pycom07g05890

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006827
Physical Location & Seq
Reverse (-)
5209202 .. 5212011
2810 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g05890.1

Sequence Viewer

Length: 1482 bp
ATGCATTCTGTAAAGGGTAGTTGGGTTGGGCAGACATTTGCCCTTGCTAAAAACAATGAATCTGAAGGAAGGAGATCCAGAATTCGGCGTTCAAAGGAAGAGAGGAAGGCAATGGTTGAATCCTTTATAAAGAAGTACCAGAAACTAAACAATGGGAGTTTCCCGTCACTTAATCTTACCCACAAAGAAGTTGGTGGATCTTTCTACACAGTACGAGAAATTGTCCGAGATATAATCCAGGAGAATAGAGTGCTTGGTCCTGCTAAGTTCACTGTAGAGGAGCAGACCGTTGACCAGTTCTGGGAACACAATCCATTGGGAACCATTGCTGCAGAACCACAAAATTCTTTGTCAGTGTCATCAAATGAATCACAGTTTGTCACCAATAAAAATCAGGGTGCAATTGAAGAACTGGTTTTCGCATCTGATGGGCATTTAGTTAGACCTGAAAGTCAGATGTTTGAGAATGGGCGAATGATCAACGCTACTCAAGTAGAAGTGAATGACAGAGAATTTAATGAATTGAAAAGTACAGAGTTAGAAGTAAATGGACCAGTGGAAATAGAGAAGCATGTTGCTGTTGAATCTATAGTTTCTAATGGGCATTGTACTGAGCCTGAATATCTGAGTTTTGGAAGTGGACAAATCAATGGCAGCGAGGTAGATATGAAAGTGAAAGAAAGGGAGGAACAAACATATACAGAGCTGCAAACAGTTGAACCTTTGGAAGCAAAGAAGAATGGTGAAGAAGTTCTGGCAACATCTAGGCCTAAGGTGACTCCTATAGCAGCAGATGTAATGGTGGAGACATTCCCATTAAAGCCGGTAACTAGGAAACCTGAGAGCTTGGATGGAAGTTTACAAGTAGTAGCAGATTTGACTAAGTCTACTGAGGACAAAGGAACTAGAGCGATGGAGTCAGCTTGTGTTGATAGTTCGCCGGTGGATACATTGAGTTCCATGAAGGGCATGGATGATAATGTAGAGATAAATCTTTCAAGTCCATTGGTTGAAAACAAATCCAGCCCAGTGGATGAAGCACTAGGACATGCCATAGATCGATCACTTGAAAGCTCAAATTCCTCAACCTTTAATGAAGGTATTGTGCATGAAACAGGAAGCACAGACCTTAGTGTCAAATCACCTCATAAAGATGTTCCCACCTCAGAGATCTTTGAACAAAGCCAGCTGACTGAGGGATCTAAGGCTGATAAAGCTCCAGATAGTTTACATGCAAAGAATATCAACGGTACCAGTGGTAGCAGCGAGCTGTTGAAAACCAAAGAGGTGCTTGTGGTTGAAGATGAAGTTGATGTCCAGACCAGCGGCAGTTTGATGAAAGGAAGCAAACCAACTTTAGACAGAATTAATCTTGAATCATGGGAAGGTGCATCTAAAAAGTCCAAAAGACCAGAAGGTAACCCACTTTGGGATGTATTTAAAGCGTTTTTAGACGCCTTTGTGAAATTCTGGTCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

494

Amino Acids

54.42

Weight (kDa)

