MD02G1241700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006827
Physical Location & Seq
Forward (+)
29040186 .. 29044575
4390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1241700.v1.1.491

Sequence Viewer

Length: 1482 bp
ATGCATTCTATAAAGGGTAGTTGGGTTGGGCAGACATTCGCCCTAGCTAAAAATAATGAATCTGAAGGAAGGAGGTCCAGAATTCGGCGTTCAAAGGAAGAGAGGAAGGCAATGGTTGAATCCTTTATAAAAAAGTATCAGAAAATAAACAATGGGAGTTTTCCATCACTTAATCTGACCCACAAAGAAGTTGGTGGGTCTTTCTACACTGTACGAGAAATTGTTCGAGATATAATTCAGGAAAATAGAGTGCTTGGTCCTGCTAAGTTTACTGTAGAGGAGCAGCAGACCATTGATCAGTTTTGGGAACACAATCCATTGGGTACCATTGCTGCAGAACCACAAAATACTTTGTCAATATCATCAAATGAATTGCTGTTCGTCACCAATCAAAATCAGGGTGTAATTGAACAACTTGTTTTCGCTTCCGATGGGCATTTAGTTGCACCTGAACATCAGATGTTTGAGAATGGACGAATCATCAATGGTACTCAAGTAGAAGTGAATGACAAAGAATTTAATGAACCCAAAAGTACAGAGTTACAAGCAAATGGACCTGTGGAAATAGAGAAGCATGTTGCTGTTGAATCTATAGATTCTAATGGGCATTGTATTGGTCCTGAATATCAGAGTTTTGACAATGGACTAATCAATGGTAGCCAGATAGATAGGAAAGATAAAGAAAGGGAGGAACGAATGTGCACAGAACTGCAAACAGTTGAACCTTCGGAAGCAGAGAAGAATGCTGAAGACGTTCGGGCAACATCCAAGTCTAATGTGAATCCTTTAACAGCAGATGTGATAGTGGAGACATTCCCATTGAAGCCAGTAAATGCGACACCTGAGGGCTTGGATGGAAGGTTACAAGAAGTAACGGATTTGACTAAGTCTTCCAAGGACAAGGGAACTAGAGGGATGGAGTTAGCTGGTGTTGATAGTTTGCAAGTGGATAGATTATATTCCATGAAGAGCATGGATGATAATGTAGCGATGAATCTTTCAAGTCCATTGGTTGAAAACAAATCCAGCCCAGTGGATGAAGCACTAGGAAATGCCATAGATCCATCAGTTGAAAGCTCAAGTTACTCAACCTTTAATGAAGGTATTGTGCATGAAACAGGAAGCACAGACCTTAATGTCAAATCACCTCATAAAGATGTTCCAACCTCTGAGATCTCTAACCAAAACCAGCTGACCGCGGGAACTAAGGCTGACAAAGCTCCAGATAGCTTACATACAAAGATTACCAACGGTACCAGTGGTAGTAGTGAGCAATCGAAAACCAAAGAGGTGGTTGTGATTGAAGATGAAGTTGATGTCCAGACCAGCGGTAGCTTGAAGAAGGGAAGCAAACCAACTTTGGACAGAATTAACCTTGAATCATGGGAAGGTGCATCTAAAAAGACCAGACCAGAAGGTAACCCACTTTGGGATGTATTTAAAGCGTTTTTAGACGCCCTTGTGAAATTCTGGTCCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

494

Amino Acids

54.46

Weight (kDa)

5.01

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_AT3G52170 PF25896 31 - 79 1e-23 Helix-turn-helix domain in AT3G52170-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
AasI GACNNNNNNGTC 1 cut(s) 1136
Acc65I GGTACC 2 cut(s) 323, 1253
AccB1I GGYRCC 2 cut(s) 323, 1253
AccII CGCG 1 cut(s) 1199
AciI CCGC 3 cut(s) 1197, 1199, 1329
AclWI GGATC 1 cut(s) 1055
AcsI RAATTY 3 cut(s) 81, 515, 1466
