MD02G1235200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012289
Physical Location & Seq
Forward (+)
28145415 .. 28147718
2304 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1235200.v1.1.491

Sequence Viewer

Length: 2259 bp
ATGAAAACTGGAAACTTGAGCAGTACAAGTTGCCGTACCAAAGAAGTTGTGTATCCGTTATTCTCAAAAGCTCTTTCCTCTGCTAAAAACTGCACTGAATTACACAAAGTCCACTCACAGATTGTCACTTCAGGATTACACCATTCCGTCTTCTTCTCCGGCAAACTCATAAGCAAATATGCTCAGCTCAGAGACCCCATTTCCTCCCTCTCAGTTTTCCACCAAGCTCTTCCCAAAAGCAATGCCTACCTATGGAATTCAATCATCAGAGCGCTTATCCACAATGGGTTTTACTCAAAAGCTCTTGACCACTACACTGAAATGGATAAATTGAATGTTCGACCTGATAGGTATACATTTCCTTCAGTGATCAATGCCTGTGCTGCGTTATGTGATCTGGAAATGGGCATGATTGTTCATCAGCGTGTTTCCGAAATGAGTTTTGGATCAGACTTGTACATTGGTAATGCAGTGATAGACATGTATTGTAGATTTGGTGAACTTGGCAAAGCACGGCAACTGTTTGAGCAAATGCCTCAAAGGGATATTGTGTCGTGGAATACTGTGATTTCGGGCTACAGTTCGAATGGATATTGGGAGGGAGCTTTGGAAAATTTTCGTAGGCTGAGAATGGACGGGTTGTTACCAGATTCTTTTTCCATATCTGGTATAGTTCATGCTTTGGTTGAGAAGATTGGGGTCCATGCTGATGTTTTAGTAAGCAATGGATTTCTTTCAATGTACTTCAAATTTGGTTGGTTAAAAGATGCTCAATGTCTTTTTGAGAAGATGGTTGTTAGGGACTCGGTTAGTTGGAACACTGTGATTTGTGGGTACTCTCAGTTGGGGTTGTTTAAAGACTCAATAAATTTGTTTAGGCAAATGGTGAATGAATTTACACCTGATTTGCTAACAATCACTTCTGTTCTCCGTGCTTGTGCTCATTTACAGGATCTGGGACTTGCAAGATATGTTCATGACTACATGAAAAGAAGTGCTTTTCAATTTGATACTATGGCAAATAACATACTTATTGATATGTATGCAAAATGTGGAAACTTATTAGCTTCACAAGAAGTATTTGATCATATGGAATGTAGGGATGCTGTGTCATGGAATTCATTGATCAATAGTTATTTTCTTAATGGTTGTTCTGATGAAGGGTTCAACCTTTTTAAGGCGATGAAGAGTAGCGTGAAACCTGACTCTGTGAGTTATGTGATGATACTGTCAAATTCTACCCAGCTAGCAGATGTGGACAAGGGGAAAATGATCCACTGTGATACGGTAAAATTGGGATTCAATTTAGATGTTATCGTCAACAATGCTCTTGTAGATATATATGCTAAATGTGGCAAAATAAAGGATTCGCTACTAGTATTTGAGAATATGGAAGTTCGTGATGTAGTAACATGGAATACCGTAATTGCTGCTTGCATTCATTATGACGATTGTAGTTTAGGGTTAAGAATGATTCGTAGAATGAGGAATGAGGGAGTGATGCCCGATGTGGCCACCATGTTAGGTATCTTGCCTGTGTGTTCCTTGTTTGCTGCAAAGCAACAAGGAAAAGAGATCCATGGGTGCATTTTAAGGTTAGGATTTCATTCAGATGTCCCTGTTGGGAATGCACTAATAGAAATGTATTCTAGCTGTGGTAGTTTGGAAAACTCCATACGAGTATTTGAGCATATGAGAAAGAAGGATGTGGTGACATGGACCTCCATGATCTCTGCCTATGGGACATATGGTGACGGAGTGAAAGCAGTAAGAACTTTTGCAGAAATGGAAGCAACAGGTGTCCTTCCTGATCATCTTGCTTTCCTTGCTGTCATATTTTCTTGTAGCCATTCAGGTTTAGTAGAAGAAGGTATGGCTTACTTCAACCGGATGAAGAACTGCAAACTTGAACCTAGGATCGAACACTTTGCTTGTGTAGTTGACCTCCTCTCACGGTGTGGCTTATTAGCTCAAGCAGAGGACTTTATCCATTCAATGCCAATGAAGGCAGATGCGAGTATATGGGGATCTTTACTTAGTGCTTGTCGAGCTAGTGGAGATACAGATATAGCTGCACGTGTCTCAGAACAGATAATTCAATTGAATTCTAATGATACAGGGTATCATGTTTTGGTCTCAAATGTATATGCAGCTTTAGGGAAGTGGGATCAAGTGAGAAGAATTCGGAAATGCATGCAAGCTAAAGGACTCACAAAAGATCCTGGATTTAGCTGGATGGAGATTCAAAAGAAAGTCTGA