4.98

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_AT3G52170 PF25896 31 - 79 4.2e-24 Helix-turn-helix domain in AT3G52170-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
AasI GACNNNNNNGTC 2 cut(s) 450, 1133
Acc65I GGTACC 1 cut(s) 1250
AccB1I GGYRCC 1 cut(s) 1250
AccI GTMKAC 1 cut(s) 887
AciI CCGC 1 cut(s) 1326
AclWI GGATC 3 cut(s) 69, 205, 1207
AcsI RAATTY 5 cut(s) 81, 343, 512, 1078, 1466
AcuI CTGAAG 1 cut(s) 84
AcyI GRCGYC 1 cut(s) 1455
AfaI GTAC 5 cut(s) 137, 213, 532, 610, 1252
AfiI CCNNNNNNNGG 3 cut(s) 84, 301, 1429
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 7 cut(s) 706, 846, 923, 1074, 1189, 1217, 1270
AluI AGCT 7 cut(s) 706, 846, 923, 1074, 1189, 1217, 1270
Alw26I GTCTC 1 cut(s) 800
AlwI GGATC 3 cut(s) 69, 205, 1207
AoxI GGCC 1 cut(s) 767
ApeKI GCWGC 5 cut(s) 329, 654, 706, 788, 1263
ApoI RAATTY 5 cut(s) 81, 343, 512, 1078, 1466
AseI ATTAAT 1 cut(s) 1368
Asp700I GAANNNNTTC 1 cut(s) 750
Asp718I GGTACC 1 cut(s) 1250
AspS9I GGNCC 2 cut(s) 257, 551
AsuHPI GGTGA 4 cut(s) 373, 755, 787, 1134
AvaII GGWCC 2 cut(s) 257, 551
AxyI CCTNAGG 1 cut(s) 771
BanI GGYRCC 1 cut(s) 1250
BbvI GCAGC 5 cut(s) 316, 666, 693, 800, 1275
BccI CCATC 3 cut(s) 422, 845, 907
BciT130I CCWGG 1 cut(s) 239
BciVI GTATCC 1 cut(s) 940
BclI TGATCA 1 cut(s) 477
BcoDI GTCTC 1 cut(s) 800
BfaI CTAG 4 cut(s) 765, 831, 906, 1043
BfmI CTRYAG 4 cut(s) 273, 330, 588, 783
BfuI GTATCC 1 cut(s) 940
BglII AGATCT 1 cut(s) 1170
BisI GCNGC 6 cut(s) 330, 655, 707, 789, 1264, 1327
BlsI GCNGC 6 cut(s) 331, 656, 708, 790, 1265, 1328
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 2 cut(s) 257, 551
BmgT120I GGNCC 2 cut(s) 257, 551
BmiI GGNNCC 2 cut(s) 322, 1252
BmrFI CCNGG 1 cut(s) 239
BmrI ACTGGG 1 cut(s) 1022
BmsI GCATC 2 cut(s) 431, 1400
BmuI ACTGGG 1 cut(s) 1022
BpmI CTGGAG 1 cut(s) 1203
BpuEI CTTGAG 1 cut(s) 474
Bsa29I ATCGAT 1 cut(s) 1060
BsaHI GRCGYC 1 cut(s) 1455
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 301, 1429
Bse118I RCCGGY 2 cut(s) 823, 940
Bse1I ACTGG 5 cut(s) 295, 417, 554, 1028, 1254
Bse21I CCTNAGG 1 cut(s) 771
Bse3DI GCAATG 2 cut(s) 117, 324
BseBI CCWGG 1 cut(s) 239
BseCI ATCGAT 1 cut(s) 1060
BseGI GGATG 4 cut(s) 856, 979, 1039, 1438
BseLI CCNNNNNNNGG 3 cut(s) 84, 301, 1429
BseMI GCAATG 2 cut(s) 117, 324
BseMII CTCAG 6 cut(s) 603, 617, 831, 882, 1179, 1185
BseNI ACTGG 5 cut(s) 295, 417, 554, 1028, 1254
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 5 cut(s) 316, 666, 693, 800, 1275
BshFI GGCC 1 cut(s) 769
BshNI GGYRCC 1 cut(s) 1250