AcuI CTGAAG 2 cut(s) 84, 768
AcyI GRCGYC 1 cut(s) 1455
AfaI GTAC 5 cut(s) 213, 325, 490, 535, 1255
AfiI CCNNNNNNNGG 2 cut(s) 84, 1429
AluBI AGCT 7 cut(s) 47, 926, 1077, 1192, 1220, 1230, 1335
AluI AGCT 7 cut(s) 47, 926, 1077, 1192, 1220, 1230, 1335
Alw21I GWGCWC 1 cut(s) 704
Alw26I GTCTC 1 cut(s) 803
Alw44I GTGCAC 1 cut(s) 700
AlwI GGATC 1 cut(s) 1055
ApaLI GTGCAC 1 cut(s) 700
ApeKI GCWGC 2 cut(s) 283, 332
ApoI RAATTY 3 cut(s) 81, 515, 1466
Asp700I GAANNNNTTC 2 cut(s) 222, 753
Asp718I GGTACC 2 cut(s) 323, 1253
AspS9I GGNCC 5 cut(s) 75, 257, 554, 617, 1473
AsuHPI GGTGA 2 cut(s) 376, 1137
AvaII GGWCC 5 cut(s) 75, 257, 554, 617, 1473
AxyI CCTNAGG 1 cut(s) 843
BaeGI GKGCMC 1 cut(s) 704
BaeI ACNNNNGTAYC 2 cut(s) 480, 513
BanI GGYRCC 2 cut(s) 323, 1253
BbsI GAAGAC 2 cut(s) 756, 882
Bbv12I GWGCWC 1 cut(s) 704
BbvI GCAGC 2 cut(s) 295, 319
BccI CCATC 5 cut(s) 172, 425, 848, 910, 1072
BclI TGATCA 1 cut(s) 295
BcoDI GTCTC 1 cut(s) 803
BfaI CTAG 3 cut(s) 44, 909, 1046
BfmI CTRYAG 3 cut(s) 273, 333, 591
BglII AGATCT 1 cut(s) 1173
BisI GCNGC 2 cut(s) 284, 333
BlsI GCNGC 2 cut(s) 285, 334
Bme18I GGWCC 5 cut(s) 75, 257, 554, 617, 1473
BmgT120I GGNCC 5 cut(s) 75, 257, 554, 617, 1473
BmiI GGNNCC 2 cut(s) 325, 1255
BmrI ACTGGG 1 cut(s) 1025
BmsI GCATC 1 cut(s) 1403
BmuI ACTGGG 1 cut(s) 1025
BpiI GAAGAC 2 cut(s) 756, 882
BpmI CTGGAG 1 cut(s) 1206
BpuEI CTTGAG 2 cut(s) 477, 1063
BsaHI GRCGYC 1 cut(s) 1455
BsaJI CCNNGG 2 cut(s) 894, 1197
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 1429
Bse1I ACTGG 3 cut(s) 827, 1031, 1257
Bse21I CCTNAGG 1 cut(s) 843
Bse3DI GCAATG 2 cut(s) 117, 327
BseDI CCNNGG 2 cut(s) 894, 1197
BseGI GGATG 6 cut(s) 764, 859, 921, 982, 1042, 1438
BseLI CCNNNNNNNGG 2 cut(s) 84, 1429
BseMI GCAATG 2 cut(s) 117, 327
BseMII CTCAG 2 cut(s) 834, 1161
BseNI ACTGG 3 cut(s) 827, 1031, 1257
BseRI GAGGAG 1 cut(s) 293
BseSI GKGCMC 1 cut(s) 704
BseXI GCAGC 2 cut(s) 295, 319
Bsh1236I CGCG 1 cut(s) 1199
BshNI GGYRCC 2 cut(s) 323, 1253
BsiHKAI GWGCWC 1 cut(s) 704
BslI CCNNNNNNNGG 2 cut(s) 84, 1429
BsmAI GTCTC 1 cut(s) 803
BsmI GAATGC 2 cut(s) 4, 748
Bsp1286I GDGCHC 1 cut(s) 704
Bsp143I GATC 3 cut(s) 295, 1060, 1173
BspACI CCGC 3 cut(s) 1197, 1199, 1329