Protein Analysis

753

Amino Acids

84.25

Weight (kDa)

7.27

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 81 - 128 3e-09 PPR repeat family
PPR_2 PF13041 181 - 229 3.3e-08 PPR repeat family
PPR_2 PF13041 268 - 315 7.1e-08 PPR repeat family
PPR_2 PF13041 368 - 410 9.9e-08 PPR repeat family
PPR PF01535 370 - 397 6.2e-06 PPR repeat
PPR_2 PF13041 467 - 507 6.4e-06 PPR repeat family
PPR_2 PF13041 568 - 613 1.4e-07 PPR repeat family
E_motif PF20431 688 - 747 2.5e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012289)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03580
fragaria_vesca FvH4_7g07570
malus_domestica MD02G1235200.v1.1 MD02G1235800.v1.1
prunus_persica Prupe.2G102800_v2.0.a1 Prupe.2G102800_v2.0.a1
pyrus_communis pycom02g20250
rosa_chinensis RchiOBHm_Chr1g0339001 RchiOBHm_Chr1g0343101
rosa_laevigata RLG00000029204 RLG00000029208
rosa_multiflora Rmu_sc0017585.1_g000006
rosa_roxburghii Rroxscaffold_4G00313330
rosa_rugosa Rorug01G0144200.1
rosa_samantha Rh1AG160800 Rh1BG127800 Rh1CG150300 Rh1DG164000
rosa_wichuraiana Rw1G013420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 353
AclWI GGATC 8 cut(s) 454, 960, 1267, 1570, 1925, 2035, 2175, 2213
AcoI YGGCCR 1 cut(s) 1512
AcsI RAATTY 9 cut(s) 256, 613, 749, 868, 893, 1117, 1234, 2104, 2181
AcuI CTGAAG 2 cut(s) 114, 348
AcvI CACGTG 1 cut(s) 2078
AdeI CACNNNGTG 1 cut(s) 1958
AfaI GTAC 5 cut(s) 25, 37, 458, 743, 836
AfeI AGCGCT 1 cut(s) 273
AfiI CCNNNNNNNGG 2 cut(s) 252, 1177
AflIII ACRYGT 2 cut(s) 480, 2077
AhlI ACTAGT 1 cut(s) 1375
AjnI CCWGG 1 cut(s) 2221
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
Alw21I GWGCWC 1 cut(s) 943
Alw26I GTCTC 3 cut(s) 186, 2086, 2140
AlwI GGATC 8 cut(s) 454, 960, 1267, 1570, 1925, 2035, 2175, 2213
AlwNI CAGNNNCTG 1 cut(s) 955
Aor51HI AGCGCT 1 cut(s) 273
AoxI GGCC 1 cut(s) 1512
ApeKI GCWGC 5 cut(s) 383, 1430, 1553, 2072, 2150
ApoI RAATTY 9 cut(s) 256, 613, 749, 868, 893, 1117, 1234, 2104, 2181
Asp700I GAANNNNTTC 1 cut(s) 615
AspA2I CCTAGG 1 cut(s) 1913
AspLEI GCGC 1 cut(s) 274
AspS9I GGNCC 2 cut(s) 700, 1719
AsuHPI GGTGA 4 cut(s) 509, 898, 1723, 1763
AsuII TTCGAA 1 cut(s) 584
AsuNHI GCTAGC 1 cut(s) 1246
AvaII GGWCC 2 cut(s) 700, 1719
AvrII CCTAGG 1 cut(s) 1913
BalI TGGCCA 1 cut(s) 1514
BbrPI CACGTG 1 cut(s) 2078
BbsI GAAGAC 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 943