BshVI ATCGAT 1 cut(s) 1060
BsiSI CCGG 2 cut(s) 824, 941
BslI CCNNNNNNNGG 3 cut(s) 84, 301, 1429
BsmAI GTCTC 1 cut(s) 800
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 1 cut(s) 769
Bsp143I GATC 7 cut(s) 74, 197, 477, 1057, 1061, 1170, 1199
BspACI CCGC 1 cut(s) 1326
BspANI GGCC 1 cut(s) 769
BspCNI CTCAG 6 cut(s) 604, 618, 832, 883, 1178, 1186
BspDI ATCGAT 1 cut(s) 1060
BspLI GGNNCC 2 cut(s) 322, 1252
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 3 cut(s) 69, 205, 1207
BspT107I GGYRCC 1 cut(s) 1250
BsrDI GCAATG 2 cut(s) 117, 324
BsrFI RCCGGY 2 cut(s) 823, 940
BsrI ACTGG 5 cut(s) 295, 417, 554, 1028, 1254
BssAI RCCGGY 2 cut(s) 823, 940
BssMI GATC 7 cut(s) 74, 197, 477, 1057, 1061, 1170, 1199
BssNI GRCGYC 1 cut(s) 1455
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 6 cut(s) 211, 274, 289, 375, 715, 1250
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 1455
BstC8I GCNNGC 2 cut(s) 1187, 1268
BstEII GGTNACC 1 cut(s) 1418
BstF5I GGATG 4 cut(s) 856, 979, 1039, 1438
BstKTI GATC 7 cut(s) 77, 200, 480, 1060, 1064, 1173, 1202
BstMAI GTCTC 1 cut(s) 800
BstMBI GATC 7 cut(s) 74, 197, 477, 1057, 1061, 1170, 1199
BstMWI GCNNNNNNNGC 1 cut(s) 1214
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 3 cut(s) 575, 1052, 1235
BstPI GGTNACC 1 cut(s) 1418
BstSCI CCNGG 1 cut(s) 237
BstSFI CTRYAG 4 cut(s) 273, 330, 588, 783
BstV1I GCAGC 5 cut(s) 316, 666, 693, 800, 1275
BstX2I RGATCY 4 cut(s) 74, 197, 1170, 1199
BstXI CCANNNNNNTGG 1 cut(s) 1030
BstYI RGATCY 4 cut(s) 74, 197, 1170, 1199
Bsu15I ATCGAT 1 cut(s) 1060
Bsu36I CCTNAGG 1 cut(s) 771
BsuI GTATCC 1 cut(s) 940
BsuRI GGCC 1 cut(s) 769
BsuTUI ATCGAT 1 cut(s) 1060
BtgZI GCGATG 1 cut(s) 926
BtsCI GGATG 4 cut(s) 856, 979, 1039, 1438
BtsIMutI CAGTG 5 cut(s) 270, 360, 561, 1035, 1261
Cac8I GCNNGC 2 cut(s) 1187, 1268
Cfr10I RCCGGY 2 cut(s) 823, 940
Cfr13I GGNCC 2 cut(s) 257, 551
ClaI ATCGAT 1 cut(s) 1060
CseI GACGC 1 cut(s) 1463
Csp6I GTAC 5 cut(s) 136, 212, 531, 609, 1251
CviAII CATG 7 cut(s) 572, 961, 970, 1049, 1109, 1232, 1380
CviQI GTAC 5 cut(s) 136, 212, 531, 609, 1251
DpnI GATC 7 cut(s) 76, 199, 479, 1059, 1063, 1172, 1201
DpnII GATC 7 cut(s) 74, 197, 477, 1057, 1061, 1170, 1199
DraI TTTAAA 1 cut(s) 1441
DrdI GACNNNNNNGTC 2 cut(s) 450, 1133
DseDI GACNNNNNNGTC 2 cut(s) 450, 1133
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Eco147I AGGCCT 1 cut(s) 769
Eco47I GGWCC 2 cut(s) 257, 551
Eco57I CTGAAG 1 cut(s) 84
Eco81I CCTNAGG 1 cut(s) 771
Eco91I GGTNACC 1 cut(s) 1418
