MD07G1075000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006827
Physical Location & Seq
Reverse (-)
7187795 .. 7192304
4510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1075000.v1.1.491

Sequence Viewer

Length: 1362 bp
ATGGTTGAATCCTTTATAAAGAAGTACCAGAAACTAAACAATGGGAGTTTCCCATCACTTAATCTGACCCACAAAGAAGTTGGTGGATCTTTCTACACAGTACGAGAAATTGTCCGAGATATAATCCAGGAGAATAGAGTGCTTGGTCCTGCTAAGTTTACTGTAGAGGAGCAGACTGTTGATCAGTTCTGGGAACTCAATCCATTGGGAACCATTGCTGCAGAACCACAAAATTCTTTGTCAATGTCATCAAATGAATCACAGTTCATCACCAATCAAAATCAGGGTGCAATTGAAGAACTGGTTTTCGCTTCTGATGGGCATTTAGTTAGACCTGAACGTCAGATGTTTGAGAATGGGCGAATCATCAACGGTACTCAAGTAGAAGTGAATGACAGAGAATTTAATGAACTGAAAAGTACAGATTTAGAAGTAAATGGACCAGTGGAAATAGAGAAGCATGTTGCTGTTGAATCTATAGTTTCTAATGAGCATTGTACTGAGCCTGAATATCAGAGTTTTGGAAGTGGACAAATCAATGGCAGCGAGGTAGATATGAAAGTGAAAGAAAGGCAGGTACAAACATGTACAGAGCCGCAACCAGTTGAACCTATGGAAGCAAAGAAGAATGGTGAAGAAGTTCTGGCAACATCTAGGCCTAAGGTGGCAGCAGATGTAATAGTGGAGACATTCCCATTAAAGCCAGTAACTAGGAAACCTGAGAGCTTGGATAGAAGTTTACAAGTAGTAACAGATTTGACTAAGTCTACTGAGGACAAAGGAACTAGAGCGATGGAGTCAGCTTGTGTTGATAGTTCGCCAGTGGATAGATTAAGTTCCATGAAGAACATGGATGATAATGTAGCAATAAATCTTTCAAGTCCATTGGTTGAAAGCAAATCCAACCCGGTGGATGAAGCACTAGGACATGCCATAGATCCATCACTTGAAAGCTCAAATTCCTCAACCTTTAATGAAGGTATTGTGCATGAAAAAGGAAGCACAGACCTTAGTGTCAAATCACCTCATAAAGATGTTCCAACCTCTGAGATCTTTGAACAAAGCCAGCTGACTGAGGGATCTAAGGCTGATAAAGCTCCAGATAGTTTACATGCAAAGAATATCAACGGTACCAGTGGTAGCAGCGAGCTGTTGAAAACCAAAGAGGTGCTTGTGGTTGAAGATGAAGTTGATGTCCAGACCAGCAGCAGTTTGACGAAGGGAAGCAAACCAACTTTAGACAGAATTAATCTTGAATCATGGGAAGGTGCATCTAAAAAGTCCAAAAGACCAGAAGGTAACCCACTTTGGGATGTATTTAAAGCTTTTTTAGACGCCTTTGTGAAATTCTGGTCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

454

Amino Acids

50.02

Weight (kDa)

4.81

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_AT3G52170 PF25896 1 - 42 1.4e-19 Helix-turn-helix domain in AT3G52170-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
AasI GACNNNNNNGTC 2 cut(s) 339, 1013
Acc36I ACCTGC 1 cut(s) 565
Acc65I GGTACC 1 cut(s) 1130
AccB1I GGYRCC 1 cut(s) 1130
AccI GTMKAC 1 cut(s) 767
AciI CCGC 1 cut(s) 596
AclWI GGATC 3 cut(s) 94, 932, 1087
AcsI RAATTY 4 cut(s) 232, 401, 958, 1346
AcyI GRCGYC 1 cut(s) 1335
AfaI GTAC 8 cut(s) 26, 102, 376, 421, 499, 579, 589, 1132
AfiI CCNNNNNNNGG 1 cut(s) 1309