BbvI GCAGC 5 cut(s) 370, 1417, 1540, 2059, 2162
BccI CCATC 2 cut(s) 784, 2230
BceAI ACGGC 2 cut(s) 18, 530
BcgI CGANNNNNNTGC 2 cut(s) 1910, 1944
BciT130I CCWGG 1 cut(s) 2223
BciVI GTATCC 1 cut(s) 63
BclI TGATCA 4 cut(s) 369, 1084, 1125, 1810
BcoDI GTCTC 3 cut(s) 186, 2086, 2140
BcuI ACTAGT 1 cut(s) 1375
BfaI CTAG 5 cut(s) 1247, 1376, 1650, 1914, 2052
BfmI CTRYAG 1 cut(s) 577
BfoI RGCGCY 1 cut(s) 275
BfuI GTATCC 1 cut(s) 63
BisI GCNGC 5 cut(s) 384, 1431, 1554, 2073, 2151
BlnI CCTAGG 1 cut(s) 1913
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 5 cut(s) 385, 1432, 1555, 2074, 2152
Bme1390I CCNGG 1 cut(s) 2223
Bme18I GGWCC 2 cut(s) 700, 1719
BmgT120I GGNCC 2 cut(s) 700, 1719
BmiI GGNNCC 1 cut(s) 701
BmrFI CCNGG 1 cut(s) 2223
BmsI GCATC 4 cut(s) 757, 1093, 1491, 2002
BmtI GCTAGC 1 cut(s) 1250
BpiI GAAGAC 1 cut(s) 142
Bpu1102I GCTNAGC 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 584
BpuEI CTTGAG 2 cut(s) 37, 1956
BsaAI YACGTR 1 cut(s) 2078
BsaI GGTCTC 2 cut(s) 186, 2140
BsaJI CCNNGG 2 cut(s) 1579, 1913
BsaWI WCCGGW 1 cut(s) 1887
Bsc4I CCNNNNNNNGG 2 cut(s) 252, 1177
Bse1I ACTGG 1 cut(s) 13
Bse3DI GCAATG 2 cut(s) 247, 730
BseBI CCWGG 1 cut(s) 2223
BseDI CCNNGG 2 cut(s) 1579, 1913
BseGI GGATG 4 cut(s) 1108, 1711, 1896, 2241
BseLI CCNNNNNNNGG 2 cut(s) 252, 1177
BseMI GCAATG 2 cut(s) 247, 730
BseMII CTCAG 6 cut(s) 197, 202, 225, 617, 854, 2097
BseNI ACTGG 1 cut(s) 13
BseRI GAGGAG 1 cut(s) 1937
BseXI GCAGC 5 cut(s) 370, 1417, 1540, 2059, 2162
BseYI CCCAGC 1 cut(s) 1242
BsgI GTGCAG 2 cut(s) 76, 2058
BshFI GGCC 1 cut(s) 1514
BsiHKAI GWGCWC 1 cut(s) 943
BsiSI CCGG 2 cut(s) 159, 1888
BslFI GGGAC 4 cut(s) 815, 972, 1601, 1756
BslI CCNNNNNNNGG 2 cut(s) 252, 1177
BsmAI GTCTC 3 cut(s) 186, 2086, 2140
BsmFI GGGAC 4 cut(s) 815, 972, 1601, 1756
BsmI GAATGC 2 cut(s) 1437, 1633
BsnI GGCC 1 cut(s) 1514
Bso31I GGTCTC 2 cut(s) 186, 2140
Bsp119I TTCGAA 1 cut(s) 584
Bsp1286I GDGCHC 1 cut(s) 943
Bsp1407I TGTACA 1 cut(s) 456
Bsp1720I GCTNAGC 1 cut(s) 183
Bsp19I CCATGG 1 cut(s) 1579
BspANI GGCC 1 cut(s) 1514
BspCNI CTCAG 6 cut(s) 196, 201, 224, 618, 853, 2096
BspHI TCATGA 1 cut(s) 976
BspLI GGNNCC 1 cut(s) 701
BspOI GCTAGC 1 cut(s) 1250
BspPI GGATC 8 cut(s) 454, 960, 1267, 1570, 1925, 2035, 2175, 2213
BspQI GCTCTTC 1 cut(s) 234
BspT104I TTCGAA 1 cut(s) 584
BspTNI GGTCTC 2 cut(s) 186, 2140
BsrDI GCAATG 2 cut(s) 247, 730
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 1579, 1913
