Rw1G011900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006827
Physical Location & Seq
Reverse (-)
26746962 .. 26751226
4265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011900.1

Sequence Viewer

Length: 1455 bp
ATGCATGCTATAAAGGGTGGCTGGGTTGGGCAAACATTTGCCCTAGCTAGAAACAATGAATCTGAAGGAAGGAAGTCCCGAATTCGGCGTTCAAAGGAGGAGAGGAAGGAAATGGTTGAAGCCTTTATAAAAAAGTATCAGATTCTACACAACGGGAGTTTTCCATCACTTAATCTGACCCACAAGGAAGTTGGTGGATCTTTCTACACTGTACGAGAAATTGTCCGAGATGTAATCCAAGAAAATAGAGTGCTGGGTCCTGCTAAGTTCGTTGCAGAGGATCAGACTATTGATCAGTTCTTGGAACGTAATCCACTTGGTTCGATTGCTGCAGAACCTCAAAATGCTTTGTCAGAAGCATCAAATGATTCTCAGTTTGCCAACAATCAAAATCAGGGTACAGTTCAAGAAATGGTTTTGGCTTCTGATGGTCATTCGTTTACTCTTGAACATCAGATTCTCGACCATGGCCGAGCCATCAATGGCACTGCAGCAGAAGTGAATAGCAAAGAAGTGGAGAGTAAAGAGTTGCAAGTAACTGTGTCAGTGGAAACAGAGAAGAAGGTAGCTGAAGAATCTGTAGTTTCTGATGGGAATTGTATTTGTCATGAATATCAGATGTTTGACAATGGCGGCCAGGTAGATCAGGAAGACAAACAAACTGAGGAACTGATATGTGCAGAGCATAGAACAAGTGTGCCTTTAGAAGTAGAGAAAAATGTTGAGGAAGTTTTGGCAATATCTAGGTCTAAAGTGACCTCCATAGAAGCAGATGTAATAGTTGAGACATTCCCCTTAACGCCTGTTACTAGGACTACTGAGAGCTTGGATGGAAAGGTAGAATTAGGAAATTTTGGTATATCTACTGAGGATAAAGGAACCAAGGGGATGGGGTTAGCCACTGGTGTTGATAGTACGCTGTCGGATACAGTATATTCCATGAATAGCTTGGTCGATGATAAAGTAGTGGTGAAAAGCCCCTTGGTTGGAAACAAATCCAGCTCAGTAAATGAGGAAGCCTTAGAAATTGTCAGAGACCCATCACTGGAGACCTCAAATCGCTCAACCCTTGAAGGAAATATTATACATGAAAAAGGAAGCACAGAACTTAAGGTCAAAGCACCTGGAAATGATGTTCCTATATCTGAGAGCTTTGAACAAATTGAAGGGACTGCTGGAGCTAAGATAATAAAAGCTCCAGATACAAAGAACCGTAATGGTACCAGCGTTTGCGATGGGCTGTCACAAACCAAAGAGGTGCTTGTGATTGAAGATGAAGTTGGTGTGCACTCCAGCGCAGGGTTGCAAAAAGGAAGCAACACTTTAGACAGGATTAGTCTCGAATCGTGGCAAAAGGTGTCTAGAAATTCCACAAATCGAGAAGGCAAATCACTTTGGGCTGTATTTAAAGAGTACATAGACGCCATTGTGAAGTTTTGGTCTAATGAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

484

Amino Acids

53.08

Weight (kDa)

4.94

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_AT3G52170 PF25896 31 - 79 4.7e-22 Helix-turn-helix domain in AT3G52170-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
Acc65I GGTACC 1 cut(s) 1220
AccB1I GGYRCC 1 cut(s) 1220
AciI CCGC 1 cut(s) 633
AclWI GGATC 2 cut(s) 205, 288
AcoI YGGCCR 2 cut(s) 469, 634
AcsI RAATTY 3 cut(s) 81, 850, 1366
AcuI CTGAAG 2 cut(s) 84, 591
AcyI GRCGYC 1 cut(s) 1422
AfaI GTAC 5 cut(s) 213, 400, 916, 1222, 1415
AfiI CCNNNNNNNGG 4 cut(s) 84, 986, 1045, 1299