BspCNI CTCAG 2 cut(s) 835, 1162
BspFNI CGCG 1 cut(s) 1199
BspLI GGNNCC 2 cut(s) 325, 1255
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 1 cut(s) 1055
BspQI GCTCTTC 1 cut(s) 962
BspT107I GGYRCC 2 cut(s) 323, 1253
BsrDI GCAATG 2 cut(s) 117, 327
BsrI ACTGG 3 cut(s) 827, 1031, 1257
BssECI CCNNGG 2 cut(s) 894, 1197
BssMI GATC 3 cut(s) 295, 1060, 1173
BssNI GRCGYC 1 cut(s) 1455
BssT1I CCWWGG 1 cut(s) 894
Bst4CI ACNGT 4 cut(s) 211, 274, 718, 1253
Bst6I CTCTTC 2 cut(s) 93, 962
BstACI GRCGYC 1 cut(s) 1455
BstDEI CTNAG 5 cut(s) 264, 843, 885, 1170, 1206
BstDSI CCRYGG 1 cut(s) 1197
BstEII GGTNACC 1 cut(s) 1418
BstF5I GGATG 6 cut(s) 764, 859, 921, 982, 1042, 1438
BstFNI CGCG 1 cut(s) 1199
BstKTI GATC 3 cut(s) 298, 1063, 1176
BstMAI GTCTC 1 cut(s) 803
BstMBI GATC 3 cut(s) 295, 1060, 1173
BstMWI GCNNNNNNNGC 1 cut(s) 1217
BstNSI RCATGY 1 cut(s) 578
BstPI GGTNACC 1 cut(s) 1418
BstSFI CTRYAG 3 cut(s) 273, 333, 591
BstSLI GKGCMC 1 cut(s) 704
BstUI CGCG 1 cut(s) 1199
BstV1I GCAGC 2 cut(s) 295, 319
BstV2I GAAGAC 2 cut(s) 756, 882
BstX2I RGATCY 2 cut(s) 1060, 1173
BstXI CCANNNNNNTGG 2 cut(s) 1033, 1291
BstYI RGATCY 2 cut(s) 1060, 1173
Bsu36I CCTNAGG 1 cut(s) 843
BtgI CCRYGG 1 cut(s) 1197
BtgZI GCGATG 1 cut(s) 1004
BtsCI GGATG 6 cut(s) 764, 859, 921, 982, 1042, 1438
BtsIMutI CAGTG 3 cut(s) 207, 1038, 1264
Cfr13I GGNCC 5 cut(s) 75, 257, 554, 617, 1473
Cfr42I CCGCGG 1 cut(s) 1200
CseI GACGC 1 cut(s) 1463
Csp6I GTAC 5 cut(s) 212, 324, 489, 534, 1254
CviAII CATG 5 cut(s) 575, 964, 973, 1112, 1383
CviQI GTAC 5 cut(s) 212, 324, 489, 534, 1254
DdeI CTNAG 5 cut(s) 264, 843, 885, 1170, 1206
DpnI GATC 3 cut(s) 297, 1062, 1175
DpnII GATC 3 cut(s) 295, 1060, 1173
DraI TTTAAA 1 cut(s) 1441
DrdI GACNNNNNNGTC 1 cut(s) 1136
DseDI GACNNNNNNGTC 1 cut(s) 1136
Eam1104I CTCTTC 2 cut(s) 93, 962
EarI CTCTTC 2 cut(s) 93, 962
Eco130I CCWWGG 1 cut(s) 894
Eco47I GGWCC 5 cut(s) 75, 257, 554, 617, 1473
Eco57I CTGAAG 2 cut(s) 84, 768
Eco81I CCTNAGG 1 cut(s) 843
Eco91I GGTNACC 1 cut(s) 1418
EcoO65I GGTNACC 1 cut(s) 1418
EcoRI GAATTC 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 894
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 894
FaeI CATG 5 cut(s) 578, 967, 976, 1115, 1386
FatI CATG 5 cut(s) 574, 963, 972, 1111, 1382
