MD02G1235800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012289
Physical Location & Seq
Forward (+)
28210934 .. 28214448
3515 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1235800.v1.1.491

Sequence Viewer

Length: 2304 bp
ATGAAAACTGGAAACTTGAGCAGTACAAGTTGCCGTACCAAAGAAGTTGTGTATCCGTTATTCTCAAAAGCTCTTTCCTCTGCTAAAAACTGCACTGAATTACACAAAGTCCACTCACAGATTGTCACTTCAGGATTACACCATTCCGTCTTCTTCTCCGGCAAACTCATAAGCAAATATGCTCAGCTCAGAGACCCCATTTCCTCCCTCTCAGTTTTCCACCAAGCTCTTCCCAAAAGCAATGCCTACCTATGGAATTCAATCATCAGAGCGCTTATCCACAATGGGTTTTACTCAAAAGCTCTTGACCACTACACTGAAATGGATAAATTGAATGTTCGACCTGATAGGTATACATTTCCTTCAGTGATCAATGCCTGTGCTGCGTTATGTGATCTGGAAATGGGCATGATTGTTCATCAGCGTGTTTCCGAAATGGGTTTTGGATCAGACTTGTACATTGGTAATGCATTGATGGACATGTATTGTAGATTTGGTGAACTTGGCAAAGCACGGCAACTGTTTGAGCAAATGCCTGAAAGGGATATTGTGTCGTGGAATACTGTGATTTCAGGCTACAGTTCGAATGGATATTGGGAGGAAGCTTTGGAAATTTTTCGTAGGCTGAGAATGGATGGGTTGTTACCGGATTCTTTTTCCATATCTGGTGTTTTGCCTGCGTGCGGAGGCTTGGGTGATGTTGAAGAGGGTCAGATAGTTCATGCTTTGGTTGAGAAGATTGGGGTCCATGCTGATGTTTTAGTAAGCAATGGACTTCTTTCAATGTACTTCAAATTTGGTTGGTTAAAAGATGCTCAAAGTCTTTTCGAGAAGATGGTAGTTAGGGACTCGGTTAGTTGGAACACTGTGATTTGTGGGTACTCTCAGTTGGGGTTGTTTAAAGACTCAATAAATTTGTTCAAGCAAATGGTGAATGAATTTACACCTGATTTGCTAACAATCACTTCTGTTCTCCGTGCTTGTGCTCATTTACGGGATCTGAGGCTTGCAAGATATGTTCATGACTACATGAAAAGAAGTGCTTTTCAATTTGATACTATGGCAAATAACATACTTATTGATATGTATGCAAAATGTGGAAACTTATTAGCTTCACAAGAAGTATTTGATCATATGGAATGTAGGGATGCTGTGTCGTGGAATTCATTGATCAATGGTTATTTTCTTAATGGTTGTTCTGATGAAGGGTTCAACCTTTTTAAGGCAATGAAGAGTAGCATCAAACCTGACTCTGTGAGTTATGTGATGATCCTGTCAATTTCTACTCAGCTAGCAGATGTGGACAAGGGGAAAATGATCCACTGTGATACGGTAAAATTGGGATTCGATTTAGATGTTATCATCAACAATGCTCTTGTAGATATATATGCTAAATGTGGCAAAATAAAGGATTCGCTACTAGTATTTGAGAATATGGAAGTTCGTGATGTAGTAACATGGAATACCATAATTGCTGCTTGCATTCACTATGACGATTGTAGTTTAGGGTTAAAAATGATTCGTAGAATGAGGAATGAAGGAGTGATGCCCGATGCGGCCACCATGTTAGGTATCTTGCCTGTATGTTCCTTGCTTGCCGCAAAGCAACAAGGAAAAGAGATCCATGGGTGCATTTTAAGGTTAGGATTTCATTCAGATGTCCCTGTTGGGAATGCACTAATAGAAATGTATTCTAGCTGTGGTAGTTTGGAAAACTCCATACGAGTATTTGAGCATATGAGAAAGAAGGATGTGGTGACATGGACCTCCATGATCTCTGCCTATGGGACATATGGTGACGGAGTGAAAGCAGTAAGAACTTTTGCAGAAATGGAAGCAACGGGTGTCCTTCCTGATCATCTTGCTTTCCTTGCTGTCATATTTTCTTGTAGCCATTCAGGTTTAGTAGAAGAAGGTCTAGCTTACTTCAACCGGATGAAGAACTACAAACTTGAACCTAGGATCGAACACTTTGCTTGTGTAGTTGACCTCCTCTCACGGTGTGGCTTATTAGCTCAAGCAGAGGACTTTATCCATTCAATGCCAATGAAGGCAGATGCGAGTATATGGGGATCTTTACTTAGTGCTTGTCGAGCTAGTGGAGATACAGATATAACTGCACGTGTCTCAGAACAGATAATTCAATTGAATTCTAATGATATAGGGTATCATGTTTTGGTCTCAAATGTATATGCAGCTTTAGGGAAGTGGGATCAAGTGAGAAGAATCCGGAAATGCATGCAAGCCAAAGGACTCACAAAAGATCCTGGATTTAGCTGGATGGAGATTCAAAAGAATGGCTGA