BssNAI GTATAC 1 cut(s) 354
BssT1I CCWWGG 2 cut(s) 1579, 1913
Bst1107I GTATAC 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 2223
Bst4CI ACNGT 9 cut(s) 522, 565, 581, 823, 1230, 1280, 1288, 1423, 1956
Bst6I CTCTTC 2 cut(s) 234, 1181
BstAUI TGTACA 1 cut(s) 456
BstBAI YACGTR 1 cut(s) 2078
BstBI TTCGAA 1 cut(s) 584
BstC8I GCNNGC 4 cut(s) 1248, 1435, 2195, 2199
BstDEI CTNAG 7 cut(s) 183, 188, 211, 626, 840, 2036, 2083
BstDSI CCRYGG 1 cut(s) 1579
BstENI CCTNNNNNAGG 1 cut(s) 1175
BstF5I GGATG 4 cut(s) 1108, 1711, 1896, 2241
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 1 cut(s) 274
BstMAI GTCTC 3 cut(s) 186, 2086, 2140
BstMWI GCNNNNNNNGC 3 cut(s) 383, 1826, 2048
BstNI CCWGG 1 cut(s) 2223
BstNSI RCATGY 2 cut(s) 484, 2197
BstSCI CCNGG 1 cut(s) 2221
BstSFI CTRYAG 1 cut(s) 577
BstV1I GCAGC 5 cut(s) 370, 1417, 1540, 2059, 2162
BstV2I GAAGAC 1 cut(s) 142
BstX2I RGATCY 4 cut(s) 952, 1575, 2027, 2218
BstYI RGATCY 4 cut(s) 952, 1575, 2027, 2218
BstZ17I GTATAC 1 cut(s) 354
BsuI GTATCC 1 cut(s) 63
BsuRI GGCC 1 cut(s) 1514
BtgI CCRYGG 1 cut(s) 1579
BtgZI GCGATG 1 cut(s) 1196
BtsCI GGATG 4 cut(s) 1108, 1711, 1896, 2241
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 6 cut(s) 93, 315, 372, 477, 819, 1276
Cac8I GCNNGC 4 cut(s) 1248, 1435, 2195, 2199
CaiI CAGNNNCTG 1 cut(s) 955
CciI TCATGA 1 cut(s) 976
CfoI GCGC 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 700, 1719
Csp6I GTAC 5 cut(s) 24, 36, 457, 742, 835
CviQI GTAC 5 cut(s) 24, 36, 457, 742, 835
DdeI CTNAG 7 cut(s) 183, 188, 211, 626, 840, 2036, 2083
DraI TTTAAA 1 cut(s) 856
DraIII CACNNNGTG 1 cut(s) 1958
EaeI YGGCCR 1 cut(s) 1512
Eam1104I CTCTTC 2 cut(s) 234, 1181
EarI CTCTTC 2 cut(s) 234, 1181
Eco130I CCWWGG 2 cut(s) 1579, 1913
Eco31I GGTCTC 2 cut(s) 186, 2140
Eco47I GGWCC 2 cut(s) 700, 1719
Eco47III AGCGCT 1 cut(s) 273
Eco57I CTGAAG 2 cut(s) 114, 348
Eco72I CACGTG 1 cut(s) 2078
EcoNI CCTNNNNNAGG 1 cut(s) 1175
EcoRI GAATTC 4 cut(s) 256, 1117, 2104, 2181
EcoRII CCWGG 1 cut(s) 2221
EcoT14I CCWWGG 2 cut(s) 1579, 1913
EcoT22I ATGCAT 1 cut(s) 2195
ErhI CCWWGG 2 cut(s) 1579, 1913
FalI AAGNNNNNCTT 2 cut(s) 982, 1014
FaqI GGGAC 4 cut(s) 815, 972, 1601, 1756
FauNDI CATATG 3 cut(s) 1089, 1692, 1747
FbaI TGATCA 4 cut(s) 369, 1084, 1125, 1810
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 5 cut(s) 384, 1431, 1554, 2073, 2151
FokI GGATG 4 cut(s) 1115, 1718, 1903, 2248
Fsp4HI GCNGC 5 cut(s) 384, 1431, 1554, 2073, 2151