AflIII ACRYGT 1 cut(s) 584
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 7 cut(s) 726, 803, 954, 1069, 1097, 1150, 1325
AluI AGCT 7 cut(s) 726, 803, 954, 1069, 1097, 1150, 1325
Alw26I GTCTC 1 cut(s) 680
AlwI GGATC 3 cut(s) 94, 932, 1087
AoxI GGCC 1 cut(s) 656
ApeKI GCWGC 5 cut(s) 218, 543, 668, 1143, 1206
ApoI RAATTY 4 cut(s) 232, 401, 958, 1346
AseI ATTAAT 1 cut(s) 1248
Asp700I GAANNNNTTC 1 cut(s) 639
Asp718I GGTACC 1 cut(s) 1130
AspS9I GGNCC 2 cut(s) 146, 440
AsuC2I CCSGG 1 cut(s) 908
AsuHPI GGTGA 3 cut(s) 262, 644, 1014
AvaII GGWCC 2 cut(s) 146, 440
AxyI CCTNAGG 1 cut(s) 660
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BanI GGYRCC 1 cut(s) 1130
BbvI GCAGC 5 cut(s) 205, 555, 680, 1155, 1218
BccI CCATC 4 cut(s) 61, 311, 787, 949
BciT130I CCWGG 1 cut(s) 128
BclI TGATCA 1 cut(s) 181
BcnI CCSGG 1 cut(s) 908
BcoDI GTCTC 1 cut(s) 680
BfaI CTAG 4 cut(s) 654, 711, 786, 923
BfmI CTRYAG 3 cut(s) 162, 219, 477
BfuAI ACCTGC 1 cut(s) 565
BglII AGATCT 1 cut(s) 1050
BisI GCNGC 6 cut(s) 219, 544, 596, 669, 1144, 1207
BlsI GCNGC 6 cut(s) 220, 545, 597, 670, 1145, 1208
Bme1390I CCNGG 2 cut(s) 128, 908
Bme18I GGWCC 2 cut(s) 146, 440
BmgT120I GGNCC 2 cut(s) 146, 440
BmiI GGNNCC 2 cut(s) 211, 1132
BmrFI CCNGG 2 cut(s) 128, 908
BmsI GCATC 1 cut(s) 1280
BpmI CTGGAG 1 cut(s) 1083
BpuEI CTTGAG 1 cut(s) 363
BpuMI CCSGG 1 cut(s) 908
BsaHI GRCGYC 1 cut(s) 1335
Bsc4I CCNNNNNNNGG 1 cut(s) 1309
Bse1I ACTGG 6 cut(s) 306, 443, 602, 704, 821, 1134
Bse21I CCTNAGG 1 cut(s) 660
Bse3DI GCAATG 1 cut(s) 213
BseBI CCWGG 1 cut(s) 128
BseGI GGATG 3 cut(s) 859, 919, 1318
BseLI CCNNNNNNNGG 1 cut(s) 1309
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 5 cut(s) 492, 711, 762, 1038, 1065
BseNI ACTGG 6 cut(s) 306, 443, 602, 704, 821, 1134
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 5 cut(s) 205, 555, 680, 1155, 1218
BshFI GGCC 1 cut(s) 658
BshNI GGYRCC 1 cut(s) 1130
BsiSI CCGG 1 cut(s) 908
BslI CCNNNNNNNGG 1 cut(s) 1309
BsmAI GTCTC 1 cut(s) 680
BsnI GGCC 1 cut(s) 658
Bsp1407I TGTACA 1 cut(s) 587
Bsp143I GATC 5 cut(s) 86, 181, 937, 1050, 1079
BspACI CCGC 1 cut(s) 596
BspANI GGCC 1 cut(s) 658
BspCNI CTCAG 5 cut(s) 493, 712, 763, 1039, 1066
BspLI GGNNCC 2 cut(s) 211, 1132