Protein Analysis

768

Amino Acids

85.74

Weight (kDa)

6.54

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 81 - 128 3.1e-09 PPR repeat family
PPR_2 PF13041 181 - 228 4.6e-09 PPR repeat family
PPR PF01535 184 - 214 1.1e-08 PPR repeat
PPR_2 PF13041 283 - 330 4.6e-08 PPR repeat family
PPR_2 PF13041 383 - 425 2.5e-08 PPR repeat family
PPR PF01535 385 - 412 2e-06 PPR repeat
PPR_2 PF13041 482 - 522 3.5e-06 PPR repeat family
PPR_2 PF13041 583 - 628 1.4e-07 PPR repeat family
E_motif PF20431 709 - 762 2e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012289)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03580
fragaria_vesca FvH4_7g07570
malus_domestica MD02G1235200.v1.1 MD02G1235800.v1.1
prunus_persica Prupe.2G102800_v2.0.a1 Prupe.2G102800_v2.0.a1
pyrus_communis pycom02g20250
rosa_chinensis RchiOBHm_Chr1g0339001 RchiOBHm_Chr1g0343101
rosa_laevigata RLG00000029204 RLG00000029208
rosa_multiflora Rmu_sc0017585.1_g000006
rosa_roxburghii Rroxscaffold_4G00313330
rosa_rugosa Rorug01G0144200.1
rosa_samantha Rh1AG160800 Rh1BG127800 Rh1CG150300 Rh1DG164000
rosa_wichuraiana Rw1G013420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 353
AccIII TCCGGA 1 cut(s) 2229
AciI CCGC 3 cut(s) 684, 1556, 1599
AclWI GGATC 9 cut(s) 454, 1005, 1264, 1312, 1615, 1970, 2080, 2220, 2258
AcoI YGGCCR 1 cut(s) 1557
AcsI RAATTY 7 cut(s) 256, 612, 794, 913, 938, 1162, 2149
AcuI CTGAAG 2 cut(s) 114, 348
AcvI CACGTG 1 cut(s) 2123
AdeI CACNNNGTG 1 cut(s) 2003
AfaI GTAC 5 cut(s) 25, 37, 458, 788, 881
AfeI AGCGCT 1 cut(s) 273
AfiI CCNNNNNNNGG 3 cut(s) 252, 683, 1222
AflIII ACRYGT 2 cut(s) 480, 2122
AhlI ACTAGT 1 cut(s) 1420
AjnI CCWGG 1 cut(s) 2266
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
Alw21I GWGCWC 1 cut(s) 988
Alw26I GTCTC 3 cut(s) 186, 2131, 2185
AlwI GGATC 9 cut(s) 454, 1005, 1264, 1312, 1615, 1970, 2080, 2220, 2258
Aor13HI TCCGGA 1 cut(s) 2229
Aor51HI AGCGCT 1 cut(s) 273
AoxI GGCC 1 cut(s) 1557
ApeKI GCWGC 3 cut(s) 383, 1475, 2195
ApoI RAATTY 7 cut(s) 256, 612, 794, 913, 938, 1162, 2149
Asp700I GAANNNNTTC 1 cut(s) 615
AspA2I CCTAGG 1 cut(s) 1958
AspLEI GCGC 1 cut(s) 274
AspS9I GGNCC 2 cut(s) 745, 1764
AsuHPI GGTGA 5 cut(s) 509, 707, 943, 1768, 1808
AsuII TTCGAA 1 cut(s) 584
AsuNHI GCTAGC 1 cut(s) 1291
AvaII GGWCC 2 cut(s) 745, 1764
AvrII CCTAGG 1 cut(s) 1958
