 cut(s) 167
AcuI CTGAAG 1 cut(s) 107
AfiI CCNNNNNNNGG 1 cut(s) 24
AgsI TTSAA 2 cut(s) 320, 445
AjuI GAANNNNNNNTTGG 2 cut(s) 600, 632
AluBI AGCT 5 cut(s) 103, 160, 177, 274, 491
AluI AGCT 5 cut(s) 103, 160, 177, 274, 491
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 596
ApeKI GCWGC 2 cut(s) 268, 271
ApoI RAATTY 1 cut(s) 167
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 49
AsuII TTCGAA 1 cut(s) 326
BanII GRGCYC 1 cut(s) 105
BauI CACGAG 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 105
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 280, 283
BceAI ACGGC 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 596
BfaI CTAG 3 cut(s) 291, 353, 646
BfmI CTRYAG 1 cut(s) 195
BisI GCNGC 3 cut(s) 71, 269, 272
BlsI GCNGC 3 cut(s) 72, 270, 273
BmsI GCATC 3 cut(s) 109, 322, 525
BpiI GAAGAC 1 cut(s) 74
BplI GAGNNNNNCTC 2 cut(s) 144, 176
BpmI CTGGAG 1 cut(s) 163
Bpu10I CCTNAGC 1 cut(s) 123
Bpu14I TTCGAA 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 24
Bse1I ACTGG 2 cut(s) 252, 542
BseLI CCNNNNNNNGG 1 cut(s) 24
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 2 cut(s) 252, 542
BseRI GAGGAG 1 cut(s) 418
BseXI GCAGC 2 cut(s) 280, 283
BsgI GTGCAG 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 24
BsmAI GTCTC 1 cut(s) 596
BsmI GAATGC 1 cut(s) 417
Bsp119I TTCGAA 1 cut(s) 326
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 1 cut(s) 635
BspACI CCGC 3 cut(s) 28, 70, 78
BspCNI CTCAG 1 cut(s) 115
BspT104I TTCGAA 1 cut(s) 326
BsrI ACTGG 2 cut(s) 252, 542
BssMI GATC 1 cut(s) 635
BssSI CACGAG 1 cut(s) 43
Bst2BI CACGAG 1 cut(s) 43
Bst4CI ACNGT 1 cut(s) 396
BstAPI GCANNNNNTGC 1 cut(s) 535
BstBI TTCGAA 1 cut(s) 326
BstC8I GCNNGC 3 cut(s) 175, 185, 571
BstDEI CTNAG 3 cut(s) 123, 506, 565
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 1 cut(s) 638
BstMAI GTCTC 1 cut(s) 596
BstMBI GATC 1 cut(s) 635
BstMWI GCNNNNNNNGC 5 cut(s) 128, 277, 310, 431, 535
BstNSI RCATGY 1 cut(s) 187
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 280, 283
BstV2I GAAGAC 1 cut(s) 74
BtgZI GCGATG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 259
Cac8I GCNNGC 3 cut(s) 175, 185, 571
CfoI GCGC 1 cut(s) 133
CviAII CATG 3 cut(s) 184, 281, 295
DdeI CTNAG 3 cut(s) 123, 506, 565
DpnI GATC 1 cut(s) 637
DpnII GATC 1 cut(s) 635
EciI GGCGGA 1 cut(s) 43
Ecl136II GAGCTC 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 103
EcoRI GAATTC 1 cut(s) 167
EcoT38I GRGCYC 1 cut(s) 105
FaeI CATG 3 cut(s) 187, 284, 298
FaiI YATR 9 cut(s) 137, 185, 197, 207, 216, 282, 296, 361, 527
FatI CATG 3 cut(s) 183, 280, 294
Fnu4HI GCNGC 3 cut(s) 71, 269, 272
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 3 cut(s) 71, 269, 272
FspBI CTAG 3 cut(s) 291, 353, 646
GlaI GCGC 1 cut(s) 132
GluI GCNGC 3 cut(s) 71, 269, 272