BspMAI CTGCAG 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 3 cut(s) 94, 932, 1087
BspT107I GGYRCC 1 cut(s) 1130
BsrDI GCAATG 1 cut(s) 213
BsrGI TGTACA 1 cut(s) 587
BsrI ACTGG 6 cut(s) 306, 443, 602, 704, 821, 1134
BssMI GATC 5 cut(s) 86, 181, 937, 1050, 1079
BssNI GRCGYC 1 cut(s) 1335
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 6 cut(s) 100, 163, 178, 264, 374, 1130
BstACI GRCGYC 1 cut(s) 1335
BstAUI TGTACA 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 1067, 1148
BstEII GGTNACC 1 cut(s) 1298
BstF5I GGATG 3 cut(s) 859, 919, 1318
BstKTI GATC 5 cut(s) 89, 184, 940, 1053, 1082
BstMAI GTCTC 1 cut(s) 680
BstMBI GATC 5 cut(s) 86, 181, 937, 1050, 1079
BstMWI GCNNNNNNNGC 1 cut(s) 1094
BstNI CCWGG 1 cut(s) 128
BstNSI RCATGY 4 cut(s) 464, 588, 932, 1115
BstPI GGTNACC 1 cut(s) 1298
BstSCI CCNGG 2 cut(s) 126, 906
BstSFI CTRYAG 3 cut(s) 162, 219, 477
BstV1I GCAGC 5 cut(s) 205, 555, 680, 1155, 1218
BstX2I RGATCY 4 cut(s) 86, 937, 1050, 1079
BstXI CCANNNNNNTGG 1 cut(s) 910
BstYI RGATCY 4 cut(s) 86, 937, 1050, 1079
Bsu36I CCTNAGG 1 cut(s) 660
BsuRI GGCC 1 cut(s) 658
BtgZI GCGATG 1 cut(s) 806
BtsCI GGATG 3 cut(s) 859, 919, 1318
BtsIMutI CAGTG 3 cut(s) 450, 828, 1141
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 2 cut(s) 1067, 1148
Cfr13I GGNCC 2 cut(s) 146, 440
CseI GACGC 1 cut(s) 1343
Csp6I GTAC 8 cut(s) 25, 101, 375, 420, 498, 578, 588, 1131
CviAII CATG 8 cut(s) 461, 585, 841, 850, 929, 989, 1112, 1260
CviQI GTAC 8 cut(s) 25, 101, 375, 420, 498, 578, 588, 1131
DpnI GATC 5 cut(s) 88, 183, 939, 1052, 1081
DpnII GATC 5 cut(s) 86, 181, 937, 1050, 1079
DraI TTTAAA 1 cut(s) 1321
DrdI GACNNNNNNGTC 2 cut(s) 339, 1013
DseDI GACNNNNNNGTC 2 cut(s) 339, 1013
Eco147I AGGCCT 1 cut(s) 658
Eco47I GGWCC 2 cut(s) 146, 440
Eco81I CCTNAGG 1 cut(s) 660
Eco91I GGTNACC 1 cut(s) 1298
EcoO65I GGTNACC 1 cut(s) 1298
EcoRII CCWGG 1 cut(s) 126
FaeI CATG 8 cut(s) 464, 588, 844, 853, 932, 992, 1115, 1263
FalI AAGNNNNNCTT 2 cut(s) 1155, 1187
FatI CATG 8 cut(s) 460, 584, 840, 849, 928, 988, 1111, 1259
FbaI TGATCA 1 cut(s) 181
FblI GTMKAC 1 cut(s) 767
Fnu4HI GCNGC 6 cut(s) 219, 544, 596, 669, 1144, 1207
FokI GGATG 3 cut(s) 866, 926, 1325
Fsp4HI GCNGC 6 cut(s) 219, 544, 596, 669, 1144, 1207