BbrPI CACGTG 1 cut(s) 2123
BbsI GAAGAC 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 988
BbvI GCAGC 3 cut(s) 370, 1462, 2207
BccI CCATC 4 cut(s) 469, 629, 829, 2275
BceAI ACGGC 2 cut(s) 18, 530
BcgI CGANNNNNNTGC 2 cut(s) 1955, 1989
BciT130I CCWGG 1 cut(s) 2268
BciVI GTATCC 1 cut(s) 63
BclI TGATCA 4 cut(s) 369, 1129, 1170, 1855
BcoDI GTCTC 3 cut(s) 186, 2131, 2185
BcuI ACTAGT 1 cut(s) 1420
BfaI CTAG 6 cut(s) 1292, 1421, 1695, 1919, 1959, 2097
BfmI CTRYAG 1 cut(s) 577
BfoI RGCGCY 1 cut(s) 275
BfuI GTATCC 1 cut(s) 63
BisI GCNGC 5 cut(s) 384, 1476, 1557, 1599, 2196
BlnI CCTAGG 1 cut(s) 1958
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 5 cut(s) 385, 1477, 1558, 1600, 2197
Bme1390I CCNGG 1 cut(s) 2268
Bme18I GGWCC 2 cut(s) 745, 1764
BmgT120I GGNCC 2 cut(s) 745, 1764
BmiI GGNNCC 1 cut(s) 746
BmrFI CCNGG 1 cut(s) 2268
BmsI GCATC 6 cut(s) 802, 1138, 1248, 1536, 1543, 2047
BmtI GCTAGC 1 cut(s) 1295
BpiI GAAGAC 1 cut(s) 142
Bpu1102I GCTNAGC 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 584
BpuEI CTTGAG 2 cut(s) 37, 2001
BsaAI YACGTR 1 cut(s) 2123
BsaI GGTCTC 2 cut(s) 186, 2185
BsaJI CCNNGG 2 cut(s) 1624, 1958
BsaWI WCCGGW 3 cut(s) 646, 1932, 2229
Bsc4I CCNNNNNNNGG 3 cut(s) 252, 683, 1222
Bse1I ACTGG 1 cut(s) 13
Bse3DI GCAATG 3 cut(s) 247, 775, 1233
BseAI TCCGGA 1 cut(s) 2229
BseBI CCWGG 1 cut(s) 2268
BseDI CCNNGG 2 cut(s) 1624, 1958
BseGI GGATG 5 cut(s) 640, 1153, 1756, 1941, 2286
BseLI CCNNNNNNNGG 3 cut(s) 252, 683, 1222
BseMI GCAATG 3 cut(s) 247, 775, 1233
BseMII CTCAG 8 cut(s) 197, 202, 225, 617, 899, 992, 1301, 2142
BseNI ACTGG 1 cut(s) 13
BseRI GAGGAG 1 cut(s) 1982
BseXI GCAGC 3 cut(s) 370, 1462, 2207
BsgI GTGCAG 2 cut(s) 76, 2103
BshFI GGCC 1 cut(s) 1559
BsiHKAI GWGCWC 1 cut(s) 988
BsiSI CCGG 4 cut(s) 159, 647, 1933, 2230
BslFI GGGAC 3 cut(s) 860, 1646, 1801
BslI CCNNNNNNNGG 3 cut(s) 252, 683, 1222
BsmAI GTCTC 3 cut(s) 186, 2131, 2185
BsmFI GGGAC 3 cut(s) 860, 1646, 1801
BsmI GAATGC 2 cut(s) 1482, 1678
BsnI GGCC 1 cut(s) 1559
Bso31I GGTCTC 2 cut(s) 186, 2185
Bsp119I TTCGAA 1 cut(s) 584
Bsp1286I GDGCHC 1 cut(s) 988
Bsp13I TCCGGA 1 cut(s) 2229
Bsp1407I TGTACA 1 cut(s) 456
Bsp1720I GCTNAGC 1 cut(s) 183
Bsp19I CCATGG 1 cut(s) 1624
BspACI CCGC 3 cut(s) 684, 1556, 1599
BspANI GGCC 1 cut(s) 1559
BspCNI CTCAG 8 cut(s) 196, 201, 224, 618, 898, 993, 1300, 2141
BspEI TCCGGA 1 cut(s) 2229
BspHI TCATGA 1 cut(s) 1021
BspLI GGNNCC 1 cut(s) 746
BspOI GCTAGC 1 cut(s) 1295