AflII CTTAAG 1 cut(s) 1109
AgsI TTSAA 8 cut(s) 93, 119, 407, 449, 1073, 1157, 1166, 1271
AjnI CCWGG 2 cut(s) 636, 1123
AjuI GAANNNNNNNTTGG 4 cut(s) 965, 997, 1263, 1295
AloI GAACNNNNNNTCC 2 cut(s) 1119, 1151
AluBI AGCT 9 cut(s) 47, 569, 825, 948, 1002, 1152, 1181, 1196, 1450
AluI AGCT 9 cut(s) 47, 569, 825, 948, 1002, 1152, 1181, 1196, 1450
Alw21I GWGCWC 1 cut(s) 1290
Alw26I GTCTC 4 cut(s) 779, 1029, 1043, 1343
Alw44I GTGCAC 1 cut(s) 1286
AlwI GGATC 2 cut(s) 205, 288
AoxI GGCC 2 cut(s) 469, 634
ApaLI GTGCAC 1 cut(s) 1286
ApeKI GCWGC 2 cut(s) 329, 491
ApoI RAATTY 3 cut(s) 81, 850, 1366
Asp718I GGTACC 1 cut(s) 1220
AspLEI GCGC 1 cut(s) 1298
AspS9I GGNCC 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 982
AvaII GGWCC 1 cut(s) 257
BaeGI GKGCMC 1 cut(s) 1290
BaeI ACNNNNGTAYC 2 cut(s) 1212, 1245
BanI GGYRCC 1 cut(s) 1220
BbsI GAAGAC 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 1290
BbvI GCAGC 2 cut(s) 316, 503
BccI CCATC 8 cut(s) 172, 422, 485, 584, 824, 883, 1048, 1229
BciT130I CCWGG 2 cut(s) 638, 1125
BciVI GTATCC 1 cut(s) 919
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 4 cut(s) 779, 1029, 1043, 1343
BfaI CTAG 5 cut(s) 44, 48, 744, 810, 1362
BfmI CTRYAG 3 cut(s) 330, 489, 579
BfrI CTTAAG 1 cut(s) 1109
BfuI GTATCC 1 cut(s) 919
BisI GCNGC 3 cut(s) 330, 492, 634
BlsI GCNGC 3 cut(s) 331, 493, 635
Bme1390I CCNGG 2 cut(s) 638, 1125
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 3 cut(s) 258, 880, 1222
BmrFI CCNGG 2 cut(s) 638, 1125
BmsI GCATC 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 657
BpmI CTGGAG 4 cut(s) 1067, 1182, 1197, 1276
BsaHI GRCGYC 1 cut(s) 1422
BsaI GGTCTC 2 cut(s) 1029, 1043
BsaJI CCNNGG 3 cut(s) 466, 882, 981
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 986, 1045, 1299
Bse1I ACTGG 2 cut(s) 907, 1050
BseBI CCWGG 2 cut(s) 638, 1125
BseDI CCNNGG 3 cut(s) 466, 882, 981
BseGI GGATG 2 cut(s) 835, 894
BseLI CCNNNNNNNGG 4 cut(s) 84, 986, 1045, 1299
BseMII CTCAG 6 cut(s) 386, 654, 810, 858, 1017, 1137
BseNI ACTGG 2 cut(s) 907, 1050
BseRI GAGGAG 1 cut(s) 113
BseSI GKGCMC 1 cut(s) 1290
BseXI GCAGC 2 cut(s) 316, 503
BseYI CCCAGC 2 cut(s) 21, 253
BsgI GTGCAG 1 cut(s) 699
BshFI GGCC 2 cut(s) 471, 636
BshNI GGYRCC 1 cut(s) 1220
BsiHKAI GWGCWC 1 cut(s) 1290
BslFI GGGAC 2 cut(s) 61, 1183