FspBI CTAG 4 cut(s) 654, 711, 786, 923
GluI GCNGC 6 cut(s) 219, 544, 596, 669, 1144, 1207
GsuI CTGGAG 1 cut(s) 1083
HaeIII GGCC 1 cut(s) 658
HapII CCGG 1 cut(s) 908
HgaI GACGC 1 cut(s) 1343
Hin1I GRCGYC 1 cut(s) 1335
Hin1II CATG 8 cut(s) 464, 588, 844, 853, 932, 992, 1115, 1263
HindIII AAGCTT 1 cut(s) 1323
HinfI GANTC 6 cut(s) 8, 257, 363, 473, 797, 1256
HpaII CCGG 1 cut(s) 908
HphI GGTGA 3 cut(s) 262, 644, 1014
Hpy166II GTNNAC 5 cut(s) 159, 530, 740, 768, 1109
Hpy188I TCNGA 7 cut(s) 66, 116, 316, 345, 516, 1048, 1357
Hpy188III TCNNGA 3 cut(s) 1100, 1198, 1253
Hpy8I GTNNAC 5 cut(s) 159, 530, 740, 768, 1109
HpyAV CCTTC 4 cut(s) 971, 1213, 1259, 1289
HpyCH4III ACNGT 6 cut(s) 100, 163, 178, 264, 374, 1130
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 5 cut(s) 221, 290, 988, 1115, 1271
HpyF10VI GCNNNNNNNGC 1 cut(s) 1094
HpySE526I ACGT 1 cut(s) 340
Hsp92I GRCGYC 1 cut(s) 1335
Hsp92II CATG 8 cut(s) 464, 588, 844, 853, 932, 992, 1115, 1263
KpnI GGTACC 1 cut(s) 1134
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 5 cut(s) 86, 181, 937, 1050, 1079
LmnI GCTCC 2 cut(s) 169, 1102
Lsp1109I GCAGC 5 cut(s) 205, 555, 680, 1155, 1218
LweI GCATC 1 cut(s) 1280
MaeI CTAG 4 cut(s) 654, 711, 786, 923
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 3 cut(s) 706, 748, 1298
MalI GATC 5 cut(s) 88, 183, 939, 1052, 1081
MboI GATC 5 cut(s) 86, 181, 937, 1050, 1079
MboII GAAGA 5 cut(s) 308, 637, 647, 856, 1193
MfeI CAATTG 1 cut(s) 291
MflI RGATCY 4 cut(s) 86, 937, 1050, 1079
MluCI AATT 7 cut(s) 108, 232, 291, 401, 958, 1245, 1346
MlyI GAGTC 1 cut(s) 806
MmeI TCCRAC 2 cut(s) 927, 1064
MnlI CCTC 8 cut(s) 160, 541, 766, 973, 1035, 1054, 1069, 1159
MroXI GAANNNNTTC 1 cut(s) 639
MseI TTAA 7 cut(s) 60, 405, 698, 833, 972, 1248, 1320
MslI CAYNNNNRTG 1 cut(s) 1032
MspA1I CMGCKG 1 cut(s) 1069
MspI CCGG 1 cut(s) 908
MspR9I CCNGG 2 cut(s) 128, 908
MunI CAATTG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 128
MwoI GCNNNNNNNGC 1 cut(s) 1094
NciI CCSGG 1 cut(s) 908
NdeII GATC 5 cut(s) 86, 181, 937, 1050, 1079
NlaIII CATG 8 cut(s) 464, 588, 844, 853, 932, 992, 1115, 1263
NlaIV GGNNCC 2 cut(s) 211, 1132
NspI RCATGY 4 cut(s) 464, 588, 932, 1115
PceI AGGCCT 1 cut(s) 658
PciI ACATGT 1 cut(s) 584
PdmI GAANNNNTTC 1 cut(s) 639