FspBI CTAG 5 cut(s) 1247, 1376, 1650, 1914, 2052
GlaI GCGC 1 cut(s) 273
GluI GCNGC 5 cut(s) 384, 1431, 1554, 2073, 2151
GsaI CCCAGC 1 cut(s) 1246
HaeII RGCGCY 1 cut(s) 275
HaeIII GGCC 1 cut(s) 1514
HapII CCGG 2 cut(s) 159, 1888
HhaI GCGC 1 cut(s) 274
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HincII GTYRAC 2 cut(s) 1321, 1942
HindII GTYRAC 2 cut(s) 1321, 1942
HinfI GANTC 9 cut(s) 650, 803, 860, 1205, 1299, 1367, 1474, 2208, 2242
HpaII CCGG 2 cut(s) 159, 1888
HphI GGTGA 4 cut(s) 509, 898, 1723, 1763
Hpy166II GTNNAC 6 cut(s) 112, 354, 500, 1258, 1321, 1942
Hpy188I TCNGA 9 cut(s) 191, 269, 433, 451, 1156, 1612, 2086, 2187, 2258
Hpy188III TCNNGA 6 cut(s) 132, 305, 398, 977, 1400, 1808
Hpy8I GTNNAC 6 cut(s) 112, 354, 500, 1258, 1321, 1942
HpyAV CCTTC 6 cut(s) 372, 1154, 1696, 1814, 1862, 1999
HpyCH4III ACNGT 9 cut(s) 522, 565, 581, 823, 1230, 1280, 1288, 1423, 1956
HpyCH4IV ACGT 1 cut(s) 2077
HpyF10VI GCNNNNNNNGC 3 cut(s) 383, 1826, 2048
HpyF3I CTNAG 7 cut(s) 183, 188, 211, 626, 840, 2036, 2083
HpySE526I ACGT 1 cut(s) 2077
HspAI GCGC 1 cut(s) 272
Ksp22I TGATCA 4 cut(s) 369, 1084, 1125, 1810
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 1 cut(s) 602
Lsp1109I GCAGC 5 cut(s) 370, 1417, 1540, 2059, 2162
LweI GCATC 4 cut(s) 757, 1093, 1491, 2002
MaeI CTAG 5 cut(s) 1247, 1376, 1650, 1914, 2052
MaeII ACGT 1 cut(s) 2077
MaeIII GTNAC 5 cut(s) 124, 642, 1408, 1711, 1751
MboII GAAGA 9 cut(s) 142, 145, 221, 703, 799, 1198, 1877, 1906, 2190
MfeI CAATTG 1 cut(s) 2099
MflI RGATCY 4 cut(s) 952, 1575, 2027, 2218
MhlI GDGCHC 1 cut(s) 943
MlsI TGGCCA 1 cut(s) 1514
MluNI TGGCCA 1 cut(s) 1514
MlyI GAGTC 4 cut(s) 797, 854, 1199, 2202
MmeI TCCRAC 1 cut(s) 794
Mox20I TGGCCA 1 cut(s) 1514
Mph1103I ATGCAT 1 cut(s) 2195
MroXI GAANNNNTTC 1 cut(s) 615
MscI TGGCCA 1 cut(s) 1514
MseI TTAA 6 cut(s) 761, 855, 1143, 1176, 1466, 1592
MslI CAYNNNNRTG 4 cut(s) 320, 423, 708, 1611
Msp20I TGGCCA 1 cut(s) 1514
MspI CCGG 2 cut(s) 159, 1888
MspR9I CCNGG 1 cut(s) 2223
MunI CAATTG 1 cut(s) 2099
Mva1269I GAATGC 2 cut(s) 1437, 1633
MvaI CCWGG 1 cut(s) 2223
MwoI GCNNNNNNNGC 3 cut(s) 383, 1826, 2048
NcoI CCATGG 1 cut(s) 1579
NdeI CATATG 3 cut(s) 1089, 1692, 1747
NheI GCTAGC 1 cut(s) 1246
NlaIV GGNNCC 1 cut(s) 701
NmuCI GTSAC 3 cut(s) 124, 1711, 1751
NsiI ATGCAT 1 cut(s) 2195
NspI RCATGY 2 cut(s) 484, 2197
NspV TTCGAA 1 cut(s) 584
PaeI GCATGC 1 cut(s) 2197
PagI TCATGA 1 cut(s) 976