BslI CCNNNNNNNGG 4 cut(s) 84, 986, 1045, 1299
BsmAI GTCTC 4 cut(s) 779, 1029, 1043, 1343
BsmFI GGGAC 2 cut(s) 61, 1183
BsnI GGCC 2 cut(s) 471, 636
Bso31I GGTCTC 2 cut(s) 1029, 1043
Bsp1286I GDGCHC 1 cut(s) 1290
Bsp143I GATC 4 cut(s) 197, 280, 292, 643
Bsp19I CCATGG 1 cut(s) 466
BspACI CCGC 1 cut(s) 633
BspANI GGCC 2 cut(s) 471, 636
BspCNI CTCAG 6 cut(s) 385, 655, 811, 859, 1016, 1138
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 3 cut(s) 258, 880, 1222
BspMAI CTGCAG 2 cut(s) 334, 493
BspPI GGATC 2 cut(s) 205, 288
BspT107I GGYRCC 1 cut(s) 1220
BspTI CTTAAG 1 cut(s) 1109
BspTNI GGTCTC 2 cut(s) 1029, 1043
BsrI ACTGG 2 cut(s) 907, 1050
BssECI CCNNGG 3 cut(s) 466, 882, 981
BssMI GATC 4 cut(s) 197, 280, 292, 643
BssNI GRCGYC 1 cut(s) 1422
BssT1I CCWWGG 3 cut(s) 466, 882, 981
Bst2UI CCWGG 2 cut(s) 638, 1125
Bst4CI ACNGT 5 cut(s) 211, 403, 541, 931, 1214
BstACI GRCGYC 1 cut(s) 1422
BstAFI CTTAAG 1 cut(s) 1109
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 9 cut(s) 264, 372, 663, 819, 867, 1003, 1021, 1146, 1182
BstDSI CCRYGG 1 cut(s) 466
BstF5I GGATG 2 cut(s) 835, 894
BstHHI GCGC 1 cut(s) 1298
BstKTI GATC 4 cut(s) 200, 283, 295, 646
BstMAI GTCTC 4 cut(s) 779, 1029, 1043, 1343
BstMBI GATC 4 cut(s) 197, 280, 292, 643
BstNI CCWGG 2 cut(s) 638, 1125
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 636, 1123
BstSFI CTRYAG 3 cut(s) 330, 489, 579
BstSLI GKGCMC 1 cut(s) 1290
BstV1I GCAGC 2 cut(s) 316, 503
BstV2I GAAGAC 1 cut(s) 657
BstX2I RGATCY 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 889
BstYI RGATCY 1 cut(s) 197
BsuI GTATCC 1 cut(s) 919
BsuRI GGCC 2 cut(s) 471, 636
BtgI CCRYGG 1 cut(s) 466
BtgZI GCGATG 1 cut(s) 1248
BtsCI GGATG 2 cut(s) 835, 894
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 5 cut(s) 207, 486, 552, 900, 1043
Cac8I GCNNGC 1 cut(s) 6
CciI TCATGA 1 cut(s) 607
CfoI GCGC 1 cut(s) 1298
Cfr13I GGNCC 1 cut(s) 257
CseI GACGC 1 cut(s) 1430
Csp6I GTAC 5 cut(s) 212, 399, 915, 1221, 1414
CspCI CAANNNNNGTGG 2 cut(s) 889, 924
CviAII CATG 5 cut(s) 5, 467, 608, 940, 1088
CviQI GTAC 5 cut(s) 212, 399, 915, 1221, 1414
DdeI CTNAG 9 cut(s) 264, 372, 663, 819, 867, 1003, 1021, 1146, 1182
DpnI GATC 4 cut(s) 199, 282, 294, 645
DpnII GATC 4 cut(s) 197, 280, 292, 643
DraI TTTAAA 1 cut(s) 1408
EaeI YGGCCR 2 cut(s) 469, 634
Eco130I CCWWGG 3 cut(s) 466, 882, 981