PfeI GAWTC 5 cut(s) 8, 257, 363, 473, 1256
PflFI GACNNNGTC 1 cut(s) 763
PfoI TCCNGGA 1 cut(s) 126
PkrI GCNGC 6 cut(s) 220, 545, 597, 670, 1145, 1208
PleI GAGTC 1 cut(s) 805
PpsI GAGTC 1 cut(s) 805
PscI ACATGT 1 cut(s) 584
PshBI ATTAAT 1 cut(s) 1248
PsiI TTATAA 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 126
PspEI GGTNACC 1 cut(s) 1298
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 2 cut(s) 211, 1132
PspPI GGNCC 2 cut(s) 146, 440
PstI CTGCAG 1 cut(s) 223
PsuI RGATCY 4 cut(s) 86, 937, 1050, 1079
PsyI GACNNNGTC 1 cut(s) 763
PvuII CAGCTG 1 cut(s) 1069
RsaI GTAC 8 cut(s) 26, 102, 376, 421, 499, 579, 589, 1132
RsaNI GTAC 8 cut(s) 25, 101, 375, 420, 498, 578, 588, 1131
RseI CAYNNNNRTG 1 cut(s) 1032
SaqAI TTAA 7 cut(s) 60, 405, 698, 833, 972, 1248, 1320
SatI GCNGC 6 cut(s) 219, 544, 596, 669, 1144, 1207
Sau3AI GATC 5 cut(s) 86, 181, 937, 1050, 1079
Sau96I GGNCC 2 cut(s) 146, 440
SchI GAGTC 1 cut(s) 806
ScrFI CCNGG 2 cut(s) 128, 908
SfaNI GCATC 1 cut(s) 1280
SfcI CTRYAG 3 cut(s) 162, 219, 477
SinI GGWCC 2 cut(s) 146, 440
SmiMI CAYNNNNRTG 1 cut(s) 1032
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
Sse9I AATT 7 cut(s) 108, 232, 291, 401, 958, 1245, 1346
SseBI AGGCCT 1 cut(s) 658
SsiI CCGC 1 cut(s) 596
SspMI CTAG 4 cut(s) 654, 711, 786, 923
StuI AGGCCT 1 cut(s) 658
StyD4I CCNGG 2 cut(s) 126, 906
TaaI ACNGT 6 cut(s) 100, 163, 178, 264, 374, 1130
TaiI ACGT 1 cut(s) 343
TasI AATT 7 cut(s) 108, 232, 291, 401, 958, 1245, 1346
TatI WGTACW 3 cut(s) 419, 497, 587
TauI GCSGC 1 cut(s) 598
TfiI GAWTC 5 cut(s) 8, 257, 363, 473, 1256
Tru1I TTAA 7 cut(s) 60, 405, 698, 833, 972, 1248, 1320
Tru9I TTAA 7 cut(s) 60, 405, 698, 833, 972, 1248, 1320
TscAI CASTG 3 cut(s) 450, 828, 1141
TseI GCWGC 5 cut(s) 218, 543, 668, 1143, 1206
TspDTI ATGAA 9 cut(s) 256, 270, 423, 572, 857, 930, 990, 1005, 1200
TspRI CASTG 3 cut(s) 450, 828, 1141
Tth111I GACNNNGTC 1 cut(s) 763
VpaK11BI GGWCC 2 cut(s) 146, 440
VspI ATTAAT 1 cut(s) 1248
XapI RAATTY 4 cut(s) 232, 401, 958, 1346
XceI RCATGY 4 cut(s) 464, 588, 932, 1115
XcmI CCANNNNNNNNNTGG 2 cut(s) 77, 847
XmiI GTMKAC 1 cut(s) 767
XmnI GAANNNNTTC 1 cut(s) 639
XspI CTAG 4 cut(s) 654, 711, 786, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.