PciI ACATGT 1 cut(s) 480
PciSI GCTCTTC 1 cut(s) 234
PctI GAATGC 2 cut(s) 1437, 1633
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 5 cut(s) 650, 1299, 1367, 1474, 2242
PfoI TCCNGGA 1 cut(s) 2221
PkrI GCNGC 5 cut(s) 385, 1432, 1555, 2074, 2152
PleI GAGTC 4 cut(s) 797, 854, 1199, 2202
PmaCI CACGTG 1 cut(s) 2078
PmlI CACGTG 1 cut(s) 2078
PpsI GAGTC 4 cut(s) 797, 854, 1199, 2202
Ppu21I YACGTR 1 cut(s) 2078
PscI ACATGT 1 cut(s) 480
Psp6I CCWGG 1 cut(s) 2221
PspCI CACGTG 1 cut(s) 2078
PspFI CCCAGC 1 cut(s) 1242
PspGI CCWGG 1 cut(s) 2221
PspN4I GGNNCC 1 cut(s) 701
PspPI GGNCC 2 cut(s) 700, 1719
PstNI CAGNNNCTG 1 cut(s) 955
PsuI RGATCY 4 cut(s) 952, 1575, 2027, 2218
RsaI GTAC 5 cut(s) 25, 37, 458, 743, 836
RsaNI GTAC 5 cut(s) 24, 36, 457, 742, 835
RseI CAYNNNNRTG 4 cut(s) 320, 423, 708, 1611
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 6 cut(s) 761, 855, 1143, 1176, 1466, 1592
SatI GCNGC 5 cut(s) 384, 1431, 1554, 2073, 2151
Sau96I GGNCC 2 cut(s) 700, 1719
SchI GAGTC 4 cut(s) 797, 854, 1199, 2202
ScrFI CCNGG 1 cut(s) 2223
SduI GDGCHC 1 cut(s) 943
SfaNI GCATC 4 cut(s) 757, 1093, 1491, 2002
SfcI CTRYAG 1 cut(s) 577
SfuI TTCGAA 1 cut(s) 584
SinI GGWCC 2 cut(s) 700, 1719
SmiMI CAYNNNNRTG 4 cut(s) 320, 423, 708, 1611
SmlI CTYRAG 2 cut(s) 16, 1971
SmoI CTYRAG 2 cut(s) 16, 1971
SpeI ACTAGT 1 cut(s) 1375
SphI GCATGC 1 cut(s) 2197
SspMI CTAG 5 cut(s) 1247, 1376, 1650, 1914, 2052
StyD4I CCNGG 1 cut(s) 2221
StyI CCWWGG 2 cut(s) 1579, 1913
TaaI ACNGT 9 cut(s) 522, 565, 581, 823, 1230, 1280, 1288, 1423, 1956
TaiI ACGT 1 cut(s) 2080
TaqI TCGA 4 cut(s) 340, 584, 1920, 2047
TatI WGTACW 3 cut(s) 23, 456, 741
TfiI GAWTC 5 cut(s) 650, 1299, 1367, 1474, 2242
Tru1I TTAA 6 cut(s) 761, 855, 1143, 1176, 1466, 1592
Tru9I TTAA 6 cut(s) 761, 855, 1143, 1176, 1466, 1592
TscAI CASTG 6 cut(s) 100, 322, 372, 477, 826, 1283
TseFI GTSAC 3 cut(s) 124, 1711, 1751
TseI GCWGC 5 cut(s) 383, 1430, 1553, 2072, 2150
Tsp45I GTSAC 3 cut(s) 124, 1711, 1751
TspGWI ACGGA 4 cut(s) 45, 136, 920, 1770
TspRI CASTG 6 cut(s) 100, 322, 372, 477, 826, 1283
VpaK11BI GGWCC 2 cut(s) 700, 1719
XagI CCTNNNNNAGG 1 cut(s) 1175
XapI RAATTY 9 cut(s) 256, 613, 749, 868, 893, 1117, 1234, 2104, 2181
XceI RCATGY 2 cut(s) 484, 2197
XmaJI CCTAGG 1 cut(s) 1913
XmiI GTMKAC 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 5 cut(s) 1247, 1376, 1650, 1914, 2052
Zsp2I ATGCAT 1 cut(s) 2195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.