FauI CCCGC 1 cut(s) 1192
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 2 cut(s) 284, 333
FokI GGATG 6 cut(s) 751, 866, 928, 989, 1049, 1445
Fsp4HI GCNGC 2 cut(s) 284, 333
FspBI CTAG 3 cut(s) 44, 909, 1046
GluI GCNGC 2 cut(s) 284, 333
GsuI CTGGAG 1 cut(s) 1206
HgaI GACGC 1 cut(s) 1463
Hin1I GRCGYC 1 cut(s) 1455
Hin1II CATG 5 cut(s) 578, 967, 976, 1115, 1386
HinfI GANTC 8 cut(s) 59, 119, 477, 587, 596, 781, 994, 1379
HphI GGTGA 2 cut(s) 376, 1137
Hpy166II GTNNAC 2 cut(s) 270, 702
Hpy188I TCNGA 9 cut(s) 64, 141, 177, 430, 459, 630, 730, 1171, 1477
Hpy188III TCNNGA 6 cut(s) 78, 227, 239, 620, 1223, 1321
Hpy8I GTNNAC 2 cut(s) 270, 702
HpyAV CCTTC 9 cut(s) 59, 63, 100, 735, 852, 1094, 1336, 1382, 1409
HpyCH4III ACNGT 4 cut(s) 211, 274, 718, 1253
HpyCH4IV ACGT 1 cut(s) 753
HpyCH4V TGCA 8 cut(s) 4, 335, 446, 702, 712, 943, 1111, 1394
HpyF10VI GCNNNNNNNGC 1 cut(s) 1217
HpyF3I CTNAG 5 cut(s) 264, 843, 885, 1170, 1206
HpySE526I ACGT 1 cut(s) 753
Hsp92I GRCGYC 1 cut(s) 1455
Hsp92II CATG 5 cut(s) 578, 967, 976, 1115, 1386
KpnI GGTACC 2 cut(s) 327, 1257
Ksp22I TGATCA 1 cut(s) 295
KspI CCGCGG 1 cut(s) 1200
Kzo9I GATC 3 cut(s) 295, 1060, 1173
LguI GCTCTTC 1 cut(s) 962
LmnI GCTCC 2 cut(s) 280, 1225
Lsp1109I GCAGC 2 cut(s) 295, 319
LweI GCATC 1 cut(s) 1403
MaeI CTAG 3 cut(s) 44, 909, 1046
MaeII ACGT 1 cut(s) 753
MaeIII GTNAC 6 cut(s) 382, 540, 861, 871, 1082, 1418
MalI GATC 3 cut(s) 297, 1062, 1175
MboI GATC 3 cut(s) 295, 1060, 1173
MboII GAAGA 7 cut(s) 110, 751, 761, 882, 979, 1316, 1351
MflI RGATCY 2 cut(s) 1060, 1173
MhlI GDGCHC 1 cut(s) 704
MluCI AATT 8 cut(s) 81, 219, 234, 371, 405, 515, 1368, 1466
MmeI TCCRAC 1 cut(s) 1187
MnlI CCTC 9 cut(s) 66, 96, 271, 682, 838, 905, 1158, 1177, 1282
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 222, 753
MseI TTAA 7 cut(s) 171, 519, 788, 1095, 1134, 1371, 1440
MslI CAYNNNNRTG 1 cut(s) 1155
MspA1I CMGCKG 3 cut(s) 1192, 1199, 1329
Mva1269I GAATGC 2 cut(s) 4, 748
MvnI CGCG 1 cut(s) 1199
MwoI GCNNNNNNNGC 1 cut(s) 1217
NdeII GATC 3 cut(s) 295, 1060, 1173
NlaIII CATG 5 cut(s) 578, 967, 976, 1115, 1386
NlaIV GGNNCC 2 cut(s) 325, 1255
NmuCI GTSAC 1 cut(s) 382
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 578