EcoO65I GGTNACC 1 cut(s) 1418
EcoRI GAATTC 1 cut(s) 81
EcoRII CCWGG 1 cut(s) 237
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 7 cut(s) 575, 964, 973, 1052, 1112, 1235, 1383
FalI AAGNNNNNCTT 2 cut(s) 1275, 1307
FatI CATG 7 cut(s) 571, 960, 969, 1048, 1108, 1231, 1379
FbaI TGATCA 1 cut(s) 477
FblI GTMKAC 1 cut(s) 887
Fnu4HI GCNGC 6 cut(s) 330, 655, 707, 789, 1264, 1327
FokI GGATG 4 cut(s) 863, 986, 1046, 1445
Fsp4HI GCNGC 6 cut(s) 330, 655, 707, 789, 1264, 1327
FspBI CTAG 4 cut(s) 765, 831, 906, 1043
GluI GCNGC 6 cut(s) 330, 655, 707, 789, 1264, 1327
GsuI CTGGAG 1 cut(s) 1203
HaeIII GGCC 1 cut(s) 769
HapII CCGG 2 cut(s) 824, 941
HgaI GACGC 1 cut(s) 1463
Hin1I GRCGYC 1 cut(s) 1455
Hin1II CATG 7 cut(s) 575, 964, 973, 1052, 1112, 1235, 1383
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 7 cut(s) 59, 119, 368, 584, 778, 917, 1376
HpaII CCGG 2 cut(s) 824, 941
HphI GGTGA 4 cut(s) 373, 755, 787, 1134
Hpy166II GTNNAC 6 cut(s) 270, 292, 641, 860, 888, 1229
Hpy188I TCNGA 7 cut(s) 64, 227, 427, 456, 627, 1168, 1477
Hpy188III TCNNGA 4 cut(s) 78, 1220, 1318, 1373
Hpy8I GTNNAC 6 cut(s) 270, 292, 641, 860, 888, 1229
HpyAV CCTTC 7 cut(s) 59, 63, 100, 958, 1091, 1379, 1409
HpyCH4III ACNGT 6 cut(s) 211, 274, 289, 375, 715, 1250
HpyCH4V TGCA 7 cut(s) 4, 332, 401, 709, 1108, 1235, 1391
HpyF10VI GCNNNNNNNGC 1 cut(s) 1214
Hsp92I GRCGYC 1 cut(s) 1455
Hsp92II CATG 7 cut(s) 575, 964, 973, 1052, 1112, 1235, 1383
KpnI GGTACC 1 cut(s) 1254
Ksp22I TGATCA 1 cut(s) 477
Kzo9I GATC 7 cut(s) 74, 197, 477, 1057, 1061, 1170, 1199
LmnI GCTCC 2 cut(s) 280, 1222
Lsp1109I GCAGC 5 cut(s) 316, 666, 693, 800, 1275
LweI GCATC 2 cut(s) 431, 1400
MaeI CTAG 4 cut(s) 765, 831, 906, 1043
MaeIII GTNAC 5 cut(s) 165, 379, 775, 826, 1418
MalI GATC 7 cut(s) 76, 199, 479, 1059, 1063, 1172, 1201
MboI GATC 7 cut(s) 74, 197, 477, 1057, 1061, 1170, 1199
MboII GAAGA 5 cut(s) 110, 419, 748, 758, 1313
MfeI CAATTG 1 cut(s) 402
MflI RGATCY 4 cut(s) 74, 197, 1170, 1199
MluCI AATT 9 cut(s) 81, 219, 343, 402, 512, 521, 1078, 1365, 1466
MlyI GAGTC 2 cut(s) 772, 926
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 750
MseI TTAA 6 cut(s) 171, 516, 818, 1092, 1368, 1440
MslI CAYNNNNRTG 1 cut(s) 1152
MspA1I CMGCKG 2 cut(s) 1189, 1326
MspI CCGG 2 cut(s) 824, 941
MspR9I CCNGG 1 cut(s) 239
MunI CAATTG 1 cut(s) 402
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 1214
NdeII GATC 7 cut(s) 74, 197, 477, 1057, 1061, 1170, 1199
NlaIII CATG 7 cut(s) 575, 964, 973, 1052, 1112, 1235, 1383