GsuI CTGGAG 1 cut(s) 163
HhaI GCGC 1 cut(s) 133
Hin1II CATG 3 cut(s) 187, 284, 298
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HinfI GANTC 2 cut(s) 349, 480
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 2 cut(s) 257, 412
Hpy188I TCNGA 4 cut(s) 87, 150, 501, 595
Hpy188III TCNNGA 2 cut(s) 64, 317
Hpy8I GTNNAC 2 cut(s) 257, 412
HpyAV CCTTC 3 cut(s) 70, 85, 424
HpyCH4III ACNGT 1 cut(s) 396
HpyCH4IV ACGT 1 cut(s) 202
HpyCH4V TGCA 6 cut(s) 187, 268, 363, 379, 529, 538
HpyF10VI GCNNNNNNNGC 5 cut(s) 128, 277, 310, 431, 535
HpyF3I CTNAG 3 cut(s) 123, 506, 565
HpySE526I ACGT 1 cut(s) 202
Hsp92II CATG 3 cut(s) 187, 284, 298
HspAI GCGC 1 cut(s) 131
Kzo9I GATC 1 cut(s) 635
LmnI GCTCC 1 cut(s) 182
LpnPI CCDG 8 cut(s) 32, 193, 265, 422, 555, 583, 588, 639
Lsp1109I GCAGC 2 cut(s) 280, 283
LweI GCATC 3 cut(s) 109, 322, 525
MaeI CTAG 3 cut(s) 291, 353, 646
MaeII ACGT 1 cut(s) 202
MaeIII GTNAC 1 cut(s) 55
MalI GATC 1 cut(s) 637
MboI GATC 1 cut(s) 635
MboII GAAGA 4 cut(s) 74, 100, 133, 645
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 2 cut(s) 167, 608
MlyI GAGTC 2 cut(s) 358, 489
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 7 cut(s) 18, 118, 146, 396, 494, 495, 514
MroXI GAANNNNTTC 1 cut(s) 95
MspA1I CMGCKG 1 cut(s) 491
Mva1269I GAATGC 1 cut(s) 417
MwoI GCNNNNNNNGC 5 cut(s) 128, 277, 310, 431, 535
NdeII GATC 1 cut(s) 635
NlaIII CATG 3 cut(s) 187, 284, 298
NmuCI GTSAC 1 cut(s) 55
NspI RCATGY 1 cut(s) 187
NspV TTCGAA 1 cut(s) 326
PaeI GCATGC 1 cut(s) 187
PctI GAATGC 1 cut(s) 417
PdmI GAANNNNTTC 1 cut(s) 95
PkrI GCNGC 3 cut(s) 72, 270, 273
PleI GAGTC 2 cut(s) 357, 488
PpsI GAGTC 2 cut(s) 357, 488
Psp124BI GAGCTC 1 cut(s) 105
Psp1406I AACGTT 1 cut(s) 202
PsrI GAACNNNNNNTAC 2 cut(s) 574, 606
PvuII CAGCTG 1 cut(s) 491
SacI GAGCTC 1 cut(s) 105
SatI GCNGC 3 cut(s) 71, 269, 272
Sau3AI GATC 1 cut(s) 635
SchI GAGTC 2 cut(s) 358, 489
SduI GDGCHC 1 cut(s) 105
SetI ASST 9 cut(s) 62, 105, 129, 162, 179, 205, 276, 441, 493
SfaNI GCATC 3 cut(s) 109, 322, 525
SfcI CTRYAG 1 cut(s) 195
SfuI TTCGAA 1 cut(s) 326
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SphI GCATGC 1 cut(s) 187
Sse9I AATT 2 cut(s) 167, 608
SsiI CCGC 3 cut(s) 28, 70, 78
SspMI CTAG 3 cut(s) 291, 353, 646
SstI GAGCTC 1 cut(s) 105
TaaI ACNGT 1 cut(s) 396
TaiI ACGT 1 cut(s) 205
TaqI TCGA 5 cut(s) 65, 99, 326, 421, 638
TasI AATT 2 cut(s) 167, 608
TauI GCSGC 1 cut(s) 73
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 2 cut(s) 268, 271
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 3 cut(s) 297, 314, 604
TspGWI ACGGA 1 cut(s) 346
TspRI CASTG 1 cut(s) 259
XapI RAATTY 1 cut(s) 167
XceI RCATGY 1 cut(s) 187
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 3 cut(s) 291, 353, 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.