Eco31I GGTCTC 2 cut(s) 1029, 1043
Eco47I GGWCC 1 cut(s) 257
Eco57I CTGAAG 2 cut(s) 84, 591
EcoO109I RGGNCCY 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 636, 1123
EcoT14I CCWWGG 3 cut(s) 466, 882, 981
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 466, 882, 981
FaeI CATG 5 cut(s) 8, 470, 611, 943, 1091
FalI AAGNNNNNCTT 6 cut(s) 685, 717, 1245, 1277, 1306, 1338
FaqI GGGAC 2 cut(s) 61, 1183
FatI CATG 5 cut(s) 4, 466, 607, 939, 1087
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 3 cut(s) 330, 492, 634
FokI GGATG 2 cut(s) 842, 901
Fsp4HI GCNGC 3 cut(s) 330, 492, 634
FspBI CTAG 5 cut(s) 44, 48, 744, 810, 1362
GlaI GCGC 1 cut(s) 1297
GluI GCNGC 3 cut(s) 330, 492, 634
GsaI CCCAGC 2 cut(s) 25, 257
GsuI CTGGAG 4 cut(s) 1067, 1182, 1197, 1276
HaeIII GGCC 2 cut(s) 471, 636
HgaI GACGC 1 cut(s) 1430
HhaI GCGC 1 cut(s) 1298
Hin1I GRCGYC 1 cut(s) 1422
Hin1II CATG 5 cut(s) 8, 470, 611, 943, 1091
Hin6I GCGC 1 cut(s) 1296
HinP1I GCGC 1 cut(s) 1296
HinfI GANTC 6 cut(s) 59, 142, 368, 457, 575, 1343
HphI GGTGA 1 cut(s) 982
Hpy166II GTNNAC 2 cut(s) 441, 1288
Hpy8I GTNNAC 2 cut(s) 441, 1288
HpyAV CCTTC 7 cut(s) 59, 63, 100, 556, 1067, 1160, 1376
HpyCH4III ACNGT 5 cut(s) 211, 403, 541, 931, 1214
HpyCH4IV ACGT 1 cut(s) 307
HpyCH4V TGCA 8 cut(s) 4, 275, 332, 491, 532, 680, 1288, 1306
HpyF3I CTNAG 9 cut(s) 264, 372, 663, 819, 867, 1003, 1021, 1146, 1182
HpySE526I ACGT 1 cut(s) 307
Hsp92I GRCGYC 1 cut(s) 1422
Hsp92II CATG 5 cut(s) 8, 470, 611, 943, 1091
HspAI GCGC 1 cut(s) 1296
KpnI GGTACC 1 cut(s) 1224
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 4 cut(s) 197, 280, 292, 643
LmnI GCTCC 2 cut(s) 1178, 1201
Lsp1109I GCAGC 2 cut(s) 316, 503
LweI GCATC 1 cut(s) 368
MaeI CTAG 5 cut(s) 44, 48, 744, 810, 1362
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 4 cut(s) 535, 754, 805, 1242
MalI GATC 4 cut(s) 199, 282, 294, 645
MboI GATC 4 cut(s) 197, 280, 292, 643
MboII GAAGA 4 cut(s) 571, 584, 662, 1283
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 1 cut(s) 1290
MluCI AATT 8 cut(s) 81, 219, 595, 842, 850, 1026, 1161, 1366
MmeI TCCRAC 2 cut(s) 903, 967
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 4 cut(s) 171, 797, 1110, 1407
MspCI CTTAAG 1 cut(s) 1109
MspR9I CCNGG 2 cut(s) 638, 1125
MvaI CCWGG 2 cut(s) 638, 1125