NlaIV GGNNCC 2 cut(s) 322, 1252
NmuCI GTSAC 3 cut(s) 165, 379, 775
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 575, 1052, 1235
PceI AGGCCT 1 cut(s) 769
PctI GAATGC 1 cut(s) 4
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 5 cut(s) 59, 119, 368, 584, 1376
PflFI GACNNNGTC 1 cut(s) 883
PfoI TCCNGGA 1 cut(s) 237
PkrI GCNGC 6 cut(s) 331, 656, 708, 790, 1265, 1328
PleI GAGTC 2 cut(s) 772, 925
PpsI GAGTC 2 cut(s) 772, 925
PshBI ATTAAT 1 cut(s) 1368
PsiI TTATAA 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 237
PspEI GGTNACC 1 cut(s) 1418
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 2 cut(s) 322, 1252
PspPI GGNCC 2 cut(s) 257, 551
PstI CTGCAG 1 cut(s) 334
PsuI RGATCY 4 cut(s) 74, 197, 1170, 1199
PsyI GACNNNGTC 1 cut(s) 883
PvuII CAGCTG 1 cut(s) 1189
RsaI GTAC 5 cut(s) 137, 213, 532, 610, 1252
RsaNI GTAC 5 cut(s) 136, 212, 531, 609, 1251
RseI CAYNNNNRTG 1 cut(s) 1152
SaqAI TTAA 6 cut(s) 171, 516, 818, 1092, 1368, 1440
SatI GCNGC 6 cut(s) 330, 655, 707, 789, 1264, 1327
Sau3AI GATC 7 cut(s) 74, 197, 477, 1057, 1061, 1170, 1199
Sau96I GGNCC 2 cut(s) 257, 551
SchI GAGTC 2 cut(s) 772, 926
ScrFI CCNGG 1 cut(s) 239
SfaNI GCATC 2 cut(s) 431, 1400
SfcI CTRYAG 4 cut(s) 273, 330, 588, 783
SgrAI CRCCGGYG 1 cut(s) 940
SinI GGWCC 2 cut(s) 257, 551
SmiMI CAYNNNNRTG 1 cut(s) 1152
SmlI CTYRAG 1 cut(s) 489
SmoI CTYRAG 1 cut(s) 489
Sse9I AATT 9 cut(s) 81, 219, 343, 402, 512, 521, 1078, 1365, 1466
SseBI AGGCCT 1 cut(s) 769
SsiI CCGC 1 cut(s) 1326
SspMI CTAG 4 cut(s) 765, 831, 906, 1043
StuI AGGCCT 1 cut(s) 769
StyD4I CCNGG 1 cut(s) 237
TaaI ACNGT 6 cut(s) 211, 274, 289, 375, 715, 1250
TaqI TCGA 1 cut(s) 1060
TasI AATT 9 cut(s) 81, 219, 343, 402, 512, 521, 1078, 1365, 1466
TatI WGTACW 2 cut(s) 530, 608
TauI GCSGC 1 cut(s) 1329
TfiI GAWTC 5 cut(s) 59, 119, 368, 584, 1376
Tru1I TTAA 6 cut(s) 171, 516, 818, 1092, 1368, 1440
Tru9I TTAA 6 cut(s) 171, 516, 818, 1092, 1368, 1440
TscAI CASTG 5 cut(s) 277, 360, 561, 1035, 1261
TseFI GTSAC 3 cut(s) 165, 379, 775
TseI GCWGC 5 cut(s) 329, 654, 706, 788, 1263
Tsp45I GTSAC 3 cut(s) 165, 379, 775
TspRI CASTG 5 cut(s) 277, 360, 561, 1035, 1261
Tth111I GACNNNGTC 1 cut(s) 883
VpaK11BI GGWCC 2 cut(s) 257, 551
VspI ATTAAT 1 cut(s) 1368
XapI RAATTY 5 cut(s) 81, 343, 512, 1078, 1466
XceI RCATGY 3 cut(s) 575, 1052, 1235
XcmI CCANNNNNNNNNTGG 2 cut(s) 188, 967
XmiI GTMKAC 1 cut(s) 887
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 4 cut(s) 765, 831, 906, 1043
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.