BspPI GGATC 9 cut(s) 454, 1005, 1264, 1312, 1615, 1970, 2080, 2220, 2258
BspQI GCTCTTC 1 cut(s) 234
BspT104I TTCGAA 1 cut(s) 584
BspTNI GGTCTC 2 cut(s) 186, 2185
BsrDI GCAATG 3 cut(s) 247, 775, 1233
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 1624, 1958
BssNAI GTATAC 1 cut(s) 354
BssT1I CCWWGG 2 cut(s) 1624, 1958
Bst1107I GTATAC 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 2268
Bst4CI ACNGT 7 cut(s) 522, 565, 581, 868, 1325, 1333, 2001
Bst6I CTCTTC 3 cut(s) 234, 699, 1226
BstAUI TGTACA 1 cut(s) 456
BstBAI YACGTR 1 cut(s) 2123
BstBI TTCGAA 1 cut(s) 584
BstC8I GCNNGC 8 cut(s) 678, 682, 1008, 1293, 1480, 1596, 2240, 2244
BstDEI CTNAG 9 cut(s) 183, 188, 211, 626, 885, 1001, 1287, 2081, 2128
BstDSI CCRYGG 1 cut(s) 1624
BstENI CCTNNNNNAGG 1 cut(s) 1220
BstF5I GGATG 5 cut(s) 640, 1153, 1756, 1941, 2286
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 1 cut(s) 274
BstMAI GTCTC 3 cut(s) 186, 2131, 2185
BstMWI GCNNNNNNNGC 3 cut(s) 383, 1871, 2093
BstNI CCWGG 1 cut(s) 2268
BstNSI RCATGY 2 cut(s) 484, 2242
BstSCI CCNGG 1 cut(s) 2266
BstSFI CTRYAG 1 cut(s) 577
BstV1I GCAGC 3 cut(s) 370, 1462, 2207
BstV2I GAAGAC 1 cut(s) 142
BstX2I RGATCY 4 cut(s) 997, 1620, 2072, 2263
BstYI RGATCY 4 cut(s) 997, 1620, 2072, 2263
BstZ17I GTATAC 1 cut(s) 354
BsuI GTATCC 1 cut(s) 63
BsuRI GGCC 1 cut(s) 1559
BtgI CCRYGG 1 cut(s) 1624
BtsCI GGATG 5 cut(s) 640, 1153, 1756, 1941, 2286
BtsIMutI CAGTG 5 cut(s) 93, 315, 372, 864, 1321
Cac8I GCNNGC 8 cut(s) 678, 682, 1008, 1293, 1480, 1596, 2240, 2244
CciI TCATGA 1 cut(s) 1021
CfoI GCGC 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 745, 1764
Csp6I GTAC 5 cut(s) 24, 36, 457, 787, 880
CviQI GTAC 5 cut(s) 24, 36, 457, 787, 880
DdeI CTNAG 9 cut(s) 183, 188, 211, 626, 885, 1001, 1287, 2081, 2128
DraI TTTAAA 1 cut(s) 901
DraIII CACNNNGTG 1 cut(s) 2003
EaeI YGGCCR 1 cut(s) 1557
Eam1104I CTCTTC 3 cut(s) 234, 699, 1226
EarI CTCTTC 3 cut(s) 234, 699, 1226
Eco130I CCWWGG 2 cut(s) 1624, 1958
Eco31I GGTCTC 2 cut(s) 186, 2185
Eco47I GGWCC 2 cut(s) 745, 1764
Eco47III AGCGCT 1 cut(s) 273
Eco57I CTGAAG 2 cut(s) 114, 348
Eco72I CACGTG 1 cut(s) 2123
EcoNI CCTNNNNNAGG 1 cut(s) 1220
EcoRI GAATTC 3 cut(s) 256, 1162, 2149
EcoRII CCWGG 1 cut(s) 2266
EcoT14I CCWWGG 2 cut(s) 1624, 1958
EcoT22I ATGCAT 2 cut(s) 472, 2240
ErhI CCWWGG 2 cut(s) 1624, 1958
FalI AAGNNNNNCTT 2 cut(s) 1027, 1059
FaqI GGGAC 3 cut(s) 860, 1646, 1801
FauNDI CATATG 3 cut(s) 1134, 1737, 1792