PciSI GCTCTTC 1 cut(s) 962
PctI GAATGC 2 cut(s) 4, 748
PdmI GAANNNNTTC 2 cut(s) 222, 753
PfeI GAWTC 8 cut(s) 59, 119, 477, 587, 596, 781, 994, 1379
PflFI GACNNNGTC 1 cut(s) 886
PkrI GCNGC 2 cut(s) 285, 334
PsiI TTATAA 1 cut(s) 128
PspEI GGTNACC 1 cut(s) 1418
PspN4I GGNNCC 2 cut(s) 325, 1255
PspPI GGNCC 5 cut(s) 75, 257, 554, 617, 1473
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 2 cut(s) 1060, 1173
PsyI GACNNNGTC 1 cut(s) 886
PvuII CAGCTG 1 cut(s) 1192
RsaI GTAC 5 cut(s) 213, 325, 490, 535, 1255
RsaNI GTAC 5 cut(s) 212, 324, 489, 534, 1254
RseI CAYNNNNRTG 1 cut(s) 1155
SacII CCGCGG 1 cut(s) 1200
SapI GCTCTTC 1 cut(s) 962
SaqAI TTAA 7 cut(s) 171, 519, 788, 1095, 1134, 1371, 1440
SatI GCNGC 2 cut(s) 284, 333
Sau3AI GATC 3 cut(s) 295, 1060, 1173
Sau96I GGNCC 5 cut(s) 75, 257, 554, 617, 1473
SduI GDGCHC 1 cut(s) 704
SfaNI GCATC 1 cut(s) 1403
SfcI CTRYAG 3 cut(s) 273, 333, 591
Sfr303I CCGCGG 1 cut(s) 1200
SgrBI CCGCGG 1 cut(s) 1200
SinI GGWCC 5 cut(s) 75, 257, 554, 617, 1473
SmiMI CAYNNNNRTG 1 cut(s) 1155
SmlI CTYRAG 2 cut(s) 492, 1078
SmoI CTYRAG 2 cut(s) 492, 1078
Sse9I AATT 8 cut(s) 81, 219, 234, 371, 405, 515, 1368, 1466
SsiI CCGC 3 cut(s) 1197, 1199, 1329
SspMI CTAG 3 cut(s) 44, 909, 1046
StyI CCWWGG 1 cut(s) 894
TaaI ACNGT 4 cut(s) 211, 274, 718, 1253
TaiI ACGT 1 cut(s) 756
TaqI TCGA 2 cut(s) 226, 1277
TasI AATT 8 cut(s) 81, 219, 234, 371, 405, 515, 1368, 1466
TatI WGTACW 1 cut(s) 533
TfiI GAWTC 8 cut(s) 59, 119, 477, 587, 596, 781, 994, 1379
Tru1I TTAA 7 cut(s) 171, 519, 788, 1095, 1134, 1371, 1440
Tru9I TTAA 7 cut(s) 171, 519, 788, 1095, 1134, 1371, 1440
TscAI CASTG 3 cut(s) 214, 1038, 1264
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 2 cut(s) 283, 332
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 9 cut(s) 72, 384, 537, 980, 1007, 1053, 1113, 1128, 1323
TspGWI ACGGA 1 cut(s) 890
TspRI CASTG 3 cut(s) 214, 1038, 1264
Tth111I GACNNNGTC 1 cut(s) 886
VneI GTGCAC 1 cut(s) 700
VpaK11BI GGWCC 5 cut(s) 75, 257, 554, 617, 1473
XapI RAATTY 3 cut(s) 81, 515, 1466
XceI RCATGY 1 cut(s) 578
XcmI CCANNNNNNNNNTGG 2 cut(s) 188, 970
XmnI GAANNNNTTC 2 cut(s) 222, 753
XspI CTAG 3 cut(s) 44, 909, 1046
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.