NcoI CCATGG 1 cut(s) 466
NdeII GATC 4 cut(s) 197, 280, 292, 643
NlaIII CATG 5 cut(s) 8, 470, 611, 943, 1091
NlaIV GGNNCC 3 cut(s) 258, 880, 1222
NmeAIII GCCGAG 1 cut(s) 497
NmuCI GTSAC 2 cut(s) 754, 1242
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeI GCATGC 1 cut(s) 8
PagI TCATGA 1 cut(s) 607
PfeI GAWTC 6 cut(s) 59, 142, 368, 457, 575, 1343
PkrI GCNGC 3 cut(s) 331, 493, 635
PpuMI RGGWCCY 1 cut(s) 257
PsiI TTATAA 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 2 cut(s) 636, 1123
PspFI CCCAGC 2 cut(s) 21, 253
PspGI CCWGG 2 cut(s) 636, 1123
PspN4I GGNNCC 3 cut(s) 258, 880, 1222
PspPI GGNCC 1 cut(s) 257
PspPPI RGGWCCY 1 cut(s) 257
PstI CTGCAG 2 cut(s) 334, 493
PsuI RGATCY 1 cut(s) 197
RsaI GTAC 5 cut(s) 213, 400, 916, 1222, 1415
RsaNI GTAC 5 cut(s) 212, 399, 915, 1221, 1414
SaqAI TTAA 4 cut(s) 171, 797, 1110, 1407
SatI GCNGC 3 cut(s) 330, 492, 634
Sau3AI GATC 4 cut(s) 197, 280, 292, 643
Sau96I GGNCC 1 cut(s) 257
ScrFI CCNGG 2 cut(s) 638, 1125
SduI GDGCHC 1 cut(s) 1290
SfaNI GCATC 1 cut(s) 368
SfcI CTRYAG 3 cut(s) 330, 489, 579
SinI GGWCC 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 1109
SmoI CTYRAG 1 cut(s) 1109
SphI GCATGC 1 cut(s) 8
Sse9I AATT 8 cut(s) 81, 219, 595, 842, 850, 1026, 1161, 1366
SsiI CCGC 1 cut(s) 633
SspI AATATT 1 cut(s) 1081
SspMI CTAG 5 cut(s) 44, 48, 744, 810, 1362
StyD4I CCNGG 2 cut(s) 636, 1123
StyI CCWWGG 3 cut(s) 466, 882, 981
TaaI ACNGT 5 cut(s) 211, 403, 541, 931, 1214
TaiI ACGT 1 cut(s) 310
TaqI TCGA 5 cut(s) 323, 462, 954, 1341, 1378
TasI AATT 8 cut(s) 81, 219, 595, 842, 850, 1026, 1161, 1366
TatI WGTACW 1 cut(s) 1413
TauI GCSGC 1 cut(s) 636
TfiI GAWTC 6 cut(s) 59, 142, 368, 457, 575, 1343
Tru1I TTAA 4 cut(s) 171, 797, 1110, 1407
Tru9I TTAA 4 cut(s) 171, 797, 1110, 1407
TscAI CASTG 5 cut(s) 214, 493, 552, 907, 1050
TseFI GTSAC 2 cut(s) 754, 1242
TseI GCWGC 2 cut(s) 329, 491
Tsp45I GTSAC 2 cut(s) 754, 1242
TspDTI ATGAA 5 cut(s) 72, 624, 956, 1104, 1290
TspRI CASTG 5 cut(s) 214, 493, 552, 907, 1050
Vha464I CTTAAG 1 cut(s) 1109
VneI GTGCAC 1 cut(s) 1286
VpaK11BI GGWCC 1 cut(s) 257
XapI RAATTY 3 cut(s) 81, 850, 1366
XbaI TCTAGA 1 cut(s) 1361
XceI RCATGY 1 cut(s) 8
XcmI CCANNNNNNNNNTGG 2 cut(s) 188, 946
XspI CTAG 5 cut(s) 44, 48, 744, 810, 1362
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.