FbaI TGATCA 4 cut(s) 369, 1129, 1170, 1855
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 5 cut(s) 384, 1476, 1557, 1599, 2196
FokI GGATG 5 cut(s) 647, 1160, 1763, 1948, 2293
Fsp4HI GCNGC 5 cut(s) 384, 1476, 1557, 1599, 2196
FspBI CTAG 6 cut(s) 1292, 1421, 1695, 1919, 1959, 2097
GlaI GCGC 1 cut(s) 273
GluI GCNGC 5 cut(s) 384, 1476, 1557, 1599, 2196
HaeII RGCGCY 1 cut(s) 275
HaeIII GGCC 1 cut(s) 1559
HapII CCGG 4 cut(s) 159, 647, 1933, 2230
HhaI GCGC 1 cut(s) 274
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HincII GTYRAC 1 cut(s) 1987
HindII GTYRAC 1 cut(s) 1987
HindIII AAGCTT 1 cut(s) 603
HpaII CCGG 4 cut(s) 159, 647, 1933, 2230
HphI GGTGA 5 cut(s) 509, 707, 943, 1768, 1808
Hpy166II GTNNAC 5 cut(s) 112, 354, 500, 1303, 1987
Hpy188I TCNGA 9 cut(s) 191, 269, 433, 451, 714, 1002, 1201, 1657, 2131
Hpy188III TCNNGA 8 cut(s) 132, 305, 398, 829, 1022, 1445, 1853, 2230
Hpy8I GTNNAC 5 cut(s) 112, 354, 500, 1303, 1987
HpyAV CCTTC 7 cut(s) 372, 1199, 1532, 1741, 1859, 1907, 2044
HpyCH4III ACNGT 7 cut(s) 522, 565, 581, 868, 1325, 1333, 2001
HpyCH4IV ACGT 1 cut(s) 2122
HpyF10VI GCNNNNNNNGC 3 cut(s) 383, 1871, 2093
HpyF3I CTNAG 9 cut(s) 183, 188, 211, 626, 885, 1001, 1287, 2081, 2128
HpySE526I ACGT 1 cut(s) 2122
HspAI GCGC 1 cut(s) 272
Kpn2I TCCGGA 1 cut(s) 2229
Ksp22I TGATCA 4 cut(s) 369, 1129, 1170, 1855
LguI GCTCTTC 1 cut(s) 234
Lsp1109I GCAGC 3 cut(s) 370, 1462, 2207
LweI GCATC 6 cut(s) 802, 1138, 1248, 1536, 1543, 2047
MaeI CTAG 6 cut(s) 1292, 1421, 1695, 1919, 1959, 2097
MaeII ACGT 1 cut(s) 2122
MaeIII GTNAC 5 cut(s) 124, 642, 1453, 1756, 1796
MfeI CAATTG 1 cut(s) 2144
MflI RGATCY 4 cut(s) 997, 1620, 2072, 2263
MhlI GDGCHC 1 cut(s) 988
MlyI GAGTC 4 cut(s) 842, 899, 1244, 2247
MmeI TCCRAC 1 cut(s) 839
Mph1103I ATGCAT 2 cut(s) 472, 2240
MroI TCCGGA 1 cut(s) 2229
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 6 cut(s) 806, 900, 1188, 1221, 1511, 1637
MslI CAYNNNNRTG 4 cut(s) 320, 423, 753, 1656
MspI CCGG 4 cut(s) 159, 647, 1933, 2230
MspR9I CCNGG 1 cut(s) 2268
MunI CAATTG 1 cut(s) 2144
Mva1269I GAATGC 2 cut(s) 1482, 1678
MvaI CCWGG 1 cut(s) 2268
MwoI GCNNNNNNNGC 3 cut(s) 383, 1871, 2093
NcoI CCATGG 1 cut(s) 1624
NdeI CATATG 3 cut(s) 1134, 1737, 1792
NheI GCTAGC 1 cut(s) 1291
NlaIV GGNNCC 1 cut(s) 746
NmuCI GTSAC 3 cut(s) 124, 1756, 1796
NsiI ATGCAT 2 cut(s) 472, 2240
NspI RCATGY 2 cut(s) 484, 2242
NspV TTCGAA 1 cut(s) 584
PaeI GCATGC 1 cut(s) 2242
PagI TCATGA 1 cut(s) 1021
PciI ACATGT 1 cut(s) 480
PciSI GCTCTTC 1 cut(s) 234
PctI GAATGC 2 cut(s) 1482, 1678
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 6 cut(s) 650, 1344, 1412, 1519, 2226, 2287
PfoI TCCNGGA 1 cut(s) 2266
PkrI GCNGC 5 cut(s) 385, 1477, 1558, 1600, 2197
PleI GAGTC 4 cut(s) 842, 899, 1244, 2247
PmaCI CACGTG 1 cut(s) 2123
PmlI CACGTG 1 cut(s) 2123
PpsI GAGTC 4 cut(s) 842, 899, 1244, 2247
Ppu21I YACGTR 1 cut(s) 2123
PscI ACATGT 1 cut(s) 480
Psp6I CCWGG 1 cut(s) 2266
PspCI CACGTG 1 cut(s) 2123
PspGI CCWGG 1 cut(s) 2266
PspN4I GGNNCC 1 cut(s) 746
PspPI GGNCC 2 cut(s) 745, 1764
PsuI RGATCY 4 cut(s) 997, 1620, 2072, 2263
RsaI GTAC 5 cut(s) 25, 37, 458, 788, 881
RsaNI GTAC 5 cut(s) 24, 36, 457, 787, 880
RseI CAYNNNNRTG 4 cut(s) 320, 423, 753, 1656
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 6 cut(s) 806, 900, 1188, 1221, 1511, 1637
SatI GCNGC 5 cut(s) 384, 1476, 1557, 1599, 2196
Sau96I GGNCC 2 cut(s) 745, 1764
SchI GAGTC 4 cut(s) 842, 899, 1244, 2247
ScrFI CCNGG 1 cut(s) 2268
SduI GDGCHC 1 cut(s) 988
SfaNI GCATC 6 cut(s) 802, 1138, 1248, 1536, 1543, 2047
SfcI CTRYAG 1 cut(s) 577
SfuI TTCGAA 1 cut(s) 584
SinI GGWCC 2 cut(s) 745, 1764
SmiMI CAYNNNNRTG 4 cut(s) 320, 423, 753, 1656
SmlI CTYRAG 2 cut(s) 16, 2016
SmoI CTYRAG 2 cut(s) 16, 2016
SpeI ACTAGT 1 cut(s) 1420
SphI GCATGC 1 cut(s) 2242
SsiI CCGC 3 cut(s) 684, 1556, 1599
SspMI CTAG 6 cut(s) 1292, 1421, 1695, 1919, 1959, 2097
StyD4I CCNGG 1 cut(s) 2266
StyI CCWWGG 2 cut(s) 1624, 1958
TaaI ACNGT 7 cut(s) 522, 565, 581, 868, 1325, 1333, 2001
TaiI ACGT 1 cut(s) 2125
TaqI TCGA 6 cut(s) 340, 584, 828, 1347, 1965, 2092
TatI WGTACW 3 cut(s) 23, 456, 786
TauI GCSGC 2 cut(s) 1559, 1601
TfiI GAWTC 6 cut(s) 650, 1344, 1412, 1519, 2226, 2287
Tru1I TTAA 6 cut(s) 806, 900, 1188, 1221, 1511, 1637
Tru9I TTAA 6 cut(s) 806, 900, 1188, 1221, 1511, 1637
TscAI CASTG 5 cut(s) 100, 322, 372, 871, 1328
TseFI GTSAC 3 cut(s) 124, 1756, 1796
TseI GCWGC 3 cut(s) 383, 1475, 2195
Tsp45I GTSAC 3 cut(s) 124, 1756, 1796
TspGWI ACGGA 4 cut(s) 45, 136, 965, 1815
TspRI CASTG 5 cut(s) 100, 322, 372, 871, 1328
VpaK11BI GGWCC 2 cut(s) 745, 1764
XagI CCTNNNNNAGG 1 cut(s) 1220
XapI RAATTY 7 cut(s) 256, 612, 794, 913, 938, 1162, 2149
XceI RCATGY 2 cut(s) 484, 2242
XmaJI CCTAGG 1 cut(s) 1958
XmiI GTMKAC 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 6 cut(s) 1292, 1421, 1695, 1919, 1959, 2097
Zsp2I ATGCAT 2 cut(s) 472, 2240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.